CN105941479A - Fungus medicine combination containing phenyl amide bactericides, dithiocarbamate bactericides and biocontrol bacteria, preparation and application - Google Patents
Fungus medicine combination containing phenyl amide bactericides, dithiocarbamate bactericides and biocontrol bacteria, preparation and application Download PDFInfo
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- CN105941479A CN105941479A CN201610493033.3A CN201610493033A CN105941479A CN 105941479 A CN105941479 A CN 105941479A CN 201610493033 A CN201610493033 A CN 201610493033A CN 105941479 A CN105941479 A CN 105941479A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/74—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N43/76—1,3-Oxazoles; Hydrogenated 1,3-oxazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
- A01N47/14—Di-thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/26—Oxidation products of dithiocarbamic acid derivatives, e.g. thiuram sulfides
Abstract
The invention discloses a fungus medicine combination containing phenyl amide bactericides, dithiocarbamate bactericides and biocontrol bacteria, a preparation and application, and belongs to the field of plant protection and agricultural pharmacology. The fungus medicine combination is prepared from the phenyl amide bactericides, the dithiocarbamate bactericides and biocontrol bacteria powder according to the mass ratio of (1-10):(1-10):(1-10); the number of living bacteria in the bacteria powder is not less than 106 cfu/g. Preferably, the biocontrol bacteria are bacillus amyloliquefaciens ZY-9-13, with the preservation No. of CGMCC No. 6162. The phenyl amide bactericides can suppress the activity of RNA polymerase in pathogenic bacteria, and the dithiocarbamate bactericides is an inhibitor with multiple acting sites; the matched use of the two types of bactericides and the bacillus amyloliquefaciens can perfrom excellent prevention and treatment effect on tobacco root knot nematode and tobacco black shank.
Description
Technical field
The present invention relates to a kind of bacterium drug composition containing phenylamide, dithiocarbamate antibacterial and biocontrol microorganisms,
Also relate to comprise preparation and the application of this bacterium drug composition, belong to plant protection and Pesticide Science field.
Background technology
Black shank (Tobacco black shank) is a kind of worldwide crushing soil-borne disease, and its pathogen is epidemic disease
Mycete oronoco [Phytophthora parasitica var nicotianae (Breda de Hann) Tuker].Phytophthora nicotianae is at cigarette
The young plant phase all can infect to Adult plant, mainly infects tobacco stem stalk, it is possible to infect blade.Black shank forms water at stem foot
Stain shape black speck, cortical tissue's blackening depression, and extend up and down along stem, marrow pitchy is downright bad and drying shrinkage is video disc shape (ten thousand
Wei Xiao, He Xiaobing, Qu Pingzhi, etc. black shank early prevention technology [J]. plant doctor, 2014 (02): 50-50).Blade is caught an illness
Rear be just water soaking mode dirty-green stigma, after to expand as central authorities' yellowish-brown downright bad, there is the plaster shape black speck taking turns stricture of vagina at edge, and Herb withers
Listless (Sun Jiping, Li Xuejun, Wu Zhaohui, etc. the progress [J] of black shank. hubei agricultural science, 2011,
50(16):3253-3256).Phytophthora nicotianae is survived the winter in soil or compost with diseased plant residuum with akinete with mycelium, becomes
The primary source of infection of next year.The Sporangium produced on diseased plant or the zoospore discharged are carried out by ground flowing water or with wind and rain
Infect again (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. agriculture in south
Report, 2003 (2): 42-43).Along with the continuous expansion of the later stage eighties 20th century China's tobacco planting area, black shank
Extension rapidly.Black shank average attack rate is 10%～20%, and the field that sickness rate is high reaches 75%, and serious even makes
Become to have no harvest (Chen Ruitai, Zhu Xianchao, Wang Zhifa, etc. 16, whole nation main product cigarettes save (district) Nicotiana tabacum L. infectious disease investigation report [J]. China
Nicotiana tabacum L. science, 1997 (04): 1-7).
Chemical prevention is the important measures of black shank prevention and control, uses during wherein the pesticide such as metalaxyl, Mancozeb is production
Relatively broad medicament.Metalaxyl is phenylamide systemic fungicide, and the mid-80 is at China's production and application, at ovum
Playing an important role in fungus diseases preventing and treating, its mechanism of action is the polymerase activity of suppression pathogen, and RNA interfering synthesizes.
