CN1059260A - feed complex enzyme-protein production method - Google Patents
feed complex enzyme-protein production method Download PDFInfo
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- CN1059260A CN1059260A CN91105141A CN91105141A CN1059260A CN 1059260 A CN1059260 A CN 1059260A CN 91105141 A CN91105141 A CN 91105141A CN 91105141 A CN91105141 A CN 91105141A CN 1059260 A CN1059260 A CN 1059260A
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- production method
- aspergillus
- corn
- complex enzyme
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Fodder In General (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention relates to a kind of production method of feed complex enzyme-albumen.Be mix, add the water mixing after various raw materials are pulverized by beater grinder in proportion, through sterilization, cooling, insert that the solid microbe mixed culture ferments, after the fermentation ends by low temperature drying, get product after pulverizing.Product of the present invention has required active complex enzyme of animal and growth factor, and protein net added value height, feed nutrition are abundant, balanced, price of deed height, and can promote to digest.It is the processing of other agricultural basic processing industry waste residue of raw material that the present invention is equally applicable to the corn, the waste residue that produces in producing as corn alcohol, corn high fructose syrup.
Description
The present invention relates to a kind of multiple byproduct that produces in the cornstarch production process and production method of byproduct production feed complex enzyme one albumen in the plant process utilized.
At present, the protein shortage is a key factor of puzzlement feedstuff industry development.How existing protein resource being carried out degree of depth utilization, improve the price of deed, is an important topic of feedstuff industry.Around this content, the parties concerned has carried out very big effort, and power is sought on the way by way of solving this difficult problem.The method of having used at present has: (1) chemical method; Be hydrolyzed to handle as the raw material that feather, human hair etc. is contained keratoprotein and obtain protein hydrolysate and amino acid.This method investment is big, cost is higher.(2) physics method; Expanded as some feedstuff is pressurizeed, the internal structure of change raw material makes it to be easy to be assimilated by poultry.Because this method only changes raw material mix, composition is constant, so the price of deed is not too high.(3) bioanalysis; Be by raw material being carried out biochemical treatment, the structure of raw material and composition aspect being got a new look.This method cycle is long, the cost height.
How improve in the research of the price of deed, adopt plus enzyme abroad, be about to the finished product enzyme and add in the feed, obtain good effect, utilize plus enzyme to improve the utilization ratio of cereal in monogastric animal feeds such as pig, chicken as Canada.The U.S. adds enzyme preparation to contain feeds the chicken weightening finish obviously in the higher barley diet of crude fibre, and can reduce food consumption.The result of study of the Soviet Union has also confirmed above-mentioned conclusion.Domestic had Preliminary study to the Application and Development of feed enzyme preparation in recent years, obtained some progress, as Xiangfan City, Hubei biochemical research complex enzyme formulation added in the feed, obtains significantly result.Said method just directly adds the finished product enzyme in the feed simply, and various raw materials in the feed itself be there is no more influences.The main cause that influences the plant protein fodder remuneration in animal husbandry is the unbalanced of the contained nutrition component of certain plants albumen, and lack the unknown factor of the growth of animal that had in animal protein and the single cell protein, in corn protein, tryptophan and lysine content deficiency; The dregs of beans methionine content is less, and carotene and vitamin D content are less.
The purpose of this invention is to provide one and utilize the multiple accessory substance that produces in the cornstarch production, the leftover bits and pieces that the vegetable oil process produces is a primary raw material, by the fermentation of solid microbe mixed culture, produce the feed protein processing method of providing needed active complex enzyme composition in the animal metabolism process.
Purpose of the present invention is realized by following technical proposal.
With various raw materials, as corn lees, corn germ cake, corn protein powder, dregs of beans, mix, add the water mixing after being pulverized by beater grinder in proportion, through sterilization, cooling, insert that the solid microbe mixed culture ferments, after the fermentation ends by low temperature drying, get product after pulverizing.The present invention produces required active complex enzyme by the raw material that contains vegetable protein being carried out fermentation process, make to contain animal in the finished product, has improved the utilization rate of feed; And make in the product except that vegetable protein, increased the part mycoprotein, improved the protein content of raw material, improved the nutrition in the raw material.In addition owing to containing the effect that active complex enzyme composition that fermentation produces and growth factor have the feed digestive aid in this product.
The present invention compared with prior art, cost is low, technology is simple, cycle weak point, protein net added value height, feed nutrition are abundant, balanced, price of deed height, and can promote digestion.The non-medicine of this product, without any side effects.
The invention will be further described below in conjunction with embodiment.
