CN105954532A - Bio-cup contact type micro-array biochip sample application system - Google Patents
Bio-cup contact type micro-array biochip sample application system Download PDFInfo
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- CN105954532A CN105954532A CN201610472374.2A CN201610472374A CN105954532A CN 105954532 A CN105954532 A CN 105954532A CN 201610472374 A CN201610472374 A CN 201610472374A CN 105954532 A CN105954532 A CN 105954532A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
- G01N35/1074—Multiple transfer devices arranged in a two-dimensional array
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N2035/1027—General features of the devices
- G01N2035/1034—Transferring microquantities of liquid
- G01N2035/1037—Using surface tension, e.g. pins or wires
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Abstract
The invention discloses a Bio-cup contact type micro-array biochip sample application system. The system comprises a computer, a dust cover, a three-shaft robot, a sample application module, an ultrasonic cleaning and drying module and a control box, wherein the three-shaft robot, the sample application module and the ultrasonic cleaning and drying module are arranged in the dust cover; the computer and the control box are arranged outside the dust cover; the computer is connected with the three-shaft robot and is used for controlling the three-shaft robot to move; and the control box is connected with the sample application module and the ultrasonic cleaning and drying module and is used for controlling the sample application module and the ultrasonic cleaning and drying module to move. By virtue of a modular design and an integrated innovation mode, the Bio-cup contact type micro-array biochip sample application system is low in cost and high in automation degree and can meet public demands, the sample application cost of a biochip is lowered, the batch production is convenient, the research and development requirements of middle and small-sized enterprises and scientific research institutions as well as wide requirements of clinical diagnosis of hospitals are met, and a foundation is laid for the popularization and application of biochips.
Description
Technical field
The present invention relates to biochip point sample field of machines, particularly relate to a kind of Bio-cup contact microarray raw
Thing chip spotting system.
Background technology
Since biochip spotting robot (also known as point sample instrument) comes out, its technology achieves tremendous development,
By initially relatively easy laboratory self-control form to various powerful business point sample instruments, performance constantly changes
Kind, user interface is more friendly.At present, the some model machine that domestic corporation and research institution use is mostly state granddad
Department's product, owing to it is expensive, it is difficult to popularized at home.Most of pioneering enterprises, middle-size and small-size research
Mechanism's demand is urgent but unable buys by oneself, constrains innovation and the development of these enterprises to a certain extent.
Summary of the invention
It is an object of the invention to, it is provided that a kind of Bio-cup contact micro-array biochip spotting system.It
Using modularized design, integrated innovation is cheap, automaticity is high, meet public demand, reduces biology
The point sample cost of chip, it is simple to produce in batches to be applicable to medium-sized and small enterprises, scientific research institutions' research and development need and hospital
The vast demand of clinical diagnosis, lays the foundation for biochip popularization and application.
Technical scheme: a kind of Bio-cup contact micro-array biochip spotting system, including electricity
Brain, dust cover, three-axis robot, point sample module, ultrasonic cleaning irradiation modules and control chamber；
Described three-axis robot, point sample module and ultrasonic cleaning irradiation modules are all located at inside described dust cover；
Described computer and control chamber are located at outside described dust cover；
Described computer is connected with described three-axis robot, controls the motion of described three-axis robot；
Described control chamber is connected with described point sample module and described ultrasonic cleaning irradiation modules, controls described point sample
Module and the motion of described ultrasonic cleaning irradiation modules.
In aforesaid Bio-cup contact micro-array biochip spotting system,
Described point sample module includes that needle stand, described needle stand side are provided with cantilever, and described needle stand upper end has groove,
Described groove is inserted into spotting needle；
Described control chamber is installed by U-shaped base plate and U-shaped cover plate and is formed, and is provided with middle interval in the middle part of described control chamber
Plate, is divided into control chamber upper and lower two cavitys, is provided with membrane pump, vacuum pump and power supply, institute in described lower cavity
State power supply and be provided with ultrasonic module, in described upper plenum, be provided with relay and electrodeless speed regulator；
Described three-axis robot uses moving gantry type structure, and described three-axis robot includes mobile gantry, point sample
Module, X-axis module, Y-axis module, Z axis module, base plate, workbench and load sample module, described mobile dragon
Door is arranged in Y-axis module, and described Y-axis module is fixed on base plate, and described X-axis module is connected in described
In Y-axis module, described Z axis module is connected in described X-axis module, described Z axis module and described point sample
The cantilever of module connects, and described load sample module is placed on the work that the Y-axis module of described three-axis robot is provided with
On platform；
Described ultrasonic cleaning irradiation modules includes ultrasonic submodule, cleans submodule and vacuum drying submodule.