But be as in producing is commonly used, and the resistance problem of chemical bactericide occurs the most therewith.The research such as Wang Wanneng shows, changes
After learning chemicals treatment, the mycelia physical ability of black shank causes the change of mating type, and then the mutant strain that develops immunity to drugs, even
The Drug resistance of some bacterial strain can stablize heredity, wherein the most prominent with metalaxyl for representing phenyl amide series bactericidal agent, and
Resistance level the highest (Wang Wanneng, Xiao Chonggang. the integrated control of black shank and progress [J] thereof. Guangxi Agricultural section
Learn, 2003, (2): 42-44).The field in the field that Shew reports plantation Nicotiana tabacum L. uses metalaxyl, balck shank colony for successive years
The sensitivity of metalaxyl is declined, average EC50Value increases (Shew H D.Response of Phytophthora year by year
parasitica var.nicotianae to metalaxy exposure[J].Plant Disease,1985,69:559-562).And chemical agent
The compounding consumption that can reduce a kind of microbiocidal composition, delays drug-fast generation, reduces production cost simultaneously, be phytopathy
One of important measures in evil preventing and treating.
The chemical prevention of black shank is the important measures in comprehensive control of disease, and its compound drug also has relevant report at present
And patent.As disclosed in the patent of invention of publication No. CN105265448A a kind of for preventing and treating Nicotiana tabacum L. root-rot, the killing of balck shank
Microbial inoculum, is mainly made up of metalaxyl and hymexazol, is watered and uses after being aided with methanol, emulsifying agent；Publication No.
A kind of bactericidal composition disclosed in the patent of invention of CN104430438A, its effective ingredient is Famoxate and cymoxanil, two
The weight ratio of person is 80:1～1:80, it is adaptable to the plant disease that preventing and treating is caused by pathogenic fungi, especially black shank and melon
Downy mildew on the crops such as fruits and vegetables, epidemic disease etc.；The patent of invention of publication No. CN103478163A discloses a kind of containing benzene
The bactericidal pesticide composition of metsulfovax, also includes AL-PHOSETHYL aluminum tris(ethyl phosphite) in addition to benzene metsulfovax, and the weight ratio of the two is 1:40～60:1, this group
Compound can be used for preventing and treating black shank, crop epidemic disease and downy mildew etc..
The biological living that biocontrol bacterial strain is naturally-occurring, has good Environmental compatibility.Utilize biocontrol bacterial strain preventing and treating Nicotiana tabacum L. black
Shin disease has that preventive effect is high, safety is good, low cost and other advantages.Disclosed in the patent of invention of notification number CN101220335B one
The bacterial strain of kind preventing and treating black shank, entitled Paecilomyces lilacinus ZY-19-2, deposit number CCTCC NO:M207196,
This bacterial strain has stronger inhibitory action to tobacco black shank bacterium, can surely grow the dip-dye tobacco plant rhizosphere opposing pathogenic bacteria；Bulletin
The patent of invention of number CN103614319B also discloses that a kind of A Shi bacillus cereus J-6 for preventing and treating black shank, protects
Hiding numbering CCTCC NO:M2013475, this bacterial strain can be induced Nicotiana tabacum L. antagonism balck shank thus be played phytophthora prevention effect；Public
A kind of resisting meloidogyne and tobacco phytophthora nicotianae endophyte of holding concurrently, title disclosed in the patent of invention of announcement CN102925393B
For bacillus amyloliquefaciens ZY-9-13, deposit number CGMCC NO:6162, this bacterial strain is to root knot nematode in tobacco and black shin
Disease is respectively provided with prevention effect, can be used in mixed way with tobacco seedling medium or apply around rhizosphere when tobacco plant is transplanted, biological and ecological methods to prevent plant disease, pests, and erosion
Effect is notable.But, above-mentioned patent is all not directed to content associated with antibacterial and biocontrol agent.
