CN105866217B - Harringtonine treatment of cancer effect detection device and method - Google Patents
Harringtonine treatment of cancer effect detection device and method Download PDFInfo
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- CN105866217B CN105866217B CN201610161241.3A CN201610161241A CN105866217B CN 105866217 B CN105866217 B CN 105866217B CN 201610161241 A CN201610161241 A CN 201610161241A CN 105866217 B CN105866217 B CN 105866217B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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Abstract
The invention discloses a kind of harringtonine treatment of cancer effect detection device and method, the present invention compares the signal-to-noise ratio peak of the harringtonine of unknown drug effect with the signal-to-noise ratio peak of conventional cancer treatment drugs cis-platinum, under the reference of the cis-platinum of clear and definite drug effect and dose, the chemotherapy effect and dosage of harringtonine are obtained.The present invention has that detection efficiency is high, accuracy is good, is used safely for harringtonine and provides reliable basis, it is ensured that the characteristics of patient safety.
Description
Technical field
The present invention relates to medicine effect detection technique field, more particularly, to a kind of cepehalotaxus fortunei that can accurately detect drug effect
Ester alkali treatment of cancer effect detection device and method.
Background technology
The dosage of cancer therapy drug must be accurate, when dose deficiency, can not reach therapeutic effect.When dose is beyond safety
Dosage, then it can cause the gastrointestinal reactions such as anorexia, nausea,vomiting,diarrhea.Common and serious toxic reaction is kidney poison
Property reaction, repeated drug taking can aggravate renal toxicity reaction, major determinant kidney proximal tubule, make Vacuole formation, exuviation, tube chamber
, there is hyalina, uric acid is excessive in blood, and the symptoms such as blood urine can be caused when serious in expansion.
When heavy dose and repeatedly medication, it may appear that neurotoxicity, the hair cell that ear Ke replaces mouthpart can be damaged, cause height
Frequency, which loses, to be listened.
The drug effect of PTS does not have clinical data may be referred to, and initial stage gropes to judge in use, fully relying on doctor,
If during dose deficiency, therapeutic effect is not reached；If dose can cause serious toxic reaction beyond safe dose, it can give and suffer from
Person's body brings very major injury.
The content of the invention
The goal of the invention of the present invention is to overcome the shortcomings of that the drug effect of PTS of the prior art is not clear, there is provided
A kind of harringtonine treatment of cancer effect detection device and method that can accurately detect drug effect.
To achieve these goals, the present invention uses following technical scheme：
A kind of harringtonine treatment of cancer effect detection device, including operating platform, the electrification on operating platform
Learn work station, 3 flow cells, the first solution bottle for filling cleaning solution and the second solution bottle for filling harringtonine solution；
Substrate is equipped with each flow cell, electrode chip is equipped with each substrate；The cover plate of first solution bottle and the second solution bottle
On be equipped with catheter, the catheter of the second solution bottle is provided with the first micro pump, and the catheter of the first solution bottle is provided with the
The liquid outlet of two micro pumps, the first micro pump and the second micro pump is connected with collector tube；Collector tube is provided with 3 catheters,
Each electrode chip with electrochemical workstation including being connected to electrode, reference electrode and working electrode, each working electrode
On culture have several cancer cells, each drain pipe is respectively protruding into the working electrode outer edge of 3 flow cells, under flow cell
Portion is provided with drain pipe；The catheter outer peripheral face is provided with heating film；Electrochemical workstation, the first micro pump, the second micro pump
With heating film with calculating mechatronics.
When harringtonine solution is dripped on cancer cell, cancer cell can react, what electrochemical workstation detected
Respective change can occur for current density signal, and detection signal Spect (t) inputs of detected new drug are pre-stored in meter by the present invention
In two layers of stochastic resonance system in calculation machine, computer draws the signal to noise ratio spectrogram of excitation noise signal, in signal to noise ratio spectrogram
The signal-to-noise ratio peak of the characteristic peak close to origin is chosen, and by the coordinate (M of signal-to-noise ratio peak1, M2) storage is into computer, meter
Characteristic peaks coordinate (the W of cis-platinum is stored with calculation machine1, W2)；
Work as M1＜ W1And | M2| ＞ | W2| when, Computer display harringtonine compares cis-platinum to the chemotherapy effect of cancer cell
Good, the dosage of harringtonine needs to reduce than cis-platinum；
When | M2| ＜ | W2| and M1＞ W1When, Computer display harringtonine compares cis-platinum to the chemotherapy effect of cancer cell
Difference, the dosage of harringtonine are needed than cis-platinum increase.
