CN1060016C

CN1060016C - Biocide and yield increasing agent and method for preparing same

Info

Publication number: CN1060016C
Application number: CN95113714A
Authority: CN
Inventors: 张凤春
Original assignee: LUBO BIOLOGICAL ENGINEERING-PRODUCTS FACTORY TIANSHUI CITY
Current assignee: LUBO BIOLOGICAL ENGINEERING-PRODUCTS FACTORY TIANSHUI CITY
Priority date: 1995-07-27
Filing date: 1995-07-27
Publication date: 2001-01-03
Anticipated expiration: 2015-07-27
Also published as: CN1141120A

Abstract

The present invention discloses an agent for killing bacteria and increasing yield, and relates to a preparation method of the agent, wherein the agent for killing bacteria and increasing yield is extracted by using chaetopoda oligochaeta invertebrate as raw material. The agent for killing bacteria and increasing yield comprises various amino acids, nucleotides, fulvic acids and various trace elements which are needed by plants. The present invention is a pure biological agent which solves the problem that inorganic fertilizers are largely used, and then, the soil is acidified and hardened; the present invention also solves the problem of the damage of the toxicity of pesticide residues to people and domestic animals. The present invention has unique treating efficiency for insect pests of plants, and especially for diseases caused by various fungi; the present invention combines the functions of insect disinfestation, insect banishment, sterilization and nutrition into integration, and the yield and the income of the crops can be increased under the condition of reducing the consumption of the fertilizer.

Description

A kind of bactericidal yield-increase agent and preparation method thereof

The invention belongs to plant growth regulator, particularly contain the plant growth regulator of microorganism or animal material manufacturing or extraction.

As everyone knows, soil chronic administration inorganic chemical fertilizer just can harden in acidifying, not only influences the growth of plant, and easily makes plant produce various damage by disease and insect.The residual hazard of agricultural chemicals is apparent to the harm of people and animals and crop.Find in the investigation, big many of the harm that more than insect pest crop is caused by various fungus-caused plant diseases, and also these diseases are infectious more.In recent years, some plant growth regulator of releasing, though in addressing the above problem, played certain effect, also quite obvious to the effect of increasing income of agricultural, for example " guarantor overcharges ", " triacontanol " etc. can improve the crop photosynthesis effect, promote seed grouting, " basic element of cell division " can divide by irritation cell, promotes growth and development of plants and chlorophyllous formation, improves disease resistance, the cold resistance of plant; " like farming " etc. can promote that crop seed roots.But its effect is single, can not improve the immunologic function of plant itself comprehensively, and the damage by disease and insect in prevention and the treatment growing process is especially by various fungus-caused diseases.Use respectively, time-consuming, the cost height, and mostly be inorganic compound, more or less demonstrate the defective of inorganic fertilizer and agricultural chemicals.

Purpose of the present invention just is to provide a kind of multiple damage by disease and insect that produces in the growing process that can prevent and treat, improve the immunologic function of plant itself comprehensively, supply with plant needed various nutrients in process of growth, promote growth and development of plants effectively, strengthen the biochemical function of crop, improve plant drought, cold-resistant and wound back healing ability, and to without any side effects to people and animals behind the plant-spraying.Soil is not had the pure biologic product of any pollution, when reducing the inorganic fertilizer consumption, the output value output of crops is improved.

The agent of this bactericidal yield-increase is the pure biologic product that the animal propagated artificially of a kind of utilization is extracted for primary raw material, it contains 18 seed amino acids such as L-aminobutanedioic acid, threonine, serine, glutamic acid, glycine, isoleucine, phenyl alanine, thick propylhomoserin, proline, and total amount is 58.73mg/100ml; Nucleotide 3.0ug/ml; Lipase active 2600 international units, full N62mg/l, full P0.046mg/l, full K4194mg/l; Al, B, Ba, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, Zn, 15 kinds of trace elements of S, total amount is 124.3PPM.

The preparation method of this bactericidal yield-increase agent is divided into the two large divisions:

1. breed part

1. the no vertebra chaetopoda Oligotrichida animal that screening breeding growth is fast is for culturing object;

2. with the bacterium bag of bovine, cultivated mushroom each half, screening is mixed into feed after pulverizing, and spray contains the aqueous solution of micro-total amounts 1% such as Cu, Fe, B, Zn, Ba, Na, Ni, S, K, Mg, Mn, Mo, makes feed humidity reach the above-mentioned animal of nursing 80% or more;

3. stir the animal in the feed on schedule, and with ovum, excrement, adult separately, will culture temperature and be controlled at 20 ℃-30 ℃, feed humidity remains on more than 80%.