The agricultural production system of sustainable development requires to reduce the input of pesticide, and controlling disease biological, chemical cooperated can alleviate
Pressure to environment, delays chemical agent is produced resistance, make drug effect more stable and lasting (Yin Jingfang. biological-chemical is collaborative
Preventing and treating capsicum epidemic disease bacteria medicament mixture preliminary study [D]. China Agricultural University, 2006).There are some researches show, biocontrol agent and change
Learn composite reagent and multiple pathogen is all had good prevention effect.As Tarlaromyces flavus and first frost MnZn are compounding, can to prevent and treat Semen Tritici aestivi red
Mildew (Mao Xueqin, Peng Zhirong, Qiu Haiping, etc. wheat scab is prevented by biocontrol bacterial strain MT-06 fermentation condition and composite bactericide
Effect [J]. Zhejiang Agriculture science, 2013 (07): 821-824), Bacillus subtillis and Tebuconazole compound that can to prevent and treat faba bean Fusarium wilt (old
Will friendship, Haiying REN, Liu Yongfeng, etc. Tebuconazole and bacillus subtilis synergism preventing and treating faba bean Fusarium wilt and Synergistic Mechanisms pre-test
[J]. Pesticide Science journal, 2002 (4): 40-44), Bacillus subtillis is compounding with Difenoconazole, Fluoxastrobin etc. can prevent and treat Fructus Mali pumilae wheel
Stricture of vagina disease (week is cold. and antibacterial and biocontrol microorganisms are coordinated to control Fructus Mali pumilae and are adopted rear ring spot and mechanism of action [D]. Agricultural University Of Anhui, 2009),
Jingganmycin and Bacillus subtillis be collaborative prevent and treat rice sheath blight disease (Chen Zhiyi, Liu Yongfeng, Lu Fan. jingganmycin and biocontrol microorganisms
Bs-916 works in coordination with disease-control effect and Synergistic Mechanisms [J]. plant protection journal, and 2003,30 (4): 429-434.) etc., but above bacterium
The preventing and treating target that recurrence due to taking drug is joined all is not related to Phytophthora nicotianae.The patent of invention of publication No. CN104381303A disclose a kind of for
The bacterium drug composition of preventing and treating fungal disease, by riboflavin with bacillus subtilis Tpb55 mycopowder according to mass ratio
1.21～2.34:97.96～98.79 compounding form, and compare and bacillus subtilis Tpb55 mycopowder is administered alone, this bacterium drug composition
The disease control effect such as black shank, rust can be significantly improved, strengthen plant disease resistance, but also only reach and chemistry
The prevention effect that medicament is suitable.
Summary of the invention
It is an object of the invention to provide a kind of bacterium medicine containing phenylamide, dithiocarbamate antibacterial and biocontrol microorganisms
Compositions, said composition has the prevention effect of excellence to tobacco root-knot, black shank etc., and preventive effect is significantly better than
Use the antibacterial of single kind, biocontrol microorganisms microbial inoculum and any of the above-described kind of series bactericidal agent and the combination of biocontrol microorganisms microbial inoculum, and can
Delay chemical drug-fast generation.
Meanwhile, the present invention also provides for a kind of bacterium medicine preparation comprising above-mentioned bacterium drug composition.
Finally, the present invention reoffers a kind of above-mentioned bacterium drug composition, bacterium medicine preparation preventing and treating tobacco root-knot and/or Nicotiana tabacum L.
Application in balck shank.
In order to realize object above, the technical solution adopted in the present invention is:
Bacterium drug composition, is made up of phenyl amide series bactericidal agent, dithiocarbamate antibacterial and biocontrol microorganisms mycopowder,
By quality ratio, phenyl amide series bactericidal agent: dithiocarbamate antibacterial: biocontrol microorganisms mycopowder=1～10:1～10:1～10,
In mycopowder, viable count is not less than 106cfu/g。
Described phenyl amide series bactericidal agent (PAFs) includes that acylalaninies series bactericidal agent is (such as metalaxyl, Metalaxyl-M, benzene
Frost M 9834 clever, smart, furalaxyl), butyrolactone series bactericidal agent (such as fenfuram) and oxazolidone series bactericidal agent (as
Wakil) (Wang Hancheng, Li Wenhong, Feng Yonggang, etc. the history of black shank chemical prevention and present situation [J]. Chinese tobacco
Report, 2011,17 (5): 96-102).Specifically it is selected from metalaxyl, Metalaxyl-M, M 9834, essence M 9834, furalaxyl, first furan
One or more in amide, Wakil etc..As individually selected metalaxyl, M 9834 or furalaxyl, or select essence first frost
Spirit and essence M 9834, metalaxyl and fenfuram, the combination of furalaxyl and Wakil, also or select M 9834, furalaxyl
Combination with Wakil.Compare the single a kind of phenyl amide series bactericidal agent of use, bactericide compounded (two kinds and above antibacterial
With the use of) there is summation action or potentiation.Further, in composite bactericide, each component proportion can arbitrarily be chosen, as
M 9834 compounds according to mass ratio 1～10:1～10 with Wakil, and M 9834 and furalaxyl, fenfuram are according to mass ratio
1～10:1～10:1～10 compound, etc..Owing to combining form is various, do not enumerate.
