CN105950512B - Microbial agent for removing COD in sewage and preparation method and application thereof - Google Patents
Microbial agent for removing COD in sewage and preparation method and application thereof Download PDFInfo
- Publication number
- CN105950512B CN105950512B CN201610389709.4A CN201610389709A CN105950512B CN 105950512 B CN105950512 B CN 105950512B CN 201610389709 A CN201610389709 A CN 201610389709A CN 105950512 B CN105950512 B CN 105950512B
- Authority
- CN
- China
- Prior art keywords
- culture medium
- culturing
- hours
- culture
- pichia pastoris
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
Abstract
The invention discloses a microbial agent for removing COD in sewage, a preparation method and an application thereof, and the microbial agent comprises Bacillus megaterium ACCC01032, Bacillus subtilis CICC23585, Paenibacillus sp CICC10358, Pichia pastoris ACCC21021 and Aspergillus flavus CGMCC 3.2823. The invention can effectively remove COD in the sewage in the biological treatment section, and the removal rate can reach about 95 percent; and has excellent impact resistance in the initial stage of system start-up or in the case of unstable operation. The product does not contain pathogenic bacteria, heavy metal and toxic chemical substances, and the solid product is nontoxic and non-irritant.
Description
Technical Field
The invention belongs to the technical field of biological sewage treatment in environmental protection engineering, and particularly relates to a microbial agent for removing COD (chemical oxygen demand) in sewage and application of the microbial agent in effectively removing ammonia nitrogen in sewage.
Background
The biological method of sewage treatment is widely applied to the treatment of industrial sewage, domestic sewage, rivers and lakes as an economic and effective water treatment mode, the biological method of sewage treatment is to remove pollutants in water by utilizing microbial metabolism and finally convert the pollutants into H 2 O, N 2, CO 2 and the like, and compared with a physical and chemical method, the biological method eliminates or reduces secondary pollution.
The microbial flora is used as the core of biological sewage treatment, and the technical key is as follows: and (3) screening, culturing and domesticating to obtain the flora capable of quickly and efficiently removing pollutants.
the synergistic strain in the microbial agent is obtained from a microbial strain preservation center. And activating by a specific culture medium, and compounding the strains according to different proportions to prepare the microbial agent for removing ammonia nitrogen. The sewage treatment system has long culture and domestication time by utilizing natural sludge, unstable treatment efficiency and poor impact resistance. The addition of the microbial agent can effectively shorten the domestication time, improve the effluent quality and the system stability, and comprehensively improve the pollutant removal capacity of the sewage treatment system; and for a system with poor running state or about to crash, the system can be quickly recovered to the normal running state by adding the microbial agent, and even the effluent water standard-lifting discharge can be achieved.
Disclosure of Invention
this section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
the present invention has been made in view of the above and/or other problems occurring in the conventional sewage treatment.
Therefore, one of the purposes of the present invention is to solve the deficiencies in the prior art and provide a microbial agent capable of effectively removing ammonia nitrogen in sewage.
In order to solve the technical problems, the invention provides the following technical scheme: a microbial agent for removing COD in sewage is calculated by the volume ratio of a bacterial liquid cultured by a single pure strain to the last logarithm, and comprises 25-35% of bacillus megaterium, 27-32% of bacillus subtilis, 30-32% of paenibacillus, 3-6% of pichia pastoris and 3-6% of aspergillus flavus.
As a preferable scheme of the microbial agent for removing COD in sewage, the invention comprises the following steps: the microbial agent for removing COD in sewage is calculated by the volume ratio (V/V) of bacterial liquid activated by single pure bacteria to the last logarithmic phase, and comprises 32% of bacillus megaterium, 32% of bacillus subtilis, 28% of paenibacillus, 5% of pichia pastoris and 3% of aspergillus flavus.
