CN105891283A - Medicine effect quantification device and method for elemene - Google Patents

Abstract

The invention discloses a medicine effect quantification device and method for elemene. The peak of the signal to noise ratio of elemene with an unknown medicine effect is compared with the peak of the signal to noise ratio of a common cancer therapy medicine cis-platinum, and the medicine effect and medicine quantity of the elemene are obtained by referring to cis-platinum with the clear medicine effect and medicine quantity. The medicine effect quantification device has the advantages of being high in detection efficiency and good in accuracy, providing reliable basis for safety usage of the elemene and ensuring the safety of a patient.

Description

Knock-down gasifying device and method for elemene

Technical field

The present invention relates to medicine effect detection technique field, especially relate to a kind of can accurately detect drug effect for olive The knock-down gasifying device of fragrant alkene and method.

Background technology

The dosage of cancer therapy drug accurately when dose deficiency, must can not reach therapeutic effect.When dose is beyond safety Dosage, then can cause the gastrointestinal reaction such as loss of appetite, nausea,vomiting,diarrhea.The most serious common toxic reaction is kidney poison Property reaction, repeated drug taking can aggravate nephrotoxicity reaction, major determinant kidney proximal tubule, make Vacuole formation, exuviation, tube chamber , there is hyaline cast in expansion, and in blood, uric acid is too much, can cause the symptoms such as hematuria time serious.

When heavy dose and repeatedly medication, it may appear that neurotoxicity, ear Ke hair cell for mouthpart can be damaged, cause height Frequency loses to be listened.

The drug effect of PTS does not has clinical data to be referred to, and when the initial stage uses, fully rely on doctor gropes judgement, If during dose deficiency, not reaching therapeutic effect；If dose can cause serious toxic reaction beyond safe dose, can give and suffer from Person's health brings very major injury.

Summary of the invention

The goal of the invention of the present invention is to overcome the deficiency that the drug effect of PTS of the prior art is not clear, it is provided that A kind of knock-down gasifying device for elemene that can accurately detect drug effect and method.

To achieve these goals, the present invention is by the following technical solutions:

A kind of knock-down gasifying device for elemene, including operating platform, the electrochemical operation being located on operating platform Stand, flow cell and adjustable for height support arm, the 2 pieces of substrates being located in flow cell, be located at the electrode chip on every piece of substrate, if Two montants on flow cell；Support arm is provided with and fills the first solution transfusion bottle of cleaning solution, fills elemene solution Second solution transfusion bottle, the first solution transfusion bottle bottom is provided with the first micro pump of UNICOM, and the second solution transfusion bottle bottom is provided with UNICOM Second micro pump, the liquid outlet of the first micro pump and the second micro pump is with the spiral catheter being located on two montants even Connect；Each electrode chip all include being connected with electrochemical workstation to electrode, reference electrode and working electrode, each work electricity Extremely going up all to cultivate and have several cancerous cell, flow cell is provided with drain lid, and drain lid is equipped with several delivery holes, flow cell Bottom is provided with drain pipe；Described catheter outer peripheral face is provided with heating film；Electrochemical workstation, the first micro pump, the second trace Pump and heating film all electrically connect with computer.

When elemene solution drips on cancerous cell, cancerous cell can react, the electric current that electrochemical workstation detects Density signal can occur respective change, and detection signal Spect (t) of detected new drug is inputted and is pre-stored in computer by the present invention In second order stochastic resonance system in, computer draws the signal to noise ratio spectrogram of excitation noise signal, chooses in signal to noise ratio spectrogram Near the signal-to-noise ratio peak of the characteristic peak of initial point, and by the coordinate (M of signal-to-noise ratio peak₁, M₂) store in computer, computer Middle storage has the characteristic peaks coordinate (W of cisplatin₁, W₂)；

WhenAnd | M₂| ＞ | W₂|, Computer display elemene is better than cisplatin to the action effect of cancerous cell, olive The dosage of fragrant alkene needs to reduce than cisplatin；

Work as M₁＞ W₁AndTime, Computer display elemene is poorer than cisplatin to the action effect of cancerous cell, olive The dosage of fragrant alkene needs to increase than cisplatin.

