CN105941871A - Compound mouldproof additive for alfalfa hay - Google Patents
Compound mouldproof additive for alfalfa hay Download PDFInfo
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- CN105941871A CN105941871A CN201610364000.9A CN201610364000A CN105941871A CN 105941871 A CN105941871 A CN 105941871A CN 201610364000 A CN201610364000 A CN 201610364000A CN 105941871 A CN105941871 A CN 105941871A
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Abstract
The invention relates to a compound mouldproof additive for alfalfa hay, which is prepared from the following components in parts by mass: 10 to 30 parts of eucommia extract, 10 to 60 parts of allicin, 10 to 80 parts of ground cinnamon, 1.32 to 4 parts of potassium sorbate, 5.28 to 8 parts of sodium diacetate, and 26.4 to 40 parts of montmorillonite, when the compound mouldproof additive is used, the moisture content of the alfalfa hay is required to be 30 percent or below, 3 to 4.5kg of compound mouldproof additives are uniformly added into each ton of alfalfa hay. The compound mouldproof additive for the alfalfa hay can reduce the rising rate of temperature of bales in beginning of the storage of the alfalfa hay, reduce the temperature peak value of the bales, inhibit the growth of harmful microorganisms such as mould and the like, alfalfa can be safely stored for 60 days or more in the condition of high water content, the alfalfa hay is good in nutritional quality after being stored, and the plant source components have the effects of enhancing animal body immunity. The compound mouldproof additive for the alfalfa hay provided by the invention is simple in preparation method, low in cost, high in benefit, free from side effects, and more green and safe.
Description
Technical field
The present invention relates to forage feed processing technique field, be specifically related to a kind of alfalfa hay composite mildewproof and add
Agent.
Background technology
The Baling at high moisture storage of herbage is the effective measure reducing Pasture nutrient loss, but, if herbage water
Dividing too high, storage is because the activity of microorganism also results in nutrient loss, therefore, modulated in herbage
Cheng Zhong, can add herbage preservative to suppress growth and the breeding of microorganism.The kind of herbage preservative
Class is a lot, mainly based on chemicals such as organic acid, organic acid salt, ammonium compounds, carbamide,
The research of natural medicine and compound formulation in recent years gradually increases, the research and development of preservative towards convenient,
High-quality, efficiently, low cost and have no side effect, the direction of noresidue is developed.
Micro-life in organic acid substance can suppress herbage storage period is added when herbage Baling at high moisture is preserved
The activity of thing, it is to apply more additive types currently on the market that phenoxy propionic acid is combined herbage mildewproof additive,
Phenoxy propionic acid preparation has preferable anti-mold effect, and allows herbage bundling when water content 30% can realize peace
Full storage, but organic acid preparation has stronger corrosiveness to the metal part of machinery, and herbage operates machine
The tool market price is the most somewhat expensive, therefore uses organic acid as increasing herbage enterprise in actual production
Cost, additionally, because the usual storage time of herbage is the most long, and propanoic acid has certain volatility, with
The minimizing of propanoic acid molecules quantity, the effect of its mildew-resistant also can progressively decline.
Organic acid salt material such as sodium diacetate, potassium sorbate etc. all have extensively with feedstuff industry at food, grain
General utilization, but the mildew-resistant principle of acylate is identical with organic acid, has corruption the most equally
Erosion effect, and animal physiology is also had and causes potential harmful effect by the preservative of chemical industry synthesis.
Ammonium compounds can effectively suppress the breeding of major part noxious bacteria, reduces temperature in bale, reduces heat
Evil.There is slight alkalinization simultaneously, improve the quality and digestibility of herbage, but ammonium compounds exists
Being often accompanied by strong impulse abnormal smells from the patient during use, and conventional anhydrous ammonia is because having stronger volatility, it is prevented
Mould effect generally by 48h to several weeks, the time is the shortest.
Carbamide is as a kind of common nitrogenous fertilizer and feed additive, by being present in the urine in microorganism and plant
Element enzyme hydrolysis is ammonia, can efficiently control the growth of mycete, decreases heat evil, keeps good color and luster, and
When the content of ammonia arrives finite concentration, can promote that hemicellulose is broken down into simple material, thus reduce
The content of neutral detergent fiber.But, the process of carbamide release ammonia, extremely rely in herbage and urine can be provided
The quantity of the specific monoid microorganism of element enzyme, its effect is unstable.
