CN105993938B - A kind of method using the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement - Google Patents
A kind of method using the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement Download PDFInfo
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- CN105993938B CN105993938B CN201610321546.6A CN201610321546A CN105993938B CN 105993938 B CN105993938 B CN 105993938B CN 201610321546 A CN201610321546 A CN 201610321546A CN 105993938 B CN105993938 B CN 105993938B
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- Prior art keywords
- pseudo
- ginseng
- resting bud
- gibberellin
- vitro
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Abstract
The invention discloses a kind of method using the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement, including taking field pseudo-ginseng, resting bud is cut, cleaning, and remove the tissue that surface has been ftractureed, then 1 2min is soaked with 70% ethanol, with sterile water wash, again 8 10min are soaked with 10% liquor natrii hypochloritis, with aseptic water washing, resting bud after processing is colonized on the MS culture mediums containing gibberellin, illumination cultivation, emerged in 67 days, it is transferred to again in the MS culture mediums containing the BA of the basic element of cell division 6 and auxin IAA, the pseudo-ginseng seedling grown breaks up within 3 weeks takes root, it is transplanted to big Tanaka and carries out transplanting plantation.The present invention using in vitro Propogation and culture pseudo-ginseng seedling robust plant not easy infection pest and disease damage, overcome that Panax notoginseng seeds nursery germination rate is low and seed is difficult to ensure the problems such as depositing, the seasonal dependence of notoginseng planting is broken, has promoted the annual production of pseudo-ginseng, there is good economic benefit and application value.
Description
Technical field
The present invention relates to notoginseng planting technical field, and in particular to one kind is gone out using the in vitro pseudo-ginseng resting bud of gibberellin inducement
The method of seedling.
Background technology
Pseudo-ginseng category Araliaceae herbaceos perennial, its cauline leaf, which is spent, can be used as medicine, warm-natured, bitter, have dissipate stasis of blood hemostasis, disappear
The effect of swollen analgesic, available for treating various hemorrhagic conditions and swelling and pain illness, the flavone compound in pseudo-ginseng has and improves the heart
Flesh blood supply, increase blood vessel elasticity, coronary artery dilator the effect of, pseudo-ginseng has reducing blood lipid, to coronary heart diseases and angina pectoris have prevention and
Therapeutic action, therefore, field of medicaments is increasing for the demand of pseudo-ginseng, and the market supply of pseudo-ginseng is slower and slower, this master
It is Panax notoginseng Growth cycle length to want reason, and mostly using seed seedling-raising, cultivation management technology of the prior art is poor, on the one hand often
The serious economic loss caused by the storage of seeds is improper, the germination rate and survival rate of another aspect Panax notoginseng seeds are relatively low.
In recent years, also have tried to use pseudo-ginseng resting bud and carry out nursery, but because pseudo-ginseng is that thing is taken root in contracting, root tuber has bright
Aobvious dormant trait, pseudo-ginseng seedling are sprouted from Dormant bud development to complete, the dormancy time for about needing 90 days, and need certain low temperature
(10 DEG C of ＜) can just break, less so as to carry out the relevant report of nursery in actual production using resting bud.
The content of the invention
Object of the present invention is to provide a kind of method using the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement, emergence
Fast and emergence rate is high.
To realize object above, the present invention is achieved by the following technical programs：
A kind of method using the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement, comprise the following steps：
1) field pseudo-ginseng is taken, resting bud is cut, is cleaned, and remove the tissue that surface has been ftractureed；
2) resting bud after step 1) processing with sterile water wash 3-4 times, then is used with 70% ethanol immersion 1-2min
10% liquor natrii hypochloritis soaks 8-10min, with aseptic water washing 3-4 times；
3) resting bud after step 2) processing is colonized on the MS culture mediums containing gibberellin, illumination cultivation, in 6-7 days
Emergence, obtains pseudo-ginseng seedling；
4) the pseudo-ginseng seedling of step 3) is transferred to containing the 1mg/L-5mg/L basic elements of cell division 6-BA and 0.5mg/L-1.5mg/
In L auxin IAA MS culture mediums, the pseudo-ginseng seedling that is grown breaks up within 3 weeks takes root；
5) the pseudo-ginseng seedling after step 4) is taken root is transplanted to big Tanaka and carries out transplanting plantation.
Preferably, pseudo-ginseng is annual or biennial pseudo-ginseng in the step 1).
Preferably, illumination cultivation temperature is 20-25 DEG C, light application time 12h/d, intensity of illumination 2500- in the step 3)
2800Lx。
Preferably, the concentration of gibberellin is 0.1mg/L-100mg/L in the step 3).
Beneficial effect of the present invention：The present invention is quickly emerged in one week using the in vitro pseudo-ginseng resting bud of gibberellin inducement, gram
Pseudo-ginseng is taken in winter because dormancy is without the problem of emergence, emergence is fast, and emergence rate is more than 98%, shortens breeding week
Phase, the use of agriculture chemical is reduced, save cost of labor and mitigate environmental pollution.The present invention uses the pseudo-ginseng of in vitro Propogation and culture
Seedling robust plant not easy infection pest and disease damage, overcomes that Panax notoginseng seeds nursery germination rate is low and seed is difficult to ensure the problems such as depositing, and breaks
The seasonal dependence of notoginseng planting, promotes the annual production of pseudo-ginseng, has good economic benefit and application value.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1：The in vitro resting bud of pseudo-ginseng growing state (wherein, A in the MS culture mediums containing different gibberellin concentration：Be free of
Gibberellin, B：0.1mg/L, C：10mg/L；D：30mg/L；E：50mg/L；F：100mg/L).
