CN105884922A - Hot-dipping extraction method of crude plant polysaccharides - Google Patents
Hot-dipping extraction method of crude plant polysaccharides Download PDFInfo
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- CN105884922A CN105884922A CN201410741496.8A CN201410741496A CN105884922A CN 105884922 A CN105884922 A CN 105884922A CN 201410741496 A CN201410741496 A CN 201410741496A CN 105884922 A CN105884922 A CN 105884922A
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Abstract
The invention discloses a hot-dipping extraction method of crude plant polysaccharides. With a water system method, a plant sample for polysaccharide extraction is treated by five major steps including breaking and pulping, bath heating, centrifugation, washing and drying to finish crude extraction of plant polysaccharides; the extracted substances have a wide range; the method is suitable for extracting various plant polysaccharides and is easy to operate; the materials are easily available, the required conditions are simple, and the method is applicable to the extraction of free-state polysaccharides; and moreover, with few interfering substances, the method is safe to use in production, perfectly meets the practical requirements of domestic related industries and is suitable for large-scale promotion.
Description
Technical field
The present invention relates to field of biological detection, particularly relate to a kind of hot dipping and extract vegetalitas crude polysaccharides method.
Background technology
Vegetable polysaccharides, also known as plant polysaccharide, is the degree of polymerization that produces of the plant metabolism polysaccharide more than 10.The compound being made up of with α-or β-glycosidic bond many identical or different monosaccharide, is prevalent in natural plant body, including starch, cellulose, polysaccharide, pectin etc..Due to the wide material sources of vegetable polysaccharides, molecular composition and the molecular weight of vegetable polysaccharides the most of the same race are different.Some vegetable polysaccharides such as starch, cellulose, pectin, become the important component part in people's daily life already.Vegetable polysaccharides is extremely wide in distributed in nature, the most critically important, participates in the immunomodulating of organism, participates in the various activities of life cells.Has plenty of the constituent constituting plant cell wall, such as Peptidoglycan and cellulose；Has plenty of the nutrient as plant storage, such as glycogen and starch, vegetable polysaccharides such as lycium barbarum polysaccharide, lentinan, Auricularia polycose, laminarin, pine pollen polysaccharide, jujube polysaccharide, Polysaccharides in Bamboo Leaves, Herb Gynostemmae Pentaphylli polysaccharide, Cordyceps polysaccharide, Semen sojae atricolor crude polysaccharides, fig polysaccharide, Hericium Erinaceus Polysaccharide, A.chinensis Planch. polysaccharide, Rhizoma Atractylodis polysaccharide, sposknikovan, Dihuang polysaccharide, lycium barbarum polysaccharide, spirulina polysaccharide, eucommia bark polycose, the majorities such as Fructus Ligustri Lucidi polysaccharide are proteoglycans, there is two-ways regulation Human Physiology rhythm, blood sugar lowering, blood fat reducing, anti-inflammatory, antioxidation, defying age, radioprotective, anti-bacteria and anti-virus, the liver protecting, the functions such as antitumor.
In recent years, microwave auxiliary extraction technology is widely used in terms of polysaccharide extracting process.Patent of invention CN201310028640.9 discloses the extraction process of a kind of high efficiency extraction sea grass polysaccharide, in extracting in water step, at least two method in microwave method, supercritical ultrasonics technology, enzyme process is used to work in coordination with the extraction carrying out sea grass polysaccharide, in described supercritical ultrasonics technology, ultrasonic frequency is 3800-7500MHz, extraction time 1-3min, pH value is 5.0-5.5；In described microwave method, microwave frequency is 55-75kHz, extraction time 1-3min, and pH value is 5.0-5.5；In described enzyme process, enzyme addition is 0.8-2%, and pH value is 5.0-6.0, processes time 1.5-2h, reaction temperature 25-37 DEG C；In solid-liquid separation step, use the screen cloth of 150-250 mesh that the liquid after extracting is removed slag.This invention can be in the short period of time, it is achieved sea grass polysaccharide high efficiency extraction.But, the method there is also some defects, and its extraction time is unsuitable long, and power is unsuitable too high, and moisture otherwise can be caused vaporized many, and polysaccharide dissolution is obstructed, and causes polysaccharide yield to decline；Meanwhile, this method also reduces the activation energy of some reaction, makes between polysaccharide molecule, forms new active force between polysaccharide molecule and other molecule, increases the collision opportunity between molecule, stops the dissolution of polysaccharide molecule；It addition, equipment and instrument is required higher by the method, be not suitable for large-scale popularization.
Summary of the invention
For overcoming the deficiency in prior art, it is an object of the present invention to provide a kind of hot dipping and extract vegetalitas crude polysaccharides method.
For realizing the purpose of the present invention, technical scheme is as follows:
Vegetalitas crude polysaccharides method is extracted in a kind of hot dipping, and its method step is as follows:
(1) accurately weighing plant sample, the feed liquid ratio according to 1: 1 adds the ultra-pure water of equal quality, pulls an oar, be transferred in 500mL beaker, be placed in 80 degrees Celsius of thermostat water baths in refiner, extracts 3h；
(2) serosity after extracting is put in centrifuge and is centrifuged, and with the centrifugation 10min of 8000r/min, pours out supernatant；
(3) according to the volume of supernatant, according to the volume ratio of 1: 4, the dehydrated alcohol adding its tetraploid in supernatant long-pending carries out precipitate polysaccharides；
(4) this mixed liquor being placed in refrigerated overnight in 4 degrees Celsius of refrigerators, the speed then at 8000r/min is centrifuged 10min process to it afterwards, obtains pale yellow precipitate；
(5) repeatedly wash this precipitation in multiple times on a small quantity with ethanol solution, with the impurity in scavenger precipitation, until last gained supernatant, re-use till not developing the color when sulfuric acid-phynol method is tested；
(6) it is dried in the crude polysaccharides precipitation of last gained being placed in drying baker, obtains vegetalitas crude polysaccharides.