The invention also aims to provide a preparation method of the microbial agent capable of effectively removing ammonia nitrogen in sewage.
in order to solve the technical problems, the invention provides the following technical scheme: a preparation method of a microbial agent for removing COD in sewage comprises the steps of inoculating various strains into a liquid seed activation culture medium for activation, then inoculating the strains into an amplification culture medium for primary culture in an inoculation amount of 1-3%, then inoculating a bacterial liquid obtained by the primary culture into another amplification culture medium in an inoculation amount of 1-3% for secondary culture, and mixing and uniformly mixing bacterial liquids obtained by the secondary culture according to a proportion to prepare the microbial agent.
As a preferable scheme of the preparation method of the microbial agent for removing COD in sewage, the method comprises the following steps: the strains comprise one or more of bacillus megaterium, bacillus subtilis, paenibacillus, pichia pastoris or aspergillus flavus.
As a preferable scheme of the preparation method of the microbial agent for removing COD in sewage, the method comprises the following steps: the activation comprises culturing bacillus megatherium, bacillus subtilis and paenibacillus for 10-14 hours at 35-38 ℃; culturing the Pichia pastoris at 28-32 ℃ for 22-24 hours; culturing the aspergillus flavus at the temperature of 26-30 ℃ for 33-36 hours.
As a preferable scheme of the preparation method of the microbial agent for removing COD in sewage, the method comprises the following steps: the liquid seed activation culture medium comprises the components of activated bacillus megatherium, bacillus subtilis and paenibacillus by mass percent, wherein the components of the culture medium comprise 0.8-1.2% of peptone, 0.8-1.2% of sodium chloride, 0.4-0.6% of yeast extract and 0.5-1.5% of NaOH, the pH value of the culture medium is adjusted to 6.5-7.5, and the culture medium is sterilized at the temperature of 115 ℃ for 20 minutes; the culture medium for activating the pichia pastoris comprises, by mass, 0.8-1.2% of yeast extract, 1.5-2.5% of peptone and 1-3% of sucrose, and the culture medium is sterilized at 115 ℃ for 20 minutes; the culture medium for activating the aspergillus flavus comprises, by mass, 18-22% of potato leachate and 1.8-2.2% of glucose, and is sterilized for 20 minutes at 115 ℃.
As a preferable scheme of the preparation method of the microbial agent for removing COD in sewage, the method comprises the following steps: the first-stage culture comprises culturing the bacillus megaterium, the bacillus subtilis and the paenibacillus at 35-38 ℃ and 200-240 rpm for 10-14 hours; culturing the Pichia pastoris at 28-32 ℃ and 180-220 rpm for 16-20 hours; culturing the aspergillus flavus at 26-30 ℃ and 130-170 rpm for 33-36 hours.
As a preferable scheme of the preparation method of the microbial agent for removing COD in sewage, the method comprises the following steps: the secondary culture comprises culturing a bacterial liquid obtained by primary culture of bacillus megatherium, bacillus subtilis and paenibacillus for 9-11 hours at 35-38 ℃ and 200-240 rpm; culturing a bacterial liquid obtained by the primary culture of the pichia pastoris at the temperature of 28-32 ℃ and the rpm of 180-220 for 14-16 hours; culturing the bacterial liquid obtained by the first-stage culture of the aspergillus flavus at the temperature of 26-30 ℃ and the rpm of 130-170 for 23-25 hours.
The preferable scheme of the preparation method of the microbial agent for removing COD in sewage comprises that the amplification culture medium comprises, by mass, 0.8-1.2% of molasses, 0.4-0.6% of yeast extract and 0.8-1.2% of sodium chloride, the pH value is adjusted to 6.5-7.5, the culture medium is sterilized at 115 ℃ for 20 minutes, the amplification culture medium of Pichia pastoris comprises, by mass, 1.8-2.2% of molasses and 0.8-1.2% of yeast extract, the culture medium is sterilized at 115 ℃ for 20 minutes, the amplification culture medium of Aspergillus flavus comprises, by mass, 2.5-3.5% of molasses, 0.08-0.12% of K 2 HPO 4, 0.15-0.25% of NO and the culture medium is sterilized at 20 ℃ for 52 minutes.
The invention also provides application of the microbial agent capable of effectively removing ammonia nitrogen in sewage.