Described dithiocarbamate antibacterial selected from zineb, ambam, maneb, Carbatene, Mancozeb,
One or more in propineb, Propineb, thiram, ferric dimethyldithiocarbamate, ziram etc..As individually select zineb,
Mancozeb, Propineb or thiram, or select the combination of maneb and Propineb, propineb and thiram, also
Or select the combination of zineb, Propineb and ziram.Compare the single a kind of dithiocarbamate antibacterial of use,
Bactericide compounded (two kinds and above antibacterial with the use of) have summation action or potentiation.Further, composite bactericide
Prevention effect to tobacco root-knot, black shank etc. is used in combination with biocontrol microorganisms to be better than being used alone chemical bactericide
Or biocontrol microorganisms microbial inoculum.Meanwhile, in composite bactericide, each component proportion can arbitrarily be chosen, as Mancozeb and Propineb according to
Mass ratio 1～10:1～10 compounds, and and for example zineb is multiple according to mass ratio 1～10:1～10:1～10 with propineb, ziram
Join, etc..Owing to combining form is various, do not enumerate.
Preferably, in bacterium drug composition phenyl amide series bactericidal agent selected from metalaxyl, Metalaxyl-M, M 9834, essence M 9834,
Any one in furalaxyl, fenfuram, Wakil etc., dithiocarbamate antibacterial is selected from zineb, generation
In gloomy ammonium, maneb, Carbatene, Mancozeb, propineb, Propineb, thiram, ferric dimethyldithiocarbamate, ziram etc.
Any one.In phenyl amide series bactericidal agent, the mechanism of action of metalaxyl, furalaxyl, fenfuram etc. is same or similar,
In like manner dithiocarbamate antibacterial, therefore chooses any one kind of them compounding from above-mentioned two series bactericidal agents respectively, compound sterilizing
Agent is used in combination with biocontrol microorganisms again, can not only simplify formula, also can reach tobacco root-knot, black shank etc. simultaneously
To good prevention effect.The metalaxyl of phenylamide, M 9834, Wakil etc. are that a class has protection and therapeutical effect
Systemic fungicide, can be absorbed rapidly by plant root, leaf, the tip, and by non-lifes such as fascicular conduit and intercellular substances
Space system of living shifts to plant top, has the highest activity to phytophthora, can be used for stem, leaf, seed and soil treatment.
Dithiocarbamate antibacterial is multiaction site inhibitor, and is not likely to produce resistance.
Described biocontrol microorganisms mycopowder can refer to method in patent (notification number CN102925393B) to be prepared.Biocontrol microorganisms is preferably and solves
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) ZY-9-13, deposit number: CGMCC No.6162, preservation
Time: on 05 29th, 2012, depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center,
Unit address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.This bacterial strain is held concurrently anti-root knot nematode in tobacco and Phytophthora nicotianae, especially
It can suppress tobacco black shank bacterium mycelial growth and spore germination, the most surely grows in tobacco rhizosphere.See patent " a kind of double
Resisting meloidogyne and tobacco phytophthora nicotianae endophyte and application thereof " (notification number: CN102925393B).Experiment proves that,
Phenyl amide series bactericidal agent, dithiocarbamate antibacterial are to bacillus amyloliquefaciens (CGMCC No.6162)
Growth effect is faint, and the compatibility between antibacterial and bacterial strain is good, can carry out bacterium medicine combination.
Described bacterium drug composition can use conventional method to be prepared as any one dosage form being suitable to agricultural use, such as wettable powder
Agent, suspending agent, water dispersion granule, cream, aqueous emulsion, microemulsion, water preparation, microcapsule, soluble powder etc..
In preparation, bacterium drug composition is effective ingredient, and its content is the 0.1～99.9% of preparation gross mass.
Bacterium medicine preparation, comprises above-mentioned bacterium drug composition, and acceptable auxiliary agent in one or more Pesticide Sciences.Described auxiliary agent
Including wetting dispersing agent, stabilizer, antifreezing agent, disintegrating agent, thickening agent, defoamer, organic solvent, carrier etc., and
Other are of value to effective ingredient and the most stably play the material of drug effect, are to commonly use in pesticidal preparations or allow each of use
Plant composition.Specifically chosen and the consumption of auxiliary agent can be determined by routine test according to recipe requirements.
Described wetting dispersing agent be selected from sodium lauryl sulphate, dodecyl sodium sulfate, dodecylbenzene sodium sulfonate, 12
Alkyl benzene calcium sulfonate, calcium lignosulfonate, sodium lignin sulfonate, lauric acid sodium sulfate, alkyl polyoxyethylene ether sulfonate,
Alkylphenol polyoxyethylene formaldehyde condensation products, polyoxyethylene polyoxypropylene block copolymer, polyoxyethylene lauryl ether sodium sulfate,
Alkyl naphthalene formaldehyde condensate sulfonates, pull open in powder, alkylpolyoxyethylene phosphate ester, hydroxymethyl cellulose etc. appoint
Anticipate one or more.