The present invention is by the signal to noise ratio of the signal-to-noise ratio peak of the elemene of unknown drug effect with conventional cancer treatment drugs cisplatin Peak value compares, and under the reference of the cisplatin of the clearest and the most definite drug effect and dose, it is thus achieved that the drug effect of elemene and dose, pacifies for elemene Full use provides reliable basis, it is ensured that the safety of patient.

As preferably, described support arm includes the armstand being connected with operating platform and is located at the horizontal arm of armstand upper end, the It is equipped with hanging piece on one solution transfusion bottle and the second solution transfusion bottle；Each hanging piece all includes annular canister, is located at the end of annular canister lower end Plate and two the vertical connecting rods being located at annular canister upper end；It is equipped with on each base plate and hangs with the first solution transfusion bottle or the second solution The corresponding perforate of bottle neck of bottle；Horizontal arm is connected with each vertical connecting rod upper end.

As preferably, described armstand includes inner sleeve and the trocar sheath being connected with inner sleeve, and inner sleeve is provided with several Elastic bumps, trocar sheath is provided with several through holes corresponding with elastic bumps.

As preferably, flow cell includes the housing of upper end open, the cover plate being located on housing and the sealing being located on cover plate Circle, sealing ring lower surface is connected with to the base plate seals outside electrode, working electrode and be provided with logical to the substrate between electrode Hole.

A kind of method of knock-down gasifying device for elemene, comprises the steps:

(5-1) support arm being adjusted to suitable height, computer controls heating film electrified regulation, and the first micro pump drips Cleaning solution, cleaning solution flows through during catheter heated, and the first micro pump dropping liquid is after 15 to 25 minutes, and computer controls first Micro pump stops dropping liquid；

(5-2) computer controls the second micro pump and drips elemene solution, and the second micro pump dropping liquid is after 12 to 15 minutes, meter Calculation machine gathers 2 current density signal S of electrochemical workstation detection₁(t), S₂T (), computer calculates and obtains S₁(t), S₂ T the average signal of (), is defined as current density signal S (t) by average signal；

Computer controls current density signal S (t) of the second micro pump computer acquisition electrochemical workstation detection, calculates The sampled value that several time intervals are Δ t chosen in current density signal S (t) by machine, and each sampled value is according to time order and function Order is arranged to make up detecting signal ES (t)；

(5-3) for each sampled value ES (t outside in ES (t) first sampled value and last sampled value₁), profit Use formulaCalculate steady coefficient ratio；

Computer is previously provided with the weight threshold 0.55,1 and 1.45 increased successively；

Sampled value in the range of [1-A1,1+A1] is positioned at for ratio, sampled value is modified to B1ES (t₁), B1 is little In the real number of 0.4；

Sampled value in the range of (0.55,1-A1) or (1+A1,1.45) is positioned at for ratio, sampled value is modified to B2ES(t₁),

(5-4) replace the corresponding sampled value in ES (t) by each sampled value corrected, obtain the detection letter through revising Number Spect (t),

In the second order stochastic resonance system be pre-stored in Spect (t) input in computer, computer draws excitation noise letter Number signal to noise ratio spectrogram, signal to noise ratio spectrogram is chosen the signal-to-noise ratio peak of characteristic peak near initial point, and by signal-to-noise ratio peak Coordinate (M₁, M₂) store in computer, in computer, storage has the characteristic peaks coordinate (W of cisplatin₁, W₂)；

(5-5) whenAnd | M₂| ＞ | W₂|, the action effect of cancerous cell is compared cisplatin by Computer display elemene Good, the dosage of elemene needs to reduce than cisplatin；

Work as M₁＞ W₁AndTime, Computer display elemene is poorer than cisplatin to the action effect of cancerous cell, olive The dosage of fragrant alkene needs to increase than cisplatin.

