CN105920069A - Yeast microcapsule of globe artichoke polyphenol and preparation method of yeast microcapsule - Google Patents
Yeast microcapsule of globe artichoke polyphenol and preparation method of yeast microcapsule Download PDFInfo
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Abstract
The invention discloses a yeast microcapsule of globe artichoke polyphenol and a preparation method of the yeast microcapsule. A yeast microcapsule product is prepared from freeze-dried powder of globe artichoke serving as a core material and yeast cells serving as an embedding wall material through water bath constant-temperature oscillation, centrifuging and freeze-drying, wherein the freeze-dried powder of globe artichoke is obtained through extraction, purification and freeze-drying. Yeast cells are rich in nutrition self, and due to microencapsulation of the yeast cells, the antioxidant activity of polyphenol is maintained, and furthermore nutrient substances of yeast are supplied, so that the yeast microcapsule has the characteristics of slow medicine release, high bioavailability, relatively simple preparation process and is suitable for industrial large-scale production, and meanwhile ideas and directions are provided for further application of polyphenol.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to yeast microcapsule of a kind of Carlina acaulis polyphenol and preparation method thereof.
Background technology
Containing abundant polyphenol compound in Carlina acaulis stem and leaf, including caffeic acid (caffeic acid), chlorogenic acid (chlorogenic
Acid), rutin (rutinose), luteolin (cynaroside) etc., polyphenol compound is active component main in Carlina acaulis,
Medicine can be can act also as preventing and treating bile secretion deficiency and hepatitis as medicine, the raw material of health product.In recent years, study more
Concentrate on the preventing and treating of the chronic diseases such as Carlina acaulis active component antioxidation, blood fat reducing, prophylaxis of hypertension.Polyphenolic extract contains
A lot of polysaccharose substances, but its biological activity is the most unstable, limits its application.Use microcapsule technology can solve the problems referred to above.
Microcapsule is to utilize wall material, in taking certain method to be embedded in by core, is a kind of emblem packing technique, and this technology can change
The kind physical propertys such as the outward appearance of material, dissolubility that are embedded, improve the stability of core, it is possible to control the release of material.At present
Conventional microcapsule method mostly is the physico-chemical process such as complex coacervation and spray drying method, but all has chemical agent residue, equipment high
The problem such as expensive.
Summary of the invention
In place of it is an object of the invention to overcome the deficiencies in the prior art, it is provided that the yeast microcapsule of a kind of Carlina acaulis polyphenol and system thereof
Preparation Method, with the lyophilized powder of the extracted preparation of Carlina acaulis as core, yeast cells is embedding wall material, and the yeast preparing polyphenol is micro-
Capsule product, has the advantages that medicine slowly discharges, bioavailability is high, preparation technology is relatively simple, is suitable for industrialized great production,
The most also the application further for polyphenol provides thinking and direction.
One of the technical solution adopted for the present invention to solve the technical problems is:
The preparation method of the yeast microcapsule of a kind of Carlina acaulis polyphenol, in described yeast microcapsule, yeast cells is embedding wall material, court
Fresh Ji polyphenol is core, and this preparation method includes:
1) take the Carlina acaulis stem and leaf powder that particle diameter is 10～60 mesh, add the 35～98% of 8～45 times amount (quality-volume ratio)
(mass concentration) ethanol, 150～850W supersound extraction 1～4 times, each 20～200min；Filter, merging filtrate, dense
Contracting obtains the clear paste of relative density 1.23～1.48 at 50 DEG C, lyophilization, prepares Carlina acaulis polyphenol lyophilized powder；
2) take dry yeast, add 4.5～5.5% (mass concentration) NaCl solution of 18～22 times amount, 52～56 DEG C of water-bath vibrations
After 4.5～5.5h, 4000～6000rpm are centrifuged 8～12min, wash 1～3 time, lyophilization, complete yeast cells and locate in advance
Reason；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
(i.e. the mass ratio of Carlina acaulis polyphenol lyophilized powder and pretreated yeast cells) is that the ratio of 1:0.2～5 mixes, and adds 18～22
The water of times amount, at 25～75 DEG C, after water bath with thermostatic control vibration embedding 1～11h, 4000～6000rpm are centrifuged 8～12min, washing
2～4 times, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its embedding rate is 30～65%.
