CN104448334A - Method for increasing gelatin strength and gelatin melting temperature of fish gelatin - Google Patents
Method for increasing gelatin strength and gelatin melting temperature of fish gelatin Download PDFInfo
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- CN104448334A CN104448334A CN201410662374.XA CN201410662374A CN104448334A CN 104448334 A CN104448334 A CN 104448334A CN 201410662374 A CN201410662374 A CN 201410662374A CN 104448334 A CN104448334 A CN 104448334A
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Abstract
The invention discloses a method for increasing gelatin strength and a gelatin melting temperature of fish gelatin. The method comprises the following steps: by taking freshwater processing waste fish scales as raw materials, performing raw material pre-treatment, removing impurity proteins through a NaCl solution and a NaOH solution, performing n-butyl alcohol degreasing, performing water-bath extracting, performing microwave-oven drying, performing ultraviolet radiation, and the like, thereby preparing fish gelatin with high gelatin strength and a gelatin melting temperature. According to the method disclosed by the invention, the impurity proteins are removed through the NaCl solution and the NaOH solution, so that the prepared fish gelatin is relatively high in purity; microwave-oven drying is adopted, so that the drying time is shortened, and the original activity of the gelatin is greatly kept; ultraviolet radiation treatment is adopted to modify crucian fish scale gelatin. The method is pollution-free, simple, convenient and safe to operate, capable of increasing the gelatin strength and the gelatin melting temperature of the fish gelatin and broadening the range of application of the fish gelatin, and is a novel method for modifying the fish gelatin.
Description
Technical field
The present invention relates to and a kind ofly improve the method that isinglass Gel strength and glue melt temperature, the product of preparation can be widely used in medicine, field of food.
Background technology
Gelatin is by Mammals as the collagen hydro in the skin of pig, ox or bone forms, and can be widely used in the field such as medicine, food.But due to the impact of mad cow disease (BSE), foot and mouth disease (FDA) and toxic capsule, cause the security of Mammals gelatin to be subject to the query of people.Therefore, find a kind of novel gelatin materials and become one of focus of current research.
Research finds containing abundant collagen protein in fish scale, and isinglass and Mammals gelatin properties similar.But because isinglass contains low proline(Pro) and oxyproline, there is lower Gel strength and glue melts temperature, limit the range of application of isinglass.Both at home and abroad the report of relevant isinglass modification is a lot, modal be enzyme modified as glutamine transaminage (TG), but TG enzyme price is more expensive, and can cause gelatin heat irreversible when its consumption is too much, therefore has certain limitation.Uv-radiation refers to that wavelength is called uv-radiation in the electromagnetic radiation of 200-400nm, be widely used in food material, processing environment sterilization and improve the quality of food.
Based on above-mentioned present situation, the present invention intends by physical method, adopts ultraviolet radiation treatment isinglass, carries out modification to isinglass, thus improve its Gel strength, glue melts temperature.Modification for isinglass provides a kind of new method and thinking, thus widens its Application Areas.
Summary of the invention
The present invention aims to provide and a kind ofly improves the method that isinglass Gel strength and glue melt temperature.
With crucian fish scale for raw material, by tissue mashing, remove foreign protein, degreasing, extraction crucian fish scale gelatin, microwave-technology such as oven drying, uv irradiation, the Gel strength, the glue that improve isinglass melt temperature.
Its concrete technology step is as follows:
1, raw materials pretreatment: the impurity in rejecting fish scale is as iron wire, fish-bone etc., clean with tap water, and beat 0.5-2min by tissue mashing machine's intermittence, and rinse well with clear water;
2, foreign protein is removed:
(1) compound concentration is the NaCl solution of 5%, and add NaCl solution by after crucian fish scale tap water 3-5 time, the solid-liquid ratio g/mL of fish scale and NaCl solution is 1:1-1:3, soaks 30min, soaks 2 times;
(2) compound concentration is the NaOH solution of 0.4%, and the fish scale after soaking through NaCl solution is added NaOH solution, and the solid-liquid ratio g/mL of fish scale and NaOH solution is 1:1-1:3, soaks 1h, soaks 2 times;
3, degreasing: rinsed well by the fish scale clear water after removing foreign protein, adopts propyl carbinol degreasing 10-30min;
4, crucian fish scale gelatin extracts: by tap water 3-5 time of fish scale after degreasing, and regulate pH to make it identical with tap water pH, add distilled water, the solid-liquid ratio g/mL of fish scale and distilled water is 1:5, water-bath 60-80 DEG C, extraction 3h, and suction filtration, obtains supernatant liquor;
5, microwave-oven drying: first adopt microwave to carry out drying, power 400w, time of drying, 10-30min, drier in the baking oven being positioned over 40-60 DEG C;
6, uv irradiation process: pulverized by dried crucian fish scale gelatin pulverizer, in paving glass disc after sterilization, thickness 3mm, employing power is the ultraviolet lamp photograph of 40w, and the time is 0.5-2h.