2. production part

1. selecting vegetative period is that raw material is inserted in the rinse bath and cleaned greater than trimestral adult;

2. the raw material after will cleaning is inserted in the cracker and is ground, and slurries are sealed;

3. rapidly the animal slurries are poured in the dipping tank, added 50 times water, add hydrochloric acid again and transfer the solution pH value to stir extraction to 1.0-2.0, the extraction time was greater than 20 hours;

4. extract is carried out Separation of Solid and Liquid;

5. in parting liquid, add auxiliary material, as humus etc.;

6. the ratio adding emulsifier in 100: 0.5 disperses and emulsification treatment in above-mentioned mixed liquor, and emulsification times was treated post precipitation with Solid-Liquid Separation greater than 4 hours, and the pH value that adds ammoniacal liquor adjustment solution promptly obtains the agent of this bactericidal yield-increase to 8.0-9.0;

7. chemically examine, detect and enter hopper after qualified and carry out the canned production process of promptly finishing.

The agent of this bactericidal yield-increase can clearly be seen by above-mentioned production process, it is a kind of pure biologic product, since the trace element that adds in feed through extracting again after the annelids digestion thereby not having any what toxic and side effect, use very safe, crops are behind seed soaking or foliage-spray, can enter in the cyton of crop rapidly, enhance metabolism, strengthen photosynthesis, promote growth and development of plants, to the treatment fruit tree putrefaction disease, dry rot, booth vegetable downy mildew, the field corn mosaic disease, the fungal disease aspect effect of various crop such as anthracnose of grape is very remarkable.

Through in ground experiments such as Tianshui Bei Dao district flower ox, Bai Ya, big willow, Zhao Ya, obtain positive effect, as shown in the table: bactericidal yield-increase agent field experiment table

Experimental period days	Experimental period	The crop title	User's name	Experiment area (mu)	Dosing ml	What damage by disease and insect is arranged	Plant performance before the medication	Result of use
Experimental period days	Experimental period	The crop title	User's name	Experiment area (mu)	Dosing ml	What damage by disease and insect is arranged	Plant performance before the medication	Result of use	95．6	One week	Corn	Liu Chengfu	0．4	?200	The aphid mosaic disease	The inharmonious yellow leaf of growing is withered	Aphid all thirst, and the floral leaf scab moves back entirely, leaf dark green
95．6	All around	The cucumber grape	Wu Tianquan	0．2 0．5	?100 ?200	The downy mildew anthrachose of grape	The withered and yellow fruit of the ill variegated leaf sheet of blade is sparse	Scab takes off, and growth is accelerated	95．6	One week	Corn	Liu Chengfu	0．4	?200	The aphid mosaic disease	The inharmonious yellow leaf of growing is withered
95．6	All around							Scab takes off, and growth is accelerated	95．6	45 days	It is green that blade changes, state of an illness recovery from illness, and the fruit grain increases and coloring phenomenon is arranged
95．7	20 days							Apple	95．6	45 days		Lv Jinxi	6．0	?500	Two spotted spider mite	The serious leaf rolling of insect pest	Insect pest is controlled after three days, and fruit growth is accelerated, and the painted time in advance

In the experiment, do not using under the situation of any chemical fertilizer, spray the agent of this bactericidal yield-increase, not only multiple damage by disease and insect is is effectively prevented and treated, and plant growing is accelerated, and blade diameter is obviously than big before spraying, and plant strain is high obviously to be increased, fruit expands, and fruit such as grape, apple painted had remarkable effect.

Have on the contracted field of depositing Zhao Ya village Liu, wheat seed with the agent of this bactericidal yield-increase, is planted after soaking seed, discovery was emerged than control group (not soaking seed) Zao two days, and it is neat to emerge, and emergence rate reaches more than 95%, after one week, with comparing of not soaking seed, wheat seeding is obviously sturdy, and fibrous root is many, blade is long and thick, the leaf look dark green, do not see damage by disease and insect, and growing way obviously is better than the wheat without seed soaking.

Spray the agent of this bactericidal yield-increase at the tomato young fruit period, plant height increases than control group behind the first quarter moon, estimates according to planting the tomato vegetable grower for many years, do not using under the chemical fertilizer situation, amount of increase in production will reach about 35%, and tomato is had dematuration, can go on the market ahead of time, increase economic benefit.

