CN105920059A

CN105920059A - Chimonanthus praecox extract as well as preparation method and application thereof

Info

Publication number: CN105920059A
Application number: CN201610394829.3A
Authority: CN
Inventors: 朱剑虹; 王智富; 朱侗明
Original assignee: SHANGHAI SHENYIN BIOLOGICAL TECHNOLOGY Co Ltd
Current assignee: SHANGHAI SHENYIN BIOLOGICAL TECHNOLOGY Co Ltd
Priority date: 2016-06-06
Filing date: 2016-06-06
Publication date: 2016-09-07

Abstract

The invention discloses a chimonanthus praecox extract which is prepared from 5-20% of kaempferol, 10-30% of quercetin, 50-60% of scyllitol and inevitable impurities. The chimonanthus praecox extract is obtained by freeze-drying a chimonanthus praecox material by using liquid nitrogen, then grinding the chimonanthus praecox material into powder, adding an extracting agent into the powder, ultrasonically leaching an obtained mixture for 0.5-2h at 35-60 DEG C, carrying out centrifugation and filtration after terminating the leaching, collecting filtrate and carrying out chromatographic purification through a chromatographic column. The chimonanthus praecox extract provided by the invention can protect skin and reduce the disoperation by microorganisms, and has the functions of reducing ultraviolet radiation, resisting oxidation and resisting inflammations of the skin, so as to protect cells of the skin; the chimonanthus praecox extract is applied to cosmetics or health products, and can also reduce pigmentation, inhibit the generation of stains and enable the skin to be fair and bright.

Description

A kind of wax prunus mume extract, its preparation method and application

Technical field

The invention belongs to plant component and extract field, be specifically related to a kind of wax prunus mume extract, its preparation side Method and application.

Background technology

Wax prunus mume (sieb.) sieb.et zucc., has another name called chimonanthi,flos, fragrant prunus mume (sieb.) sieb.et zucc., originates in from China, grows thickly filling for Calycanthaceae wax-cakes bait fallen leaves Wood.Wax prunus mume (sieb.) sieb.et zucc. old origin, for Relict Plant in the Tertiary Period, one of Yi Shi China tradition famous flower.Wax prunus mume (sieb.) sieb.et zucc. makees For the traditional flowers of China, also it is one of significant flowers, is loved by the people from ancient times, in middle Chinese Why in change, the wax plum curculio of thirty years of age levies moral the cold winter, " cold to the bone without some, Flos Mume is assailed the nostrils perfume (or spice) " The most wide-spread Deng classical verse.

Flos Chimonanthi Praecocis was opened the cold early spring moon, first spent posterior lobe, and brightly yellowish as cured, delicate fragrance overflows, and views and admires for winter Good merchantable brand, is the distinctive precious ornamencal flower and tree of China.Wax definiteness happiness sunlight, the most resistance to half the moon, aversion to wind, relatively Cold-resistant, the energy safe overwintering when being not less than-15 DEG C, Beijing areas to the south can outdoor cropping.Wax prunus mume (sieb.) sieb.et zucc. is not only Being preferable ornamental flower, it is possible to do cut-flower, miniature gardening, flower can make tea, makes wine, is used as medicine, extract perfume Essence, its root, stem and leaf also has the effectiveness such as cough-relieving.

For a long time, sight is concentrated on wax prunus mume (sieb.) sieb.et zucc. as tea-drinking, essence, the purposes of Chinese medicine by people, right When wax prunus mume (sieb.) sieb.et zucc. is extracted, utilize ultrasound assisted extraction to take low frequency (10-20KHz) and intermediate frequency more (40kHz), when separating, eluant with the addition of alcohols solvent (such as butanediol-acetone system), Eluant system polarity is relatively big, and the component classification of extract is numerous and diverse and mostly is volatile material, extracts Thing composition instability, impact application effect, application is restricted.

And study and show, people are exposed in sunlight, atmosphere pollution for a long time, and body can produce too much Active oxygen and free radical, cause the Antioxidants of human body self to decline, cause cell viability Reducing, human body metabolic disorder, even infecting by harmful microorganism, finally makes skin accelerate Aging, the colour of skin is obscure, and acne is red and swollen, and application based on current wax prunus mume extract limits, by wax Prunus mume (sieb.) sieb.et zucc. is applied to the purposes of skin care after extracting, and there is not been reported.