The feature of this product: the present invention with crucian fish scale waste for raw material, extracted by foreign protein removal, crucian fish scale gelatin, the technology such as microwave-oven drying, uv irradiation process, chemistry, physical method is used to extract gelatin and adopt physical method to carry out modification to it, the Gel strength and the glue that improve crucian fish scale gelatin melt temperature, modification for fish scale gelatin provides a kind of new method, decreases the pollution of fish scale to environment, solves the problem of fish scale waste material waste.
Beneficial effect of the present invention: take freshwater fish scale as raw material, extracts gelatin, improves its waste ' s reclamation and is worth, decrease environmental pollution; Adopt salt, alkali removal of impurities, increase the purity of collagen protein; Adopt microwave-oven drying mode to carry out drying, not only can reduce the activity that time of drying also maintains collagen greatly; The modification mode of employing uv irradiation improves Gel strength, glue melts temperature, and technique is pollution-free, modification isinglass that is simple to operate, preparation is reversible, and the modification for isinglass provides new approaches.
Specific embodiments
Embodiment 1
1, raw materials pretreatment: the impurity in rejecting fish scale is as iron wire, fish-bone etc., clean with tap water, and beat 0.5min by tissue mashing machine's intermittence.And rinse well with clear water.
2, remove foreign protein: (1) compound concentration is the NaCl solution of 5%, add NaCl solution by after crucian fish scale tap water 3-5 time, the solid-liquid ratio g/mL of fish scale and NaCl solution is 1:1, and immersion 30min, soaks 2 times;
(2) compound concentration is the NaOH solution of 0.4%, and the fish scale after soaking through NaCl solution is added NaOH solution, and the solid-liquid ratio g/mL of fish scale and NaOH solution is 1:1, soaks 1h, soaks 2 times;
3, degreasing: rinsed well by the fish scale clear water after removing foreign protein, adopts propyl carbinol degreasing 10min;
4, crucian fish scale gelatin extracts: by tap water 3-5 time of fish scale after degreasing, and regulate pH to make it identical with tap water pH, add distilled water, the solid-liquid ratio g/mL of fish scale and distilled water is 1:5, water-bath 60 DEG C, extraction 3h, and suction filtration, obtains supernatant liquor;
5, microwave-oven drying: first adopt microwave to carry out drying, power 400w, time of drying, 10min, drier in the baking oven being positioned over 40 DEG C.
6, uv irradiation process: pulverized by dried crucian fish scale gelatin pulverizer, in paving glass disc after sterilization, thickness 3mm, employing power is the ultraviolet lamp photograph of 40w, and the time is 0.5h.
Embodiment 2
1, raw materials pretreatment: the impurity in rejecting fish scale is as iron wire, fish-bone etc., clean with tap water, and beat 1min by tissue mashing machine's intermittence.And rinse well with clear water.
2, remove foreign protein: (1) compound concentration is the NaCl solution of 5%, add NaCl solution by after crucian fish scale tap water 3-5 time, the solid-liquid ratio g/mL of fish scale and NaCl solution is 1:2, and immersion 30min, soaks 2 times;
(2) compound concentration is the NaOH solution of 0.4%, and the fish scale after soaking through NaCl solution is added NaOH solution, and the solid-liquid ratio g/mL of fish scale and NaOH solution is 1:2, soaks 1h, soaks 2 times;
3, degreasing: rinsed well by the fish scale clear water after removing foreign protein, adopts propyl carbinol degreasing 20min;
4, crucian fish scale gelatin extracts: by tap water 3-5 time of fish scale after degreasing, and regulate pH to make it identical with tap water pH, add distilled water, the solid-liquid ratio g/mL of fish scale and distilled water is 1:5, water-bath 70 DEG C, extraction 3h, and suction filtration, obtains supernatant liquor;
5, microwave-oven drying: first adopt microwave to carry out drying, power 400w, time of drying, 15min, drier in the baking oven being positioned over 50 DEG C.
6, uv irradiation reason: pulverized by dried crucian fish scale gelatin pulverizer, in paving glass disc after sterilization, thickness 3mm, employing power is the ultraviolet lamp photograph of 40w, and the time is 1h.
Embodiment 3
1, raw materials pretreatment: the impurity in rejecting fish scale is as iron wire, fish-bone etc., clean with tap water, and beat 1.5min by tissue mashing machine's intermittence.And rinse well with clear water.
