CN105942528A - Method for preparing high-nucleic-acid yeast extract - Google Patents
Method for preparing high-nucleic-acid yeast extract Download PDFInfo
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- CN105942528A CN105942528A CN201610268178.3A CN201610268178A CN105942528A CN 105942528 A CN105942528 A CN 105942528A CN 201610268178 A CN201610268178 A CN 201610268178A CN 105942528 A CN105942528 A CN 105942528A
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- China
- Prior art keywords
- yeast
- yeast extract
- preparation
- nucleic acid
- total amount
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 229940041514 candida albicans extract Drugs 0.000 title claims abstract description 27
- 239000012138 yeast extract Substances 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 22
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 56
- 238000002360 preparation method Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 235000013336 milk Nutrition 0.000 claims abstract description 10
- 239000008267 milk Substances 0.000 claims abstract description 10
- 210000004080 milk Anatomy 0.000 claims abstract description 10
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 9
- 238000000926 separation method Methods 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims description 28
- 239000004365 Protease Substances 0.000 claims description 16
- 238000006243 chemical reaction Methods 0.000 claims description 13
- 101710163270 Nuclease Proteins 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 10
- 108090000340 Transaminases Proteins 0.000 claims description 10
- 102000003929 Transaminases Human genes 0.000 claims description 10
- 235000019419 proteases Nutrition 0.000 claims description 10
- 210000000481 breast Anatomy 0.000 claims description 8
- 239000000284 extract Substances 0.000 claims description 8
- 238000010792 warming Methods 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 239000002671 adjuvant Substances 0.000 claims description 7
- 229940088598 enzyme Drugs 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical group O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 5
- 229930006000 Sucrose Natural products 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000001976 enzyme digestion Methods 0.000 claims description 4
- 108010004032 Bromelains Proteins 0.000 claims description 2
- 108090000270 Ficain Proteins 0.000 claims description 2
- 108090000526 Papain Proteins 0.000 claims description 2
- 235000019835 bromelain Nutrition 0.000 claims description 2
- 235000019836 ficin Nutrition 0.000 claims description 2
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- 235000019834 papain Nutrition 0.000 claims description 2
- 229940055729 papain Drugs 0.000 claims description 2
- 239000002994 raw material Substances 0.000 abstract description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 4
- 230000002358 autolytic effect Effects 0.000 abstract 2
- 230000029219 regulation of pH Effects 0.000 abstract 1
- 241000195493 Cryptophyta Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a method for preparing a high-nucleic-acid yeast extract. The method comprises the following steps of yeast milk preparation, autolytic enzymolysis, separation, ultrafiltration concentration, and finished product obtaining. Yeast milk with the protein content reaching 59% or more is prepared by using a conventional saccharomyces cerevisiae strain, and then the yeast milk with high protein content is used as the raw material; autolytic enzymolysis treatment is performed through strict temperature control and pH regulation; addition of an auxiliary RNA in the treating process lays a foundation for preparation of the yeast extract with high I+G content; and finally, a clear liquid obtained from separation is subjected to ultrafiltration concentration treatment to obtain the yeast extract with the I+G content reaching 17% or more. People needs are met, and the economic value is enhanced.
Description
Technical field
The present invention relates to yeast extract preparing technical field, be specifically related to the preparation side of a kind of high-nucleic acid yeast extract
Method.
Background technology
Along with the expansion year by year of yeast extract application, it is widely used in infant and food for the aged, nutrition
The industry such as hardening agent, health product, although China is yeast extract big producing country of the world, but its I+ of the product generally prepared
G content is only between 2%-20%, and owing to I+G content more high value is the biggest, and the main cause affecting I+G content is ferment
The content of protein in mother, in order to strengthen its nutrition and mouthfeel further, uses high protein, high nucleic acid in extract produces
The yeast raw material of content, prepares the yeast extract that I+G content is higher, former to seasoning to meet after people's living standard improves
The demand of material.
The present inventor, by substantial amounts of experiment and research, provides a kind of I+G content for people and can reach more than 17%
The preparation method of high-nucleic acid yeast extract, the problem relatively low to solve existing yeast extract I+G content.
Summary of the invention
For solving above-mentioned technical problem, the invention provides the preparation method of a kind of high-nucleic acid yeast extract.
