CN105998453A - Traditional Chinese medicine for treating gout and hyperuricemia - Google Patents
Traditional Chinese medicine for treating gout and hyperuricemia Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/59—Menispermaceae (Moonseed family), e.g. hyperbaena or coralbead
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/884—Alismataceae (Water-plantain family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention relates to a traditional Chinese medicine, in particular to a traditional Chinese medicine for treating gout and hyperuricemia, and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine is prepared from, by weight, 10-100 parts of cortex phellodendri, 14-88 parts of radix sophorae flavescentis, 9-60 parts of radix stephaniae tetrandrae, 7-86 parts of rhizoma alismatis, 11.5-70 parts of rhizoma polygoni cuspidate and 10-130 parts of radix angelica sinensis. The active components of the traditional Chinese medicine are combined based on the traditional Chinese medicine differentiation theory, and synergistic interaction is realized through compatibility of the active components. In-vivo pharmacodynamic tests prove that the traditional Chinese medicine can remarkably reduce the level of uric acid and urea nitrogen, and has an obvious treatment effect on gout and hyperuricemia.
Description
Technical field
The present invention relates to a kind of Chinese medicine, particularly relate to the Chinese medicine for treating gout and hyperuricemia, it belongs to
In technical field of Chinese medicines.
Background technology
Hyperuricemia (HUA) refers under normal purine diet state, non-twice fasting blood uric acid level male on the same day
Higher than 420 μm ol/L, women is higher than 360 μm ol/L, the most referred to as hyperuricemia.
Along with development of modern society economy, the change of people's dietary structure, in food, purine content increases, and HUA presents height
Popular, rejuvenation, male are higher than women, the coastal trend higher than interior ground.HUA is multiple cardiovascular risk factors and relevant disease
Disease includes metabolism syndrome, 2 patients with type Ⅰ DM, hypertension, cardiovascular event and the independent hazard factor of death, nephropathy etc..Separately
Outward, HUA is an important stage of gout pathological development, and gout is considered as " the most dead cancer ", and harm can not look down upon.Closely
Nian Lai, have developed the preferable medicine of a lot of curative effect for HUA with gout both at home and abroad, but these medicines is respectively provided with certain poison
Side effect.Therefore, seek to prevent and treat hyperuricemia and gout is efficient, side effect is little medicine is the focus of current research.
The pharmaceutical pack of the most clinical common treatment hyperuricemia and gout is containing suppressing the medicine of uric acid synthesis and increasing urine
The medicine of acid excretion, it represents medicine and is respectively allopurinol and benzbromarone.Allopurinol and metabolite oxypurinol thereof
Make uricopoiesis reduce by the activity of suppression xanthine oxidase, thus reach therapeutic purposes.Benzbromarone can suppress urine
Hydrochlorate, at the active reabsorption of renal tubules, increases uratic excretion, thus reduces uratic concentration in blood.Can alleviate or anti-
Only the generation of urate crystal, subtracts pauciarticular damage, also can promote the dissolving of established urate crystal.Although above-mentioned two
The curative effect of kind of medicine is preferable, and clinical practice is extensive, but its untoward reaction include gastrointestinal symptom, erythra, liver function injury,
Bone marrow depression etc. drastically influence the quality of life of patient.
Chinese medicine, as side effect less draft class medicine, is widely applied to treat various disease.2014 by
Hyperuricemia that medical association endocrinology branch of China delivers and the Chinese Consensus of experts of gout treatment just propose Chinese medicine control
Treat gout and HUA is increasingly subject to people and pays close attention to.As the ethnopharmacology resource of China, some of which have antiinflammatory, ease pain, live
The effect of blood, detumescence and diuresis, this matches with the basic pathogenesis of hyperuricemia.This research is with antiinflammatory, detumescence, pain relieving, profit
Urine carries out formula for main principle, and modern pharmacological research shows, it can significantly reduce serum uric acid level, maintains uric acid level
Stable within normal range, there is the effect of preferable inhibiting hyperuricemia and gout.
Summary of the invention
It is an object of the invention to provide Chinese medicine for treating gout and hyperuricemia and preparation method thereof, the present invention
Chinese medicine has on inhibiting hyperuricemia and gout exposure basis, and it can significantly reduce uric acid and urea nitrogen content, the secondary work of poison
By less, good effect.
In order to achieve the above object, the present invention is achieved through the following technical solutions:
For treating the Chinese medicine of gout and hyperuricemia, it is made up by described parts by weight of following crude drug: Cortex Phellodendri 10
-100 parts, Radix Sophorae Flavescentis 14-88 parts, Radix Stephaniae Tetrandrae 9-60 parts, Rhizoma Alismatis 7-86 parts, Rhizoma Polygoni Cuspidati 11.5-70 parts, Radix Angelicae Sinensis 10-
130 parts.
The above-mentioned Chinese medicine for treating gout and hyperuricemia, it is by described weight portion numeral system by following crude drug
Become: Cortex Phellodendri 40-60 parts, Radix Sophorae Flavescentis 45-60 parts, Radix Stephaniae Tetrandrae 30-40 parts, Rhizoma Alismatis 35-45 parts, Rhizoma Polygoni Cuspidati 35-45 parts, when
Return 50-70 parts.
The preparation method of the above-mentioned Chinese medicine for treating gout and hyperuricemia, its step is as follows:
(1) take and meet the Cortex Phellodendri of described parts by weight, with the 75 % alcohol reflux 2 times of 6-8 times of medical material amounts, each 1-
2 hours, united extraction liquid, to filter, filtrate is concentrated to give primary extract；By primary extract styrene type low pole or nonpolar macroporous tree
Fat adsorbs, and purifies after washing post with 6-8 times of column volume 50 % ethanol solution eluting with 1-3 times of column volume, collection eluent
Recycling design, concentrate drying, obtain Cortex Phellodendri extract, standby；
(2) take and meet the Radix Sophorae Flavescentis of described parts by weight, with 60-80 % alcohol reflux 3 times of 6-10 times of medical material amounts, often
Secondary 1 hour, united extraction liquid, to filter, filtrate is concentrated to give primary extract, primary extract styrene type non-polar macroporous resin is inhaled
Attached, purify with 1-3 times of column volume and reclaim with 3-5 times of column volume 80 % ethanol solution eluting, collection eluent after washing post
Solvent, concentrate drying, obtain Radix Sophorae Flavescentis extract, standby.
(3) take and meet the Radix Stephaniae Tetrandrae of described parts by weight, with the 70 % alcohol reflux 1-2 time of 7-9 times of medical material amounts,
Each 2-3 h, filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate, concentrate
Obtain just extract；By primary extract by styrene type low pole macroporous resin adsorption and with 8 times of column volume 90 % ethanol solution eluting,
Collect eluent recycling design, be drying to obtain Radix Stephaniae Tetrandrae extract, standby；
(4) take and meet the Rhizoma Polygoni Cuspidati of described parts by weight, with 70-80 % alcohol reflux 1-2 of 8-10 times of medical material amounts
Secondary, each 1.5 h, filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate, dense
Contract, be drying to obtain Rhizoma Polygoni Cuspidati extract, standby；
(5) take and meet the Radix Angelicae Sinensis of described parts by weight, with 60-80 % alcohol reflux 1 time of 8-10 times of medical material amounts, often
Secondary 2 hours, united extraction liquid, to filter, filtrate is concentrated to give primary extract, is inhaled by primary extract styrene type low pole macroporous resin
Attached, purify with 1-3 times of column volume and reclaim with 8-10 times of column volume 60 % ethanol solution eluting, collection eluent after washing post
Solvent, concentrate drying, obtain Radix Angelicae Sinensis extract, standby；
(6) take and meet the Rhizoma Alismatis of described parts by weight, with 8-10 times amount 80-90 % alcohol reflux 2 times, each 1 h,
United extraction liquid, filters, and filtrate is concentrated to give primary extract, primary extract styrene type non-polar macroporous resin is adsorbed, with 1-3
With 6-8 times of column volume 60 % ethanol solution eluting after times column volume purification washing post, collect eluent recycling design, concentrate and do
Dry, obtain Rhizoma Alismatis extract, standby；
(7) Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract and Rhizoma Alismatis extract are taken,
It is equipped with corresponding adjuvant and makes capsule, tablet, granule, pill peroral dosage form.
