CN105925477B - Bioreactor and its multi-point pollutant real-time online continuous monitor system - Google Patents
Bioreactor and its multi-point pollutant real-time online continuous monitor system Download PDFInfo
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- CN105925477B CN105925477B CN201610276492.6A CN201610276492A CN105925477B CN 105925477 B CN105925477 B CN 105925477B CN 201610276492 A CN201610276492 A CN 201610276492A CN 105925477 B CN105925477 B CN 105925477B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The present invention discloses a kind of bioreactor and its multi-point pollutant real-time online continuous monitor system, including bioreactor ontology and the sealing cover in bioreactor ontology upper and lower ends is set, bioreactor chamber body is the biochemical reaction chamber for degradation of contaminant, biochemical reaction chamber is equipped with several honeycomb sandwich board groups from top to bottom, for by biochemical reaction chamber by being highly separated into multilayer, every layer is equipped with sample tap, each honeycomb sandwich board group is made of two pieces of porous clamping plates, collection gas coil pipe is equipped between two pieces of porous clamping plates, the sample tap of bioreactor ontology is stretched into the outlet end of collection gas coil pipe, collection gas coil pipe is used to collect the contaminant gases of each layer.Real-time online is carried out using the multi-point pollutant of the bioreactor continuously to monitor.The present invention can be sampled different points, and ensure that the acquisition accuracy of information at each sample point is high, by being continuously monitored to multi-point pollutant, obtain the changing rule during contaminant degradation.
Description
Technical field
The present invention relates to experimental facilities, belong to the contaminant degradation technical field of Biomedia, and in particular to biological respinse
Device and its multi-point pollutant real-time online continuous monitor system.
Background technology
Household refuse landfill sites stabilization procedures generate landfill gas include 55%~60% methane, the two of 40%~45%
Carbonoxide and a certain amount of volatility non-methane type organic（NMOCs）Such as chlorohydrocarbon.Studies at home and abroad show that methane, chloro
The volatile organic contaminants such as hydrocarbon produce great influence as important greenhouse gases, to environmental change.With sustainable development
Requirement of the proposition of exhibition to ecological environment is higher and higher.It is warm caused by how effectively eliminating methane, chlorohydrocarbon and pollutions being waited to slow down
Room effect has become the important research content of field of environment protection.
Covering layer of landfill site has tamed derived from process a large amount of microorganism for a long time in landfill gas, research shows that this is slightly raw
Object can effectively remove the environmental contaminants such as methane, volatile chlorinated hydrocarbon in landfill gas, therefore using overburden soil as Biomedia
There are very big potentiality in methane emission reduction and contaminant degradation field.It is carried out at present using the bioreactor of simulation cover sheet form
Methane biologic oxidation carries out chlorohydrocarbon biodegradation etc. using overburden soil bioreactor.In the research of simulation coating or experiment
In the small-sized contaminant degradation reactor of room, the monitoring process of different point pollutants relies primarily on artificial right place and extracts,
It is sent into detector detection, the process takes a significant amount of time and energy for operators.When pollutant flux is constant,
During diffusing up, the ladder of stable law is presented in continuous coating blinding microbial degradation, the pollutant concentration at different gradients
Degree variation.But for truth, pollutant diffusion flux constantly changes, and system is difficult to realize stable state, passes through
Syringe takes gas in the outlet of reactor, and since pollutant concentration constantly changes, and biogas is uneven in reactor cross direction profiles,
The taken pollutant concentration of syringe cannot represent the mean concentration at a certain gradient, and the sampling of contaminant degradation process is caused to lack
Homogeneity, some point for thus only monitoring some time cannot achieve the correct understanding of contaminant degradation, cannot continuously monitor dirt
Contaminate object degradation process in changing rule, discontinuous monitoring process it is difficult to ensure that data confidence level, it is difficult to realize biogas and
The accurate measurements of pollutant concentration, caused by erroneous judgement seriously affected research process.
Invention content
An object of the present invention is for overcome the deficiencies in the prior art, to provide a kind of bioreactor, and the biology is anti-
It answers device to be equipped with multiple monitoring sites, different points can be sampled, and ensure that the acquisition information at each sample point is accurate
Degree is high.
The second object of the present invention is to provide a kind of multi-point pollutant real-time online of bioreactor and continuously monitor to be
System, continuously monitors multi-point pollutant, obtains the changing rule during contaminant degradation.
