CN105919137A - Preparation method of high-protein yeast extract - Google Patents
Preparation method of high-protein yeast extract Download PDFInfo
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- CN105919137A CN105919137A CN201610266633.6A CN201610266633A CN105919137A CN 105919137 A CN105919137 A CN 105919137A CN 201610266633 A CN201610266633 A CN 201610266633A CN 105919137 A CN105919137 A CN 105919137A
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- yeast
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- protein
- yeast extract
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- 229940041514 candida albicans extract Drugs 0.000 title claims abstract description 28
- 239000012138 yeast extract Substances 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 60
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000008267 milk Substances 0.000 claims abstract description 13
- 235000013336 milk Nutrition 0.000 claims abstract description 13
- 210000004080 milk Anatomy 0.000 claims abstract description 13
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 8
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims description 28
- 239000004365 Protease Substances 0.000 claims description 18
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 13
- 108091005804 Peptidases Proteins 0.000 claims description 12
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 12
- 235000019419 proteases Nutrition 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 11
- 101710163270 Nuclease Proteins 0.000 claims description 10
- 108090000340 Transaminases Proteins 0.000 claims description 10
- 102000003929 Transaminases Human genes 0.000 claims description 10
- 239000000284 extract Substances 0.000 claims description 9
- 239000002671 adjuvant Substances 0.000 claims description 7
- 210000000481 breast Anatomy 0.000 claims description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 108010004032 Bromelains Proteins 0.000 claims description 2
- 108090000270 Ficain Proteins 0.000 claims description 2
- 108090000526 Papain Proteins 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- 230000002358 autolytic effect Effects 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 235000019835 bromelain Nutrition 0.000 claims description 2
- 238000001976 enzyme digestion Methods 0.000 claims description 2
- 235000019836 ficin Nutrition 0.000 claims description 2
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 claims description 2
- 235000019834 papain Nutrition 0.000 claims description 2
- 229940055729 papain Drugs 0.000 claims description 2
- 239000002994 raw material Substances 0.000 abstract description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 4
- 208000035404 Autolysis Diseases 0.000 abstract 2
- 206010057248 Cell death Diseases 0.000 abstract 2
- 230000028043 self proteolysis Effects 0.000 abstract 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- 230000029219 regulation of pH Effects 0.000 abstract 1
- 238000010792 warming Methods 0.000 description 6
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 3
- 241000209051 Saccharum Species 0.000 description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000004885 white matter Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of a high-protein yeast extract. The method comprises the following steps of preparing yeast milk, performing autolysis and enzymolysis, performing enzymic inactivation and separation, performing ultrafiltration concentration, and performing homogenizing so as to obtain finished products. A conventional saccharomyces cerevisiae bacterial strain is used, so that the yeast milk of which the protein content can achieve 59% or above can be prepared; then the yeast milk with a high protein content is used as the raw material, and strict temperature control and pH regulation are performed, so that the autolysis and enzymolysis treatment is performed; in the treatment process, an auxiliary RNA is added, so that a foundation is established for the preparation of a yeast extract with a high I+G content; finally, supernate obtained through separation is subjected to the ultrafiltration concentration, so that the yeast extract of which the I+G content is 18% or above is obtained, and requirements of people are met, and the economic value is increased.
Description
Technical field
The present invention relates to yeast extract preparing technical field, be specifically related to a kind of high-protein yeast
The preparation method of extract.
Background technology
Along with the expansion year by year of yeast extract application, it is widely used in infant with old
The industries such as year people's food, nutrition enhancer, health product, although China is world's yeast extract
Big producing country, but its I+G content of the product generally prepared is only between 2%-20%, due to
I+G content more high value is the biggest, and the main cause affecting I+G content is albumen in yeast
The content of matter, in order to strengthen its nutrition and mouthfeel further, uses high egg in extract produces
White matter, the yeast raw material of high nucleic acid content, prepare the yeast extract that I+G content is higher,
To meet demand to Seasoning Ingredients after people's living standard improves.
The present inventor, by substantial amounts of experiment and research, provides a kind of I+G content energy for people
Enough reach the preparation method of the high-protein yeast extract of more than 18%, take out solving existing yeast
The problem that extract I+G content is relatively low.
Summary of the invention
For solving above-mentioned technical problem, the invention provides the system of a kind of high-protein yeast extract
Preparation Method.
