CN104823854A - Primary culture medium special for panax notoginseng tissue culture seedlings - Google Patents
Primary culture medium special for panax notoginseng tissue culture seedlings Download PDFInfo
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- CN104823854A CN104823854A CN201510245183.8A CN201510245183A CN104823854A CN 104823854 A CN104823854 A CN 104823854A CN 201510245183 A CN201510245183 A CN 201510245183A CN 104823854 A CN104823854 A CN 104823854A
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- panax notoginseng
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- ginseng
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Abstract
The invention discloses a primary culture medium special for tissue culture seedlings of a precious medicinal material namely panax notoginseng, and belongs to the technical field of tissue culture seedling raising. By adopting the primary culture medium, the problem that the multiple shoot induction rate is low (less than 50%) in a panax notoginseng tissue culture process performed by people can be effectively solved. The primary culture medium consists of an MS culture medium, benzyl adenine (BA), naphthylacetic acid (NAA), cane sugar and agar. By adopting the primary culture medium disclosed by the invention, the multiple shoot induction rate can reach 72-93%, the implementation of panax notoginseng tissue culture seedling raising can be successfully ensured, and the requirements of artificial large-area cultivation for panax notoginseng seedlings can be met.
Description
Technical field
The present invention relates to a kind of Initial culture base, be specifically related to a kind of special Initial culture base of pseudo-ginseng tissue cultivating and seedling, belong to tissue cultivating and seedling technical field.
Background technology
Pseudo-ginseng, Latin is called: Panax notoginseng (Burk.) F.H.Chen ex G.Y.WuetK.M.Feng, also claims pseudo-ginseng, is Araliaceae herbaceos perennial.Be used as medicine with root, root-like stock, the raw product stasis of blood of faling apart is stopped blooding, Repercusion analgesia, control spitting of blood, haematemesis, bleeding from five sense organs or subcutaneous tissue, have blood in stool, the prolapse of uterus, chest ventral spine pain, traumatism and bleeding, treating swelling and pain by traumatic injury; Ripe product can be enriched blood and be invigorated blood circulation, control lose blood, anaemia.Flower, leaf also can be done medicinal, and flower has the effect of heat-clearing, flat liver, step-down.According to modern clinic newly use, pseudo-ginseng also can treat serious hepatitis, high fat of blood, coronary heart disease, UGB, craniocerebral trauma, hyphema, and has anti-cancer and kill cancer action.
Pseudo-ginseng is rare traditional Chinese medicine, Ye Shi China tradition parts of generic medicinal plants, except for domestic medicinal except, or one of large export-oriented commodity of China, is widely used in the industry of medical industry, daily-use chemical industry, food industry and cosmetics, existing national annual requirement about 420 kilograms, also export 180 kilograms, along with the development of medical and health cause and the raising of people's living standard, the requirement of pseudo-ginseng also will increase, and can greatly develop notoginseng planting.And carry out pseudo-ginseng artificial planting, first pseudo-ginseng nursery problem will be solved, carrying out tissue cultivating and seedling without coagulating is the most effective pseudo-ginseng seedling-cultivating method, and tissue cultivating and seedling most critical prepares medium, medium comprises Initial culture base, subculture medium and root media, wherein the effect of Initial culture base makes aseptic seedling induce Multiple Buds, and present people carry out pseudo-ginseng tissue cultures inducing clumping bud rate less than 50%, are the main causes causing the failure of pseudo-ginseng tissue cultures.
Summary of the invention
goal of the invention: for prior art Problems existing, the invention provides the Initial culture base that a kind of pseudo-ginseng plantlet in vitro is special, improve pseudo-ginseng tissue cultures inducing clumping bud rate, guarantee that pseudo-ginseng tissue cultivating and seedling obtains effect.
technical scheme: the special Initial culture base of a kind of pseudo-ginseng plantlet in vitro of the present invention, this Initial culture base is the MS medium containing concentration to be the benzyl purine (BA) of 1.5 ~ 2.4 mg/L and concentration the be naa (NAA) of 0.1 ~ 0.3mg/L, that is medium based on MS medium, then to MS basal medium, to add concentration be the benzyl purine (BA) of 1.5 ~ 2.4 mg/L and concentration is the naa (NAA) of 0.1 ~ 0.3mg/L, wherein the preferred concentration of benzyl purine (BA) is 2.0mg/L, the preferred concentration of naa (NAA) is 0.2mg/L, add the sucrose of 2% and the agar of 0.5% simultaneously, medium temperature keeps 24 DEG C ~ 26 DEG C, pH value 5.9 ~ 6.2, illumination 1600 ~ 1900Lx, 12 hours every days.
beneficial effect: compared with prior art, its beneficial effect is in the present invention:
The special Initial culture base of pseudo-ginseng tissue cultures of the present invention, pseudo-ginseng aseptic seedling can be made to form Multiple Buds, and inducing clumping bud rate reaches 72% ~ 93%, and draws materials conveniently, guarantees that pseudo-ginseng tissue cultivating and seedling succeeds.
Embodiment
Below in conjunction with concrete example of implementing, technical solution of the present invention is described in detail.
Embodiment: the experiment of pseudo-ginseng aseptic seedling cultivated by the Initial culture base containing hormon.
Panax notoginseng seeds is placed in 0.2% mercuric chloride solution soaking disinfection 15 minutes, superclean bench embathes 5 times with sterile water, embathes 3 minutes at every turn, then by planting seed in medium, cultivate under Indoor Natural light scattering, namely obtain pseudo-ginseng aseptic seedling.