The signal-to-noise ratio peak and the letter of conventional cancer treatment drugs cis-platinum of harringtonine of the invention by unknown drug effect
Make an uproar and compared than peak value, under the reference of the cis-platinum of clear and definite drug effect and dose, obtain the drug effect and dose of harringtonine, be
Harringtonine safe handling provides reliable basis, it is ensured that the safety of patient.
Preferably, 2 vertical slots on operating platform, top plate and two, the cylinder being connected with top plate are equipped with each vertical slot
Cylinder is with calculating mechatronics；First solution bottle and the second solution bottle are located on two top plates respectively.
Two cylinders are used to driving top plate and solution bottle lifting moving thereon, so as to adjust the height of two micro pumps,
Reach the purpose of regulation liquid droping speed.
Preferably, being equipped with vibrator on the first solution bottle and the second solution bottle, two vibrators are with calculating electromechanics
Connection；Catheter is provided with temperature sensor, and temperature sensor is with calculating mechatronics；Level sensing is provided with first solution bottle
Device, liquid level sensor is with calculating mechatronics.
Preferably, each flow cell housing including upper end open, the cover plate on housing and on cover plate
Sealing ring, sealing ring lower surface is connected with to the base plate seals on the outside of electrode, working electrode and on the substrate between electrode
Provided with through hole.
A kind of method of harringtonine treatment of cancer effect detection device, comprises the following steps：
(5-1) computer controls heating film electrified regulation, and the first micro pump drips cleaning solution, and cleaning solution flows through drain
It is heated during pipe, for the first micro pump dropping liquid after 15 to 25 minutes, computer controls the first micro pump to stop dropping liquid；
(5-2) computer controls drip harringtonine solution, after 15 to 20 minutes, computer acquisition electrochemical workstation
3 current density signal S of detection1(t), S2(t), S3(t) normalised current density signal f (t), computer, are provided with computer
S is calculated respectively1(t), S2(t), S3(t) with f (t) error, it is current density signal S (t) to choose the wherein small definition of error；
Computer controls the electric current of second micro pump the second micro pump dropping liquid computer acquisition electrochemical workstation detection close
Signal S (t) is spent, computer chooses the sampled value that several time intervals are Δ t, each sampling in current density signal S (t)
Value is arranged to make up detection signal ES (t) according to time order and function order；
(5-3) is for first sampled value in ES (t) and each sampled value ES (t outside last sampled value1), profit
Use formulaCalculate steady coefficient ratio；
The weight threshold 0.55,1 and 1.45 increased successively is previously provided with computer；
It is located at the sampled value in the range of [1-A1,1+A1] for ratio, sampled value is modified to B1 ES (t1), B1 is small
In 0.4 real number；
It is located at the sampled value in the range of (0.55,1-A1) or (1+A1,1.45) for ratio, sampled value is modified to B2
ES(t1), B1 ＜ B2 ＜ 0.6；
(5-4) replaces the corresponding sampled value in ES (t) with corrected each sampled value, obtains the detection letter by amendment
Number Spect (t),
In two layers of stochastic resonance system that Spect (t) inputs are pre-stored in computer, computer draws excitation noise letter
Number signal to noise ratio spectrogram, the signal-to-noise ratio peak of the characteristic peak close to origin is chosen in signal to noise ratio spectrogram, and by signal-to-noise ratio peak
Coordinate (M1, M2) store the characteristic peaks coordinate (W that cis-platinum is stored with into computer, in computer1, W2)；
(5-5) works as M1＜ W1And | M2| ＞ | W2| when, chemotherapy effect ratio of the Computer display harringtonine to cancer cell
Cis-platinum is good, and the dosage of harringtonine needs to reduce than cis-platinum；
When | M2| ＜ | W2| and M1＞ W1When, Computer display harringtonine compares cis-platinum to the chemotherapy effect of cancer cell
Difference, the dosage of harringtonine are needed than cis-platinum increase.
