CN105942529A - Method for preparing yeast extract with high I+G content - Google Patents
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- CN105942529A CN105942529A CN201610268471.XA CN201610268471A CN105942529A CN 105942529 A CN105942529 A CN 105942529A CN 201610268471 A CN201610268471 A CN 201610268471A CN 105942529 A CN105942529 A CN 105942529A
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- 229940041514 candida albicans extract Drugs 0.000 title claims abstract description 26
- 239000012138 yeast extract Substances 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 21
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 76
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 102000004190 Enzymes Human genes 0.000 claims abstract description 19
- 108090000790 Enzymes Proteins 0.000 claims abstract description 19
- 238000002360 preparation method Methods 0.000 claims abstract description 19
- 235000013336 milk Nutrition 0.000 claims abstract description 13
- 239000008267 milk Substances 0.000 claims abstract description 13
- 210000004080 milk Anatomy 0.000 claims abstract description 13
- 239000000203 mixture Substances 0.000 claims description 39
- 239000004365 Protease Substances 0.000 claims description 22
- 238000006243 chemical reaction Methods 0.000 claims description 19
- 229940088598 enzyme Drugs 0.000 claims description 18
- 108091005804 Peptidases Proteins 0.000 claims description 16
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 16
- 235000019419 proteases Nutrition 0.000 claims description 16
- 239000000047 product Substances 0.000 claims description 15
- 108090000340 Transaminases Proteins 0.000 claims description 14
- 102000003929 Transaminases Human genes 0.000 claims description 14
- 239000002671 adjuvant Substances 0.000 claims description 14
- 210000000481 breast Anatomy 0.000 claims description 14
- 238000001694 spray drying Methods 0.000 claims description 14
- 239000000284 extract Substances 0.000 claims description 13
- 239000012265 solid product Substances 0.000 claims description 13
- 101710163270 Nuclease Proteins 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical group O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 7
- 229930006000 Sucrose Natural products 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- 238000001976 enzyme digestion Methods 0.000 claims description 4
- 108010004032 Bromelains Proteins 0.000 claims description 2
- 108090000270 Ficain Proteins 0.000 claims description 2
- 108090000526 Papain Proteins 0.000 claims description 2
- 235000019835 bromelain Nutrition 0.000 claims description 2
- 235000019836 ficin Nutrition 0.000 claims description 2
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 claims description 2
- 235000019834 papain Nutrition 0.000 claims description 2
- 229940055729 papain Drugs 0.000 claims description 2
- 235000009392 Vitis Nutrition 0.000 claims 1
- 241000219095 Vitis Species 0.000 claims 1
- 238000001035 drying Methods 0.000 abstract description 6
- 238000000108 ultra-filtration Methods 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 2
- 230000002358 autolytic effect Effects 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- 230000009849 deactivation Effects 0.000 abstract 1
- 239000000446 fuel Substances 0.000 abstract 1
- 230000029219 regulation of pH Effects 0.000 abstract 1
- 238000010792 warming Methods 0.000 description 10
- 238000012545 processing Methods 0.000 description 7
- 239000002028 Biomass Substances 0.000 description 5
- 241000195493 Cryptophyta Species 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 238000009413 insulation Methods 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108020004707 nucleic acids Proteins 0.000 description 4
- 150000007523 nucleic acids Chemical class 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for preparing a yeast extract with high I+G content; the method comprises the following steps of yeast milk preparation, autolytic enzymolysis, enzyme deactivation and separation, ultrafiltration treatment, homogeneous concentration, and atomizing drying. Yeast milk with the protein content of more than 60% is used as the raw material; autolytic enzymolysis treatment is performed through strict temperature control and pH regulation; a certain amount of auxiliary RNA is added in a fuel; and finally, a clear liquid obtained from separation is subjected to ultrafiltration concentration treatment to obtain the yeast extract with the I+G content reaching 19.5% or more. According to the preparation method provided by the invention, the yeast extract with the I+G content reaching 19.5% can be prepared on a large scale.
Description
Technical field
The present invention relates to yeast extract preparing technical field, be specifically related to the system of a kind of high I+G content yeast extract
Preparation Method.
