CN101919789A

CN101919789A - Whitening anti-wrinkling rehabilitation liquid synthesized from epidermal growth factor and melatonin

Info

Publication number: CN101919789A
Application number: CN2010102190515A
Authority: CN
Inventors: 张玲; 魏强; 李强
Original assignee: SHANGHAI JIAOBEIYA FINE CHEMICAL CO Ltd
Current assignee: SHANGHAI JIAOBEIYA FINE CHEMICAL CO Ltd
Priority date: 2010-07-05
Filing date: 2010-07-05
Publication date: 2010-12-22

Abstract

The invention belongs to the technical fields of biological gene cosmetics and preparation thereof, and relates to a whitening anti-wrinkling rehabilitation liquid synthesized from an EGF (Epidermal Growth Factor) and melatonin. In order to keep the physical and chemical stability of the EGF and inhabit the decrease of the EGF activity, the invention promotes the cell differentiation of the EGF used in the skincare process through the stable modifying technology of the melatonin to the active components of the EGF and achieves more effective permeation, rehabilitation and enhancement of activity holding time at constant temperature; and the whitening anti-wrinkling rehabilitation liquid synthesized from the EGF and the melatonin contains a certain proportion of bioactive EGFs in the prior art and the melatonin, peptide, transparent lysergic acid, fucose, compound vitamins, mannite and fusion protein in the prior art, can be widely applied to a series of skincare products for skin rehabilitation, ageing resistance, whitening, freckle removal and the like and is one of ideal schemes for stably and persistently holding the activity of the EGF.

Description

The synthetic whitening anti-wrinkling rehabilitation liquid of epidermal growth factor and melatonin

Technical field

The invention belongs to the technical field of biological gene cosmetics and preparation thereof, relate to a kind of skin repair liquid that contains epidermal growth factor (EGF), particularly a kind of epidermal growth factor (EGF) and the synthetic whitening anti-wrinkling rehabilitation liquid of melatonin.

Background technology

HEGF's (Epidermal Growth Factor, hereafter " EGF ") is a kind of important somatomedin of human endocrine, is formed and comprised the short chain polypeptides of 3 two sulfur tendons by 53 amino acid residues; Have very strong biological effect, the EGF of denier can promote the division and the growth of Skin Cell strongly, has the metabolic effect of the epidermal growth of promotion, as treating burn, scald, promotion wound healing, repairing the damage of intestines and stomach, liver and a conjunctiva etc.

The effect of EGF comprises the function that strengthens cell inhibiting apoptogene BCL-2, resist and cause dna break in the cell, the foeign element of variation is (as ultraviolet, low temperature, medicine, hormone etc.) stimulation, prevent apoptosis, increase the capacity of resisting disturbance of skin, promote subcutaneous collagen protein, the extracellular hyaluronic acid, synthetic and secretion such as mucopolysaccharide, epidermis endocuticle moisture hold facility is strengthened, promote the propagation of Skin Cell, differentiation, quicken metabolism, has the extremely strong metabolic effect of promotion epidermal growth, improve new cell shared ratio in skin, reduce melanin and coloured corpuscle content in the skin simultaneously, facial mottle is desalinated gradually, disappear, then skin seems full, flexible, pore shrinks, wrinkle reduces, fundamentally stop the aging of skin, make that the skin delicacy is smooth, be in young state all the time.

Melatonin (Melatonin) from mammal cerebral part depths as size " pinus " excretory a kind of natural indoles hormone as the strobile, person " melatonin ".

Melatonin may produce free radical by removing when the body endoenzyme is urged reaction and non-enzymatic reaction, antioxidation and suppress lipid peroxidization protection cellularity, prevent DNA damage, reduce the content of body endoperoxide that there are tangible antagonism in the peroxidating that exogenous poisonous substance (as N,N'-dimethyl-.gamma..gamma.'-dipyridylium) is caused and the tissue injury that free radical caused of generation; Have the activating human body mononuclear cell, induce the excretory function of its cytotoxicity and IL-1, the damage that human body periphery lymphocyte chromosome is caused has significant protective effect, and the physics of free-radical generating and chemical mutagenicity and carcinogenecity are had antagonism; Can control and coordinate human immune system, nervous system, hormonal system, reproductive system and individual growth growth, have antitonic compressing, lipoid peroxidization resistant, physiologically active is widely arranged; Participate in activities such as calmness, hypnosis, analgesia and immunological effect.