Described stabilizer be selected from pyrogallol, BHT, BHA, citric acid, epoxy soybean oil, oleum lini, eugenol,
Oleum Ricini, olive oil, hydroquinone, salicylic acid, sodium sorbate, butyl glycidyl ether, phenyl glycidyl ether, first
Any one or more in phenyl glycidyl ether, polyethylene glycol diglycidyl ether, bisnaphthol, a naphthols.
Described antifreezing agent is selected from any one in glycerol, propylene glycol, ethylene glycol, carbamide, sodium chloride.
Described disintegrating agent is selected from ammonium sulfate, calcium chloride, magnesium chloride, aluminum chloride, bentonite, phosphoric acid dihydro amine, carbamide etc.
In any one or more.
Described thickening agent is selected from xanthan gum, arabic gum, polyvinyl alcohol, carboxymethyl cellulose, methylcellulose, miscellaneous many
Sugar, soluble starch, sucrose, glucose, sodium alginate, acrylic acid series polymeric compounds, polyvinylpyrrolidone, Colophonium,
Any one or more in Gypsum Fibrosum, sodium sulfate, osseocolla, soybean protein, dextrin, silicic acid, Magnesiumaluminumsilicate etc..
Described defoamer is selected from silicone oil defoamer, silicone defoamer, C10～20Saturated fat acids defoamer, C8～10
Any one in aliphatic alcohols defoamer.
Described organic solvent is selected from toluene, dimethylbenzene, alkylbenzene, isopropanol, butanol, ethylene glycol, diethylene glycol, three sweet
Alcohol, propylene glycol, glycerol, sorbitol, benzyl alcohol, Hexalin, acetone, methyl iso-butyl ketone (MIBK), Ketohexamethylene, dimethyl
Any one or more in Methanamide, ketopyrrolidine, dichloromethane, chloroform, carbon tetrachloride, vegetable oil etc..
Described carrier is selected from quartz, clay, montmorillonite, silicon dioxide, kieselguhr, Pumex, Gypsum Fibrosum, Talcum, swelling
Any one in soil, Kaolin, potter's clay, white carbon, calcite, marble, meerschaum, dolomite, attapulgite etc.
Plant or multiple.
The application of bacterium drug composition, specially bacterium drug composition answering in preventing and treating root knot nematode in tobacco and/or black shank
With.
The application of bacterium medicine preparation, the specially bacterium medicine preparation application in preventing and treating root knot nematode in tobacco and/or black shank.
Beneficial effects of the present invention:
In the present invention, bacterium drug composition is conciliate starch spore by phenyl amide series bactericidal agent, dithiocarbamate antibacterial
Bacillus (CGMCC No.6162) mycopowder forms, and wherein phenyl amide series bactericidal agent can suppress RNA polymerase in pathogen
Activity, dithiocarbamate antibacterial is multiaction site inhibitor, and is not likely to produce resistance, this two class sterilize
Agent has excellent prevention effect, preventive effect with bacillus amyloliquefaciens with the use of to tobacco root-knot, black shank
It is significantly better than and uses the antibacterial of single kind, biocontrol microorganisms microbial inoculum and any of the above-described kind of series bactericidal agent and the group of biocontrol microorganisms microbial inoculum
Close, and chemical drug-fast generation can be delayed, be particularly well-suited to the preventing and treating of field tobacco balck shank.
Detailed description of the invention
The present invention is only described in further detail by following embodiment, but does not constitute any limitation of the invention.