After pseudo-ginseng aseptic seedling grows to 5 ~ 6 centimetres, cut the top leaf stem section of top 2.6 ~ 2.9 centimeter length, 1. access is numbered 2. Initial culture base 3. respectively, namely cultivate had Multiple Buds to be formed after 28 days, calculate different Initial culture bases, inducing clumping bud rate is also different, and result is as following table.Visible cell mitogen BA and growth hormone NAA concentration are the factors determining that Multiple Buds is formed, the higher formation that may not be conducive to Multiple Buds of BA and NAA consumption.
Subordinate list: the Initial culture base of hormon concentration and inducing clumping bud rate thereof
Claims (3)
1. the special Initial culture base of pseudo-ginseng plantlet in vitro, this special Initial culture base comprises MS medium, benzyl purine (BA), naa (NAA), sucrose and agar, it is characterized in that the concentration of benzyl purine (BA) is 1.5 ~ 2.4 mg/L, the concentration of naa (NAA) is 0.1 ~ 0.3mg/L, the concentration of sucrose is 2%, and the concentration of agar is 0.5.
2. the special Initial culture base of pseudo-ginseng plantlet in vitro according to claim 1, it is characterized in that the concentration of described benzyl purine (BA) is 2.0 mg/L, the concentration of naa (NAA) is 0.2mg/L.
3. the special Initial culture base of pseudo-ginseng plantlet in vitro according to claim 2, is characterized in that described special Initial culture Ji Wendu keeps 24 DEG C ~ 26 DEG C, pH value 5.9 ~ 6.2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510245183.8A CN104823854A (en) | 2015-05-15 | 2015-05-15 | Primary culture medium special for panax notoginseng tissue culture seedlings |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510245183.8A CN104823854A (en) | 2015-05-15 | 2015-05-15 | Primary culture medium special for panax notoginseng tissue culture seedlings |
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| CN104823854A true CN104823854A (en) | 2015-08-12 |
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| CN201510245183.8A Pending CN104823854A (en) | 2015-05-15 | 2015-05-15 | Primary culture medium special for panax notoginseng tissue culture seedlings |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993938A (en) * | 2016-05-16 | 2016-10-12 | 云南农业大学 | Method for inducing seedling emergence of in-vitro panax notoginseng dormant buds through gibberellin |
| CN106718891A (en) * | 2016-12-04 | 2017-05-31 | 淄博精诚专利信息咨询有限公司 | A kind of pseudo-ginseng asexual reproduction method of efficient stable |
| CN108617504A (en) * | 2017-03-26 | 2018-10-09 | 黄振忠 | A kind of special Initial culture base of candidum tissue culturing seedling and preparation method thereof |
| CN112616672A (en) * | 2020-12-31 | 2021-04-09 | 云南农业大学 | Method for directly inducing seedling emergence by utilizing stem segments of panax notoginseng |
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| CN1947498A (en) * | 2006-08-31 | 2007-04-18 | 上海交通大学 | Method for tissue-culture and induction of adventitous root of pseudo-ginseng |
| CN102210265A (en) * | 2010-04-02 | 2011-10-12 | 上海天龙药业有限公司 | Method for cultivating adventitious roots of pseudo-ginseng |
| CN103477991A (en) * | 2013-10-17 | 2014-01-01 | 黄振忠 | Primary culture medium specially used for tissue culture seedling culture of radix sophorae subprostratae |
| CN103503776A (en) * | 2013-10-12 | 2014-01-15 | 黄振忠 | Radix sophorae tonkinensis tissue culture seedling raising method |
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2015
- 2015-05-15 CN CN201510245183.8A patent/CN104823854A/en active Pending
Patent Citations (4)
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|---|---|---|---|---|
| CN1947498A (en) * | 2006-08-31 | 2007-04-18 | 上海交通大学 | Method for tissue-culture and induction of adventitous root of pseudo-ginseng |
| CN102210265A (en) * | 2010-04-02 | 2011-10-12 | 上海天龙药业有限公司 | Method for cultivating adventitious roots of pseudo-ginseng |
| CN103503776A (en) * | 2013-10-12 | 2014-01-15 | 黄振忠 | Radix sophorae tonkinensis tissue culture seedling raising method |
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Non-Patent Citations (2)
| Title |
|---|
| SHOYAMA,Y, ET AL.: "Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets", 《PLANT CELL REPORTS》, vol. 16, no. 7, 30 April 1997 (1997-04-30), pages 450 - 453 * |
| 张智慧等: "三七组织与细胞培养研究进展", 《云南农业大学学报》, vol. 19, no. 4, 31 August 2004 (2004-08-31), pages 369 - 372 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105993938A (en) * | 2016-05-16 | 2016-10-12 | 云南农业大学 | Method for inducing seedling emergence of in-vitro panax notoginseng dormant buds through gibberellin |
| CN105993938B (en) * | 2016-05-16 | 2018-01-02 | 云南农业大学 | A kind of method using the in vitro pseudo-ginseng resting bud emergence of gibberellin inducement |
| CN106718891A (en) * | 2016-12-04 | 2017-05-31 | 淄博精诚专利信息咨询有限公司 | A kind of pseudo-ginseng asexual reproduction method of efficient stable |
| CN108617504A (en) * | 2017-03-26 | 2018-10-09 | 黄振忠 | A kind of special Initial culture base of candidum tissue culturing seedling and preparation method thereof |
| CN112616672A (en) * | 2020-12-31 | 2021-04-09 | 云南农业大学 | Method for directly inducing seedling emergence by utilizing stem segments of panax notoginseng |
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Application publication date: 20150812 |
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