As preferably, in the described second order stochastic resonance system that Spect (t) input is pre-stored in computer, computer The signal to noise ratio spectrogram drawing excitation noise signal comprises the steps:

Spect (t) is inputted single order accidental resonance model by computer

In, Wherein, (x, t) is potential function to V, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are the constant set, and ξ (t) is Outer noise, D is outer noise intensity, and N (t) grasps noise in being,For periodic sinusoidal signal, A is signal amplitude, F is signal frequency, and t is movement time,For phase place, ifcx²-dx⁴For mark Determine component；

Computer calculate V (x, t) for first derivative and the second dervative of x, and makes equation be equal to 0, obtain two layers with Machine resonance model:

Set noise intensity D=0,Spect (t)=0, N (t)=0；It is calculated the marginal value of A For

The marginal value of A is substituted in one layer of accidental resonance model, and sets X₀(t)=0, sn₀=0, with quadravalence jade for asking rain Ge Kuta One layer of accidental resonance model of Algorithm for Solving, obtains And calculate ${\left(k_1\right)}_n=4({\mathrm{ax}}_{n-1}\left(t\right)-{\mathrm{bx}}_{n-1}^3\left(t\right)+{\mathrm{sn}}_{n-1}),$

${\left(k_2\right)}_n=4\[a\left(x_{n-1}\right(t)+\frac{{\left(k_1\right)}_{n-1}}{2})-b{\left(x_{n-1}\right(t)+\frac{{\left(k_1\right)}_{n-1}}{2})}^3+{\mathrm{sn}}_{n-1}\],$

${\left(k_3\right)}_n=4\[a\left(x_{n-1}\right(t)+\frac{{\left(k_2\right)}_{n-1}}{2})-b{\left(x_{n-1}\right(t)+\frac{\sqrt{2}-1}{2}{\left(k_1\right)}_{n-1}+\frac{2-\sqrt{2}}{2}{\left(k_2\right)}_{n-1})}^3+{\mathrm{sn}}_{n+1}\],$

${\left(k_4\right)}_n=4\[a\left(x_{n-1}\right(t)+{\left(k_3\right)}_{n-1})-b{\left(x_{n-1}\right(t)-\frac{\sqrt{2}}{2}{\left(k_2\right)}_{n-1}+\frac{2+\sqrt{2}}{2}{\left(k_3\right)}_{n-1})}^3+{\mathrm{sn}}_{n+1}\];$

Wherein, x_nT () is the n order derivative of x (t), sn_n-1It is the n-1 order derivative of S (t) value at t=0, sn_n+1It is S The n+1 order derivative of (t) value at t=0, n=0,1 ..., N-1；Obtain x₁(t), x₂(t) ..., x_n+1The value of (t)；

Computer is to x₁(t), x₂(t) ..., x_n+1T () is integrated, obtain x (t), and obtain x (t) at one layer the most altogether The second order stochastic resonance system of model and two layers of accidental resonance model composition of shaking produces the position x in accidental resonance moment_mValue and x_m Corresponding resonance moment t₁And and t₁Corresponding noise D₁, D₁For a value in D；

Computer utilizes formulaCalculate the letter of second order stochastic resonance system output Make an uproar ratio；Wherein, Δ U=a²/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal.

As preferably, also including the agitator (15) being located in the second solution transfusion bottle bottom, agitator is electrically connected with computer Connect；Also include between step (5-1) and (5-2) that computer controls to stop stirring after agitator stirs 12 to 14 minutes.

As preferably, also including the temperature sensor (16) being located on catheter, temperature sensor electrically connects with computer；

Also comprise the steps:

The temperature of temperature sensor detection catheter, is provided with the interval [T of standard temperature in computer_L, T_H], when the temperature of detection Degree >=T_H, computer controls heating film and stops heating；Temperature≤T when detection_L, computer controls heating film and begins to warm up.