In one embodiment: described step 1) in, add 94～96% ethanol of 39～41 times amount, 780～820W supersound extraction
2～4 times, each 170～190min；Described step 3) in, core wall vibrates than for 1:3.8～4.2,68～72 DEG C of waters bath with thermostatic control
Embedding 9.5～10.5h；The microcapsule embedded rate of yeast obtained is 64～65%.
In one embodiment: described step 1) in, add 94～96% ethanol of 39～41 times amount, 380～420W supersound extraction
2～4 times, each 110～130min；Described step 3) in, core wall vibrates than for 1:3.8～4.2,58～62 DEG C of waters bath with thermostatic control
Embedding 9.5～10.5h；The microcapsule embedded rate of yeast obtained is 62～63%.
In one embodiment: described step 1) in, add 59～61% ethanol of 39～41 times amount, 780～820W supersound extraction
2～4 times, each 170～190min；Described step 3) in, core wall vibrates than for 1:3.8～4.2,48～52 DEG C of waters bath with thermostatic control
Embedding 3.5～4.5h；The microcapsule embedded rate of yeast obtained is 58～59%.
In one embodiment: described step 1) in, add 74～76% ethanol of 29～31 times amount, 380～420W supersound extraction
2～4 times, each 25～35min；Described step 3) in, core wall wraps than for 1:2.8～3.2,38～42 DEG C of water bath with thermostatic control vibrations
Bury 9.5～10.5h；The microcapsule embedded rate of yeast obtained is 48～49%.
In one embodiment: described step 1) in, add 79～81% ethanol of 39～41 times amount, 580～620W supersound extraction
1～3 time, each 110～130min；Described step 3) in, core wall vibrates than for 1:3.8～4.2,38～42 DEG C of waters bath with thermostatic control
Embedding 4.5～5.5h；The microcapsule embedded rate of yeast obtained is 59～60%.
In one embodiment: described step 1) in, add 59～61% ethanol of 39～41 times amount, 680～720W supersound extraction
2～4 times, each 25～35min；Described step 3) in, core wall wraps than for 1:1.8～2.2,48～52 DEG C of water bath with thermostatic control vibrations
Bury 7.5～8.5h；The microcapsule embedded rate of yeast obtained is 45～46%.
In one embodiment: described step 1) in, add 49～51% ethanol of 39～41 times amount, 380～420W supersound extraction
25～35min；Described step 3) in, core wall embeds 3.5～4.5h than for 1:3.8～4.2,58～62 DEG C of water bath with thermostatic control vibrations；
The microcapsule embedded rate of yeast obtained is 60～61%.
In one embodiment: described step 1) in, add 74～76% ethanol of 39～41 times amount, 480～520W supersound extraction
1～secondary, each 170～190min；Described step 3) in, core wall wraps than for 1:2.8～3.2,38～42 DEG C of water bath with thermostatic control vibrations
Bury 6.5～7.5h；The microcapsule embedded rate of yeast obtained is 50～51%.
The two of the technical solution adopted for the present invention to solve the technical problems are:
A kind of yeast microcapsule according to the Carlina acaulis polyphenol prepared by above-mentioned preparation method, in described yeast microcapsule, yeast cells
For embedding wall material, Carlina acaulis polyphenol is core, and its embedding rate is 30～65%.
The technical program is compared with background technology, and it has the advantage that
The present invention utilizes different osmotic pressuries, makes Carlina acaulis polyphenol penetrate yeast cell wall and enters intracellular, is formed with yeast cells
It is the microcapsule of core for embedding wall material, Carlina acaulis polyphenol, had both maintained the antioxidant activity of polyphenol, it may have yeast itself rich
Rich nutritive material, avirulence, biocompatibility is high, it is possible to realizes medicine and slowly discharges, either as medicine or health food,
The most nutritious but also healthy；The size of microcapsule is all at tens micron orders, powdered after lyophilizing, convenient application, easily preserves；And system
Standby technique is simple, substantially need not introduce poisonous and harmful chemical reagent, does not has the danger of dissolvent residual, be especially suitable for medicine and food
The embedding of additive；Yeast can make the waste yeast that fermentation plant reclaims simultaneously, wide material sources, cheap, easily biological-degradable,
It is suitable for industrialized great production.