The present invention is achieved by the following technical programs:
A kind of preparation method of high-nucleic acid yeast extract, the method comprises the following steps:
A, yeast milk prepare: using Wine brewing yeast strain turn out high-protein yeast breast to control pH is 6.6-7.1；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment, yeast mixture enters self-dissolving tank, adds auxiliary in yeast mixture
RNA makes the content of RNA in yeast mixture reach the 35-50% of total amount, adds adjuvant, and protease, nuclease, transaminase control pH
For carrying out self-dissolving enzyme digestion reaction；
C, separation: the yeast mixture that self-dissolving enzymolysis completes being warming up to 90 DEG C and carries out enzyme denaturing process, the process time is 25-
35min, then after separating treatment, obtain residue and clear liquid, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid of isolated through hyperfiltration treatment, be condensed into the paste that moisture is 25-30% and produce
Product；
E, finished product: the creamy product obtained is dried to water content≤5% and obtains extract finished product.
The concentration of the auxiliary RNA in described step b is 53-60%.
Adjuvant in described step b is white sugar and glucose forms according to the proportions that mass ratio is 2:1.
In described step b the addition of protease be the 0.2-0.4% of total amount, the 0.3-that addition is total amount of nuclease
0.5%, the addition of transaminase is the 0.08-0.1% of total amount.
One during protease is papain, ficin, bromelain in described step b.
In described step b, the pH value of self-dissolving enzyme digestion reaction controls as 5-7.
The beneficial effects of the present invention is: the present invention utilizes existing Wine brewing yeast strain to prepare protein content can
Reach the yeast milk of more than 59%, then with the yeast milk of high protein content as raw material, by strict temperature control, pH regulator carry out from
Molten enzymolysis processing, has carried out assisting the yeast extract being added to prepare high I+G content of RNA to establish during processing
Basis, finally processes the clear liquid of isolated through ultrafiltration concentration and has prepared I+G content and reach the yeast of more than 17% and take out
Extract, meets the demand of people, improves economic worth.
Accompanying drawing explanation
Fig. 1 is the process chart of the present invention
Detailed description of the invention
Below in conjunction with drawings and Examples, technical scheme is further described, but claimed scope is also
It is not limited to described.
Embodiment one
A kind of preparation method of high-nucleic acid yeast extract, the method comprises the following steps:
A, yeast milk prepare: using Wine brewing yeast strain to turn out protein content is 59%, and rna content is 8.3%, sea
The high-protein yeast breast of algae sugar content≤5%, enters the high-protein yeast breast obtained tank and is heated to 80 DEG C and boils 60min, control pH
It is 6.6, is then cooled to 65 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters self-dissolving tank, to yeast mixture
Middle addition concentration is 53%, purity be 80% auxiliary RNA make the content of RNA in yeast mixture reach the 35% of total amount, add
Quality be yeast mixture quality 3% by white sugar and glucose according to the adjuvant of the proportions that mass ratio is 2:1, control
PH is 5.5, after stirring 10min, adds protease and controls after pH is 5.5 reaction 4h, then adds nuclease control after being warming up to 68 DEG C
PH processed is 5.0 reaction 10h, then add transaminase to control pH to be 5.6 reaction 6h after being cooled to 50 DEG C, then protects after temperature rises to 65 DEG C
Temperature 30min, the addition of protease is the 0.4% of total amount, the addition of nuclease is the 0.5% of total amount, the addition of transaminase
Amount is the 0.1% of total amount；
C, separation: the yeast mixture that self-dissolving enzymolysis completes being warming up to 90 DEG C and carries out enzyme denaturing process, the process time is 25min,
After separating treatment, obtain residue and clear liquid again, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid temperature control of isolated to 42 DEG C, through hyperfiltration treatment under the pressure of 0.3MPa, dense
Shorten the creamy product that moisture is 25% into；
E, finished product: the creamy product obtained is dried at a temperature of 80 DEG C to water content≤5% and obtains extract finished product.