As preferably, also comprise the steps: after step (5-5)

WhenTime, the dose that Computer display suggestion is increased or decreased is 3% to 20%；

WhenTime, the dose that Computer display suggestion is increased or decreased is 20% to 35%；

WhenTime, the dose that Computer display suggestion is increased or decreased is more than 35%.

As preferably, the span of A1 is 0.14 to 0.32；The span of B1 is 0.2 to 0.28, the value model of B2 Enclose is 0.32 to 0.48.

Therefore, there is advantages that detection efficiency is high, accuracy is good, provide for elemene safe handling Reliable basis, it is ensured that the safety of patient.

Accompanying drawing explanation

Fig. 1 is a kind of structural representation of the present invention.

Fig. 2 is the theory diagram of the present invention；

Fig. 3 is a kind of structural representation of the electrode chip of the present invention；

Fig. 4 is a kind of flow chart of embodiments of the invention；

Fig. 5 is a kind of signal to noise ratio spectrogram of the cisplatin of the present invention；

Fig. 6 is a kind of structural representation of the drain lid of the present invention.

In figure: operating platform 1, electrochemical workstation 2, flow cell 3, support arm 4, substrate 5, electrode chip 6, montant 7, One solution transfusion bottle the 8, second solution transfusion bottle the 9, first micro pump the 10, second micro pump 11, catheter 12, computer 13, hanging piece 14, Agitator 15, temperature sensor 16, drain lid 17, delivery hole 18, drain pipe 31, armstand 41, horizontal arm 42, through hole 51, paint Coating 52, to electrode 61, reference electrode 62, working electrode 63, heating film 121, annular canister 141, vertical connecting rod 142, inner sleeve Pipe 411, trocar sheath 412.

Detailed description of the invention

The present invention will be further described with detailed description of the invention below in conjunction with the accompanying drawings.

Embodiment as shown in Figure 1, Figure 2, Figure 3 shows is a kind of knock-down gasifying device for elemene, including operating platform 1, electrochemical workstation 2, flow cell 3 and the adjustable for height support arm 4 being located on operating platform, 2 pieces be located in flow cell Substrate 5, the electrode chip 6 being located on every piece of substrate, two montants 7 being located on flow cell；Support arm is provided with and fills cleaning First solution transfusion bottle 8 of solution, filling the second solution transfusion bottle 9 of elemene solution, the first solution transfusion bottle bottom is provided with UNICOM First micro pump 10, the second solution transfusion bottle bottom is provided with the second micro pump 11 of UNICOM, the first micro pump and the second micro pump Liquid outlet is connected with the spiral catheter 12 being located on two montants；Each electrode chip all includes and electrochemical operation Stand connect to electrode 61, reference electrode 62 and working electrode 63, on each working electrode all cultivate have 30000 cancerous cell, Flow cell bottom is provided with drain pipe 31；Catheter outer peripheral face is provided with heating film 121.

As shown in Figure 6, the flow cell corresponding with catheter lower end is provided with drain lid 17, with working electrode and to electrode Between corresponding drain lid be provided with 8 delivery holes 18, drain lid edge be provided with annular vertical edge.

As in figure 2 it is shown, electrochemical workstation, the first micro pump, the second micro pump and heating film are all electrically connected with computer 13 Connect.Each substrate all uses gold copper-base alloy to make.

As it is shown on figure 3, each electrode chip all include being connected with electrochemical workstation to electrode 61, reference electrode 62 and Working electrode 63, also includes painting layer 52.

As it is shown in figure 1, support arm includes the armstand 41 being connected with operating platform and the horizontal arm 42 being located at armstand upper end, the It is equipped with hanging piece 14 on one solution transfusion bottle and the second solution transfusion bottle；Each hanging piece all includes annular canister 141, is located at annular canister lower end Base plate and be located at two vertical connecting rods 142 of annular canister upper end；It is equipped with on each base plate and the first solution transfusion bottle or The perforate that the bottle neck of two solution transfusion bottles is corresponding；Horizontal arm is connected with each vertical connecting rod upper end.