Preferably, in two layers of stochastic resonance system that Spect (t) inputs are pre-stored in computer, computer
The signal to noise ratio spectrogram for drawing excitation noise signal comprises the following steps：
Spect (t) is inputted one layer of accidental resonance model by computer
In, wherein, V (x, t) is potential function, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are setting
Constant, ξ (t) are outer noises, and D is outer noise intensity, and N (t) grasps noise to be interior,For periodic sinusoidal signal, A
It is signal amplitude, f is signal frequency, and t is run duration,For phase, if
cx2-dx4To demarcate component；
Computer calculate V (x, t) for x first derivative and second dervative, and make equation be equal to 0, obtain two layers with
Machine resonance model：
Noise intensity D=0 is set,Spect (t)=0, N (t)=0；A critical value is calculated
For
A critical value is substituted into one layer of accidental resonance model, and sets X0(t)=0, sn0=0, with quadravalence jade for asking rain Ge Kuta
One layer of accidental resonance model of Algorithm for Solving, is obtained
And calculate
Wherein, xn(t) the n order derivatives for being x (t), snn-1It is value of S (t) the n-1 order derivatives at t=0, snn+1It is S
(t) value of the n+1 order derivatives at t=0, n=0,1 ..., N-1；Obtain x1(t), x2(t) ..., xn+1(t) value；
Computer is to x1(t), x2(t) ..., xn+1(t) integrated, obtain x (t), and it is random common at one layer to obtain x (t)
Two layers of stochastic resonance system that model and two layers of accidental resonance model form of shaking produce the position x at accidental resonance momentmValue and xm
Corresponding resonance moment t1And and t1Corresponding noise D1, D1For a value in D；
Computer utilizes formulaCalculate the letter of two layers of stochastic resonance system output
Make an uproar ratio；Wherein, Δ U=a2/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal.
Preferably, being equipped with vibrator on the first solution bottle and the second solution bottle, two vibrators are with calculating electromechanics
Connection；
Also comprise the following steps before step (5-1)：
Computer controls the vibrator works on the first solution bottle to stop vibration after 8 to 15 minutes；
Also comprise the following steps after step (5-1)：
Computer controls the vibrator works on the second solution bottle to stop vibration after 8 to 15 minutes.
Preferably, catheter is provided with temperature sensor, temperature sensor is with calculating mechatronics；Also include following step
Suddenly：
Temperature sensor detects the temperature of catheter, and normal temperature section [T is provided with computerL, TH], when the temperature of detection
Degree >=TH, computer control heating film stopping heating；As temperature≤T of detectionL, computer control heating film begin to warm up.
Preferably, being provided with liquid level sensor in the first solution bottle, liquid level sensor is with calculating mechatronics；Its feature
It is that liquid level sensor detects the liquid level in the first solution bottle, is provided with standard value P in computer, when the level value of detection is less than P,
The warning message that solution in the solution bottle of Computer display first requires supplementation with.
Preferably, the value model that the span that A1 span is 0.18 to 0.31, B1 is 0.19 to 0.28, B2
Enclose for 0.28 to 0.52.
Therefore, the present invention has the advantages that：Detection efficiency is high, accuracy is good, is used safely for harringtonine
Provide reliable basis, it is ensured that the safety of patient.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the present invention；
Fig. 2 is a kind of structural representation of the groove of the present invention；
Fig. 3 is a kind of theory diagram of the present invention；
Fig. 4 is a kind of structural representation of the electrode chip of the present invention；
Fig. 5 is a kind of flow chart of embodiments of the invention.
Fig. 6 is a kind of signal to noise ratio spectrogram of the cis-platinum of the present invention.
In figure：Operating platform 1, electrochemical workstation 2, flow cell 3, the first solution bottle 4, the second solution bottle 5, substrate 6, electricity
Pole piece piece 7, collector tube 8, the first micro pump 9, the second micro pump 10, catheter 11, computer 12, vibrator 13, TEMP
Device 14, liquid level sensor 15, drain pipe 31, through hole 61, painting layer 62, to electrode 71, reference electrode 72, working electrode 73,
Vertical slot 101, top plate 102, cylinder 103, heating film 112.
