CN105866200A - Apparatus and method for evaluating treatment effect of etoposide - Google Patents
Apparatus and method for evaluating treatment effect of etoposide Download PDFInfo
- Publication number
- CN105866200A CN105866200A CN201610161131.7A CN201610161131A CN105866200A CN 105866200 A CN105866200 A CN 105866200A CN 201610161131 A CN201610161131 A CN 201610161131A CN 105866200 A CN105866200 A CN 105866200A
- Authority
- CN
- China
- Prior art keywords
- computer
- solution bottle
- signal
- etoposide
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses an apparatus and method for evaluating the treatment effect of etoposide. According to the invention, the peak value of the signal-to-noise ratio of etoposide with unknown drug effect is compared with the peak value of the signal-to-noise ratio of a frequently used cancer treatment drug, i.e., cisplatin, and the drug effect and dosage of etoposide are obtained in comparison with the known drug effect and dosage of cisplatin. The apparatus and method provided by the invention have the characteristics of high detection efficiency, good accuracy and capacity of providing reliable bases for safe usage of etoposide so as to ensure safety of patients.
Description
Technical field
The present invention relates to medicine effect detection technique field, especially relate to a kind of can accurately detect drug effect for depending on
The device and method of torr pool glycosides treatment effectiveness evaluation.
Background technology
The dosage of cancer therapy drug accurately when dose deficiency, must can not reach therapeutic effect.When dose is beyond safety
Dosage, then can cause the gastrointestinal reaction such as loss of appetite, nausea,vomiting,diarrhea.The most serious common toxic reaction is kidney poison
Property reaction, repeated drug taking can aggravate nephrotoxicity reaction, major determinant kidney proximal tubule, make Vacuole formation, exuviation, tube chamber
, there is hyaline cast in expansion, and in blood, uric acid is too much, can cause the symptoms such as hematuria time serious.
When heavy dose and repeatedly medication, it may appear that neurotoxicity, ear Ke hair cell for mouthpart can be damaged, cause height
Frequency loses to be listened.
The drug effect of PTS does not has clinical data to be referred to, and when the initial stage uses, fully rely on doctor gropes judgement,
If during dose deficiency, not reaching therapeutic effect；If dose can cause serious toxic reaction beyond safe dose, can give and suffer from
Person's health brings very major injury.
Summary of the invention
The goal of the invention of the present invention is to overcome the deficiency that the drug effect of PTS of the prior art is not clear, it is provided that
A kind of device and method for etoposide treatment effectiveness evaluation that can accurately detect drug effect.
To achieve these goals, the present invention is by the following technical solutions:
A kind of device for etoposide treatment effectiveness evaluation, including operating platform, the electrification being located on operating platform
Learn work station, 2 flow cells, fill cleaning solution the first solution bottle, fill etoposide solution the second solution bottle and
Brace；Support arm is provided with 2 hot-air blowers, is equipped with substrate, each substrate is equipped with electrode chip in each flow cell；The
Being equipped with catheter on the cover plate of one solution bottle and the second solution bottle, the catheter of the second solution bottle is provided with the first micro pump,
The catheter of the first solution bottle is provided with the second micro pump, the first micro pump and the second micro pump all by 2 drain pipes and two
Individual flow cell connects；Each electrode chip all include being connected with electrochemical workstation to electrode, reference electrode and working electrode,
Cultivating on working electrode and have several cancerous cell, the liquid outlet of each drain pipe is respectively positioned on above the outside of corresponding working electrode,
Each flow cell bottom is equipped with drain pipe；Electrochemical workstation, the first micro pump, the second micro pump and 2 hot-air blowers all with
Computer (14) electrically connects.
When etoposide solution drips on cancerous cell, cancerous cell can react, the electricity that electrochemical workstation detects
Current density signal can occur respective change, and detection signal Spect (t) of detected new drug is inputted and is pre-stored in calculating by the present invention
In second order stochastic resonance system in machine, computer draws the signal to noise ratio spectrogram of excitation noise signal, selects in signal to noise ratio spectrogram
Take the signal-to-noise ratio peak of the characteristic peak near initial point, and by the coordinate (M of signal-to-noise ratio peak1, M2) store in computer, calculate
In machine, storage has the characteristic peaks coordinate (W of cisplatin1, W2)；
When | M2| ＞ | W2| time, Computer display etoposide is better than cisplatin to the action effect of cancerous cell, etoposide
Dosage needs to reduce than cisplatin；
When | M2| ＜ | W2| time, Computer display etoposide is poorer than cisplatin to the action effect of cancerous cell, etoposide
Dosage needs to increase than cisplatin.
