CN105861727B - 用于检测3种眼科感染螺旋体的lamp引物组合及应用 - Google Patents
用于检测3种眼科感染螺旋体的lamp引物组合及应用 Download PDFInfo
- Publication number
- CN105861727B CN105861727B CN201610403954.6A CN201610403954A CN105861727B CN 105861727 B CN105861727 B CN 105861727B CN 201610403954 A CN201610403954 A CN 201610403954A CN 105861727 B CN105861727 B CN 105861727B
- Authority
- CN
- China
- Prior art keywords
- primer
- sequence
- single strand
- strand dna
- sequence table
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000000203 mixture Substances 0.000 title abstract description 24
- 208000015181 infectious disease Diseases 0.000 title abstract description 18
- 208000016604 Lyme disease Diseases 0.000 claims abstract description 73
- 241000589968 Borrelia Species 0.000 claims abstract description 70
- 241000589884 Treponema pallidum Species 0.000 claims abstract description 69
- 241000589902 Leptospira Species 0.000 claims abstract description 66
- 238000000034 method Methods 0.000 claims abstract description 27
- 230000003321 amplification Effects 0.000 claims description 73
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 73
- 238000006243 chemical reaction Methods 0.000 claims description 26
- 238000002360 preparation method Methods 0.000 claims description 17
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 16
- 201000010099 disease Diseases 0.000 claims description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 79
- 239000000523 sample Substances 0.000 description 58
- 239000013612 plasmid Substances 0.000 description 23
- 239000012925 reference material Substances 0.000 description 23
- 238000012217 deletion Methods 0.000 description 18
- 230000037430 deletion Effects 0.000 description 18
- 239000002773 nucleotide Substances 0.000 description 18
- 125000003729 nucleotide group Chemical group 0.000 description 18
- 238000006467 substitution reaction Methods 0.000 description 18
- 238000001514 detection method Methods 0.000 description 15
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 12
- 208000006379 syphilis Diseases 0.000 description 12
- 210000001508 eye Anatomy 0.000 description 10
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 7
- 230000001717 pathogenic effect Effects 0.000 description 7
- 239000004471 Glycine Substances 0.000 description 6
- 239000012154 double-distilled water Substances 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 6
- 101000686153 Homo sapiens Ras-related GTP-binding protein A Proteins 0.000 description 4
- 102100022219 NF-kappa-B essential modulator Human genes 0.000 description 4
- 101710090077 NF-kappa-B essential modulator Proteins 0.000 description 4
- 102100025001 Ras-related GTP-binding protein A Human genes 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 208000035473 Communicable disease Diseases 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 241001215120 Leptospirales Species 0.000 description 3
- 102100031822 Optineurin Human genes 0.000 description 3
- 101710131459 Optineurin Proteins 0.000 description 3
- 206010046851 Uveitis Diseases 0.000 description 3
- 230000001154 acute effect Effects 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 208000030533 eye disease Diseases 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 238000007397 LAMP assay Methods 0.000 description 2
- 206010024238 Leptospirosis Diseases 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000004438 eyesight Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 206010000234 Abortion spontaneous Diseases 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 206010051625 Conjunctival hyperaemia Diseases 0.000 description 1
- 206010010741 Conjunctivitis Diseases 0.000 description 1
- 208000003164 Diplopia Diseases 0.000 description 1
- 206010015995 Eyelid ptosis Diseases 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 208000008771 Lymphadenopathy Diseases 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000034493 Mucous membrane disease Diseases 0.000 description 1
- 208000009525 Myocarditis Diseases 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 206010000594 acrodermatitis chronica atrophicans Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 210000001742 aqueous humor Anatomy 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 201000006901 congenital syphilis Diseases 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 208000022532 enlargement of lymph nodes Diseases 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 208000021760 high fever Diseases 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 206010023332 keratitis Diseases 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 208000015994 miscarriage Diseases 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 201000003004 ptosis Diseases 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000012113 quantitative test Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 210000003786 sclera Anatomy 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 208000000995 spontaneous abortion Diseases 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 210000001745 uvea Anatomy 0.000 description 1
- 230000003156 vasculitic effect Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610403954.6A CN105861727B (zh) | 2016-06-08 | 2016-06-08 | 用于检测3种眼科感染螺旋体的lamp引物组合及应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610403954.6A CN105861727B (zh) | 2016-06-08 | 2016-06-08 | 用于检测3种眼科感染螺旋体的lamp引物组合及应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105861727A CN105861727A (zh) | 2016-08-17 |
CN105861727B true CN105861727B (zh) | 2019-06-18 |
Family
ID=56676236
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610403954.6A Active CN105861727B (zh) | 2016-06-08 | 2016-06-08 | 用于检测3种眼科感染螺旋体的lamp引物组合及应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105861727B (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106754907A (zh) * | 2017-01-11 | 2017-05-31 | 博奥生物集团有限公司 | 用于检测产超广谱β‑内酰胺酶三种细菌耐药基因的LAMP引物组合及其应用 |
CN107385069A (zh) * | 2017-08-17 | 2017-11-24 | 北京出入境检验检疫局检验检疫技术中心 | 一种用于检测梅毒螺旋体的lamp引物组、试剂盒和方法 |
PL237232B1 (pl) * | 2017-10-20 | 2021-03-22 | Genomtec Spolka Akcyjna | Zestaw starterów do powielania sekwencji nukleotydowej Borrelia burgdorferi, sposób wykrywania Borrelia burgdorferi, sposób diagnostyki boreliozy i zestaw do diagnostyki boreliozy |
CN110129466A (zh) * | 2018-02-08 | 2019-08-16 | 北京智德医学检验所有限公司 | 用于检测眼内液中白色念珠菌的lamp引物组合及应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101225440A (zh) * | 2007-12-03 | 2008-07-23 | 浙江大学 | 一种钩端螺旋体的检测方法 |
CN101967513A (zh) * | 2009-12-10 | 2011-02-09 | 中国农业科学院兰州兽医研究所 | 区分莱姆病病原基因型的试剂及方法 |
-
2016
- 2016-06-08 CN CN201610403954.6A patent/CN105861727B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101225440A (zh) * | 2007-12-03 | 2008-07-23 | 浙江大学 | 一种钩端螺旋体的检测方法 |
CN101967513A (zh) * | 2009-12-10 | 2011-02-09 | 中国农业科学院兰州兽医研究所 | 区分莱姆病病原基因型的试剂及方法 |
Non-Patent Citations (1)
Title |
---|
Multiplex Loop-Mediated Isothermal Amplification Detection by Sequence-Based Barcodes Coupled with Nicking Endonuclease-Mediated Pyrosequencing;Chao Liang等;《Analytical Chemistry》;20121231;第84卷;3758-3763 * |
Also Published As
Publication number | Publication date |
---|---|
CN105861727A (zh) | 2016-08-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105861727B (zh) | 用于检测3种眼科感染螺旋体的lamp引物组合及应用 | |
Katzwinkel‐Wladarsch et al. | Direct amplification and species determination of microsporidian DNA from stool specimens | |
Lodh et al. | Point of care diagnosis of multiple schistosome parasites: Species-specific DNA detection in urine by loop-mediated isothermal amplification (LAMP) | |
King et al. | Development of a TaqMan® PCR assay with internal amplification control for the detection of African swine fever virus | |
CN104805218B (zh) | 基于lamp和分子信标检测hpv16和18的反应体系和方法 | |
Almasi et al. | Development of colorimetric loop-mediated isothermal amplification assay for rapid detection of the tomato yellow leaf curl virus | |
CN105624329B (zh) | 单纯疱疹病毒1型实时荧光核酸恒温扩增检测试剂盒 | |
CN108676910A (zh) | 一种层出镰刀菌的lamp检测引物及其应用 | |
WO2022257663A1 (zh) | 一种检测筛查新冠病毒n501y突变的方法及试剂盒 | |
Poole et al. | In silico identification of novel biomarkers and development of new rapid diagnostic tests for the filarial parasites Mansonella perstans and Mansonella ozzardi | |
CN103045761B (zh) | 用于检测感染性眼病致病微生物的试剂盒及检测方法 | |
da Fonseca et al. | Comparison of three human papillomavirus DNA detection methods: next generation sequencing, multiplex‐PCR and nested‐PCR followed by sanger based sequencing | |
Gomez et al. | Dual-target, real-time PCR for the diagnosis of intraocular Toxoplasma gondii infections | |
Kim et al. | Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea | |
CN107881261A (zh) | 检测猪圆环病毒3型的lamp引物组、试剂盒及应用 | |
Van Gelder | CME review: polymerase chain reaction diagnostics for posterior segment disease | |
CN107287351A (zh) | 环介导等温扩增结合侧向流动试纸条检测锦鲤疱疹病毒 | |
Nayak et al. | Molecular characterization of drug-resistant and drug-sensitive Aspergillus isolates causing infectious keratitis | |
Titilayo et al. | Genetic variations among three major ethnic groups in Nigeria using RAPD | |
CN108866164A (zh) | B族链球菌的检测方法及试剂盒 | |
CN108359743A (zh) | 一种用于鉴别fpv和cpv的hrm引物组,含有该引物组的试剂盒及其应用 | |
Sohrabi et al. | The prevalence of viral conjunctivitis in patients who referred to eye specialist hospitals in Tehran, Iran | |
CN108707688A (zh) | 检测植物病原性轮枝菌的特异引物对、探针和试剂盒及其应用 | |
Weitgasser et al. | Evaluation of polymerase chain reaction for the detection of adenoviruses in conjunctival swab specimens using degenerate primers in comparison with direct immunofluorescence | |
Wang | Rapid differentiation of Candida albicans from non‐C. albicans directly in a variety of clinical specimens using fluorescent in situ hybridisation |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20210519 Address after: 101300 second floor, building 37, lanxiyuan District 3, Renhe Town, Shunyi District, Beijing Patentee after: BEIJING ZHIDE MEDICAL LABORATORY Co.,Ltd. Address before: 8 worker's Stadium South Road, Chaoyang District, Beijing 100020 Patentee before: BEIJING CHAO-YANG HOSPITAL, CAPITAL MEDICAL University |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Lamp primer combination and application for detecting three kinds of ophthalmic infection spirochetes Effective date of registration: 20220114 Granted publication date: 20190618 Pledgee: Zhongguancun Beijing technology financing Company limited by guarantee Pledgor: BEIJING ZHIDE MEDICAL LABORATORY CO.,LTD. Registration number: Y2022990000023 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220610 Granted publication date: 20190618 Pledgee: Zhongguancun Beijing technology financing Company limited by guarantee Pledgor: BEIJING ZHIDE MEDICAL LABORATORY CO.,LTD. Registration number: Y2022990000023 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20221019 Address after: 214028 A601-3, 530 Building, No. 18 Qingyuan Road, Xinwu District, Wuxi, Jiangsu Province Patentee after: Zhide Technology (Wuxi) Co.,Ltd. Address before: 101300 second floor, building 37, lanxiyuan District 3, Renhe Town, Shunyi District, Beijing Patentee before: BEIJING ZHIDE MEDICAL LABORATORY CO.,LTD. |
|
TR01 | Transfer of patent right |