CN106009711A - Method for preparing fish gelatin - Google Patents
Method for preparing fish gelatin Download PDFInfo
- Publication number
- CN106009711A CN106009711A CN201610521301.8A CN201610521301A CN106009711A CN 106009711 A CN106009711 A CN 106009711A CN 201610521301 A CN201610521301 A CN 201610521301A CN 106009711 A CN106009711 A CN 106009711A
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- solution
- pectin
- isinglass
- mtgase
- fish gelatin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
- C08L89/04—Products derived from waste materials, e.g. horn, hoof or hair
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/005—Processes for mixing polymers
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
- C08J3/246—Intercrosslinking of at least two polymers
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
- C08J2389/04—Products derived from waste materials, e.g. horn, hoof or hair
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/06—Pectin; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
The invention discloses a method for preparing fish gelatin. The method includes the steps that scale gelatin, high-ester pectin and MTGase serve as raw materials, and a fish gelatin-pectin composite is prepared with an electrostatic method and a dynamic high-pressure micro-jetting technology, and then modified through the MTGase. According to the method, the fish gelatin-pectin composite is prepared with the electrostatic compounding method, a fish gelatin-pectin composite solution is micronized with the dynamic high-pressure micro-jetting technology, and the fish gelatin-pectin composite is formed through room-temperature hydration, and modified through the MTGase. The fish gelatin prepared with the method has high gel strength and gel melting temperature and good rheological properties, and can be widely applied to the fields of food and medicine. The method is easy to operate and high in practicability, a new method is provided for preparing the high-quality fish gelatin, and the range of the application of the fish gelatin to the fields of food and medicine is further expanded.
Description
Technical field
Can improve isinglass gel strength while of the present invention relates to a kind of, glue melts temperature and the high-quality isinglass preparation method of rheological properties, belongs to technical field of food preparation.
Background technology
Gelatin is the one of high-molecular gel, has preferable gelation and is widely used in the medicine food fields such as drug release, Yoghourt, medicated beer.At present, most gelatin that circulates in market is mammal gelatin, but the impact of fear, religions belief and the vegetarianism brought due to " bovine spongiform encephalopathy " and " foot and mouth disease ", the application of mammal gelatin receives certain restriction.Research finds, isinglass has the gelation similar with mammal gelatin, can be as the material of a kind of novel replacement mammal gelatin.But the gelation of isinglass, glue melt, and temperature is relatively low, rheological properties is poor.The most how to improve the functional characteristic of isinglass, producing high-quality isinglass is one of current study hotspot.
The functional characteristic method improving isinglass is a lot, such as electrostatic method, enzyme process, chemical method etc..But isinglass character prepared by electrostatic method is unstable, the phenomenon being separated easily occurs；Enzyme process does not ensures that the gel strength of isinglass and thermal stability are completely the same；Chemical method is readily incorporated new harmful substance.
Based on above-mentioned present situation, the present invention as raw material with grass carp scales gelatin, pectin, glutamine transaminage (MTGase), uses electrostatic composite algorithm, dynamic high-pressure micro jetting technology, enzyme cross-linking method to produce one and has high-gel strength and glue melts temperature and rheological properties preferable isinglass product.
Summary of the invention
It is an object of the invention to provide a kind of method preparing isinglass for prior art deficiency.
The present invention uses grass carp scales gelatin, high-esterpectin, and MTGase is raw material.First isinglass and high-esterpectin are formed isinglass-pectin composite solution by electrostatic method, processed its macro by dynamic high-pressure micro jetting technology, room temperature aquation forms isinglass-pectin complex, the MTGase using high concentration again modifies complex, and final production goes out a kind of high-gel strength and glue melts temperature and rheological properties is preferable and the high-quality isinglass product of thermal reversion.