For treating the Chinese medicine of gout and hyperuricemia, it is to be mixed with by described parts by weight by following crude drug
Form: Cortex Phellodendri extract 1-9 part, Radix Sophorae Flavescentis extract 2-12 parts, Radix Stephaniae Tetrandrae extract 2-12 parts, Rhizoma Polygoni Cuspidati extract 3-
18 parts, Radix Angelicae Sinensis extract 2-24 parts and Rhizoma Alismatis extract 2-24 parts.
The preparation method of above-mentioned Cortex Phellodendri extract: by Cortex Phellodendri 75 % alcohol reflux 2 of 6-8 times of medical material amounts
Secondary, each 1-2 hour, united extraction liquid, to filter, filtrate is concentrated to give primary extract；By primary extract with styrene type low pole or
Non-polar macroporous resin is adsorbed, and purifies with 1-3 times of column volume and washes with 6-8 times of column volume 50 % ethanol solution after washing post
De-, collect eluent recycling design, concentrate drying, obtain Cortex Phellodendri extract.
The preparation method of above-mentioned Radix Sophorae Flavescentis extract: by Radix Sophorae Flavescentis 60-80 % alcohol refluxs of 6-10 times of medical material amounts
Extract 3 times, each 1 hour, united extraction liquid, filter, filtrate is concentrated to give primary extract, by nonpolar for primary extract styrene type greatly
Hole resin absorption, purifies after washing post with 3-5 times of column volume 80 % ethanol solution eluting with 1-3 times of column volume, and collection is washed
De-liquid recycling design, concentrate drying, obtain Radix Sophorae Flavescentis extract.
The preparation method of above-mentioned Radix Stephaniae Tetrandrae extract: by Radix Stephaniae Tetrandrae 70 % alcohol reflux 1 of 7-9 times of medical material amounts-
2 times, each 2-3 h, filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate,
It is concentrated to give just extract；By primary extract by styrene type low pole macroporous resin adsorption and with 8 times of column volume 90 % ethanol solution
Eluting, collects eluent recycling design, is drying to obtain Radix Stephaniae Tetrandrae extract.
The preparation method of above-mentioned Rhizoma Polygoni Cuspidati extract: 70-80 % ethanol that Rhizoma Polygoni Cuspidati is drunk 8-10 times of medical material amounts return
Stream extraction 1-2 time, each 1.5 h, filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and
Above-mentioned filtrate, concentrates, is drying to obtain Rhizoma Polygoni Cuspidati extract.
The preparation method of above-mentioned Radix Angelicae Sinensis extract: by Radix Angelicae Sinensis 60-80 % alcohol refluxs of 8-10 times of medical material amounts
Extracting 1 time, each 2 hours, united extraction liquid, filtration, filtrate was concentrated to give primary extract, by big for primary extract styrene type low pole
Hole resin absorption, purifies after washing post with 8-10 times of column volume 60 % ethanol solution eluting with 1-3 times of column volume, and collection is washed
De-liquid recycling design, concentrate drying, obtain Radix Angelicae Sinensis extract；The preparation method of described Rhizoma Alismatis extract: Rhizoma Alismatis decoction pieces is washed
Only, dry, pulverize and sieve, by 8-10 times amount 80-90 % alcohol reflux 2 times, each 1 h, united extraction liquid, mistake
Filter, filtrate is concentrated to give primary extract, primary extract styrene type non-polar macroporous resin is adsorbed, purifies with 1-3 times of column volume
With 6-8 times of column volume 60 % ethanol solution eluting after washing post, collect eluent recycling design, concentrate drying, obtain Rhizoma Alismatis
Extract.
The preparation method of above-mentioned Cortex Phellodendri extract: by Cortex Phellodendri 75% alcohol reflux 2 times of 6-8 times of medical material amount, often
Secondary 1-2 hour, united extraction liquid, filter, filtrate is concentrated to give primary extract, by primary extract styrene type low pole or nonpolar greatly
Hole resin absorption, purifies with 1-3 times of column volume and returns with 6-8 times of column volume 50% ethanol solution eluting, collection eluent after washing post
Receive solvent, concentrate drying, obtain Cortex Phellodendri extract；The preparation method of Radix Sophorae Flavescentis extract: by the Radix Sophorae Flavescentis 60-of 6-10 times of medical material amount
80% alcohol reflux 3 times, each 1 hour, united extraction liquid, to filter, filtrate is concentrated to give primary extract, and primary extract is used benzene second
Ene-type non-polar macroporous resin is adsorbed, and purifies after washing post with 3-5 times of column volume 80% ethanol solution eluting with 1-3 times of column volume,
Collect eluent recycling design, concentrate drying, obtain Radix Sophorae Flavescentis extract；The preparation method of Radix Stephaniae Tetrandrae extract: by Radix Stephaniae Tetrandrae with 7-9 times
70% alcohol reflux of medical material amount 1-2 time, each 2-3h, filters, obtains filtrate and filtering residue；By filtering residue use water reflux, extract, 2
Secondary, united extraction liquid and above-mentioned filtrate, it is concentrated to give just extract, by primary extract by styrene type low pole macroporous resin adsorption also
With 8 times of column volume 90% ethanol solution eluting, collect eluent recycling design, be drying to obtain Radix Stephaniae Tetrandrae extract；Rhizoma Polygoni Cuspidati extract
Preparation method: by Rhizoma Polygoni Cuspidati 70-80% alcohol reflux 1-2 time of 8-10 times of medical material amount, each 1.5h, filter, obtain filtrate and
Filtering residue, by filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate, concentrates, is drying to obtain Rhizoma Polygoni Cuspidati extract；Radix Angelicae Sinensis
The preparation method of extract: by the Radix Angelicae Sinensis 60-80% alcohol reflux 1 time of 8-10 times of medical material amount, each 2 hours, merging carried
Taking liquid, filter, filtrate is concentrated to give primary extract, by primary extract styrene type low pole macroporous resin adsorption, with 1-3 times of column volume
With 8-10 times of column volume 60% ethanol solution eluting after purification washing post, collection eluent recycling design, concentrate drying, the most proper
Return extract；The preparation method of Rhizoma Alismatis extract: by Rhizoma Alismatis 8-10 times amount 80-90% alcohol reflux 2 times, each 1h, close
And extracting solution, to filter, filtrate is concentrated to give primary extract, primary extract styrene type non-polar macroporous resin is adsorbed, with 1-3 times of post
With 6-8 times of column volume 60% ethanol solution eluting after volume purification washing post, collect eluent recycling design, concentrate drying, to obtain final product
Rhizoma Alismatis extract.