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described, it is clear that described embodiment is
Part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art
The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
Embodiment：
A kind of method using the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement, comprise the following steps：
1) take field annual or biennial pseudo-ginseng, resting bud is cut, clean, and remove the group that surface has been ftractureed
Knit；
2) resting bud after step 1) processing with sterile water wash 3-4 times, then is used with 70% ethanol immersion 1-2min
10% liquor natrii hypochloritis soaks 8-10min, with aseptic water washing 3-4 times；
3) resting bud after step 2) processing is colonized on the MS culture mediums containing 0.1mg/L-100mg/L gibberellin,
Illumination cultivation, cultivation temperature are 20-25 DEG C, light application time 12h/d, intensity of illumination 2500-2800Lx, are emerged in 6-7 days, obtain three
Seven seedlings；
4) the pseudo-ginseng seedling of step 3) is transferred to containing the 1mg/L-5mg/L basic elements of cell division 6-BA and 0.5mg/L-1.5mg/
In L auxin IAA MS culture mediums, the pseudo-ginseng seedling that is grown breaks up within 3 weeks takes root；
5) the pseudo-ginseng seedling after step 4) is taken root is transplanted to big Tanaka and carries out transplanting plantation.
Induction emergence will be carried out to pseudo-ginseng resting bud by the above process, in vitro resting bud is soaked into 90s with 70% ethanol
Afterwards, with sterile water wash 3-4 times, then with 10% liquor natrii hypochloritis 10min is soaked, with aseptic water washing 3-4 times, then by dormancy
Bud is colonized on the MS culture mediums containing 0mg/L-100mg/L gibberellin, illumination cultivation, and cultivation temperature is 20-25 DEG C, during illumination
Between 12h/d, intensity of illumination 2500-2800Lx, keep rearing condition it is consistent, from table 1 it follows that without gibberellin MS training
Supporting base seedling raise period needs 15-20 days, also substantially reduces the seedling-growing time of resting bud even if the gibberellin of low concentration, and emerge
Rate also increases, and seedling body stalwartness situation low concentration will get well in the gibberellin of high concentration, and emergence significantly improves.From Fig. 1
As can be seen that pseudo-ginseng resting bud seedling-growing time and healthy and strong situation are optimal when gibberellin concentration is 50mg/L in MS cultures.
Influence of the gibberellin concentration of table 1 to pseudo-ginseng resting bud emergence situation
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
The present invention is described in detail, it will be understood by those within the art that：It still can be to foregoing each implementation
Technical scheme described in example is modified, or carries out equivalent substitution to which part technical characteristic；And these modification or
Replace, the essence of appropriate technical solution is departed from the spirit and scope of various embodiments of the present invention technical scheme.
Claims (2)
- A kind of 1. method using the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement, it is characterised in that comprise the following steps：1) field pseudo-ginseng is taken, resting bud is cut, is cleaned, and remove the tissue that surface has been ftractureed；2) by the resting bud after step 1) processing with 70% ethanol immersion 1-2min, with sterile water wash 3-4 times, then with 10% Liquor natrii hypochloritis soaks 8-10min, with aseptic water washing 3-4 times；3) resting bud after step 2) processing is colonized on the MS culture mediums of the only gibberellin containing 30-100mg/L, illumination training Support, emerged in 6-7 days, obtain pseudo-ginseng seedling, wherein, illumination cultivation temperature is 20-25 DEG C, light application time 12h/d, intensity of illumination 2500-2800Lx；4) the pseudo-ginseng seedling of step 3) is transferred to and only contains the 1mg/L-5mg/L basic elements of cell division 6-BA and 0.5mg/L-1.5mg/L In auxin IAA MS culture mediums, the pseudo-ginseng seedling that is grown breaks up within 3 weeks takes root；5) the pseudo-ginseng seedling after step 4) is taken root is transplanted to big Tanaka and carries out transplanting plantation.
- 2. the method for the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement is utilized as claimed in claim 1, it is characterised in that described Pseudo-ginseng is annual or biennial pseudo-ginseng in step 1).
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| CN105993938A CN105993938A (en) | 2016-10-12 |
| CN105993938B true CN105993938B (en) | 2018-01-02 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106718891A (en) * | 2016-12-04 | 2017-05-31 | 淄博精诚专利信息咨询有限公司 | A kind of pseudo-ginseng asexual reproduction method of efficient stable |
| CN112616672A (en) * | 2020-12-31 | 2021-04-09 | 云南农业大学 | Method for directly inducing seedling emergence by utilizing stem segments of panax notoginseng |
| CN112640783A (en) * | 2020-12-31 | 2021-04-13 | 云南农业大学 | Method for directly inducing seedling emergence by using pseudo-ginseng leaves |
| CN112877355A (en) * | 2021-01-22 | 2021-06-01 | 杜云龙 | Method for expressing notoginsenoside by using tobacco |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104813938A (en) * | 2015-05-14 | 2015-08-05 | 黄振忠 | Panax notoginseng tissue culture seedling raising method |
| CN104823854A (en) * | 2015-05-15 | 2015-08-12 | 黄振忠 | Primary culture medium special for panax notoginseng tissue culture seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104813938A (en) * | 2015-05-14 | 2015-08-05 | 黄振忠 | Panax notoginseng tissue culture seedling raising method |
| CN104823854A (en) * | 2015-05-15 | 2015-08-12 | 黄振忠 | Primary culture medium special for panax notoginseng tissue culture seedlings |
Non-Patent Citations (1)
| Title |
|---|
| 种子处理及GA3浓度对诱导三七实生苗影响研究;孙玉琴等;《特产研究》;20131231(第4期);第48页左栏第3-4段、右栏第1-2段，第49页左栏第2段 * |
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