CN105916992A - 通过酶合成l-核酸 - Google Patents

通过酶合成l-核酸 Download PDF

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Publication number
CN105916992A
CN105916992A CN201480072215.2A CN201480072215A CN105916992A CN 105916992 A CN105916992 A CN 105916992A CN 201480072215 A CN201480072215 A CN 201480072215A CN 105916992 A CN105916992 A CN 105916992A
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CN
China
Prior art keywords
amino acid
acid sequence
seq
positions
polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201480072215.2A
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English (en)
Chinese (zh)
Inventor
A·佩什
F·雅罗什
M·雅恩兹
S·克鲁斯曼
R·大卫
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arterian Biotechnology Inc
Original Assignee
Noxxon Pharma AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Noxxon Pharma AG filed Critical Noxxon Pharma AG
Publication of CN105916992A publication Critical patent/CN105916992A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01007—Mannokinase (2.7.1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201480072215.2A 2013-11-20 2014-11-20 通过酶合成l-核酸 Pending CN105916992A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP13005449.7 2013-11-20
EP13005449 2013-11-20
PCT/EP2014/003102 WO2015074756A1 (en) 2013-11-20 2014-11-20 Synthesis of l-nucleic acids by means of an enzyme

Publications (1)

Publication Number Publication Date
CN105916992A true CN105916992A (zh) 2016-08-31

Family

ID=49622663

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201480072215.2A Pending CN105916992A (zh) 2013-11-20 2014-11-20 通过酶合成l-核酸

Country Status (6)

Country Link
US (1) US11634741B2 (enExample)
EP (1) EP3071702B1 (enExample)
JP (1) JP6670237B2 (enExample)
KR (1) KR20160079126A (enExample)
CN (1) CN105916992A (enExample)
WO (1) WO2015074756A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106318924A (zh) * 2016-10-26 2017-01-11 江南大学 一种催化dna合成延伸能力提高的dna聚合酶
CN112105748A (zh) * 2018-04-05 2020-12-18 清华大学 测序和生产核酸序列的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106467910A (zh) * 2015-08-18 2017-03-01 清华大学 L-dna/l-rna聚合酶及其应用
ES2846949T3 (es) 2015-11-16 2021-07-30 Stratos Genomics Inc Variantes de la polimerasa DPO4
WO2018204707A1 (en) * 2017-05-04 2018-11-08 Stratos Genomics Inc. Dp04 polymerase variants
AU2018383610B2 (en) * 2017-12-11 2024-09-12 F. Hoffmann-La Roche Ag DPO4 polymerase variants with improved accuracy
KR102285583B1 (ko) * 2020-01-09 2021-08-05 한국과학기술연구원 L-dna와 결합하는 자연계 단백질 및 이의 용도
MX2023001604A (es) * 2020-08-06 2023-09-05 Univ Tsinghua Síntesis química de proteínas grandes y especulares y usos de las mismas.
WO2025082960A1 (en) * 2023-10-18 2025-04-24 F. Hoffmann-La Roche Ag Dpo4 polymerase variants with improved properties

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008856A2 (de) * 1996-08-30 1998-03-05 Fuerste Jens Peter Spiegelselektion und spiegelevolution von nucleinsäuren
DE10156274A1 (de) * 2001-11-16 2003-06-12 Noxxon Pharma Ag Verfahren zur Synthese von all-D-Polypeptiden
WO2003047743A2 (de) * 2001-11-16 2003-06-12 Noxxon Pharma Ag Verfahren zur synthese von all-d-peptide und peptidnukleinsäuren
WO2005113760A2 (en) * 2004-05-20 2005-12-01 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Thermostable y-family polymerases and chimeras

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2873452A1 (en) * 2012-05-16 2013-11-21 Noxxon Pharma Ag Enzymatic synthesis of l-nucleic acids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008856A2 (de) * 1996-08-30 1998-03-05 Fuerste Jens Peter Spiegelselektion und spiegelevolution von nucleinsäuren
DE10156274A1 (de) * 2001-11-16 2003-06-12 Noxxon Pharma Ag Verfahren zur Synthese von all-D-Polypeptiden
WO2003047743A2 (de) * 2001-11-16 2003-06-12 Noxxon Pharma Ag Verfahren zur synthese von all-d-peptide und peptidnukleinsäuren
WO2005113760A2 (en) * 2004-05-20 2005-12-01 The Government Of The United States Of America, Represented By The Secretary Of The Department Of Health And Human Services Thermostable y-family polymerases and chimeras

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106318924A (zh) * 2016-10-26 2017-01-11 江南大学 一种催化dna合成延伸能力提高的dna聚合酶
WO2018076980A1 (zh) * 2016-10-26 2018-05-03 江南大学 一种催化dna合成延伸能力提高的dna聚合酶
CN112105748A (zh) * 2018-04-05 2020-12-18 清华大学 测序和生产核酸序列的方法
CN112105748B (zh) * 2018-04-05 2023-08-04 清华大学 测序和生产核酸序列的方法

Also Published As

Publication number Publication date
WO2015074756A1 (en) 2015-05-28
KR20160079126A (ko) 2016-07-05
US20160304926A1 (en) 2016-10-20
EP3071702A1 (en) 2016-09-28
EP3071702C0 (en) 2025-07-16
JP6670237B2 (ja) 2020-03-25
EP3071702B1 (en) 2025-07-16
US11634741B2 (en) 2023-04-25
JP2016537981A (ja) 2016-12-08

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SE01 Entry into force of request for substantive examination
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TA01 Transfer of patent application right

Effective date of registration: 20191106

Address after: Berlin

Applicant after: Arterian biotechnology, Inc

Address before: Berlin

Applicant before: Nozon Pharmaceutical Co., Ltd.

WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20160831