An object of the present invention can be achieved through the following technical solutions：
A kind of bioreactor, including bioreactor ontology and the sealing in bioreactor ontology upper and lower ends is set
Lid, feature are：The bioreactor chamber body is the biochemical reaction chamber for degradation of contaminant, the biochemical reaction
Chamber is equipped with several honeycomb sandwich board groups from top to bottom, for by biochemical reaction chamber, by multilayer is highly separated into, every layer to be equipped with sampling
Mouthful, each honeycomb sandwich board group is made of two pieces of porous clamping plates, and collection gas coil pipe, the gas collection are equipped between two pieces of porous clamping plates
The sample tap of bioreactor ontology is stretched into the outlet end of coil pipe, and the collection gas coil pipe is used to collect the contaminant gases of each layer；
The sealing cover of bioreactor ontology lower end is equipped with the pollutant feed inlet communicated with biochemical reaction chamber, bioreactor
The sealing cover of ontology upper end is equipped with the exhaust outlet and air intlet communicated with biochemical reaction chamber.The bioreactor uses cylinder
Shape bioreactor.
The collection gas coil pipe is porous spiral coil, and the porous spiral coil is made of hollow spiral coil, described
Hollow spiral coil is equipped with several gas collection holes, and the aperture of the porous clamping plate of aperture ratio of the gas collection hole is small, the gas collection hole
Aperture be less than or equal to 0.2 mm.
The biochemical reaction chamber lower end of the bioreactor ontology is additionally provided with pollutant and diffuses chamber.The pollutant diffuses chamber
Granular sandstone in interior filling.
The biochemical reaction cavity outer wall of the bioreactor ontology is equipped with the temperature control interlayer for adjusting temperature.
The second object of the present invention can be achieved through the following technical solutions：
A kind of multi-point pollutant real-time online continuous monitor system of bioreactor, feature are：Including sampling
System, air-path control system, continuous monitor system and data processing system, the sampling system are appointed using Claims 1-4
One bioreactor, the sampling system is for being sampled pollutant at each gradient of bioreactor；The biology
Each sample tap of reactor is connected with each sampling pipe of air-path control system respectively, and each sampling pipe is connected in parallel on automatic transfer valve
Each connection position, the air-path control system is used to control by automatic transfer valve to be connected probe tube with each sample tap of sampling system
The break-make in road switches；The six-way valve injector phase that the outlet end of the automatic transfer valve passes through sample channel and continuous monitor system
Even, the six-way valve injector is connected with the injection port of detector, and the continuous monitor system is used for through detector to certain
Each sample tap pollutant sample of amount carries out ingredient and Concentration Testing；At the input/output terminal and data of the continuous monitor system
The calculating mechatronics of reason system, the data processing system obtain continuous monitor system for controlling continuous monitor system
The contaminant detection results taken carry out Data Analysis Services and show analysis result.
The air-path control system includes gas circuit control panel, controller, automatic transfer valve, relay, the gas circuit control
The sampling pipe of a plurality of parallel connection is set on panel processed, and every sampling pipe is both connected in each connection position of automatic transfer valve, often
The first on-off valve and inlet suction port are equipped on sampling pipe, each inlet suction port corresponds to first on-off valve；The control
Device processed is electrically connected with relay, and relay is electrically connected with automatic transfer valve, and the controller is automatic for controlling by relay
The break-make of each connection position of switching valve switches, and ensures that only there are one connection positions to open for automatic transfer valve when detection.
The continuous monitor system is equipped with control unit, and the control unit of the continuous monitor system passes through relay and six
Port valve injector is electrically connected, the sampling sample introduction for controlling six-way valve injector, and quantity tube is equipped in the six-way valve injector.
It is connected with asepwirator pump on the six-way valve injector, the control unit of the asepwirator pump and continuous monitor system is electrically connected
It connects, it is described for ensureing at the different gradients in bioreactor that pollutant can smoothly enter into sampling pipe and then enter quantity tube
The outlet end of asepwirator pump is connected with waste gas recovering device.
The pollutant feed inlet of the bioreactor by pollutant inlet suction port be connected with for pollutant container
Connected pollutant transportation pipeline, the pollutant transportation pipeline are equipped with second flowmeter and the second on-off valve；The biology
The air intlet of reactor is connected with the airflow pipe for being connected with compressed air container, institute by air inlet connector
It states airflow pipe and is equipped with third on-off valve；The exhaust outlet of the bioreactor is equipped with first flowmeter.
The sampling system is equipped with multiple bioreactors, and the sample tap of each bioreactor, which is connected in parallel on by branch pipe, to be taken
On sample pipeline, the degradation situation for monitoring each bioreactor pollutant at different conditions.