The circadian rhythm of melatonin secretion has stronger adjusting time difference function, with advancing age, night self excretory melatonin levels reduction, directly have influence on the quality of sleep; From external additional melatonin, can make intravital melatonin levels maintain young state, adjust and the recovery circadian rhythm, have sleep assisting, improve the quality of living, the process of slow down aging, enhancing immunity, improve the functional status of whole health, angiocardiopathy preventing, cancer and various infirmities of age function, and, protection and repair are arranged to of the damage of its human body because of radiation-induced each system, each tissue and cellular elements.

Because EGF is a kind of biological activity protein, be a kind of active substance to the ambient temperature sensitivity, place one's entire reliance upon its biological activity of its magical effect generally adopts vacuum freeze drying technology to preserve, and needs before the use to dissolve with solvent; EGF is exposed in the air or after the dissolving, determines its active " disulfide bond " meeting oxidation scission, and molecular structure changes, its activity can only be preserved at most 7～10 days in air, otherwise EGF will lose activity, and deactivated EGF is the same with starch, does not have any pharmacological action; Therefore, if it is added in the cosmetics and skincare product, production and life cycle all will be above its active storage lives, make EGF ineffective, therefore, if can't the activity of EGF be kept, be exactly insignificant in the EGF adding skin care item then, because when consumer takes hands, the activity of EGF may have been lost, and can't play wrinkle removing, pore refining, promotion skin new life, function in delaying senility, so though some cosmetics has added EGF at present, but effect is also not obvious, even does not have effect at all, also has safety problem.

Real fine EGF skin care item, the biological activity that must use EGF keeps technology, otherwise EGF can only exist as a notion.

Summary of the invention

Purpose of the present invention: for physical and chemical stability and the active decline of inhibition EGF that keeps EGF, provide a kind of active component to add melatonin to EGF, by melatonin the EGF active component is played stable modification, and promote EGF to be used for the cell differentiation and the propagation of skin skin protection process, can be so that the EGF activity can access stable, and melatonin can stimulate the neural precursor propagation after the EGF effect, can partly substitute the function of EGF, reach more effective infiltration, and under the constant temperature active retention time strengthen, occupation mode more convenient with whitening anti-wrinkling rehabilitation liquid flexibly; Apply to solve the reparation of skin, defying age, the Comprehensive Treatment skin-care effect of serial skins such as the speckle of whitening.

The synthetic whitening anti-wrinkling rehabilitation liquid of epidermal growth factor of the present invention and melatonin comprises biological activity epidermal growth factor " EGF " and melatonin, and the EGF active component is separating or the production of use recombinant DNA technology from natural origin in the prior art; The content of EGF in repair liquid with total restatement 1 * 10 ^-2～1 * 10 ^-4In the % scope; In 0.01～1% scope, wherein nutritional solution contains silk peptide, the acid of transparent angle, trehalose, compound vitamin, mannitol, fusion rotein and acidity regulator to the content of melatonin in the prior art in repair liquid with total restatement; It is characterized in that: described biological activity EGF and melatonin are the repair liquid main body; Described its formed configuration (parts by weight):

EGF 1；

Melatonin 8～12;

Silk peptide 80～120;

Trehalose 400～600;

Compound vitamin 150～250;

Mannitol 150～250;

Transparent angle acid 800～1200;

Fusion rotein 5000～8000;

Disperse in the preparation process and dilute used: the citric acid solution of deionized water, normal saline or 0.1N.