Background technology
Along with the expansion year by year of yeast extract application, it is widely used in infant and food for the aged, nutrition
The industry such as hardening agent, health product, although China is yeast extract big producing country of the world, but its I+ of the product generally prepared
G content is relatively low, and owing to I+G content more high value is the biggest, and the main cause affecting I+G content is protein in yeast
Content, in order to strengthen its nutrition and mouthfeel further, extract produce in use high protein, high nucleic acid content yeast former
Material, prepares the yeast extract that I+G content is higher, to meet demand to Seasoning Ingredients after people's living standard improves.
The present inventor by substantial amounts of experiment and research, for people provide a kind of I+G content can reach 19.5% with
On the preparation method of high I+G content yeast extract, the problem relatively low to solve existing yeast extract I+G content.
Summary of the invention
For solving above-mentioned technical problem, the invention provides the preparation method of a kind of high I+G content yeast extract.
The present invention is achieved by the following technical programs:
A kind of preparation method of high I+G content yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain to turn out high-protein yeast breast, the high-protein yeast breast that will obtain
PH controls as 6-8；
B, self-dissolving enzymolysis: yeast milk separating treatment obtains yeast mixture, yeast mixture enters self-dissolving tank, adds in yeast mixture
Auxiliary RNA makes the content of RNA in yeast mixture reach the 30-40% of total amount, adds adjuvant, protease, nuclease, transaminase,
Control pH and carry out self-dissolving enzyme digestion reaction；
C, enzyme denaturing separate: temperature is raised enzyme denaturing and is then peeled off；
D, hyperfiltration treatment: the clear liquid of isolated is carried out hyperfiltration treatment；
E, homogenizing concentrate: put in homogenizer by the clear liquid through hyperfiltration treatment, and the clear liquid completed by homogenizing is condensed into half
Solid product；
F, spray drying: semi-solid products is sent into spray drying tower internal spraying and is dried prepared extract finished product.
The concentration of the auxiliary RNA in described step b is 53-63%.
Adjuvant in described step b is white sugar and glucose forms according to the proportions that mass ratio is 1:1.
In described step b the addition of protease be the 0.1-0.7% of total amount, the 0.5-that addition is total amount of nuclease
1.0%, the addition of transaminase is the 0.05-0.15% of total amount.
One during protease is papain, ficin, bromelain in described step b.
In described step b, the self-dissolving enzymolysis response time is 16-20h.
In described step b, the pH value of self-dissolving enzyme digestion reaction controls as 4.5-6.8.
The beneficial effects of the present invention is: the present invention utilizes existing Wine brewing yeast strain to prepare protein content can
Reach the yeast milk of more than 60%, then with the yeast milk of high protein content as raw material, by strict temperature control, pH regulator carry out from
Molten enzymolysis processing, has carried out assisting the interpolation of RNA so that in yeast mixture, rna content reaches more than 30% during processing,
Lay a good foundation for preparing the yeast extract of high I+G content, finally the clear liquid of isolated is processed through ultrafiltration concentration
Prepare I+G content and reached the yeast extract of more than 19.5%, met the demand of people, improve economic worth.
Accompanying drawing explanation
Fig. 1 is the process chart of the present invention
Detailed description of the invention
Below in conjunction with drawings and Examples, technical scheme is further described, but claimed scope is also
It is not limited to described.
Embodiment one
A kind of preparation method of high I+G content yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain turn out protein content be 60%, rna content be 8.6%, sea
The high-protein yeast breast of algae sugar content≤4%, enters the high-protein yeast breast obtained tank and is heated to 75 DEG C and boils 85min, control pH
It is 6.1, is then cooled to 55 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters self-dissolving tank, to yeast mixture
Middle addition concentration is 53%, purity be 80% auxiliary RNA make the content of RNA in yeast mixture reach the 36% of total amount, add
Quality is the adjuvant of yeast mixture quality 4%, and adjuvant is formed according to the proportions that mass ratio is 1:1 by white sugar and glucose,
Controlling pH is 5.5, after stirring 10min, adds protease and controls after pH is 5.5 reaction 4h, then adds nucleic acid after being warming up to 58 DEG C
It is 5.9 reaction 10h that enzyme controls pH, then add transaminase to control pH to be 5.7 reaction 7h after being cooled to 41 DEG C, then temperature rises to 61 DEG C
Rear insulation 20min.The addition of protease is the 0.3% of total amount, the addition of nuclease is the 0.7% of total amount, transaminase
Addition is the 0.15% of total amount；
C, enzyme denaturing separate: the yeast mixture that self-dissolving enzymolysis completes is warming up to 85 DEG C and carries out enzyme denaturing process, and the process time is
30min, carries out centrifuging treatment after it cools down, and separating treatment rotating speed is 4000r/min, obtains residue after processing 30min
And clear liquid, residue is used for feedstuff and prepares；
D, hyperfiltration treatment: by the clear liquid temperature control of isolated to 50 DEG C, carry out hyperfiltration treatment under the pressure of 0.3MPa；
E, homogenizing concentrate: the clear liquid through hyperfiltration treatment is put in homogenizer homogenizing under the pressure of 5MPa, by homogenizing
The clear liquid temperature control completed makes, to 55 DEG C of concentrations, the semi-solid products that dry biomass mark is 37%；
F, spray drying: semi-solid products is sent into spray drying tower internal spraying and is dried prepared extract finished product, spray dried
Dry inlet temperature is 158 DEG C, and leaving air temp is 93 DEG C, and drying pressure is 13MPa, water content≤5% of extract finished product.
