CN106009711B - A method of preparing isinglass - Google Patents
A method of preparing isinglass Download PDFInfo
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- CN106009711B CN106009711B CN201610521301.8A CN201610521301A CN106009711B CN 106009711 B CN106009711 B CN 106009711B CN 201610521301 A CN201610521301 A CN 201610521301A CN 106009711 B CN106009711 B CN 106009711B
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- isinglass
- pectin
- solution
- mtgase
- water
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
- C08L89/04—Products derived from waste materials, e.g. horn, hoof or hair
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/005—Processes for mixing polymers
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
- C08J3/246—Intercrosslinking of at least two polymers
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
- C08J2389/04—Products derived from waste materials, e.g. horn, hoof or hair
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/06—Pectin; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
A method of isinglass being prepared, with fish scale gelatin, high-ester pectin, MTGase is raw material, prepares isinglass pectin complex using electrostatic method, dynamic high-pressure micro jetting technology, then modify isinglass pectin complex with MTGase.The present invention prepares isinglass pectin complex using electrostatic composite algorithm；Using dynamic high-pressure micro jetting technology macro isinglass pectin composite solution, room temperature aquation forms isinglass pectin complex；Isinglass pectin complex is modified using MTGase.There is isinglass prepared by this method higher gel strength and glue to melt temperature and good rheological properties, can be widely applied to field of food and medicine.This method is easy to operate, highly practical, and the not preparation of only high-quality isinglass provides a kind of new method, also further expands isinglass in food, the application of medicine and other fields.
Description
Technical field
The present invention relates to it is a kind of and meanwhile isinglass gel strength can be improved, glue melts temperature and the high-quality fish of rheological properties
Gelatin preparation method, belongs to technical field of food preparation.
Background technology
Gelatin is one kind of high-molecular gel, and there is preferable gelation to be widely used in drug release, Yoghourt, beer
In equal medicine foods field.Currently, most gelatin that circulates in the market is mammal gelatin, but due to " rabid ox disease " and " mouth
The application of the influence of fear, religious belief and vegetarianism that fever aphthous " is brought, mammal gelatin receives certain limitation.
The study found that isinglass has the gelation similar with mammal gelatin, a kind of novel replacement mammal gelatin can be used as
Material.But gelation, the glue of isinglass melt that temperature is relatively low, rheological properties are poor.Therefore the function of isinglass how is improved
Characteristic produces one of the research hotspot that high-quality isinglass is current.
There are many functional characteristic method for improving isinglass, such as electrostatic method, enzyme process, chemical method.But fish prepared by electrostatic method
Gelatin properties are unstable, the phenomenon that easily phase separation；Enzyme process does not ensure that the gel strength and thermal stability of isinglass
It is completely the same；Chemical method is readily incorporated new harmful substance.
Based on above-mentioned present situation, the present invention is with grass carp scales gelatin, pectin, glutamine transaminage（MTGase）For raw material,
Producing one kind using electrostatic composite algorithm, dynamic high-pressure micro jetting technology, enzyme crosslinking method, there is high-gel strength and glue to melt temperature
And the preferable isinglass product of rheological properties.
Invention content
The purpose of the present invention is provide a kind of method preparing isinglass for prior art deficiency.
The present invention uses grass carp scales gelatin, and high-ester pectin, MTGase is raw material.First isinglass and high-ester pectin are passed through
Electrostatic method forms isinglass-pectin composite solution, is handled its macro by dynamic high-pressure micro jetting technology, room temperature aquation is formed
Isinglass-pectin complex, then complex is modified using the MTGase of high concentration, final production goes out a kind of high-gel strength and glue
Melt temperature and rheological properties preferably and the high-quality isinglass product of thermal reversion.
Preparation method of the present invention is：
(1) preparation of isinglass, pectin solution：Fish scale gelatin, high-ester pectin are weighed, distilled water is added, 50 DEG C of water-baths are molten
Solution, is configured to the fish scale gelatin solution that mass concentration is 4% and the pectin solution that mass concentration is 1% respectively;
(2) preparation of isinglass-pectin composite solution：Fish scale gelatin solution and pectin solution point prepared by step (1)
Not according to 1:1, 2:1, 1:2 ratio is mixed again after being mixed into three kinds of solution, and the NaOH solution of 1M and the HCl of 1M is used in combination
Solution tune pH to 6.5;
(3) dynamic high-pressure microjet is handled：Isinglass-pectin composite solution prepared by step (2) is micro- through dynamic high-pressure
Jet stream processing, pressure 40-80Mpa, number of processes are 2 times;Room temperature water 2h after processing;
(4) MTGase is modified：It is molten that quality is added in the isinglass that step (3) is obtained -40 DEG C of pectin complex heating water bath
Degree is 0.06% MTGase enzymes, is stirred evenly, 40 DEG C of water-bath 20-60min, ice bath enzyme deactivation 15min；Wherein MTGase enzyme activity list
Position is 100u/g.