In order to solve the technical problems, the invention provides the following technical scheme: a method for removing COD in sewage by using a microbial agent comprises the steps of adding the microbial agent for 5 days every week in a cycle of four weeks, wherein the microbial agent for removing COD in the sewage is added on the 1 st day and the 2 nd day of the first week and the second week according to the volume ratio of the microbial agent to the sewage of 3-5: 1000; on the 3 rd day of the first week and the second week, adding a microbial agent for removing COD in the sewage according to the volume ratio of the microbial agent to the sewage of 2-4: 1000; adding a microbial agent for removing COD in the sewage on the 4 th day and the 5 th day of the first week and the second week according to the volume ratio of the microbial agent to the sewage of 1-2: 1000; and adding the microbial inoculum for removing COD in the sewage according to the volume ratio of the microbial inoculum to the sewage of 1-3: 1000 in the third week and the fourth day from 1 to 5.
the invention can effectively remove ammonia nitrogen in the sewage in a biological treatment section, and the removal rate can reach about 95 percent; and has excellent impact resistance in the initial stage of system start-up or in the case of unstable operation. The product does not contain pathogenic bacteria, heavy metal and toxic chemical substances, and the solid product is nontoxic and non-irritant. The practical application does not cause secondary pollution to the environment and additional water burden, and belongs to an environment-friendly compatible microbial agent product. And the product is simple, convenient and efficient to store, transport and put in.
Drawings
in order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:
FIG. 1 is a design diagram of SBR simulation process.
FIG. 2 is a process design diagram of the A/O simulation system.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
The invention firstly provides a microbial agent for effectively removing COD in sewage treatment. The microbial inoculum comprises Bacillus megaterium ACCC01032, Bacillus subtilis CICC23585, Bacillus Paenibucillus SP CICC10358, Pichia pastoris ACCC21021 and Aspergillus flavus CGMCC 3.2823. The invention sets the serial number of the microbial inoculum: COD-01.
The microbial inoculum COD-01 has the characteristics that: the strains and the proportion thereof are as follows: bacillus megaterium ACCC01032, 32%; bacillus subtilis CICC23585, 32%; paenibacillus sp CICC10358, 28%; pichia pastoris ACCC21021, 5%; aspergillus flavus CGMCC3.2823, 3%.
wherein, the proportion of each strain in the microbial inoculum refers to the volume ratio of the bacterial liquid cultured by a single pure strain to the last logarithmic phase. The microbial inoculum is prepared by the following steps:
(1) Taking out each strain preserved in the glycerol freezing tube from a refrigerator at the temperature of minus 70 ℃, thawing in an ultra-clean workbench sterilized by ultraviolet, and aseptically sucking 1ml by a pipette and inoculating into sterilized 10ml of liquid seed activation culture medium for activation.
(2) The liquid activation culture medium used in the step (1) of preparing the microbial inoculum comprises three types. The culture medium respectively comprises culture media of activated strains of bacillus megaterium ACCC01032, bacillus subtilis CICC23585 and paenibacillus sp CICC10358, and comprises the following components: 1% peptone; 1% of sodium chloride and 0.5% of yeast extract; the pH was adjusted to 7.0 with 1% NaOH and sterilized at 115 ℃ for 20 minutes. A culture medium for activating pichia pastoris ACCC21021, consisting of: 1% of yeast extract, 2% of peptone and 2% of glucose, and sterilizing at 115 ℃ for 20 minutes. The culture medium for activating Aspergillus flavus CGMCC3.2823 comprises the following components: 20% of potato extract and 2% of glucose, and sterilizing at 115 ℃ for 20 minutes.
(3) Inoculating liquid activation culture medium, and culturing Bacillus megaterium ACCC01032, Bacillus subtilis CICC23585, and Paenibacillus Paenibacilus SP CICC10358 at 37 deg.C for 12 hr; culturing Pichia pastoris ACCC21021 at 30 ℃ for 24 hours; aspergillus flavus strain CGMCC3.2823 was cultured at 28 ℃ for 36 hours.