Described its best allocation (parts by weight) is:

EGF 1；

Melatonin 10;

Silk peptide 100;

Trehalose 200;

Compound vitamin 200;

Mannitol 200;

Transparent angle acid 1000;

Fusion rotein 6000;

Described production technology may further comprise the steps:

(1) preparation of EGF-S solution:

With 1 part EGF lyophilized powder, 80～120 parts silk peptide, 400～600 parts keratohyalin is dissolved in normal saline (or citric acid solution of 0.1N), in 5～10 ℃ of water-baths, evenly stirs, and is diluted to 100000 parts EGF-S solution;

(2) preparation of EGF-M:

With 8～12 parts melatonin, be dissolved in 90000 parts of ionized waters, in 85～90 ℃ of water-baths, evenly stir 30min, behind the insulation 1h, be chilled to room temperature, stir, add acidity regulator, regulate pH value to 4～5, in batches, behind the EGF-S solution for preparing of adding by amount, add 5000～8000 parts fusion rotein, low speed evenly stirs 24h in the time of 0～20 ℃, regulate pH value, filter, after the lyophilization, make dry powder, vacuum packaging;

(3) preparation of nutritional solution:

With 400～～600 parts trehaloses, 150～250 parts compound vitamin, 150～250 parts mannitol and 400～800 parts transparent angle acid, be dissolved in 90000 parts 85～90 ℃ the ionized water, evenly stir 1h after, be chilled to room temperature, regulate pH value, make nutritional solution, standby; Described nutritional solution shields to the biological activity of EGF, participates in nutrition and nursing role to skin.

The preparation of described EGF-M or production technology:

With the lyophilized powder of 1 part of EGF, 8～12 parts melatonin, 80～120 parts silk peptide, 400～600 parts keratohyalin is dissolved in 100000 parts of deionized waters, in 85～90 ℃ of water-baths, after evenly stirring 1h, be chilled to room temperature, add 5000～8000 parts fusion rotein, low speed evenly stirs 24h, regulate pH value, filter, after the lyophilization, make dry powder, vacuum packaging.

It is characterized in that the pH value of described EGF-M and nutritional solution is 6～7, be fit to skin absorbs, the deactivation of having avoided acid, alkaloid substance to cause EGF.

It is characterized in that: described whitening anti-wrinkling rehabilitation liquid, nutritional solution is injected the EGF-M dry powder package, use the dissolving back fully.

Because of melatonin is stronger antioxidant, discover that it can antioxidation, suppress the nucleus DNA fracture that extraneous factor causes, melatonin easily accumulate in the nucleus than fat-solubility, after melatonin enters cell, can directly remove (.0H) and induce the GSH-PX increased activity, act on EGF, in cytoplasm and nuclear, carry out antioxidation, make EGF and constant temperature under the activity retention time longer; Synthetic by melatonin and EGF, the cell proliferation after the stimulation EGF effect improves the repair function of EGF to the cell of skin greatly, because the melatonin permeability is extremely strong, both synthetics make deep skin obtain repairing the easier skin that enters.

Specific implementation method

Therefore the inventor adds the proof that melatonin has carried out a lot of researchs and experiment mechanism to EGF, passing through a), culture medium adds melatonin (MT), b) culture medium adds epidermal growth factor (EGF), and c) culture medium adds EGF and three groups of experiment cultivations of MT observation, long-range effect one month; Observe and find the c group, be EGF and MT cultivation group, not only EGF is played Stabilization, the activity that has kept EGF can also stimulate the neural precursor propagation after the EGF effect, makes EGF cell differentiation faster, and by skin absorbs, not only have the effect of EGF but also have Metatonin whitening after adding melatonin, antiallergic, multiple beauty treatments such as removing free radical are more imitated.