The present invention is achieved by the following technical programs:
A kind of preparation method of high-protein yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain to turn out high-protein yeast breast, control
PH is 6.6-6.8；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment, yeast mixture enters self-dissolving tank,
Adding auxiliary RNA in yeast mixture makes the content of RNA in yeast mixture reach total amount
35-40%, adds adjuvant, and protease, nuclease, transaminase control pH for carrying out autolytic enzyme
Solve reaction；
C, enzyme denaturing separate: the yeast mixture completed by self-dissolving enzymolysis heats up and carries out enzyme denaturing process, so
After carry out centrifuging treatment and obtain residue and clear liquid, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid of isolated through hyperfiltration treatment, being condensed into moisture is
The product of 50-60%；
E, homogenizing finished product: the product obtained is put into homogenizing in homogenizer, is dried to water content
≤ 5% obtains extract finished product.
The protein content of the high-protein yeast breast in described step a is 59-61%.
The concentration of the auxiliary RNA in described step b is 53-65%.
Adjuvant in described step b is white sugar and glucose is the ratio of 2:1 according to mass ratio
Formulated.
In described step b, the addition of protease is the addition of the 0.1-0.5% of total amount, nuclease
Amount is the 0.5-0.9% of total amount, the 0.05-0.1% that addition is total amount of transaminase.
In described step b, protease is papain, ficin, bromelain
In one.
In described step b, the pH value of self-dissolving enzyme digestion reaction controls as 4.8-6.8.
In described step c, the separation rotating speed of centrifuging treatment is 3500-5000r/min, and the time is
30min。
The beneficial effects of the present invention is: the present invention utilizes existing Wine brewing yeast strain to prepare
Protein content can reach the yeast milk of more than 59%, then with the yeast milk of high protein content is
Raw material, carries out self-dissolving enzymolysis processing by strict temperature control, pH regulator, during processing
Carry out assisting the yeast extract being added to prepare high I+G content of RNA to lay a good foundation,
Finally by the clear liquid of isolated through ultrafiltration concentration process prepared I+G content reach 18% with
On yeast extract, meet the demand of people, improve economic worth.
Accompanying drawing explanation
Fig. 1 is the process chart of the present invention
Detailed description of the invention
Below in conjunction with drawings and Examples, technical scheme is further described, but wants
The scope asking protection is not limited to described.
Experimental example one
A kind of preparation method of high-protein yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain turn out protein content be 59%,
Rna content is 8.5%, the high-protein yeast of content of trehalose≤4% breast, the high egg that will obtain
White yeast milk enters tank and is heated to 90 DEG C and boils 60min, and controlling pH is 6.6, is then cooled to 65 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters
Enter self-dissolving tank, in yeast mixture add concentration be 53%, purity be 80% auxiliary RNA make
In yeast mixture, the content of RNA reaches the 35% of total amount, and adding quality is that yeast mixture quality 3% is white
Saccharum Sinensis Roxb. and glucose are according to the adjuvant of the proportions that mass ratio is 2:1, and controlling pH is
5.5, after stirring 10min, after addition protease control pH is 5.5 reaction 4h, then it is warming up to 68 DEG C
It is 5.0 reaction 10h that rear addition nuclease controls pH, then adds transaminase after being cooled to 50 DEG C and control pH
Being 5.6 reaction 6h, then be incubated 30min after temperature rises to 65 DEG C, the addition of protease is total
Amount 0.5%, the addition of nuclease be the 0.5% of total amount, the addition of transaminase be total amount
0.1%；
C, enzyme denaturing separate: the yeast mixture that self-dissolving enzymolysis completes is warming up to 80 DEG C and carries out at enzyme denaturing
Reason, the process time is 30min, carries out centrifuging treatment and obtain residue with clear after it cools down
Liquid, separation rotating speed is 3500r/min, and the time is 30min, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid temperature control of isolated to 50 DEG C, at the pressure of 0.3MPa
Lower through hyperfiltration treatment, it is condensed into the product that moisture is 50%；
E, homogenizing finished product: the product obtained is put into homogenizing in homogenizer, then at 80-90 DEG C
At a temperature of be dried to water content≤5% and obtain extract finished product.