(4) And (4) sucking 1mL of the activated liquid culture in the step (3) under an aseptic condition, inoculating the liquid culture into 50mL of liquid amplification culture medium, and culturing liquid seeds. Culturing Bacillus megaterium ACCC01032, Bacillus subtilis CICC23585, and Paenibacillus Paenibucilus. SP CICC10358 at 37 deg.C and 220rpm for about 12 hr; culturing Pichia pastoris ACCC21021 at 30 ℃ and 200rpm for about 18 hours; aspergillus flavus strain CGMCC3.2823 was cultured at 28 ℃ and 150rpm for 36 hours.
(5) The first-stage seed liquid prepared by the first-stage culture in the step (4) comprises three culture mediums for culturing Bacillus megaterium ACCC01032, Bacillus subtilis CICC23585 and Paenibacillus Paenibacilus. SP CICC 1035821937, wherein the culture mediums comprise 1% of molasses, 0.5% of yeast extract, 1% of sodium chloride and the pH is adjusted to 7.0, the culture medium for culturing Pichia pastoris ACCC21021 comprises 2% of molasses and 1% of yeast extract, and the culture medium for culturing Aspergillus flavus CGMCC3.2823 by using natural pH. comprises 3% of molasses, 0.1% of K 2 HPO 4 and 0.2% of NaNO 3.
(6) inoculating the seed solution cultured in the step (4) into the fresh amplification culture medium in the step (5) in an inoculation amount of 2%. Culturing bacillus megaterium ACCC01032, bacillus subtilis CICC23585 and paenibacillus Paenibacilus SP CICC 1035810 for hours according to the culture conditions in the step (4); culturing Pichia pastoris ACCC 2102115 hours; aspergillus flavus strain CGMCC 3.282324 hr.
(7) and (3) mixing the secondary seed solution prepared by secondary culture in the step (6) according to 32% of bacillus megatherium, 32% of bacillus subtilis, 28% of paenibacillus, 5% of pichia pastoris and 3% of aspergillus flavus to prepare a microbial mixed microbial inoculum, wherein the viable bacteria concentration of the microbial inoculum is more than 10 8 CFU/mL, the microbial mixed microbial inoculum can be stably stored for 6 months at room temperature, and the microbial mixed microbial inoculum is mixed according to the proportion, can exert the condition of the optimal effect (but not limited to the condition), can adapt to various biochemical treatment systems, and has the optimal application conditions of temperature of 20-45 ℃ and pH of 6-8.5.
The prepared microbial inoculum is added into a small-sized simulated SBR (sequencing batch reactor) and A/O (anaerobic/Oxic) sewage treatment system according to the adding mode of the following table.
The design parameters of the small SBR simulation device are as follows:
(1) Effective tank capacity: 20L;
(2) The COD of the inlet water is about 1500 mg/L; the ammonia nitrogen is about 60 mg/L;
(3) The sludge load rate is 0.5;
(4) MLSS about 2880 mg/L;
(5) The temperature is 20-40 ℃;
(6) The drainage ratio m is 2/5;
(7) Each period was 12 hours, aerated for 10 hours, and settled for 2 hours.
The SBR simulation process is shown in figure 1. The SBR system completes water inlet, aeration, precipitation and drainage stages in one device. Feeding air from 1, discharging air from 2, discharging from 3, fully mixing liquid by aeration, and adding efficient microbial agent at the beginning of aeration.
The A/O system is designed as follows:
(1) the design pool capacity of the water inlet pool is 20L, and the flow rate is 40L/d; pool a 10L, HRT 6 h; o pool 20L, HRT 12 h; 20L of sedimentation tank.
(2) The COD of the inlet water is about 1500 mg/L; the ammonia nitrogen is about 60 mg/L;
(3) MLSS was calculated for the A and O tanks as COD 1500 mg/L. Calculating to obtain O pool MLSS which is 6000 mg/L; pool A MLSS is 3000 mg/L.
(4) Sludge reflux ratio: the reflux speed is 2 times of the water inlet speed.
(5) The temperature is about 20-40 ℃.
(6) The DO of the pool A is about 0.2-0.5 mg/L; and DO in the O pool is 2-4 mg/L.