Feature and the effect of the present invention are as follows:
The present invention is directed to gout pathogenic factor and sickness rate present situation thereof, by Cortex Phellodendri, Radix Sophorae Flavescentis, Radix Stephaniae Tetrandrae, Rhizoma Alismatis, Rhizoma Polygoni Cuspidati, Radix Angelicae Sinensis raw material
Medicine is made, and each active component forms according to Chinese medical discrimination theory, and the compatibility of each active component has reached the effect of Synergistic.This
Invention can be prepared to various conventional dosage form for oral administration, verifies through the internal test of pesticide effectiveness, and Chinese medicine of the present invention can significantly reduce urine
Acid and urea nitrogen levels, evident in efficacy to hyperuricemia and gout, can be used for treating gout new Chinese medicine, there is wide city
Field application prospect.
Wherein:
Cortex Phellodendri is rutaceae wampeePhellodendron chinenseSchneid. dry bark.Bitter in the mouth, property
Cold；Return kidney, bladder warp；There is heat clearing and damp drying, pathogenic fire purging except steaming, effect of detoxification sore treatment；Can be used for damp-heat dysentery, jaundice dark coloured urine,
Leukorrhagia pudendal pruritus, the puckery pain of pyretic stranguria, tinea pedis flaccidity is preesed, hectic fever due to YIN-deficiency consumptive fever, night sweat, seminal emission, sore swollen toxin, eczema eczema.Modern pharmacology grinds
Study carefully the curative effect showing that Cortex Phellodendri extract has gout and inhibiting hyperuricemia, it is possible to significantly reduce uric acid level, may be with it
There is the pharmacological action such as antioxidation, protection kidney, antiinflammatory, Culture in vitro relevant.
Radix Sophorae Flavescentis is leguminous plant Radix Sophorae FlavescentisSophora flavescensAit. dry root.Bitter in the mouth, cold in nature；GUIXIN, liver,
Stomach, large intestine, bladder warp；There is heat clearing and damp drying, parasite killing, effect of diuresis；Can be used for hematodiarrhoea, have blood in stool, jaundice urine retention, red and white leukorrhea
Under, swelling of the vulva pudendal pruritus, eczema, eczema, skin pruritus, scabies leprosy, external treatment trichomonal vaginitis.Radix Sophorae Flavescentis and the diuresis of extract thereof
Effect may produce positive role to the treatment of hyperuricemia.
Radix Stephaniae Tetrandrae is menispermaceous plants Radix stephaniae tetrandraeStephania tetrandraThe dry root of S.Moore.Acrid in the mouth, hardship, property
Cold；Return bladder, lung meridian；There is wind-expelling pain-stopping, effect of inducing diuresis to remove edema；Can be used for rheumatic arthralgia, edema tinea pedis, dysuria,
Eczema sore.Its effect and hyperuricemia and the disease of gout and coincide, be applied in the middle of the compound recipe of multiple gout.
Rhizoma Alismatis is Notes On Alism At Aceae Rhizoma AlismatisAlisma orientalis(Sam.)Juzep.Dry tuber.It is sweet in the mouth, light,
Cold in nature；Return kidney, bladder warp；There is promoting diuresis to eliminate damp pathogen, the effect of the turbid blood fat reducing that expels the heat-evil, changes；Can be used for dysuria, edema distension, let out
Rush down oliguria, dizziness due to fluid-retention, the puckery pain of pyretic stranguria, hyperlipemia.
Rhizoma Polygoni Cuspidati is polygonaceae plant Rhizoma Polygoni CuspidatiPolygonum cuspidatumThe dry rhizome of Sieb.et Zucc. and root.Taste
Micro-hardship, cold nature；Return liver, gallbladder, lung meridian.There is effect of dampness removing jaundice eliminating, heat-clearing and toxic substances removing, eliminating stasis to stop pain, relieving cough and resolving phlegm；Can be used for
Jaundice due to damp-heat, stranguria with turbid discharge, leukorrhagia, rheumatic arthralgia, carbuncle sore tumefacting virus, burn due to hot liquid or fire, amenorrhea, lump in the abdomen, instep beat damage, cough due to lung-heat.
When the root being classified as samphire Angelica sinensis (Oliv.) Diels.Sweet in the mouth, pungent, warm in nature；Return
Liver, the heart, spleen channel；There is effect of replenishing and activating blood, menstruction regulating and pain relieving, loosening bowel to relieve constipation.For blood deficiency and yellow complexion, dizziness cardiopalmus, menstruation not
Tune, amenorrhea dysmenorrhea, sweating due to debility stomachache, rheumatic arthralgia, injury from falling down, ulcer sores, dryness of the intestine constipation etc..
Instant component prescriptions of Chinese medicine can add various customary adjuvant required when preparing different dosage form, is prepared as any
A kind of conventional peroral dosage form, such as powder, pill, capsule, granule, tablet etc..
The present invention is according to theory of Chinese medical science, each active component is prepared from according to certain proportion compatibility, through internal drug effect
Learning verification experimental verification, it can significantly reduce hyperuricemia (gout) Mouse Blood and urine uric acid and the level of blood urea nitrogen, alleviates
Clinical disease, toxic and side effects is little, evident in efficacy, achieves satisfied curative effect in gout treatment.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further.Following example are only the several concrete reality of the present invention
Execute example, but the design concept of the present invention is not limited thereto, all changes utilizing this design that the present invention carries out unsubstantiality, all
The behavior invading scope should be belonged to.
Method in following embodiment, if no special instructions, is conventional method.
The following percentage composition in embodiment if no special instructions, is weight/mass percentage composition.
Embodiment 1
For treating the Chinese medicine of gout and hyperuricemia, it is made up by described parts by weight of following crude drug: Cortex Phellodendri 10
Part, Radix Sophorae Flavescentis 14 parts, Radix Stephaniae Tetrandrae 60 parts, Rhizoma Alismatis 86 parts, Rhizoma Polygoni Cuspidati 70 parts, Radix Angelicae Sinensis 130 parts.
Its preparation method is as follows:
(1) Cortex Phellodendri taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, carry with 8 times of medical material amount 75 % alcohol refluxs
Taking 2 times, each 1 hour, collect extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, by weak for first extract styrene type
Polarity or non-polar macroporous resin absorption, with after 2 times of column volumes washing posts with 6 times of column volume 50 % ethanol solution eluting, collection
Eluent recycling design, concentrate drying, obtain Cortex Phellodendri extract；
(2) Radix Sophorae Flavescentis taking described parts by weight cleans, dry, pulverize after cross 60 mesh sieves, carry with 8 times of medical material amount 75 % alcohol refluxs
Take 3 times, each 1 hour, and and extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, will first extract, first extract is used
Styrene type low pole or non-polar macroporous resin absorption, wash after posts with 5 times of column volume 80 % ethanol solution with 3 times of column volumes
Eluting, collects eluent recycling design, is dried, obtains Radix Sophorae Flavescentis extract.
(3) Radix Stephaniae Tetrandrae taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, with the ethanol of 8 times of medical material amounts 70%
Reflux, extract, 2 times, each 2 hours, the filtrate of filtration and filtering residue, reflux, extract, that filtering residue is added water 2 times, collect extracting solution, merge
Filtrate and extracting solution, be concentrated to give just extract, first extract styrene type low pole or non-polar macroporous resin is adsorbed
(medicinal liquid resin than for 1:1), with 8 times of column volume 90 ethanol solution eluting after 3 times of column volumes washing posts, collects eluent recovery
Solvent, is dried, obtains Radix Stephaniae Tetrandrae extract.