For more clearly understanding feature of the present invention, below the invention will be further elaborated:

Embodiment 1

Biological activity epidermal growth factor (EGF) among the present invention, be the biological lyophilized powder EGF of existing related gene recombinant technique production and the melatonin in the prior art, silk peptide, the acid of transparent angle, trehalose, compound vitamin, mannitol, fusion rotein and acidity regulator; It is characterized in that: described biological activity EGF and melatonin are the repair liquid main body; Described its formed configuration (parts by weight):

EGF 1；

Melatonin 8～12;

Silk peptide 80～120;

Trehalose 400～600;

Compound vitamin 150～250;

Mannitol 150～250;

Transparent angle acid 800～1200;

Fusion rotein 5000～8000;

The synthetic whitening anti-wrinkling rehabilitation liquid of epidermal growth factor of the present invention and melatonin, described best allocation (parts by weight) is:

EGF 1；

Melatonin 10;

Silk peptide 100;

Trehalose 200;

Compound vitamin 200;

Mannitol 200;

Transparent angle acid 1000;

Fusion rotein 6000.

Embodiment 1

The synthetic whitening anti-wrinkling rehabilitation liquid of epidermal growth factor of the present invention and melatonin, described production technology may further comprise the steps:

(1) preparation of EGF-S solution:

With 1 part EGF lyophilized powder, 80～120 parts silk peptide, 400～600 parts keratohyalin is dissolved in citric acid (or acetum of 0.1N), in 5～10 ℃ of water-baths, evenly stirs, and is diluted to 100000 parts EGF-S solution;

(2) preparation of EGF-M:

With 8～12 parts melatonin, be dissolved in 90000 parts of ionized waters, in 85～90 ℃ of water-baths, evenly stir 30min, behind the insulation 1h, be chilled to room temperature, stir, regulate pH value to 4～5 with citric acid, in the time of 0～20 ℃ in batches, behind the EGF-S solution for preparing by the adding of amount, the fusion rotein that adds 5000～8000 parts, low speed evenly stirs 24h, regulates pH value 6～7, filter, after the lyophilization, make dry powder, vacuum packaging;

(3) preparation of nutritional solution:

With 400～600 parts trehaloses, 150～250 parts compound vitamin, 150～250 parts mannitol and 400～800 parts transparent angle acid, be dissolved in 90000 parts 85～90 ℃ the ionized water, after evenly stirring 1h, be chilled to room temperature, add citric acid, regulate pH value 6～7, make nutritional solution, standby; Described nutritional solution shields to the biological activity of EGF, participates in nutrition and nursing role to skin.

Embodiment 2

With the lyophilized powder of 1 part of EGF, 8～12 parts melatonin, 80～120 parts silk peptide, 400～600 parts keratohyalin is dissolved in 100000 parts of deionized waters, in 85～90 ℃ of water-baths, after evenly stirring 1h, be chilled to room temperature, add 5000～8000 parts fusion rotein, low speed evenly stirs 24h, adds citric acid, regulates pH value 6～7, filter, after the lyophilization, make dry powder, vacuum packaging.

The preparation of nutritional solution and production technology are identical with embodiment 1.

In order to remove some oxygen-derived free radicals that produce in the skin metabolism process, under the low situation of self activity level, regularly replenish EGF for Skin Cell, with regard to synthesizing of sustainable promotion cell, the EGF of denier just can promote the propagation and the growth of skin tissue cell, make newborn young cell replace old and feeble and dead cell rapidly, keep the epidermis metabolism.

Using method of the present invention:

Described whitening anti-wrinkling rehabilitation liquid injects nutritional solution the EGF-M dry powder package before use, and use the dissolving back fully.

Morning, earlier with warm water or cleansing milk cleaning skin, dry, with the dissolved whitening anti-wrinkling rehabilitation liquid of this nutritional solution, be coated in equably on the skin with brush, after 5～10 minutes, carry out other cosmetics or modification with the light finger forlement again; Can pure and fresh skin, sun-proof, remove oxygen-derived free radicals.

