CN105969715A - Method for preparing efficient ecological restoration floras for Cd contaminated site from sludge - Google Patents
Method for preparing efficient ecological restoration floras for Cd contaminated site from sludge Download PDFInfo
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- CN105969715A CN105969715A CN201610370418.0A CN201610370418A CN105969715A CN 105969715 A CN105969715 A CN 105969715A CN 201610370418 A CN201610370418 A CN 201610370418A CN 105969715 A CN105969715 A CN 105969715A
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- sulfate
- cadmium
- mud
- flora
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
Abstract
The invention provides a method for preparing efficient ecological restoration floras for a Cd contaminated site from sludge. A carbon source and sulfate are added into the sludge, sulfate reducing floras in the sludge are made to grow rapidly, and meanwhile sulfide is generated; next, cadmium salt is added to consume the sulfide inhibiting growth of sulfate reducing bacteria, the toxicity of the cadmium salt on the sulfate reducing bacteria is greatly lowered due to existence of the sulfide, and meanwhile Cd resistance of the cultured sulfate reducing floras is improved.
Description
Technical field
The present invention provides a kind of and utilizes mud to prepare the method that Cd contaminated site efficient ecological repairs flora, by adding carbon source and sulfate in mud, makes sulfate reduction flora in mud mushroom out, produces sulfide simultaneously；It is subsequently added cadmium salt, consumes the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the cadmium salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Cd performance improve simultaneously, belong to field of environment protection.
Background technology
Owing to mining, Metallurgical Waste Water arbitrarily discharge, cause a large amount of soil and polluted by Cd.Process the method containing cadmium soil at present the most defective.High-temperature melting method cost is high, and power consumption is big.Bioanalysis is long for reaction time, and easily causes the secondary pollution of adventive microbial inoculum.Solidification method effect is unstable, and soil is easily caused secondary pollution by firming agent.The patent No. 201510056406.6 describes a kind of curing containing heavy metal soil, but the method using substantial amounts of material, relatively costly, on the other hand solidification effect is unstable, the most easily there is carbonation in the calcium oxide as added so that alkaline-curing effect reduces.
Summary of the invention
For the deficiencies in the prior art, the present invention provides a kind of and utilizes mud to prepare the method that Cd contaminated site efficient ecological repairs flora, by adding carbon source and sulfate in mud, makes sulfate reduction flora in mud mushroom out, produces sulfide simultaneously；It is subsequently added cadmium salt, consumes the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the cadmium salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Cd performance improve simultaneously.
Its operating procedure is as follows:
(1) mud is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dewatered sludge content is 0.1-20%, solid content 0.5-25%, organic carbon content 0.5-20%, the mass ratio of organic carbon/sulfate (counting with SO4) is (1-50): 1, following reaction 0.1-5 days；
(2) in step (1) reacted solution, a certain amount of cadmium salt, the mol ratio (1-50) of control sulfate/cadmium salt: 1, the mass ratio (10-100) of organic carbon/cadmium: 1, following reaction 0-5 days are added；
(3) repeat to add cadmium salt 0-4 time in solution, the most all control the mol ratio (1-50) of sulfate/cadmium salt: 1, the mass ratio (10-100) of organic carbon/cadmium: 1, in 0-5 days response time, obtain efficient ecological reparation flora subsequently.
Comparing traditional containing cadmium agent, soil treatment method, this method has a following advantage:
1. the sulfate reduction flora cultivated itself is indigenous flora, and natural tolerance is strong, easily multiplies on a large scale in place, while effectively reduction Cd, does not results in ecological problem；
2. cultivating acclimation period significantly to reduce, can be trained the soonest in 1 day, economy is greatly improved；
3. while cultivating sulfate reducting bacteria, create metabolite sulfide, significantly alleviate the cadmium salt added the subsequently toxicity to sulfate reducting bacteria, simultaneously because sulfate reducting bacteria is just had about suppression by metabolite sulfide itself, the most this method, while cultivating sulfate reducting bacteria rapidly, enhances its resistance to cadmium characteristic；
4. bivalence cadmium forms sulfide-cadmium precipitation with sulfate reducting bacteria metabolite sulfide so that in soil, bivalence cadmium is more stable, is significantly better than other biochemical process.
It is embodied as example as follows:
Embodiment 1:
(1) mud is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dewatered sludge content is 0.5%, solid content 10%, organic carbon content 12%, the mass ratio of organic carbon/sulfate is 5:1, following reaction 1 day；
(2) in step (1) reacted solution, a certain amount of cadmium salt, the mol ratio (2-3) of control sulfate/cadmium: 1, the mass ratio (10-30) of organic carbon/cadmium: 1, following reaction 5 days are added；
(3) repeat in solution, to add cadmium salt 2 times, the most all control the mol ratio (2-3) of sulfate/cadmium: 1, the mass ratio (10-30) of organic carbon/cadmium: 1, in 5 days response time, obtain efficient ecological subsequently and repair flora.