Embodiment 1
Bacterium drug composition, is made up of M 9834, zineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with
Mass ratio meter, M 9834: zineb: bacillus amyloliquefaciens mycopowder=1:1:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 2
Bacterium drug composition, is made up of Metalaxyl-M, zineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, Metalaxyl-M: zineb: bacillus amyloliquefaciens mycopowder=3:2:10, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 3
Bacterium drug composition, is made up of metalaxyl, propineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, metalaxyl: propineb: bacillus amyloliquefaciens mycopowder=10:5:2, in mycopowder, viable count is not less than 106
cfu/g。
Embodiment 4
Bacterium drug composition, is made up of Wakil, Propineb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with
Mass ratio meter, Wakil: Propineb: bacillus amyloliquefaciens mycopowder=10:10:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 5
Bacterium drug composition, is made up of fenfuram, Mancozeb and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, fenfuram: Mancozeb: bacillus amyloliquefaciens mycopowder=10:1:10, in mycopowder, viable count is not less than 106
cfu/g。
Embodiment 6
Bacterium drug composition, is made up of furalaxyl, thiram and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder, with
Mass ratio meter, furalaxyl: thiram: bacillus amyloliquefaciens mycopowder=3:10:9, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 7
Bacterium drug composition, is made up of essence M 9834, ziram and bacillus amyloliquefaciens (CGMCC No.6162) mycopowder,
By quality ratio, essence M 9834: ziram: bacillus amyloliquefaciens mycopowder=5:5:1, in mycopowder, viable count is not less than 106cfu/g。
Embodiment 8
Bacterium drug composition, by metalaxyl, fenfuram, Mancozeb and bacillus amyloliquefaciens (CGMCC No.6162)
Mycopowder forms, by quality ratio, and metalaxyl: fenfuram: Mancozeb: bacillus amyloliquefaciens mycopowder=3:5:6:10, mycopowder
Middle viable count is not less than 106cfu/g。
Embodiment 9
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Wakil 26 parts, thiram 33 parts, solves starch spore bar
(in mycopowder, viable count is not less than 10 to bacterium (CGMCC No.6162) mycopowder 5 parts6Cfu/g), dodecyl sodium sulfate 7 parts,
3 parts of Laurel alcohol radical sodium sulfate, sodium lauryl sulphate 7 parts, ethyl cellulose 3 parts, sucrose 5 parts, 15 parts of kieselguhr,
Attapulgite 12 parts；Preparation method is: accurately takes each raw material, grinding and sieving, mix homogeneously according to mass fraction, obtains
To wettable powder.
Embodiment 10
Bacterium medicine preparation, is made up of the raw material of following mass fraction: metalaxyl 7 parts, Mancozeb 6 parts, solves starch spore bar
(in mycopowder, viable count is not less than 10 to bacterium (CGMCC No.6162) mycopowder 43 parts6Cfu/g), calcium lignosulfonate 12 parts,
Sodium lauryl sulphate 5 parts, sucrose 4 parts, white carbon 12 parts, 15 parts of kieselguhr；Preparation method is with embodiment 9.
Embodiment 11
Bacterium medicine preparation, is made up of the raw material of following mass fraction: essence M 9834 20 parts, propineb 25 parts, solves starch
(in mycopowder, viable count is not less than 10 to bacillus cereus (CGMCC No.6162) mycopowder 20 parts6Cfu/g), dodecyl sodium sulfonate
12 parts of sodium, 8 parts of Laurel alcohol radical sodium sulfate, calcium lignosulfonate 5 parts, ethyl cellulose 8 parts, sucrose 5 parts, kaolinite
15 parts of soil；Preparation method is with embodiment 9.
Embodiment 12
Bacterium medicine preparation, is made up of the raw material of following mass fraction: furalaxyl 16 parts, Mancozeb 8 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 30 parts6Cfu/g), dodecyl sodium naphthalene sulfonate
6 parts, pull open 5 parts of powder, 2 parts of sodium sulfate, xanthan gum 8 parts, sucrose 4 parts, Kaolin 12 parts；Preparation method is: press
Accurately take each raw material, pelletize after mix homogeneously according to mass fraction, be dried, sieve, obtain suspending agent.
Embodiment 13
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Metalaxyl-M 33 parts, Propineb 15 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 8 parts6Cfu/g), dodecyl sodium naphthalene sulfonate
12 parts, pull open 3 parts of powder, starch 5 parts, 2 parts of sodium sulfate, xanthan gum 5 parts, sucrose 5 parts, bentonite 12 parts, silicon
Diatomaceous earth 18 parts；Preparation method is with embodiment 12.
Embodiment 14
Bacterium medicine preparation, is made up of the raw material of following mass fraction: M 9834 20 parts, ziram 20 parts, solves starch spore bar
(in mycopowder, viable count is not less than 10 to bacterium (CGMCC No.6162) mycopowder 35 parts6Cfu/g), dodecyl sodium naphthalene sulfonate 6
Part, pull open 8 parts of powder, 3 parts of sodium sulfate, sucrose 5 parts, bentonite 12 parts, 16 parts of kieselguhr；Preparation method is with implementing
Example 12.
Embodiment 15
Bacterium medicine preparation, is made up of the raw material of following mass fraction: fenfuram 10 parts, zineb 19 parts, solves starch spore
(in mycopowder, viable count is not less than 10 to bacillus (CGMCC No.6162) mycopowder 19 parts6Cfu/g), polyvinylpyrrolidone 6
Part, dodecyl phenol polyethenoxy ether 3 parts, dodecylbenzene sodium sulfonate 5 parts, 4 parts of oxirane, ethyl cellulose 12
Part, attapulgite 15 parts；Preparation method is: accurately take each raw material according to mass fraction, ultra micro air-flow powder after mix homogeneously
Broken, pelletize, obtain water dispersion granule.