Nowadays, Natural anti-mildew thing Quality Research is more concentrates on vegetable source natural antifungus agent, and it is with green, ring
Guarantor and the feature to person poultry safety are increasingly subject to the attention of people.Vegetable source natural antifungus agent includes that the Cortex Eucommiae carries
Taking thing, garlicin, citrus plant extract, Pericarpium Citri Reticulatae etc., this type of material possesses broad-spectrum antimicrobial effect more,
The antifungal mechanism of antipathogenic composition is the most complex, and every kind of plant component is generally directed to certain fungi flora to be had
Prominent inhibition.Compared with chemosynthesis material, pure natural substance active constituent content is relatively low, typically needs
Purify, but, the antibacterial efficiency of effective ingredient in natural materials is the highest, and have green, safety
Feature.
Advantage for comprehensive different mildew-resistant compositions, it is achieved more preferably anti-mold effect, the trial of composite mildewproof additive
Gradually increase, but the composite mildewproof additive component nowadays reported mostly be natural compound with natural materials and
It is combined between chemistry and chemical substance, and vegetable source natural material and the compound of chemical composition there are no report.
Summary of the invention
For solving problem above, the invention provides a kind of alfalfa hay composite mildewproof additive.
The alfalfa hay composite mildewproof additive that the present invention provides, is made up of the component of following mass parts:
Preferably, alfalfa hay composite mildewproof additive of the present invention, it is made up of the component of following mass parts:
Further, described Cortex Eucommiae extract is with the Cortex Eucommiae for extracting raw material, with the mixed solution extraction of alcohol with water
Prepare, chlorogenic acid content >=10%.
Further, described garlicin is artificial extraction or synthetic, is mainly composed of trithio allyl ethers
Compound.
Further, described potassium sorbate is that content is more than >=98% food-class high-purity additive.
Further, described sodium diacetate is that content is more than >=98% food-class high-purity additive.
Further, described montmorillonite is sodium montmorillonite or ca-montmorillonite.
Alfalfa hay composite mildewproof additive of the present invention alfalfa hay preserve in application time, it is desirable to lucerne
Mu Radix Glycyrrhizae moisture is 30% and less, and alfalfa hay per ton uniformly adds 3～4.5kg composite mildewproofs and adds
Agent.
The mouldy common flora of alfalfa hay includes main general character flora (aspergillus, Penicillium) present on herbage
With secondary general character flora (trichoderma, Alternaria, Fusarium, Fusarium), between various floras
There is synergism, single antifungus agent can be produced antagonistic effect, make fungicide effectiveness step-down, therefore use multiple
Closing antifungus agent is future research trends.In the present invention, Cortex Eucommiae extract correspondence trichoderma, garlicin is corresponding
Alternaria, Cortex Cinnamomi powder correspondence Fusarium, potassium sorbate+sodium diacetate correspondence Fusarium, these 5 kinds
Raw material all has good inhibitory action to aspergillus and Penicillium, and potassium sorbate+sodium diacetate is chemistry system
Agent, but addition is little also the effective ingredient in Cortex Eucommiae extract, garlicin and Cortex Cinnamomi powder is played protection
Effect, thus enhance effect of plant source antifungus agent, and montmorillonite is as additive substrate, its effect is
Absorb mineral nutrition in residual moisture in Radix Glycyrrhizae, absorption fungus body and toxin, increase Radix Glycyrrhizae, these compositions
Combine, alfalfa hay is had more antimildew and antibacterial effect targetedly.Wherein portfolio ratio is crucial,
Because the most not studying the various flora mechanism of action disclosed when alfalfa hay goes mouldy, therefore the present invention is by inciting somebody to action
The mixing of various component different proportions, then tied up by laboratory and alfalfa hay and test, it is determined that suitable
Best complex antifungus agent adding proportion on alfalfa hay.Comprehensive each side reason and result of the test, this
Invention preferably goes out optimal antifungus agent and combines: Cortex Eucommiae extract 20, garlicin 20, Cortex Cinnamomi powder 27, potassium sorbate
1.32, sodium diacetate 5.28, montmorillonite 2 6.4.
The invention have the benefit that
1. non-corrosiveness, stable in properties is non-volatile, broad-spectrum antimicrobial effect good: the present invention provide alfalfa hay
Composite mildewproof additive, by vegetable source natural material (Cortex Eucommiae extract, garlicin, Cortex Cinnamomi powder) and chemistry
Preparation (sodium diacetate, potassium sorbate), antifungus agent substrate (montmorillonite) form, and are particularly suitable for preserving
Containing the alfalfa hay of 20%-30% (mass percent) moisture, chemicals addition is smaller, corrosion-free
Domestic animal health also will not be had undesirable effect by property.In actual applications, composite mildewproof additive can reduce dry
The harmful microorganisms such as grass storage initial stage bale temperature rate-of-rise, reduces bale temperature peak, suppression mycete are raw
Long, Herba Medicaginis safe storage more than 60 days under the conditions of high-moisture (28%) can be made.