Beneficial effects of the present invention：Several honeycomb sandwich board groups are arranged in the biochemical reaction intracavitary of bioreactor of the present invention, will
Biochemical reaction chamber is by multilayer is highly separated into, and every layer is correspondingly arranged a sample tap, and each honeycomb sandwich board group is by more than two pieces
Hole clamping plate is constituted, and collection gas coil pipe is equipped between two pieces of porous clamping plates, the collection gas coil pipe is between two pieces of porous clamping plates of collection
Pollutant, sample tap is stretched into each outlet end for collecting gas coil pipe；Between the porous clamping plate in two pieces of each layer upper end of gas collection coil pickup
Contaminant gases so as to collect the contaminant gases of bioreactor difference gradient, and solve pollutant biological anti-
Answer the problem of cross direction profiles unevenness in device.The configuration of the present invention is simple improves the validity of detection sample.
The collection gas coil pipe is porous spiral coil, and the porous spiral coil is made of hollow spiral coil, described
Hollow spiral coil is equipped with several gas collection holes.Several gas collection holes being arranged on hollow spiral coil are by two pieces of porous clamping plates
Between contaminant gases be collected after remix to obtain uniformly mixed contaminant gases, realize different gradient biogas and
The uniform mixing of pollutant concentration.
The biochemical reaction chamber lower end of the bioreactor is additionally provided with pollutant and diffuses chamber, is passed through the pollution of bioreactor
Object enters biochemical reaction chamber after mixing by being carried out in diffusing chamber in pollutant, improves reaction effect.Pollutant diffuses
Granular sandstone improves mixed effect in intracavitary filling.
The outer wall of the bioreactor is equipped with the temperature control interlayer for adjusting temperature.Temperature control control by layer difference gradient is anti-
Device temperature is answered, the activity of overburden soil microorganism is enhanced, realizes overburden soil microbe efficient degradation.
The multi-point pollutant real-time online continuous monitor system of bioreactor of the present invention is used in bioreactor
The landfill yard overburden soil of biochemical reaction intracavitary filling energy degradation of contaminant, pollution is passed through to the biochemical reaction chamber of bioreactor
Bio-degradation reactions occur during diffusing up for object, pollutant, and bioreactor difference gradient is acquired by sampling system
Pollutant sample；The sampling pipe that is connected with bioreactor different height pollutant sample is controlled by air-path control system
Break-make switches, and ensures that the wherein connection position of a sampling pipe and automatic transfer valve for there was only bioreactor when detecting connects
Logical, the connection position of the sampling pipe of pollutant and automatic transfer valve disconnects at other gradients of the bioreactor；Sampling system
Bioreactor through air-path control system and continuous monitor system, by continuous monitor system to each gradient of bioreactor
A certain amount of pollutant sample carries out ingredient and Concentration Testing；The pollutant that continuous monitor system is obtained by data processing system
Testing result carries out Data Analysis Services and shows analysis result.It can realize continuously, easily and efficiently in different time sections pair
The pollutant concentration of bioreactor difference gradient is continuously detected, and the accuracy of monitoring is improved, and reflects practical landfill
The concentration variation of volatile contaminant, determines pollution palliating degradation degree and bioreactor operating status with this, can obtain in
Degradation rule of the pollutant concentration in continuous change procedure has weight for the contaminant degradation situation in evaluation refuse landfill
It acts on.
Description of the drawings
Fig. 1 is the structural schematic diagram of bioreactor of the present invention；
Fig. 2 is enlarged drawing at A in Fig. 1；
Fig. 3 is the structural schematic diagram of the porous clamping plate of the present invention
Fig. 4 is the structural schematic diagram of present invention collection gas coil pipe；
Fig. 5 is the functional block diagram of the multi-point pollutant real-time online continuous monitor system of bioreactor of the present invention；
Fig. 6 is the structural schematic diagram of the multi-point pollutant real-time online continuous monitor system of bioreactor of the present invention；
Fig. 7 is the air-path control system structural schematic diagram of a bioreactor in specific embodiment 1；
Fig. 8 is the biodegradation of methane；
Fig. 9 is chlorohydrocarbon and methane changes with time biodegradable figure；
Figure 10 is the air-path control system structural schematic diagram of multiple bioreactors in specific embodiment 2.
Specific implementation mode
The present invention will be further described below with reference to the drawings.