Embodiment 16
Bacterium medicine preparation, is made up of the raw material of following mass fraction: M 9834 8 parts, Wakil 12 parts, propineb 15
Part, ziram 10 parts, (in mycopowder, viable count is not less than bacillus amyloliquefaciens (CGMCC No.6162) mycopowder 15 parts
106Cfu/g), sodium lignin sulfonate 7 parts, nekal 3 parts, dodecylbenzene sodium sulfonate 5 parts, polyethylene pyrrole
Pyrrolidone 5 parts, starch 5 parts, sucrose 3 parts, 18 parts of kieselguhr；Preparation method is with embodiment 15.
In other embodiments of the invention, also bacterium medicine preparation can be made the dosage forms such as aqueous emulsion, water preparation, microcapsule, help
Agent uses this area conven-tional adjuvants.
In other embodiments of the invention, also can be from phenyl amide series bactericidal agent, dithiocarbamate antibacterial
Optional multiple compounding, composite bactericide combines with biocontrol microorganisms or makes bacterium medicine preparation.
Comparative example 1
Bacterium medicine preparation, is made up of the raw material of following mass fraction: Wakil 59 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), other auxiliary agents and preparation method are with embodiment 9.
Comparative example 2
Bacterium medicine preparation, is made up of the raw material of following mass fraction: thiram 59 parts, bacillus amyloliquefaciens (CGMCC
No.6162) (in mycopowder, viable count is not less than 10 to mycopowder 5 parts6Cfu/g), other auxiliary agents and preparation method are with embodiment 9.
Bacillus amyloliquefaciens (CGMCC No.6162) mycopowder in the various embodiments described above and comparative example is with reference to patent (bulletin
Number CN102925393B) method is prepared as wettable powder in detailed description of the invention.
Test example
1, the synergistic function of bacterium drug composition measures
Mycelial growth rate method is used to measure tobacco black shank bacterium to antibacterial, bacillus amyloliquefaciens and the medicine of bacterium drug composition
Agent sensitivity.In table 1, bactericide original drug (not adding bacillus amyloliquefaciens) is 0.80 μ g/mL for examination concentration, in table 2
Phenylamide bactericide original drug (not adding bacillus amyloliquefaciens) is 0.05 μ g/mL for examination concentration, dithio in table 3
The former medicine of carbamate fungicide (not adding bacillus amyloliquefaciens) is 10.00 μ g/mL for examination concentration；Solve starch spore
The concentration of bacillus (CGMCC No.6162) bacteria suspension is 1010Cfu/mL, (all kinds of bactericide original drugs are corresponding for bacterium drug composition
Concentration+bacillus amyloliquefaciens (CGMCC No.6162) 1010Cfu/mL), compounding form is specifically shown in table 1 below～3.By diameter
5mm bacterium dish is placed in the oat medium central authorities of pastille, and every chemicals treatment is repeated 3 times, and to be processed as comparison without medicine, uses
Decussation method measures colony diameter, calculates the different disposal suppression ratio to tobacco black shank bacterium, evaluates bacterium drug composition to black
The inhibitory action of shin pathogenic bacteria, result see table 1～3.
Abbott mathematical model is used to calculate bacterium drug composition inhibition (Colby S, Calculating to pathogen
synergistic and antagonistic responses of herbicide combinations[J].Weeds,1967)。
Medicament is as follows to the theoretical suppression ratio computing formula of tobacco black shank bacterium:
E=(X1+X2+…)-X1·X2·…/100；
In formula: E represents medicament expection (theoretical) suppression ratio to tobacco black shank bacterium, X1、X2... represent respectively medicament A,
Medicament B ... the suppression ratio to tobacco black shank bacterium.
Evaluation methodology is: expectation (theoretical) suppression ratio is compared with practical measurement suppression ratio, when theoretical value ＜ actual value,
Show as potentiation；When theoretical value=actual value, show as summation action；When theoretical value ＞ actual value, show as
Antagonism.
Table 1 phenylamide, dithiocarbamate antibacterial and the bacillus amyloliquefaciens inhibition to mycelial growth
Table 2 phenyl amide series bactericidal agent and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
Table 3 dithiocarbamate antibacterial and the bacillus amyloliquefaciens inhibition to tobacco black shank bacterium mycelial growth
From table 1～3 it can be seen that conciliate starch spore containing phenyl amide series bactericidal agent, dithiocarbamate antibacterial
The bacterium drug composition of bacillus (CGMCC No.6162) is significantly better than use single kind to the inhibition of tobacco black shank bacterium
The combination of antibacterial, bacillus amyloliquefaciens and any of the above-described kind of series bactericidal agent and bacillus amyloliquefaciens.Show this
Between the bright middle each component of bacterium drug composition, there is synergistic function.