Embodiment 2:
(1) mud is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dewatered sludge content is 8-10%, solid content 10-15%, organic carbon content 10-15%, the mass ratio of organic carbon/sulfate is 20:1, following reaction 0.1-1 days；
(2) in step (1) reacted solution, a certain amount of cadmium salt, the mol ratio (1-5) of control sulfate/cadmium: 1, the mass ratio (30-80) of organic carbon/cadmium: 1, following reaction 0-1 days are added；
(3) repeat in solution, to add cadmium salt 1 time, the mol ratio (1-5) of control sulfate/cadmium: 1, the mass ratio (30-80) of organic carbon/cadmium: 1,1 day response time, obtain efficient ecological subsequently and repair flora.
Above example is only in order to illustrate technical scheme, rather than is limited；Although the present invention being described in detail with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (4)
1. utilize mud to prepare the method that Cd contaminated site efficient ecological repairs flora, it is characterized in that, by adding carbon source and sulfate in mud, make sulfate reduction flora in mud mushroom out, produce sulfide simultaneously；Being subsequently added cadmium salt, consume the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the cadmium salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Cd performance improve simultaneously, it comprises the steps of
(1) mud is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dewatered sludge content is 0.1-20%, solid content 0.5-25%, organic carbon content 0.5-20%, the mass ratio of organic carbon/sulfate (counting with SO4) is (1-50): 1, following reaction 0.1-5 days；
(2) in step (1) reacted solution, a certain amount of cadmium salt, the mol ratio (1-50) of control sulfate/cadmium: 1, the mass ratio (10-100) of organic carbon/cadmium: 1, following reaction 0-5 days are added；
(3) repeat to add cadmium salt 0-4 time in solution, the most all control the mol ratio (1-50) of sulfate/cadmium: 1, the mass ratio (10-100) of organic carbon/cadmium: 1, in 0-5 days response time, obtain efficient ecological reparation flora subsequently.
A kind of method utilizing mud to prepare Cd contaminated site efficient ecological reparation flora, is characterized in that, described mud is anaerobic sludge produced by sewage treatment anaerobic technique, it is possible to the double oxygen mud of admixture part.
A kind of method utilizing mud to prepare Cd contaminated site efficient ecological reparation flora, it is characterized in that, described carbon source solution can be containing organic solution such as ethanol, saccharide, protein, starch, can also be for the solution containing the industry organic solid waste such as organic waste, changing food waste, it is also possible to for industrial and municipal organic wastewater.
A kind of method utilizing mud to prepare Cd contaminated site efficient ecological reparation flora, it is characterized in that, described containing sulfate is the salt of containing sulfate radicals, and described cadmium salt is the salt containing cadmium ion, it is possible to substituted containing cadmium leached soil solutions or the soil Han cadmium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610370418.0A CN105969715A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration floras for Cd contaminated site from sludge |
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| CN201610370418.0A CN105969715A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration floras for Cd contaminated site from sludge |
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| CN201610370418.0A Pending CN105969715A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration floras for Cd contaminated site from sludge |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107138521A (en) * | 2017-05-08 | 2017-09-08 | 北京航空航天大学 | A kind of repairing method of microorganism of cadmium pollution bed mud |
| CN110078209A (en) * | 2019-05-16 | 2019-08-02 | 桂林电子科技大学 | A kind of method of micro-reduction sulfate formation cadmium sulphur mine |
| CN110093275A (en) * | 2019-05-14 | 2019-08-06 | 桂林电子科技大学 | A kind of acclimation method of resistance to cadmium sulfate-reducing activity sludge |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210503A (en) * | 1996-02-06 | 1999-03-10 | 蒂奥帕克硫系统公司 | Treatment of water containing heavy metal ions |
| CN103562146A (en) * | 2011-06-10 | 2014-02-05 | 孟加拉邦科技大学 | Self-sustained microbial detoxification of soluble sulfate from environmental effluent |
| CN103981097A (en) * | 2013-12-05 | 2014-08-13 | 青岛理工大学 | Preparation method of remediation flora for Cr (VI) contaminated site from sludge |
-
2016
- 2016-05-30 CN CN201610370418.0A patent/CN105969715A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1210503A (en) * | 1996-02-06 | 1999-03-10 | 蒂奥帕克硫系统公司 | Treatment of water containing heavy metal ions |
| CN103562146A (en) * | 2011-06-10 | 2014-02-05 | 孟加拉邦科技大学 | Self-sustained microbial detoxification of soluble sulfate from environmental effluent |
| CN103981097A (en) * | 2013-12-05 | 2014-08-13 | 青岛理工大学 | Preparation method of remediation flora for Cr (VI) contaminated site from sludge |
Non-Patent Citations (2)
| Title |
|---|
| 范文宏等: "硫酸盐还原菌修复污染土壤过程中镉的地球化学形态分布变化", 《环境科学学报》 * |
| 马春花等: "硫酸盐还原菌处理含重金属废水的实验研究", 《科技创新导报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107138521A (en) * | 2017-05-08 | 2017-09-08 | 北京航空航天大学 | A kind of repairing method of microorganism of cadmium pollution bed mud |
| CN110093275A (en) * | 2019-05-14 | 2019-08-06 | 桂林电子科技大学 | A kind of acclimation method of resistance to cadmium sulfate-reducing activity sludge |
| CN110078209A (en) * | 2019-05-16 | 2019-08-02 | 桂林电子科技大学 | A kind of method of micro-reduction sulfate formation cadmium sulphur mine |
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Application publication date: 20160928 |
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