The A/O simulation system process is shown in FIG. 2. The system is divided into: the water inlet tank (A), the anoxic tank (B), the aerobic tank (C) and the sedimentation tank (D) are spatially divided. Compared with a typical A/O system, the mixed liquid reflux system is not arranged, so that the energy consumption is saved. Respectively putting anaerobic and aerobic high-efficiency microbial agents into the B pool and the C pool.
Example 1:
the invention is used for removing COD (chemical oxygen demand) of laboratory simulation industrial wastewater (the pH value is adjusted to be about 7.5; the main components comprise molasses, yeast extract, glucose, soluble starch, inorganic salt and the like). The microbial inoculum is added into the SBR process according to a preset period, and the system is operated under the condition of 35 ℃. For the SBR system to be collapsed (inlet water COD is about 1300mg/L, outlet water COD is about 300mg/L, and removal rate is about 76.9%), the system is operated according to preset parameters to maintain stability through adding the microbial inoculum for one month, and then under the condition that the inlet water COD is about 1300 mg/L. Through the addition of the microbial inoculum, the COD of the effluent is reduced to 49.5mg/L, and the treatment rate reaches 96 percent.
Example 2:
the invention is used for removing COD of laboratory simulation industrial wastewater (same as example 1). The microbial inoculum is added to the A/O process according to a predetermined period, and the system is operated at 35 ℃. For the A/O system which is not well operated (the COD of inlet water is about 1300mg/L, the COD of outlet water is about 250mg/L, and the removal rate is about 80.7%), the system is operated according to the preset parameters, and after the system is stably maintained through adding the microbial inoculum for one month, under the condition that the COD of inlet water is about 1300 mg/L. By adding the microbial inoculum, the effluent is reduced to about 35mg/L, and the treatment rate reaches 97.2 percent.
Example 3:
The method is adopted to remove COD (chemical oxygen demand) of printing and dyeing wastewater (the pH value is preset to be 6.5-8.5; the main components comprise dye, surfactant and polyvinyl alcohol, and a proper amount of molasses, yeast extract, glucose and inorganic salt are added to meet the requirement of necessary nutrients of microorganisms) of textile and clothing colleges of south Jiangnan university. The microbial inoculum is added to the A/O process according to a predetermined period, and the system is operated at 35 ℃. For the A/O system which is not well operated (the inlet COD is about 1400mg/L, the outlet COD is about 500mg/L and the removal rate is about 64.3%), the system is operated according to the preset parameters, and after the system is stably maintained through the addition of the microbial inoculum for one month, under the condition that the inlet COD is about 1400 mg/L. By adding the microbial inoculum, the effluent is reduced to about 91mg/L, and the treatment rate reaches about 93.5 percent.
Example 4:
the COD of the printing and dyeing wastewater of the textile and clothing college of the south of the Yangtze river university (same as the example 3) is removed by adopting the method. The microbial inoculum is added into the SBR process according to a preset period, and the system is operated under the condition of 35 ℃. For the SBR system to be collapsed (the inlet water COD is about 1400mg/L, the outlet water COD is about 500mg/L, the removal rate is about 64.3%), adding the microbial inoculum for one month, operating the system according to the preset parameters to maintain the stability, and then under the condition that the inlet water COD is about 1400 mg/L. Through the addition of the microbial inoculum, the COD of the effluent is reduced to be below 100mg/L, and the treatment rate exceeds 92.8 percent.
Example 5: the effect of the microbial inoculum under other strain proportions on removing COD in the application is as follows:
The COD of the printing and dyeing wastewater of the textile and clothing college of the south of the Yangtze river university (same as the example 3) is removed by adopting the method. The proportion of the microbial inoculum is changed into 30 percent of bacillus megaterium ACCC 01032; bacillus subtilis CICC23585, 20%; paenibacillus sp CICC10358, 30%; pichia pastoris ACCC21021, 15%; aspergillus flavus CGMCC3.2823, 5%. The addition to the SBR process was carried out at a predetermined period and the system was run at 35 ℃. For the SBR system to be collapsed (the inlet water COD is about 1400mg/L, the outlet water COD is about 500mg/L, the removal rate is about 64.3%), adding the microbial inoculum for one month, operating the system according to the preset parameters to maintain the stability, and then under the condition that the inlet water COD is about 1400 mg/L. By adding the microbial inoculum, the COD of the effluent is maintained at about 180mg/L, and the treatment rate is about 87.1 percent.