2. after storage, Radix Glycyrrhizae nutritional quality is more preferable: the alfalfa hay composite mildewproof additive of the present invention, has many
The antibacterial principle of weight is intersected collaborative, if different kinds of molecules is to the infiltration of microbial cell and interference and moisture and fungus
Physisorption, compare other antifungus agents and more can effectively reduce the quantity of mycete and the activity of mycete, from
And make Radix Glycyrrhizae retain the nutritional labelings such as more crude protein, soluble sugar in storage, and montmorillonite
Also can increase the content of mineral substances in Radix Glycyrrhizae, make up the deficiency of the mineral element of Radix Glycyrrhizae own.
3. enhancing domestic animal immunity: the vegetable-derived components that the alfalfa hay composite mildewproof additive of the present invention uses
Cortex Eucommiae extract, garlicin and Cortex Cinnamomi element itself also have the effect strengthening animal body immunity.
The most inexpensive, green safety: the mildew-resistant composite additive agent preparation method of the present invention is simple, low cost,
High efficiency, have no side effect, more green safety, be thus suitable for large-scale popularization and application.
Detailed description of the invention
Technical scheme is further illustrated below by way of specific embodiment.
Embodiment 1: each component proportion Preliminary screening
According to collaborative, interaction effect between raw material, design theory has good all of bacteria mildew-proof effect and joins
Than (totally 16 proportioning combinations).Wherein Hay Armer is that the industry of external import commonly uses Herba Medicaginis antifungus agent product
Kind；Cortex Eucommiae extract is produced by Yuquan, Shaanxi bio tech ltd, chlorogenic acid content >=10%；Cover de-
Stone is that Ningcheng County Tian Yuan montmorillonite development corporation, Ltd. produces.Every kind of antifungus agent component proportion is as shown in table 1,
Make 16 kinds of Radix Glycyrrhizae mildewproof additives, be stored at room temperature.
Table 1, the additive formula of confession Preliminary screening
It is raw material with mouldy hay, the 1:10000 extension rate mycete diluent of preparation.Preparation method is as follows:
Weigh the alfalfa hay 25g of mycete, put in the 250ml triangular flask containing 225ml sterile saline,
Put vibration 30min on agitator.With the above-mentioned diluent of 10ml suction pipe absorption 10ml 1:10 in 25ml test tube,
With ear washing bulb and pressure-vaccum 50 times repeatedly of 1ml suction pipe, mycotic spore is made to scatter.Take this diluent of 1ml and add 9ml
Normal saline, fully mixes to obtain 1:100 diluent.Take 1:100 diluent 1ml, be added to 100ml sterile physiological
In saline, fully mix 1:10000 with mycete diluent (this diluent every milliliter contain mold colony
Number substantially 100), standby.
Take 1:10000 band bacterium solution, draw 1ml in plate with 1ml sterilizing suction pipe.Weigh each antifungus agent 1g respectively
It is placed in the conical flask of 250ml sterilizing, adds the normal saline 100ml after sterilizing, place and vibrate on agitator
30min takes off, and puts and is heated up to about 50 DEG C 3min-5min on electric furnace, and the effective ingredient making antifungus agent is the most molten
Solve.Take with antifungus agent solution 1ml (fully taking again after mixing), be positioned over 1ml mycotic spore solution
Plate in, shake up.Each antifungus agent sample all makees double ware.By the high salt of cool to about 45 DEG C after sterilizing
Czapek's medium 15ml-20ml injects in each plate, fully mixes, is inverted in 25-30 DEG C after agar solidification
Incubator in, every day observe, record clump count, cultivate observe 5d.Each batch sample does two parts containing 1ml
Mycete is not added with the blank assay of antifungus agent.To have added the mold colony number of various antifungus agent plate and to be not added with mildew-resistant
The mold colony number of the agent plate containing 1ml mycete compares, and calculates the bacteriostasis rate of each process.
Bacteriostasis rate=process group mold colony number/blank group mold colony number × 100%, table 2 is respectively joined after cultivating for 5d
The bacteriostasis rate of ratio.
The bacteriostasis rate result of each proportioning after table 2,5d cultivation
The every column data of ※ has the representative of same letter tail tag and there was no significant difference, and then has significant difference without same letter.