Armstand includes inner sleeve 411 and the trocar sheath 412 being connected with inner sleeve, and it is convex that inner sleeve is provided with several elasticity Rising, trocar sheath is provided with several through holes corresponding with elastic bumps.

Flow cell includes the housing of upper end open, the cover plate being located on housing and the sealing ring being located on cover plate, sealing ring Lower surface is connected with to the base plate seals outside electrode, working electrode and the substrate between electrode is provided with through hole 51.

As shown in Figure 4, a kind of method of knock-down gasifying device for elemene, comprise the steps:

Computer controls to stop stirring after two agitators stir 14 minutes；

Step 100, preheats, cleans

Support arm is adjusted to suitable height, the temperature of temperature sensor detection catheter, computer is provided with standard Temperature range [T_L, T_H], as the temperature >=T of detection_H, computer controls heating film and stops heating；Temperature≤T when detection_L, meter Calculation machine controls heating film and begins to warm up；T_L=36.5 DEG C, T_H=39.6 DEG C；

First micro pump drips cleaning solution, and cleaning solution flows through during catheter heated, the first micro pump dropping liquid 25 points Zhong Hou, computer controls the first micro pump and stops dropping liquid；Cleaning solution is phosphate buffer；Computer controls agitator stirring Stirring is stopped after 10 minutes；

Step 200, detection

Computer controls the second micro pump and drips elemene solution, after the second micro pump dropping liquid 15 minutes, computer acquisition 2 current density signal S of electrochemical workstation detection₁(t), S₂T (), computer calculates and obtains S₁(t), S₂(t) average Signal, is defined as current density signal S (t) by average signal；The electric current density letter of computer acquisition electrochemical workstation detection Number S (t), computer chooses the sampled value that several time intervals are Δ t in current density signal S (t), and each sampled value is pressed It is arranged to make up detecting signal ES (t) according to time order and function order；

Step 300, data correction processes

For each sampled value ES (t outside in ES (t) first sampled value and last sampled value₁), utilize public affairs FormulaCalculate steady coefficient ratio；

Computer is previously provided with the weight threshold 0.55,1 and 1.45 increased successively；

Sampled value in the range of [1-A1,1+A1] is positioned at for ratio, sampled value is modified to B1ES (t₁), A1 is 0.25, B1 is 0.28；

Sampled value in the range of (0.55,1-A1) or (1+A1,1.45) is positioned at for ratio, sampled value is modified to B2ES(t₁), B2 is 0.48；

Step 400, the signal-to-noise ratio peak at selected characteristic peak

Replace the corresponding sampled value in ES (t) by each sampled value corrected, obtain the detection signal through revising Spect (t),

Spect (t) is inputted single order accidental resonance model by computer

In, Wherein, (x, t) is potential function to V, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are the constant set, and ξ (t) is Outer noise, D is outer noise intensity, and N (t) grasps noise in being,For periodic sinusoidal signal, A is signal amplitude, F is signal frequency, and t is movement time,For phase place, ifcx²-dx⁴For mark Determine component；

Computer calculate V (x, t) for first derivative and the second dervative of x, and makes equation be equal to 0, obtain two layers with Machine resonance model:

Set noise intensity D=0,Spect (t)=0, N (t)=0；It is calculated the marginal value of A For

The marginal value of A is substituted in one layer of accidental resonance model, and sets X₀(t)=0, sn₀=0, with quadravalence jade for asking rain Ge Kuta One layer of accidental resonance model of Algorithm for Solving, obtains And calculate ${\left(k_1\right)}_n=4({\mathrm{ax}}_{n-1}\left(t\right)-{\mathrm{bx}}_{n-1}^3\left(t\right)+{\mathrm{sn}}_{n-1}),$

${\left(k_2\right)}_n=4\[a\left(x_{n-1}\right(t)+\frac{{\left(k_1\right)}_{n-1}}{2})-b{\left(x_{n-1}\right(t)+\frac{{\left(k_1\right)}_{n-1}}{2})}^3+{\mathrm{sn}}_{n-1}\],$

${\left(k_3\right)}_n=4\[a\left(x_{n-1}\right(t)+\frac{{\left(k_2\right)}_{n-1}}{2})-b{\left(x_{n-1}\right(t)+\frac{\sqrt{2}-1}{2}{\left(k_1\right)}_{n-1}+\frac{2-\sqrt{2}}{2}{\left(k_2\right)}_{n-1})}^3+{\mathrm{sn}}_{n+1}\],$

${\left(k_4\right)}_n=4\[a\left(x_{n-1}\right(t)+{\left(k_3\right)}_{n-1})-b{\left(x_{n-1}\right(t)-\frac{\sqrt{2}}{2}{\left(k_2\right)}_{n-1}+\frac{2+\sqrt{2}}{2}{\left(k_3\right)}_{n-1})}^3+{\mathrm{sn}}_{n+1}\];$

Wherein, x_nT () is the n order derivative of x (t), sn_n-1It is the n-1 order derivative of S (t) value at t=0, sn_n+1It is S The n+1 order derivative of (t) value at t=0, n=0,1 ..., N-1；Obtain x₁(t), x₂(t) ..., x_n+1The value of (t)；

Computer is to x₁(t), x₂(t) ..., x_n+1T () is integrated, obtain x (t), and obtain x (t) at one layer the most altogether The second order stochastic resonance system of model and two layers of accidental resonance model composition of shaking produces the position x in accidental resonance moment_mValue and x_m Corresponding resonance moment t₁And and t₁Corresponding noise D₁, D₁For a value in D；

Computer utilizes formulaCalculate the letter of second order stochastic resonance system output Make an uproar ratio；Wherein, Δ U=a²/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal.

The signal-to-noise ratio peak of the characteristic peak near initial point is chosen in signal to noise ratio spectrogram, and by the coordinate of signal-to-noise ratio peak (M₁, M₂) store in computer, in computer, storage has the characteristic peaks coordinate (W of cisplatin₁, W₂)；

Step 500, makes drug effect and dose judges

WhenAnd | M₂| ＞ | W₂|, Computer display elemene is better than cisplatin to the action effect of cancerous cell, olive The dosage of fragrant alkene needs to reduce than cisplatin；

Work as M₁＞ W₁AndTime, Computer display elemene is poorer than cisplatin to the action effect of cancerous cell, olive The dosage of fragrant alkene needs to increase than cisplatin；

WhenTime, the dose that Computer display suggestion is increased or decreased is 3% to 20%；

WhenTime, the dose that Computer display suggestion is increased or decreased is 20% to 35%；

WhenTime, the dose that Computer display suggestion is increased or decreased is more than 35%.

Characteristic peaks coordinate (the W of cisplatin₁, W₂) use the step 100 of the present invention to obtain, respectively to 400 detection cisplatin solution The cisplatin solution being contained in 2 the second solution transfusion bottles is identical with elemene concentration.

As it is shown in figure 5, the signal-to-noise ratio peak of the characteristic peak of cisplatin is (20 ,-34dB), the characteristic peak of the elemene of the present invention Signal-to-noise ratio peak be (10 ,-38dB)；

Then Computer display elemene is better than cisplatin to the action effect of cancerous cell, and the dosage of elemene needs to compare cisplatin The information reduced；AndTherefore, it can suggestion dose and reduce 30%.

Should be understood that the present embodiment is merely to illustrate the present invention rather than limits the scope of the present invention.In addition, it is to be understood that After having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, these etc. Valency form falls within the application appended claims limited range equally.