The present invention mixes, adds the water mixing after at first various raw materials being pulverized by beater grinder in proportion, various material components and ratio range are: corn lees is 10-20%, corn germ cake is 10-20%, and dregs of beans is 20-30%, and corn protein powder is 30-40%.It is 1 that its water addition ratio is calculated by butt: 1-1.2.Through sterilization, cool off then.Its sterilising temp is 100 ℃, and sterilization time is 30 minutes.After will cultivating ripe solid microbe culture mixing more respectively, the inoculum concentration by 1% inserts in the mixed material that cools off the sterilization back, mixes thoroughly with mechanical means.Insert in the solid biologic reactor and cultivate, its condition of culture is as follows: temperature is 33 ℃ ± 2, and the time is 40 hours, and ventilation is that material per ton per hour ventilates 8000 cubic metres.After cultivating end, through pneumatic conveying drying, baking temperature is 75 ℃ with material, must not surpass 80 ℃, and the final moisture content of product is below 8%, and smashing fineness is the 60-80 order.
Said solid microbe mixture is formed through enlarging to cultivate by following bacterial classification:
Aspergillus niger Aspergillus niger
Aspergillus oryzae Aspergillus orgzae
Endomycopsis Fibnligera Endonycopsis fibuligera
Aspergillus awamori Aspergillus awamari
Aspergillus terricola Aspergillus terricola
Geotrichum candidum Geotrichum cadidum
Formosan Rhizopus Rhizopus formosensis
The present invention is all having greatly improved material component by fermentation aspect amino acid content and other nutrition component.Be component situation of change before and after the fermentation below:
Finished product after composition unit's fermenting raw materials
Protein % 43.5 51.3
Ash % 1.8 1.8
Moisture content % 3.5 4.5
Fat % 4.82 3.32
Crude fibre % 5.14 9.2
Total reducing sugar % 3.04 2.87
Reduced sugar % 2.32 2.87
V
AIu/100g 222 107
V
B1mg/100g 0.06 0.11
V
B2mg/100g 0.52 0.62
V
Cmg/100g 6.6 6.19
V
DMg/100g trace trace
V
Emg/100g 0.015 7.98
V
Kμg/100g 1.60 2.84
Plumbous PPm 4.68 6.5
Manganese PPm 9.90 21.1
Phosphorus PPm 1346.90 2515.0
Potassium PPm 2771.20 4121.2
Finished product after composition unit's fermenting raw materials
Sodium PPm 327.20 436.7
Calcium PPm 371.50 551.3
Magnesium PPm 798.30 1342.8
Copper PPm 11.70 4.9
Zinc PPm 20.00 21.0
Iron PPm 351.40 373.4
Selenium PPm 0.037 0.038
Asparatate g/100g 2.67 3.37
Threonine g/100g 1.47 1.81
Serine g/100g 2.22 2.58
Glutamic acid g/100g 9.68 9.34
Glycine g/100g 1.20 1.47
Alanine g/100g 4.02 4.13
Cystine g/100g 0.64 0.68
Valine g/100g 2.17 2.45
Methionine g/100g 0.82 0.91
Isoleucine g/100g 1.90 2.17
Leucine g/100g 7.50 7.09
Finished product after composition unit's fermenting raw materials
Tyrosine g/100g 1.86 1.95
Phenylalanine g/100g 2.73 2.89
Lysine g/100g 0.93 1.18
Ammonia g/100g 0.64 0.72
Histidine g/100g 0.83 0.91
Arginine g/100g 2.01 2.39
Proline g/100g 4.04 3.70
Tryptophan g/100g 0.14 0.29
Total amino acid content g/100g 47.1 50.6
AFB μ g/kg does not detect
Pathogenic bacteria-
Staphylococcus aureus does not detect
Salmonella does not detect
It is the processing of other agricultural basic processing industry waste residue of raw material that the present invention is equally applicable to the corn, the waste residue that produces in producing as corn alcohol, corn high fructose syrup.By utilization, improve the protein content and the price of deed, so the present invention has suitable application prospects to these waste residues.
Claims (6)
1, a kind of feed complex enzyme-protein production method, mix, add the water mixing after it is characterized in that various raw materials are pulverized by beater grinder in proportion, through sterilization, cooling, insert that the solid microbe mixed culture ferments, after the fermentation ends by low temperature drying, get product after pulverizing.
2, production method according to claim 1 is characterized in that said various raw material proportioning adjusts according to each material component, and its ratio range is as follows:
Corn lees is 10-20%;
Corn germ cake is 10-20%;
Dregs of beans is 20-30%;
Corn protein powder is 30-40%;
Water addition ratio is pressed butt and is calculated 1: 1-1.2.
3, production method according to claim 1 and 2, the sterilising temp that it is characterized in that raw material is 100 ℃, sterilization time is 30 minutes.