From bacteriostasis rate result, proportioning 13,14,16 bacteriostasis rate is higher than other process groups, uses including comparison
External antifungus agent Hay Armer, and there was no significant difference from each other, using these three process groups as tentatively
The result of screening, and it is respectively designated as CA1, CA2, CA3, final the selection result and proportioning thereof are shown in Table 3.
Table 3, results of preliminary screening and proportioning thereof
Embodiment 2: the Baling at high moisture screening of additive formula
More excellent proportioning CA1 that Preliminary screening in embodiment 1 gone out, CA2, CA3 are respectively according to Hay quality
0.15%, 0.3%, 0.45% uniformly it is sprinkling upon the alfalfa hay that water content is about 28% and ties up；Hay Armer(HA)
Uniformly it is sprinkling upon alfalfa hay according to the 0.3% of Hay quality and ties up (all process group settings are shown in Table 4).Afterwards
Carry out bundling, non-additive high-moisture 27～28% water content (CKg) and low moisture 17% water content are set
(CKd) Radix Glycyrrhizae bundle is two comparisons.Each process 5 repetition, often bundle weighs about 20kg, totally 60 bundle.From
From bundling, every morning 10:00 thermocouple measures bale temperature, until all bale temperature become
Till stable, each process is surveyed 5 and is trussed hay, and often truss hay survey 3 times.
Table 4, process group set
Experimental result is as follows:
Result after table 5, each group storage 60 days
The every column data of ※ has the representative of same letter tail tag and there was no significant difference, and then has significant difference without same letter
Table 6, storage initial stage Radix Glycyrrhizae bundle variations in temperature (DEG C)
According to above test result analysis, comprehensive indices, show that the proportioning of this mildewproof additive is the Cortex Eucommiae
Extract 10-30 mass parts, garlicin 10-60 mass parts, Cortex Cinnamomi powder 10-80 mass parts, potassium sorbate
1.32-4 mass parts, sodium diacetate 5.28-8 mass parts, montmorillonite 2 6.4-40 mass parts.Suitably add model
Enclose the 0.3%-0.45% for Hay quality.This mildewproof additive is used to compare with the high-moisture without antifungus agent
Group is compared, and bale storage life temperature can be made to reduce, temperature peak reduces, Radix Glycyrrhizae mycete quantity, fiber, non-
Proteinic nitrogen reduces, and nutritional labeling and azelon digestibility increase.Each nutritional labeling compares also above low moisture
Group and state's external additive HA group.Wherein perfect additive processing mode be process 5, i.e. optimum formula be CA2
(Cortex Eucommiae extract 20%, garlicin 20%, Cortex Cinnamomi powder 27%, potassium sorbate 1.32%, sodium diacetate 5.28%,
Montmorillonite 2 6.4%), optimum addition is the 0.3% of high-moisture (water content 28%) Nutritive Value of Alfalfa Hay number,
Alfalfa hay nutritional labeling after preservation is higher, and CP17.83%, WSC22.32g/kg, fungi count 18.01 ×
103g-1DM, NPN 4.34g/kg, ash 13.01%, CP degradation rate 90.3%, NDF degradation rate 45.8%, ADF
Degradation rate 30.7%.The alfalfa hay composite mildewproof additive using effect that the present invention provides is ideal, and
Use cost is significantly less than the external import antifungus agent HAY FARMER of use, and domestic pasture can be afforded to use
Antifungus agent, can effectively solve the problem that pasture storage alfalfa hay is mouldy, reduce feedstuff loss for pasture, carry
Rise pasture farming benefit.
Above a kind of alfalfa hay composite mildewproof additive provided by the present invention is described in detail.This
Principle and the embodiment of the present invention are set forth by literary composition by detailed description of the invention, simply use described above
In helping to understand method and the core concept thereof of the present invention.It should be pointed out that, the common skill for the art
For art personnel, under the premise without departing from the principles of the invention, it is also possible to the present invention is carried out some improvement and
Modifying, these improve and modify in the protection domain also falling into the claims in the present invention.
Claims (8)
1. an alfalfa hay composite mildewproof additive, it is characterised in that by the component of following mass parts
Composition:
Alfalfa hay composite mildewproof additive the most according to claim 1, it is characterised in that by with
The component composition of lower mass parts:
Alfalfa hay composite mildewproof additive the most according to claim 1 or claim 2, it is characterised in that
Described Cortex Eucommiae extract is with the Cortex Eucommiae for extracting raw material, prepares with the mixed solution extraction of alcohol with water,
Chlorogenic acid content >=10%.
Alfalfa hay composite mildewproof additive the most according to claim 1 or claim 2, it is characterised in that
Described garlicin is artificial extraction or synthetic, is mainly composed of trithio allyl ethers chemical combination
Thing.