The present invention is by the noise of the signal-to-noise ratio peak of the etoposide of unknown drug effect with conventional cancer treatment drugs cisplatin
Compare than peak value, under the reference of the cisplatin of the clearest and the most definite drug effect and dose, it is thus achieved that the drug effect of etoposide and dose, for relying on
Pool glycosides safe handling provides reliable basis, it is ensured that the safety of patient.
As preferably, 2 vertical slots on operating platform, each vertical slot is equipped with top board and the cylinder being connected with top board, gas
The expansion link of cylinder is connected with top board lower surface, and two cylinders all electrically connect with computer；First solution bottle and the second solution bottle divide
It is not positioned on two top boards.
Two cylinders are used for driving top board and solution bottle lifting moving thereon, thus regulate the height of two micro pumps,
Reach to regulate the purpose of liquid droping speed.
As preferably, the first solution bottle and the second solution bottle being equipped with agitator, two agitators are all dynamo-electric with calculating
Connect；Hot-air blower is provided with temperature sensor, and temperature sensor electrically connects with computer；In first solution bottle and the second solution bottle
Being equipped with liquid level sensor, 2 liquid level sensors all electrically connect with computer.
As preferably, flow cell includes the housing of upper end open, the cover plate being located on housing and the sealing being located on cover plate
Circle, sealing ring lower surface is connected with the close base plate seals to electrode, working electrode and be provided with logical to the substrate between electrode
Hole, support arm is L-shaped.
A kind of method of device for etoposide treatment effectiveness evaluation, comprises the steps:
(5-1) computer controls hot-air blower electrified regulation, and the first micro pump drips cleaning solution, and cleaning solution flows through drain
Being heated during pipe, the first micro pump dropping liquid is after 8 to 20 minutes, and computer controls the first micro pump and stops dropping liquid；
(5-2) computer controls to stop stirring after the agitator on the second solution bottle works 8 to 16 minutes, and computer controls
Second micro pump drips etoposide solution, and after 8 to 10 minutes, 2 electric currents of computer acquisition electrochemical workstation detection are close
Degree signal S1(t), S2T (), computer calculates and obtains S1(t), S2T the average signal of (), is defined as electric current by average signal close
Degree signal S (t)；
Computer chooses the sampled value that several time intervals are Δ t, each sampled value in current density signal S (t)
It is arranged to make up detecting signal ES (t) according to time order and function order；
(5-3) for each sampled value ES (t outside in ES (t) first sampled value and last sampled value1), profit
Use formulaCalculate steady coefficient ratio；
Computer is previously provided with the weight threshold 0.5,1 and 1.5 increased successively；
Sampled value in the range of [1-A1,1+A1] is positioned at for ratio, sampled value is modified to B1ES (t1), B1 is little
In the real number of 0.4；
Sampled value in the range of (1-A2,1-A1) or (1+A1,1+A2) is positioned at for ratio, sampled value is modified to
B2ES(t1), B1 ＜ B2 ＜ 0.6
Sampled value in the range of [0.5,1-A2] or [1+A2,1.5] is positioned at for ratio, sampled value is modified to B3ES
(t1), B1 ＜ B2 ＜ B3≤0.8；
(5-4) replace the corresponding sampled value in ES (t) by each sampled value corrected, obtain the detection letter through revising
Number Spect (t),
In the second order stochastic resonance system be pre-stored in Spect (t) input in computer, computer draws excitation noise letter
Number signal to noise ratio spectrogram, signal to noise ratio spectrogram is chosen the signal-to-noise ratio peak of characteristic peak near initial point, and by signal-to-noise ratio peak
Coordinate (M1, M2) store in computer, in computer, storage has the characteristic peaks coordinate (W of cisplatin1, W2)；
(5-5) as | M2| ＞ | W2| time, Computer display etoposide is better than cisplatin to the action effect of cancerous cell, relies on
The dosage of pool glycosides needs to reduce than cisplatin；
When | M2| ＜ | W2| time, Computer display etoposide is poorer than cisplatin to the action effect of cancerous cell, etoposide
Dosage needs to increase than cisplatin.