(4) Rhizoma Polygoni Cuspidati taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, with 10 times of medical material amount 70 % ethanol
Reflux, extract, 2 times, each 1.5 h, filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned
Filtrate, concentrates, is drying to obtain Rhizoma Polygoni Cuspidati extract.
(5) Radix Angelicae Sinensis taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, with 10 times of medical material amount 80 % ethanol
Reflux, extract, 1 time, extracts 2 hours, united extraction liquid, filters, and filtrate is concentrated to give primary extract, by weak for primary extract styrene type pole
Property macroporous resin adsorption, purify after washing post with 8 times of column volume 60 % ethanol solution eluting with 2 times of column volumes, collection eluent
Recycling design, concentrate drying, obtain Radix Angelicae Sinensis extract.
(6) Rhizoma Alismatis taking described parts by weight cleans, dry, pulverize after cross 20 mesh sieves, carry with 8 times amount 80 % alcohol refluxs
Taking 2 times, each 1 h, united extraction liquid, filter, filtrate is concentrated to give primary extract, by nonpolar macroporous for primary extract styrene type tree
Fat adsorbs, and purifies after washing post with 6 times of column volume 60 % ethanol solution eluting with 2 times of column volumes, collection eluent recycling design,
Concentrate drying, obtains Rhizoma Alismatis extract.
(7) take above-mentioned prepare Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract
Thing and Rhizoma Alismatis extract, be equipped with corresponding additive of tablet, make tablet.
Embodiment 2
For treating the Chinese medicine of gout and hyperuricemia, it is made up by described parts by weight of following crude drug: Cortex Phellodendri 100
Part, Radix Sophorae Flavescentis 88 parts, Radix Stephaniae Tetrandrae 7 parts, Rhizoma Alismatis 11.5 parts, Rhizoma Polygoni Cuspidati 10 parts, Radix Angelicae Sinensis 130 parts.
Its preparation method is as follows:
(1) Cortex Phellodendri taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, carry with 6 times of medical material amount 75 % alcohol refluxs
Taking 2 times, each 2 hours, collect extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, by weak for first extract styrene type
Polarity or non-polar macroporous resin absorption, with after 1 times of column volume washing post with 8 times of column volume 50 % ethanol solution eluting, collection
Eluent recycling design, concentrate drying, obtain Cortex Phellodendri extract；
(2) Radix Sophorae Flavescentis taking described parts by weight cleans, dry, pulverize after cross 60 mesh sieves, carry with 6 times of medical material amount 60 % alcohol refluxs
Take 3 times, each 1 hour, and and extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, will first extract, first extract is used
Styrene type low pole or non-polar macroporous resin absorption, wash after post with 3 times of column volume 80 % ethanol solution with 1 times of column volume
Eluting, collects eluent recycling design, is dried, obtains Radix Sophorae Flavescentis extract.
(3) Radix Stephaniae Tetrandrae taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, with the ethanol of 7 times of medical material amount 70 %
Reflux, extract, 1 time, each 3 hours, the filtrate of filtration and filtering residue, reflux, extract, that filtering residue is added water 2 times, collect extracting solution, merge
Filtrate and extracting solution, be concentrated to give just extract, first extract styrene type low pole or non-polar macroporous resin is adsorbed
(medicinal liquid resin than for 1:1), with 8 times of column volume 90 % ethanol solution eluting after 3 times of column volumes washing posts, collects eluent recovery
Solvent, is dried, obtains Radix Stephaniae Tetrandrae extract.
(4) Rhizoma Polygoni Cuspidati taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 8 times of medical material amount 80 % ethanol
Stream extracts 1 time, each 1.5 h, filter, obtains filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filter
Liquid, concentrates, is drying to obtain Rhizoma Polygoni Cuspidati extract.
(5) Radix Angelicae Sinensis taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 8 times of medical material amount 60 % ethanol
Stream extracts 1 time, extracts 2 hours, united extraction liquid, filters, and filtrate is concentrated to give primary extract, and primary extract is used styrene type low pole
Macroporous resin adsorption, purifies with 1 times of column volume and returns with 10 times of column volume 60 % ethanol solution eluting, collection eluent after washing post
Receive solvent, concentrate drying, obtain Radix Angelicae Sinensis extract.
(6) Rhizoma Alismatis taking described parts by weight cleans, dry, pulverize after cross 20 mesh sieves, with 10 times amount 90 % alcohol refluxs
Extracting 2 times, each 1 h, united extraction liquid, filter, filtrate is concentrated to give primary extract, by nonpolar macroporous for primary extract styrene type
Resin absorption, purifies after washing post with 8 times of column volume 60 % ethanol solution eluting with 1 times of column volume, collects eluent recovery molten
Agent, concentrate drying, obtain Rhizoma Alismatis extract.
(7) take above-mentioned prepare Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract
Thing and Rhizoma Alismatis extract, be equipped with corresponding additive of tablet, make capsule.
Embodiment 3
For treating the Chinese medicine of gout and hyperuricemia, it is made up by described parts by weight of following crude drug: Cortex Phellodendri 40
Part, Radix Sophorae Flavescentis 45 parts, Radix Stephaniae Tetrandrae 40 parts, Rhizoma Alismatis 45 parts, Rhizoma Polygoni Cuspidati 45 parts, Radix Angelicae Sinensis 70 parts.
Its preparation method is as follows:
(1) Cortex Phellodendri taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, carry with 7 times of medical material amount 75 % alcohol refluxs
Taking 2 times, each 2 hours, collect extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, by weak for first extract styrene type
Polarity or non-polar macroporous resin absorption, with after 3 times of column volumes washing posts with 7 times of column volume 50 % ethanol solution eluting, collection
Eluent recycling design, concentrate drying, obtain Cortex Phellodendri extract；
(2) Radix Sophorae Flavescentis taking described parts by weight cleans, dry, pulverize after cross 60 mesh sieves, with 10 times of medical material amount 80 % alcohol refluxs
Extract 3 times, each 1 hour, and and extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, will first extract, by first extract
Adsorb by styrene type low pole or non-polar macroporous resin, with molten with 4 times of column volume 80 % ethanol after 2 times of column volume washing posts
Liquid eluting, collects eluent recycling design, is dried, obtains Radix Sophorae Flavescentis extract.
(3) Radix Stephaniae Tetrandrae taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, with the ethanol of 9 times of medical material amount 70 %
Reflux, extract, 1 time, each 2.5 hours, the filtrate of filtration and filtering residue, reflux, extract, that filtering residue is added water 2 times, collect extracting solution, close
And filtrate and extracting solution, it is concentrated to give just extract, by first extract styrene type low pole or non-polar macroporous resin suction
Attached (medicinal liquid resin than for 1:1), returns with 8 times of column volume 90 % ethanol solution eluting, collection eluent after 3 times of column volumes washing posts
Receive solvent, be dried, obtain Radix Stephaniae Tetrandrae extract.
(4) Rhizoma Polygoni Cuspidati taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 9 times of medical material amount 75% ethanol
Stream extracts 1 time, each 1.5 h, filter, obtains filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filter
Liquid, concentrates, is drying to obtain Rhizoma Polygoni Cuspidati extract.