example 6:
the COD of the printing and dyeing wastewater of the textile and clothing college of the south of the Yangtze river university (same as the example 3) is removed by adopting the method. The proportion of the microbial inoculum is changed into 20 percent of bacillus megaterium ACCC 01032; bacillus subtilis CICC23585, 20%; paenibacillus sp CICC10358, 30%; pichia pastoris ACCC21021, 20%; aspergillus flavus CGMCC3.2823, 10%. Added to the a/O process at a predetermined period and the system was run at 35 ℃. For an A/O system to be collapsed (the COD of the inlet water is about 1400mg/L, the COD of the outlet water is about 500mg/L, and the removal rate is about 64.3%), adding a microbial inoculum for one month, operating the system according to preset parameters to maintain stability, and then under the condition that the COD of the inlet water is about 1400 mg/L. By adding the microbial inoculum, the COD of the effluent is maintained below about 230mg/L, and the treatment rate exceeds 83.6 percent.
Therefore, the microorganism synergistic microbial inoculum for removing COD mainly comprises the following bacteria: bacillus megaterium ACCC01032, Bacillus subtilis CICC23585, Paenibacillus sp CICC10358, Pichia pastoris ACCC21021 and Aspergillus flavus CGMCC 3.2823. The microbial inoculum is in the form of microbial suspension, the preparation method comprises the steps of preserving, activating and expanding culture of various strains to obtain the bacterial suspension in the optimal growth period, and mixing the bacterial suspensions in proportion to obtain the liquid microbial inoculum.
the invention can effectively remove COD in the sewage in the biological treatment section, and the removal rate can reach about 95 percent; and has excellent impact resistance in the initial stage of system start-up or in the case of unstable operation. The product does not contain pathogenic bacteria, heavy metal and toxic chemical substances, and the solid product is nontoxic and non-irritant. The practical application does not cause secondary pollution to the environment and additional water burden, and belongs to an environment-friendly compatible microbial agent product. And the product is simple, convenient and efficient to store, transport and put in.
The foregoing description has disclosed fully embodiments of the invention. It should be noted that those skilled in the art can make modifications to the embodiments of the present invention without departing from the scope of the appended claims. Accordingly, the scope of the appended claims is not to be limited to the specific embodiments described above.
Claims (2)
1. A microbial agent for removing COD in sewage is characterized in that the microbial agent comprises Bacilusmegaterium ACCC 01032% of bacillus megaterium, Bacilussubtilis CICC 23585% of bacillus subtilis, Paenibacilus. SPCICC 10358% of paenibacillus, Pichia pastoris ACCC 21021% of pichia pastoris and Aspergilus flavus CGMCC 3.2823% of aspergillus flavus in a ratio of the volume of a bacterial liquid cultured by a single pure strain to the last logarithm stage;
Inoculating each strain into a liquid seed activation culture medium for activation, then inoculating the strain into an amplification culture medium by an inoculation amount of 1-3% for primary culture, then inoculating a bacterial liquid obtained by the primary culture into another amplification culture medium by an inoculation amount of 1-3% for secondary culture, and mixing and uniformly mixing the bacterial liquid obtained by the secondary culture according to a proportion to prepare a microbial agent;
the amplification culture medium comprises, by mass, 0.8-1.2% of molasses, 0.4-0.6% of yeast extract and 0.8-1.2% of sodium chloride, the pH value of the amplification culture medium is adjusted to 6.5-7.5, the culture medium is sterilized at 115 ℃ for 20 minutes, the amplification culture medium of pichia pastoris comprises, by mass, 1.8-2.2% of molasses and 0.8-1.2% of yeast extract, the culture medium is sterilized at 115 ℃ for 20 minutes, the amplification culture medium of aspergillus flavus comprises, by mass, 2.5-3.5% of molasses, 0.08-0.12% of K 2 HPO 4 and 0.15-0.25% of NaNO 3, and the culture medium is sterilized at 115 ℃ for 20 minutes.