As preferably, in the described second order stochastic resonance system that Spect (t) input is pre-stored in computer, computer
The signal to noise ratio spectrogram drawing excitation noise signal comprises the steps:
Spect (t) is inputted single order accidental resonance model by computer
In,
Wherein, (x, t) is potential function to V, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are the constant set, and ξ (t) is
Outer noise, D is outer noise intensity, and N (t) grasps noise in being,For periodic sinusoidal signal, A is signal amplitude,
F is signal frequency, and t is movement time,For phase place, ifcx2-dx4For
Demarcate component；
Computer calculate V (x, t) for first derivative and the second dervative of x, and makes equation be equal to 0, obtain two layers with
Machine resonance model:
Set noise intensity D=0,Spect (t)=0, N (t)=0；It is calculated the marginal value of A
For
The marginal value of A is substituted in one layer of accidental resonance model, and sets X0(t)=0, sn0=0, with quadravalence jade for asking rain Ge Kuta
One layer of accidental resonance model of Algorithm for Solving, obtains
And calculate
Wherein, xnT () is the n order derivative of x (t), snn-1It is the n-1 order derivative of S (t) value at t=0, snn+1It is S
The n+1 order derivative of (t) value at t=0, n=0,1 ..., N-1；Obtain x1(t), x2(t) ..., xn+1The value of (t)；
Computer is to x1(t), x2(t) ..., xn+1T () is integrated, obtain x (t), and obtain x (t) at one layer the most altogether
The second order stochastic resonance system of model and two layers of accidental resonance model composition of shaking produces the position x in accidental resonance momentmValue and xm
Corresponding resonance moment t1And and t1Corresponding noise D1, D1For a value in D；
Computer utilizes formulaCalculate the letter of second order stochastic resonance system output
Make an uproar ratio；Wherein, Δ U=a2/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal.
As preferably, the first solution bottle and the second solution bottle being equipped with agitator, two agitators are all dynamo-electric with calculating
Connect；
Also comprise the steps: before step (5-1)
Computer controls to stop stirring after the agitator on the first solution bottle works 12 to 20 minutes；
Also comprise the steps: before step (5-2)
Computer controls to stop stirring after the agitator on the second solution bottle works 12 to 20 minutes.
As preferably, hot-air blower is provided with temperature sensor, and temperature sensor electrically connects with computer；Also include walking as follows
Rapid:
The temperature of temperature sensor detection hot-air blower, is provided with the interval [T of standard temperature in computerL, TH], when the temperature of detection
Degree >=TH, computer controls electric heater and stops heating；Temperature≤T when detectionL, computer controls electric heater and begins to warm up.
As preferably, the first solution bottle and the second solution bottle are equipped with liquid level sensor, 2 liquid level sensors all with meter
Calculate mechatronics；It is characterized in that, 2 liquid level sensors detect the liquid level in the first solution bottle and the second solution bottle respectively, calculate
Being provided with standard value P in machine, the level value detected when the liquid level sensor of the first solution bottle is less than P, Computer display the first solution
The warning message that solution in Ping requires supplementation with；
The level value detected when the liquid level sensor of the second solution bottle less than P, molten in Computer display the second solution bottle
The warning message that liquid requires supplementation with.
As preferably, also comprise the steps: after step (5-5)
WhenTime, the dose that Computer display suggestion is increased or decreased is 5% to 15%；
WhenTime, the dose that Computer display suggestion is increased or decreased is 16% to 30%；
WhenTime, the dose that Computer display suggestion is increased or decreased is more than 30%.
Therefore, there is advantages that detection efficiency is high, accuracy is good, carry for etoposide safe handling
Supply reliable basis, it is ensured that the safety of patient.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation of the present invention；
Fig. 2 is a kind of structural representation of the groove of the present invention；
Fig. 3 is a kind of theory diagram of the present invention；
Fig. 4 is a kind of structural representation of the electrode chip of the present invention；
Fig. 5 is a kind of flow chart of embodiments of the invention；
Fig. 6 is a kind of signal to noise ratio spectrogram of the cisplatin of the present invention.
In figure: operating platform 1, electrochemical workstation 2, flow cell the 3, first solution bottle the 4, second solution bottle 5, support arm 6,
Hot-air blower 7, substrate 9, drain pipe 8, electrode chip the 10, first micro pump the 11, second micro pump 12, computer 14, agitator 15,
Temperature sensor 16, liquid level sensor 17, drain pipe 31, through hole 91, painting layer 92, vertical slot 101, top board 102, cylinder 103,
To electrode 1011, reference electrode 1012, working electrode 1013.
Detailed description of the invention
The present invention will be further described with detailed description of the invention below in conjunction with the accompanying drawings.
Embodiment as shown in Figure 1, Figure 3 is a kind of device for etoposide treatment effectiveness evaluation, flat including operation
Platform 1,2,2 flow cells 3 of the electrochemical workstation being located on operating platform, fills the first solution bottle 4 of cleaning solution, fills and depend on
Second solution bottle 5 of torr pool glycosides solution and support arm 6；Support arm is provided with 2 hot-air blowers 7, is equipped with base in each flow cell
Plate 9, each substrate is equipped with electrode chip 10；It is equipped with catheter on the cover plate of the first solution bottle and the second solution bottle, the
The catheter of two solution bottles is provided with the first micro pump 11, and the catheter of the first solution bottle is provided with the second micro pump 12, and first
Micro pump and the second micro pump are all connected with two flow cells by 2 drain pipes 8；It is right that the liquid outlet of each drain pipe is respectively positioned on
Answering above the outside of working electrode, each flow cell bottom is equipped with drain pipe 31；Each substrate all uses alloy platinum material to make.