Summary of the invention

It is an object of the invention to provide a kind of wax prunus mume extract, its preparation method and application, this extraction Thing can protect Skin Cell, has microorganism encroach, the ultraviolet radiation reducing skin and being suffered, Antioxidation etc. act on, it is also possible to play the effect of opposing scytitis, moreover it is possible to reduce pigmentation, press down Mottle processed generates, and it is vivid to make that skin and muscles are fair.

In order to achieve the above object, the technical scheme that the present invention provides is as follows:

A kind of wax prunus mume extract, its each weight percentages of components is: nimbecetin 5～20%, Quercetin 10～30%, Cocositol 50～60%, and inevitably impurity.

The preparation method of wax prunus mume extract of the present invention, comprises the following steps:

1) by wax prunus mume (sieb.) sieb.et zucc. material clean, shred, sterilize；

2) by the wax prunus mume (sieb.) sieb.et zucc. material liquid nitrogen lyophilizing after sterilization, pulverizing, extractant is added, at airtight condition Under, ultrasonic extraction 0.5～2h, extraction temperature is 35～60 DEG C, supersonic frequency 60-100kHz；Wherein, The mass volume ratio of wax prunus mume (sieb.) sieb.et zucc. and extractant is 1:30～60；

3) it is centrifuged after extraction terminates, filters, collect filtrate, obtain the solution of wax prunus mume extract；

4) by step 3) solution of wax prunus mume extract that obtains, concentrate, utilize chromatographic column to chromatograph Purification, gradient elution, eluant is petroleum ether-acetone；

5) collect eluent, dry, obtain described wax prunus mume (sieb.) sieb.et zucc. branch extract.

Further, step 2) in, step 2) described in extractant selected from 70～90% water of ethanol At least one in the aqueous solution of solution, ethyl acetate and saturated n-butyl alcohol.

Preferably, step 2) in, the mass ratio of wax prunus mume (sieb.) sieb.et zucc. material and extractant is 1:35～45.

The most preferably, described step 2) carry out under anaerobic.

Further, step 4) in, when utilizing column chromatography purification, with silica column purification 2～5 times, Purify 2～5 times with hydroxypropyl dextran gel column chromatography, collect petroleum ether and acetone volume in eluant Than the eluent when 100:6～12.

Further, described wax prunus mume (sieb.) sieb.et zucc. material is the branch of wax prunus mume (sieb.) sieb.et zucc. plant, stem, leaf or flower.

The present invention provides a kind of compositions comprising described wax prunus mume extract, including: glycerol 2～7wt.%, Butanediol 3～5wt.%, 1,2-pentanediol 1～5wt.%, EDETATE SODIUM 0.1～0.4wt.%, glycerol Stearate 1～3wt.%, hyaluronate sodium 0～0.1wt.%, xanthan gum 1～10wt.%, wax prunus mume (sieb.) sieb.et zucc. carry Taking thing 2～8wt.%, surplus is water.

The wax prunus mume extract of the present invention can be applicable in the preparation of cosmetics or health product, described cosmetic Product preferred whitening class, anti-acne and/or antibacterial class cosmetics.

When wax prunus mume (sieb.) sieb.et zucc. material is extracted by the present invention, supersonic frequency is 60-100kHz, and higher is ultrasonic Frequency can add the dissolution of fast component, and can improve the dissolubility of solute, carries out wax prunus mume extract During column chromatography purification, use petroleum ether-acetone eluant system, gradient elution, obtain petroleum ether and third The volume ratio of the ketone eluent when 100:6～12, the wax prunus mume extract obtained contains abundant Rhizoma Kaempferiae Phenol, Quercetin and Cocositol.

The wax prunus mume extract of the present invention contains abundant nimbecetin, Quercetin and Cocositol, it is possible to protection The effects such as skin reduces microorganism encroach, has minimizing ultraviolet radiation, antioxidation, thus protect skin Skin cell, wherein nimbecetin and Quercetin can also play opposing scytitis effect, meanwhile, Can also scavenging activated oxygen, suppress tyrosinase activity, and then inhibit mottle to generate, make skin white Fair-skinned vivid.

