CN106009767A - Method for extracting melanin from Taihe black-bone silky fowl - Google Patents
Method for extracting melanin from Taihe black-bone silky fowl Download PDFInfo
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- CN106009767A CN106009767A CN201610403222.7A CN201610403222A CN106009767A CN 106009767 A CN106009767 A CN 106009767A CN 201610403222 A CN201610403222 A CN 201610403222A CN 106009767 A CN106009767 A CN 106009767A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
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Abstract
The invention relates to a method for extracting melanin from a Taihe black-bone silky fowl, which belongs to the field of advanced processing of animals and rare birds. The method is characterized by comprising the following steps: variety selection, slaughtering and cleaning, crushing, grinding with a colloid mill, homogenization, double-enzyme hydrolysis, standing for layering after enzyme deactivation, centrifugation and removal of supernatant so as to obtain crude melanin, extraction and separation with a strong alkali solution so as to obtain melanin, and drying and packaging. In the step of double-enzyme hydrolysis, both papain and flavorzyme are used for enzymatic hydrolysis at the same time, and the crude melanin is obtained after enzymatic hydrolysis; and the strong alkali solution is a sodium hydroxide solution, filtering is carried out after extraction with stirring for 1.5 h, and insoluble substances are repeatedly extracted four times so as to obtain the melanin. The method provided by the invention employs a double-enzyme extraction process; under the action of the two enzymes, the components of the cell walls of the Taihe black-bone silky fowl are hydrolyzed, original cell wall structures are destroyed, and effective components of the Taihe black-bone silky fowl are dissolved or suspended in a solvent; thus, extraction of the effective components are benefited, the form and chemical structure of the melanin of the Taihe black-bone silky fowl are prevented from destroy, and the quality of the melanin is guaranteed.
Description
Technical field
The present invention relates to animal rare bird class field of deep, from Taihe chicken, extract melanic method particularly to one.
Background technology
Taihe chicken is Taihe County, Jiangxi Province special product, because of have Cong Guan, tassel head, green ear, beard, silk hair, dry foot, five pawls,
Crow skin, crow meat, the crow big feature of bone ten and high nutritive value and medical value and world-famous.Just took at bar as far back as 1915
It is cited as " viewing and admiring chicken kind " in the world exposition of the horse Pacific Ocean, is awarded a gold medal.Within 2000, calm and peaceful Gallus Domesticus is set to head by the Ministry of Agriculture
Criticize national poultry protection kind；Within 2002, calm and peaceful dark egg has carried out the examination of space life sciences in " divine boat three " spacecraft
Test；Within 2004, calm and peaceful Gallus Domesticus becomes geographical indication protection product；Participate in universal geography mark conference exhibition in June, 2007 and list in
Universal geography famous special product register, is protected by World Intellectual Property Organization.In June, 2007 People's Republic of China's standard GB/T/T
In 21004-2007 ground, the calm and peaceful Gallus Domesticus of famous special product is formally promulgated and enforcement in December 1 day.Chinese medicine dictionary and " Ci hai "
In, all title Taihe chicken are the Plant's kind that China is precious, the effect that having is used as medicine cures the disease；" Medical Animals In China will " also
Describe: Taihe chicken has the function of invigorating the liver and kidney, replenishing QI and blood, clearind deficient heat, cures mainly seminal emission, chronic diarrhea chronic dysentery, quenches one's thirst, red in vain
Leukorrhagia, hectic fever due to YIN-deficiency consumptive fever etc.." WUJI BAIFENG WAN " with Taihe chicken as raw material production is well-known, and Taihe chicken is cured the disease
It is high that proved recipe and curative effect make medical circle chase after especially.
Melanin in Gallus Domesticus belongs to eumelanin, and melanin has effect of protection health, can avoid ultraviolet radiation
The acute and chronic diseases caused, is uniquely to protect organisms from width to shoot harmful native endogenous biopolymer wounded.Melanin can have
Effect scavenging hydroxyl, scavenging capacity oxygen-derived free radicals, removing DPPH.free radical, melanin is to trace element
There is enrichment, biological existence, the ability grown, grow, unexpectedly strive can be improved, have simultaneously prophylaxis of cancer, defying age,
Improve effect of immunity.Calm and peaceful Gallus Domesticus is rich in melanin, and its skin, meat, bone, eyes are black, especially these positions
Melanin content is the highest, and in the deep processing of Gallus Domesticus product, extracting and separate melanin is to reduce production cost, improves economy effect
The effective ways of benefit.