(5) Radix Angelicae Sinensis taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 9 times of medical material amount 70 % ethanol
Stream extracts 1 time, extracts 2 hours, united extraction liquid, filters, and filtrate is concentrated to give primary extract, and primary extract is used styrene type low pole
Macroporous resin adsorption, purifies with 3 times of column volumes and returns with 9 times of column volume 60 % ethanol solution eluting, collection eluent after washing post
Receive solvent, concentrate drying, obtain Radix Angelicae Sinensis extract.
(6) Rhizoma Alismatis taking described parts by weight cleans, dry, pulverize after cross 20 mesh sieves, carry with 9 times amount 85 % alcohol refluxs
Taking 2 times, each 1 h, united extraction liquid, filter, filtrate is concentrated to give primary extract, by nonpolar macroporous for primary extract styrene type tree
Fat adsorbs, and purifies after washing post with 7 times of column volume 60 % ethanol solution eluting with 3 times of column volumes, collection eluent recycling design,
Concentrate drying, obtains Rhizoma Alismatis extract.
(7) take above-mentioned prepare Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract
Thing and Rhizoma Alismatis extract, be equipped with corresponding additive of tablet, make granule.
Embodiment 4
For treating the Chinese medicine of gout and hyperuricemia, it is made up by described parts by weight of following crude drug: Cortex Phellodendri 40
Part, Radix Sophorae Flavescentis 45 parts, Radix Stephaniae Tetrandrae 40 parts, Rhizoma Alismatis 45 parts, Rhizoma Polygoni Cuspidati 45 parts, Radix Angelicae Sinensis 70 parts.
Its preparation method is as follows:
(1) Cortex Phellodendri taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, carry with 7 times of medical material amount 75 % alcohol refluxs
Taking 2 times, each 2 hours, collect extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, by weak for first extract styrene type
Polarity or non-polar macroporous resin absorption, with after 3 times of column volumes washing posts with 7 times of column volume 50 % ethanol solution eluting, collection
Eluent recycling design, concentrate drying, obtain Cortex Phellodendri extract；
(2) Radix Sophorae Flavescentis taking described parts by weight cleans, dry, pulverize after cross 60 mesh sieves, with 10 times of medical material amount 80 % alcohol refluxs
Extract 3 times, each 1 hour, and and extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, will first extract, by first extract
Adsorb by styrene type low pole or non-polar macroporous resin, with molten with 4 times of column volume 80 % ethanol after 2 times of column volume washing posts
Liquid eluting, collects eluent recycling design, is dried, obtains Radix Sophorae Flavescentis extract.
(3) Radix Stephaniae Tetrandrae taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, with the ethanol of 9 times of medical material amount 70 %
Reflux, extract, 1 time, each 2.5 hours, the filtrate of filtration and filtering residue, reflux, extract, that filtering residue is added water 2 times, collect extracting solution, close
And filtrate and extracting solution, it is concentrated to give just extract, by first extract styrene type low pole or non-polar macroporous resin suction
Attached (medicinal liquid resin than for 1:1), returns with 8 times of column volume 90 % ethanol solution eluting, collection eluent after 3 times of column volumes washing posts
Receive solvent, be dried, obtain Radix Stephaniae Tetrandrae extract.
(4) Rhizoma Polygoni Cuspidati taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 9 times of medical material amount 75 % ethanol
Stream extracts 1 time, each 1.5 h, filter, obtains filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filter
Liquid, concentrates, is drying to obtain Rhizoma Polygoni Cuspidati extract.
(5) Radix Angelicae Sinensis taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 9 times of medical material amount 70 % ethanol
Stream extracts 1 time, extracts 2 hours, united extraction liquid, filters, and filtrate is concentrated to give primary extract, and primary extract is used styrene type low pole
Macroporous resin adsorption, purifies with 3 times of column volumes and returns with 9 times of column volume 60 % ethanol solution eluting, collection eluent after washing post
Receive solvent, concentrate drying, obtain Radix Angelicae Sinensis extract.
(6) Rhizoma Alismatis taking described parts by weight cleans, dry, pulverize after cross 20 mesh sieves, carry with 9 times amount 85 % alcohol refluxs
Taking 2 times, each 1 h, united extraction liquid, filter, filtrate is concentrated to give primary extract, by nonpolar macroporous for primary extract styrene type tree
Fat adsorbs, and purifies after washing post with 7 times of column volume 60 % ethanol solution eluting with 3 times of column volumes, collection eluent recycling design,
Concentrate drying, obtains Rhizoma Alismatis extract.
(7) take above-mentioned prepare Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract
Thing and Rhizoma Alismatis extract, be equipped with corresponding additive of tablet, make powder.
Embodiment 5
For treating the Chinese medicine of gout and hyperuricemia, it is made up by described parts by weight of following crude drug: Cortex Phellodendri 60
Part, Radix Sophorae Flavescentis 60 parts, Radix Stephaniae Tetrandrae 30, Rhizoma Alismatis 35 parts, Rhizoma Polygoni Cuspidati 35 parts, Radix Angelicae Sinensis 50 parts.
Its preparation method is as follows:
(1) Cortex Phellodendri taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, carry with 7 times of medical material amount 75 % alcohol refluxs
Taking 2 times, each 2 hours, collect extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, by weak for first extract styrene type
Polarity or non-polar macroporous resin absorption, with after 3 times of column volumes washing posts with 7 times of column volume 50 % ethanol solution eluting, collection
Eluent recycling design, concentrate drying, obtain Cortex Phellodendri extract；
(2) Radix Sophorae Flavescentis taking described parts by weight cleans, dry, pulverize after cross 60 mesh sieves, with 10 times of medical material amount 80 % alcohol refluxs
Extract 3 times, each 1 hour, and and extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, will first extract, by first extract
Adsorb by styrene type low pole or non-polar macroporous resin, with molten with 4 times of column volume 80 % ethanol after 2 times of column volume washing posts
Liquid eluting, collects eluent recycling design, is dried, obtains Radix Sophorae Flavescentis extract.
(3) Radix Stephaniae Tetrandrae taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, with the ethanol of 8 times of medical material amount 70 %
Reflux, extract, 1 time, each 2.5 hours, the filtrate of filtration and filtering residue, reflux, extract, that filtering residue is added water 2 times, collect extracting solution, close
And filtrate and extracting solution, it is concentrated to give just extract, by first extract styrene type low pole or non-polar macroporous resin suction
Attached (medicinal liquid resin than for 1:1), returns with 8 times of column volume 90 % ethanol solution eluting, collection eluent after 3 times of column volumes washing posts
Receive solvent, be dried, obtain Radix Stephaniae Tetrandrae extract.
(4) Rhizoma Polygoni Cuspidati taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 9 times of medical material amount 75% ethanol
Stream extracts 2 times, each 1.5h, filter, obtains filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filter
Liquid, concentrates, is drying to obtain Rhizoma Polygoni Cuspidati extract.
(5) Radix Angelicae Sinensis taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 8 times of medical material amount 70 % ethanol
Stream extracts 1 time, extracts 2 hours, united extraction liquid, filters, and filtrate is concentrated to give primary extract, and primary extract is used styrene type low pole
Macroporous resin adsorption, purifies with 2 times of column volumes and returns with 9 times of column volume 60 % ethanol solution eluting, collection eluent after washing post
Receive solvent, concentrate drying, obtain Radix Angelicae Sinensis extract.