Containing nimbecetin in the wax prunus mume extract of the present invention, mass percent is 5%～20%, nimbecetin Having non-oxidizability, nimbecetin demonstrates the array with antioxidation in vitro and in vivo, During low concentration, superoxides scavenger can be served as, particularly to the hydroxyl radical free radical of high response and Peroxynitrite species, in high concentration, it increases antioxidase such as superoxide dismutase, mistake Hydrogen oxide enzyme, and the activity of Heme oxygenases-1 or expression, and the microorganisms such as antibacterial can be suppressed Growth, for skin provide multiple protective.

Containing Quercetin in the wax prunus mume extract of the present invention, mass percent is: 10%～30%, its Content in wax prunus mume (sieb.) sieb.et zucc. branch is the abundantest, and Quercetin has the resistance promoting capillary of skin, alleviates Sensitive skin, it helps the growth of Skin Cell and vigor keep.

Containing Cocositol in the wax prunus mume extract of the present invention, mass percent is 50%～60%, shark flesh Alcohol can disconnect ester bond, decomposes cholesterol and the granule of fat Cheng Geng little, reduces skin surface lipid heap Long-pending, reduce the generation of skin fat grain, make skin fine and smooth, promote skin vitality, improve skin physiology State.

Beneficial effects of the present invention:

The present invention, by extracting isolated wax prunus mume (sieb.) sieb.et zucc. branch extract, organically combines wax prunus mume (sieb.) sieb.et zucc. institute rich The three kinds of composition nimbecetins contained, Quercetin and Cocositol, these three composition cooperates, Quercetin with Nimbecetin has antibacterial effect, and the cleaning of ester matter can be destroyed the growth ring of pathogenic bacteria by Cocositol Border, strengthens bacteriostasis；Nimbecetin can protect vascular endothelial growth with scavenging activated oxygen Microenvironment, is more beneficial for the blood capillary of Quercetin protection skin, and therefore, three kinds of materials present collaborative Relation, it is possible to obtain more preferably effect.

Accompanying drawing explanation

Fig. 1 is the standard curve of hyaluronic acid in the embodiment of the present invention 2.

Fig. 2 is the increased percentage of hyaluronic acid in the embodiment of the present invention 2.

Fig. 3 is the survival rate change of skin flbroblast in the embodiment of the present invention 3.

Detailed description of the invention

Below in conjunction with the drawings and specific embodiments, the invention will be further described.

Embodiment 1

Utilize wax prunus mume (sieb.) sieb.et zucc. branch to prepare wax prunus mume extract, comprise the following steps:

1) 50g wax prunus mume (sieb.) sieb.et zucc. branch is cleaned, shreds, sterilizes；

2) by the wax prunus mume (sieb.) sieb.et zucc. branch liquid nitrogen lyophilizing after sterilization, pulverizing, 2L ethanol is added, at airtight bar Under part, temperature is 50 DEG C, ultrasonic extraction 1h, supersonic frequency 80kHz；

3) it is centrifuged after extraction terminates, filters, collect and obtain 500mL filtrate；

4) by step 3) filtrate concentrate after, utilize chromatographic column to be purified, cross 200～300 mesh silicon Glue chromatographic column 2～5 times, hydroxypropyl dextran gel column chromatography 2～5 times, eluant is petroleum ether-the third The volume ratio of ketone, gradient elution, petroleum ether and acetone (collects petroleum ether from 100:0 to 100:14 It is the product of 100:6,100:8,100:10 and 100:12 with acetone volume ratio).

5) collect eluent, concentrate, dry, obtain 0.8g extract.

In the present embodiment, by the adjustment number of times of chromatography concentrated filtrate, obtain different containing Amount combination, such as:

Wax prunus mume extract 1, excessively silica gel chromatographic column 3 times, hydroxypropyl dextran gel column chromatography 3 times: Nimbecetin (84mg/g), Quercetin (120mg/g), Cocositol (537mg/g)；

Wax prunus mume extract 2, excessively silica gel chromatographic column 4 times, hydroxypropyl dextran gel column chromatography 4 times: Nimbecetin (61mg/g), Quercetin (280mg/g), Cocositol (508mg/g)；

Wax prunus mume extract 3, excessively silica gel chromatographic column 2 times, hydroxypropyl dextran gel column chromatography 2 times: Nimbecetin (150mg/g), Quercetin (192mg/g), Cocositol (588mg/g).

Embodiment 2 uses wax prunus mume extract to verify the bacteriostasis of microbial infection

Hyaluronic acid is by fibroblasts to secrete, and the secretory volume of hyaluronic acid can embody skin Fibrocyte is to by bacteriostasis during microbial infection.