Specific embodiment 1：
Referring to a kind of Fig. 1 to bioreactor shown in Fig. 9, including bioreactor ontology 1 and setting are in biological respinse
The sealing cover of 1 upper/lower terminal of device ontology, sealing cover and the bioreactor ontology 1 of upper/lower terminal are all made of flanged joint side
Formula.The bioreactor ontology 1 can be that tubular constitutes tubular reactor (cylindrical reactor), can also be other geometric forms
Shape biology.1 inner cavity of bioreactor ontology is biochemical reaction chamber 2 for degradation of contaminant, in the biochemical reaction chamber 2
The microorganism overburden soil filler of degradation of contaminant or the granular filler of other degradation of contaminant, the microorganism are set
Overburden soil filler comes from refuse landfill overburden soil.The biochemical reaction chamber 2 is equipped with several honeycomb sandwich board groups 3 from top to bottom,
For biochemical reaction chamber to be equipped with multiple sample tap 1-1 by multilayer, bioreactor ontology 1 is highly separated into, every layer right
One sample tap 1-1 should be set, and each sample tap is equipped with outwardly extending connector, and each honeycomb sandwich board group 3 is by two pieces
Porous clamping plate is constituted, and two pieces of porous clamping plates are identical as the biochemical reaction chamber cross section size shape of bioreactor, make more
Hole clamping plate is supported on the biochemical reaction chamber of bioreactor just, can also be set in the biochemical reaction intracavitary of the bioreactor
It is useful for supporting the supporting block of porous clamping plate, porous clamping plate is made to be supported in the supporting block of biochemical reaction chamber.The porous clamping plate
The granularity of aperture ratio overburden soil filler or granular filler is small, and porous clamping plate may be used PVC material and be made.More than two pieces
Collection gas coil pipe 4 is equipped between the clamping plate of hole, the sample tap 1-1 of bioreactor ontology, institute are stretched into the outlet end of the collection gas coil pipe 4
Collection gas coil pipe 4 is stated for the contaminant gases between two pieces of porous clamping plate of each layer；The collection gas coil pipe 4 is porous spiral coil,
The porous spiral coil is made of hollow spiral coil, and the hollow spiral coil is equipped with several gas collection hole 4-1, institute
The aperture for stating the porous clamping plate of aperture ratio of gas collection hole 4-1 is small, and the aperture of the gas collection hole 4-1 is less than or equal to 0.2 mm.It is described
The sealing cover of 1 lower end of bioreactor ontology is equipped with the pollutant feed inlet 5 communicated with biochemical reaction chamber 2, bioreactor
The sealing cover of 1 upper end of ontology is equipped with exhaust outlet 6, the air intlet 7 communicated with biochemical reaction chamber 2, may be provided for preventing
The only spare stomata of air-hole blockage.The exhaust outlet 6 is used to be discharged and detect air-flow by the gas that bio-degradation reactions generate
Amount accounts degradation efficiency.The air intlet 7 is used to provide necessary oxygen for bio-degradation reactions to simulate practical landfill yard
State.In the present embodiment：The bioreactor ontology 1 uses cylindrical body.
Further：2 lower end of biochemical reaction chamber of the bioreactor ontology 1 is additionally provided with pollutant and diffuses chamber 9.It is described
Pollutant feed inlet 5 diffuses chamber 9 with the pollutant of bioreactor ontology 1 and communicates.Pollutant diffuses graininess in the interior filling of chamber 9
Sandstone, to improve mixed effect.
Further, 2 outer wall of biochemical reaction chamber of the bioreactor ontology 1 is equipped with the temperature control for adjusting temperature and presss from both sides
Water inlet is arranged in layer 8, described 8 one end of temperature control interlayer, and the other end is arranged discharge outlet, passes through temperature control control by layer bioreactor
Reaction temperature.
A kind of multi-point pollutant real-time online continuous monitor system using bioreactor of the present invention, including sample train
System 11, air-path control system 12, continuous monitor system 13 and data processing system 14, the sampling system 11 are wanted using right
1 to 4 any bioreactor is sought, the sampling system 11 is for taking pollutant at each gradient of bioreactor
Sample, the gradient refer to the different height of bioreactor, i.e. each layer contaminant gases of bioreactor.The bioreactor
Biochemical reaction chamber 2 in be filled with the landfill yard overburden soil of degradation of contaminant, contain degradation of contaminant in the landfill yard overburden soil
Microorganism, the pollutant feed inlet 5 of the bioreactor is connected with by pollutant inlet suction port for holding with pollutant
The connected pollutant transportation pipeline 23 of device, the pollutant inlet suction port use quick coupling.The pollutant transportation pipeline 23
It is equipped with second flowmeter 24 and the second on-off valve 25；Second flowmeter 24 is connected with the controller of air-path control system, passes through
The controller controls second flowmeter 24.Automatic on-off valve may be used in second on-off valve 25, and automatic on-off valve is controlled with gas circuit
The controller of system 12 is connected, or uses manual on-off valve.The pollutant transportation pipeline 23, second flowmeter 24 and second
On-off valve 25 is mounted on the gas circuit control panel 10 of air-path control system 12.In the present embodiment：The pollutant is chlorohydrocarbon
With the mixture of methane, the pollutant container uses chlorohydrocarbon steel cylinder and methane steel cylinder, on chlorohydrocarbon steel cylinder and methane steel cylinder
It is respectively equipped with pressure reducing valve, the pressure reducing valve is connected with pollutant transportation pipeline 23, the connected pollutant transportation pipe of chlorohydrocarbon steel cylinder
5 phase of pollutant feed inlet with bioreactor after the connected pollutant transportation pipeline 23 of road and methane steel cylinder is mixed by threeway
Even, the mixture of chlorohydrocarbon and methane is passed through into bioreactor.The pollutant that chlorohydrocarbon steel cylinder is connected with methane steel cylinder is defeated
It send and second flowmeter 24 and the second on-off valve 25 is respectively set on pipeline 23, the chlorohydrocarbon steel cylinder and methane steel cylinder pass through respectively
Second flowmeter 24 adjusts respective flow velocity, so as to adjust the mixing ratio of chlorohydrocarbon and methane, obtains the pollutant of different proportion.