In aforesaid Bio-cup contact micro-array biochip spotting system, described load sample module includes load sample
Platform, on described load sample platform, the left side is provided with point sample district, and on described load sample platform, the right is sequentially provided with pre-point sample from front to back
District, sample stage, devil liquor recovery pond and ultrasonic pond, be provided with vacuum drying pond and clear in described devil liquor recovery pond
Wash pool.
In aforesaid Bio-cup contact micro-array biochip spotting system, described dust cover length × width × height sets
Being set to 70cm × 60cm × 60cm, described dust cover entirety is bottomless, is provided with and instead singly opens on front side of described dust cover
Door.
In aforesaid Bio-cup contact micro-array biochip spotting system, described three-axis robot length × wide ×
Height is set to 70cm × 50cm × 55cm.
In aforesaid Bio-cup contact micro-array biochip spotting system, described control chamber length × width × height sets
It is set to 30cm × 20cm × 20cm, in the middle of described U-shaped cover plate and described central dividing plate, is respectively equipped with two diameters 8
The circular open of cm, is used for placing 1000ml indigo plant mouth bottle, and this indigo plant mouth bottle cap is provided with diameter 0.1-2cm
Circular hole one, be used for inserting plastic tube, in blue mouth bottle, place sonification medium and devil liquor recovery respectively.
In aforesaid Bio-cup contact micro-array biochip spotting system, described ultrasonic submodule ultrasonic
Medium is ultra-pure water, PBST, ethanol or organic solvent, and ultrasonic power scope is 0-100W；Described cleaning
Module uses dual pathways membrane pump to control the turnover of cleanout fluid；Described vacuum drying submodule uses vacuum pump negative pressure
Suction dried；Described ultrasonic submodule, the liquid cleaned between submodule and vacuum drying submodule or gas
Employing plastic tube connects.
Compared with prior art, there is advantages that
A kind of Bio-cup contact micro-array biochip spotting system of the present invention uses modularized design, collection
One-tenth innovation is cheap, automaticity is high, meet public demand, reduces the point sample cost of biochip, just
In batch production with the vast need being applicable to medium-sized and small enterprises, scientific research institutions' research and development need and hospital clinical diagnoses
Ask, lay the foundation for biochip popularization and application.
Accompanying drawing explanation
Fig. 1 is the structural representation of three-axis robot of the present invention；
Fig. 2 is the structural representation of point sample module of the present invention；
Fig. 3 is the structural representation of dust cover of the present invention；
Fig. 4 is the structural representation of load sample module of the present invention；
Fig. 5 is the decomposition texture schematic diagram of control chamber of the present invention；
Fig. 6 is the structural representation of three-axis robot mobile gantry of the present invention；
Fig. 7 is the structural representation of three-axis robot Z axis module of the present invention；
Fig. 8 is the structural representation of three-axis robot X-axis module of the present invention；
Fig. 9 is the structural representation of three-axis robot Y-axis module of the present invention；
Figure 10 is the structural representation of three-axis robot workbench of the present invention；
Figure 11 is point sample scanning result of the present invention.
Being labeled as in accompanying drawing: 11-mobile gantry, 12-workbench, 13-X axle module, 14-Z axle module, 15-Y
Axle module, 16-point sample module, 17-base plate, 21-cantilever, 22-needle stand, 23-groove, 24-spotting needle, 31-
Dust cover, the upper anti-single open-door of 32-, 41-load sample platform, 42-point sample district, 43-pre-point sample district, 44-sample stage, 45-
Devil liquor recovery pond, 46-service sink, 47-is vacuum dried pond, the ultrasonic pond of 48-, 51-U type base plate, 52-U type lid
Plate, 53-central dividing plate, 54-membrane pump, 55-power supply, 56-ultrasonic module, 57-relay, 58-stepless time adjustment
Device, 59-circular open, 60-vacuum pump.
Detailed description of the invention
The present invention is further illustrated with embodiment below in conjunction with the accompanying drawings, but is not intended as limiting the present invention
Foundation.
