CN105969717A - Method for preparing efficient ecological restoration floras for Hg contaminated site from biogas residues - Google Patents
Method for preparing efficient ecological restoration floras for Hg contaminated site from biogas residues Download PDFInfo
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- CN105969717A CN105969717A CN201610370460.2A CN201610370460A CN105969717A CN 105969717 A CN105969717 A CN 105969717A CN 201610370460 A CN201610370460 A CN 201610370460A CN 105969717 A CN105969717 A CN 105969717A
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- CN
- China
- Prior art keywords
- sulfate
- flora
- biogas residue
- hydrargyrum
- solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Abstract
The invention provides a method for preparing efficient ecological restoration floras for an Hg contaminated site from biogas residues. A carbon source and sulfate are added into the biogas residues, sulfate reducing floras in the biogas residues are made to grow rapidly, and meanwhile sulfide is generated; next, mercuric salt is added to consume the sulfide inhibiting growth of sulfate reducing bacteria, the toxicity of the mercuric salt on the sulfate reducing bacteria is greatly lowered due to existence of the sulfide, and meanwhile Hg resistance of the cultured sulfate reducing floras is improved.
Description
Technical field
The present invention provides a kind of and utilizes biogas residue to prepare the method that Hg contaminated site efficient ecological repairs flora, by adding carbon source and sulfate in biogas residue, makes sulfate reduction flora in biogas residue mushroom out, produces sulfide simultaneously；It is subsequently added mercury salt, consumes the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the mercury salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Hg performance improve simultaneously, belong to field of environment protection.
Background technology
Owing to mining, Metallurgical Waste Water arbitrarily discharge, cause a large amount of soil and polluted by Hg.The method processing mercurous soil at present is the most defective.High-temperature melting method cost is high, and power consumption is big.Bioanalysis is long for reaction time, and easily causes the secondary pollution of adventive microbial inoculum.Solidification method effect is unstable, and soil is easily caused secondary pollution by firming agent.The patent No. 201510056406.6 describes a kind of curing containing heavy metal soil, but the method using substantial amounts of material, relatively costly, on the other hand solidification effect is unstable, the most easily there is carbonation in the calcium oxide as added so that alkaline-curing effect reduces.
Summary of the invention
For the deficiencies in the prior art, the present invention provides a kind of and utilizes biogas residue to prepare the method that Hg contaminated site efficient ecological repairs flora, by adding carbon source and sulfate in biogas residue, makes sulfate reduction flora in biogas residue mushroom out, produces sulfide simultaneously；It is subsequently added mercury salt, consumes the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the mercury salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Hg performance improve simultaneously.
Its operating procedure is as follows:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.1-20%, solid content 0.5-25%, organic carbon content 0.5-20%, the mass ratio of organic carbon/sulfate (counting with SO4) is (1-50): 1, following reaction 0.1-5 days；
(2) in step (1) reacted solution, a certain amount of mercury salt, the mol ratio (1-50) of control sulfate/hydrargyrum: 1, the mass ratio (10-100) of organic carbon/hydrargyrum: 1, following reaction 0-5 days are added；
(3) repeat to add mercury salt 0-4 time in solution, the most all control the mol ratio (1-50) of sulfate/hydrargyrum: 1, the mass ratio (10-100) of organic carbon/hydrargyrum: 1, in 0-5 days response time, obtain efficient ecological reparation flora subsequently.
Comparing traditional mercurous agent, soil treatment method, this method has a following advantage:
1. the sulfate reduction flora cultivated itself is indigenous flora, and natural tolerance is strong, easily multiplies on a large scale in place, while effectively reduction Hg, does not results in ecological problem；
2. cultivating acclimation period significantly to reduce, can be trained the soonest in 1 day, economy is greatly improved；
3. while cultivating sulfate reducting bacteria, create metabolite sulfide, significantly alleviate the mercury salt added the subsequently toxicity to sulfate reducting bacteria, simultaneously because sulfate reducting bacteria is just had about suppression by metabolite sulfide itself, the most this method, while cultivating sulfate reducting bacteria rapidly, enhances its resistance to hydrargyrum characteristic；
4. hydrargyrum and sulfate reducting bacteria metabolite sulfide form cinnabar precipitation so that in soil, bivalent mercury is more stable, is significantly better than other biochemical process.
It is embodied as example as follows:
Embodiment 1:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.5%, solid content 10%, organic carbon content 12%, the mass ratio of organic carbon/sulfate is 5:1, following reaction 1 day；
(2) in step (1) reacted solution, a certain amount of mercury salt, the mol ratio (2-3) of control sulfate/hydrargyrum: 1, the mass ratio (10-30) of organic carbon/hydrargyrum: 1, following reaction 5 days are added；
(3) repeat in solution, to add hydrargyrum 2 times, the most all control the mol ratio (2-3) of sulfate/hydrargyrum: 1, the mass ratio (10-30) of organic carbon/hydrargyrum: 1, in 5 days response time, obtain efficient ecological subsequently and repair flora.
