CN105950537A - Method for preparing efficient ecological restoration flora for Cd-polluted site by utilizing biogas residue - Google Patents
Method for preparing efficient ecological restoration flora for Cd-polluted site by utilizing biogas residue Download PDFInfo
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- CN105950537A CN105950537A CN201610370471.0A CN201610370471A CN105950537A CN 105950537 A CN105950537 A CN 105950537A CN 201610370471 A CN201610370471 A CN 201610370471A CN 105950537 A CN105950537 A CN 105950537A
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- sulfate
- cadmium
- flora
- biogas residue
- salt
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- 238000000034 method Methods 0.000 title claims abstract description 19
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims abstract description 41
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 25
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 25
- 150000001661 cadmium Chemical class 0.000 claims abstract description 18
- 241000894006 Bacteria Species 0.000 claims abstract description 13
- 230000001988 toxicity Effects 0.000 claims abstract description 5
- 231100000419 toxicity Toxicity 0.000 claims abstract description 5
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 4
- 229910052793 cadmium Inorganic materials 0.000 claims description 21
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims description 21
- 239000000243 solution Substances 0.000 claims description 18
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 claims description 12
- 230000009467 reduction Effects 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 8
- 230000008439 repair process Effects 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 5
- 238000005842 biochemical reaction Methods 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 230000004044 response Effects 0.000 claims description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 3
- 239000002351 wastewater Substances 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 229920002472 Starch Polymers 0.000 claims 1
- WLZRMCYVCSSEQC-UHFFFAOYSA-N cadmium(2+) Chemical compound [Cd+2] WLZRMCYVCSSEQC-UHFFFAOYSA-N 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 239000010794 food waste Substances 0.000 claims 1
- 239000010815 organic waste Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000002910 solid waste Substances 0.000 claims 1
- 235000019698 starch Nutrition 0.000 claims 1
- 239000008107 starch Substances 0.000 claims 1
- 150000003568 thioethers Chemical class 0.000 abstract 3
- 230000000694 effects Effects 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 1
- 238000011953 bioanalysis Methods 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Environmental & Geological Engineering (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Soil Sciences (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for preparing an efficient ecological restoration flora for a Cd-polluted site by utilizing biogas residue. According to the method, by adding a carbon source and sulfate into the biogas residue, a sulfate reducing flora in the biogas residue rapidly grows, and sulfides are generated at the same time; then, cadmium salt is added into the biogas residue, and the sulfides capable of inhibiting the growth of the sulfate reducing bacteria are consumed; due to the existence of the sulfides, the toxicity of the cadmium salt to the sulfate reducing bacteria is greatly reduced, and the Cd resistance of the cultured sulfate reducing flora is also improved.
Description
Technical field
The present invention provides a kind of and utilizes biogas residue to prepare the method that Cd contaminated site efficient ecological repairs flora, by adding carbon source and sulfate in biogas residue, makes sulfate reduction flora in biogas residue mushroom out, produces sulfide simultaneously；It is subsequently added cadmium salt, consumes the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the cadmium salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Cd performance improve simultaneously, belong to field of environment protection.
Background technology
Owing to mining, Metallurgical Waste Water arbitrarily discharge, cause a large amount of soil and polluted by Cd.Process the method containing cadmium soil at present the most defective.High-temperature melting method cost is high, and power consumption is big.Bioanalysis is long for reaction time, and easily causes the secondary pollution of adventive microbial inoculum.Solidification method effect is unstable, and soil is easily caused secondary pollution by firming agent.The patent No. 201510056406.6 describes a kind of curing containing heavy metal soil, but the method using substantial amounts of material, relatively costly, on the other hand solidification effect is unstable, the most easily there is carbonation in the calcium oxide as added so that alkaline-curing effect reduces.
Summary of the invention
For the deficiencies in the prior art, the present invention provides a kind of and utilizes biogas residue to prepare the method that Cd contaminated site efficient ecological repairs flora, by adding carbon source and sulfate in biogas residue, makes sulfate reduction flora in biogas residue mushroom out, produces sulfide simultaneously；It is subsequently added cadmium salt, consumes the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the cadmium salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Cd performance improve simultaneously.