Detailed description of the invention
Present disclosure is illustrated below by embodiment:
Embodiment 1
1) taking Carlina acaulis stem and leaf 2000g, Chinese medicine grinder is pulverized, and crosses 60 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 40
95% ethanol of times amount, 800W supersound extraction 3 times, each 180min；Filter, merging filtrate, be concentrated to give phase at 50 DEG C
Clear paste to density 1.25～1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast, add the 5%NaCl solution of 20 times amount, after 54 DEG C of water-baths vibration 5h, 5000rpm from
Heart 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
For 1:4 ratio mix, add the distilled water of 20 times amount, at 70 DEG C, water bath with thermostatic control vibration embedding 10h after, 5000rpm from
Heart 10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol.
The calculating microcapsule embedded rate of yeast: dried calculating embedding rate of poor quality before and after utilizing yeast cells to embed:
W%=(ma-mb)/ma*100%；In formula: W (%) is embedding rate, mb is the quality of the front yeast cells of embedding, after mb is for embedding
The yeast cells gross mass obtained.Being computed, the embedding rate of the yeast microcapsule in the present embodiment is 64.5%.
Embodiment 2
1) taking Carlina acaulis stem and leaf 1000g, Chinese medicine grinder is pulverized, and crosses 20 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 40
95% ethanol of times amount, 400W supersound extraction 3 times, each 120min；Filter, merging filtrate, be concentrated to give phase at 50 DEG C
Clear paste to density 1.25～1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast, add the 5%NaCl solution of 20 times amount, after 54 DEG C of water-baths vibration 5h, 5000rpm from
Heart 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
For 1:4 ratio mix, add the distilled water of 20 times amount, at 60 DEG C, water bath with thermostatic control vibration embedding 10h after, 5000rpm from
Heart 10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its
Embedding rate is 62.8%.
Embodiment 3
1) taking Carlina acaulis stem and leaf 1500g, Chinese medicine grinder is pulverized, and crosses 60 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 40
95% ethanol of times amount, 800W supersound extraction 3 times, each 180min；Filter, merging filtrate, be concentrated to give phase at 50 DEG C
Clear paste to density 1.25～1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast or the waste yeast of fermentation plant recovery, add the 5%NaCl solution of 20 times amount, 54 DEG C of water-baths
After vibration 5h, 5000rpm is centrifuged 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
Ratio for 1:1 mixes, and adds the distilled water of 20 times amount, and at 50 DEG C, after water bath with thermostatic control vibration embedding 4h, 5000rpm is centrifuged
10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its bag
The rate of burying is 30.8%.
Embodiment 4
1) taking Carlina acaulis stem and leaf 2000g, Chinese medicine grinder is pulverized, and crosses 40 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 40
60% ethanol of times amount, 800W supersound extraction 3 times, each 180min；Filter, merging filtrate, be concentrated to give phase at 50 DEG C
Clear paste to density 1.25～1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast, add the 5%NaCl solution of 20 times amount, after 54 DEG C of water-baths vibration 5h, 5000rpm from
Heart 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
Ratio for 1:4 mixes, and adds the distilled water of 20 times amount, and at 50 DEG C, after water bath with thermostatic control vibration embedding 4h, 5000rpm is centrifuged
10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its bag
The rate of burying is 58.7%.
Embodiment 5
1) taking Carlina acaulis stem and leaf 1000g, Chinese medicine grinder is pulverized, and crosses 50 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 30
75% ethanol of times amount, 400W supersound extraction 3 times, each 30min；Filter, merging filtrate, be concentrated to give at 50 DEG C relatively
The clear paste of density 1.25～1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast or the waste yeast of fermentation plant recovery, add the 5%NaCl solution of 20 times amount, 54 DEG C of water-baths
After vibration 5h, 5000rpm is centrifuged 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
For 1:3 ratio mix, add the distilled water of 20 times amount, at 40 DEG C, water bath with thermostatic control vibration embedding 10h after, 5000rpm from
Heart 10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its
Embedding rate is 48.9%.