The air intlet 7 of the bioreactor is connected with by air inlet connector for being connected with compressed air container
Airflow pipe 26, the air inlet connector use quick coupling.The airflow pipe 26 is logical equipped with third
Automatic on-off valve, the controller phase of automatic on-off valve and air-path control system may be used in disconnected valve 27, the third on-off valve 27
Even, or using manual on-off valve.Airflow pipe 26 and third on-off valve 27 are mounted on the gas circuit of air-path control system 12
On control panel 10.In the present embodiment：Compressed air container uses tank of compressed air, the air intlet of bioreactor to pass through
Airflow pipe 26 is connected with tank of compressed air.
The exhaust outlet 6 of the bioreactor is equipped with first flowmeter.The first flowmeter is for calculating pollutant
Oxidation efficiency, oxidation efficiency=（Pollutant feed inlet flow * pollutants feed inlet concentration-exhaust outlet flow * exhaust outlet concentration）/
（Pollutant feed inlet flow * pollutant feed inlet concentration）.
Each sample tap 1-1 of the bioreactor is connected with each sampling pipe 15 of air-path control system 12 respectively, respectively
Sampling pipe 15 is connected in parallel on each connection position of automatic transfer valve 16, the air-path control system 12 include gas circuit control panel 10,
Controller, automatic transfer valve 16, relay, are arranged the sampling pipe 15 of a plurality of parallel connection on the gas circuit control panel 10, every
Sampling pipe 15 is both connected in each connection position of automatic transfer valve 16, and the first on-off valve 21 is equipped on every sampling pipe 15
With inlet suction port 22, each inlet suction port 22 corresponds to first on-off valve 21, and first on-off valve 21 may be used automatically
On-off valve, automatic on-off valve are connected with controller, or use manual on-off valve, and the inlet suction port 22 uses quick coupling.
Sampling pipe 15 is connect by connecting pipe with the joint sealing of sample tap 1-1 by inlet suction port 22, the sampling pipe, company
Hose or other pipelines may be used in take over road.The controller is electrically connected with relay, relay and 16 electricity of automatic transfer valve
It connects, the break-make that the controller is used to control 16 each connection position of automatic transfer valve by relay switches, when ensureing to detect certainly
There are one connection positions to open for dynamic switching valve 16, to control the periodical open and-shut mode of each sampling pipe.The controller can
To use PLC controller or other existing controllers.In the present embodiment：The relay uses the relay of JGX-20F models.
The automatic transfer valve uses 00148701 model automatic transfer valve of BURKERT producers.The air-path control system 12 is used for
The break-make switching for the sampling pipe 15 that is connected with 11 each sample tap 1-1 of sampling system is controlled by automatic transfer valve 16.
The six-way valve injector that the outlet end of the automatic transfer valve 16 passes through sample channel 17 and continuous monitor system 13
18 are connected, and the six-way valve injector 18 is connected with the injection port of detector 19, the continuous monitor system include six-way valve into
Sample device 18, asepwirator pump 20, control unit, detector, the outlet end of the automatic transfer valve 16 by sample channel 17 with it is continuous
The six-way valve injector 18 of monitoring system is connected, and the six-way valve injector 18 is connected with the injection port of detector 19, and described six
Quantity tube is equipped in port valve injector 18, the capacity of quantity tube can be 0.5 ml either 1 ml or 2 ml.Described six is logical
Valve injection device 18 is by being electrically connected with control unit, the sampling sample introduction for controlling six-way valve injector 18.Six-way valve injector
Asepwirator pump 20 is connected on 18, the asepwirator pump 20 is electrically connected with control unit, for ensureing at the different gradients in reactor
Pollutant can smoothly enter into sampling pipe 15 and then enter quantity tube, and the asepwirator pump 20 ensure that air pressure is very in the bioreactor
Hour, pollutant is entered the detector 19 of continuous monitor system by collection gas coil pipe through air-path control system.The asepwirator pump 20
Outlet end is connected with waste gas recovering device, and the waste gas recovering device avoids losing for being recycled contaminant gases
Laboratory is drained to, laboratory environment is influenced.The sample holes of the six-way valve injector 18 are connected with the outlet end of automatic transfer valve,
In the present embodiment：The six-way valve injector 18 using 12H-0600V models six-way valve, 3,6 ports of six-way valve with it is quantitative
Pipe is connected, and 4 ports of six-way valve are connected with asepwirator pump, and 1,2 ports of six-way valve are connected with the injection port of detector, six-way valve
5 ports are connected with the outlet end of automatic transfer valve.The detector 19 is exactly gas chromatographic detection instrument, the gas chromatographic detection
Instrument includes thermal conductivity detector (TCD) and electron capture detector.PLC controller or other existing controls may be used in described control unit
Device.The continuous monitor system 13 is used to carry out ingredient and concentration to a certain amount of each sample tap pollutant sample by detector
Detection.