Smear experiment according to another, the pears of dry rot rotten, apple tree to suffering from, the performance that shows, two pear trees of big willow village Wu Xue loyalty suffer from dry rot, the state of an illness is identical, to wherein one bark struck off a circle, smear the agent of this bactericidal yield-increase, and cover with wet soil, after 20 days, be coated with loblolly tree recovery from illness, and give birth to branch and come into leaves, scrape skin portion and begin to grow again bark.Another does not have the contrast tree of coating, and is withered dead, forms sharp contrast with the coating tree, be coated with loblolly tree and do not have the damage by disease and insect generation over the past half year again, grow fine, suffer from serious rot with the precious apple tree of village Nie Buddhist, trunk is most of to rot, lose growing ability, blade is withered to curl, and branch is drained, and is at death's door, smear the agent of this bactericidal yield-increase in the affected part, after one week, the no longer expansion of sick place is after secondary is used first quarter moon, the state of an illness is controlled, blade begins to unfold, and turns green, and branch development is good, disease portion begins healing, all healings after 20 days.With stalwartness tree growth no significant difference, after January, the leaf look dark green, the shoot ramp, and fruit expands significantly.

Above-mentioned experimental result shows that this bactericidal yield-increase agent scope of application in agricultural production is extensive, and the effect of increasing income is obvious, can reduce chemical fertilizer and pesticide dosage significantly, reduces agriculture production cost.

Below be one embodiment of the present of invention:

1. breed part

(1) with the earthworm is cultivated animals

(2) with doing cow dung 1000kg, bacterium bag 1000kg after the cultivated mushroom, the aqueous solution 300kg that sprays micro-total amounts 1% such as Cu, B, Ba, Ca, Cr, Fe, K, Mg, Mn, Na, Ni, P, Zn, S makes feed, its humidity is reached feed earthworm 1000kg more than 80%.The earthworm that stirred in per 7 days in the feed separates ovum, excrement, adult, will culture temperature and be controlled in the 20-30 ℃ of scope, and humidity remains on more than 80%.

2. production part

(1) with vegetative period greater than trimestral adult about 3 tons as raw material, insert rinse bath and clean;

(2) after the raw material after will cleaning grinds with cracker, slurries are inserted storage liquid irritate sealing;

(3) the animal slurries are poured in the dipping tank, behind the water that adding animal slurries are 50 times, added hydrochloric acid again and transfer the solution pH value to 1.0-2.0 stirring extraction, 24 hours extraction time;

(4) with screen pack extract is carried out Separation of Solid and Liquid;

(5) in parting liquid, by the sodium humate solution of 50: 1 volume ratios addings 60%;

(6) add in 100: 0.50 ratio that emulsifier disperses and emulsification treatment 5 hours, treat post precipitation with Solid-Liquid Separation, the pH value of adjusting solution with ammoniacal liquor promptly obtains this bactericidal yield-increase agent to 8.0-9.0;

(7) through chemical examination, detect and enter receiver after qualified and carry out cannedly, finish preparation process.

Claims

1. bactericidal yield-increase agent, it is characterized in that: the bactericidal yield-increase agent is to be raw material to propagate earthworm artificially, through cleaning, grind, pulverize, flood extract and separate, adding sodium humate, emulsifier are handled 18 seed amino acids that contain that obtain, total amount 58.73mg/100ml, nucleotide 3ug/ml, lipase active 2600 international units, fulvic acid 200ppm, contain full nitrogen 62mg/l, contain Cu, Fe, Al, B, Ba, Ca, Cr, K, Mg, Na, Ni, P, S, Zn, 15 kinds of trace elements of Mn, the biologic product of total amount 124.3PPm.

2. the preparation method of the described bactericidal yield-increase agent of claim 1 may further comprise the steps:

1. be the forage feed earthworm with the bacterium bag after cow dung and the cultivated mushroom;

2. extraction is cleaned, grinds, pulverizes, flooded to the one-tenth earthworm that will culture more than three months;

3. the extract Separation of Solid and Liquid is got its clear liquid;

4. add sodium humate in clear liquid, emulsifier carries out emulsification treatment and treats more than 20 hours that post precipitation carries out Separation of Solid and Liquid again;

5. in parting liquid, add alkaline solution and adjust pH value, make the bactericidal yield-increase agent to 8.0-9.0.

3. the preparation method of bactericidal yield-increase agent according to claim 2, it is characterized in that wherein feeding earthworm is to spray feed with 1% aqueous solution that contains Al, B, Ba, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, S, 15 kinds of mineral elements of Zn, feed humidity is remained on more than 80%, after animal digestion absorbs, extract.