Embodiment 6
1) taking Carlina acaulis stem and leaf 1000g, Chinese medicine grinder is pulverized, and crosses 30 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 40
80% ethanol of times amount, 600W supersound extraction 2 times, each 120min；Filter, merging filtrate, be concentrated to give phase at 50 DEG C
Clear paste to density 1.25～1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast, add the 5%NaCl solution of 20 times amount, after 54 DEG C of water-baths vibration 5h, 5000rpm from
Heart 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
Ratio for 1:4 mixes, and adds the distilled water of 20 times amount, and at 40 DEG C, after water bath with thermostatic control vibration embedding 5h, 5000rpm is centrifuged
10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its bag
The rate of burying is 59.7%.
Embodiment 7
1) taking Carlina acaulis stem and leaf 1500g, Chinese medicine grinder is pulverized, and crosses 60 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 40
60% ethanol of times amount, 700W supersound extraction 3 times, each 30min；Filter, merging filtrate, be concentrated to give at 50 DEG C relatively
The clear paste of density 1.25～1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast, add the 5%NaCl solution of 20 times amount, after 54 DEG C of water-baths vibration 5h, 5000rpm from
Heart 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
Ratio for 1:2 mixes, and adds the distilled water of 20 times amount, and at 40 DEG C, after water bath with thermostatic control vibration embedding 8h, 5000rpm is centrifuged
10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its bag
The rate of burying is 45.7%.
Embodiment 8
1) taking Carlina acaulis stem and leaf 2000g, Chinese medicine grinder is pulverized, and crosses 40 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 40
50% ethanol of times amount, 400W supersound extraction 30min；Filter, merging filtrate, be concentrated to give at 50 DEG C relative density 1.25～
The clear paste of 1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast, add the 5%NaCl solution of 20 times amount, after 54 DEG C of water-baths vibration 5h, 5000rpm from
Heart 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
Ratio for 1:4 mixes, and adds the distilled water of 20 times amount, and at 60 DEG C, after water bath with thermostatic control vibration embedding 4h, 5000rpm is centrifuged
10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its bag
The rate of burying is 60.3%.
Embodiment 9
1) taking Carlina acaulis stem and leaf 1000g, Chinese medicine grinder is pulverized, and crosses 40 mesh sieves, obtains Carlina acaulis stem and leaf powder, adds 40
75% ethanol of times amount, 500W supersound extraction 2 times, each 180min；Filter, merging filtrate, be concentrated to give phase at 50 DEG C
Clear paste to density 1.25～1.46, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take commercially available Angel dry yeast, add the 5%NaCl solution of 20 times amount, after 54 DEG C of water-baths vibration 5h, 5000rpm from
Heart 10min, washes 2 times, lyophilization, completes yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall ratio
For 1:23 ratio mix, add the distilled water of 20 times amount, at 40 DEG C, water bath with thermostatic control vibration embedding 7h after, 5000rpm from
Heart 10min, after distillation is washed 3 times and is repeatedly centrifuged, lyophilization, obtain the yeast microcapsule of described Carlina acaulis polyphenol, its
Embedding rate is 50.2%.
The above, only present pre-ferred embodiments, therefore the scope that the present invention implements can not be limited according to this, i.e. according to the present invention
The equivalence change that the scope of the claims and description are made with modify, all should still belong in the range of the present invention contains.
Claims (10)
1. the preparation method of the yeast microcapsule of a Carlina acaulis polyphenol, it is characterised in that: in described yeast microcapsule, yeast
Cell is embedding wall material, and Carlina acaulis polyphenol is core, and this preparation method includes:
1) take the Carlina acaulis stem and leaf powder that particle diameter is 10～60 mesh, add 35～98% ethanol of 8～45 times amount, 150～850
W supersound extraction 1～4 times, each 20～200min；Filter, merging filtrate, be concentrated to give at 50 DEG C relative density 1.23～
The clear paste of 1.48, lyophilization, prepare Carlina acaulis polyphenol lyophilized powder；
2) take dry yeast, add 4.5～5.5%NaCl solution of 18～22 times amount, 52～56 DEG C of water-bath vibrations 4.5～5.5h
After, 4000～6000rpm are centrifuged 8～12min, wash 1～3 time, lyophilization, complete yeast cells pretreatment；
3) by step 1) the Carlina acaulis polyphenol lyophilized powder that obtains and step 2) the pretreated yeast cells that obtains is according to core wall
Mix than the ratio for 1:0.2～5, add the water of 18～22 times amount, at 25～75 DEG C, water bath with thermostatic control vibration embedding 1～11h
After, 4000～6000rpm are centrifuged 8～12min, wash 2～4 times, lyophilization, obtain the ferment of described Carlina acaulis polyphenol
Female microcapsule, its embedding rate is 30～65%.