Alfalfa hay composite mildewproof additive the most according to claim 1 or claim 2, it is characterised in that
Described potassium sorbate is that content is more than >=98% food-class high-purity additive.
Alfalfa hay composite mildewproof additive the most according to claim 1 or claim 2, it is characterised in that
Described sodium diacetate is that content is more than >=98% food-class high-purity additive.
Alfalfa hay composite mildewproof additive the most according to claim 1 or claim 2, it is characterised in that
Described montmorillonite is sodium montmorillonite or ca-montmorillonite.
8. alfalfa hay composite mildewproof additive described in claim 1-7 is in alfalfa hay is preserved
Application, it is characterised in that: alfalfa hay moisture is 30% and less, alfalfa hay per ton
Uniformly add 3～4.5kg composite mildewproof additives.
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| CN201610364000.9A CN105941871A (en) | 2016-05-26 | 2016-05-26 | Compound mouldproof additive for alfalfa hay |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106615646A (en) * | 2016-11-15 | 2017-05-10 | 黄家七 | Compounding method of high-quality lucerne hay |
| CN106616203A (en) * | 2016-11-15 | 2017-05-10 | 黄家七 | Mildew-proof composition applied to storage of alfalfa hay and preparation method thereof |
| CN107319101A (en) * | 2017-06-15 | 2017-11-07 | 深圳市博裕家园生物科技有限公司 | Ferment nonreactive protein sources material and its preparation method and application |
| CN107969603A (en) * | 2017-12-29 | 2018-05-01 | 隆安县江泉牧业有限公司 | Nuisanceless type Midew preventive for feed and preparation method thereof |
| CN108125030A (en) * | 2017-12-29 | 2018-06-08 | 隆安县江泉牧业有限公司 | Mould-proof-type catalo feed and preparation method thereof |
| CN112401106A (en) * | 2020-10-10 | 2021-02-26 | 中国农业科学院草原研究所 | Mildew preventive suitable for storing high-moisture natural pasture and preparation method thereof |
| CN112401069A (en) * | 2020-10-10 | 2021-02-26 | 中国农业科学院草原研究所 | Safe mildew-proof process for natural forage grass |
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| CN102389033A (en) * | 2011-09-30 | 2012-03-28 | 广东紫金正天药业有限公司 | Composition containing montmorillonite, and application of composition |
| CN103859216A (en) * | 2014-03-13 | 2014-06-18 | 侯伟革 | Composite mildew preventive for animal feed and application of composite mildew preventive |
| CN105603726A (en) * | 2015-12-17 | 2016-05-25 | 黄南南 | Environmentally friendly additive for cotton and linen mildew proofing |
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| CN102389033A (en) * | 2011-09-30 | 2012-03-28 | 广东紫金正天药业有限公司 | Composition containing montmorillonite, and application of composition |
| CN103859216A (en) * | 2014-03-13 | 2014-06-18 | 侯伟革 | Composite mildew preventive for animal feed and application of composite mildew preventive |
| CN105603726A (en) * | 2015-12-17 | 2016-05-25 | 黄南南 | Environmentally friendly additive for cotton and linen mildew proofing |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106615646A (en) * | 2016-11-15 | 2017-05-10 | 黄家七 | Compounding method of high-quality lucerne hay |
| CN106616203A (en) * | 2016-11-15 | 2017-05-10 | 黄家七 | Mildew-proof composition applied to storage of alfalfa hay and preparation method thereof |
| CN107319101A (en) * | 2017-06-15 | 2017-11-07 | 深圳市博裕家园生物科技有限公司 | Ferment nonreactive protein sources material and its preparation method and application |
| CN107319101B (en) * | 2017-06-15 | 2021-01-01 | 深圳市博裕家园生物科技有限公司 | Fermented antibiotic-free protein source material and preparation method and application thereof |
| CN107969603A (en) * | 2017-12-29 | 2018-05-01 | 隆安县江泉牧业有限公司 | Nuisanceless type Midew preventive for feed and preparation method thereof |
| CN108125030A (en) * | 2017-12-29 | 2018-06-08 | 隆安县江泉牧业有限公司 | Mould-proof-type catalo feed and preparation method thereof |
| CN112401106A (en) * | 2020-10-10 | 2021-02-26 | 中国农业科学院草原研究所 | Mildew preventive suitable for storing high-moisture natural pasture and preparation method thereof |
| CN112401069A (en) * | 2020-10-10 | 2021-02-26 | 中国农业科学院草原研究所 | Safe mildew-proof process for natural forage grass |
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Application publication date: 20160921 |