Beneficial effect: the present invention uses aqueous systems method, by the heat that carries out the plant sample of polysaccharide to be extracted crushing and pull an oar, bathes, it is centrifuged, washs, is dried five big steps and completes slightly extracting of plant polysaccharides, the species range extracted is wide, be suitable to the extraction of various vegetable polysaccharides and easy and simple to handle, material is easy to get, required condition is simple, it is applicable to the extraction of free state polysaccharide, and interfering material is few, safety is used on producing, meet very much the actual requirement of domestic relevant industries, suitable for large-scale promotion.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and be easy to understand with effect, below in conjunction with detailed description of the invention, the present invention is expanded on further.
Embodiment:
Vegetalitas crude polysaccharides method is extracted in a kind of hot dipping, and its method step is as follows:
(1) accurately weighing Arillus Longan sample, the feed liquid ratio according to 1: 1 adds the ultra-pure water of equal quality, pulls an oar, be transferred in 500mL beaker, be placed in 80 degrees Celsius of thermostat water baths in refiner, extracts 3h；
(2) serosity after extracting is put in centrifuge and is centrifuged, and with the centrifugation 10min of 8000r/min, pours out supernatant；
(3) according to the volume of supernatant, according to the volume ratio of 1: 4, the dehydrated alcohol adding its tetraploid in supernatant long-pending carries out precipitate polysaccharides；
(4) this mixed liquor being placed in refrigerated overnight in 4 degrees Celsius of refrigerators, the speed then at 8000r/min is centrifuged 10min process to it afterwards, obtains pale yellow precipitate；
(5) repeatedly wash this precipitation in multiple times on a small quantity with ethanol solution, with the impurity in scavenger precipitation, until last gained supernatant, re-use till not developing the color when sulfuric acid-phynol method is tested；
(6) it is dried in the crude polysaccharides precipitation of last gained being placed in drying baker, obtains Arillus Longan crude polysaccharides, use phenol one sulfuric acid process that the Arillus Longan crude polysaccharides content of gained is measured；
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every equivalent structure utilizing description of the invention content to be made or equivalence flow process conversion; or directly or indirectly it is used in other relevant technical fields, the most in like manner it is included in the scope of patent protection of the present invention.
Claims (2)
1. vegetalitas crude polysaccharides method is extracted in a hot dipping, it is characterised in that its method step is as follows:
(1) accurately weighing plant sample, the feed liquid ratio according to 1: 1 adds the ultra-pure water of equal quality,
Pull an oar in refiner, be transferred in 500mL beaker, be placed in 80 degrees Celsius of thermostat water baths, extract 3h；
(2) serosity after extracting is put in centrifuge and is centrifuged, with the centrifugation of 8000r/min
10min, pours out supernatant；
(3) according to the volume of supernatant, according to the volume ratio of 1: 4, in supernatant, its four times are added
The dehydrated alcohol of volume carries out precipitate polysaccharides；
(4) this mixed liquor is placed in refrigerated overnight in 4 degrees Celsius of refrigerators, afterwards then at 8000r/min's
Speed is centrifuged 10min process to it, is precipitated；
(5) repeatedly this precipitation is washed in multiple times on a small quantity with ethanol solution, with the impurity in scavenger precipitation,
Until last gained supernatant, re-use till not developing the color when sulphuric acid one phynol method is tested；
(6) it is dried in the crude polysaccharides precipitation of last gained being placed in drying baker, obtains vegetalitas crude polysaccharides.
Vegetalitas crude polysaccharides method is extracted in hot dipping the most according to claim 1, it is characterised in that described
In step (4), described after centrifugal obtained by be precipitated as pale yellow precipitate.
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| CN201410741496.8A CN105884922A (en) | 2014-12-09 | 2014-12-09 | Hot-dipping extraction method of crude plant polysaccharides |
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| CN201410741496.8A CN105884922A (en) | 2014-12-09 | 2014-12-09 | Hot-dipping extraction method of crude plant polysaccharides |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2021512997A (en) * | 2018-02-05 | 2021-05-20 | シャンハイ、グリーン、バレー、ファーマスーティカル、カンパニー、リミテッドＳｈａｎｇｈａｉ Ｇｒｅｅｎ Ｖａｌｌｅｙ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｃｏ．， Ｌｔｄ． | Separated windproof polysaccharides and their uses |
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2014
- 2014-12-09 CN CN201410741496.8A patent/CN105884922A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2021512997A (en) * | 2018-02-05 | 2021-05-20 | シャンハイ、グリーン、バレー、ファーマスーティカル、カンパニー、リミテッドＳｈａｎｇｈａｉ Ｇｒｅｅｎ Ｖａｌｌｅｙ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｃｏ．， Ｌｔｄ． | Separated windproof polysaccharides and their uses |
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Application publication date: 20160824 |