Embodiment two
A kind of preparation method of high I+G content yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain turn out protein content be 61%, rna content be 8.7%, sea
The high-protein yeast breast of algae sugar content≤4%, enters the high-protein yeast breast obtained tank and is heated to 93 DEG C and boils 55min, control pH
It is 6.7, is then cooled to 58 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters self-dissolving tank, to yeast mixture
Middle addition concentration is 53%, purity be 80% auxiliary RNA make the content of RNA in yeast mixture reach the 37% of total amount, add
Quality is the adjuvant of yeast mixture quality 4%, and adjuvant is formed according to the proportions that mass ratio is 1:1 by white sugar and glucose,
Controlling pH is 5.6, after stirring 15min, adds protease and controls after pH is 5.6 reaction 4h, then adds nucleic acid after being warming up to 60 DEG C
It is 5.1 reaction 10h that enzyme controls pH, then add transaminase to control pH to be 5.7 reaction 8h after being cooled to 45 DEG C, then temperature rises to 65 DEG C
Rear insulation 30min.The addition of protease is the 0.5% of total amount, the addition of nuclease is the 0.7% of total amount, transaminase
Addition is the 0.15% of total amount；
C, enzyme denaturing separate: the yeast mixture that self-dissolving enzymolysis completes is warming up to 87 DEG C and carries out enzyme denaturing process, and the process time is
32min, carries out centrifuging treatment after it cools down, and separating treatment rotating speed is 4500r/min, obtains residue after processing 30min
And clear liquid, residue is used for feedstuff and prepares；
D, hyperfiltration treatment: by the clear liquid temperature control of isolated to 52 DEG C, carry out hyperfiltration treatment under the pressure of 0.31MPa；
E, homogenizing concentrate: the clear liquid through hyperfiltration treatment is put in homogenizer homogenizing under the pressure of 6MPa, by homogenizing
The clear liquid temperature control completed makes, to 55 DEG C of concentrations, the semi-solid products that dry biomass mark is 35%；
F, spray drying: semi-solid products is sent into spray drying tower internal spraying and is dried prepared extract finished product, spray dried
Dry inlet temperature is 158 DEG C, and leaving air temp is 93 DEG C, and drying pressure is 13MPa, water content≤5% of extract finished product.
Embodiment three
A kind of preparation method of high I+G content yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain turn out protein content be 62%, rna content be 8.8%, sea
The high-protein yeast breast of algae sugar content≤4%, enters the high-protein yeast breast obtained tank and is heated to 94 DEG C and boils 70min, control pH
It is 6.8, is then cooled to 60 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters self-dissolving tank, to yeast mixture
Middle addition concentration is 53%, purity be 80% auxiliary RNA make the content of RNA in yeast mixture reach the 38% of total amount, add
Quality is the adjuvant of yeast mixture quality 4%, and adjuvant is formed according to the proportions of mass ratio 1:1 by white sugar and glucose, control
PH processed is 5.7, after stirring 20min, adds protease and controls after pH is 5.7 reaction 4h, then adds nuclease after being warming up to 62 DEG C
Controlling pH is 5.2 reaction 10h, then add transaminase to control pH to be 5.8 reaction 6h after being cooled to 46 DEG C, then after temperature is risen to 65 DEG C
Insulation 30min, the addition of protease is the 0.5% of total amount, the addition of nuclease is the 0.6% of total amount, the adding of transaminase
Enter that amount is total amount 0.15%；
C, enzyme denaturing separate: the yeast mixture that self-dissolving enzymolysis completes is warming up to 87 DEG C and carries out enzyme denaturing process, and the process time is
33min, carries out centrifuging treatment after it cools down, and separating treatment rotating speed is 5000r/min, obtains residue after processing 30min
And clear liquid, residue is used for feedstuff and prepares；
D, hyperfiltration treatment: by the clear liquid temperature control of isolated to 56 DEG C, carry out hyperfiltration treatment under the pressure of 0.33MPa；
E, homogenizing concentrate: the clear liquid through hyperfiltration treatment is put in homogenizer homogenizing under the pressure of 7MPa, by homogenizing
The clear liquid temperature control completed makes, to 55 DEG C of concentrations, the semi-solid products that dry biomass mark is 35%；
F, spray drying: semi-solid products is sent into spray drying tower internal spraying and is dried prepared extract finished product, spray dried
Dry inlet temperature is 158 DEG C, and leaving air temp is 93 DEG C, and drying pressure is 13MPa, water content≤5% of extract finished product.