Preparation method of the present invention is:
(1) preparation of isinglass, pectin solution: weigh fish scale gelatin, high-esterpectin, adds distilled water, and 50 DEG C of water-baths are dissolved, and is configured to fish scale gelatin solution that mass concentration is 4% respectively and mass concentration is the pectin solution of 1%;
(2) preparation of isinglass-pectin composite solution: fish scale gelatin solution step (1) prepared and pectin solution are respectively according to 1:1,2:1, the ratio of 1:2 mixes after being mixed into three kinds of solution again, and adjusts pH to 6.5 with the NaOH solution of 1M and the HCl solution of 1M;
(3) dynamic high-pressure microjet processes: isinglass-pectin composite solution step (2) prepared processes through dynamic high-pressure microjet, and pressure is 40-80Mpa, and number of processes is 2 times;Room temperature water 2h after process;
(4) MTGase modifies: isinglass step (3) obtained-pectin complex 40 DEG C heating in water bath, adds the MTGase enzyme that quality solubility is 0.06%, stirs, 40 DEG C of water-bath 20-60min, ice bath enzyme denaturing 15min；Wherein MTGase enzyme unit alive is 100u/g.
The feature of this product: the present invention is with fresh water fish scale gelatin, high-esterpectin, MTGase as raw material, application dynamic high-pressure micro jetting technology, produce a kind of high-gel strength and glue melts temperature and rheological properties is preferable and the high-quality isinglass product of thermal reversion, food, medicine and other fields can be widely used in.
Beneficial effects of the present invention: the present invention uses dynamic high-pressure micro jetting technology macro isinglass-pectin composite solution, forms uniform complex, contributes to the crosslinking of later stage enzyme process;Use MTGase to modify isinglass-pectin complex, form high-gel strength and glue and melt temperature and the strong high-quality isinglass product of rheology stability;This method is simple to operate, can apply to commercial production, and the isinglass product function character produced is good, thermal reversion, can be widely used in food, field of medicaments.
Detailed description of the invention
Embodiment one
1, the preparation of isinglass, pectin solution: weigh appropriate fish scale gelatin, high-esterpectin, is separately added into a certain amount of distilled water, and 50 DEG C of water-baths are dissolved, and being configured to concentration is 4%(w/v) fish scale gelatin solution and 1%(w/v) pectin solution.
2, the preparation of isinglass-pectin composite solution: the solution of above-mentioned preparation is pressed VFish scale gelatin: VPectinThe ratio mix homogeneously of=1:1, and adjust pH to 6.5 with the NaOH solution of 1M, HCl solution.
3, dynamic high-pressure microjet processes: being processed through dynamic high-pressure microjet by the isinglass of above-mentioned preparation-pectin composite solution, pressure is 40Mpa, and number of processes is 2 times.Room temperature water 2h after process.
4, MTGase modifies: by the isinglass after aquation-pectin complex 40 DEG C heating in water bath, adds 0.06%(w/v) MTGase enzyme, stir, 40 DEG C of water-bath 20min.Ice bath enzyme denaturing 15min；Wherein MTGase enzyme unit alive is 100u/g.
5, the present invention produces the high-quality isinglass obtained: isinglass gel strength prepared by the present invention is 470.12 ± 10.27g, and it is 29.25 ± 0.32 DEG C that glue melts temperature, and colloid solution is non-Newtonian fluid, and apparent viscosity maximum is 8.72 ± 0.37 Pa.s.
Embodiment two
1, the preparation of isinglass, pectin solution: weigh appropriate fish scale gelatin, high-esterpectin, is separately added into a certain amount of distilled water, and 50 DEG C of water-baths are dissolved, and being configured to concentration is 4%(w/v) fish scale gelatin solution and 1%(w/v) pectin solution.
2, the preparation of isinglass-pectin composite solution: the solution of above-mentioned preparation is pressed VFish scale gelatin: VPectinThe ratio mix homogeneously of=2:1, and adjust pH to 6.5 with the NaOH solution of 1M, HCl solution.
3, dynamic high-pressure microjet processes: being processed through dynamic high-pressure microjet by the isinglass of above-mentioned preparation-pectin composite solution, pressure is 60Mpa, and number of processes is 2 times.Room temperature water 2h after process.