Embodiment two
A kind of preparation method of high-nucleic acid yeast extract, the method comprises the following steps:
A, yeast milk prepare: using Wine brewing yeast strain to turn out protein content is 61%, and rna content is 8.7%, sea
The high-protein yeast breast of algae sugar content≤5%, enters the high-protein yeast breast obtained tank and is heated to 92 DEG C and boils 120min, control pH
It is 6.8, is then cooled to 65 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters self-dissolving tank, to yeast mixture
Middle addition concentration is 53%, purity be 80% auxiliary RNA make the content of RNA in yeast mixture reach the 37% of total amount, add
Quality be yeast mixture quality 3% by white sugar and glucose according to the adjuvant of the proportions that mass ratio is 2:1, control
PH is 5.8, after stirring 30min, adds protease and controls after pH is 5.6 reaction 4h, then adds nuclease control after being warming up to 68 DEG C
PH processed is 5.1 reaction 10h, then add transaminase to control pH to be 5.7 reaction 6h after being cooled to 50 DEG C, then protects after temperature rises to 65 DEG C
Temperature 30min, the addition of protease is the 0.4% of total amount, the addition of nuclease is the 0.5% of total amount, the addition of transaminase
Amount is the 0.1% of total amount；
C, separation: the yeast mixture that self-dissolving enzymolysis completes being warming up to 90 DEG C and carries out enzyme denaturing process, the process time is 35min,
After separating treatment, obtain residue and clear liquid again, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid temperature control of isolated to 45 DEG C, through hyperfiltration treatment under the pressure of 0.35MPa,
It is condensed into the creamy product that moisture is 30%；
E, finished product: the creamy product obtained is dried at a temperature of 90 DEG C to water content≤5% and obtains extract finished product.
Embodiment three
A kind of preparation method of high-nucleic acid yeast extract, the method comprises the following steps:
A, yeast milk prepare: using Wine brewing yeast strain to turn out protein content is 60%, and rna content is 8.5%, sea
The high-protein yeast breast of algae sugar content≤5%, enters the high-protein yeast breast obtained tank and is heated to 86 DEG C and boils 90min, control pH
It is 6.7, is then cooled to 65 DEG C；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment after having lowered the temperature, yeast mixture enters self-dissolving tank, to yeast mixture
Middle addition concentration is 53%, purity be 80% auxiliary RNA make the content of RNA in yeast mixture reach the 36% of total amount, add
Quality be yeast mixture quality 3% by white sugar and glucose according to the adjuvant of the proportions that mass ratio is 2:1, control
PH is 5.6, after stirring 10-30min, adds protease and controls after pH is 5.5 reaction 4h, then adds nuclease after being warming up to 68 DEG C
Controlling pH is 5.0 reaction 10h, then add transaminase to control pH to be 5.6 reaction 6h after being cooled to 50 DEG C, then after temperature is risen to 65 DEG C
Insulation 30min, the addition of protease is the 0.4% of total amount, the addition of nuclease is the 0.5% of total amount, the adding of transaminase
Enter that amount is total amount 0.1%；
C, separation: the yeast mixture that self-dissolving enzymolysis completes being warming up to 90 DEG C and carries out enzyme denaturing process, the process time is 30min,
After separating treatment, obtain residue and clear liquid again, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid temperature control of isolated to 43.5 DEG C, under the pressure of 0.32MPa at ultrafiltration
Reason, is condensed into the creamy product that moisture is 27.5%；
E, finished product: the creamy product obtained is dried at a temperature of 85 DEG C to water content≤5% and obtains extract finished product.
Below in conjunction with specific experiment example, the present invention is further illustrated.
Experimental example 1
The high-nucleic acid yeast extract preparing the embodiment of the present invention one, embodiment two, embodiment three detects, it
Testing result is as shown in table 1:
Table 1 high-nucleic acid yeast extract testing result
The yeast extract that the present invention prepares as can be seen from the above table complies fully with standard, the high-nucleic acid yeast prepared
The I+G content desalination of extract is given money as a gift between 17.0-21.0%, reaches desired value, improves economic worth and meet people
Demand.
Claims (6)
1. the preparation method of a high-nucleic acid yeast extract, it is characterised in that: the method comprises the following steps:
A, yeast milk prepare: using Wine brewing yeast strain turn out high-protein yeast breast to control pH is 6.6-7.1；
B, self-dissolving enzymolysis: obtain yeast mixture through separating treatment, yeast mixture enters self-dissolving tank, adds auxiliary RNA in yeast mixture
Making the content of RNA in yeast mixture reach the 35-50% of total amount, add adjuvant, protease, nuclease, transaminase control pH and are
Carry out self-dissolving enzyme digestion reaction；
C, separation: the yeast mixture that self-dissolving enzymolysis completes being warming up to 90 DEG C and carries out enzyme denaturing process, the process time is 25-35min, then
After separating treatment, obtain residue and clear liquid, residue is used for feedstuff and prepares；
D, ultrafiltration concentration: by the clear liquid of isolated through hyperfiltration treatment, be condensed into the creamy product that moisture is 25-30%；
E, finished product: the creamy product obtained is dried to water content≤5% and obtains extract finished product.