Claims (10)

1., for a knock-down gasifying device for elemene, it is characterized in that, including operating platform (1), be located on operating platform Electrochemical workstation (2), flow cell (3) and adjustable for height support arm (4), the 2 pieces of substrates (5) being located in flow cell, it is located at Electrode chip (6) on every piece of substrate, two montants (7) being located on flow cell；Support arm is provided with and fills cleaning solution First solution transfusion bottle (8), filling the second solution transfusion bottle (9) of elemene solution, the first solution transfusion bottle bottom is provided with the of UNICOM One micro pump (10), the second solution transfusion bottle bottom is provided with second micro pump (11) of UNICOM, the first micro pump and the second micro pump Liquid outlet be connected with the spiral catheter (12) being located on two montants；Each electrode chip all includes and electrochemistry Work station connect to electrode (61), reference electrode (62) and working electrode (63), on each working electrode all cultivation have some Individual cancerous cell, flow cell is provided with drain lid (17), and drain lid is equipped with several delivery holes (18), and flow cell bottom is provided with Drain pipe (31)；Described catheter outer peripheral face is provided with heating film (121)；Electrochemical workstation, the first micro pump, the second trace Pump and heating film all electrically connect with computer (13).

Knock-down gasifying device for elemene the most according to claim 1, is characterized in that, described support arm includes and behaviour Make the armstand (41) that platform connects and the horizontal arm (42) being located at armstand upper end, on the first solution transfusion bottle and the second solution transfusion bottle all It is provided with hanging piece (14)；Each hanging piece all includes annular canister (141), is located at the base plate of annular canister lower end and is located at annular canister upper end Article two, vertical connecting rod (142)；It is equipped with corresponding with the bottle neck of the first solution transfusion bottle or the second solution transfusion bottle on each base plate Perforate；Horizontal arm is connected with each vertical connecting rod upper end.

Knock-down gasifying device for elemene the most according to claim 2, is characterized in that, described armstand includes inner sleeve (411) trocar sheath (412) and with inner sleeve being connected, inner sleeve is provided with several elastic bumps, and trocar sheath is provided with and bullet Property protruding several corresponding through holes.

4. according to the knock-down gasifying device for elemene described in claim 1 or 2 or 3, it is characterized in that, flow cell includes The housing of end opening, the cover plate being located on housing and the sealing ring being located on cover plate, sealing ring lower surface with to electrode outside Base plate seals connects, working electrode and the substrate between electrode is provided with through hole (51).

5. a method based on the knock-down gasifying device for elemene described in claim 1, is characterized in that, including as follows Step:

(5-1) support arm being adjusted to suitable height, computer controls heating film electrified regulation, and the first micro pump drips cleaning Solution, cleaning solution flows through during catheter heated, and the first micro pump dropping liquid is after 15 to 25 minutes, and computer controls the first trace Pump stops dropping liquid；

(5-2) computer controls the second micro pump and drips elemene solution, and the second micro pump dropping liquid is after 12 to 15 minutes, computer Gather 2 current density signal S of electrochemical workstation detection₁(t), S₂T (), computer calculates and obtains S₁(t), S₂(t) Average signal, is defined as current density signal S (t) by average signal；

Computer controls current density signal S (t) of the second micro pump computer acquisition electrochemical workstation detection, and computer exists Choosing the sampled value that several time intervals are Δ t in current density signal S (t), each sampled value is according to time order and function order It is arranged to make up detecting signal ES (t)；

(5-3) for each sampled value ES (t outside in ES (t) first sampled value and last sampled value₁), utilize public affairs FormulaCalculate steady coefficient ratio；

Computer is previously provided with the weight threshold 0.55,1 and 1.45 increased successively；

Sampled value in the range of [1-A1,1+A1] is positioned at for ratio, sampled value is modified to B1 ES (t₁), B1 is less than 0.4 Real number；

Sampled value in the range of (0.55,1-A1) or (1+A1,1.45) is positioned at for ratio, sampled value is modified to B2 ES (t₁),

(5-4) replace the corresponding sampled value in ES (t) by each sampled value corrected, obtain the detection signal through revising Spect (t),