4, MTGase modifies: by the isinglass after aquation-pectin complex 40 DEG C heating in water bath, adds 0.06%(w/v) MTGase enzyme, stir, 40 DEG C of water-bath 40min.Ice bath enzyme denaturing 15min；Wherein MTGase enzyme unit alive is 100u/g.
5, the present invention produces the high-quality isinglass obtained: isinglass gel strength prepared by the present invention is 527.36 ± 8.13g, and it is 31.23 ± 0.15 DEG C that glue melts temperature, and colloid solution is non-Newtonian fluid, and apparent viscosity maximum is 12.68 ± 0.14 Pa.s.
Embodiment three
1, the preparation of isinglass, pectin solution: weigh appropriate fish scale gelatin, high-esterpectin, is separately added into a certain amount of distilled water, and 50 DEG C of water-baths are dissolved, and being configured to concentration is 4%(w/v) fish scale gelatin solution and 1%(w/v) pectin solution.
2, the preparation of isinglass-pectin composite solution: the solution of above-mentioned preparation is pressed VFish scale gelatin: VPectinThe ratio mix homogeneously of=1:2, and adjust pH to 6.5 with the NaOH solution of 1M, HCl solution.
3, dynamic high-pressure microjet processes: being processed through dynamic high-pressure microjet by the isinglass of above-mentioned preparation-pectin composite solution, pressure is 80Mpa, and number of processes is 2 times.Room temperature water 2h after process.
4, MTGase modifies: by the isinglass after aquation-pectin complex 40 DEG C heating in water bath, adds 0.06%(w/v) MTGase enzyme, stir, 40 DEG C of water-bath 60min.Ice bath enzyme denaturing 15min；Wherein MTGase enzyme unit alive is 100u/g.
5, the present invention produces the high-quality isinglass obtained: isinglass gel strength prepared by the present invention is 503.69 ± 7.36g, and it is 30.49 ± 0.34 DEG C that glue melts temperature, and colloid solution is non-Newtonian fluid, and apparent viscosity maximum is 15.29 ± 0.27Pa.s.
Claims (1)
1. the method preparing isinglass, it is characterised in that: described method step is:
(1) preparation of isinglass, pectin solution: weigh fish scale gelatin, high-esterpectin, adds distilled water, and 50 DEG C of water-baths are dissolved, and is configured to fish scale gelatin solution that mass concentration is 4% respectively and mass concentration is the pectin solution of 1%;
(2) preparation of isinglass-pectin composite solution: fish scale gelatin solution step (1) prepared and pectin solution are respectively according to 1:1,2:1, the ratio of 1:2 mixes after being mixed into three kinds of solution again, and adjusts pH to 6.5 with the NaOH solution of 1M and the HCl solution of 1M;
(3) dynamic high-pressure microjet processes: isinglass-pectin composite solution step (2) prepared processes through dynamic high-pressure microjet, and pressure is 40-80Mpa, and number of processes is 2 times;Room temperature water 2h after process;
(4) MTGase modifies: isinglass step (3) obtained-pectin complex 40 DEG C heating in water bath, adds the MTGase enzyme that quality solubility is 0.06%, stirs, 40 DEG C of water-bath 20-60min, ice bath enzyme denaturing 15min；Wherein MTGase enzyme unit alive is 100u/g.