The feature of this product：The present invention is using fresh water fish scale gelatin, high-ester pectin, MTGase as raw material, using dynamic high-pressure
Micro jetting technology, produce a kind of high-gel strength and glue melt temperature and rheological properties preferably and the high-quality fish of thermal reversion
Gelatin product can be widely used in food, medicine and other fields.
Beneficial effects of the present invention:The present invention uses dynamic high-pressure micro jetting technology macro isinglass-pectin composite solution,
Uniform complex is formed, later stage enzyme process is contributed to be crosslinked;Isinglass-pectin complex is modified using MTGase, forms Gao Ning
Glue intensity and glue melt temperature and the strong high-quality isinglass product of rheology stability;This method is easy to operate, can be applied to
Industrial production, and the isinglass product function property produced is good, thermal reversion, can be widely used in field of food and medicine.
Specific implementation mode
Embodiment one
1, the preparation of isinglass, pectin solution：Suitable fish scale gelatin, high-ester pectin are weighed, is separately added into a certain amount of
Distilled water, 50 DEG C of water-bath dissolvings, is configured to a concentration of 4%（w/v）Fish scale gelatin solution and 1%（w/v）Pectin solution.
2, the preparation of isinglass-pectin composite solution：The solution of above-mentioned preparation is pressed into VFish scale gelatin：VPectin=1:1 ratio mixing
Uniformly, NaOH solution, the HCl solution tune pH to 6.5 of 1M is used in combination.
3, dynamic high-pressure microjet is handled：By the isinglass of above-mentioned preparation-pectin composite solution through dynamic high-pressure microjet
Processing, pressure 40Mpa, number of processes are 2 times.Room temperature water 2h after processing.
4, MTGase is modified：By the isinglass after aquation -40 DEG C of pectin complex heating water bath, it is added 0.06%（w/v）
MTGase enzymes, stir evenly, 40 DEG C of water-bath 20min.Ice bath enzyme deactivation 15min；Wherein MTGase enzyme-activity units are 100u/g.
5, the high-quality isinglass that present invention production obtains：Isinglass gel strength prepared by the present invention be 470.12 ±
10.27g, it is 29.25 ± 0.32 DEG C that glue, which melts temperature, and colloidal solution is non-newtonian fluid, apparent viscosity maximum value is 8.72 ±
0.37 Pa.s。
Embodiment two
1, the preparation of isinglass, pectin solution：Suitable fish scale gelatin, high-ester pectin are weighed, is separately added into a certain amount of
Distilled water, 50 DEG C of water-bath dissolvings, is configured to a concentration of 4%（w/v）Fish scale gelatin solution and 1%（w/v）Pectin solution.
2, the preparation of isinglass-pectin composite solution：The solution of above-mentioned preparation is pressed into VFish scale gelatin：VPectin=2:1 ratio mixing
Uniformly, NaOH solution, the HCl solution tune pH to 6.5 of 1M is used in combination.
3, dynamic high-pressure microjet is handled：By the isinglass of above-mentioned preparation-pectin composite solution through dynamic high-pressure microjet
Processing, pressure 60Mpa, number of processes are 2 times.Room temperature water 2h after processing.
4, MTGase is modified：By the isinglass after aquation -40 DEG C of pectin complex heating water bath, it is added 0.06%（w/v）
MTGase enzymes, stir evenly, 40 DEG C of water-bath 40min.Ice bath enzyme deactivation 15min；Wherein MTGase enzyme-activity units are 100u/g.
5, the high-quality isinglass that present invention production obtains：Isinglass gel strength prepared by the present invention be 527.36 ±
8.13g, it is 31.23 ± 0.15 DEG C that glue, which melts temperature, and colloidal solution is non-newtonian fluid, apparent viscosity maximum value is 12.68 ±
0.14 Pa.s。
Embodiment three
1, the preparation of isinglass, pectin solution：Suitable fish scale gelatin, high-ester pectin are weighed, is separately added into a certain amount of
Distilled water, 50 DEG C of water-bath dissolvings, is configured to a concentration of 4%（w/v）Fish scale gelatin solution and 1%（w/v）Pectin solution.
2, the preparation of isinglass-pectin composite solution：The solution of above-mentioned preparation is pressed into VFish scale gelatin：VPectin= 1:2 ratio is mixed
It closes uniformly, NaOH solution, the HCl solution tune pH to 6.5 of 1M is used in combination.
3, dynamic high-pressure microjet is handled：By the isinglass of above-mentioned preparation-pectin composite solution through dynamic high-pressure microjet
Processing, pressure 80Mpa, number of processes are 2 times.Room temperature water 2h after processing.