2. the method for preparing a microbial agent for removing COD in sewage according to claim 1, wherein: the activation comprises culturing bacillus megatherium, bacillus subtilis and paenibacillus for 10-14 hours at 35-38 ℃; culturing the Pichia pastoris at 28-32 ℃ for 22-24 hours; culturing aspergillus flavus at 26-30 ℃ for 33-36 hours;
The liquid seed activation culture medium comprises the components of activated bacillus megatherium, bacillus subtilis and paenibacillus by mass percent, wherein the components of the culture medium comprise 0.8-1.2% of peptone, 0.8-1.2% of sodium chloride, 0.4-0.6% of yeast extract and 0.5-1.5% of NaOH, the pH value of the culture medium is adjusted to 6.5-7.5, and the culture medium is sterilized at the temperature of 115 ℃ for 20 minutes; the culture medium for activating the pichia pastoris comprises, by mass, 0.8-1.2% of yeast extract, 1.5-2.5% of peptone and 1-3% of sucrose, and the culture medium is sterilized at 115 ℃ for 20 minutes; the culture medium for activating the aspergillus flavus comprises, by mass, 18-22% of potato leachate and 1.8-2.2% of glucose, and the culture medium is sterilized for 20 minutes at the temperature of 115 ℃;
The first-stage culture comprises culturing the bacillus megaterium, the bacillus subtilis and the paenibacillus at 35-38 ℃ and 200-240 rpm for 10-14 hours; culturing the Pichia pastoris at 28-32 ℃ and 180-220 rpm for 16-20 hours; culturing aspergillus flavus at 26-30 ℃ and 130-170 rpm for 33-36 hours;
The secondary culture comprises culturing a bacterial liquid obtained by primary culture of bacillus megatherium, bacillus subtilis and paenibacillus for 9-11 hours at 35-38 ℃ and 200-240 rpm; culturing a bacterial liquid obtained by the primary culture of the pichia pastoris at the temperature of 28-32 ℃ and the rpm of 180-220 for 14-16 hours; culturing the bacterial liquid obtained by the first-stage culture of the aspergillus flavus at the temperature of 26-30 ℃ and the rpm of 130-170 for 23-25 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610389709.4A CN105950512B (en) | 2016-06-03 | 2016-06-03 | Microbial agent for removing COD in sewage and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610389709.4A CN105950512B (en) | 2016-06-03 | 2016-06-03 | Microbial agent for removing COD in sewage and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105950512A CN105950512A (en) | 2016-09-21 |
| CN105950512B true CN105950512B (en) | 2019-12-10 |
Family
ID=56907686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610389709.4A Active CN105950512B (en) | 2016-06-03 | 2016-06-03 | Microbial agent for removing COD in sewage and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105950512B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106745805B (en) * | 2016-12-20 | 2020-03-27 | 福建科宏生物工程股份有限公司 | Method for treating sucralose mother liquor wastewater after biodegradation pretreatment |
| CN106987535A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | It is a kind of for complex micro organism fungicide of river regulation and preparation method thereof, river sewage processing method |
| CN106987536A (en) * | 2017-03-01 | 2017-07-28 | 沃邦环保有限公司 | Complex micro organism fungicide for river regulation and its preparation method and application |
| CN107699513B (en) * | 2017-09-13 | 2020-08-21 | 博元生态修复（北京）有限公司 | Black and odorous water body degrading bacterium and application thereof |
| CN109370957B (en) * | 2018-12-10 | 2021-03-02 | 江南大学 | Sewage treatment composite microbial inoculum and preparation method thereof |
| CN109554315A (en) * | 2018-12-27 | 2019-04-02 | 黄河三角洲京博化工研究院有限公司 | A kind of black and odorous water microorganism remediation composite bacteria agent and its application |
| CN109851066A (en) * | 2019-04-01 | 2019-06-07 | 陕西科技大学 | A kind of biological reinforced microbial inoculum and its preparation method and application |
| CN110358705A (en) * | 2019-07-03 | 2019-10-22 | 东莞市顶盛环保科技有限公司 | A kind of removal trade effluent COD bacteria agent and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538538A (en) * | 2008-03-18 | 2009-09-23 | 上海环伟生物科技有限公司 | Composite microorganism viable bacteria preparation and preparation method and application of same |
| CN101973795A (en) * | 2010-10-21 | 2011-02-16 | 哈尔滨工业大学 | Sludge aerobic composting method by utilizing composting compound microbial inoculum |
-
2016
- 2016-06-03 CN CN201610389709.4A patent/CN105950512B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538538A (en) * | 2008-03-18 | 2009-09-23 | 上海环伟生物科技有限公司 | Composite microorganism viable bacteria preparation and preparation method and application of same |
| CN101973795A (en) * | 2010-10-21 | 2011-02-16 | 哈尔滨工业大学 | Sludge aerobic composting method by utilizing composting compound microbial inoculum |
Non-Patent Citations (1)
| Title |
|---|
| 不同种类微生物利用制酒废水生产微生物絮凝剂及其性能比较;张帆;《四川大学学报（工程科学版）》;20080920;第40卷(第5期);94-98 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105950512A (en) | 2016-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105950512B (en) | Microbial agent for removing COD in sewage and preparation method and application thereof | |
| CN106746159A (en) | A kind of processing method of antibiotic production wastewater | |
| CN104278002A (en) | Compound microbial agent for urban sewage treatment, preparation method and application thereof | |
| CN106635861A (en) | Salt-tolerant COD removal denitrifying microbial agent and preparation method thereof | |
| CN100431982C (en) | Method of inhibiting membrane contamination of membrane bioreactor | |
| CN102241459B (en) | Method for reinforcing adsorption-biodegradation (AB) denitrification technology by use of heterotrophic nitrification-aerobic denitrification bacteria | |
| CN107236687B (en) | Pseudomonas stutzeri with hexavalent chromium removal and aerobic denitrification performance and application thereof | |
| CN102701442A (en) | Quick start method of source water biological denitrification system | |
| CN115353198B (en) | Synchronous denitrification and dephosphorization method based on sponge iron-sodium thiosulfate autotrophic denitrification biological filter | |
| CN105217911A (en) | A kind of bioleaching that utilizes reacts the technological process control carrying out sludge dewatering | |
| CN106746161A (en) | A kind of rifamycin produces the processing method of waste water | |
| CN102220240A (en) | PM-I sludge reduction microbial agent | |
| CN102465102B (en) | Nitrous acid type biological denitrogenation microbial agent and application thereof | |
| CN105838653B (en) | A kind of microbial bacterial agent and the preparation method and application thereof | |
| CN217947785U (en) | Low C/N ratio waste water enhanced denitrification treatment system | |
| CN106119150A (en) | A kind of dephosphorization microbial bacterial agent and its preparation method and application | |
| CN102643748A (en) | Sludge-reduced microorganism combined dominant population in sewage processing | |
| CN114262679B (en) | Autotrophic denitrifying bacteria and rapid expanding culture process thereof | |
| CN105176902A (en) | Microbial preparation for urban sewage treatment and application thereof | |
| CN113292159B (en) | Enhanced CFBR (circulating fluidized bed reactor) process applied to high ammonia nitrogen-containing wig wastewater treatment | |
| CN113735392A (en) | Method for treating preserved fruit processing wastewater | |
| CN112591889A (en) | Denitrogenation method for microbial film reinforced film biological reactor | |
| CN113173650A (en) | Method for treating septic tank sewage by coupling compound microbial agent and calcium peroxide | |
| CN112175879A (en) | Culture method and application of synchronous heterotrophic nitrification-aerobic denitrification pseudomonas mendocina | |
| CN111547847A (en) | Device and method for removing total nitrogen in sewage by composite microorganism enhanced A/O (anaerobic/aerobic) process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |