CN105920037A - Application of nano activated carbon sol in preparation of herpes virus therapeutics - Google Patents
Application of nano activated carbon sol in preparation of herpes virus therapeutics Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/44—Elemental carbon, e.g. charcoal, carbon black
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
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Abstract
The invention discloses application of nano activated carbon sol in preparation of herpes virus therapeutics. The nano activated carbon sol is 1-100 nm in activated carbon particle size. The mass concentration of nano activated carbon in the nano carbon sol is less than or equal to 10%., and the surface of the nano activated carbon is modified with carboxyl groups. The application of the nano activated carbon sol in the preparation of therapeutics for treating skin diseases due to herpes virus provides significant effect, short process, high acting speed and zero recurrence after healing, great advance is made in medical science, and great clinical application value and practical significance are provided.
Description
Technical field
The present invention relates to active carbon nanoparticles applied technical field, be specifically related to the application in preparation treatment herpesvirus medicament of a kind of active carbon nanoparticles colloidal sol.
Background technology
Herpesvirus (Herpesviruses) is a group medium sized distrand DNA virus, is divided into tri-subfamilies of α, β, γ according to its physicochemical property.α herpesvirus (such as herpes simplex virus, chickenpox one varicella zoster virus) growth rate is fast, causes cytopathy.β herpesvirus (such as cytomegalovirus), growth cycle is long, and infection cell forms giant cell.Gamma herpes viruses (such as Epstein-Barr virus), the target cell of infection is lymphoid cell, can cause lymphocytic hyperplasia.The host range of herpesvirus infection is extensive, can infect the mankind and other vertebratess.Cause what the mankind produced to have 8 kinds of herpesviruss, it may be assumed that herpes simplex virus type 1 (nerpes vinrus hominis 1 type), herpes simplex virus type 2 (nerpes vinrus hominis 2 type), varicella zoster virus (nerpes vinrus hominis 3 type), Epstein-Barr virus (ebb virus), cytomegalovirus (human herpes virus-5), human herpes virus-6, HHV-7, human herpes virus type 8.
Metainfective common presentation is: neuroganglion body of gland, kidney lymphoid tissue, lymphoid tissue herpes febrilis；Lip, eye, brain infect；Genital herpes chickenpox；Herpes zoster monocytosis, eye, kidney, brain and congenital infection infectious monocytosis, Burkitt lymphoma, nasopharyngeal carcinoma, baby's urgency rash and some other such as diseases such as unknown stomachache.
Current Therapeutic Principle's one makes the HSV of infection not activate, even elimination virus；Its two regulations immunity, prevents from sending out again, and available acyclovir quiet or oral, beautiful pearl prestige is administered orally, interferon intramuscular injection, interleukin II intramuscular injection.Acyclovir (ACV): be the choice drug generally acknowledged at present.Additionally also have interferon (IFN), virus (ribavirin), foscarnet sodium etc..But primary disease has self limiting, about 1-2 week gets final product spontaneous recovery.The purpose of Drug therapy is to prevent recurrence next time.Primary disease there is no specific medicament at present, and Therapeutic Principle, for shortening the course of disease, prevents secondary infection, reduces recurrence.
Summary of the invention
Present invention solves the technical problem that and be, overcome the defect of prior art, it is provided that the application in preparation treatment herpesvirus medicament of a kind of active carbon nanoparticles colloidal sol, effectively treat herpesvirus, short treating period, curative effect are fast, the recurrence after healing can be avoided.
The technical scheme is that the application in preparation treatment herpesvirus medicament of a kind of active carbon nanoparticles colloidal sol.
Further, in such scheme, the activated carbon particle size of described active carbon nanoparticles colloidal sol is 1-100nm.
Further, in such scheme, in described nano carbon sol, the mass concentration of active carbon nanoparticles is less than or equal to 10 ‰.
Further, in such scheme, the surface of described active carbon nanoparticles is modified with carboxyl functional group.