Embodiment.A kind of Bio-cup contact micro-array biochip spotting system, is constituted as Figure 1-10 shows,
Including computer, dust cover, three-axis robot, point sample module, ultrasonic cleaning irradiation modules and control chamber；
Described three-axis robot, point sample module and ultrasonic cleaning irradiation modules are all located at inside described dust cover；
Described computer and control chamber are located at outside described dust cover；
Described computer is connected with described three-axis robot, controls the motion of described three-axis robot；
Described control chamber is connected with described point sample module and described ultrasonic cleaning irradiation modules, controls described point sample
Module and the motion of described ultrasonic cleaning irradiation modules.
Described point sample module includes needle stand 22, and described needle stand 22 side is provided with cantilever 21, on described needle stand 22
End has groove 23, and described groove 23 is inserted into spotting needle 24；
Described control chamber is installed by U-shaped base plate 51 and U-shaped cover plate 52 and is formed, and is provided with in the middle part of described control chamber
Central dividing plate 53, is divided into control chamber upper and lower two cavitys, is provided with membrane pump 54, vacuum in described lower cavity
Pump 60 and power supply 55, described power supply 55 is provided with ultrasonic module 56, is provided with relay 57 in described upper plenum
With electrodeless speed regulator 58；
Described three-axis robot uses moving gantry type structure, and described three-axis robot includes mobile gantry 11, point
Original mold block 16, X-axis module 13, Y-axis module 15, Z axis module 14, base plate 17, workbench 12 and load
Original mold block, described mobile gantry 11 is arranged in Y-axis module 15, and described Y-axis module 15 is fixed on base plate
On 17, described X-axis module 13 is connected in described Y-axis module 15, and described Z axis module 14 is connected in institute
Stating in X-axis module 13, described Z axis module 14 is connected with the cantilever 21 of described point sample module, described load sample
Module is placed on the workbench 12 that the Y-axis module 15 of described three-axis robot is provided with；
Described ultrasonic cleaning irradiation modules includes ultrasonic submodule, cleans submodule and vacuum drying submodule.
Described load sample module includes load sample platform 41, and on described load sample platform 41, the left side is provided with point sample district 42, described load
On sample platform 41 the right be sequentially provided with from front to back pre-point sample district 13, sample stage 44, devil liquor recovery pond 45 and
Ultrasonic pond 48, is provided with vacuum drying pond 47 and service sink 46 in described devil liquor recovery pond 45.
Described dust cover 31 length × width × height is set to 70cm × 60cm × 60cm, and described dust cover entirety is bottomless,
Anti-single open-door 32 it is provided with on front side of described dust cover.
Described three-axis robot length × width × height is set to 70cm × 50cm × 55cm.
Described control chamber length × width × height is set to 30cm × 20cm × 20cm, described U-shaped cover plate 52 and described in
It is respectively equipped with the circular open 59 of two diameter 8cm in the middle of space bar 53, is used for placing 1000ml indigo plant mouth bottle,
This indigo plant mouth bottle cap is provided with the circular hole one of diameter 0.1-2cm, is used for inserting plastic tube, in blue mouth bottle respectively
Place sonification medium and devil liquor recovery.
The sonification medium of described ultrasonic submodule is ultra-pure water, PBST, ethanol or organic solvent, ultrasonic power
Scope is 0-100W；Described cleaning submodule uses dual pathways membrane pump to control the turnover of cleanout fluid；Described very
The empty submodule that is dried uses vacuum pump negative pressure-pumping to be dried；Described ultrasonic submodule, cleaning submodule and vacuum
It is dried the liquid between submodule or gas uses plastic tube to connect.
In the Bio-cup contact micro-array biochip spotting system of the present invention, as it is shown on figure 3, dust-proof
Cover the 31 a height of 70cm × 60cm of length and width × 60cm.Dust cover 31 is overall bottomless, anti-single open-door 32 in employing
Design.Material therefor is plastics or metal, and thickness is 0.1mm to 2cm.Plastic material include PE, HDPE,
PP、PVC、GPPS、HIPS、ABS、PMMA、PET、PBT、PA、PC、POM、PPO、
PPS, PS etc., metal material includes ferrum, copper, aluminum, steel, titanium and alloy thereof.Upper anti-single open-door 31 size
For: 500mm × 250mm.
As shown in Fig. 1 and Fig. 6-Figure 10, three-axis robot length, width and height are set to 70cm × 50cm × 55cm.
Three-axis robot uses moving gantry type structure, and mobile gantry is arranged in Y-axis module.Three-axis robot uses
ADHON three-axis controller (model PMC-20MT-3), the motor 57HS5120A6 of Sumtor and essence
The type of drive of close ball-screw, controller uses engraving machine CNC.Legend computer is used to run Mach3 soft
Part carries out each unit operation (clean, be dried, sample, pre-point sample and point sample).Repetitive positioning accuracy design mesh
It is designated as 10 μm, maximal rate 0.3m/s, peak acceleration 10m/s2.Material be ferrum, copper, aluminum, magnesium, titanium,
Steel or the alloy of above-mentioned metal.Material thickness is 1mm to 3cm.
Performance parameter:
Horizontal X axle: range=300mm maximal rate V=10.0m/s peak acceleration a=20
m/s2。
Horizontal Y-axis: range=400mm maximal rate V=10.0m/s peak acceleration a=20
m/s2。
Vertical Z axle: range=100mm maximal rate V=10.0m/s peak acceleration a=10
m/s2。
As in figure 2 it is shown, point sample module is made up of cantilever, point sample needle stand and spotting needle.Wherein cantilever and spotting needle
The material of seat is metal, including ferrum, copper, aluminum, steel, titanium and alloy thereof.
Cantilever is fixedly linked by hexagon socket head cap screw with Z axis module, and needle stand is fixed by hexagon socket head cap screw with cantilever
It is connected.Spotting needle inserts point sample needle stand.Adjacent pin hole center distance is 9 ± 0.2mm.Spotting needle is crack pin,
Wide 0.1 ± the 0.01mm in crack.Spotting needle material is metal, including the rustless steel of all size, titanium, gold, each
Plant metal alloy etc..
As shown in Figure 4, load sample Module Division be ultrasonic, clean, be vacuum dried, be loaded, pre-point sample and point sample
In region.Work top size is for long × wide: 700mm × 390mm, thickness is 0-2cm.Work top material
Matter is plastics or metal.Plastic material include PE, HDPE, PP, PVC, GPPS, HIPS, ABS,
PMMA, PET, PBT, PA, PC, POM, PPO, PPS, PS etc., metal material include ferrum, copper,
Aluminum, steel, titanium and alloy thereof.Supersonic region is cylindrical, and a diameter of 7cm, the degree of depth is 0-10cm, ultrasonic
Box material is rustless steel, and thickness is 0-5mm.Cleaning area is uncovered cuboid, length × width × height: 128mm
×96mm×15mm；Vacuum drying region for there being lid cube, a height of 1.5cm × 1.5cm of length and width × 1.5cm,
Thickness is 2mm；The size of lid is 1.9cm × 1.9cm, and thickness is 0-10mm；Cube material used with lid
Matter is plastics, including PE, HDPE, PP, PVC, GPPS, HIPS, ABS, PMMA, PET,
PBT, PA, PC, POM, PPO, PPS, PS etc..Sample application zone is cuboid (96 orifice plate), size
For 12.8cm × 9.6cm × 0.5cm；Pre-point sample district is rectangle (microscope slide), and length × width × height is 2.5
cm×7.5cm×0.2cm；Point sample district is rectangle, length × a width of 22cm × 16cm.
Ultrasonic cleaning irradiation modules by ultrasonic, clean and three sub-module compositions such as vacuum drying.Wherein three sons
The working region of module is arranged on work top, and circuit control part is placed in control chamber, and control chamber is positioned at
Outside dust cover.The sonification medium of ultrasonic submodule is ultra-pure water, PBST, ethanol or organic solvent；Ultrasonic merit
Rate scope is 0-100W.Cleaning submodule uses dual pathways membrane pump to control the turnover of cleanout fluid；Vacuum drying
Submodule uses vacuum pump negative pressure-pumping to be dried.Liquid or the gas of each module are connected by plastic tube, plastic material
For PE, HDPE, PP, PVC, GPPS, HIPS, ABS, PMMA, PET, PBT, PA, PC,
POM, PPO, PPS, PS etc., manage a diameter of 0-2cm.
As it is shown in figure 5, control chamber length, width and height are set to 30cm × 20cm × 20cm.Control chamber is divided at the bottom of U-shaped
Plate, U-shaped cover plate and central dividing plate.Wherein there are two diameter 8cm respectively in the middle of U-shaped cover plate and central dividing plate
Circular open, be used for placing 1000ml indigo plant mouth bottle.Blue mouth bottle is respectively configured to provide cleanout fluid and collects waste liquid.
Bottom bottom right is placed with power supply and ultrasonic generator controller, places two membrane pumps, left inside side outside left side
One vacuum pump.On central dividing plate right side be placed with two electrodeless speed regulators, left-external side vertically place one wireless
The remote control time relay, one electronic time relay of left-external side horizontal positioned.Ultrasonic cleaning drying circuit sum
According to being connected to control chamber.
Experimental example is as follows:
1. material
1.1 reagent
Albumen sampling liquid: 40% glycerol, 0.01-0.04%SDS, 1 × PBS (pH 7.4)；
1 × the PBS or H of protein blocking liquid: 3%BSA2O；
Cleanout fluid: 1 × PBS+0.1%Tween20；
Anti-histidine-tagged fluorescent antibody: Penta His Alexa Fluor 532Conjugate (200 μ g/ml) uses
Albumen sampling liquid presses 1:100 dilution.
Anti-human fluorescent antibody working concentration: Alexa Fluor 546 (2mg/ml) albumen sampling liquid presses 1:1000
Dilution.
1.2 consumptive material
Microscope slide, poly-D-lysine microscope slide (safe).
1.3 instrument
Slide centrifuge (its woods Bel), laser confocal scanner (the rich Austria in Beijing).
2. operational approach
2.1 spotting needle pretreatment
Four crack pins are inserted in needle stand, after operation program, spotting needle successively in ultrasonic pond, service sink and
Vacuum drying stops 10 seconds in pond.
2.2 sampling
(1) the anti-histidine-tagged fluorescent antibody configured and anti-human fluorescent antibody are joined 96 holes of cleaning
In plate；Poly-D-lysine microscope slide substitutes common microscope slide, uses the Penta His Alexa Fluor after dilution
532；
(2) 96 orifice plates containing two kinds of fluorescent antibodys are positioned over sample application zone；
(3) running program, two kinds of antibody are sampled by crack pin simultaneously, and every kind of antibody is by arranging two pins
Sampling.
2.3 pre-point samples
Place a microscope slide clean, non-blooming in pre-point sample district, run program with point sample subsequently, will sampling
Fluorescent antibody point on microscope slide, point sample diameter is about 0.4mm.Ten points of every pin mark.
2.4 point sample
(1) 6 poly-D-lysine microscope slides clean, non-blooming are placed in point sample district；
(2) running point sample program, by the fluorescent antibody point of sampling on microscope slide, point sample diameter is about 0.4mm.
The spotting needle of every pin is classified as the subarray being set to 8 × 7, and in array, two point of sample center distance are set to 1
mm。
2.5 hatch
(1) microscope slide (protein chip) put is positioned over 37 DEG C of lucifuges and hatches 1h；
(2), after reaction, chip cleanout fluid cleans 5min, is repeated 3 times rear centrifuge dripping.
2.6 close and clean
(1) protein chip protein blocking liquid chamber temperature is closed 1h；
(2) after closing, chip cleanout fluid cleans 5min, is repeated 3 times rear centrifuge dripping.
2.7 scanning
(1), after enclosed cleaning, add ultra-pure water and be carried out 10min, centrifuge dripping；
(2), after cleaning, chip laser confocal scanner scans, preserves.
(3) point sample scanning result is shown in Figure 11.Substrate material is poly-D-lysine microscope slide, and in figure, left side is up and down
Four groups of submatrixs are classified as anti-human fluorescent antibody；In figure right side upper and lower four groups of submatrixs be classified as anti-histidine-tagged fluorescence resist
Body.
The above is the preferred embodiment of the present invention, certainly can not limit the right of the present invention with this
Scope, it is noted that for those skilled in the art, without departing from the principle of the invention
Under premise, it is also possible to make some improvement and variation, these improve and variation is also considered as protection scope of the present invention.
Claims (7)
1. a Bio-cup contact micro-array biochip spotting system, it is characterised in that include computer,
Dust cover, three-axis robot, point sample module, ultrasonic cleaning irradiation modules and control chamber；
Described three-axis robot, point sample module and ultrasonic cleaning irradiation modules are all located at inside described dust cover；
Described computer and control chamber are located at outside described dust cover；
Described computer is connected with described three-axis robot, controls the motion of described three-axis robot；
Described control chamber is connected with described point sample module and described ultrasonic cleaning irradiation modules, controls described point sample
Module and the motion of described ultrasonic cleaning irradiation modules.
Bio-cup contact micro-array biochip spotting system the most according to claim 1, its feature
It is,
Described point sample module includes that needle stand, described needle stand side are provided with cantilever, and described needle stand upper end has groove,
Described groove is inserted into spotting needle；
Described control chamber is installed by U-shaped base plate and U-shaped cover plate and is formed, and is provided with middle interval in the middle part of described control chamber
Plate, is divided into control chamber upper and lower two cavitys, is provided with membrane pump, vacuum pump and power supply, institute in described lower cavity
State power supply and be provided with ultrasonic module, in described upper plenum, be provided with relay and electrodeless speed regulator；
Described three-axis robot uses moving gantry type structure, and described three-axis robot includes mobile gantry, point sample
Module, X-axis module, Y-axis module, Z axis module, base plate, workbench and load sample module, described mobile dragon
Door is arranged in Y-axis module, and described Y-axis module is fixed on base plate, and described X-axis module is connected in described
In Y-axis module, described Z axis module is connected in described X-axis module, described Z axis module and described point sample
The cantilever of module connects, and described load sample module is placed on the work that the Y-axis module of described three-axis robot is provided with
On platform；
Described ultrasonic cleaning irradiation modules includes ultrasonic submodule, cleans submodule and vacuum drying submodule.
Bio-cup contact micro-array biochip spotting system the most according to claim 2, its feature
Being, described load sample module includes load sample platform, and on described load sample platform, the left side is provided with point sample district, on described load sample platform
The right is sequentially provided with pre-point sample district, sample stage, devil liquor recovery pond and ultrasonic pond from front to back, and described waste liquid returns
Vacuum drying pond and service sink it is provided with in receiving pond.
Bio-cup contact micro-array biochip spotting system the most according to claim 1, its feature
Being, described dust cover length × width × height is set to 70cm × 60cm × 60cm, and described dust cover entirety is bottomless,
Anti-single open-door it is provided with on front side of described dust cover.
Bio-cup contact micro-array biochip spotting system the most according to claim 1 and 2, its
Being characterised by, described three-axis robot length × width × height is set to 70cm × 50cm × 55cm.
Bio-cup contact micro-array biochip spotting system the most according to claim 2, its feature
Being, described control chamber length × width × height is set to 30cm × 20cm × 20cm, described U-shaped cover plate and described in
It is respectively equipped with the circular open of two diameter 8cm in the middle of space bar, is used for placing 1000ml indigo plant mouth bottle, this indigo plant
Mouth bottle cap is provided with the circular hole one of diameter 0.1-2cm, is used for inserting plastic tube, places respectively in blue mouth bottle
Sonification medium and devil liquor recovery.
Bio-cup contact micro-array biochip spotting system the most according to claim 2, its feature
Being, the sonification medium of described ultrasonic submodule is ultra-pure water, PBST, ethanol or organic solvent, ultrasonic merit
Rate scope is 0-100W；Described cleaning submodule uses dual pathways membrane pump to control the turnover of cleanout fluid；Described
Vacuum drying submodule uses vacuum pump negative pressure-pumping to be dried；Described ultrasonic submodule, clean submodule and true
The empty liquid being dried between submodule or gas use plastic tube to connect.
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| CN201610472374.2A CN105954532A (en) | 2016-06-23 | 2016-06-23 | Bio-cup contact type micro-array biochip sample application system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108398353A (en) * | 2018-06-07 | 2018-08-14 | 马鞍山钢铁股份有限公司 | A kind of ash sample transport mechanism of the carbon containing amount detecting device based on three-axis robot |
| CN113559947A (en) * | 2021-07-26 | 2021-10-29 | 杭州都林生物科技有限公司 | Automatic sample application instrument |
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| CN113559947B (en) * | 2021-07-26 | 2022-05-06 | 杭州都林生物科技有限公司 | Automatic sample application instrument |
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