CN101058821A - Method for extracting black-bone chicken crude melanin - Google Patents
Method for extracting black-bone chicken crude melanin Download PDFInfo
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- CN101058821A CN101058821A CN 200710027969 CN200710027969A CN101058821A CN 101058821 A CN101058821 A CN 101058821A CN 200710027969 CN200710027969 CN 200710027969 CN 200710027969 A CN200710027969 A CN 200710027969A CN 101058821 A CN101058821 A CN 101058821A
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- bone chicken
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- melanin
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract
The invention discloses an extracting method of black-bone chicken heavy melanin, which comprises the following steps: enzymolyzing black-bone chicken in the proper temperature and proper pH conditions with protease; centrifugalizing the enzymolysis liquid; getting black-bone chicken heavy melanin after drying. The method shortens the time of enzymolysis, which provides a simple method, a good economic profit and a good application prospect.
Description
Technical field
The present invention relates to melanic extractive technique field, specifically be meant a kind of extracting method of black-bone chicken crude melanin.
Background technology
Black-bone chicken is rich in melanochrome, and melanochrome contains the amino acid of needed by human etc., can improve intelligence, and hypermnesis prevents anaemia and trichomadesis, regulates physiological function.Discovering in recent years, melanin of black-bone chicken have the free radical of removing and antioxygenation, prevent cell aging, the effect of enhancing body immunizing power.For the extracting method of melanin of black-bone chicken, domestic have some correlative studys.Extracting method includes hydrochloric acid reflux method and enzymolysis process.The hydrochloric acid reflux method, the hydrochloric acid reflux overlong time, about 50 hours, it was too big to consume energy, and the blackbone Chicken part all can not be utilized again; The research that enzymolysis process extracts melanin of black-bone chicken is found in " meat industry " 2005 12 phases, and selecting the calm and peaceful Gallus Domesticus in Jiangxi for use is research object, adopts acid protease hydrolysis protein separation melanin of black-bone chicken, enzyme concentration 3.8%, meat water is than 1: 2, enzymatic hydrolysis condition pH2.8,34.8 ℃ of hydrolysis temperatures, enzymolysis time 20h, compare with the hydrochloric acid reflux method, the time has shortened some, but reaches 20 hours enzymolysis time, exist energy consumption and efficiency equally, and be difficult to solve anticorrosion problem.
Summary of the invention
The objective of the invention is deficiency, a kind of extracting method of black-bone chicken crude melanin is provided at present technology.
Technical scheme of the present invention provides a kind of extracting method of black-bone chicken crude melanin, may further comprise the steps:
(1) black-bone chicken hair chicken is rubbed into meat gruel then through butchering unhairing, removing internal organ, adds 1-2 water making beating doubly;
(2) the rotten slurry of the chicken of step (1) is heated to 60-80 ℃, insulation 10-20min transfers pH to 6.0-8.0, add the proteolytic enzyme that is equivalent to chicken weight 0.1%-1.0%,, be warming up to the enzyme that goes out about 100 ℃ of insulation 20min at 50-80 ℃ of following hydrolysis 2-4h, be cooled to below the room temperature, obtain enzymolysis solution;
(3) enzymolysis solution is centrifugal, collect contain black-bone chicken crude melanin part again drying promptly make black-bone chicken crude melanin.
Proteolytic enzyme of the present invention is selected papoid and/or trypsinase for use.
Described enzymolysis solution centrifugally operated condition is 3000-6000r/min, and the time is 20-40min.
The present invention adopt proteolytic enzyme under suitable temperature and neutrallty condition with the black-bone chicken enzymolysis, enzymolysis time shortens greatly, and enzymolysis solution centrifugation, drying are promptly made black-bone chicken crude melanin, purity is 50%-60%.
The applicant selects for use edible acid, edible alkali to regulate pH value through a large amount of test, for example selects edible acid such as phosphoric acid, citric acid for use, edible alkali for example: yellow soda ash, sodium bicarbonate etc., selected 50-80 ℃ is the enzymic hydrolysis temperature range, reaches the object of the invention.
Extracting resulting melanochrome molecular weight is: 217, and molecular formula is: C
13H
15O
2N, structure is roughly:
The present invention has following beneficial effect:
(1) simplify the melanin of black-bone chicken extracting method, simple;
(2) shorten extraction time greatly, cut down the consumption of energy, the extraction efficiency height;
(3), solved the anticorrosion problem of enzymolysis process effectively because of the shortening greatly of time;
(4) the black-bone chicken protein enzymatic hydrolyzate can also be used to prepare other products, and raw material is fully used.
(5) product that the present invention produced can be used for preparing health-related product, comprises capsule, tablet and pulvis etc., has increased health Taihe chicken foodstuff product new variety, can satisfy the ever-increasing demand of consumers in general, has very high economic benefit and social benefit.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in more detail, but do not limit the invention among the described embodiment.
Embodiment 1
(1) black-bone chicken takes by weighing 5kg light chicken through butchering unhairing, removing internal organ, rubs into meat gruel, adds the water making beating of 10kg.