Further, the preparation method surface of described active carbon nanoparticles being modified carboxyl functional group is:
Step (1), dispersion active carbon nanoparticles: deionized water and amino benzoic Acid will be added in active carbon nanoparticles, the consumption of described amino benzoic Acid accounts for the 6～15% of described active carbon nanoparticles quality, carry out supersound process, obtain the finely dispersed active carbon nanoparticles suspension that concentration is 0.3g/L～0.8g/L；
Step (2), make to have on scattered nano active carbon surface carboxyl functional group: scattered active carbon nanoparticles suspension strong acid adjustment PH to 5.5～6.0 is obtained acidic carboxypolymer active carbon nanoparticles turbid solution, PH to 6～7 is filtered in the deionized water drip washing of solid matter in acidic carboxypolymer active carbon nanoparticles turbid solution, obtains aqueous carboxylated active carbon nanoparticles；By carboxylated for water active carbon nanoparticles lyophilization, cryodesiccated temperature is-20 DEG C, and drying time is 24～36h, i.e. obtains the active carbon nanoparticles on surface with carboxyl functional group.
Further, in described step (1), the power of supersound process is 800W～850W, and ultrasonic time is 15min～20min, and controlling temperature range is 50～55 DEG C.
Further, in step (2), described strong acid is hydrochloric acid or sulphuric acid.
The invention has the beneficial effects as follows: being used for active carbon nanoparticles colloidal sol preparing in the dermatosis medicine that treatment herpesvirus causes, effect is notable, short treating period, curative effect is fast, the recurrence after healing can be avoided, medically made significant headway, there is the biggest clinical value and practical significance.
Detailed description of the invention
Embodiment 1:
The application in preparation treatment herpesvirus medicament of a kind of active carbon nanoparticles colloidal sol.The activated carbon particle size of described active carbon nanoparticles colloidal sol is 1nm.In described nano carbon sol, the mass concentration of active carbon nanoparticles is for for 2 ‰.It is modified with carboxyl functional group on the surface of described active carbon nanoparticles.
Wherein, the preparation method surface of described active carbon nanoparticles being modified carboxyl functional group is:
Step (1), dispersion active carbon nanoparticles: deionized water and amino benzoic Acid will be added in active carbon nanoparticles, the consumption of described amino benzoic Acid accounts for the 6% of described active carbon nanoparticles quality, carry out supersound process, the power of supersound process is 800W, ultrasonic time is 15min, and controlling temperature range is 50 DEG C, obtains the finely dispersed active carbon nanoparticles suspension that concentration is 0.3g/L；
Step (2), make to have on scattered nano active carbon surface carboxyl functional group: scattered active carbon nanoparticles suspension strong acid adjustment PH to 5.5 is obtained acidic carboxypolymer active carbon nanoparticles turbid solution, described strong acid is hydrochloric acid or sulphuric acid, PH to 6 is filtered in the deionized water drip washing of solid matter in acidic carboxypolymer active carbon nanoparticles turbid solution, obtains aqueous carboxylated active carbon nanoparticles；By carboxylated for water active carbon nanoparticles lyophilization, cryodesiccated temperature is-20 DEG C, and drying time is 24h, i.e. obtains the active carbon nanoparticles on surface with carboxyl functional group.
Embodiment 2:
The application in preparation treatment herpesvirus medicament of a kind of active carbon nanoparticles colloidal sol.The activated carbon particle size of described active carbon nanoparticles colloidal sol is 50nm.In described nano carbon sol, the mass concentration of active carbon nanoparticles is for for 6 ‰.It is modified with carboxyl functional group on the surface of described active carbon nanoparticles.
Wherein, the preparation method surface of described active carbon nanoparticles being modified carboxyl functional group is:
Step (1), dispersion active carbon nanoparticles: deionized water and amino benzoic Acid will be added in active carbon nanoparticles, the consumption of described amino benzoic Acid accounts for the 10.5% of described active carbon nanoparticles quality, carry out supersound process, the power of supersound process is 825W, ultrasonic time is 17.5min, and controlling temperature range is 52.5 DEG C, obtains the finely dispersed active carbon nanoparticles suspension that concentration is 0.55g/L；
Step (2), make to have on scattered nano active carbon surface carboxyl functional group: scattered active carbon nanoparticles suspension strong acid adjustment PH to 5.8 is obtained acidic carboxypolymer active carbon nanoparticles turbid solution, described strong acid is hydrochloric acid or sulphuric acid, PH to 6.5 is filtered in the deionized water drip washing of solid matter in acidic carboxypolymer active carbon nanoparticles turbid solution, obtains aqueous carboxylated active carbon nanoparticles；By carboxylated for water active carbon nanoparticles lyophilization, cryodesiccated temperature is-20 DEG C, and drying time is 30h, i.e. obtains the active carbon nanoparticles on surface with carboxyl functional group.
Embodiment 3:
The application in preparation treatment herpesvirus medicament of a kind of active carbon nanoparticles colloidal sol.The activated carbon particle size of described active carbon nanoparticles colloidal sol is 100nm.In described nano carbon sol, the mass concentration of active carbon nanoparticles is for for 10 ‰.It is modified with carboxyl functional group on the surface of described active carbon nanoparticles.
Wherein, the preparation method surface of described active carbon nanoparticles being modified carboxyl functional group is:
Step (1), dispersion active carbon nanoparticles: deionized water and amino benzoic Acid will be added in active carbon nanoparticles, the consumption of described amino benzoic Acid accounts for the 15% of described active carbon nanoparticles quality, carry out supersound process, the power of supersound process is 850W, ultrasonic time is 20min, and controlling temperature range is 55 DEG C, obtains the finely dispersed active carbon nanoparticles suspension that concentration is 0.8g/L；
Step (2), make to have on scattered nano active carbon surface carboxyl functional group: scattered active carbon nanoparticles suspension strong acid adjustment PH to 6.0 is obtained acidic carboxypolymer active carbon nanoparticles turbid solution, described strong acid is hydrochloric acid or sulphuric acid, PH to 7 is filtered in the deionized water drip washing of solid matter in acidic carboxypolymer active carbon nanoparticles turbid solution, obtains aqueous carboxylated active carbon nanoparticles；By carboxylated for water active carbon nanoparticles lyophilization, cryodesiccated temperature is-20 DEG C, and drying time is 36h, i.e. obtains the active carbon nanoparticles on surface with carboxyl functional group.
Anti-herpesvirus experiment effect is studied:
1, object of study: age-3 years old June 30, female adult health tree；
2, object of study pretreatment:
Being fixed in special beanbag by tree, weigh and record its body weight, at tree femoribus internus muscle abundant place injection ketalar, the consumption of ketamine (ketamine) is for being 50mg/kg by tree body weight, and after 5 minutes, tree is in narcotism；
3, virus infects:
Tree back shaves out the region of 3*3cm size, and surface scratch, the most each cut ten times, is 2 × 10 with liquid-transfering gun by dosage4PFU/ herpes simplex virus only is added drop-wise to the cut district of tree respectively, i.e. can get the tree animal of herpes simplex infection；After Infection Action terminates, animal is put back to animal feeding room, treat that animal revives naturally, after tree infects in 1～2 week, clinical symptoms and the infection conditions of animal is once set in observation in every 12 hours, the survival rate of statistics animal, finds: tree during HSV-1 actute infection, occur by hair slightly unrest, anorexia, lose weight, drowsiness, the symptom such as lack cordiality.
Randomly select 15 to set as experimental group, set animal as a control group by other 15；
4, Therapeutic Method:
Experimental group: the active carbon nanoparticles colloidal sol of the application embodiment of the present invention 2 preparation is treated；
Matched group: application antiviral drugs Acyclovir is treated.
5, therapeutic outcome: experimental group 15 tree is after the active carbon nanoparticles colloidal sol of application invention embodiment 2 preparation carries out treating 3, all symptoms have alleviated, during continual cure to the 5th day, and major part transference cure, during to the 7th day, the most all trees return to one's perfect health.And matched group 15 tree is after application antiviral drugs Acyclovir carries out treating 3, only 2 tree symptoms have been alleviated, during continual cure to the 5th day, 6 tree symptoms are had to alleviate, during to the 7th day, having 8 tree partial symptoms and be eased, none is only cured.