The input/output terminal of the continuous monitor system 13 and the calculating mechatronics of data processing system 14, the data
Processing system 14 is for controlling continuous monitor system 13, and the contaminant detection results obtained to continuous monitor system 13 are into line number
According to analyzing processing and show analysis result.There are data processing system and control software in the computer of data processing system 14, even
The detector 19 of continuous monitoring system 13 is output to the data processing system of computer with mechatronics, the result of detector 19 is calculated
It unites, the control software in simultaneous computer controls detector.
Control of the computer of data processing system 14 also with the control unit of continuous monitor system and air-path control system 12
Device electrical connection processed, the control software of the computer of data processing system control the control unit and gas circuit of continuous monitor system respectively
The controller of control system, the computer of data processing system are used to control the probe tube of each air-path control system of different time
The analysis detection time of road folding condition, sample time and continuous monitor system and the cycle of operation etc., and detector is obtained
Contaminant detection results carry out Data Analysis Services and show analysis result.
In the present embodiment：The biochemical reaction chamber of the bioreactor is separated into six layers, and every layer is correspondingly arranged a feeding
Mouth 1-1, six material taking mouth 1-1 of respectively A1, A2, A3, A4, A5, A6 material taking mouth 1-1, bioreactor pass through gas circuit respectively
Six sampling pipes 15 of control system 12 are connected with six connection positions of automatic transfer valve 16, and A1 material taking mouths, which correspond to, to automatically switch
The No.1 of valve 16 connects, and A2 material taking mouths correspond to No. two connections of automatic transfer valve 16, and A3 material taking mouths correspond to automatic transfer valve 16
No. three connections, A4 material taking mouths correspond to No. four connections of automatic transfer valve 16, and A5 material taking mouths correspond to No. five companies of automatic transfer valve 16
It connects, A6 material taking mouths correspond to No. six connections of automatic transfer valve 16.It is controlled and is automatically switched by the controller of air-path control system 12
The break-make of 16 each connection position of valve, the outlet end of automatic transfer valve 16 are connected with the six-way valve injector 18 of continuous monitor system 13,
The contaminant gases of acquisition detector 19 is sent by six-way valve injector 18 to be detected.
Using the method for the multi-point pollutant real-time online continuous monitor system of bioreactor of the present invention：
First；The microorganism overburden soil of degradation of contaminant, the degradation are filled in the biochemical reaction intracavitary of bioreactor
The microorganism overburden soil of pollutant is derived from the overburden soil of household refuse landfill sites, is rich in multiple functions microorganism, methane of such as degrading
Isothermal chamber gas, the pollutants such as chlorohydrocarbon.Led to from the bottom up to biochemical reaction chamber by the pollutant feed inlet of bioreactor
Enter pollutant, air is passed through to biochemical reaction chamber by the air intlet of bioreactor from top to bottom, the flow velocity of air is general
Control is in 2 ml/min.Bio-degradation reactions occur during diffusing up for pollutant, anti-by sampling system acquisition biology
Answer the pollutant sample of device difference gradient；The pollution into the pollutant feed inlet of bioreactor is adjusted by second flowmeter
Logistics speed, gas flux are generally 0.2-125 molm-2·d-1。
Then：The break-make switching that the sampling pipe that is connected with bioreactor is controlled by air-path control system, ensures examining
A wherein sampling pipe for bioreactor difference gradient contaminant gases is connected to the connection position of automatic transfer valve when survey, should
The connection position of the sampling pipe of pollutant and automatic transfer valve disconnects at other gradients of bioreactor.