(6) Rhizoma Alismatis taking described parts by weight cleans, dry, pulverize after cross 20 mesh sieves, carry with 9 times amount 85 % alcohol refluxs
Taking 2 times, each 1 h, united extraction liquid, filter, filtrate is concentrated to give primary extract, by nonpolar macroporous for primary extract styrene type tree
Fat adsorbs, and purifies after washing post with 7 times of column volume 60 % ethanol solution eluting with 2 times of column volumes, collection eluent recycling design,
Concentrate drying, obtains Rhizoma Alismatis extract.
(7) take above-mentioned prepare Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract
Thing and Rhizoma Alismatis extract, be equipped with corresponding additive of tablet, make tablet.
Embodiment 6
For treating the Chinese medicine of gout and hyperuricemia, it is made up by described parts by weight of following crude drug: Cortex Phellodendri 50
Part, Radix Sophorae Flavescentis 50 parts, Radix Stephaniae Tetrandrae 35, Rhizoma Alismatis 40 parts, Rhizoma Polygoni Cuspidati 40 parts, Radix Angelicae Sinensis 60 parts.
Its preparation method is as follows:
(1) Cortex Phellodendri taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, carry with 7 times of medical material amount 75 % alcohol refluxs
Taking 2 times, each 2 hours, collect extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, by weak for first extract styrene type
Polarity or non-polar macroporous resin absorption, with after 2 times of column volumes washing posts with 7 times of column volume 50 % ethanol solution eluting, collection
Eluent recycling design, concentrate drying, obtain Cortex Phellodendri extract；
(2) Radix Sophorae Flavescentis taking described parts by weight cleans, dry, pulverize after cross 60 mesh sieves, carry with 8 times of medical material amount 75 % alcohol refluxs
Take 3 times, each 1 hour, and and extracting solution, filter, filtrate is concentrated into thick shape, obtains just extract, will first extract, first extract is used
Styrene type low pole or non-polar macroporous resin absorption, wash after posts with 4 times of column volume 80 % ethanol solution with 2 times of column volumes
Eluting, collects eluent recycling design, is dried, obtains Radix Sophorae Flavescentis extract.
(3) Radix Stephaniae Tetrandrae taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, with the ethanol of 8 times of medical material amounts 75%
Reflux, extract, 2 times, each 2.5 hours, the filtrate of filtration and filtering residue, reflux, extract, that filtering residue is added water 2 times, collect extracting solution, close
And filtrate and extracting solution, it is concentrated to give just extract, by first extract styrene type low pole or non-polar macroporous resin suction
Attached (medicinal liquid resin than for 1:1), returns with 8 times of column volume 90 % ethanol solution eluting, collection eluent after 3 times of column volumes washing posts
Receive solvent, be dried, obtain Radix Stephaniae Tetrandrae extract.
(4) Rhizoma Polygoni Cuspidati taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 9 times of medical material amount 75 % ethanol
Stream extracts 1 time, each 1.5 h, filter, obtains filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filter
Liquid, concentrates, is drying to obtain Rhizoma Polygoni Cuspidati extract.
(5) Radix Angelicae Sinensis taking described parts by weight cleans, dry, pulverize after cross 40 mesh sieves, return with 9 times of medical material amount 70 % ethanol
Stream extracts 1 time, extracts 2 hours, united extraction liquid, filters, and filtrate is concentrated to give primary extract, and primary extract is used styrene type low pole
Macroporous resin adsorption, purifies with 3 times of column volumes and returns with 9 times of column volume 60 % ethanol solution eluting, collection eluent after washing post
Receive solvent, concentrate drying, obtain Radix Angelicae Sinensis extract.
(6) Rhizoma Alismatis taking described parts by weight cleans, dry, pulverize after cross 20 mesh sieves, carry with 9 times amount 85 % alcohol refluxs
Taking 2 times, each 1 h, united extraction liquid, filter, filtrate is concentrated to give primary extract, by nonpolar macroporous for primary extract styrene type tree
Fat adsorbs, and purifies after washing post with 7 times of column volume 60 % ethanol solution eluting with 3 times of column volumes, collection eluent recycling design,
Concentrate drying, obtains Rhizoma Alismatis extract.
(7) take above-mentioned prepare Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract
Thing and Rhizoma Alismatis extract, be equipped with corresponding additive of tablet, make pill.
Embodiment 7
For treating the Chinese medicine of gout and hyperuricemia, it is to be mixed by described parts by weight by following active component:
Cortex Phellodendri extract 1 part, Radix Sophorae Flavescentis extract 2 parts, Radix Stephaniae Tetrandrae extract 12 parts, Rhizoma Polygoni Cuspidati extract 18 parts, Radix Angelicae Sinensis extract 24 parts
With Rhizoma Alismatis extract 24 parts.
Wherein Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract and Rhizoma Alismatis are extracted
The preparation method of thing is identical with embodiment one.
Embodiment 8
For treating the Chinese medicine of gout and hyperuricemia, it is to be mixed by described parts by weight by following active component:
Cortex Phellodendri extract 9 parts, Radix Sophorae Flavescentis extract 12 parts, Radix Stephaniae Tetrandrae extract 2 parts, Rhizoma Polygoni Cuspidati extract 3 parts, Radix Angelicae Sinensis extract 2 parts and pool
Rush down extract 2 parts.
Wherein Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract and Rhizoma Alismatis are extracted
The preparation method of thing is identical with embodiment two.
Embodiment 9
For treating the Chinese medicine of gout and hyperuricemia, it is to be mixed by described parts by weight by following active component:
Cortex Phellodendri extract 6 parts, Radix Sophorae Flavescentis extract 6 parts, Radix Stephaniae Tetrandrae extract 8 parts, Rhizoma Polygoni Cuspidati extract 10 parts, Radix Angelicae Sinensis extract 12 parts and Rhizoma Alismatis
Extract 12 parts.
Wherein Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract and Rhizoma Alismatis are extracted
The preparation method of thing is identical with embodiment three.
Embodiment 10
For treating the Chinese medicine of gout and hyperuricemia, it is to be mixed by described parts by weight by following active component:
Cortex Phellodendri extract 3 parts, Radix Sophorae Flavescentis extract 4 parts, Radix Stephaniae Tetrandrae extract 4 parts, Rhizoma Polygoni Cuspidati extract 4 parts, Radix Angelicae Sinensis extract 6 parts and Rhizoma Alismatis carry
Take thing 4 parts.
Wherein Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract and Rhizoma Alismatis are extracted
The preparation method of thing is identical with embodiment four.
Embodiment 11
For treating the Chinese medicine of gout and hyperuricemia, it is to be mixed by described parts by weight by following active component:
Cortex Phellodendri extract 9 parts, Radix Sophorae Flavescentis extract 12 parts, Radix Stephaniae Tetrandrae extract 12 parts, Rhizoma Polygoni Cuspidati extract 9 parts, Radix Angelicae Sinensis extract 12 parts and pool
Rush down extract 12 parts.
Wherein Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract and Rhizoma Alismatis are extracted
The preparation method of thing is identical with embodiment five.