Summary of the invention
It is an object of the invention to a kind of from Taihe chicken, extract melanic method, select science, process technology fine,
High in technological content, it is ensured that the high-quality of product.
The technical problem of the present invention is mainly addressed by following technical proposals:
One extracts melanic method from Taihe chicken, it is characterised in that include following technological process: choose seeds, slaughter
Stratification after cleaning, pulverizing, colloid mill, homogenizing, double-enzyme hydrolysis, enzyme denaturing, centrifugal segregation supernatant, crude melanin,
Strong base solution extraction separates, obtains melanin, dry packing.
Concrete, described double-enzyme hydrolysis uses papain and flavor protease enzymolysis simultaneously, obtains crude melanin after enzymolysis.Institute
Stating strong base solution is sodium hydroxide solution, and stirring is filtered after extracting 1～1.5 hour, and insoluble matter repeats to extract 4～6 times to obtain black
Element.
As preferably, after described melanin extracts, residual components prepares black-bone chicken paste, more spray-dried system by Maillard reaction
Standby Gallus Domesticus powder.
Specifically include two steps:
1. dual-enzyme hydrolysis method extraction melanin of black-bone chicken:
With Taihe chicken through slaughtering, dehematizing, clean after hair, internal organs (chicken, killed or stripped feather off) as raw material, bone mud crusher machine, add clean
Chicken weighs the purified water defibrination of 3 times, and Gallus Domesticus was starched colloid mill, and Task-size Controlling is below 10 μm, and honed Gallus Domesticus liquid is the most equal
Matter machine, controls second voltage regulation valve rated pressure and reaches the standard grade 20Mpa, and one-level pressure regulator valve rated pressure is reached the standard grade 60Mpa, adjusts crow after homogenizing
Bone chicken Carnis Gallus domesticus slurry pH value is 5.5～6.0, controls temperature under the conditions of 50～55 DEG C, heavily add by chicken papain 0.09Au%,
Flavor protease 120LAPu%, keeps 50～55 DEG C, 3～4 hours carrying out enzymolysis under agitation, and enzymolysis heats up after terminating
To 95 DEG C, react 30 minutes enzyme denaturing.Stratification after enzyme denaturing, extracts upper strata suspension out, under the conditions of 4800r/min with from
Scheming is centrifuged 15min, abandoning supernatant, obtains Gallus Domesticus melanin crude product.
The most melanic separation and refined:
Extract containing substantial amounts of protein in the Gallus Domesticus melanin crude product obtained, and Gallus Domesticus melanin be tight with protein bound,
Therefore the melanic separation key of Gallus Domesticus is deproteination matter, and the protein major part in melanin of black-bone chicken crude product is alkalescence egg
White matter, available strong base solution carries out washing and removes.Its method is:
Being mixed in the sodium hydroxide solution that pH value is 10 by Gallus Domesticus melanin crude product, solid-liquid ratio is 1:10, controls temperature and is
60 DEG C, stirring is filtered after extracting washing 1.5 hours, and it is the sodium hydroxide solution of 10 that insoluble matter adds 10 times amount pH values again, 60 DEG C
Washing 1.5 hours is extracted in stirring, by that analogy, is repeated 4 times to obtain melanin of black-bone chicken.The melanin of black-bone chicken drying and packaging that will make
?.
The medicine have the advantages that
1, technique of the present invention is double enzyme extraction techniques, and Gallus Domesticus is the constituent (ammonia of its cell wall under the effect of double enzymes
Base acid, protein, melanin, cellulose, hemicellulose etc.) it is hydrolyzed, destroy archeocyte wall construction, make the effective of Gallus Domesticus
Composition dissolves or is suspended in solvent, the beneficially extraction of effective ingredient.
2, shorten the time of enzymolysis than single enzymolysis technique, saved enzyme dosage, reduce production cost.
3, dual-enzyme hydrolysis method can guarantee that the form of melanin of black-bone chicken and chemical constitution will not be destroyed, it is ensured that melanic quality.
4, this method technique is simple, equipment investment is few, with short production cycle, with low cost.
Detailed description of the invention
Below by embodiment, technical scheme is described in further detail.