Embodiment
The present invention will be further described with reference to the accompanying drawings and detailed description.
Embodiment as shown in Figure 1, Figure 3 is a kind of harringtonine treatment of cancer effect detection device, including operation is flat
Platform 1,2,3 flow cells 3 of electrochemical workstation on operating platform, fill the first solution bottle 4 of cleaning solution and fill
Second solution bottle 5 of harringtonine solution；Substrate 6 is equipped with each flow cell, electrode chip is equipped with each substrate
7；Catheter is equipped with the cover plate of first solution bottle and the second solution bottle, the catheter of the second solution bottle is micro provided with first
Pump 9, the catheter of the first solution bottle are provided with the second micro pump 10, the liquid outlet of the first micro pump and the second micro pump with collection
Liquid pipe 8 connects；Collector tube is provided with 3 catheters 11, and each drain pipe is respectively protruding into the working electrode outward flange of 3 flow cells
Place, flow cell bottom is provided with drain pipe 31；Catheter outer peripheral face is provided with heating film 112.
As shown in figure 3, electrochemical workstation, the first micro pump, the second micro pump and heating film are electrically connected with computer 12
Connect.Each substrate is made of ag material.
As shown in figure 4, each electrode chip include being connected with electrochemical workstation to electrode 71, reference electrode 72 and
Working electrode 73, culture has 25000 cancer cells on each working electrode.
As shown in Fig. 22 vertical slots 101 on operating platform, top plate 102 and cylinder 103, cylinder are equipped with each vertical slot
Expansion link be connected with top plate lower surface, two cylinders with calculate mechatronics；First solution bottle and the second solution bottle difference
On two top plates.
As shown in figure 3, be equipped with vibrator 13 on the first solution bottle and the second solution bottle, two vibrators are and computer
Electrical connection；Catheter is provided with temperature sensor 14, and temperature sensor is with calculating mechatronics；Liquid level is provided with first solution bottle
Sensor 15, liquid level sensor is with calculating mechatronics.
As shown in figure 4, each flow cell housing including upper end open, the cover plate on housing and on cover plate
Sealing ring, sealing ring lower surface is connected with to the base plate seals on the outside of electrode, working electrode and on the substrate between electrode
Provided with through hole 61.
As shown in figure 5, a kind of method of harringtonine treatment of cancer effect detection device, comprises the following steps：
Step 100, preheat, clean
Computer controls the vibrator works on the first solution bottle to stop vibration after 10 minutes；
Temperature sensor detects the temperature of catheter, and normal temperature section [T is provided with computerL, TH], when the temperature of detection
Degree >=TH, computer control heating film stopping heating；As temperature≤T of detectionL, computer control heating film begin to warm up.TL=
36.0 DEG C, TH=39.6 DEG C；
First micro pump is dripped cleaning solution, and cleaning solution is heated when flowing through catheter, and the first micro pump dropping liquid 25 divides
Zhong Hou, computer control the first micro pump to stop dropping liquid；Computer controlled the vibrator works on the second solution bottle after 10 minutes
Stop vibration；Cleaning solution is phosphate buffer.
Step 200, detect
Computer controls the harringtonine solution that drips, after 20 minutes, 3 of the detection of computer acquisition electrochemical workstation
Current density signal S1(t), S2(t), S3(t) normalised current density signal f (t), is provided with computer, computer calculates respectively
S1(t), S2(t), S3(t) with f (t) error, it is current density signal S (t) to choose the wherein small definition of error；
Computer controls the electric current of second micro pump the second micro pump dropping liquid computer acquisition electrochemical workstation detection close
Signal S (t) is spent, computer chooses the sampled value that 1000 time intervals are Δ t, each sampling in current density signal S (t)
Value is arranged to make up detection signal ES (t) according to time order and function order；
Step 300, data correction is handled
For first sampled value in ES (t) and each sampled value ES (t outside last sampled value1), utilize public affairs
FormulaCalculate steady coefficient ratio；
The weight threshold 0.55,1 and 1.45 increased successively is previously provided with computer；
It is located at the sampled value in the range of [1-A1,1+A1] for ratio, sampled value is modified to B1 ES (t1), A1=
0.25, B1 0.2；
It is located at the sampled value in the range of (0.55,1-A1) or (1+A1,1.45) for ratio, sampled value is modified to B2
ES(t1), B2 0.4；
Step 400, the signal-to-noise ratio peak at selected characteristic peak
The corresponding sampled value in ES (t) is replaced with corrected each sampled value, obtains the detection signal by amendment
Spect (t),
Spect (t) is inputted one layer of accidental resonance model by computer
In, wherein, V (x, t) is potential function, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are setting
Constant, ξ (t) are outer noises, and D is outer noise intensity, and N (t) grasps noise to be interior,For periodic sinusoidal signal, A
It is signal amplitude, f is signal frequency, and t is run duration,For phase, if
cx2-dx4To demarcate component；
Computer calculate V (x, t) for x first derivative and second dervative, and make equation be equal to 0, obtain two layers with
Machine resonance model：
Noise intensity D=0 is set,Spect (t)=0, N (t)=0；A critical value is calculated
For
A critical value is substituted into one layer of accidental resonance model, and sets X0(t)=0, sn0=0, with quadravalence jade for asking rain Ge Kuta algorithms
One layer of accidental resonance model is solved, is obtained
And calculate
Wherein, xn(t) the n order derivatives for being x (t), snn-1It is value of S (t) the n-1 order derivatives at t=0, snn+1It is S
(t) value of the n+1 order derivatives at t=0, n=0,1 ..., N-1；Obtain x1(t), x2(t) ..., xn+1(t) value；
Computer is to x1(t), x2(t) ..., xn+1(t) integrated, obtain x (t), and it is random common at one layer to obtain x (t)
Two layers of stochastic resonance system that model and two layers of accidental resonance model form of shaking produce the position x at accidental resonance momentmValue and xm
Corresponding resonance moment t1And and t1Corresponding noise D1, D1For a value in D；
Computer utilizes formulaCalculate the letter of two layers of stochastic resonance system output
Make an uproar ratio；Wherein, Δ U=a2/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal；
The signal-to-noise ratio peak of the characteristic peak close to origin is chosen in signal to noise ratio spectrogram, and by the coordinate of signal-to-noise ratio peak
(M1, M2) store the characteristic peaks coordinate (W that cis-platinum is stored with into computer, in computer1, W2)；The characteristic peaks of cis-platinum are sat
Mark (W1, W2) equally it is to be obtained using the method for step 100 to 400, the cisplatin solution being contained in respectively in 2 the second solution bottles
It is identical with harringtonine concentration.
Step 500, make chemotherapy effect and dose judges
Work as M1＜ W1And | M2| ＞ | W2| when, Computer display harringtonine compares cis-platinum to the chemotherapy effect of cancer cell
Good, the dosage of harringtonine needs to reduce than cis-platinum；
When | M2| ＜ | W2| and M1＞ W1When, Computer display harringtonine compares cis-platinum to the chemotherapy effect of cancer cell
Difference, the dosage of harringtonine are needed than cis-platinum increase.
As shown in fig. 6, the signal-to-noise ratio peak of the characteristic peak of cis-platinum is (20, -34dB), the spy of harringtonine of the invention
The signal-to-noise ratio peak for levying peak is (14, -53dB)；
Then Computer display harringtonine is better than cis-platinum to the chemotherapy effect of cancer cell, and the dosage of harringtonine needs
Than the information of cis-platinum reduction.
Additionally include following steps：
Liquid level sensor detects the liquid level in the first solution bottle, standard value P is provided with computer, when the level value of detection is small
The warning message that solution in P, the solution bottle of Computer display first requires supplementation with.
It should be understood that the present embodiment is only illustrative of the invention and is not intended to limit the scope of the invention.In addition, it is to be understood that
After having read the content of the invention lectured, those skilled in the art can make various changes or modifications to the present invention, these etc.
Valency form equally falls within the application appended claims limited range.