4, MTGase is modified：By the isinglass after aquation -40 DEG C of pectin complex heating water bath, it is added 0.06%（w/v）
MTGase enzymes, stir evenly, 40 DEG C of water-bath 60min.Ice bath enzyme deactivation 15min；Wherein MTGase enzyme-activity units are 100u/g.
5, the high-quality isinglass that present invention production obtains：Isinglass gel strength prepared by the present invention be 503.69 ±
7.36g, it is 30.49 ± 0.34 DEG C that glue, which melts temperature, and colloidal solution is non-newtonian fluid, apparent viscosity maximum value is 15.29 ±
0.27Pa.s。
Claims (1)
1. a kind of method preparing isinglass, it is characterised in that:The method step is:
(1) preparation of isinglass, pectin solution：Fish scale gelatin, high-ester pectin are weighed, distilled water is added, 50 DEG C of water-bath dissolvings divide
It is not configured to the fish scale gelatin solution that mass concentration is 4% and the pectin solution that mass concentration is 1%；
(2) preparation of isinglass-pectin composite solution：Fish scale gelatin solution and pectin solution prepared by step (1) are pressed respectively
According to 1:1,2:1,1:2 ratio is mixed again after being mixed into three kinds of solution, and the NaOH solution of 1M and the HCl solution tune of 1M is used in combination
PH to 6.5；
(3) dynamic high-pressure microjet is handled：Isinglass-pectin composite solution prepared by step (2) is through dynamic high-pressure microjet
Processing, pressure 40-80Mpa, number of processes are 2 times；Room temperature water 2h after processing；
(4) MTGase is modified：The isinglass that step (3) is obtained -40 DEG C of pectin complex heating water bath, quality solubility, which is added, is
0.06% MTGase enzymes, stir evenly, 40 DEG C of water-bath 20-60min, ice bath enzyme deactivation 15min；Wherein MTGase enzyme-activity units are
100u/g。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610521301.8A CN106009711B (en) | 2016-07-05 | 2016-07-05 | A method of preparing isinglass |
Applications Claiming Priority (1)
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| CN201610521301.8A CN106009711B (en) | 2016-07-05 | 2016-07-05 | A method of preparing isinglass |
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| Publication Number | Publication Date |
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| CN106009711A CN106009711A (en) | 2016-10-12 |
| CN106009711B true CN106009711B (en) | 2018-07-17 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108219491B (en) * | 2018-02-26 | 2021-08-24 | 东营广通科技有限公司 | Color asphalt pavement |
| CN109275769B (en) * | 2018-10-18 | 2021-10-22 | 江西师范大学 | Method for making fish gelatin cotton candy |
| CN111387474A (en) * | 2020-03-23 | 2020-07-10 | 江西师范大学 | High-quality fish gelatin and gilliding powder and preparation method thereof |
| CN111264839B (en) * | 2020-03-25 | 2023-06-09 | 江西师范大学 | Modified fish gelatin, preparation method and application thereof, and yoghurt and low-fat set yoghurt and preparation method thereof |
| CN113598351B (en) * | 2021-07-02 | 2023-10-10 | 江苏大学 | Preparation method of Premna microphylla-fish scale compound gel |
| CN114052173B (en) * | 2021-11-23 | 2024-04-09 | 南京财经大学 | Novel method for delaying retrogradation of rice flour gel product |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1016347A1 (en) * | 1998-12-31 | 2000-07-05 | Skw Biosystems | Process for preparing fish gelatin |
| CN103059745A (en) * | 2012-12-15 | 2013-04-24 | 新昌县冠阳技术开发有限公司 | Preparation method of tilapia skin gelatin |
| CN104342040A (en) * | 2014-10-09 | 2015-02-11 | 江西师范大学 | Preparation method of high-melt-temperature fish scale gelatin |
| CN104448334A (en) * | 2014-11-20 | 2015-03-25 | 江西师范大学 | Method for increasing gelatin strength and gelatin melting temperature of fish gelatin |
-
2016
- 2016-07-05 CN CN201610521301.8A patent/CN106009711B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1016347A1 (en) * | 1998-12-31 | 2000-07-05 | Skw Biosystems | Process for preparing fish gelatin |
| CN103059745A (en) * | 2012-12-15 | 2013-04-24 | 新昌县冠阳技术开发有限公司 | Preparation method of tilapia skin gelatin |
| CN104342040A (en) * | 2014-10-09 | 2015-02-11 | 江西师范大学 | Preparation method of high-melt-temperature fish scale gelatin |
| CN104448334A (en) * | 2014-11-20 | 2015-03-25 | 江西师范大学 | Method for increasing gelatin strength and gelatin melting temperature of fish gelatin |
Non-Patent Citations (1)
| Title |
|---|
| 蛋白质动态超高压微射流改性研究及机理初探;涂宗财;《中国博士学位论文全文数据库 工程科技I辑》;20071215(第06期);第211页第8.2节第2段 * |
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