Again：The a certain amount of pollutant sample of sample tap at each gradient of bioreactor is carried out by continuous monitor system
Ingredient and Concentration Testing；When the pollutant concentration at continuous monitor system detects each gradient of bioreactor is constant（In life
When object reactor bottom is passed through a fixed pollutant concentration, if pollutant concentration value at each gradient is not at any time
Change and change, then illustrates reaction up to balance, i.e., concentration is constant.Generally 2-3 hours）, up to balance, change pollutant is logical for reaction
Amount, then controls the break-make of each sampling pipe, by continuous monitor system to each sampling pipe again by air-path control system
Interior pollutant is detected.Assuming that automatic transfer valve switching time is 15 min, the asepwirator pump interval time of continuous monitor system
For 14 min, 50 s, asepwirator pump is worked prior to automatic transfer valve to ensure that asepwirator pump polluted the last time left in sample channel
Object evacuating air, asepwirator pump generally shift to an earlier date 10 s operations.
Finally：The contaminant detection results that continuous monitor system obtains are carried out at data analysis by data processing system
It manages and shows analysis result.As can be seen that methane concentration subtracts rapidly with the increase of gradient from continuous monitoring figure Fig. 8 of methane
Small, oxygen increases with gradient and is increased, and the methane oxidation ability at 0-30 cm is maximum, with the change of initial methane concentration,
Stable state changes.As can be seen from Figure 9 the degradation results in the reactor of chlorohydrocarbon and methane, change over time
Chlorohydrocarbon and methane are degraded rich in microorganism overburden soil.
The present invention realizes the uniform accurate collection of pollutant in bioreactor, can be to any point in bioreactor
The pollutant concentration of position continuously monitors, and improves the flexibility in the application of pollutant monitoring technology；Because in landfill yard
Pollutant changes very greatly in the concentration of different phase, and the palliating degradation degree of pollutant is difficult to control, for pollutant flux in reality
Constantly change in place, the accuracy of monitoring can be improved using multi-point pollutant real-time online continuous monitor system, can be filled
Divide the changing rule for seeing clearly pollutant.It, can be targetedly in the difference of landfill yard landfill after the degradation rule for obtaining pollutant
Stage, by increasing biological reinforced (sprinkling bacterium solution or culture medium etc.) or without enhancement microbiological activity, realizing to landfill yard dirt
Contaminate effective control of object.
Specific embodiment 2：
Shown in Figure 10, the characteristics of the present embodiment, is：The sampling system 11 is equipped with multiple bioreactors, each biology
The sample tap 1-1 of reactor is connected in parallel on by branch pipe on sampling pipe 15, for monitoring each bioreactor in different condition
The degradation situation of lower pollutant, such as：Each bioreactor is monitored in different temperatures, different types of overburden soil, different chlorohydrocarbons
With the contaminant degradation situation under the conditions of the concentration mixing ratio of methane, which can control multiple biologies simultaneously
Reactor（A、B、C、D……）Supply, and do not influence each other between each reactor.In the present embodiment：Sampling system 11 is set
There are tetra- bioreactors of A, B, C, D, the degradation condition of tetra- bioreactors of A, B, C, D different.A, tetra- lifes of B, C, D
Each sample tap of object reactor is connected in parallel on by branch pipe on the corresponding sampling pipe of each gradient 15 respectively, and each branch pipe is equipped with and takes
Sample mouth connected inlet suction port and the first on-off valve, by detecting each sample tap pollutant concentration of four bioreactors,
Obtain the degradation rule of landfill yard overburden soil.Other features are identical as specific embodiment 1.
Claims (4)
1. a kind of multi-point pollutant real-time online continuous monitor system of bioreactor, feature are：Including sample train
System（11）, air-path control system（12）, continuous monitor system（13）And data processing system（14）, the sampling system（11）
The bioreactor of use, including bioreactor ontology（1）With setting in bioreactor ontology（1）The sealing of upper and lower ends
Lid, the bioreactor ontology（1）Inner cavity is the biochemical reaction chamber for degradation of contaminant（2）, the biochemical reaction chamber（2）
It is equipped with several honeycomb sandwich board groups from top to bottom（3）, for by biochemical reaction chamber, by multilayer is highly separated into, every layer to be equipped with sampling
Mouthful（1-1）, each honeycomb sandwich board group（3）It is made of two pieces of porous clamping plates, collection gas coil pipe is equipped between two pieces of porous clamping plates
（4）, the collection gas coil pipe（4）Outlet end stretch into the sample tap of bioreactor ontology（1-1）, the collection gas coil pipe（4）With
In the contaminant gases for collecting each layer；The bioreactor ontology（1）The sealing cover of lower end is equipped with and biochemical reaction chamber（2）
The pollutant feed inlet communicated（5）, bioreactor ontology（1）The sealing cover of upper end is equipped with and biochemical reaction chamber（2）It communicates