Embodiment four
A kind of preparation method of high I+G content yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain turn out protein content be 61%, rna content be 8.7%, sea
The high-protein yeast breast of algae sugar content≤4%, enters the high-protein yeast breast obtained tank and is heated to 94 DEG C and boils 75min, control pH
It is 6.7, is then cooled to 58 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters self-dissolving tank, to yeast mixture
Middle addition concentration is 53%, purity be 80% auxiliary RNA make the content of RNA in yeast mixture reach the 38% of total amount, add
Quality is the adjuvant of yeast mixture quality 4%, and adjuvant is formed according to the proportions that mass ratio is 1:1 by white sugar and glucose,
Controlling pH is 5.8, after stirring 25min, adds protease and controls after pH is 5.6 reaction 4h, then adds nucleic acid after being warming up to 60 DEG C
It is 5.1 reaction 10h that enzyme controls pH, then add transaminase to control pH to be 5.7 reaction 8h after being cooled to 50 DEG C, then temperature rises to 65 DEG C
Rear insulation 30min, the addition of protease is the 0.5% of total amount, the addition of nuclease is the 0.7% of total amount, transaminase
Addition is the 0.15% of total amount；
C, enzyme denaturing separate: the yeast mixture that self-dissolving enzymolysis completes is warming up to 85-90 DEG C and carries out enzyme denaturing process, and the process time is
34min, carries out centrifuging treatment after it cools down, and separating treatment rotating speed is 4800r/min, obtains residue after processing 30min
And clear liquid, residue is used for feedstuff and prepares；
D, hyperfiltration treatment: by the clear liquid temperature control of isolated to 58 DEG C, carry out hyperfiltration treatment under the pressure of 0.34MPa；
E, homogenizing concentrate: the clear liquid through hyperfiltration treatment is put in homogenizer homogenizing under the pressure of 9MPa, by homogenizing
The clear liquid temperature control completed makes, to 55 DEG C of concentrations, the semi-solid products that dry biomass mark is 35%；
F, spray drying: semi-solid products is sent into spray drying tower internal spraying and is dried prepared extract finished product, spray dried
Dry inlet temperature is 158 DEG C, and leaving air temp is 93 DEG C, and drying pressure is 13MPa, water content≤5% of extract finished product.
