CN101712034A - Cleaning device of integral sample application needles - Google Patents
Cleaning device of integral sample application needles Download PDFInfo
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- CN101712034A CN101712034A CN200910241631A CN200910241631A CN101712034A CN 101712034 A CN101712034 A CN 101712034A CN 200910241631 A CN200910241631 A CN 200910241631A CN 200910241631 A CN200910241631 A CN 200910241631A CN 101712034 A CN101712034 A CN 101712034A
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Abstract
The invention relates to sample application needle cleaning equipment field in a preparative technique of microarray chips, in particular relating to a cleaning device of integral sample application needles. The device comprises a support body which is processed with a through hole used for installing an ultrasonic rinsing compartment, a cleaning groove used for cleaning, and an air drying groove used for forming negative pressure in sequence. A support cover plate is installed on the support body; a through hole corresponding to sample application needle array is arranged at the matching part of the support cover plate and the air drying groove; the ultrasonic rinsing compartment is connected with an energy converter; the cleaning groove realizes water inflow and drainage through a water supply connector and a drainage connector which are arranged at the bottom; a clapboard is arranged between the water supply connector and the drainage connector; the cleaning groove is divided into a water storage pond and a drain water pond by the clapboard; and the air drying groove is connected with a vacuum pump through an air extractor head. The device has simple and compact structure, small occupied work area, short motion path of a sample application mechanical arm, improved washing efficiency, good cleaning effect and low cost, and can be applied to the sample application needle cleaning operation in the preparation process of the microarray chips.
Description
Technical field
The present invention relates to the spotting needle cleaning equipment field in the micro-array chip technology of preparing, relate in particular to a kind of all-in-one-piece spotting needle cleaning device.
Background technology
In the micro-array chip preparation process, spotting needle need dip in successively and get several samples, and some pull point finally forms microarray on substrate.For avoiding the cross pollution between the sample, spotting needle dip in get fresh sample before, need to clean and dip in the sample zone near the needle point.Therefore, the quality of cleaning and efficient have restricted the preparation quality and the preparation efficiency of micro-array chip to a great extent.
At present, most of micro-array chip preparation manipulations adopt the spotting needle cleaning method of syllogic, that is: at first, utilize " cavitation effect " that spotting needle is carried out ultrasonic cleaning, with the big molecular breakdown of remaining sample; Then, with distilled water or deionized water rinsing spotting needle; At last, air-dry or oven dry spotting needle.
Find by the investigation to existing patent: the spotting needle cleaning device in most of micro-array chip preparation systems is general to adopt existing separating component to form, and is about to miniature ultrasonic washer, small-sized flusher and small-sized air-dry apparatus and is installed in point sample manipulator working region simultaneously.This mode has the following disadvantages:
1, it is big to take point sample manipulator work area: clean requiredly with respect to spotting needle, existing separating component volume is excessive.One regularly in point sample manipulator working region, must need the corresponding substrate quantity of dwindling, thereby reduce the preparation efficiency of micro-array chip;
2, the spotting needle cleaning quality is poor: each separating component is not to clean this specific demand and design at spotting needle, therefore, can't make cleaning quality reach best;
3, point sample robot movement path length: in the spotting needle cleaning process, arrive each separating component position by point sample manipulator drive spotting needle and operate.For adapting to the installation site of each separating component, the point sample manipulator needs long motion path, thereby reduces cleaning efficiency.
Summary of the invention
The present invention provides a kind of all-in-one-piece spotting needle cleaning device for overcoming the existing technical deficiency of spotting needle cleaning equipment.
The present invention adopts following technical scheme:
On supporter, process through hole, the flushed channel that is used to wash that is used to install ultrasonic cleaning tank, the air-dry groove that is used to form negative pressure successively, the supporter cover plate is installed in the supporter top, has and the corresponding through hole of spotting needle array with air-dry groove mating part on the supporter cover plate; Described ultrasonic cleaning tank is installed in supporter and is used to install in the through hole of ultrasonic cleaning tank, and the ultrasonic cleaning tank lower end is connected with transducer, is used for causing ultrasonic cleaning tank cleaning liquid inside generation cavitation, reaches to decompose on the spotting needle to adhere to macromolecular purpose; Flushed channel is tank and blowoff basin two parts by the separator lined at its middle part, and the bottom of blowoff basin and tank is provided with drainage joint and water supply connector respectively; Drainage joint is connected with waste water container by pipeline, and water supply connector is connected with the water purification container by pipeline and peristaltic pump; The water that peristaltic pump drives in the water purification container enters flushed channel, cleans spotting needle, then enters waste water container; Air-dry trench bottom is provided with air extraction connector, and is connected with vavuum pump, and vavuum pump produces negative pressure in air-dry groove, and outside air is fast by slit between spotting needle and the through hole, dry spotting needle.
The lateral symmetry line of described ultrasonic cleaning tank, flushed channel, air-dry groove overlaps, and closely arranges, and makes spotting needle in cleaning process, and point sample manipulator operating path is the shortest.
The height of the dividing plate at described flushed channel middle part is lower than the degree of depth of flushed channel, thereby guarantees liquid level stabilizing in the tank.
The internal diameter of described water supply connector guarantees that less than the internal diameter of drainage joint water can not overflow flushed channel.
Via design with air-dry groove mating part on the described supporter cover plate is the manhole array, array arrangement is identical with the spotting needle array, Circularhole diameter is greater than near the needle body diameter spotting needle needle point, and this design can obtain maximum speed air flow, improves drying efficiency.
Beneficial effect of the present invention is: this apparatus structure is simply compact, and it is little to take the working region, and point sample robot movement path is short, has improved cleaning efficiency; Cleaning performance is good; Cost is low, can be widely used in the spotting needle cleaning operation in the micro-array chip preparation process.
Description of drawings
Fig. 1 is the all-in-one-piece spotting needle cleaning device structural representation of one embodiment of the present invention;
Fig. 2 is the vertical view of supporter;
Fig. 3 is the generalized section of A-A face among Fig. 2;
Fig. 4 is the vertical view of supporter cover plate;
Fig. 5 is the generalized section of B-B face among Fig. 4.
Number in the figure:
The 1-supporter; The 2-ultrasonic cleaning tank; 3-supporter cover plate; The 4-flushed channel; The air-dry groove of 5-; The 6-transducer; The 7-drainage joint; The 8-waste water container; 9-water purification container; The 10-peristaltic pump; The 11-water supply connector; The 12-vavuum pump; The 13-air extraction connector; The 14-tank; The 15-dividing plate; The 16-blowoff basin.
The specific embodiment
The present invention propose a kind of all-in-one-piece spotting needle cleaning device, below in conjunction with the description of drawings and the specific embodiment this explanation is described further.
The all-in-one-piece spotting needle cleaning device of a kind of present embodiment of the present invention such as Fig. 1-shown in Figure 5.Supporter 1 is the main components of whole device, processes through hole, the flushed channel 4 that is used to wash that is used to install ultrasonic cleaning tank 2, the air-dry groove 5 that is used to form negative pressure on supporter 1 successively.The lateral symmetry line of ultrasonic cleaning tank 2, flushed channel 4, air-dry groove 5 overlaps, and closely arranges, and makes spotting needle in cleaning process, and point sample manipulator operating path is the shortest.Supporter cover plate 3 is installed in supporter 1 top, have and the corresponding through hole of spotting needle array with air-dry groove 5 mating parts on the supporter cover plate 3, this through hole is the manhole array, and array arrangement is identical with the spotting needle array, and Circularhole diameter is greater than near the needle body diameter spotting needle needle point.Described ultrasonic cleaning tank 2 is installed in supporter 1 and is used to install in the through hole of ultrasonic cleaning tank 2, and ultrasonic cleaning tank 2 lower ends are connected with transducer 6, is used for causing ultrasonic cleaning tank 2 cleaning liquid inside generation cavitations, reaches to decompose on the spotting needle to adhere to macromolecular purpose.Flushed channel 4 is divided into the blowoff basin 16 in left side and tank 14 two parts on right side by the dividing plate 15 at its middle part, and the height of the dividing plate 15 at flushed channel 4 middle parts is lower than the degree of depth of flushed channel 4, thereby guarantees liquid level stabilizing in the tank.The bottom of blowoff basin 16 and tank 14 is provided with drainage joint 7 and water supply connector 11 respectively, drainage joint 7 is connected with waste water container 8 by pipeline, water supply connector 11 is connected with water purification container 9 by pipeline and peristaltic pump 10, the water that peristaltic pump 10 drives in the water purification container 9 enters flushed channel 4, clean spotting needle, then enter waste water container 8; The internal diameter of water supply connector 11 guarantees that less than the internal diameter of drainage joint 7 water can not overflow flushed channel.Air-dry groove 5 bottoms are provided with air extraction connector 13, and are connected with vavuum pump 12, and vavuum pump 12 produces negative pressure in air-dry groove 5, and outside air is fast by slit between spotting needle and the through hole, dry spotting needle.
In the spotting needle cleaning process, the point sample manipulator drives the spotting needle array and at first arrives ultrasonic cleaning tank 2 tops, transducer 6 is started working, the point sample manipulator moves downward, the tip portion that spotting needle is to be cleaned immerses below the ultrasonic cleaning tank 2 cleaning liquid inside liquid levels, by the cavitation effect that ultrasonic wave causes, will be attached to the large biological molecule fragmentation of spotting needle needle point.The point sample manipulator moves upward, and transducer 6 quits work.The point sample manipulator drives tank 14 tops of spotting needle array horizontal movement to flushed channel 4, peristaltic pump 10 is started working, distilled water or deionized water in the water purification container 9 are pumped in the tank 14 by water supply connector 11, unnecessary water is crossed dividing plate 15 and is entered blowoff basin 16, and enters waste water container 8 by drainage joint 7.The point sample manipulator moves downward, and the spotting needle tip portion is immersed below tank 14 liquid levels, and current will be attached to the large biological molecule flush of spotting needle needle point.The point sample manipulator moves upward, and peristaltic pump 10 quits work.The point sample manipulator drives the horizontal movement of spotting needle array to air-dry groove 5 tops, and makes the spotting needle array corresponding with the manhole array on the supporter cover plate 3, and vavuum pump 12 is started working.The point sample manipulator moves downward, and spotting needle enters air-dry groove 5 by manhole, and air fast by the slit between spotting needle and the manhole, makes distilled water or deionized water rapid evaporation attached to the spotting needle tip portion under the effect of vavuum pump 12.The point sample manipulator moves upward, and vavuum pump 12 quits work.Whole spotting needle cleaning process finishes.Because the lateral symmetry line of ultrasonic cleaning tank 2, flushed channel 4 and air-dry groove 5 overlaps, and closely arranges, so the horizontal movement of point sample manipulator only need carry out in one direction, and operating path is the shortest.
Above embodiment only is used to illustrate the present invention; and be not limitation of the present invention; row closes the those of ordinary skill of technical field; under the situation that does not break away from the spirit and scope of the present invention; can also make various variations and modification; therefore all technical schemes that are equal to also belong to category of the present invention, and scope of patent protection of the present invention should be defined by the claims.
Claims (5)
1. all-in-one-piece spotting needle cleaning device, it is characterized in that, processing is used to the air-dry groove (5) the through hole of ultrasonic cleaning tank (2), the flushed channel that is used to wash (4) being installed, being used to form negative pressure successively on supporter (1), supporter cover plate (3) is installed in supporter (1) top, and supporter cover plate (3) is gone up with air-dry groove (5) mating part and had and the corresponding through hole of spotting needle array; Described ultrasonic cleaning tank (2) is installed in supporter (1) and is used to install in the through hole of ultrasonic cleaning tank (2), ultrasonic cleaning tank (2) lower end is connected with transducer (6), be used for causing ultrasonic cleaning tank (2) cleaning liquid inside generation cavitation, reach to decompose on the spotting needle and adhere to macromolecular purpose; Flushed channel (4) is divided into blowoff basin (16) and tank (14) two parts by the dividing plate (15) at its middle part, and the bottom of blowoff basin (16) and tank (14) is provided with drainage joint (7) and water supply connector (11) respectively; Drainage joint (7) is connected with waste water container (8) by pipeline, and water supply connector (11) is connected with water purification container (9) by pipeline and peristaltic pump (10); The water that peristaltic pump (10) drives in the water purification container (9) enters flushed channel (4), cleans spotting needle, then enters waste water container (8); Air-dry groove (5) bottom is provided with air extraction connector (13), and is connected with vavuum pump (12), and vavuum pump (12) produces negative pressure in air-dry groove (5), and outside air is fast by slit between spotting needle and the through hole, dry spotting needle.
2. a kind of all-in-one-piece spotting needle cleaning device according to claim 1 is characterized in that, the lateral symmetry line of described ultrasonic cleaning tank (2), flushed channel (4), air-dry groove (5) overlaps.
3. a kind of all-in-one-piece spotting needle cleaning device according to claim 1 is characterized in that, the height of the dividing plate (15) at described flushed channel (4) middle part is lower than the degree of depth of flushed channel (4), thereby guarantees liquid level stabilizing in the tank.
4. a kind of all-in-one-piece spotting needle cleaning device according to claim 1 is characterized in that, the internal diameter of described water supply connector (11) guarantees that less than the internal diameter of drainage joint (7) water can not overflow flushed channel.
5. a kind of all-in-one-piece spotting needle cleaning device according to claim 1, it is characterized in that, described supporter cover plate (3) is gone up and the via design of air-dry groove (5) mating part is the manhole array, array arrangement is identical with the spotting needle array, and Circularhole diameter is greater than near the needle body diameter spotting needle needle point.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102416311A CN101712034B (en) | 2009-11-27 | 2009-11-27 | Cleaning device of integral sample application needles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102416311A CN101712034B (en) | 2009-11-27 | 2009-11-27 | Cleaning device of integral sample application needles |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101712034A true CN101712034A (en) | 2010-05-26 |
| CN101712034B CN101712034B (en) | 2011-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102416311A Expired - Fee Related CN101712034B (en) | 2009-11-27 | 2009-11-27 | Cleaning device of integral sample application needles |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103447268A (en) * | 2012-06-05 | 2013-12-18 | 北京普源精电科技有限公司 | Automatic sample feeder and needle bath thereof |
| CN103949436A (en) * | 2014-05-08 | 2014-07-30 | 深圳市新产业生物医学工程股份有限公司 | Ultrasonic cleaning mechanism and cleaning method for sampling pin |
| CN105170548A (en) * | 2015-08-28 | 2015-12-23 | 东莞市普华精密机械有限公司 | Cleaning equipment and cleaning method for test probe |
| CN105817434A (en) * | 2016-05-27 | 2016-08-03 | 中国科学院广州生物医药与健康研究院 | Automatic washing device and method for needles |
| CN105954532A (en) * | 2016-06-23 | 2016-09-21 | 湖北医药学院 | Bio-cup contact type micro-array biochip sample application system |
| CN107214129A (en) * | 2017-07-12 | 2017-09-29 | 重庆中元汇吉生物技术有限公司 | The swab and method of a kind of two-way cleaning sampling needle |
| CN110142262A (en) * | 2019-05-24 | 2019-08-20 | 中国科学院苏州生物医学工程技术研究所 | Liquid distributes needle cleaning device |
-
2009
- 2009-11-27 CN CN2009102416311A patent/CN101712034B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103447268A (en) * | 2012-06-05 | 2013-12-18 | 北京普源精电科技有限公司 | Automatic sample feeder and needle bath thereof |
| CN103949436A (en) * | 2014-05-08 | 2014-07-30 | 深圳市新产业生物医学工程股份有限公司 | Ultrasonic cleaning mechanism and cleaning method for sampling pin |
| CN103949436B (en) * | 2014-05-08 | 2016-09-21 | 深圳市新产业生物医学工程股份有限公司 | A kind of ultrasonic cleaning organization for sample needle and cleaning method |
| CN105170548A (en) * | 2015-08-28 | 2015-12-23 | 东莞市普华精密机械有限公司 | Cleaning equipment and cleaning method for test probe |
| CN105817434A (en) * | 2016-05-27 | 2016-08-03 | 中国科学院广州生物医药与健康研究院 | Automatic washing device and method for needles |
| CN105817434B (en) * | 2016-05-27 | 2018-05-22 | 中国科学院广州生物医药与健康研究院 | A kind of syringe needle automatic washing device and brushing method |
| CN105954532A (en) * | 2016-06-23 | 2016-09-21 | 湖北医药学院 | Bio-cup contact type micro-array biochip sample application system |
| CN107214129A (en) * | 2017-07-12 | 2017-09-29 | 重庆中元汇吉生物技术有限公司 | The swab and method of a kind of two-way cleaning sampling needle |
| CN107214129B (en) * | 2017-07-12 | 2023-01-31 | 重庆中元汇吉生物技术有限公司 | Swab and method for bidirectionally cleaning sampling needle |
| CN110142262A (en) * | 2019-05-24 | 2019-08-20 | 中国科学院苏州生物医学工程技术研究所 | Liquid distributes needle cleaning device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101712034B (en) | 2011-06-29 |
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Granted publication date: 20110629 Termination date: 20181127 |
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