Embodiment 12
For treating the Chinese medicine of gout and hyperuricemia, it is to be mixed by described parts by weight by following active component:
Cortex Phellodendri extract 1 part, Radix Sophorae Flavescentis extract 2 parts, Radix Stephaniae Tetrandrae extract 2 parts, Rhizoma Polygoni Cuspidati extract 2 parts, Radix Angelicae Sinensis extract 3 parts and Rhizoma Alismatis carry
Take thing 2 parts.
Wherein Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract and Rhizoma Alismatis are extracted
The preparation method of thing is identical with embodiment four.
The embodiment of the present invention 2,6,10 gained is for treating the pharmacological research of the Chinese medicine of gout and hyperuricemia:
Internal inhibiting hyperuricemia pharmacodynamic experiment
One, experiment material
1, laboratory animal: healthy male mice in kunming, body weight is (20+2) g, and long-living Bioisystech Co., Ltd carries by Liaoning
Confession, the quality certification number is: SCXK2014-2002.
2, medicine: Rhizoma Coptidis, Fructus Evodiae, Herba Leonuri, Rhizoma Corydalis, Herba Lophatheri, Semen Coicis extract are made according to a conventional method；Place
1 group, side is prepared from by embodiment 2；Prescription 2 groups is prepared from by embodiment 6；Prescription 3 groups is prepared from by embodiment 10；
Reagent: uric acid, blood urea nitrogen test kit are purchased from Hui Li bio tech ltd, Changchun；It is permanent that Oteracil Potassium is purchased from Sheng sky, Hubei
Wound bio tech ltd.
Two, mice Oteracil Potassium hyperuricemia model is set up
Healthy male mouse of kunming, every day, gavage gave Oteracil Potassium (450mg/kg), continuous 7 days, first 1 hour of each modeling,
Prohibit water non-fasting.
Three, the test of pesticide effectiveness in prescription anti-mouse hyperuricemia body
Allopurinol is the Western medicine that can effectively reduce Uric Acid Content, therefore selects allopurinol as Western medicine positive control drug, experiment
It is set to blank group, model group, allopurinol group, prescription 1 group, prescription 2 groups and prescription 3 groups.Except blank group gives commensurability life
Outside reason saline, remaining group mice all gives Oteracil Potassium and builds hyperuricemia model.Positive drug group gives allopurinol (5
Mg/kg), other each administration groups eat dosage by the people of States Pharmacopoeia specifications and are converted into mice required dosage gastric infusion.Experiment is carried out
During by the 6th day, put in metabolic cage by often organizing mice, collect twenty-four-hour urine liquid.Test the 7th day, after being administered 2 hours, aseptic pluck
Except eyeball takes blood and nephridial tissue, separate serum, save backup.
Three, elisa RNA isolation kit, biochemistry analyzer method measure uric acid and urea nitrogen content
It is respectively adopted elisa uric acid reagent box and biochemistry analyzer measures the uric acid level often organized in Mouse Blood, urine (according to reagent
Operating procedure in box description is carried out).The results are shown in Table 1.
Table 1 uric acid and urea nitrogen content measurement result
。
As shown in Table 1, prescription administration group and the Mouse Blood uric acid of allopurinol administration group, acid content of urinating are all than model group
Few, illustrate that the prescription of present embodiment has preferable uric acid resisting effect, thus reach significantly to prevent and treat the drug effect of gout.
Claims (10)
1. for treating the Chinese medicine of gout and hyperuricemia, it is characterised in that it is by described parts by weight by following crude drug
Make: Cortex Phellodendri 10-100 parts, Radix Sophorae Flavescentis 14-88 parts, Radix Stephaniae Tetrandrae 9-60 parts, Rhizoma Alismatis 7-86 parts, Rhizoma Polygoni Cuspidati 11.5-70
Part, Radix Angelicae Sinensis 10-130 parts.
Chinese medicine for treating gout and hyperuricemia the most according to claim 1, it is characterised in that it is by following
Crude drug is made by described parts by weight: Cortex Phellodendri 40-60 parts, Radix Sophorae Flavescentis 45-60 parts, Radix Stephaniae Tetrandrae 30-40 parts, Rhizoma Alismatis 35-
45 parts, Rhizoma Polygoni Cuspidati 35-45 parts, Radix Angelicae Sinensis 50-70 parts.
3. the preparation method of the Chinese medicine for treating gout and hyperuricemia described in claim 1 or 2, it is characterised in that step
Rapid as follows:
(1) take and meet the Cortex Phellodendri of described parts by weight, with the 75 % alcohol reflux 2 times of 6-8 times of medical material amounts, each 1-
2 hours, united extraction liquid, to filter, filtrate is concentrated to give primary extract；By primary extract styrene type low pole or nonpolar macroporous tree
Fat adsorbs, and purifies after washing post with 6-8 times of column volume 50 % ethanol solution eluting with 1-3 times of column volume, collection eluent
Recycling design, concentrate drying, obtain Cortex Phellodendri extract, standby；
(2) take and meet the Radix Sophorae Flavescentis of described parts by weight, with 60-80 % alcohol reflux 3 times of 6-10 times of medical material amounts, often
Secondary 1 hour, united extraction liquid, to filter, filtrate is concentrated to give primary extract, primary extract styrene type non-polar macroporous resin is inhaled
Attached, purify with 1-3 times of column volume and reclaim with 3-5 times of column volume 80 % ethanol solution eluting, collection eluent after washing post
Solvent, concentrate drying, obtain Radix Sophorae Flavescentis extract, standby；
(3) take and meet the Radix Stephaniae Tetrandrae of described parts by weight, with the 70 % alcohol reflux 1-2 time of 7-9 times of medical material amounts, every time
2-3 h, filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate, at the beginning of being concentrated to give
Extract；By primary extract by styrene type low pole macroporous resin adsorption and with 8 times of column volume 90 % ethanol solution eluting, collect
Eluent recycling design, is drying to obtain Radix Stephaniae Tetrandrae extract, standby；
(4) take and meet the Rhizoma Polygoni Cuspidati of described parts by weight, with 70-80 % alcohol reflux 1-2 of 8-10 times of medical material amounts
Secondary, each 1.5 h, filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate, dense
Contract, be drying to obtain Rhizoma Polygoni Cuspidati extract, standby；
(5) take and meet the Radix Angelicae Sinensis of described parts by weight, with 60-80 % alcohol reflux 1 time of 8-10 times of medical material amounts, often
Secondary 2 hours, united extraction liquid, to filter, filtrate is concentrated to give primary extract, is inhaled by primary extract styrene type low pole macroporous resin
Attached, purify with 1-3 times of column volume and reclaim with 8-10 times of column volume 60 % ethanol solution eluting, collection eluent after washing post
Solvent, concentrate drying, obtain Radix Angelicae Sinensis extract, standby；
(6) take and meet the Rhizoma Alismatis of described parts by weight, with 8-10 times amount 80-90 % alcohol reflux 2 times, each 1 h,
United extraction liquid, filters, and filtrate is concentrated to give primary extract, primary extract styrene type non-polar macroporous resin is adsorbed, with 1-3
With 6-8 times of column volume 60 % ethanol solution eluting after times column volume purification washing post, collect eluent recycling design, concentrate and do
Dry, obtain Rhizoma Alismatis extract, standby；
(7) Cortex Phellodendri extract, Radix Sophorae Flavescentis extract, Radix Stephaniae Tetrandrae extract, Rhizoma Polygoni Cuspidati extract, Radix Angelicae Sinensis extract and Rhizoma Alismatis extract are taken,
It is equipped with corresponding adjuvant and makes capsule, tablet, granule, pill peroral dosage form.