Preparation method the most according to claim 1, it is characterised in that: described step 1) in, add 39～41 times amount
94～96% ethanol, 780～820W supersound extraction 2～4 times, each 170～190min；Described step 3) in, core wall
9.5～10.5h are embedded than for 1:3.8～4.2,68～72 DEG C of water bath with thermostatic control vibrations；The microcapsule embedded rate of yeast obtained be 64～
65%.
Preparation method the most according to claim 1, it is characterised in that: described step 1) in, add 39～41 times amount
94～96% ethanol, 380～420W supersound extraction 2～4 times, each 110～130min；Described step 3) in, core wall
9.5～10.5h are embedded than for 1:3.8～4.2,58～62 DEG C of water bath with thermostatic control vibrations；The microcapsule embedded rate of yeast obtained be 62～
63%.
Preparation method the most according to claim 1, it is characterised in that: described step 1) in, add 39～41 times amount
59～61% ethanol, 780～820W supersound extraction 2～4 times, each 170～190min；Described step 3) in, core wall
3.5～4.5h are embedded than for 1:3.8～4.2,48～52 DEG C of water bath with thermostatic control vibrations；The microcapsule embedded rate of yeast obtained be 58～
59%.
Preparation method the most according to claim 1, it is characterised in that: described step 1) in, add 29～31 times amount
74～76% ethanol, 380～420W supersound extraction 2～4 times, each 25～35min；Described step 3) in, core wall ratio
For 1:2.8～3.2,38～42 DEG C of water bath with thermostatic control vibration embeddings 9.5～10.5h；The microcapsule embedded rate of yeast obtained be 48～
49%.
Preparation method the most according to claim 1, it is characterised in that: described step 1) in, add 39～41 times amount
79～81% ethanol, 580～620W supersound extraction 1～3 times, each 110～130min；Described step 3) in, core wall
4.5～5.5h are embedded than for 1:3.8～4.2,38～42 DEG C of water bath with thermostatic control vibrations；The microcapsule embedded rate of yeast obtained be 59～
60%.
Preparation method the most according to claim 1, it is characterised in that: described step 1) in, add 39～41 times amount
59～61% ethanol, 680～720W supersound extraction 2～4 times, each 25～35min；Described step 3) in, core wall ratio
For 1:1.8～2.2,48～52 DEG C of water bath with thermostatic control vibration embeddings 7.5～8.5h；The microcapsule embedded rate of yeast obtained be 45～
46%.
Preparation method the most according to claim 1, it is characterised in that: described step 1) in, add 39～41 times amount
49～51% ethanol, 380～420W supersound extraction 25～35min；Described step 3) in, core wall than for 1:3.8～4.2,
58～62 DEG C of water bath with thermostatic control vibration embeddings 3.5～4.5h；The microcapsule embedded rate of yeast obtained is 60～61%.
Preparation method the most according to claim 1, it is characterised in that: described step 1) in, add 39～41 times amount
74～76% ethanol, 480～520W supersound extraction 1～secondary, each 170～190min；Described step 3) in, core wall ratio
For 1:2.8～3.2,38～42 DEG C of water bath with thermostatic control vibration embeddings 6.5～7.5h；The microcapsule embedded rate of yeast obtained is 50～51%.
10. a yeast microcapsule for the Carlina acaulis polyphenol prepared by preparation method according to claim 1, its feature exists
In: in described yeast microcapsule, yeast cells is embedding wall material, and Carlina acaulis polyphenol is core, and its embedding rate is 30～65%.
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| CN111084373A (en) * | 2019-12-20 | 2020-05-01 | 吉林省桦树茸国际健康产业集团有限公司 | Extraction and microencapsulation of antioxidant component in Inonotus obliquus and application of antioxidant component in biscuit |
| CN110862903A (en) * | 2019-12-30 | 2020-03-06 | 江南大学 | Apple vinegar rich in lactic acid and succinic acid and production process thereof |
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