In the second order stochastic resonance system be pre-stored in Spect (t) input in computer, computer draws excitation noise signal Signal to noise ratio spectrogram, chooses the signal-to-noise ratio peak of the characteristic peak near initial point in signal to noise ratio spectrogram, and by the seat of signal-to-noise ratio peak Mark (M₁, M₂) store in computer, in computer, storage has the characteristic peaks coordinate (W of cisplatin₁, W₂)；

(5-5) whenAnd | M₂| ＞ | W₂|, Computer display elemene is better than cisplatin to the action effect of cancerous cell, olive The dosage of fragrant alkene needs to reduce than cisplatin；

Work as M₁＞ W₁ AndTime, Computer display elemene is poorer than cisplatin to the action effect of cancerous cell, The dosage of elemene needs to increase than cisplatin.

The method of the knock-down gasifying device for elemene the most according to claim 5, is characterized in that,

In described second order stochastic resonance system Spect (t) input being pre-stored in computer, computer draws excitation noise letter Number signal to noise ratio spectrogram comprise the steps:

Spect (t) is inputted single order accidental resonance model by computer

In, wherein, (x, t) is potential function to V, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are the normal of setting Number, ξ (t) is outer noise, and D is outer noise intensity, and N (t) grasps noise in being,For periodic sinusoidal signal, A is Signal amplitude, f is signal frequency, and t is movement time,For phase place, if cx²-dx⁴For demarcating component；

Computer calculates V, and (x, t) for first derivative and the second dervative of x, and makes equation be equal to 0, obtains two layers the most altogether Shake model:

Set noise intensity D=0,Spect (t)=0, N (t)=0；The marginal value being calculated A is

The marginal value of A is substituted in one layer of accidental resonance model, and sets X₀(t)=0, sn₀=0, with quadravalence jade for asking rain Ge Kuta algorithm Solve one layer of accidental resonance model, obtain And calculate

Wherein, x_nT () is the n order derivative of x (t), sn_n-1It is the n-1 order derivative of S (t) value at t=0, sn_n+1It is the n+ of S (t) 1 order derivative value at t=0, n=0,1 ..., N-1；Obtain x₁(t), x₂(t) ..., x_n+1The value of (t)；

Computer is to x₁(t), x₂(t) ..., x_n+1T () is integrated, obtain x (t), and obtain x (t) at one layer of accidental resonance mould The second order stochastic resonance system of type and two layers of accidental resonance model composition produces the position x in accidental resonance moment_mValue and x_mRelatively The resonance moment t answered₁And and t₁Corresponding noise D₁, D₁For a value in D；

Computer utilizes formula

Calculate the output of second order stochastic resonance system Signal to noise ratio；Wherein, Δ U=a²/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal.

The method of the knock-down gasifying device for elemene the most according to claim 5, is characterized in that, also includes being located at Agitator (15) in two solution transfusion bottle bottoms, agitator electrically connects with computer；Also include between step (5-1) and (5-2) Computer controls to stop stirring after agitator stirs 12 to 14 minutes.

The method of the knock-down gasifying device for elemene the most according to claim 5, is characterized in that, also includes being located at and leads Temperature sensor (16) on liquid pipe, temperature sensor electrically connects with computer；Also comprise the steps:

The temperature of temperature sensor detection catheter, is provided with the interval [T of standard temperature in computer_L, T_H], when detection temperature >= T_H, computer controls heating film and stops heating；Temperature≤T when detection_L, computer controls heating film and begins to warm up.

The method of the knock-down gasifying device for elemene the most according to claim 5, is characterized in that, step (5-5) it After also comprise the steps:

WhenTime, the dose that Computer display suggestion is increased or decreased is 3% to 20%；

WhenTime, the dose that Computer display suggestion is increased or decreased is 20% to 35%；

WhenTime, the dose that Computer display suggestion is increased or decreased is more than 35%.

10., according to the method for the knock-down gasifying device for elemene described in claim 5 or 6 or 7 or 8, it is characterized in that, A1 Span be 0.14 to 0.32；The span of B1 is 0.2 to 0.28, and the span of B2 is 0.32 to 0.48.