4. for treating the Chinese medicine of gout and hyperuricemia, it is characterised in that it is by described parts by weight by following crude drug
It is mixed with and forms: Cortex Phellodendri extract 1-9 part, Radix Sophorae Flavescentis extract 2-12 parts, Radix Stephaniae Tetrandrae extract 2-12 parts, Rhizoma Polygoni Cuspidati carry
Take thing 3-18 parts, Radix Angelicae Sinensis extract 2-24 parts and Rhizoma Alismatis extract 2-24 parts.
Chinese medicine for treating gout and hyperuricemia the most according to claim 4, it is characterised in that described Cortex Phellodendri
The preparation method of extract: by the Cortex Phellodendri 75 % alcohol reflux 2 times of 6-8 times of medical material amounts, each 1-2 hour, close
And extracting solution, to filter, filtrate is concentrated to give primary extract；Primary extract styrene type low pole or non-polar macroporous resin are adsorbed,
Purify after washing post with 6-8 times of column volume 50 % ethanol solution eluting with 1-3 times of column volume, collect eluent recovery molten
Agent, concentrate drying, obtain Cortex Phellodendri extract.
Chinese medicine for treating gout and hyperuricemia the most according to claim 4, it is characterised in that described Radix Sophorae Flavescentis
The preparation method of extract: by Radix Sophorae Flavescentis 60-80 % alcohol reflux 3 times of 6-10 times of medical material amounts, each 1 hour,
United extraction liquid, filters, and filtrate is concentrated to give primary extract, primary extract styrene type non-polar macroporous resin is adsorbed, with 1-3
With 3-5 times of column volume 80 % ethanol solution eluting after times column volume purification washing post, collect eluent recycling design, concentrate and do
Dry, obtain Radix Sophorae Flavescentis extract.
Chinese medicine for treating gout and hyperuricemia the most according to claim 4, it is characterised in that described Radix Stephaniae Tetrandrae
The preparation method of extract: by the Radix Stephaniae Tetrandrae 70 % alcohol reflux 1-2 time of 7-9 times of medical material amounts, each 2-3 h,
Filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate, it is concentrated to give just extract；
By primary extract by styrene type low pole macroporous resin adsorption and with 8 times of column volume 90 % ethanol solution eluting, collect eluent
Recycling design, is drying to obtain Radix Stephaniae Tetrandrae extract.
Chinese medicine for treating gout and hyperuricemia the most according to claim 4, it is characterised in that described Rhizoma Polygoni Cuspidati
The preparation method of extract: Rhizoma Polygoni Cuspidati is drunk 70-80 % alcohol reflux 1-2 time of 8-10 times of medical material amounts, every time
1.5 h, filter, obtain filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate, concentrate, be dried
Obtain Rhizoma Polygoni Cuspidati extract.
Chinese medicine for treating gout and hyperuricemia the most according to claim 4, it is characterised in that described Radix Angelicae Sinensis
The preparation method of extract: by Radix Angelicae Sinensis 60-80 % alcohol reflux 1 time of 8-10 times of medical material amounts, each 2 hours,
United extraction liquid, filters, and filtrate is concentrated to give primary extract, by primary extract styrene type low pole macroporous resin adsorption, with 1-3
With 8-10 times of column volume 60 % ethanol solution eluting after times column volume purification washing post, collect eluent recycling design, concentration
It is dried, obtains Radix Angelicae Sinensis extract；The preparation method of described Rhizoma Alismatis extract: Rhizoma Alismatis decoction pieces is cleaned, dries, pulverized and sieved,
With 8-10 times amount 80-90 % alcohol reflux 2 times, each 1 h, united extraction liquid, filtering, filtrate is concentrated to give and just carries
Thing, adsorbs primary extract styrene type non-polar macroporous resin, purifies with 1-3 times of column volume and washes after post with 6-8 times
Column volume 60 % ethanol solution eluting, collects eluent recycling design, concentrate drying, obtains Rhizoma Alismatis extract.
Chinese medicine for treating gout and hyperuricemia the most according to claim 4, it is characterised in that described Cortex Phellodendri
The preparation method of extract: by Cortex Phellodendri 75% alcohol reflux 2 times of 6-8 times of medical material amount, each 1-2 hour, united extraction
Liquid, filters, and filtrate is concentrated to give primary extract, adsorbs, primary extract styrene type low pole or non-polar macroporous resin with 1-3 times
With 6-8 times of column volume 50% ethanol solution eluting after column volume purification washing post, collection eluent recycling design, concentrate drying, i.e.
Obtain Cortex Phellodendri extract；The preparation method of Radix Sophorae Flavescentis extract: by the Radix Sophorae Flavescentis 60-80% alcohol reflux 3 of 6-10 times of medical material amount
Secondary, each 1 hour, united extraction liquid, to filter, filtrate is concentrated to give primary extract, by nonpolar macroporous for primary extract styrene type tree
Fat adsorbs, and purifies after washing post with 3-5 times of column volume 80% ethanol solution eluting with 1-3 times of column volume, collects eluent and reclaim molten
Agent, concentrate drying, obtain Radix Sophorae Flavescentis extract；The preparation method of Radix Stephaniae Tetrandrae extract: by Radix Stephaniae Tetrandrae 70% ethanol of 7-9 times of medical material amount
Reflux, extract, 1-2 time, each 2-3h, filters, obtains filtrate and filtering residue；By filtering residue use water reflux, extract, 2 times, united extraction liquid and upper
State filtrate, be concentrated to give just extract, by primary extract by styrene type low pole macroporous resin adsorption and by 8 times of column volume 90% second
Alcoholic solution eluting, collects eluent recycling design, is drying to obtain Radix Stephaniae Tetrandrae extract；The preparation method of Rhizoma Polygoni Cuspidati extract: by Rhizoma Polygoni Cuspidati
With 70-80% alcohol reflux 1-2 time of 8-10 times of medical material amount, each 1.5h, filter, obtain filtrate and filtering residue, by filtering residue water
Reflux, extract, 2 times, united extraction liquid and above-mentioned filtrate, concentrate, be drying to obtain Rhizoma Polygoni Cuspidati extract；The preparation side of Radix Angelicae Sinensis extract
Method: by the Radix Angelicae Sinensis 60-80% alcohol reflux 1 time of 8-10 times of medical material amount, each 2 hours, united extraction liquid, filters, filtrate
It is concentrated to give primary extract, by primary extract styrene type low pole macroporous resin adsorption, purifies with 1-3 times of column volume and use after washing post
8-10 times of column volume 60% ethanol solution eluting, collects eluent recycling design, concentrate drying, obtains Radix Angelicae Sinensis extract；Rhizoma Alismatis carries
Take the preparation method of thing: by Rhizoma Alismatis 8-10 times amount 80-90% alcohol reflux 2 times, each 1h, united extraction liquid, filter,
Filtrate is concentrated to give primary extract, primary extract styrene type non-polar macroporous resin is adsorbed, and purifies with 1-3 times of column volume and washes post
Afterwards with 6-8 times of column volume 60% ethanol solution eluting, collect eluent recycling design, concentrate drying, obtain Rhizoma Alismatis extract.
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Cited By (1)
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| CN111419885A (en) * | 2020-03-10 | 2020-07-17 | 华中农业大学 | Inonotus obliquus extract with uric acid reducing effect |
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