Its operating procedure is as follows:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.1-20%, solid content 0.5-25%, organic carbon content 0.5-20%, the mass ratio of organic carbon/sulfate (counting with SO4) is (1-50): 1, following reaction 0.1-5 days；
(2) in step (1) reacted solution, a certain amount of cadmium salt, the mol ratio (1-50) of control sulfate/cadmium salt: 1, the mass ratio (10-100) of organic carbon/cadmium: 1, following reaction 0-5 days are added；
(3) repeat to add cadmium salt 0-4 time in solution, the most all control the mol ratio (1-50) of sulfate/cadmium salt: 1, the mass ratio (10-100) of organic carbon/cadmium: 1, in 0-5 days response time, obtain efficient ecological reparation flora subsequently.
Comparing traditional containing cadmium agent, soil treatment method, this method has a following advantage:
1. the sulfate reduction flora cultivated itself is indigenous flora, and natural tolerance is strong, easily multiplies on a large scale in place, while effectively reduction Cd, does not results in ecological problem；
2. cultivating acclimation period significantly to reduce, can be trained the soonest in 1 day, economy is greatly improved；
3. while cultivating sulfate reducting bacteria, create metabolite sulfide, significantly alleviate the cadmium salt added the subsequently toxicity to sulfate reducting bacteria, simultaneously because sulfate reducting bacteria is just had about suppression by metabolite sulfide itself, the most this method, while cultivating sulfate reducting bacteria rapidly, enhances its resistance to cadmium characteristic；
4. bivalence cadmium forms sulfide-cadmium precipitation with sulfate reducting bacteria metabolite sulfide so that in soil, bivalence cadmium is more stable, is significantly better than other biochemical process.
It is embodied as example as follows:
Embodiment 1:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.5%, solid content 10%, organic carbon content 12%, the mass ratio of organic carbon/sulfate is 5:1, following reaction 1 day；
(2) in step (1) reacted solution, a certain amount of cadmium salt, the mol ratio (2-3) of control sulfate/cadmium: 1, the mass ratio (10-30) of organic carbon/cadmium: 1, following reaction 5 days are added；
(3) repeat in solution, to add cadmium salt 2 times, the most all control the mol ratio (2-3) of sulfate/cadmium: 1, the mass ratio (10-30) of organic carbon/cadmium: 1, in 5 days response time, obtain efficient ecological subsequently and repair flora.
Embodiment 2:
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 8-10%, solid content 10-15%, organic carbon content 10-15%, the mass ratio of organic carbon/sulfate is 20:1, following reaction 0.1-1 days；
(2) in step (1) reacted solution, a certain amount of cadmium salt, the mol ratio (1-5) of control sulfate/cadmium: 1, the mass ratio (30-80) of organic carbon/cadmium: 1, following reaction 0-1 days are added；
(3) repeat in solution, to add cadmium salt 1 time, the mol ratio (1-5) of control sulfate/cadmium: 1, the mass ratio (30-80) of organic carbon/cadmium: 1,1 day response time, obtain efficient ecological subsequently and repair flora.
Above example is only in order to illustrate technical scheme, rather than is limited；Although the present invention being described in detail with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or wherein portion of techniques feature is carried out equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (4)
1. utilize biogas residue to prepare the method that Cd contaminated site efficient ecological repairs flora, it is characterized in that, by adding carbon source and sulfate in biogas residue, make sulfate reduction flora in biogas residue mushroom out, produce sulfide simultaneously；Being subsequently added cadmium salt, consume the sulfide of inhibiting sulfate reduction bacteria growing, and the existence of sulfide has been greatly reduced the cadmium salt toxicity to sulfate reducting bacteria, makes cultivated sulfate reduction flora resistance to Cd performance improve simultaneously, it comprises the steps of
(1) biogas residue is mixed with the solution containing a certain amount of carbon source, add a certain amount of sulfate, put into and reactor carries out biochemical reaction, controlling mixed solution dried biogas residues content is 0.1-20%, solid content 0.5-25%, organic carbon content 0.5-20%, the mass ratio of organic carbon/sulfate (counting with SO4) is (1-50): 1, following reaction 0.1-5 days；
(2) in step (1) reacted solution, a certain amount of cadmium salt, the mol ratio (1-50) of control sulfate/cadmium: 1, the mass ratio (10-100) of organic carbon/cadmium: 1, following reaction 0-5 days are added；
(3) repeat to add cadmium salt 0-4 time in solution, the most all control the mol ratio (1-50) of sulfate/cadmium: 1, the mass ratio (10-100) of organic carbon/cadmium: 1, in 0-5 days response time, obtain efficient ecological reparation flora subsequently.