As shown in Figure 4, each electrode chip all include being connected with electrochemical workstation to electrode 1011, reference electrode
1012 and working electrode 1013, working electrode is cultivated and has 23000 cancerous cell；
As it is shown on figure 3, electrochemical workstation, the first micro pump, the second micro pump and 2 hot-air blowers are all electric with computer 14
Connect.Being equipped with agitator 15 on first solution bottle and the second solution bottle, two agitators all electrically connect with computer；Hot-air blower
Being provided with temperature sensor 16, temperature sensor electrically connects with computer；First solution bottle and the second solution bottle are equipped with liquid
Level sensor 17,2 liquid level sensors all electrically connect with computer.
As in figure 2 it is shown, 2 vertical slots 101 on operating platform, each vertical slot is equipped with top board 102 and cylinder 103, cylinder
Expansion link be connected with top board lower surface, two cylinders all electrically connect with computer；First solution bottle and the second solution bottle are respectively
It is positioned on two top boards.
Flow cell includes the housing of upper end open, the cover plate being located on housing and the sealing ring being located on cover plate, sealing ring
Lower surface is connected with the close base plate seals to electrode.
As shown in Figure 4, working electrode and the substrate between electrode is provided with through hole 91, also include painting layer 92,
Brace is inverted L-shaped.
As it is shown in figure 5, a kind of method of device for etoposide treatment effectiveness evaluation, comprise the steps:
Step 100, preheats, cleans
Computer controls to stop stirring after the agitator on the first solution bottle works 18 minutes；Computer controls electric heater and leads to
Electrical heating, the temperature of temperature sensor detection hot-air blower, computer is provided with the interval [T of standard temperatureL, TH], when the temperature of detection
≥TH, computer controls electric heater and stops heating；Temperature≤T when detectionL, computer controls electric heater and begins to warm up TL=
36.5 DEG C, TH=39.6 DEG C；
First micro pump drips cleaning solution, and cleaning solution flows through during catheter heated, the first micro pump dropping liquid 20 points
Zhong Hou, computer controls the first micro pump and stops dropping liquid；Cleaning solution is phosphate buffer.
Step 200, detection
Computer controls to stop stirring after the agitator on the second solution bottle works 16 minutes, and computer controls the second trace
The pump etoposide solution that drips is after 10 minutes, 2 current density signal S of computer acquisition electrochemical workstation detection1(t), S2
T (), computer calculates and obtains S1(t), S2T the average signal of (), is defined as current density signal S (t) by average signal；
Computer controls the second micro pump and drips etoposide solution, and after the second micro pump dropping liquid 10 minutes, computer is adopted
Current density signal S (t) of collection electrochemical workstation detection, 400 times chosen in current density signal S (t) by computer
Being spaced apart the sampled value of Δ t, each sampled value is arranged to make up detecting signal ES (t) according to time order and function order；
Step 300, data correction
For each sampled value ES (t outside in ES (t) first sampled value and last sampled value1), utilize public affairs
FormulaCalculate steady coefficient ratio；
Computer is previously provided with the weight threshold 0.5,1 and 1.5 increased successively；
Sampled value in the range of [1-A1,1+A1] is positioned at for ratio, sampled value is modified to B1ES (t1), A1 is
0.16, B1 is 0.2；
Sampled value in the range of (1-A2,1-A1) or (1+A1,1+A2) is positioned at for ratio, sampled value is modified to
B2ES(t1), A2 is 0.32；
Sampled value in the range of [0.5,1-A2] or [1+A2,1.5] is positioned at for ratio, sampled value is modified to B3ES
(t1), B3 is 0.7.