Just before going to bed, earlier with warm water or cleansing milk cleaning skin, dry, with the dissolved whitening anti-wrinkling rehabilitation liquid of this nutritional solution, be coated with equably with brush and touch on skin, after 10～20 minutes, skin is absorbed with the light finger forlement, the reuse wet towel is dried skin and is got final product; Promptly promote the regeneration of the metabolism and the cell of Skin Cell.

The synthetic whitening anti-wrinkling rehabilitation liquid of epidermal growth factor of the present invention and melatonin, by the stable modification technique of melatonin to the EGF active component, promote EGF to be used for the cell differentiation of skin protection process, reach more effective infiltration, the active retention time strengthens under reparation and the constant temperature, can be so that the EGF using method is more convenient and flexible; Can be widely used in the reparation to skin, series skin articles for use such as defying age, the speckle of whitening are that a kind of EGF activity can access one of ideal scheme of stable and lasting preservation at present.

The above, only be the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any those skilled in the art that are in the technical scope that the present invention discloses, variation that is equal to or replacement all should be encompassed within protection scope of the present invention; Therefore, protection scope of the present invention should be as the criterion with the protection domain of claim.

Claims

1. epidermal growth factor and the synthetic whitening anti-wrinkling rehabilitation liquid of melatonin, has the biological activity epidermal growth factor (hereinafter being called " EGF ") in certain proportioning prior art, melatonin in the prior art, silk peptide, the acid of transparent angle, trehalose, compound vitamin, mannitol, fusion rotein and acidity regulator; It is characterized in that: described biological activity EGF and melatonin are the repair liquid main body; Described its formed configuration (parts by weight):

EGF 1；

Melatonin 8～12;

Silk peptide 80～120;

Trehalose 400～600;

Compound vitamin 150～250;

Mannitol 150～250;

Transparent angle acid 800～1200;

Fusion rotein 5000～8000;

2. according to right 1 described epidermal growth factor and the synthetic whitening anti-wrinkling rehabilitation liquid of melatonin, it is characterized in that:

Described its best allocation (parts by weight) is:

EGF 1；

Melatonin 10;

Silk peptide 100;

Trehalose 200;

Compound vitamin 200;

Mannitol 200;

Transparent angle acid 1000;

Fusion rotein 6000.

3. according to the production technology of right 1,2 described epidermal growth factors and the synthetic whitening anti-wrinkling rehabilitation liquid of melatonin,

May further comprise the steps:

(1) preparation of EGF-S solution:

(2) preparation of EGF-M:

Melatonin with 8～12 parts, be dissolved in 90000 parts of ionized waters, evenly stir 1h, regulate pH value to 4～5 with acidity regulator, in the time of 0～20 ℃ in batches, behind 10000 parts of EGF-S solution preparing by the adding of amount, the fusion rotein that adds 5000～8000 parts, low speed evenly stirs 24h, regulates pH value, after the lyophilization, make dry powder, vacuum packaging;

(3) preparation of nutritional solution:

With 400～600 parts trehaloses, 150～250 parts compound vitamin, 150～250 parts mannitol and 400～800 parts transparent angle acid, be dissolved in 90000 parts 85～90 ℃ the ionized water, evenly stir 1h after, be chilled to room temperature, regulate pH value, make nutritional solution, standby.

4. according to the production technology of right 1,2 described epidermal growth factors and the synthetic whitening anti-wrinkling rehabilitation liquid of melatonin, it is characterized in that:

The preparation of EGF-M:

5. according to right 3,4 described epidermal growth factors and the synthetic whitening anti-wrinkling rehabilitation liquid of melatonin, it is characterized in that the pH value of described EGF-M and nutritional solution is 6～7.

6. according to right 1 described epidermal growth factor and the synthetic whitening anti-wrinkling rehabilitation liquid of melatonin, described acidity regulator is a citric acid.

7. according to right 1,2,3 described epidermal growth factors and the synthetic whitening anti-wrinkling rehabilitation liquid of melatonin, it is characterized in that: described whitening anti-wrinkling rehabilitation liquid, nutritional solution is injected the EGF-M dry powder package, use the dissolving back fully.