Embodiment 1:
With Taihe chicken through slaughtering, dehematizing, clean after hair, internal organs (chicken, killed or stripped feather off) as raw material, bone mud crusher machine, add clean
Chicken weighs the purified water defibrination of 3 times, and Gallus Domesticus was starched colloid mill, and Task-size Controlling is below 10 μm, and honed Gallus Domesticus liquid is the most equal
Matter machine, controls second voltage regulation valve rated pressure and reaches the standard grade 20Mpa, and one-level pressure regulator valve rated pressure is reached the standard grade 60Mpa, adjusts crow after homogenizing
Bone chicken Carnis Gallus domesticus slurry pH value be 5.5 control temperature under the conditions of 50 DEG C, heavily add papain 0.09Au%, local flavor egg by chicken
White enzyme 120LAPu%, keeps 50 DEG C, 3～4 hours carrying out enzymolysis under agitation, and enzymolysis is warming up to 95 DEG C after terminating, instead
Answer 30 minutes enzyme denaturing.Stratification after enzyme denaturing, extracts upper strata suspension out, uses centrifuge 15min under the conditions of 4800r/min,
Abandoning supernatant, obtains Gallus Domesticus melanin crude product.
Being mixed in the sodium hydroxide solution that pH value is 10 by Gallus Domesticus melanin crude product, solid-liquid ratio is 1:10, controls temperature and is
60 DEG C, stirring is filtered after extracting washing 1.5 hours, and it is the sodium hydroxide solution of 10 that insoluble matter adds 10 times amount pH values again, 60 DEG C
Washing 1.5 hours is extracted in stirring, by that analogy, is repeated 4 times to obtain melanin of black-bone chicken.The melanin of black-bone chicken drying and packaging that will make
?.
Embodiment 2:
With Taihe chicken through slaughtering, dehematizing, clean after hair, internal organs (chicken, killed or stripped feather off) as raw material, bone mud crusher machine, add clean
Chicken weighs the purified water defibrination of 3 times, and Gallus Domesticus was starched colloid mill, and Task-size Controlling is below 10 μm, and honed Gallus Domesticus liquid is the most equal
Matter machine, controls second voltage regulation valve rated pressure and reaches the standard grade 20Mpa, and one-level pressure regulator valve rated pressure is reached the standard grade 60Mpa, adjusts crow after homogenizing
Bone chicken Carnis Gallus domesticus slurry pH value is 6, controls temperature under the conditions of 55 DEG C, heavily adds papain 0.09Au%, local flavor egg by chicken
White enzyme 120LAPu%, keeps 55 DEG C, 3～4 hours carrying out enzymolysis under agitation, and enzymolysis is warming up to 95 DEG C after terminating, instead
Answer 30 minutes enzyme denaturing.Stratification after enzyme denaturing, extracts upper strata suspension out, uses centrifuge 15min under the conditions of 4800r/min,
Abandoning supernatant, obtains Gallus Domesticus melanin crude product.
Being mixed in the aqua calcis that pH value is 10 by Gallus Domesticus melanin crude product, solid-liquid ratio is 1:10, controls temperature and is
60 DEG C, stirring is filtered after extracting washing 1 hour, and it is the aqua calcis of 10 that insoluble matter adds 10 times amount pH values again, and 60 DEG C are stirred
Mix extraction washing 1 hour, by that analogy, be repeated 5 times to obtain melanin of black-bone chicken.By the melanin of black-bone chicken drying and packaging made.
Example executes 3:
With Taihe chicken through slaughtering, dehematizing, clean after hair, internal organs (chicken, killed or stripped feather off) as raw material, bone mud crusher machine, add clean
Chicken weighs the purified water defibrination of 3 times, and Gallus Domesticus was starched colloid mill, and Task-size Controlling is below 10 μm, and honed Gallus Domesticus liquid is the most equal
Matter machine, controls second voltage regulation valve rated pressure and reaches the standard grade 20Mpa, and one-level pressure regulator valve rated pressure is reached the standard grade 60Mpa, adjusts crow after homogenizing
Bone chicken Carnis Gallus domesticus slurry pH value is 6.0, controls temperature under the conditions of 55 DEG C, heavily adds papain 0.09Au%, local flavor by chicken
Protease 120LAPu%, keeps 55 DEG C, 3～4 hours carrying out enzymolysis under agitation, and enzymolysis is warming up to 95 DEG C after terminating,
React 30 minutes enzyme denaturing.Stratification after enzyme denaturing, extracts upper strata suspension out, uses centrifuge under the conditions of 4800r/min
15min, abandoning supernatant, obtain Gallus Domesticus melanin crude product.
