CN105886478A - Composite enzyme preparation for resourceful treatment of sludge and preparing method of composite enzyme preparation - Google Patents
Composite enzyme preparation for resourceful treatment of sludge and preparing method of composite enzyme preparation Download PDFInfo
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- CN105886478A CN105886478A CN201610280269.9A CN201610280269A CN105886478A CN 105886478 A CN105886478 A CN 105886478A CN 201610280269 A CN201610280269 A CN 201610280269A CN 105886478 A CN105886478 A CN 105886478A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/12—Treatment of sludge; Devices therefor by de-watering, drying or thickening
- C02F11/14—Treatment of sludge; Devices therefor by de-watering, drying or thickening with addition of chemical agents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention relates to a composite enzyme preparation for the resourceful treatment of sludge and a preparing method of the composite enzyme preparation, belongs to the field of sludge treatment, and aims to overcome the defects in the prior art. The composite enzyme preparation has the advantages that the composite enzyme preparation is purely prepared by natural plants through one-step fermentation, contains at least 25 enzymes and thousands of sub-enzymes and can bring the optimal benefit into play under various environments; the sludge can be deodorized by adding the composite enzyme preparation, a large amount organic matter in the sludge is degraded after the sludge is pretreated by the composite enzyme, and clear water and bottom sludge are completely separated.
Description
Technical field
The present invention relates to a kind of compound enzymic preparation for reuse of dredging process and preparation method thereof, belong to mud
Process field.
Background technology
China's medium and small river channel sedimentation phenomenon is commonplace, and the original flood of regulating and storing in river course has with the ability prevented and reduced natural disasters
Weakened.Country strengthened middle river channel and the management in river course, rural area, the conduct of wherein desilting engineering in recent years
Major measure is widely implemented.At present channel cleanout from the past only to improve river flood control, drain flooded fields and fill
The traditional water conservancy engineering objective irrigating ability is expanded to Ecological waterscape target, say, that in a lot of channel cleanout engineerings
Target in all contain and be reduced river course endogenous pollution, improve the engineering purpose provided safeguard for river water quality.Such as,
If river is gushed dredging silt and can not be processed in time and will give out stench, cause the biggest ecological pressure to surrounding
Power.
River is gushed dredging silt foul smell and is mostly derived from river to gush bed mud content of organics too high, and Organic substance consumes bed mud oxygen,
Reduce bed mud oxidation-reduction potential, make the anaerobe amount reproduction in bed mud and water body, will bed mud have
Machine thing is decomposed into methane, hydrogen sulfide, ammonia nitrogen and pyrimidine etc., and wherein hydrogen sulfide, ammonia nitrogen and pyrimidine are to have stench
The material of character, under anaerobic condition, river bottom mud and water body high volence metal ion such as Fe and Mn etc. are gone back simultaneously
Originally it was low price ion such as Fe2+、Mn2+Deng, with water body present in sulfur generation chemical reaction, formed stable black
Color substance such as FeS and MnS etc., thus cause bed mud black smelly.
The best approach removing bed mud stench is carried out a biological disposal upon exactly.The most state-of-the-art biological deodorizing technology at present
It is to utilize compound enzyme to carry out decomposing organic matter.Due in mud except containing in addition to organic, there is also substantial amounts of micro-life
Thing.These microorganism great majority all can actively adsorb Organic substance about, but is under anaerobic difficult to breeding.
Enzyme preparation is the strongest catalyst, and compared with organic catalyst, the organic speed of enzymatic degradation to exceed thousand
More than ten thousand times.
CN103420545B discloses a kind of Sludge digestion method based on microbial compound enzyme, micro-life therein
Thing enzymatic catalysts includes Fructus Ananadis comosi fibroin enzyme, free shape ferric iron, and nicotinamide adenine dinucleotide is mixed
Close, then be mixed to prepare in the range of accommodation that pH is 10 with yeast leachate, but enzyme contained therein
The most single, satisfactory to the degree for the treatment of difficulty of mud.It is clear that CN102276124B discloses a kind of microorganism
Silt agent, it is with porous zeolite granule as carrier, by actinomycetes, Europe thin, the Vickers nitrobacteria of nitrification monospore etc.
Antibacterial forms, wherein contain multiple can be with the antibacterial of digested sludge, but it is due to the environment of mud and antibacterial
Composition, cause its digestive efficiency relatively low.