Embodiment two
A kind of preparation method of high-protein yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain turn out protein content be 61%,
Rna content is 8.7%, the high-protein yeast of content of trehalose≤4% breast, the high egg that will obtain
White yeast milk enters tank and is heated to 92 DEG C and boils 100min, and controlling pH is 6.8, is then cooled to 65 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters
Enter self-dissolving tank, in yeast mixture add concentration be 53%, purity be 80% auxiliary RNA make
In yeast mixture, the content of RNA reaches the 37% of total amount, and adding quality is that yeast mixture quality 3% is white
Saccharum Sinensis Roxb. and glucose are according to the adjuvant of the proportions that mass ratio is 2:1, and controlling pH is
5.8, after stirring 30min, after addition protease control pH is 5.6 reaction 4h, then it is warming up to 68 DEG C
It is 5.1 reaction 10h that rear addition nuclease controls pH, then adds transaminase after being cooled to 50 DEG C and control pH
Being 5.7 reaction 6h, then be incubated 30min after temperature rises to 65 DEG C, the addition of protease is total
Amount 0.5%, the addition of nuclease be the 0.5% of total amount, the addition of transaminase be total amount
0.1%；
C, enzyme denaturing separate: the yeast mixture that self-dissolving enzymolysis completes is warming up to 90 DEG C and carries out at enzyme denaturing
Reason, the process time is 45min, carries out centrifuging treatment and obtain residue with clear after it cools down
Liquid, separation rotating speed is 5000r/min, and the time is 30min, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid temperature control of isolated to 60 DEG C, at the pressure of 0.35MPa
Lower through hyperfiltration treatment, it is condensed into the product that moisture is 60%；
E, homogenizing finished product: the product obtained is put into homogenizing in homogenizer, then the temperature at 90 DEG C
It is dried under degree to water content≤5% and obtains extract finished product.
Embodiment three
A kind of preparation method of high-protein yeast extract, the method comprises the following steps:
A, yeast milk prepare: use Wine brewing yeast strain turn out protein content be 60%,
Rna content is 8.6%, the high-protein yeast of content of trehalose≤4% breast, the high egg that will obtain
White yeast milk enters tank and is heated to 91 DEG C and boils 80min, and controlling pH is 6.7, is then cooled to 65 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters
Enter self-dissolving tank, in yeast mixture add concentration be 53%, purity be 80% auxiliary RNA make
In yeast mixture, the content of RNA reaches the 36% of total amount, and adding quality is that yeast mixture quality 3% is white
Saccharum Sinensis Roxb. and glucose are according to the adjuvant of the proportions that mass ratio is 2:1, and controlling pH is
5.7, after stirring 20min, after addition protease control pH is 5.5 reaction 4h, then it is warming up to 68 DEG C
It is 5.0 reaction 10h that rear addition nuclease controls pH, then adds transaminase after being cooled to 50 DEG C and control pH
Being 5.6 reaction 6h, then be incubated 30min after temperature rises to 65 DEG C, the addition of protease is total
Amount 0.5%, the addition of nuclease be the 0.5% of total amount, the addition of transaminase be total amount
0.1%；
C, enzyme denaturing separate: the yeast mixture that self-dissolving enzymolysis completes is warming up to 85 DEG C and carries out at enzyme denaturing
Reason, the process time is 37min, carries out centrifuging treatment and obtain residue with clear after it cools down
Liquid, separation rotating speed is 4500r/min, and the time is 30min, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid temperature control of isolated to 55 DEG C, at the pressure of 0.33MPa
Lower through hyperfiltration treatment, it is condensed into the product that moisture is 55%；
E, homogenizing finished product: the product obtained is put into homogenizing in homogenizer, then the temperature at 85 DEG C
It is dried under degree to water content≤5% and obtains extract finished product.
Below in conjunction with specific experiment example, the present invention is further illustrated.
Experimental example 1
The high-nucleic acid yeast preparing the embodiment of the present invention one, embodiment two, embodiment three is taken out
Extract carries out detecting, its testing result is as shown in table 1:
Table 1 high-nucleic acid yeast extract testing result
The yeast extract that the present invention prepares as can be seen from the above table complies fully with standard, preparation
The I+G content desalination of the high-nucleic acid yeast extract gone out is given money as a gift between 18.0-21.0%, reaches mesh
Scale value, improves economic worth and meets the demand of people.
Claims (8)
1. the preparation method of a high-protein yeast extract, it is characterised in that: the method bag
Include following steps:
A, yeast milk prepare: use Wine brewing yeast strain to turn out high-protein yeast breast, control
PH is 6.6-6.8；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment, yeast mixture enters self-dissolving tank,
Adding auxiliary RNA in yeast mixture makes the content of RNA in yeast mixture reach total amount
35-40%, adds adjuvant, and protease, nuclease, transaminase control pH for carrying out autolytic enzyme
Solve reaction；
C, enzyme denaturing separate: the yeast mixture completed by self-dissolving enzymolysis heats up and carries out enzyme denaturing process, so
After carry out centrifuging treatment and obtain residue and clear liquid, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid of isolated through hyperfiltration treatment, being condensed into moisture is
The product of 50-60%；
E, homogenizing finished product: the product obtained is put into homogenizing in homogenizer, is dried to water content
≤ 5% obtains extract finished product.
The preparation method of high-protein yeast extract the most according to claim 1, its feature
It is: the protein content of the high-protein yeast breast in described step a is 59-61%.