Exhaust outlet（6）And air intlet（7）, the bioreactor ontology（1）Biochemical reaction chamber（2）Outer wall is equipped with for adjusting
The temperature control interlayer of temperature（8）, the temperature control interlayer（8）Water inlet is arranged in one end, and the other end is arranged discharge outlet, passes through temperature control interlayer
Control the reaction temperature of bioreactor, the bioreactor ontology（1）Biochemical reaction chamber（2）Lower end is additionally provided with pollutant
Diffuse chamber（9）, the pollutant feed inlet of the bioreactor（5）It is connected with and is used for and pollutant by pollutant inlet suction port
The connected pollutant transportation pipeline of container（23）, the pollutant container is using chlorohydrocarbon steel cylinder and methane steel cylinder, chlorohydrocarbon steel
Pressure reducing valve, the pressure reducing valve and pollutant transportation pipeline are respectively equipped on bottle and methane steel cylinder（23）It is connected, chlorohydrocarbon steel cylinder phase
The pollutant transportation pipeline that pollutant transportation pipeline even is connected with methane steel cylinder（23）After being mixed by threeway with biological respinse
The pollutant feed inlet of device（5）It is connected, the mixture of chlorohydrocarbon and methane, chlorohydrocarbon steel cylinder and first is passed through into bioreactor
The connected pollutant transportation pipeline of alkane steel cylinder（23）On second flowmeter is respectively set（24）With the second on-off valve（25）；The life
The air intlet of object reactor（7）It is connected with the air delivery pipe for being connected with compressed air container by air inlet connector
Road（26）, the airflow pipe（26）It is equipped with third on-off valve（27）；The exhaust outlet of the bioreactor（6）On set
There are first flowmeter, the sampling system（11）For being sampled to pollutant at each gradient of bioreactor；The biology
Each sample tap of reactor respectively with air-path control system（12）Each sampling pipe（15）It is connected, each sampling pipe（15）It is in parallel
In automatic transfer valve（16）Each connection position, the air-path control system（12）For passing through automatic transfer valve（16）It controls and takes
Sample system（11）Each sample tap（1-1）Be connected sampling pipe（15）Break-make switching；The automatic transfer valve（16）Outlet end
Pass through sample channel（17）With continuous monitor system（13）Six-way valve injector（18）It is connected, the six-way valve injector（18）
With detector（19）Injection port be connected, the six-way valve injector（18）It is interior to be equipped with quantity tube, the continuous monitor system
（13）Equipped with control unit, the continuous monitor system（13）Control unit pass through relay and six-way valve injector（18）Electricity
Connection, for controlling six-way valve injector（18）Sampling sample introduction, the six-way valve injector（18）On be connected with asepwirator pump
（20）, the asepwirator pump（20）With continuous monitor system（13）Control unit electrical connection, for ensureing in bioreactor
Pollutant can smoothly enter into sampling pipe at different gradients（15）And then enter quantity tube, the asepwirator pump（20）Outlet end connection
There are waste gas recovering device, the continuous monitor system（13）For by detector to a certain amount of each sample tap pollutant sample
Carry out ingredient and Concentration Testing；The continuous monitor system（13）Input/output terminal and data processing system（14）Computer
Electrical connection, the data processing system（14）For controlling continuous monitor system（13）, and to continuous monitor system（13）It obtains
Contaminant detection results carry out Data Analysis Services and showing analysis result.
2. the multi-point pollutant real-time online continuous monitor system of bioreactor according to claim 1, feature
It is：The collection gas coil pipe（4）For porous spiral coil, the porous spiral coil is made of hollow spiral coil, described
Hollow spiral coil is equipped with several gas collection holes（4-1）, the gas collection hole（4-1）The porous clamping plate of aperture ratio aperture it is small.
3. the multi-point pollutant real-time online continuous monitor system of bioreactor according to claim 1, feature
It is：The air-path control system（12）Including gas circuit control panel（10）, controller, automatic transfer valve（16）, relay, institute
State gas circuit control panel（10）The sampling pipe of the upper a plurality of parallel connection of setting（15）, every sampling pipe（15）It is both connected to and cuts automatically
Change valve（16）Each connection position on, every sampling pipe（15）On be equipped with the first on-off valve（21）And inlet suction port（22）, often
A inlet suction port（22）Corresponding first on-off valve（21）；The controller is electrically connected with relay, relay with cut automatically
Change valve（16）Electrical connection, the controller is by relay for controlling automatic transfer valve（16）The break-make of each connection position switches,
Ensure automatic transfer valve when detection（16）Only there are one connection positions to open.
4. the multi-point pollutant real-time online continuous monitor system of bioreactor according to claim 1, feature
It is：The sampling system（11）Equipped with multiple bioreactors, the sample tap of each bioreactor（1-1）Simultaneously by branch pipe
It is associated in sampling pipe（15）On, the degradation situation for monitoring each bioreactor pollutant at different conditions.
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