Claims (9)
1. a kind of method for harringtonine treatment of cancer effect detection device, harringtonine treatment of cancer effect detection
Device includes operating platform (1), electrochemical workstation (2), 3 flow cells (3) on operating platform, fills cleaning solution
The first solution bottle (4) and fill the second solution bottle (5) of harringtonine solution；Substrate (6) is equipped with each flow cell,
Electrode chip (7) is equipped with each substrate；Catheter is equipped with the cover plate of first solution bottle and the second solution bottle, second is molten
The catheter of liquid bottle is provided with the first micro pump (9), and the catheter of the first solution bottle is provided with the second micro pump (10), and first is micro-
The liquid outlet of amount pump and the second micro pump is connected with collector tube (8)；Collector tube is provided with 3 catheters (11), each electrode
Chip with electrochemical workstation including being connected to electrode (71), reference electrode (72) and working electrode (73), each work
Culture has several cancer cells on electrode, and each drain pipe is respectively protruding into the working electrode outer edge of 3 flow cells, circulation
Pond bottom is provided with drain pipe (31)；The catheter outer peripheral face is provided with heating film (112)；It is electrochemical workstation, first micro
Pump, the second micro pump and heating film electrically connect with computer (12)；It is characterized in that comprise the following steps：
(1-1) computer controls heating film electrified regulation, and the first micro pump drips cleaning solution, when cleaning solution flows through catheter
It is heated, for the first micro pump dropping liquid after 15 to 25 minutes, computer controls the first micro pump to stop dropping liquid；
(1-2) computer controls the harringtonine solution that drips, after 15 to 20 minutes, the detection of computer acquisition electrochemical workstation
3 current density signal S1(t), S2(t), S3(t) normalised current density signal f (t), computer difference, are provided with computer
Calculate S1(t), S2(t), S3(t) with f (t) error, it is current density signal S (t) to choose the wherein small definition of error；
Computer controls the current density letter of second micro pump the second micro pump dropping liquid computer acquisition electrochemical workstation detection
Number S (t), computer choose the sampled value that several time intervals are Δ t in current density signal S (t), and each sampled value is pressed
Detection signal ES (t) is arranged to make up according to time order and function order；
(1-3) is for first sampled value in ES (t) and each sampled value ES (t outside last sampled value1), utilize public affairs
FormulaCalculate steady coefficient ratio；
The weight threshold 0.55,1 and 1.45 increased successively is previously provided with computer；
It is located at the sampled value in the range of [1-A1,1+A1] for ratio, sampled value is modified to B1ES (t1), B1 is less than 0.4
Real number；
It is located at the sampled value in the range of (0.55,1-A1) or (1+A1,1.45) for ratio, sampled value is modified to B2ES
(t1), B1 p B2 ＜ 0.6；
(1-4) replaces the corresponding sampled value in ES (t) with corrected each sampled value, obtains the detection signal by amendment
Spect (t),
In two layers of stochastic resonance system that Spect (t) inputs are pre-stored in computer, computer draws excitation noise signal
Signal to noise ratio spectrogram, the signal-to-noise ratio peak of the characteristic peak close to origin is chosen in signal to noise ratio spectrogram, and by the seat of signal-to-noise ratio peak
Mark (M1, M2) store the characteristic peaks coordinate (W that cis-platinum is stored with into computer, in computer1, W2)；
(1-5) works as M1 p W1And | M2|f|W2| when, Computer display harringtonine compares cis-platinum to the chemotherapy effect of cancer cell
Good, the dosage of harringtonine needs to reduce than cis-platinum；
When | M2|p|W2| and M1 f W1When, Computer display harringtonine is poorer than cis-platinum to the chemotherapy effect of cancer cell, and three
The dosage of sharp China fir ester alkali is needed than cis-platinum increase.
2. the method according to claim 1 for harringtonine treatment of cancer effect detection device, it is characterized in that, behaviour
Make 2 vertical slots (101) on platform, top plate (102) and the cylinder (103) being connected with top plate, two gas are equipped with each vertical slot
Cylinder is with calculating mechatronics；First solution bottle and the second solution bottle are located on two top plates respectively.