A kind of utilize biogas residue prepare Cd contaminated site efficient ecological repair flora method, it is characterized in that described biogas residue be agricultural, grocery trade debirs carry out the solid residue in dry type, half dry type and wet type anaerobic fermentation process.
A kind of method utilizing biogas residue to prepare Cd contaminated site efficient ecological reparation flora, it is characterized in that, described carbon source solution can be containing organic solution such as ethanol, saccharide, protein, starch, can also be for the solution containing the industry organic solid waste such as organic waste, changing food waste, it is also possible to for industrial and municipal organic wastewater.
A kind of method utilizing biogas residue to prepare Cd contaminated site efficient ecological reparation flora, it is characterized in that, described containing sulfate is the salt of containing sulfate radicals, and described cadmium salt is the salt containing cadmium ion, it is possible to substituted containing cadmium leached soil solutions or the soil Han cadmium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610370471.0A CN105950537A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration flora for Cd-polluted site by utilizing biogas residue |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610370471.0A CN105950537A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration flora for Cd-polluted site by utilizing biogas residue |
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| CN105950537A true CN105950537A (en) | 2016-09-21 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610370471.0A Pending CN105950537A (en) | 2016-05-30 | 2016-05-30 | Method for preparing efficient ecological restoration flora for Cd-polluted site by utilizing biogas residue |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018233613A1 (en) * | 2017-06-20 | 2018-12-27 | 青岛理工大学 | Method for processing waste incineration fly ash by using biogas residues |
| CN110093275A (en) * | 2019-05-14 | 2019-08-06 | 桂林电子科技大学 | A kind of acclimation method of resistance to cadmium sulfate-reducing activity sludge |
Citations (3)
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|---|---|---|---|---|
| CN103193316A (en) * | 2013-04-15 | 2013-07-10 | 北京工业大学 | Method for biologically treating cadmium-containing wastewater |
| CN104450552A (en) * | 2014-08-17 | 2015-03-25 | 西北大学 | Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil |
| CN105441361A (en) * | 2015-12-29 | 2016-03-30 | 中国电建集团中南勘测设计研究院有限公司 | Method for preparing strain and microbial agent for heavy metal contaminated water treatment |
-
2016
- 2016-05-30 CN CN201610370471.0A patent/CN105950537A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103193316A (en) * | 2013-04-15 | 2013-07-10 | 北京工业大学 | Method for biologically treating cadmium-containing wastewater |
| CN104450552A (en) * | 2014-08-17 | 2015-03-25 | 西北大学 | Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil |
| CN105441361A (en) * | 2015-12-29 | 2016-03-30 | 中国电建集团中南勘测设计研究院有限公司 | Method for preparing strain and microbial agent for heavy metal contaminated water treatment |
Non-Patent Citations (2)
| Title |
|---|
| 周冰玉等: "高耐镉硫酸盐还原菌的筛选及鉴定", 《湖南农业科学》 * |
| 范文宏等: "硫酸盐还原菌修复污染土壤过程中镉的地球化学形态分布变化", 《环境科学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018233613A1 (en) * | 2017-06-20 | 2018-12-27 | 青岛理工大学 | Method for processing waste incineration fly ash by using biogas residues |
| CN110093275A (en) * | 2019-05-14 | 2019-08-06 | 桂林电子科技大学 | A kind of acclimation method of resistance to cadmium sulfate-reducing activity sludge |
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Application publication date: 20160921 |
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