1. prepare the Skin Cell of people

Take the dermal fibroblast of normal person as primary cell, in 75cm²Culture bottle (contains Culture medium 2ml of somatomedin), 37 DEG C, CO₂Concentration is to cultivate cell under conditions of 5% to 80% Concentration, passes on, passage cell continue in 175cm2 square vase cultivate cell to 80% concentration, Obtain fibroblasts of adult human dermis.

People obtained above is cultivated true with the DMEM culture fluid containing volume fraction 10% hyclone Skin fibroblast, in 37 DEG C, 5%CO₂Cultivate 24 hours, treat that cell is close and merge, with containing 0.25% Pass on by 1:2 after the Digestive system digestion of trypsin and 0.02%EDTA, choose forth generation cell.

With the DMEM culture fluid containing 10% hyclone, forth generation cell being diluted to density is 10⁵ Individual cell/ml, inoculates in 12 orifice plates, and inoculum concentration is 2 × 10³Individual cells/well, cultivates to cell After merging close to 80%, wash plate 3 times with normal saline, obtain being vaccinated with fibroblasts of adult human dermis Orifice plate.

2. prepare to treat test agent

Preparation wax prunus mume extract essence, essence composition: glycerol 5.0wt.%, butanediol 4.0wt.%, 1,2-pentanediol 3.0wt.%, EDETATE SODIUM 0.1wt.%, glyceryl stearate 1.5wt.%, transparent Matter acid sodium 0.05wt.%, xanthan gum 4.0wt.%, wax prunus mume extract 3 5.0wt.% of embodiment 1, Surplus is deionized water.

Propionibacterium acnes is coated in LB culture medium, 37 DEG C of incubators are cultivated 48h, meter The bacterial population obtained, and be inoculated in above-mentioned steps 1 by each culture hole 500 bacterial population) in obtain 12 orifice plates being vaccinated with fibroblasts of adult human dermis in, choose 15 in two 12 orifice plates thin The hole of bacterium inoculation, wherein 5 Kong Weiyi groups, if 3 groups of parallel controls, in 5 holes often organized respectively Add A sample (essence 0.01mL+ normal saline 0.29mL), B sample (essence 0.05mL + normal saline 0.25mL), C sample (essence 0.1mL+ normal saline 0.2mL), D sample (essence 0.3mL) and control sample (0.3mL normal saline), at 37 DEG C, 5%CO₂ Under the conditions of cultivate 12h, collect A sample, B sample, C sample, D sample and comparison the most respectively The culture supernatant of group sample is standby, containing being infected by propionibacterium acnes in this culture supernatant Fibroblasts of adult human dermis.

3. draw hyaluronic acid standard curve

Preparing 6 hyaluronic acid standard solutions, concentration is followed successively by: 0 μ g/mL, 10 μ g/mL, 20 μ g/mL, 40 μ g/mL, 80 μ g/mL, 160 μ g/mL, utilize microplate reader to test at 450 nm Its OD value, test result sees table 1.

Table 1

According to people's hyaluronic acid enzyme immunoassay test kit (Mei Lian bio tech ltd, Shanghai) Description, according to hyaluronic acid standard substance test result, with OD value as the longitudinal axis, dense with hyaluronic acid Spend the standard curve drawing hyaluronic acid for transverse axis, see Fig. 1；OD=0.0171 × c+0.2857, Coefficient R²=0.977, p=0.004, wherein, c is the concentration of hyaluronic acid, unit μ g/mL, Through converting, hyaluronic acid concentration c=(OD-0.2857)/0.0171.

4. carry out hyaluronic acid contents mensuration

According to people's hyaluronic acid enzyme immunoassay test kit description, employment hyaluronic acid enzyme linked immunological Solvent in assay kit is by the A sample got ready in step 2, B sample, C sample, D sample Dilute 5 times respectively with the culture supernatant of control sample, measure the A sample after dilution 5 times, B The OD value of the culture supernatant of sample, C sample, D sample and control sample, further according to transparent The standard curve of matter acid calculates hyaluronic acid contents, and result sees table 2 and Fig. 2.