4, production method according to claim 1 is characterized in that the solid microbe mixture is formed through enlarging to cultivate by following bacterial classification, and each bacterial classification is as follows:
Aspergillus niger Aspergillus niger
Aspergillus oryzae Aspergillus orgzae
Endomycopsis Fibnligera Endonycopsis fibuligera
Aspergillus awamori Aspergillus awamari
Aspergillus terricola Aspergillus terricola
Geotrichum candidum Geotrichum cadidum
Formosan Rhizopus Rhizopus formosensis
5, production method according to claim 1 is characterized in that the main body Zymolysis Equipment in this production method adopts mechanical ventilation type solid biochemical reactor, and fermentation temperature is 30 ℃, fermentation period 40 hours.
6, production method according to claim 1 is characterized in that drying process adopts low temperature drying technology in this production method, and baking temperature is 75 ℃, keeps the complex enzyme activity simultaneously again at sterilizing-drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91105141A CN1021761C (en) | 1991-08-01 | 1991-08-01 | Production method of feed complex enzyme-protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN91105141A CN1021761C (en) | 1991-08-01 | 1991-08-01 | Production method of feed complex enzyme-protein |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1059260A true CN1059260A (en) | 1992-03-11 |
| CN1021761C CN1021761C (en) | 1993-08-11 |
Family
ID=4907004
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN91105141A Expired - Fee Related CN1021761C (en) | 1991-08-01 | 1991-08-01 | Production method of feed complex enzyme-protein |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1021761C (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036891C (en) * | 1993-06-03 | 1998-01-07 | 山东大学 | Method for making basic feed for rabbits |
| CN1292673C (en) * | 2004-07-14 | 2007-01-03 | 哈尔滨中冠生物工程有限公司 | Process for preparing active peptide of corn protein |
| CN102210388A (en) * | 2011-07-27 | 2011-10-12 | 河北联合大学 | Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction |
| CN102406066A (en) * | 2011-10-11 | 2012-04-11 | 中北大学 | Feed additive for dairy cow and preparation method thereof |
| CN102406060A (en) * | 2011-10-11 | 2012-04-11 | 中北大学 | Feed additive and production method thereof |
| CN101427782B (en) * | 2008-11-27 | 2012-05-30 | 赵敏 | Bifiobacterium beverage of corn and preparation method thereof |
| CN103652327A (en) * | 2013-12-10 | 2014-03-26 | 青岛琅琊台集团股份有限公司 | Biological feed produced by wastes |
| CN104621343A (en) * | 2014-12-25 | 2015-05-20 | 山东西王糖业有限公司 | Production method of liquid feed |
| CN106376724A (en) * | 2016-08-30 | 2017-02-08 | 惠州市瑰拉科技有限公司 | Corn meal fermentation processing method |
-
1991
- 1991-08-01 CN CN91105141A patent/CN1021761C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036891C (en) * | 1993-06-03 | 1998-01-07 | 山东大学 | Method for making basic feed for rabbits |
| CN1292673C (en) * | 2004-07-14 | 2007-01-03 | 哈尔滨中冠生物工程有限公司 | Process for preparing active peptide of corn protein |
| CN101427782B (en) * | 2008-11-27 | 2012-05-30 | 赵敏 | Bifiobacterium beverage of corn and preparation method thereof |
| CN102210388A (en) * | 2011-07-27 | 2011-10-12 | 河北联合大学 | Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction |
| CN102210388B (en) * | 2011-07-27 | 2012-07-04 | 河北联合大学 | Method for preparing feed additive by using buckwheat flower and leaf wastes after flavone extraction |
| CN102406066A (en) * | 2011-10-11 | 2012-04-11 | 中北大学 | Feed additive for dairy cow and preparation method thereof |
| CN102406060A (en) * | 2011-10-11 | 2012-04-11 | 中北大学 | Feed additive and production method thereof |
| CN102406066B (en) * | 2011-10-11 | 2013-01-16 | 中北大学 | Feed additive for dairy cow and preparation method thereof |
| CN103652327A (en) * | 2013-12-10 | 2014-03-26 | 青岛琅琊台集团股份有限公司 | Biological feed produced by wastes |
| CN103652327B (en) * | 2013-12-10 | 2015-04-01 | 青岛琅琊台集团股份有限公司 | Biological feed produced by wastes |
| CN104621343A (en) * | 2014-12-25 | 2015-05-20 | 山东西王糖业有限公司 | Production method of liquid feed |
| CN106376724A (en) * | 2016-08-30 | 2017-02-08 | 惠州市瑰拉科技有限公司 | Corn meal fermentation processing method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1021761C (en) | 1993-08-11 |
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