2, the bacterium medicine preparation greenhouse test of pesticide effectiveness to tobacco black shank bacterium
Inoculate tobacco black shank bacterium by greenhouse, and spray embodiment 9～16 and the bacterium medicine preparation of comparative example 1～2 preparation, tool
Body method is as follows: the method with reference to Dong Yu prepares bacterium paddy inoculum, and the bacterium cake of 20 diameter 5mm is added 500g sterilizing
Millet in, 28 DEG C cultivate 14d, standby after mix homogeneously (Dong Yu. preventing and treating black shank spacetabs type antibacterial research [D].
The Chinese Academy of Agricultural Sciences, 2015)；Zhongyan-100 tobacco seedlings is planted in the nutritive cube of 10 × 10cm, Nutrition Soil and the ratio of Vermiculitum
Example is 1:1, when tobacco seedlings length to 6～7 slice true leaf, carries out medicament root irrigation, and each nutritive cube adds medicinal liquid 50mL, each
Process 5 strain tobacco seedlings, not apply medicament, to add the process of 50mL clear water as comparison.Test sets three repetitions, dispenser 3d
After in cigarette strain root inoculate bacterium paddy 10g/ strain, carry out disease survey after dispenser 7d, according to below equation calculate prevention effect:
Prevention effect (%)=(comparison disease index-process disease index)/comparison disease index × 100%.
Black shank grade scale is with reference to People's Republic of China's tobacco business standard (YC/T39-1996), and result of the test is shown in
Table 4 below.In table 4,72% first frost mancozeb wettable powder is purchased from Zhejiang Heben Science & Technology Co., Ltd., 68% essence first frost MnZn
Water dispersible granules is purchased from Xian Zhengda crop protection company limited, and 50% thiram wettable powder is limited purchased from Shanxi section power science and technology
Company, bacillus amyloliquefaciens (CGMCC No.6162) microbial inoculum is specifically real with reference to patent (notification number CN102925393B)
Prepared by method in mode of executing, be more than matched group.
The table 4 bacterium medicine preparation prevention effect to black shank
From table 4, it can be seen that bacterium medicine preparation is respectively provided with the prevention effect of excellence to black shank in embodiment 9～16, anti-
Effect reaches more than 82%, is better than bacterium medicine preparation in commercially available chemical agent, bacillus amyloliquefaciens microbial inoculum and comparative example 1～2, for
The preventing and treating of balck shank provides novel alternate medications, by using with commercially available medicament rotation, it is possible to decrease drug-fast generation.
Claims (9)
1. bacterium drug composition, it is characterised in that: by phenyl amide series bactericidal agent, dithiocarbamate antibacterial and
Biocontrol microorganisms mycopowder forms, by quality ratio, and phenyl amide series bactericidal agent: dithiocarbamate antibacterial: biocontrol microorganisms bacterium
Powder=1～10:1～10:1～10, in mycopowder, viable count is not less than 106cfu/g。
Bacterium drug composition the most according to claim 1, it is characterised in that: described phenyl amide series bactericidal agent includes acyl
Base alanine series bactericidal agent, butanoic acid lactone series bactericidal agent, oxazolidone series bactericidal agent.
Bacterium drug composition the most according to claim 2, it is characterised in that: described phenyl amide series bactericidal agent is selected from first
One or more in frost spirit, Metalaxyl-M, M 9834, essence M 9834, furalaxyl, fenfuram, Wakil.
Bacterium drug composition the most according to claim 1, it is characterised in that: described dithiocarbamate sterilizes
Agent is selected from zineb, ambam, maneb, Carbatene, Mancozeb, propineb, Propineb, thiram, good fortune
One or more in U.S. ferrum, ziram.
5. according to the bacterium drug composition according to any one of Claims 1 to 4, it is characterised in that: described biocontrol microorganisms is Xie Dian
Afnyloliquefaciens ZY-9-13, deposit number: CGMCC No.6162.
6. comprise the bacterium medicine preparation of bacterium drug composition as according to any one of Claims 1 to 5.
Bacterium medicine preparation the most according to claim 6, it is characterised in that: also comprise and can connect in one or more Pesticide Sciences
The auxiliary agent being subject to.
8. as according to any one of Claims 1 to 5 bacterium drug composition preventing and treating root knot nematode in tobacco and/or black shank in
Application.
The application in preventing and treating root knot nematode in tobacco and/or black shank of the bacterium medicine preparation the most as claimed in claims 6 or 7.
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