Embodiment 2:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 8-10%, solid content 10-15%, organic carbon content 10-15%, the mass ratio of organic carbon/sulfate is 20:1, following reaction 0.1-1 days；
(2) in step (1) reacted solution, a certain amount of mercury salt, the mol ratio (1-5) of control sulfate/hydrargyrum: 1, the mass ratio (30-80) of organic carbon/hydrargyrum: 1, following reaction 0-1 days are added；
(3) repeat in solution, to add hydrargyrum 1 time, the mol ratio (1-5) of control sulfate/hydrargyrum: 1, organic carbon/hydrargyrum mass ratio (30-80): 1,1 day response time, obtain efficient ecological subsequently and repair flora.
Above example is only in order to illustrate technical scheme, rather than is limited；Although the present invention being described in detail with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (4)
1. utilize biogas residue to prepare the method that Hg contaminated site efficient ecological repairs flora, it is characterized in that, by adding carbon source and sulfate in biogas residue, make sulfate reduction flora in biogas residue mushroom out, produce sulfide simultaneously；Being subsequently added mercury salt, consume the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the mercury salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Hg performance improve simultaneously, it comprises the steps of
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.1-20%, solid content 0.5-25%, organic carbon content 0.5-20%, the mass ratio of organic carbon/sulfate (counting with SO4) is (1-50): 1, following reaction 0.1-5 days；
(2) in step (1) reacted solution, a certain amount of mercury salt, the mol ratio (1-50) of control sulfate/hydrargyrum: 1, the mass ratio (10-100) of organic carbon/hydrargyrum: 1, following reaction 0-5 days are added；
(3) repeat to add mercury salt 0-4 time in solution, the most all control the mol ratio (1-50) of sulfate/hydrargyrum: 1, the mass ratio (10-100) of organic carbon/hydrargyrum: 1, in 0-5 days response time, obtain efficient ecological reparation flora subsequently.
A kind of utilize biogas residue prepare Hg contaminated site efficient ecological repair flora method, it is characterized in that, described biogas residue be agricultural, grocery trade debirs carry out the solid residue in dry type, half dry type and wet type anaerobic fermentation process.
A kind of method utilizing biogas residue to prepare Hg contaminated site efficient ecological reparation flora, it is characterized in that, described carbon source solution can be containing organic solution such as ethanol, saccharide, protein, starch, can also be for the solution containing the industry organic solid waste such as organic waste, changing food waste, it is also possible to for industrial and municipal organic wastewater.
A kind of method utilizing biogas residue to prepare Hg contaminated site efficient ecological reparation flora, it is characterized in that, described containing sulfate is the salt of containing sulfate radicals, and described mercury salt is the salt of mercury ion, it is possible to substituted by mercurous leached soil solutions or mercurous soil.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610370460.2A CN105969717A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration floras for Hg contaminated site from biogas residues |
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| CN201610370460.2A CN105969717A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration floras for Hg contaminated site from biogas residues |
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| CN105969717A true CN105969717A (en) | 2016-09-28 |
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| CN201610370460.2A Pending CN105969717A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration floras for Hg contaminated site from biogas residues |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1710082A (en) * | 2005-06-18 | 2005-12-21 | 山西大学 | Nano metal sulfide microbe preparing method |
| CN101402491A (en) * | 2008-10-27 | 2009-04-08 | 合肥工业大学 | Method for treating heavy metal-containing acidic waste water and recycling heavy metal with biomass fixed bed |
| CN101428930A (en) * | 2008-12-09 | 2009-05-13 | 中南大学 | Treatment process for hydrargyrum-containing flue gas washing wastewater from nonferrous metal smelting with biological agent |
| CN101705126A (en) * | 2009-07-28 | 2010-05-12 | 北京合百意可再生能源技术有限公司 | In-situ biogas coupling type long-acting desulfurizer |
| CN104230129A (en) * | 2014-09-01 | 2014-12-24 | 陈小洁 | Activated sludge treatment method |
-
2016
- 2016-05-30 CN CN201610370460.2A patent/CN105969717A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1710082A (en) * | 2005-06-18 | 2005-12-21 | 山西大学 | Nano metal sulfide microbe preparing method |
| CN101402491A (en) * | 2008-10-27 | 2009-04-08 | 合肥工业大学 | Method for treating heavy metal-containing acidic waste water and recycling heavy metal with biomass fixed bed |
| CN101428930A (en) * | 2008-12-09 | 2009-05-13 | 中南大学 | Treatment process for hydrargyrum-containing flue gas washing wastewater from nonferrous metal smelting with biological agent |
| CN101705126A (en) * | 2009-07-28 | 2010-05-12 | 北京合百意可再生能源技术有限公司 | In-situ biogas coupling type long-acting desulfurizer |
| CN104230129A (en) * | 2014-09-01 | 2014-12-24 | 陈小洁 | Activated sludge treatment method |
Non-Patent Citations (5)
| Title |
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| 冯紫艳: "硫酸盐还原菌和蓝藻对太湖底泥形成湖泛的模拟研究", 《中国博士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 夏大平等: "生物甲烷气实验的外加菌源（沼液）中厌氧菌群测定", 《高校地质学报》 * |
| 曹恒恒等: "重金属对硫酸盐还原菌影响", 《环境科学与技术》 * |
| 邢瑶: "沼渣沼液利用技术规程", 《现代农业》 * |
| 马春花等: "硫酸盐还原菌处理含重金属废水的实验研究", 《科技创新导报》 * |
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Application publication date: 20160928 |
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