Being mixed in the sodium hydroxide solution that pH value is 10 by Gallus Domesticus melanin crude product, solid-liquid ratio is 1:10, controls temperature and is
60 DEG C, stirring is filtered after extracting washing 1.5 hours, and it is the sodium hydroxide solution of 10 that insoluble matter adds 10 times amount pH values again, 60 DEG C
Washing 1.5 hours is extracted in stirring, by that analogy, is repeated 4 times to obtain melanin of black-bone chicken.The melanin of black-bone chicken drying and packaging that will make
?.
The present embodiment is the embodiment of example of the present invention, for those skilled in the art, the invention discloses
On the basis of application process and principle, it is easy to make various types of improvement, it is not limited solely to the above-mentioned specific embodiment party of the present invention
Method described by formula, structure, technique, the most previously described mode is preferred version, and the most restrictive meaning
Justice, every equivalence change made according to the present invention and amendment, all in the scope protection domain of claims of the present invention.
Claims (5)
1. one kind is extracted melanic method from Taihe chicken, it is characterised in that include following technological process: choose seeds, kill
Kill stratification after cleaning, pulverizing, colloid mill, homogenizing, double-enzyme hydrolysis, enzyme denaturing, centrifugal segregation supernatant, crude melanin,
Strong base solution extraction separates, obtains melanin, dry packing.
The most according to claim 1 from Taihe chicken, extract melanic method, it is characterised in that described pair of enzyme water
Solving and use papain and flavor protease enzymolysis simultaneously, enzyme dosage for heavily being added papain 0.09Au%, local flavor by chicken
Protease 120LAPu%, hydrolysis temperature is 50～55 DEG C, and enzymolysis time is 3～4 hours, obtains crude melanin after enzymolysis.
The most according to claim 1 from Taihe chicken, extract melanic method, it is characterised in that described highly basic is molten
Liquid is sodium hydroxide solution, and solid-liquid ratio is 1:10, and pH value is 10, and temperature is 60 DEG C, and stirring is filtered after extracting 1.5 hours,
Insoluble matter repeats to extract 4 times to obtain melanin.
The most according to claim 1 from Taihe chicken, extract melanic method, it is characterised in that described colloid mill
In operation, chicken weight is 1:3 with the ratio of water.
The most according to claim 1 from Taihe chicken, extract melanic method, it is characterised in that described melanin
After extraction, residual components prepares black-bone chicken paste by Maillard reaction, more spray-dried prepares Gallus Domesticus powder.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610403222.7A CN106009767A (en) | 2016-06-11 | 2016-06-11 | Method for extracting melanin from Taihe black-bone silky fowl |
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| CN201610403222.7A CN106009767A (en) | 2016-06-11 | 2016-06-11 | Method for extracting melanin from Taihe black-bone silky fowl |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107523602A (en) * | 2017-10-14 | 2017-12-29 | 江西倍得力生物工程有限公司 | A kind of extracting method of black-bone chicken active peptide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1121519A (en) * | 1994-10-25 | 1996-05-01 | 纪宝臣 | Food melanin extracting method |
| CN101058821A (en) * | 2007-05-11 | 2007-10-24 | 华南理工大学 | Method for extracting black-bone chicken crude melanin |
| CN101157804A (en) * | 2006-12-28 | 2008-04-09 | 中国农业大学 | Method for extracting melanin from black-bone chicken |
-
2016
- 2016-06-11 CN CN201610403222.7A patent/CN106009767A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1121519A (en) * | 1994-10-25 | 1996-05-01 | 纪宝臣 | Food melanin extracting method |
| CN101157804A (en) * | 2006-12-28 | 2008-04-09 | 中国农业大学 | Method for extracting melanin from black-bone chicken |
| CN101058821A (en) * | 2007-05-11 | 2007-10-24 | 华南理工大学 | Method for extracting black-bone chicken crude melanin |
Non-Patent Citations (2)
| Title |
|---|
| 李欣 等: "乌骨鸡黑色素的提取分离及抗氧化活性研究", 《食品科学》 * |
| 杨瑞学 等: "乌骨鸡深加工酶解处理条件的探索研究", 《食品科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107523602A (en) * | 2017-10-14 | 2017-12-29 | 江西倍得力生物工程有限公司 | A kind of extracting method of black-bone chicken active peptide |
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Application publication date: 20161012 |