Step 400, the signal-to-noise ratio peak at selected characteristic peak
Replace the corresponding sampled value in ES (t) by each sampled value corrected, obtain the detection signal through revising
Spect (t),
In described second order stochastic resonance system Spect (t) input being pre-stored in computer, computer draws excitation and makes an uproar
The signal to noise ratio spectrogram of acoustical signal comprises the steps:
Spect (t) is inputted single order accidental resonance model by computer
In,
Wherein, (x, t) is potential function to V, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are the constant set, and ξ (t) is
Outer noise, D is outer noise intensity, and N (t) grasps noise in being,For periodic sinusoidal signal, A is signal amplitude,
F is signal frequency, and t is movement time,For phase place, ifcx2-dx4For
Demarcate component；
Computer calculate V (x, t) for first derivative and the second dervative of x, and makes equation be equal to 0, obtain two layers with
Machine resonance model:
Set noise intensity D=0,Spect (t)=0, N (t)=0；It is calculated the marginal value of A
For
The marginal value of A is substituted in one layer of accidental resonance model, and sets X0(t)=0, sn0=0, with quadravalence jade for asking rain Ge Kuta
One layer of accidental resonance model of Algorithm for Solving, obtains
And calculate
Wherein, xnT () is the n order derivative of x (t), snn-1It is the n-1 order derivative of S (t) value at t=0, snn+1It is S
The n+1 order derivative of (t) value at t=0, n=0,1 ..., N-1；Obtain x1(t), x2(t) ..., xn+1The value of (t)；
Computer is to x1(t), x2(t) ..., xn+1T () is integrated, obtain x (t), and obtain x (t) at one layer the most altogether
The second order stochastic resonance system of model and two layers of accidental resonance model composition of shaking produces the position x in accidental resonance momentmValue and xm
Corresponding resonance moment t1And and t1Corresponding noise D1, D1For a value in D；
Computer utilizes formulaCalculate the letter of second order stochastic resonance system output
Make an uproar ratio；Wherein, Δ U=a2/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal；
The signal-to-noise ratio peak of the characteristic peak near initial point is chosen in signal to noise ratio spectrogram, and by the coordinate of signal-to-noise ratio peak
(M1, M2) store in computer, in computer, storage has the characteristic peaks coordinate (W of cisplatin1, W2)；The characteristic peaks of cisplatin is sat
Mark (W1, W2) it is that the method using step 100 to 400 obtains equally, it is contained in the cisplatin solution in 2 the second solution bottles respectively
Identical with etoposide concentration.
Step 500, makes action effect and dose judges
When | M2| ＞ | W2| time, Computer display etoposide is better than cisplatin to the action effect of cancerous cell, etoposide
Dosage needs to reduce than cisplatin；
When | M2| ＜ | W2| time, Computer display etoposide is poorer than cisplatin to the action effect of cancerous cell, etoposide
Dosage needs to increase than cisplatin；
WhenTime, the dose that Computer display suggestion is increased or decreased is 5% to 15%；
WhenTime, the dose that Computer display suggestion is increased or decreased is 16% to 30%；
WhenTime, the dose that Computer display suggestion is increased or decreased is more than 30%.
As shown in Figure 6, the signal-to-noise ratio peak of the characteristic peak of cisplatin is (20 ,-34dB), the feature of the etoposide of the present invention
The signal-to-noise ratio peak at peak is (8 ,-45dB)；Then Computer display harringtonine is thin to cancer
The chemotherapy effect of born of the same parents is better than cisplatin, and the dosage of etoposide needs the information reduced than cisplatin, can advise reducing 30%.
Also comprise the steps:
2 liquid level sensors detect the liquid level in the first solution bottle and the second solution bottle respectively, are provided with standard in computer
Value P, the level value detected when the liquid level sensor of the first solution bottle needs less than P, the solution in Computer display the first solution bottle
Warning message to be supplemented；
The level value detected when the liquid level sensor of the second solution bottle less than P, molten in Computer display the second solution bottle
The warning message that liquid requires supplementation with.
Should be understood that the present embodiment is merely to illustrate the present invention rather than limits the scope of the present invention.In addition, it is to be understood that
After having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, these etc.
Valency form falls within the application appended claims limited range equally.
Claims (10)
1. for a device for etoposide treatment effectiveness evaluation, it is characterized in that, including operating platform (1), be located at operation flat
Electrochemical workstation (2) on platform, 2 flow cells (3), fill first solution bottle (4) of cleaning solution, to fill etoposide molten
Second solution bottle (5) of liquid and support arm (6)；Support arm is provided with 2 hot-air blowers (7), is equipped with substrate in each flow cell
(9), each substrate is equipped with electrode chip (10)；It is equipped with catheter on the cover plate of the first solution bottle and the second solution bottle,
The catheter of the second solution bottle is provided with the first micro pump (11), and the catheter of the first solution bottle is provided with the second micro pump
(12), the first micro pump and the second micro pump are all connected with two flow cells by 2 drain pipes (8)；Each electrode chip is equal
Including be connected with electrochemical workstation to electrode (1011), reference electrode (1012) and working electrode (1013), working electrode
Upper cultivation has several cancerous cell, and the liquid outlet of each drain pipe is respectively positioned on above the outside of corresponding working electrode, each circulation
Bottom, pond is equipped with drain pipe (31)；Electrochemical workstation, the first micro pump, the second micro pump and 2 hot-air blowers all with calculate
Machine (14) electrically connects.
Device for etoposide treatment effectiveness evaluation the most according to claim 1, is characterized in that, on operating platform 2
Individual vertical slot (101), is equipped with top board (102) and the cylinder (103) being connected with top board in each vertical slot；First solution bottle and second
Solution bottle lays respectively on two top boards.