Summary of the invention
In order to overcome the deficiencies in the prior art, the present invention provides a kind of compound enzyme system processed for reuse of dredging
Agent and preparation method thereof, described compound enzymic preparation 100% natural plants one time fermentation forms, containing at least 25
Plant above enzyme and thousands of seed enzyme, optimum efficiency can be played under circumstances.Add compound enzymic preparation not
Only and to mud carrying out deodorization, meanwhile, after compound enzyme pretreatment, the Organic substance in mud is degraded in a large number,
Clear water and bed mud are kept completely separate.
The present invention solves above-mentioned technical problem by following technical proposals:
A kind of compound enzymic preparation processed for reuse of dredging, it uses following preparation technology to prepare:
1) fermentation medium preparation: prepare fermentation medium according to following formula: the component of fermentation medium is Portugal
Grape sugar 10g, K2HPO42g, KH2PO42g, MgSO4·7H2O 0.2g, CaCl20.1g, carbamide 0.5g,
Yeast extract 0.5g, Chinese medicine extraction extractum 10g, pH7.2-7.5,120 DEG C sterilizing 30min, wherein Chinese medicine extraction
The preparation method of extractum is broken into coarse powder for taking recipe quantity natural plants, according to 4～9 times of additions of coarse powder gross weight
Volumetric concentration is the ethanol solution of 40%～95%, reflux, extract, three times, and return time is 2～5h, filters,
Filtrate recycling ethanol, cold filtration, wash with water, the most i.e. obtain Chinese medical concrete, described natural plants by
Alfalfa, schefflera, Bulbus Lilii and Caulis Hederae Sinensis composition.Preferably, described natural plants is by following weight portion
Component composition: alfalfa 35-45 part, schefflera 23-27 part, Bulbus Lilii 31-39 part, Caulis Hederae Sinensis 8-12
Part, it is further preferred that described natural plants is made up of the component of following weight portion: alfalfa 40 parts,
Schefflera 25 parts, Bulbus Lilii 35 parts, Caulis Hederae Sinensis 10 parts；
2) biofermentation: after fermentation medium sterilizing, accesses the Rhodococcus coprophilus seed fermentation liquid of 10%, control
Fermentation temperature processed is 30-32 DEG C, ventilation is 1.5-1.7m3Cultivate 40-44h under/h, now access 10%
Aspergillus oryzae seed fermentation liquid, control fermentation temperature be 26-29 DEG C, ventilation be 1.8-2.2m3Train under/h
Support 26-30h, obtain containing enzyme fermentation liquid；
3) enzyme preparation is extracted: regulate to fermentation liquid to containing the Tris-HCl solution adding 5 times of volumes in enzyme fermentation liquid
PH be between 5.4-6.2, ultrasonic 3-5min at 4 DEG C, 4 DEG C of centrifuging and taking supernatant of refrigerated centrifuger, Xiang Qi
The pre-cold acetone of middle 4 times of volumes of addition ,-20 DEG C stand frozen centrifugation after 16-18 hour, take precipitation use third
After ketone washes twice, obtain compound enzymic preparation.
Above-mentioned in the compound enzymic preparation that reuse of dredging processes, described Rhodococcus coprophilus and aspergillus oryzae access
Being in exponential phase before fermentation liquid, its colony density is no less than 2 × 109cfu/mL。
Compound enzymic preparation described above can apply to the recycling treatment of mud.Its concrete recycling treatment
Method is:
In mud, add compound enzymic preparation carry out anaerobic digestion, control the temperature of mud between 26-28 DEG C,
The process time is 3-6 hour, and the addition of described compound enzymic preparation is 5-15ppm (weight ratio), then
Mud natural subsidence is air-dried.Preferably, the described process time is 4 hours, described compound enzyme system
The addition of agent is 10ppm.
Larger molecular organics in mud can be converted into by the compound enzymic preparation (exoenzyme) added in this technique
The organic molecule of solubility, then small organic molecule penetrates into inside microbial cell by cell membrane, respectively
Planting under the effect of endoenzyme, through the enzyme reaction of series of complex, be then degraded, a part is converted into inorganic matter
And energy, a part is used for synthesizing Self substances by microorganism.For the Organic substance of part difficult degradation in bed mud,
Microorganism also can first change its structure, the most at last its degraded by the effect of " cometabolism ".