6, conclusion: visible by above experiment, active carbon nanoparticles colloidal sol of the present invention is evident in efficacy in the dermatosis that treatment simplex keratitis causes, the common antiviral drugs better efficacy of ratio, onset more block, misery can be alleviated to a certain extent, make the life better quality, there is rapid healing, shorten the feature of the course of disease.
Obviously, the above embodiment of the present invention is only for clearly demonstrating example of the present invention, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here without also cannot all of embodiment be given exhaustive.And these spirit belonging to the present invention are extended out obvious change or variation still in protection scope of the present invention among.
Claims (7)
1. the application treated in herpesvirus medicament prepared by an active carbon nanoparticles colloidal sol.
Apply the most as claimed in claim 1, it is characterised in that the activated carbon particle size of described active carbon nanoparticles colloidal sol is 1-100nm.
3. the application as described in claim 1 alive 2, it is characterised in that in described nano carbon sol, the mass concentration of active carbon nanoparticles is less than or equal to 10 ‰.
Apply the most as claimed in claim 1 or 2, it is characterised in that on the surface of described active carbon nanoparticles, be modified with carboxyl functional group.
Apply the most as claimed in claim 4, it is characterised in that the preparation method modifying carboxyl functional group on the surface of described active carbon nanoparticles is:
Step (1), dispersion active carbon nanoparticles: deionized water and amino benzoic Acid will be added in active carbon nanoparticles, the consumption of described amino benzoic Acid accounts for the 6～15% of described active carbon nanoparticles quality, carry out supersound process, obtain the finely dispersed active carbon nanoparticles suspension that concentration is 0.3g/～0.8g/L；
Step (2), make to have on scattered nano active carbon surface carboxyl functional group: scattered active carbon nanoparticles suspension strong acid adjustment PH to 5.5～6.0 is obtained acidic carboxypolymer active carbon nanoparticles turbid solution, PH to 6～7 is filtered in the deionized water drip washing of solid matter in acidic carboxypolymer active carbon nanoparticles turbid solution, obtains aqueous carboxylated active carbon nanoparticles；By carboxylated for water active carbon nanoparticles lyophilization, i.e. obtain the active carbon nanoparticles on surface with carboxyl functional group.
Applying the most as claimed in claim 5, it is characterised in that in described step (1), the power of supersound process is 800W～850W, and ultrasonic time is 15min～20min, and controlling temperature range is 50～55 DEG C.
Applying the most as claimed in claim 6, it is characterised in that in step (2), described strong acid is hydrochloric acid or sulphuric acid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020210376A1 (en) * | 2019-04-09 | 2020-10-15 | The Board Of Trustees Of The University Of Illinois | Drug adsorbed highly porous activated carbon for enhanced drug delivery |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020210376A1 (en) * | 2019-04-09 | 2020-10-15 | The Board Of Trustees Of The University Of Illinois | Drug adsorbed highly porous activated carbon for enhanced drug delivery |
| US20220160872A1 (en) * | 2019-04-09 | 2022-05-26 | The Board Of Trustees Of The University Of Illinois | Drug Adsorbed Highly Porous Activated Carbon for Enhanced Drug Delivery |
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Effective date of registration: 20181206 Address after: 550001 two, phase 15 (B2) 15, Qi long central business building, Guiyang, Guizhou. Applicant after: Guizhou Taibo gynecological disease research center Address before: No. 10, yunyun Road, Yunyan District, Guiyang, Guizhou Applicant before: Li Lu |
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Effective date of registration: 20220826 Address after: No. 3, 15th Floor, Phase II (B2), Qilong Central Business Building, Guanshanhu District, Guiyang City, Guizhou Province, 550000 Patentee after: Guizhou Qianxiutang Skin Research Institute (L.P.) Address before: 550001 two, phase 15 (B2) 15, Qi long central business building, Guiyang, Guizhou. Patentee before: Guizhou Taibo gynecological disease research center |