The preparation method of high-nucleic acid yeast extract the most according to claim 1, it is characterised in that: in described step b
The concentration of auxiliary RNA is 53-60%.
The preparation method of high-nucleic acid yeast extract the most according to claim 1, it is characterised in that: in described step b
Adjuvant is white sugar and glucose forms according to the proportions that mass ratio is 2:1.
The preparation method of high-nucleic acid yeast extract the most according to claim 1, it is characterised in that: egg in described step b
The addition of white enzyme is the 0.2-0.4% of total amount, the addition that addition is the 0.3-0.5% of total amount, transaminase of nuclease
0.08-0.1% for total amount.
The preparation method of high-nucleic acid yeast extract the most according to claim 1, it is characterised in that: egg in described step b
White enzyme is the one in papain, ficin, bromelain.
The preparation method of high-nucleic acid yeast extract the most according to claim 1, it is characterised in that: in described step b certainly
The pH value of molten enzyme digestion reaction controls as 5-7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610268178.3A CN105942528A (en) | 2016-04-27 | 2016-04-27 | Method for preparing high-nucleic-acid yeast extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610268178.3A CN105942528A (en) | 2016-04-27 | 2016-04-27 | Method for preparing high-nucleic-acid yeast extract |
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| CN105942528A true CN105942528A (en) | 2016-09-21 |
Family
ID=56915829
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|---|---|---|---|
| CN201610268178.3A Pending CN105942528A (en) | 2016-04-27 | 2016-04-27 | Method for preparing high-nucleic-acid yeast extract |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107373601A (en) * | 2017-09-01 | 2017-11-24 | 成都珪食品开发股份有限公司 | A kind of pig flesh flavor material and its formula, preparation method |
| CN112535280A (en) * | 2020-12-25 | 2021-03-23 | 浙江东成生物科技股份有限公司 | Preparation method of yeast extract with high protein content |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02219560A (en) * | 1989-02-22 | 1990-09-03 | Sanyo Kokusaku Pulp Co Ltd | Preparation of yeast extract having improved quality of taste |
| CN101513248A (en) * | 2008-02-19 | 2009-08-26 | 安琪酵母股份有限公司 | Yeast extract containing inosinic acid disodium salt and guanylic acid disodium and method for preparing same |
| CN101756151A (en) * | 2008-12-24 | 2010-06-30 | 安琪酵母股份有限公司 | Yeast extract with high glutamic acid content and preparation method thereof |
| CN102051381A (en) * | 2010-11-19 | 2011-05-11 | 广东一品鲜生物科技有限公司 | Method for preparing yeast extract by using beer yeast |
-
2016
- 2016-04-27 CN CN201610268178.3A patent/CN105942528A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02219560A (en) * | 1989-02-22 | 1990-09-03 | Sanyo Kokusaku Pulp Co Ltd | Preparation of yeast extract having improved quality of taste |
| CN101513248A (en) * | 2008-02-19 | 2009-08-26 | 安琪酵母股份有限公司 | Yeast extract containing inosinic acid disodium salt and guanylic acid disodium and method for preparing same |
| CN101756151A (en) * | 2008-12-24 | 2010-06-30 | 安琪酵母股份有限公司 | Yeast extract with high glutamic acid content and preparation method thereof |
| CN102051381A (en) * | 2010-11-19 | 2011-05-11 | 广东一品鲜生物科技有限公司 | Method for preparing yeast extract by using beer yeast |
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| Title |
|---|
| 吴丽云: "《啤酒废弃物为原料的Bt发酵研究》", 30 November 2011, 中国林业出版社 * |
| 李库等: "特鲜型酵母抽提物的开发与应用", 《中国食品添加剂》 * |
| 蔡奕文: "酵母抽提物生产工艺的优化", 《食品与机械》 * |
| 龚大春 等: "《酵母生物学》", 31 October 2015, 中国水利水电出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107373601A (en) * | 2017-09-01 | 2017-11-24 | 成都珪食品开发股份有限公司 | A kind of pig flesh flavor material and its formula, preparation method |
| CN112535280A (en) * | 2020-12-25 | 2021-03-23 | 浙江东成生物科技股份有限公司 | Preparation method of yeast extract with high protein content |
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Application publication date: 20160921 |