3. the method according to claim 1 for harringtonine treatment of cancer effect detection device, it is characterized in that, the
Vibrator (13) is equipped with one solution bottle and the second solution bottle, two vibrators are with calculating mechatronics；Set on catheter
There is temperature sensor (14), temperature sensor is with calculating mechatronics；Liquid level sensor (15), liquid level are provided with first solution bottle
Sensor is with calculating mechatronics.
4. the method according to claim 1 for harringtonine treatment of cancer effect detection device, it is characterized in that, often
Individual flow cell includes housing, the cover plate on housing and the sealing ring on cover plate of upper end open, sealing ring following table
Face is connected with to the base plate seals on the outside of electrode, working electrode and is provided with through hole (61) to the substrate between electrode.
5. the method according to claim 1 for harringtonine treatment of cancer effect detection device, it is characterized in that,
In two layers of stochastic resonance system that Spect (t) inputs are pre-stored in computer, computer draws excitation noise letter
Number signal to noise ratio spectrogram comprise the following steps：
Spect (t) is inputted one layer of accidental resonance model by computer
In, wherein, V (x, t) is potential function, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are the normal of setting
Number, ξ (t) are outer noises, and D is outer noise intensity, and N (t) grasps noise to be interior,For periodic sinusoidal signal, A is
Signal amplitude, f are signal frequencies, and t is run duration,For phase, if
cx2-dx4To demarcate component；
Computer calculates first derivative and second dervative of the V (x, t) for x, and equation is equal to 0, obtains two layers at random altogether
Shake model：
Noise intensity D=0 is set,Spect (t)=0, N (t)=0；The critical value that A is calculated is
A critical value is substituted into one layer of accidental resonance model, and sets X0(t)=0, sn0=0, asked with quadravalence jade for asking rain Ge Kuta algorithms
One layer of accidental resonance model is solved, is obtainedAnd
Calculate
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Wherein, xn(t) the n order derivatives for being x (t), snn-1It is value of S (t) the n-1 order derivatives at t=0, snn+1It is S (t) n+
Value of 1 order derivative at t=0, n=0,1 ..., N-1；Obtain x1(t), x2(t), L, xn+1(t) value；
Computer is to x1(t), x2(t), L, xn+1(t) integrated, obtain x (t), and obtain x (t) in one layer of accidental resonance model
The position x at accidental resonance moment is produced with two layers of stochastic resonance system of two layers of accidental resonance model compositionmValue and xmIt is corresponding
Resonance moment t1And and t1Corresponding noise D1, D1For a value in D；
Computer utilizes formulaCalculate the signal to noise ratio of two layers of stochastic resonance system output；
Wherein, Δ U=a2/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal.
6. the method according to claim 1 for harringtonine treatment of cancer effect detection device, the first solution bottle
Be equipped with vibrator on the second solution bottle, two vibrators are with calculating mechatronics；It is characterized in that
Also comprise the following steps before step (1-1)：
Computer controls the vibrator works on the first solution bottle to stop vibration after 8 to 15 minutes；
Also comprise the following steps after step (1-1)：
Computer controls the vibrator works on the second solution bottle to stop vibration after 8 to 15 minutes.
7. the method according to claim 1 for harringtonine treatment of cancer effect detection device, set on catheter
There is temperature sensor, temperature sensor is with calculating mechatronics；It is characterized in that also comprise the following steps：
Temperature sensor detects the temperature of catheter, and normal temperature section [T is provided with computerL, TH], when detection temperature >=
TH, computer control heating film stopping heating；As temperature≤T of detectionL, computer control heating film begin to warm up.
8. it is used for harringtonine treatment of cancer effect detection device according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7
Method, liquid level sensor is provided with the first solution bottle, liquid level sensor is with calculating mechatronics；It is characterized in that level sensing
Device detects the liquid level in the first solution bottle, is provided with standard value P in computer, when the level value of detection is less than P, Computer display the
The warning message that solution in one solution bottle requires supplementation with.
9. it is used for harringtonine treatment of cancer effect detection device according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7
Method, it is characterized in that, the value model that the span that A1 span is 0.18 to 0.31, B1 is 0.19 to 0.28, B2
Enclose for 0.28 to 0.52.
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