The preparation method of high-protein yeast extract the most according to claim 1, its feature
It is: the concentration of the auxiliary RNA in described step b is 53-65%.
The preparation method of high-protein yeast extract the most according to claim 1, its feature
It is: the adjuvant in described step b is that white sugar and glucose are according to the ratio that mass ratio is 2:1
Example is formulated.
The preparation method of high-protein yeast extract the most according to claim 1, its feature
Be: in described step b the addition of protease be the 0.1-0.5% of total amount, the adding of nuclease
Enter amount be the 0.5-0.9% of total amount, the 0.05-0.1% that addition is total amount of transaminase.
The preparation method of high-protein yeast extract the most according to claim 1, its feature
It is: in described step b, protease is papain, ficin, bromelain
One in enzyme.
The preparation method of high-protein yeast extract the most according to claim 1, its feature
It is: in described step b, the pH value of self-dissolving enzyme digestion reaction controls as 4.8-6.8.
The preparation method of high-protein yeast extract the most according to claim 1, its feature
It is: in described step c, the separation rotating speed of centrifuging treatment is 3500-5000r/min, the time
For 30min.
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| CN201610266633.6A CN105919137A (en) | 2016-04-27 | 2016-04-27 | Preparation method of high-protein yeast extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610266633.6A CN105919137A (en) | 2016-04-27 | 2016-04-27 | Preparation method of high-protein yeast extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105919137A true CN105919137A (en) | 2016-09-07 |
Family
ID=56837259
Family Applications (1)
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| CN201610266633.6A Pending CN105919137A (en) | 2016-04-27 | 2016-04-27 | Preparation method of high-protein yeast extract |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108185388A (en) * | 2017-12-28 | 2018-06-22 | 天津百利食品有限公司 | A kind of method that low sodium yeast extract is prepared using radio-frequency technique |
| CN110403066A (en) * | 2018-04-26 | 2019-11-05 | 安琪酵母(崇左)有限公司 | Feeding nucleotidic yeast hydrolyate and its preparation method and application |
| CN112515160A (en) * | 2020-12-25 | 2021-03-19 | 浙江东成生物科技股份有限公司 | Preparation method of yeast extract |
| CN112535280A (en) * | 2020-12-25 | 2021-03-23 | 浙江东成生物科技股份有限公司 | Preparation method of yeast extract with high protein content |
| CN113862164A (en) * | 2021-12-02 | 2021-12-31 | 中国食品发酵工业研究院有限公司 | High-protein saccharomyces cerevisiae and application thereof |
| WO2022135181A1 (en) * | 2020-12-23 | 2022-06-30 | 安琪酵母股份有限公司 | Preparation method for and application of soluble glucan-rich yeast extract |
| CN116138446A (en) * | 2023-02-16 | 2023-05-23 | 广州市肽汇生物科技有限公司 | Yeast extract product for improving delicate flavor and preparation method thereof |
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| CN101756151B (en) * | 2008-12-24 | 2012-12-12 | 安琪酵母股份有限公司 | Yeast extract with high glutamic acid content and preparation method thereof |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108185388A (en) * | 2017-12-28 | 2018-06-22 | 天津百利食品有限公司 | A kind of method that low sodium yeast extract is prepared using radio-frequency technique |
| CN110403066A (en) * | 2018-04-26 | 2019-11-05 | 安琪酵母(崇左)有限公司 | Feeding nucleotidic yeast hydrolyate and its preparation method and application |
| WO2022135181A1 (en) * | 2020-12-23 | 2022-06-30 | 安琪酵母股份有限公司 | Preparation method for and application of soluble glucan-rich yeast extract |
| CN114732127A (en) * | 2020-12-23 | 2022-07-12 | 安琪酵母(柳州)有限公司 | Preparation method and application of yeast extract rich in soluble glucan |
| CN112515160A (en) * | 2020-12-25 | 2021-03-19 | 浙江东成生物科技股份有限公司 | Preparation method of yeast extract |
| CN112535280A (en) * | 2020-12-25 | 2021-03-23 | 浙江东成生物科技股份有限公司 | Preparation method of yeast extract with high protein content |
| CN113862164A (en) * | 2021-12-02 | 2021-12-31 | 中国食品发酵工业研究院有限公司 | High-protein saccharomyces cerevisiae and application thereof |
| WO2023098678A1 (en) * | 2021-12-02 | 2023-06-08 | 安琪酵母股份有限公司 | High-protein saccharomyces cerevisiae and application thereof |
| CN116138446A (en) * | 2023-02-16 | 2023-05-23 | 广州市肽汇生物科技有限公司 | Yeast extract product for improving delicate flavor and preparation method thereof |
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