Device for etoposide treatment effectiveness evaluation the most according to claim 1, is characterized in that, the first solution bottle and
Being equipped with agitator (15) on second solution bottle, two agitators all electrically connect with computer；Hot-air blower is provided with temperature sensing
Device (16), temperature sensor electrically connects with computer；First solution bottle and the second solution bottle are equipped with liquid level sensor (17),
2 liquid level sensors all electrically connect with computer.
4., according to the device for etoposide treatment effectiveness evaluation described in claim 1 or 2 or 3, it is characterized in that, flow cell
Including the housing of upper end open, the cover plate being located on housing and the sealing ring being located on cover plate, sealing ring lower surface is right with close
The base plate seals of electrode connects, working electrode and the substrate between electrode is provided with through hole (91), and support arm is L-shaped.
5. a method based on the device for etoposide treatment effectiveness evaluation described in claim 1, is characterized in that, bag
Include following steps:
(5-1) computer controls hot-air blower electrified regulation, and the first micro pump drips cleaning solution, when cleaning solution flows through catheter
Heated, the first micro pump dropping liquid is after 8 to 20 minutes, and computer controls the first micro pump and stops dropping liquid；
(5-2) computer controls to stop stirring after the agitator on the second solution bottle works 8 to 16 minutes, and computer controls second
Micro pump drips etoposide solution, after 8 to 10 minutes, and 2 electric current densities letters of computer acquisition electrochemical workstation detection
Number S1(t), S2T (), computer calculates and obtains S1(t), S2T the average signal of (), is defined as electric current density letter by average signal
Number S (t)；
The sampled value that several time intervals are Δ t chosen in current density signal S (t) by computer, each sampled value according to
Time order and function order is arranged to make up detecting signal ES (t)；
(5-3) for each sampled value ES (t outside in ES (t) first sampled value and last sampled value1), utilize public affairs
FormulaCalculate steady coefficient ratio；
Computer is previously provided with the weight threshold 0.5,1 and 1.5 increased successively；
Sampled value in the range of [1-A1,1+A1] is positioned at for ratio, sampled value is modified to B1ES (t1), B1 is less than 0.4
Real number；
Sampled value in the range of (1-A2,1-A1) or (1+A1,1+A2) is positioned at for ratio, sampled value is modified to B2ES
(t1), B1 ＜ B2 ＜ 0.6
Sampled value in the range of [0.5,1-A2] or [1+A2,1.5] is positioned at for ratio, sampled value is modified to B3ES (t1),
B1 ＜ B2 ＜ B3≤0.8；
(5-4) replace the corresponding sampled value in ES (t) by each sampled value corrected, obtain the detection signal through revising
Spect (t),
In the second order stochastic resonance system be pre-stored in Spect (t) input in computer, computer draws excitation noise signal
Signal to noise ratio spectrogram, chooses the signal-to-noise ratio peak of the characteristic peak near initial point in signal to noise ratio spectrogram, and by the seat of signal-to-noise ratio peak
Mark (M1, M2) store in computer, in computer, storage has the characteristic peaks coordinate (W of cisplatin1, W2)；
(5-5) as | M2| ＞ | W2| time, Computer display etoposide is better than cisplatin to the action effect of cancerous cell, etoposide
Dosage need than cisplatin reduce；
When | M2| ＜ | W2| time, Computer display etoposide is poorer than cisplatin to the action effect of cancerous cell, the medication of etoposide
Amount needs to increase than cisplatin.
The method of the device for etoposide treatment effectiveness evaluation the most according to claim 5, is characterized in that,
In described second order stochastic resonance system Spect (t) input being pre-stored in computer, computer draws excitation noise letter
Number signal to noise ratio spectrogram comprise the steps:
Spect (t) is inputted single order accidental resonance model by computer
In, wherein, (x, t) is potential function to V, and x (t) is Brownian movement Particles Moving lopcus function, and a, b, c, d are the constant set, ξ
T () is outer noise, D is outer noise intensity, and N (t) grasps noise in being,For periodic sinusoidal signal, A is letter
Number amplitude, f is signal frequency, and t is movement time,For phase place, if
cx2-dx4For demarcating component；
Computer calculates V, and (x, t) for first derivative and the second dervative of x, and makes equation be equal to 0, obtains two layers the most altogether
Shake model:
Set noise intensity D=0,Spect (t)=0, N (t)=0；The marginal value being calculated A is
The marginal value of A is substituted in one layer of accidental resonance model, and sets X0(t)=0, sn0=0, with quadravalence jade for asking rain Ge Kuta algorithm
Solve one layer of accidental resonance model, obtain
And calculate
Wherein, xnT () is the n order derivative of x (t), snn-1It is the n-1 order derivative of S (t) value at t=0, snn+1It is the n+ of S (t)
1 order derivative value at t=0, n=0,1 ..., N-1；Obtain x1(t), x2(t) ..., xn+1The value of (t)；
Computer is to x1(t), x2(t) ..., xn+1T () is integrated, obtain x (t), and obtain x (t) at one layer of accidental resonance mould
The second order stochastic resonance system of type and two layers of accidental resonance model composition produces the position x in accidental resonance momentmValue and xmRelatively
The resonance moment t answered1And and t1Corresponding noise D1, D1For a value in D；
Computer utilizes formulaCalculate the signal to noise ratio of second order stochastic resonance system output；
Wherein, Δ U=a2/4b；Computer draws the signal to noise ratio spectrogram of excitation noise signal.