Table 2

	Matched group	B sample	C sample	D sample
					Dilute 5 times and record OD (450nm)	0.48	0.63	0.81	0.56
Hyaluronic acid concentration c (μ g/ml)	56.81	100.67	153.30	80.20
					Content is (%) compared with matched group	0.0%	77.2%	169.9%	41.2%

By table 2 and Fig. 2 it can be seen that add the essence of the wax prunus mume (sieb.) sieb.et zucc. branch extract preparation of the present invention After, fibroblasts of adult human dermis content of secretion hyaluronic acid under the infecting of propionibacterium acnes has aobvious Writing and improve, hyaluronic acid is reflected by fibroblasts to secrete, the increase of hyaluronic acid secretion amount This wax prunus mume (sieb.) sieb.et zucc. branch extract contributes to skin flbroblast and supports antimicrobial infecting.

Embodiment 3 verifies the resistance to pathogenic microorganism of wax prunus mume extract

1. collect cell

Collect A sample, B sample, C sample, D sample and comparison in step 2 in embodiment 2 Fibroblasts of adult human dermis in the culture supernatant of group sample, in rotating speed 500～1000r/min condition Under be centrifuged 5min, take lower sediment, i.e. fibroblasts of adult human dermis, wash with 1mlPBS buffer Wash 1 time, then be centrifuged, discard PBS, the ethanol of the 70% of addition ice pre-cooling, be fixed, 4 DEG C, 1～2 hour, the centrifugal fixative that discards, resuspended 5 minutes of addition 3mLPBS buffer.

2. cell filtration

With 400 eye mesh screens the fibroblasts of adult human dermis that obtains filtered 1 time, 500～1000r/min Centrifugal 5min, discards buffer.

3. dyeing

Dye with 1ml PI dye liquor, be positioned in 4 DEG C of refrigerators, lucifuge 30 minutes.

4. flow cytomery

By the survival condition of the fibroblasts of adult human dermis that flow cytomery obtains, measure respectively The survival number of fibroblasts of adult human dermis, apoptosis number, calculate survival rate, and result sees table 3.

Table 3

	Matched group	A sample	B sample	C sample	D sample
						Skin Cell survival number (10³)	0.4	0.4	1.2	1.9	0.6
Skin Cell apoptosis number (10³)	2.7	2.6	1.8	1.2	2.6
						Skin Cell survival rate (%)	12.90	13.33	40.00	61.29	18.75

Propionibacterium acnes can affect the growth of fibroblasts of adult human dermis, as can be seen from Table 3, adds After entering the wax prunus mume extract of the present invention, skin flbroblast, under the competition of propionibacterium acnes, is deposited Motility rate is significantly increased, and illustrates that this essence contributes to fibroblasts of adult human dermis to pathogenic microorganism Resistance.

Embodiment 4 verifies the effect of the scavenging activated oxygen of wax prunus mume extract

Under the effect of tryrosinase, levodopa (L-DOPA) is oxidized to tan DOPA color Element, under the existence condition of inhibitor, this reaction will be suppressed, the wax prunus mume (sieb.) sieb.et zucc. branch of the test present invention The extract suppression ratio to tyrosinase activity, comprises the steps:

A sample, C sample, D sample and the matched group that the embodiment of the present invention 2 second step is obtained The each sample of culture supernatant of sample respectively takes 0.1ml, is separately added in advance in 30 DEG C of waters bath with thermostatic control In the test tube of the L-DOPA substrate solution of the 2.8ml of insulation, mixing, it is subsequently adding the 600 of 0.1ml Carry out water-bath 10 minutes in 30 DEG C after the tyrosinase solution of unit/ml, fully mixing, use light splitting Photometer, measures the absorption peak of dopachrome at 475nm.

By the absorbance measured, calculate the dilution of stock suppression ratio (%) to tyrosinase activity, Seeing table 4, wherein, the computing formula of suppression ratio is as follows:

Suppression ratio=[1-(T1-T2)/(C1-C2)] × 100%

In above-mentioned computing formula,

C1 adds the mixture of tryrosinase and is surveyed by not adding sample (A sample, C sample, D sample) Absorbance；

C2 does not adds the mixture of tryrosinase for not adding sample (A sample, C sample, D sample) yet The absorbance surveyed；

T1 is surveyed by adding sample (A sample, C sample, D sample) and the mixture of tryrosinase Absorbance；

T2 does not adds the mixture institute of tryrosinase for adding sample (A sample, C sample, D sample) The absorbance surveyed.