Embodiment five
A kind of preparation method of high I+G content yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain turn out protein content be 62%, rna content be 8.8%, sea
The high-protein yeast breast of algae sugar content≤4%, enters the high-protein yeast breast obtained tank and is heated to 95 DEG C and boils 80min, control pH
It is 6.8, is then cooled to 65 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters self-dissolving tank, to yeast mixture
Middle addition concentration is 53%, purity be 80% auxiliary RNA make the content of RNA in yeast mixture reach the 39% of total amount, add
Quality is the adjuvant of yeast mixture quality 4%, and adjuvant is formed according to the proportions that mass ratio is 1:1 by white sugar and glucose,
Controlling pH is 5.9, after stirring 10-30min, adds protease and controls after pH is 5.7 reaction 4h, then adds core after being warming up to 62 DEG C
It is 5.2 reaction 10h that acid enzyme controls pH, then add transaminase to control pH to be 5.8 reaction 6h after being cooled to 48 DEG C, then temperature is risen to 65
Being incubated 30min after DEG C, the addition of protease is the 0.5% of total amount, the addition of nuclease is the 0.6% of total amount, transaminase
Addition is total amount 0.15%；
C, enzyme denaturing separate: the yeast mixture that self-dissolving enzymolysis completes is warming up to 90 DEG C and carries out enzyme denaturing process, and the process time is
35min, carries out centrifuging treatment after it cools down, and separating treatment rotating speed is 5000r/min, obtains residue after processing 30min
And clear liquid, residue is used for feedstuff and prepares；
D, hyperfiltration treatment: by the clear liquid temperature control of isolated to 60 DEG C, carry out hyperfiltration treatment under the pressure of 0.35MPa；
E, homogenizing concentrate: the clear liquid through hyperfiltration treatment is put in homogenizer homogenizing under the pressure of 10MPa, by homogenizing
The clear liquid temperature control completed makes, to 55 DEG C of concentrations, the semi-solid products that dry biomass mark is 35%；
F, spray drying: semi-solid products is sent into spray drying tower internal spraying and is dried prepared extract finished product, spray dried
Dry inlet temperature is 158 DEG C, and leaving air temp is 93 DEG C, and drying pressure is 13MPa, water content≤5% of extract finished product.
Below in conjunction with specific experiment example, the present invention is described further.
Experimental example 1
The high I+G content that the embodiment of the present invention one, embodiment two, embodiment three, embodiment four, embodiment five are prepared
Yeast extract carries out detecting, its testing result is as shown in table 1:
Table 1 high I+G content yeast extract testing result
The yeast extract that the present invention prepares as can be seen from the above table complies fully with standard, the high I+G content prepared
The I+G content desalination of yeast extract is given money as a gift between 19.5-21.0%, reaches desired value, improves economic worth and meet
The demand of people.
Claims (7)
1. the preparation method of one kind high I+G content yeast extract, it is characterised in that: the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain to turn out high-protein yeast breast, the high-protein yeast breast pH control that will obtain
It is made as 6-8；
B, self-dissolving enzymolysis: yeast milk separating treatment obtains yeast mixture, yeast mixture enters self-dissolving tank, adds auxiliary in yeast mixture
RNA makes the content of RNA in yeast mixture reach the 30-40% of total amount, adds adjuvant, protease, nuclease, transaminase, controls
PH carries out self-dissolving enzyme digestion reaction；
C, enzyme denaturing separate: temperature is raised enzyme denaturing and is then peeled off；
D, hyperfiltration treatment: the clear liquid of isolated is carried out hyperfiltration treatment；
E, homogenizing concentrate: putting in homogenizer by the clear liquid through hyperfiltration treatment, the clear liquid completed by homogenizing is condensed into semisolid
Product；
F, spray drying: semi-solid products is sent into spray drying tower internal spraying and is dried prepared extract finished product.
Preparation method the most according to claim 1, it is characterised in that the concentration of the auxiliary RNA in described step b is 53-
63%.
Preparation method the most according to claim 1, it is characterised in that the adjuvant in described step b is white sugar and Fructus Vitis viniferae
Sugar forms according to the proportions that mass ratio is 1:1.
Preparation method the most according to claim 1, it is characterised in that in described step b, the addition of protease is total amount
0.1-0.7%, the addition of nuclease be the 0.5-1.0% of total amount, the 0.05-that addition is total amount of transaminase
0.15%.
Preparation method the most according to claim 1, it is characterised in that in described step b, protease is papain, nothing
One in ficin, bromelain.
Preparation method the most according to claim 1, it is characterised in that in described step b, the self-dissolving enzymolysis response time is 16-
20h。
Preparation method the most according to claim 1, it is characterised in that the pH value control of self-dissolving enzyme digestion reaction in described step b
It is made as 4.5-6.8.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112515160A (en) * | 2020-12-25 | 2021-03-19 | 浙江东成生物科技股份有限公司 | Preparation method of yeast extract |
| WO2022135181A1 (en) * | 2020-12-23 | 2022-06-30 | 安琪酵母股份有限公司 | Preparation method for and application of soluble glucan-rich yeast extract |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022135181A1 (en) * | 2020-12-23 | 2022-06-30 | 安琪酵母股份有限公司 | Preparation method for and application of soluble glucan-rich yeast extract |
| CN112515160A (en) * | 2020-12-25 | 2021-03-19 | 浙江东成生物科技股份有限公司 | Preparation method of yeast extract |
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