This technique use compound enzymic preparation 100% form by natural plants one time fermentation, containing at least 25 kinds with
On enzyme and thousands of seed enzyme, be wide spectrum compound enzyme truly, can play optimal under circumstances
Benefit.The bed mud gushed such as Shahe, adds the compound enzyme of 10ppm, and aeration 4 hours, stench removes entirely.Add
Add compound enzymic preparation and not only and mud is carried out deodorization, meanwhile, after compound enzyme pretreatment, in mud
Organic substance is degraded in a large number, and clear water and bed mud are kept completely separate.The physical property of bed mud there occurs change, it is easier to de-
Water and mummification.
Detailed description of the invention
The present invention is further described below by way of specific embodiment, but described embodiment limiting never in any form
Determine the scope of patent protection of the present invention.
Compound enzymic preparation that embodiment 1 present invention processes for reuse of dredging and preparation method thereof
A kind of compound enzymic preparation processed for reuse of dredging, it uses following preparation technology to prepare:
1) fermentation medium preparation: prepare fermentation medium according to following formula: the component of fermentation medium is
Glucose 10g, K2HPO42g, KH2PO42g, MgSO4·7H2O 0.2g, CaCl20.1g, carbamide 0.5g,
Yeast extract 0.5g, Chinese medicine extraction extractum 10g, pH7.2-7.5,120 DEG C sterilizing 30min, wherein Chinese medicine extraction
The preparation method of extractum is broken into coarse powder for taking recipe quantity natural plants, according to 4 times of addition volumes of coarse powder gross weight
Concentration is the ethanol solution of 40%～95%, reflux, extract, three times, and return time is 4h, filters, and filtrate is returned
Receiving ethanol, cold filtration, wash with water, the most i.e. obtain Chinese medical concrete, described natural plants is by weighing as follows
The component composition of amount part: alfalfa 35 parts, schefflera 23 parts, Bulbus Lilii 31 parts, Caulis Hederae Sinensis 8 parts；
2) biofermentation: after fermentation medium sterilizing, accesses the Rhodococcus coprophilus seed fermentation liquid of 10%, control
Fermentation temperature processed is 30 DEG C, ventilation is 1.5m3Cultivate 40h under/h, now access the rice-koji of 10%
Mould seed fermentation liquid, control fermentation temperature be 26 DEG C, ventilation be 1.8m3Cultivate 26h under/h, to obtain final product
Containing enzyme fermentation liquid；
3) enzyme preparation is extracted: to containing adding the Tris-HCl solution regulation of 5 times of volumes in enzyme fermentation liquid to fermentation
The pH of liquid is ultrasonic 3min at 5.4,4 DEG C, 4 DEG C of centrifuging and taking supernatant of refrigerated centrifuger, is added thereto to 4
The pre-cold acetone of times volume ,-20 DEG C stand 16 hours after frozen centrifugation, take after precipitation uses washing with acetone twice,
Obtain compound enzymic preparation.
Compound enzymic preparation that embodiment 2 present invention processes for reuse of dredging and preparation method thereof
A kind of compound enzymic preparation processed for reuse of dredging, it uses following preparation technology to prepare:
1) fermentation medium preparation: prepare fermentation medium according to following formula: the component of fermentation medium is
Glucose 10g, K2HPO42g, KH2PO42g, MgSO4·7H2O 0.2g, CaCl20.1g, carbamide 0.5g,
Yeast extract 0.5g, Chinese medicine extraction extractum 10g, pH7.2-7.5,120 DEG C sterilizing 30min, wherein Chinese medicine extraction
The preparation method of extractum is broken into coarse powder for taking recipe quantity natural plants, according to 9 times of addition volumes of coarse powder gross weight
Concentration is the ethanol solution of 40%～95%, reflux, extract, three times, and return time is 5h, filters, and filtrate is returned
Receiving ethanol, cold filtration, wash with water, the most i.e. obtain Chinese medical concrete, described natural plants is by weighing as follows
The component composition of amount part: alfalfa 45 parts, schefflera 27 parts, Bulbus Lilii 39 parts, Caulis Hederae Sinensis 12 parts；
2) biofermentation: after fermentation medium sterilizing, accesses the Rhodococcus coprophilus seed fermentation liquid of 10%, control
Fermentation temperature processed is 32 DEG C, ventilation is 1.7m3Cultivate 44h under/h, now access the rice-koji of 10%
Mould seed fermentation liquid, control fermentation temperature be 29 DEG C, ventilation be 2.2m3Cultivate 30h under/h, to obtain final product
Containing enzyme fermentation liquid；
3) enzyme preparation is extracted: to containing adding the Tris-HCl solution regulation of 5 times of volumes in enzyme fermentation liquid to fermentation
The pH of liquid is ultrasonic 5min at 6.2,4 DEG C, 4 DEG C of centrifuging and taking supernatant of refrigerated centrifuger, is added thereto to 4
The pre-cold acetone of times volume ,-20 DEG C stand 18 hours after frozen centrifugation, take after precipitation uses washing with acetone twice,
Obtain compound enzymic preparation.
Compound enzymic preparation that embodiment 3 present invention processes for reuse of dredging and preparation method thereof
A kind of compound enzymic preparation processed for reuse of dredging, it uses following preparation technology to prepare:
1) fermentation medium preparation: prepare fermentation medium according to following formula: the component of fermentation medium is
Glucose 10g, K2HPO42g, KH2PO42g, MgSO4·7H2O 0.2g, CaCl20.1g, carbamide 0.5g,
Yeast extract 0.5g, Chinese medicine extraction extractum 10g, pH7.2-7.5,120 DEG C sterilizing 30min, wherein Chinese medicine extraction
The preparation method of extractum is broken into coarse powder for taking recipe quantity natural plants, according to 6 times of addition volumes of coarse powder gross weight
Concentration is the ethanol solution of 40%～95%, reflux, extract, three times, and return time is 4h, filters, and filtrate is returned
Receiving ethanol, cold filtration, wash with water, the most i.e. obtain Chinese medical concrete, described natural plants is by weighing as follows
The component composition of amount part: alfalfa 40 parts, schefflera 25 parts, Bulbus Lilii 35 parts, Caulis Hederae Sinensis 10 parts；
2) biofermentation: after fermentation medium sterilizing, accesses the Rhodococcus coprophilus seed fermentation liquid of 10%, control
Fermentation temperature processed is 32 DEG C, ventilation is 1.6m3Cultivate 42h under/h, now access the rice-koji of 10%
Mould seed fermentation liquid, control fermentation temperature be 28 DEG C, ventilation be 2.0m3Cultivate 28h under/h, to obtain final product
Containing enzyme fermentation liquid；
3) enzyme preparation is extracted: to containing adding the Tris-HCl solution regulation of 5 times of volumes in enzyme fermentation liquid to fermentation
The pH of liquid is between 5.8, ultrasonic 4min at 4 DEG C, 4 DEG C of centrifuging and taking supernatant of refrigerated centrifuger, adds wherein
Enter the pre-cold acetone of 4 times of volumes ,-20 DEG C stand 17 hours after frozen centrifugation, take precipitation and use washing with acetone two
After secondary, obtain compound enzymic preparation.
Compound enzymic preparation that embodiment 4 present invention processes for reuse of dredging and preparation method thereof
A kind of compound enzymic preparation processed for reuse of dredging, it uses following preparation technology to prepare:
1) fermentation medium preparation: prepare fermentation medium according to following formula: the component of fermentation medium is
Glucose 10g, K2HPO42g, KH2PO42g, MgSO4·7H2O 0.2g, CaCl20.1g, carbamide 0.5g,
Yeast extract 0.5g, Chinese medicine extraction extractum 10g, pH7.2-7.5,120 DEG C sterilizing 30min, wherein Chinese medicine extraction
The preparation method of extractum is broken into coarse powder for taking recipe quantity natural plants, according to 9 times of addition volumes of coarse powder gross weight
Concentration is the ethanol solution of 40%～95%, reflux, extract, three times, and return time is 2h, filters, and filtrate is returned
Receiving ethanol, cold filtration, wash with water, the most i.e. obtain Chinese medical concrete, described natural plants is by weighing as follows
The component composition of amount part: alfalfa 45 parts, schefflera 23 parts, Bulbus Lilii 39 parts, Caulis Hederae Sinensis 8 parts；
2) biofermentation: after fermentation medium sterilizing, accesses the Rhodococcus coprophilus seed fermentation liquid of 10%, control
Fermentation temperature processed is 32 DEG C, ventilation is 1.5m3Cultivate 44h under/h, now access the rice-koji of 10%
Mould seed fermentation liquid, control fermentation temperature be 26 DEG C, ventilation be 2.2m3Cultivate 26 under/h, to obtain final product
Containing enzyme fermentation liquid；
3) enzyme preparation is extracted: to containing adding the Tris-HCl solution regulation of 5 times of volumes in enzyme fermentation liquid to fermentation
The pH of liquid is ultrasonic 3min at 6.2,4 DEG C, 4 DEG C of centrifuging and taking supernatant of refrigerated centrifuger, is added thereto to 4
The pre-cold acetone of times volume ,-20 DEG C stand 18 hours after frozen centrifugation, take after precipitation uses washing with acetone twice,
Obtain compound enzymic preparation.
Compound enzymic preparation that embodiment 5 present invention processes for reuse of dredging and preparation method thereof
A kind of compound enzymic preparation processed for reuse of dredging, it uses following preparation technology to prepare:
1) fermentation medium preparation: prepare fermentation medium according to following formula: the component of fermentation medium is
Glucose 10g, K2HPO42g, KH2PO42g, MgSO4·7H2O 0.2g, CaCl20.1g, carbamide 0.5g,
Yeast extract 0.5g, Chinese medicine extraction extractum 10g, pH7.2-7.5,120 DEG C sterilizing 30min, wherein Chinese medicine extraction
The preparation method of extractum is broken into coarse powder for taking recipe quantity natural plants, according to 4～9 times of additions of coarse powder gross weight
Volumetric concentration is the ethanol solution of 40%～95%, reflux, extract, three times, and return time is 2～5h, filters,
Filtrate recycling ethanol, cold filtration, wash with water, the most i.e. obtain Chinese medical concrete, described natural plants by
The component composition of following weight portion: alfalfa 35 parts, schefflera 27 parts, Bulbus Lilii 31 parts, Caulis Hederae Sinensis 12
Part；
2) biofermentation: after fermentation medium sterilizing, accesses the Rhodococcus coprophilus seed fermentation liquid of 10%, control
Fermentation temperature processed is 30 DEG C, ventilation is 1.5m3Cultivate 44h under/h, now access the rice-koji of 10%
Mould seed fermentation liquid, control fermentation temperature be 26 DEG C, ventilation be 2.2m326-30h is cultivated, i.e. under/h
Enzyme fermentation liquid must be contained；
3) enzyme preparation is extracted: to containing adding the Tris-HCl solution regulation of 5 times of volumes in enzyme fermentation liquid to fermentation
The pH of liquid is ultrasonic 3min at 6.2,4 DEG C, 4 DEG C of centrifuging and taking supernatant of refrigerated centrifuger, is added thereto to 4
The pre-cold acetone of times volume ,-20 DEG C stand 18 hours after frozen centrifugation, take after precipitation uses washing with acetone twice,
Obtain compound enzymic preparation.
Compound enzymic preparation described in embodiment 6 is applied to the treatment effect of the recycling treatment of mud
Described compound enzymic preparation can apply to the recycling processing method of the recycling treatment of mud: to silt
Adding compound enzymic preparation in mud and carry out anaerobic digestion, the temperature of control mud, between 26-28 DEG C, processes the time
Being 4 hours, the addition of described compound enzymic preparation is 10ppm (weight ratio), then that mud is naturally heavy
Fall air-dries.Arranging comparative example, the enzyme bacterium complexing agent of comparative example is according to CN 102250768 simultaneously
Preparation method described in B embodiment 1 prepares.
Table 1 compound enzymic preparation is applied to the treatment effect of the recycling treatment of mud
Compared with comparative example,*P ＜ 0.05,**P ＜ 0.01
As can be seen from Table 1, described compound enzymic preparation stink after the recycling treatment of mud all removes,
To the internal analysis of mud sampling it can be seen that described compound enzymic preparation for COD, BOD, SS and
NH3-N all has good obvious processing effect to be better than the enzyme bacterium complexing agent described in comparative example, wherein with reality
Execute the compound enzymic preparation treatment effect prepared by example 3 optimal.