Table 4

Sample	C1	C2	T1	T2	Suppression ratio (%)
						A sample	0.65	0.31	0.63	0.29	0.11
C sample	0.67	0.3	0.62	0.32	18.05
						D sample	0.62	0.28	0.46	0.27	44.1
Matched group	0.68	0.33	0.61	0.26	0

As can be seen from Table 5, the wax prunus mume (sieb.) sieb.et zucc. branch extract obtained by the present invention is for the work of tryrosinase Property have obvious inhibitory action, along with concentration raise, inhibitory action strengthen, this can suppress mottle Formed, play the effect making skin-whitening.

The application verification of the wax prunus mume (sieb.) sieb.et zucc. branch compositions of embodiment 5 present invention

Cleaner arm, by the A sample described in the embodiment of the present invention 2 second step, C sample, D Sample is respectively coated the skin of 2.5cm × 2.5cm on arm, and is coated with normal saline for control sample In equal area, respectively 1 hour, 2 hours, observe skin condition after 5 hours and carry out record, The results are shown in Table 5.

Table 5

Test period	A sample	C sample	D sample	Matched group
					1 hour	Moistening	Moistening	Moistening, pale	Moistening
2 hours	Greasy	Normally	Moistening, pale	The driest
					5 hours	Greasy	Normally	Normally	Greasy

As can be seen from Table 5, after adding the wax prunus mume (sieb.) sieb.et zucc. branch extract of the present invention, the water holding capacity of skin Increasing, whitening skin and preserving moisture effect is obvious.

The wax prunus mume extract of the present invention can protect skin to reduce microorganism encroach, has minimizing ultraviolet Radiation, antioxidation etc. act on, thus protect Skin Cell, this wax prunus mume extract can also play to The effect of anti-scytitis, meanwhile can also reduce pigmentation, it is suppressed that mottle generates, and makes flesh Skin is pale vivid.

Claims

1. a wax prunus mume extract, its each weight percentages of components is: nimbecetin 5～20%, Quercetin 10～30%, Cocositol 50～60%, and inevitably impurity.

2. the preparation method of wax prunus mume extract as claimed in claim 1, comprises the following steps:

1) by wax prunus mume (sieb.) sieb.et zucc. material clean, shred, sterilize；

2) by the wax prunus mume (sieb.) sieb.et zucc. material liquid nitrogen lyophilizing after sterilization, pulverizing, extractant is added, at airtight condition Under, ultrasonic extraction 0.5～2h, extraction temperature is 35～60 DEG C, supersonic frequency 60-100kHz； Wherein, the mass volume ratio of wax prunus mume (sieb.) sieb.et zucc. and extractant is 1:30～60；

3. the preparation method of wax prunus mume extract as claimed in claim 2, it is characterised in that step 2) in, Step 2) described in extractant selected from 70～90% aqueous solution of ethanol, ethyl acetate and saturated At least one in the aqueous solution of n-butyl alcohol.

4. the preparation method of wax prunus mume extract as claimed in claim 2 or claim 3, it is characterised in that step 2) In, the mass ratio of wax prunus mume (sieb.) sieb.et zucc. material and extractant is 1:35～45.

5. the preparation method of wax prunus mume extract as claimed in claim 3, it is characterised in that described step Rapid 2) carry out under anaerobic.

6. the preparation method of wax prunus mume extract as claimed in claim 2, it is characterised in that step 4) in, When utilizing column chromatography purification, with silica column purification 2～5 times, use hydroxypropyl polydextran gel Column chromatography purification 2～5 times, collect in eluant petroleum ether and acetone volume ratio at 100:6～12 Time eluent.

7. the preparation method of the wax prunus mume extract as described in any one of claim 3～6, it is characterised in that Described wax prunus mume (sieb.) sieb.et zucc. material is the branch of wax prunus mume (sieb.) sieb.et zucc. plant, stem, leaf or flower.

8. the compositions containing wax prunus mume extract described in claim 1, including: glycerol 2～7wt.%, Butanediol 3～5wt.%, 1,2-pentanediol 1～5wt.%, EDETATE SODIUM 0.1～0.4wt.%, sweet Oleostearin acid esters 1～3wt.%, hyaluronate sodium 0～0.1wt.%, xanthan gum 1～10wt.%, wax Prunus mume extract 2～8wt.%, surplus is water.

9. wax prunus mume extract application in cosmetics or health product as claimed in claim 1.

Apply the most as claimed in claim 9, it is characterised in that described cosmetics are whitening class, dispel Pox and/or antibacterial class cosmetics.