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| CN201610521301.8A CN106009711B (en) | 2016-07-05 | 2016-07-05 | A method of preparing isinglass |
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| CN201610521301.8A CN106009711B (en) | 2016-07-05 | 2016-07-05 | A method of preparing isinglass |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108219491A (en) * | 2018-02-26 | 2018-06-29 | 山东科达基建有限公司 | Colorful Asphalt Pavement |
| CN109275769A (en) * | 2018-10-18 | 2019-01-29 | 江西师范大学 | Method for making fish gelatin cotton candy |
| CN111264839A (en) * | 2020-03-25 | 2020-06-12 | 江西师范大学 | Modified fish gelatin, preparation method and application thereof, yogurt, low-fat set yogurt and preparation method thereof |
| CN111387474A (en) * | 2020-03-23 | 2020-07-10 | 江西师范大学 | High-quality fish gelatin and gilliding powder and preparation method thereof |
| CN113598351A (en) * | 2021-07-02 | 2021-11-05 | 江苏大学 | Preparation method of premna microphylla-fish scale compound gel |
| CN114052173A (en) * | 2021-11-23 | 2022-02-18 | 南京财经大学 | Novel method for delaying retrogradation of rice flour gel product |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1016347A1 (en) * | 1998-12-31 | 2000-07-05 | Skw Biosystems | Process for preparing fish gelatin |
| CN103059745A (en) * | 2012-12-15 | 2013-04-24 | 新昌县冠阳技术开发有限公司 | Preparation method of tilapia skin gelatin |
| CN104342040A (en) * | 2014-10-09 | 2015-02-11 | 江西师范大学 | Preparation method of high-melt-temperature fish scale gelatin |
| CN104448334A (en) * | 2014-11-20 | 2015-03-25 | 江西师范大学 | Method for increasing gelatin strength and gelatin melting temperature of fish gelatin |
-
2016
- 2016-07-05 CN CN201610521301.8A patent/CN106009711B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1016347A1 (en) * | 1998-12-31 | 2000-07-05 | Skw Biosystems | Process for preparing fish gelatin |
| CN103059745A (en) * | 2012-12-15 | 2013-04-24 | 新昌县冠阳技术开发有限公司 | Preparation method of tilapia skin gelatin |
| CN104342040A (en) * | 2014-10-09 | 2015-02-11 | 江西师范大学 | Preparation method of high-melt-temperature fish scale gelatin |
| CN104448334A (en) * | 2014-11-20 | 2015-03-25 | 江西师范大学 | Method for increasing gelatin strength and gelatin melting temperature of fish gelatin |
Non-Patent Citations (5)
| Title |
|---|
| 林亲录 等: "《食品工艺学》", 31 August 2014, 中南大学出版社 * |
| 汪多仁: "《绿色增塑剂》", 31 October 2011, 科学技术文献出版社 * |
| 涂宗财: "蛋白质动态超高压微射流改性研究及机理初探", 《中国博士学位论文全文数据库 工程科技I辑》 * |
| 迟玉杰: "《食品添加剂》", 30 April 2013, 中国轻工业出版社 * |
| 郑学晶 等: "《天然高分子材料》", 31 December 2010, 化学工业出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108219491A (en) * | 2018-02-26 | 2018-06-29 | 山东科达基建有限公司 | Colorful Asphalt Pavement |
| CN108219491B (en) * | 2018-02-26 | 2021-08-24 | 东营广通科技有限公司 | Color asphalt pavement |
| CN109275769A (en) * | 2018-10-18 | 2019-01-29 | 江西师范大学 | Method for making fish gelatin cotton candy |
| CN111387474A (en) * | 2020-03-23 | 2020-07-10 | 江西师范大学 | High-quality fish gelatin and gilliding powder and preparation method thereof |
| CN111264839A (en) * | 2020-03-25 | 2020-06-12 | 江西师范大学 | Modified fish gelatin, preparation method and application thereof, yogurt, low-fat set yogurt and preparation method thereof |
| CN111264839B (en) * | 2020-03-25 | 2023-06-09 | 江西师范大学 | Modified fish gelatin, preparation method and application thereof, and yoghurt and low-fat set yoghurt and preparation method thereof |
| CN113598351A (en) * | 2021-07-02 | 2021-11-05 | 江苏大学 | Preparation method of premna microphylla-fish scale compound gel |
| CN113598351B (en) * | 2021-07-02 | 2023-10-10 | 江苏大学 | Preparation method of Premna microphylla-fish scale compound gel |
| CN114052173A (en) * | 2021-11-23 | 2022-02-18 | 南京财经大学 | Novel method for delaying retrogradation of rice flour gel product |
| CN114052173B (en) * | 2021-11-23 | 2024-04-09 | 南京财经大学 | Novel method for delaying retrogradation of rice flour gel product |
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| CN106009711B (en) | 2018-07-17 |
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