Claims (7)
1., for a compound enzymic preparation for reuse of dredging process, it uses following preparation technology to prepare:
1) fermentation medium preparation: prepare fermentation medium according to following formula: the component of fermentation medium is
Glucose 10g, K2HPO42g, KH2PO42g, MgSO4·7H2O 0.2g, CaCl20.1g, carbamide 0.5g,
Yeast extract 0.5g, Chinese medicine extraction extractum 10g, pH7.2-7.5,120 DEG C sterilizing 30min, wherein Chinese medicine extraction
The preparation method of extractum is broken into coarse powder for taking recipe quantity natural plants, according to 4～9 times of additions of coarse powder gross weight
Volumetric concentration is the ethanol solution of 40%～95%, reflux, extract, three times, and return time is 2～5h, filters,
Filtrate recycling ethanol, cold filtration, wash with water, the most i.e. obtain Chinese medical concrete, described natural plants by
Alfalfa, schefflera, Bulbus Lilii and Caulis Hederae Sinensis composition；
2) biofermentation: after fermentation medium sterilizing, accesses the Rhodococcus coprophilus seed fermentation liquid of 10%, control
Fermentation temperature processed is 30-32 DEG C, ventilation is 1.5-1.7m3Cultivate 40-44h under/h, now access 10%
Aspergillus oryzae seed fermentation liquid, control fermentation temperature be 26-29 DEG C, ventilation be 1.8-2.2m3Train under/h
Support 26-30h, obtain containing enzyme fermentation liquid；
3) enzyme preparation is extracted: to containing adding the Tris-HCl solution regulation of 5 times of volumes in enzyme fermentation liquid to fermentation
The pH of liquid is between 5.4-6.2, ultrasonic 3-5min at 4 DEG C, 4 DEG C of centrifuging and taking supernatant of refrigerated centrifuger, to
Wherein adding the pre-cold acetone of 4 times of volumes ,-20 DEG C stand frozen centrifugation after 16-18 hour, take precipitation and use
After washing with acetone twice, obtain compound enzymic preparation.
Compound enzymic preparation the most according to claim 1, it is characterised in that described natural plants by
The component composition of following weight portion: alfalfa 35-45 part, schefflera 23-27 part, Bulbus Lilii 31-39 part,
Caulis Hederae Sinensis 8-12 part.
Compound enzymic preparation the most according to claim 2, it is characterised in that described natural plants is by such as
The component composition of lower weight portion: alfalfa 40 parts, schefflera 25 parts, Bulbus Lilii 35 parts, Caulis Hederae Sinensis 10
Part.
Compound enzymic preparation the most according to claim 1, it is characterised in that described Rhodococcus coprophilus and
Aspergillus oryzae is in exponential phase before accessing fermentation liquid, and its colony density is no less than 2 × 109cfu/mL。
5. claim 1-4 arbitrary described compound enzymic preparation application in the recycling treatment of mud.
Application the most according to claim 5, it is characterised in that mud is carried out by application compound enzymic preparation
The method of recycling treatment specifically includes following steps: adds compound enzymic preparation in mud and carries out anaerobic digestion,
The temperature of control mud is between 26-28 DEG C, and the process time is 3-6 hour, adding of described compound enzymic preparation
Entering amount is 5-15ppm, then mud natural subsidence is air-dried.
Application the most according to claim 6, it is characterised in that the described process time is 4 hours,
The addition of described compound enzymic preparation is 10ppm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282019A (en) * | 2016-08-25 | 2017-01-04 | 董晓 | A kind of preparation method of environment-friendly type soaping agent |
| CN106587553A (en) * | 2016-12-06 | 2017-04-26 | 钦州市高新技术产业服务中心 | Complex enzyme preparation and preparation method thereof |
| CN113005062A (en) * | 2021-03-24 | 2021-06-22 | 中国科学院成都生物研究所 | Facultative ammonia oxidizing bacteria and application thereof |
| CN114057441A (en) * | 2021-12-21 | 2022-02-18 | 浙江理工大学 | Device and method for regenerating filling material by using solid waste |
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| JPH08309327A (en) * | 1995-05-22 | 1996-11-26 | Mitsui Kushikino Kozan Kk | Garbage treatment apparatus |
| CN101182093A (en) * | 2007-12-07 | 2008-05-21 | 陈五岭 | Microbe harmless treatment method for oil-gas field waste slurry |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106282019A (en) * | 2016-08-25 | 2017-01-04 | 董晓 | A kind of preparation method of environment-friendly type soaping agent |
| CN106587553A (en) * | 2016-12-06 | 2017-04-26 | 钦州市高新技术产业服务中心 | Complex enzyme preparation and preparation method thereof |
| CN113005062A (en) * | 2021-03-24 | 2021-06-22 | 中国科学院成都生物研究所 | Facultative ammonia oxidizing bacteria and application thereof |
| CN114057441A (en) * | 2021-12-21 | 2022-02-18 | 浙江理工大学 | Device and method for regenerating filling material by using solid waste |
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