(2) the rotten slurry of chicken is heated to 60 ℃, insulation 20min, adopt edible acid for example: phosphoric acid, citric acid etc. and edible alkali for example: yellow soda ash, sodium bicarbonate etc. are transferred pH to 6.0, add the 15g papoid, at 80 ℃ of following hydrolysis 2h, be warming up to the enzyme that goes out about 100 ℃ of insulation 20min, be cooled to below the room temperature enzymolysis solution that obtains.
(3) with enzymolysis solution centrifugal 20min under the 6000r/min condition, collect contain black-bone chicken crude melanin part again drying promptly make about 100g black-bone chicken crude melanin.
Embodiment 2
(1) black-bone chicken hair chicken takes by weighing 10kg light chicken through butchering unhairing, removing internal organ, rubs into meat gruel, adds the water making beating of 10kg.
(2) the rotten slurry of chicken is heated to 80 ℃, insulation 10min transfers pH to 8.0, adds 100kg trypsinase, at 50 ℃ of following hydrolysis 4h, is warming up to the enzyme that goes out about 100 ℃ of insulation 20min, is cooled to below the room temperature, obtains enzymolysis solution.
(3) with centrifugal 40min under the enzymolysis solution 3000r/min condition, collect contain black-bone chicken crude melanin part again drying promptly make about 200g black-bone chicken crude melanin.
Embodiment 3
(1) black-bone chicken hair chicken takes by weighing 10kg light chicken through butchering unhairing, removing internal organ, rubs into meat gruel, adds the water making beating of 15kg.
(2) the rotten slurry of chicken is heated to 70 ℃, insulation 15min transfers pH to 7.0, adds 50g papoid and 50g trypsinase, at 65 ℃ of following hydrolysis 3h, is warming up to the enzyme that goes out about 100 ℃ of insulation 20min, is cooled to below the room temperature enzymolysis solution that obtains.
(3) with centrifugal 30min under the enzymolysis solution 5000r/min condition, collect contain black-bone chicken crude melanin part again drying promptly make about 180g black-bone chicken crude melanin.
Claims (3)
1, a kind of extracting method of black-bone chicken crude melanin is characterized in that may further comprise the steps:
(1) black-bone chicken hair chicken is rubbed into meat gruel then through butchering unhairing, removing internal organ, adds 1-2 water making beating doubly;
(2) the rotten slurry of the chicken that step (1) is obtained is heated to 60-80 ℃, and insulation 10-20min transfers pH to 6.0-8.0, add the proteolytic enzyme that is equivalent to chicken weight 0.1%-1.0%, at 50-80 ℃ of following hydrolysis 2-4h, the enzyme that goes out heats up, be cooled to below the room temperature, obtain enzymolysis solution;
(3) with the enzymolysis solution centrifugation, collect contain black-bone chicken crude melanin part again drying promptly make black-bone chicken crude melanin.
2, extracting method according to claim 1 is characterized in that proteolytic enzyme is selected papoid and/or trypsinase for use described in the step (2).
3, extracting method according to claim 1 and 2 is characterized in that the centrifugation condition is 3000-6000r/min described in the step (3), and the time is 20-40min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710027969 CN101058821A (en) | 2007-05-11 | 2007-05-11 | Method for extracting black-bone chicken crude melanin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710027969 CN101058821A (en) | 2007-05-11 | 2007-05-11 | Method for extracting black-bone chicken crude melanin |
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| Publication Number | Publication Date |
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| CN101058821A true CN101058821A (en) | 2007-10-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710027969 Pending CN101058821A (en) | 2007-05-11 | 2007-05-11 | Method for extracting black-bone chicken crude melanin |
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| CN (1) | CN101058821A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106009767A (en) * | 2016-06-11 | 2016-10-12 | 江西倍得力生物工程有限公司 | Method for extracting melanin from Taihe black-bone silky fowl |
| CN106962571A (en) * | 2017-02-13 | 2017-07-21 | 南昌大学 | A kind of dark chocolate bar of the active component containing black-bone chicken and preparation method thereof |
| CN109054435A (en) * | 2018-09-10 | 2018-12-21 | 中国农业大学 | A method of extracting melanin from black-bone chicken periosteum |
| CN113354957A (en) * | 2021-07-08 | 2021-09-07 | 中国农业大学 | Method for efficiently extracting melanin from black-bone chicken tissues |
-
2007
- 2007-05-11 CN CN 200710027969 patent/CN101058821A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106009767A (en) * | 2016-06-11 | 2016-10-12 | 江西倍得力生物工程有限公司 | Method for extracting melanin from Taihe black-bone silky fowl |
| CN106962571A (en) * | 2017-02-13 | 2017-07-21 | 南昌大学 | A kind of dark chocolate bar of the active component containing black-bone chicken and preparation method thereof |
| CN109054435A (en) * | 2018-09-10 | 2018-12-21 | 中国农业大学 | A method of extracting melanin from black-bone chicken periosteum |
| CN109054435B (en) * | 2018-09-10 | 2021-03-02 | 中国农业大学 | Method for extracting melanin from periosteum of black-bone chicken |
| CN113354957A (en) * | 2021-07-08 | 2021-09-07 | 中国农业大学 | Method for efficiently extracting melanin from black-bone chicken tissues |
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Open date: 20071024 |