2, remove foreign protein: (1) compound concentration is the NaCl solution of 5%, add NaCl solution by after crucian fish scale tap water 3-5 time, the solid-liquid ratio g/mL of fish scale and NaCl solution is 1:3, and immersion 30min, soaks 2 times;
(2) compound concentration is the NaOH solution of 0.4%, and the fish scale after soaking through NaCl solution is added NaOH solution, and the solid-liquid ratio g/mL of fish scale and NaOH solution is 1:3, soaks 1h, soaks 2 times;
3, degreasing: rinsed well by the fish scale clear water after removing foreign protein, adopts propyl carbinol degreasing 30min;
4, crucian fish scale gelatin extracts: by tap water 3-5 time of fish scale after degreasing, and regulate pH to make it identical with tap water pH, add distilled water, the solid-liquid ratio g/mL of fish scale and distilled water is 1:5, water-bath 80 DEG C, extraction 3h, and suction filtration, obtains supernatant liquor;
5, microwave-oven drying: first adopt microwave to carry out drying, power 400w, time of drying, 20min, drier in the baking oven being positioned over 60 DEG C.
6, uv irradiation process: pulverized by dried crucian fish scale gelatin pulverizer, in paving glass disc after sterilization, thickness 3mm, employing power is the ultraviolet lamp photograph of 40w, and the time is 1.5h.
Claims (4)
1. improve the method that isinglass Gel strength and glue melt temperature, it is characterized in that:
(1) raw materials pretreatment: reject iron wire, the fish-bone in fish scale, clean with tap water, and beat 0.5-2min by tissue mashing machine's intermittence, and rinse well with clear water;
(2) foreign protein is removed:
(A) prepare NaCl solution, add NaCl solution by after crucian fish scale tap water 3-5 time, immersion 30min, soaks 2 times;
(B) prepare NaOH solution, the fish scale after soaking through NaCl solution is added NaOH solution, soak 1h, soak 2 times;
(3) degreasing: rinsed well by the fish scale clear water after removing foreign protein, adopts propyl carbinol degreasing 10-30min;
(4) crucian fish scale gelatin extracts: by tap water 3-5 time of fish scale after degreasing, and regulate pH to make it identical with tap water pH, add distilled water, the solid-liquid ratio g/mL of fish scale and distilled water is 1:5, water-bath 60-80 DEG C, extraction 3h, and suction filtration, obtains supernatant liquor;
(5) supernatant liquor that step (4) obtains is carried out microwave-oven drying;
(6) uv irradiation process: pulverized by dried crucian fish scale gelatin pulverizer, in paving glass disc after sterilization, ultraviolet lamp is according to process.
2. a kind of according to claims 1 improves the method that isinglass Gel strength and glue melt temperature, and it is characterized in that: the NaCl solution concentration in above-mentioned steps (2) is 5%, the solid-liquid ratio g/mL of fish scale and NaCl solution is 1:1-1:3; NaOH solution concentration is 0.4%, and the solid-liquid ratio g/mL of fish scale and NaOH solution is 1:1-1:3.
3. a kind of according to claims 1 improves the method that isinglass Gel strength and glue melt temperature, it is characterized in that: in above-mentioned steps (5), the condition of microwave drying is: power 400w, time of drying 10-30min, drying temperature 40-60 DEG C.
4. a kind of according to claims 1 improves the method that isinglass Gel strength and glue melt temperature, it is characterized in that: the thickness being layered on crucian fish scale gelatin in glass disc described in above-mentioned steps (6) is 3mm, ultraviolet lamp power is 40w, and light application time is 0.5-2h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106009711A (en) * | 2016-07-05 | 2016-10-12 | 南昌大学 | Method for preparing fish gelatin |
| CN109337948A (en) * | 2018-10-18 | 2019-02-15 | 江西师范大学 | A method of it improving isinglass glue and melts temperature and gel strength |
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| US6271350B1 (en) * | 1998-08-11 | 2001-08-07 | Thara & Company Ltd. | Fish collagen and method of producing same |
| CN102051052A (en) * | 2010-11-17 | 2011-05-11 | 河南工业大学 | Method for preparing modifying agent for improving gel performance of fish scale gelatin |
| CN102524395A (en) * | 2011-12-23 | 2012-07-04 | 南昌大学 | Method for preparing solidified type yoghourt by applying fish scale gelatin |
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| US6271350B1 (en) * | 1998-08-11 | 2001-08-07 | Thara & Company Ltd. | Fish collagen and method of producing same |
| CN102051052A (en) * | 2010-11-17 | 2011-05-11 | 河南工业大学 | Method for preparing modifying agent for improving gel performance of fish scale gelatin |
| CN102524395A (en) * | 2011-12-23 | 2012-07-04 | 南昌大学 | Method for preparing solidified type yoghourt by applying fish scale gelatin |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106009711A (en) * | 2016-07-05 | 2016-10-12 | 南昌大学 | Method for preparing fish gelatin |
| CN106009711B (en) * | 2016-07-05 | 2018-07-17 | 南昌大学 | A method of preparing isinglass |
| CN109337948A (en) * | 2018-10-18 | 2019-02-15 | 江西师范大学 | A method of it improving isinglass glue and melts temperature and gel strength |
| CN109337948B (en) * | 2018-10-18 | 2021-10-01 | 江西师范大学 | Method for improving melting temperature and gel strength of fish gelatin |
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