The method of the device for etoposide treatment effectiveness evaluation the most according to claim 5, the first solution bottle and
Being equipped with agitator on two solution bottles, two agitators all electrically connect with computer；It is characterized in that,
Also comprise the steps: before step (5-1)
Computer controls to stop stirring after the agitator on the first solution bottle works 12 to 20 minutes；
Also comprise the steps: before step (5-2)
Computer controls to stop stirring after the agitator on the second solution bottle works 12 to 20 minutes.
The method of the device for etoposide treatment effectiveness evaluation the most according to claim 5, hot-air blower is provided with temperature
Degree sensor, temperature sensor electrically connects with computer；It is characterized in that, also comprise the steps:
The temperature of temperature sensor detection hot-air blower, is provided with the interval [T of standard temperature in computerL, TH], when detection temperature >=
TH, computer controls hot-air blower and stops heating；Temperature≤T when detectionL, computer controls hot-air blower and begins to warm up.
9., according to the method for the device for etoposide treatment effectiveness evaluation described in claim 5 or 6 or 7 or 8, first is molten
Being equipped with liquid level sensor in liquid bottle and the second solution bottle, 2 liquid level sensors all electrically connect with computer；It is characterized in that, 2
Liquid level sensor detects the liquid level in the first solution bottle and the second solution bottle respectively, is provided with standard value P in computer, when first is molten
The level value of the liquid level sensor detection of liquid bottle is less than P, the warning that the solution in Computer display the first solution bottle requires supplementation with
Information；
The level value detected when the liquid level sensor of the second solution bottle needs less than P, the solution in Computer display the second solution bottle
Warning message to be supplemented.
10., according to the method for the device for etoposide treatment effectiveness evaluation described in claim 5 or 6 or 7 or 8, it is special
Levy and be, also comprise the steps: after step (5-5)
WhenTime, the dose that Computer display suggestion is increased or decreased is 5% to 15%；
WhenTime, the dose that Computer display suggestion is increased or decreased is 16% to 30%；
WhenTime, the dose that Computer display suggestion is increased or decreased is more than 30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610161131.7A CN105866200B (en) | 2016-03-21 | 2016-03-21 | A kind of device and method for Etoposide treatment effectiveness evaluation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610161131.7A CN105866200B (en) | 2016-03-21 | 2016-03-21 | A kind of device and method for Etoposide treatment effectiveness evaluation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105866200A true CN105866200A (en) | 2016-08-17 |
| CN105866200B CN105866200B (en) | 2018-04-10 |
Family
ID=56624791
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610161131.7A Active CN105866200B (en) | 2016-03-21 | 2016-03-21 | A kind of device and method for Etoposide treatment effectiveness evaluation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105866200B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113901869A (en) * | 2021-09-02 | 2022-01-07 | 电子科技大学 | Non-contact liquid level detection method based on Spiking neural network |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105823816A (en) * | 2016-01-04 | 2016-08-03 | 杭州电子科技大学 | Detection apparatus and detection method for drug effect of anti-cancer drug etoposide |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242181A (en) * | 2011-04-28 | 2011-11-16 | 中国烟草总公司郑州烟草研究院 | Flue gas condensate cytotoxicity determination method based on cell electronic sensor |
| CN102796693A (en) * | 2012-08-24 | 2012-11-28 | 中国科学院生态环境研究中心 | Microbial cell sensor for detecting arsenic bioavailability degree |
| CN102818826A (en) * | 2012-07-21 | 2012-12-12 | 上海师范大学 | Electrochemical cell-based biosensor based on nanometer Ag@BSA biomimetic interface and preparation method thereof |
| CN102911906A (en) * | 2012-11-19 | 2013-02-06 | 中国科学院生态环境研究中心 | Microbial cell sensor for detecting bioavailability of cadmium |
| CN103387973A (en) * | 2012-05-09 | 2013-11-13 | 中国科学院生态环境研究中心 | Immobilization method for improving detection effect of benzene series cell sensors |
| CN104250634A (en) * | 2014-02-28 | 2014-12-31 | 曾杰 | Logic cell model design and synthesis method for biosensor |
| CN105181561A (en) * | 2015-09-02 | 2015-12-23 | 江苏大学 | Hemocyte analysis sensor |
-
2016
- 2016-03-21 CN CN201610161131.7A patent/CN105866200B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242181A (en) * | 2011-04-28 | 2011-11-16 | 中国烟草总公司郑州烟草研究院 | Flue gas condensate cytotoxicity determination method based on cell electronic sensor |
| CN103387973A (en) * | 2012-05-09 | 2013-11-13 | 中国科学院生态环境研究中心 | Immobilization method for improving detection effect of benzene series cell sensors |
| CN102818826A (en) * | 2012-07-21 | 2012-12-12 | 上海师范大学 | Electrochemical cell-based biosensor based on nanometer Ag@BSA biomimetic interface and preparation method thereof |
| CN102796693A (en) * | 2012-08-24 | 2012-11-28 | 中国科学院生态环境研究中心 | Microbial cell sensor for detecting arsenic bioavailability degree |
| CN102911906A (en) * | 2012-11-19 | 2013-02-06 | 中国科学院生态环境研究中心 | Microbial cell sensor for detecting bioavailability of cadmium |
| CN104250634A (en) * | 2014-02-28 | 2014-12-31 | 曾杰 | Logic cell model design and synthesis method for biosensor |
| CN105181561A (en) * | 2015-09-02 | 2015-12-23 | 江苏大学 | Hemocyte analysis sensor |
Non-Patent Citations (1)
| Title |
|---|
| GUOHUA HUI等: "Tastant quantitative analysis from complex mixtures using taste cell-based sensor and double-layered cascaded series stochastic resonance", 《ELECTROCHIMICA ACTE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113901869A (en) * | 2021-09-02 | 2022-01-07 | 电子科技大学 | Non-contact liquid level detection method based on Spiking neural network |
| CN113901869B (en) * | 2021-09-02 | 2023-04-21 | 电子科技大学 | Non-contact liquid level detection method based on Spiking neural network |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105866200B (en) | 2018-04-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ES2543911T3 (en) | Capacity detection in electrochemical test with improved sampling time check | |
| ES2456899T3 (en) | Capacitance detection in electrochemical test | |
| JPS63181738A (en) | Apparatus for examination of fluid medium and operation thereof | |
| US20060107729A1 (en) | Systems and methods for calibrating osmolarity measuring devices | |
| CN105866200A (en) | Apparatus and method for evaluating treatment effect of etoposide | |
| EP1745271A2 (en) | Systems and methods for reducing the effect of corruptive signals during nanoliter osmometry | |
| CN105823807B (en) | Cancer therapy drug Hydroxycamptothecin Composition analyzed device and detection method | |
| CN106560030A (en) | Real-time control device for dissolved oxygen for pond and control method thereof | |
| CA3060910A1 (en) | Analyte measurement system and method | |
| CN105823808B (en) | Cancer therapy drug topotecan Composition analyzed device and detection method | |
| CN105823816A (en) | Detection apparatus and detection method for drug effect of anti-cancer drug etoposide | |
| CN105866217B (en) | Harringtonine treatment of cancer effect detection device and method | |
| CN105823809A (en) | Detection apparatus and detection method for chemotherapy effect of gemcitabine | |
| CN105806904A (en) | Device and method for detecting efficacy of anti-cancer drug, ifosfamide | |
| CN105823815B (en) | Anticancer drug docetaxel Composition analyzed device and detection method | |
| CN105866210A (en) | Detection apparatus and detection method for drug effect of anti-cancer drug vincristine | |
| CN105891283A (en) | Medicine effect quantification device and method for elemene | |
| CN105823810A (en) | Detection apparatus and detection method for chemotherapy effect of paclitaxel | |
| CN101975640A (en) | Device for detecting blocking pressure and pill quantity of infusion pump/injection pump | |
| CN212228135U (en) | Micro liquid flow measuring device based on conductivity measurement | |
| CN108106689A (en) | A kind of modeling method and device using pressure sensor on-line monitoring agricultural machinery medicine-chest residue drug storage amount | |
| CN204618247U (en) | Hemodialysis carbamide real-time monitoring sensing device | |
| Sheybani et al. | Design, fabrication, and characterization of an electrochemically-based dose tracking system for closed-loop drug delivery | |
| BLINKS | Method for study of contraction of isolated heart muscle under various physical conditions | |
| WO2005089210A2 (en) | Systems and methods for collecting and analysing tear film osmolarity data |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |