CN105854963A - Micro-channel structure and micro-fluid chip for achieving two-dimensional fluid power accumulation by using single-way sheath liquid - Google Patents
Micro-channel structure and micro-fluid chip for achieving two-dimensional fluid power accumulation by using single-way sheath liquid Download PDFInfo
- Publication number
- CN105854963A CN105854963A CN201610210300.1A CN201610210300A CN105854963A CN 105854963 A CN105854963 A CN 105854963A CN 201610210300 A CN201610210300 A CN 201610210300A CN 105854963 A CN105854963 A CN 105854963A
- Authority
- CN
- China
- Prior art keywords
- runner
- stream
- stream runner
- focusing
- longitudinal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a micro-channel structure and a micro-fluid chip with the micro-channel structure for achieving two-dimensional fluid power accumulation by using a single-way sheath liquid. The micro-channel structure comprises a sample flow channel, an upper longitudinal accumulated flow channel, a lower longitudinal accumulated flow channel and two transverse accumulated flow channels, wherein the upper longitudinal accumulated flow channel is arranged above the sample flow channel in an overlapped manner; the lower longitudinal accumulated flow channel is arranged below the sample flow channel in the overlapped manner; the two transverse accumulated flow channels are respectively arranged on the left side and right side of the sample flow channel; the width of each transverse accumulated flow channel is gradually reduced from back to front in a liquid flowing direction; and the maximum width of each transverse accumulated flow channel is greater than that of the sample flow channel. According to the micro-channel structure for achieving two-dimensional fluid power accumulation by using the single-way sheath liquid, disclosed by the embodiment of the invention, two-dimensional accumulation of a sample flow can be achieved, the sample flow can be prevented from being dispersed by the sheath liquid, the liquid flow can be kept stable, and the two-dimensional accumulation effect of the sample flow can be ensured.
Description
Technical field
The present invention relates to microfluidic channel technical field, utilize single channel sheath fluid to realize two dimensional fluid more particularly, to one and move
Power focus on micro-channel structure and be micro-fluid chip.
Background technology
In conjunction with flow cytometry, micro-fluid chip is commonly used for detecting cell, embryo, RNA, DNA, protein particulate, micro-
The biological particle such as biological, viral.In detection, micro-fluid chip needs stably to be focused on by the sample stream comprising biological particle
The center of fluid channel, to improve accuracy, sensitivity and the stability of detection.It is limited to process technology, fluid channel energy
It is only capable of planar realizing the curvilinear structures of complexity, but mostly is vertical tube wall or by inclining that processing technology determines in vertical direction
Tiltedly or bowl-type tube wall.Therefore, the micro-channel structure in correlation technique can complete paired samples stream transverse focusing in horizontal plane,
But it is difficult to the longitudinal focusing of vertical direction and has comprised the two-dimension focusing of both direction simultaneously, being both difficult to gather sample stream
Burnt in the center of fluid channel.Although some design structure is capable of two-dimension focusing, but flow velocity is relatively low, sample stream focuses on
Rear sectional area is relatively big, if center flow velocity is some tens of pm less than 1m/s, the sectional area length of side, and the center of business flow cytometer
Flow velocity mostly be 3-10m/s, biological particle yardstick many below 10 μm or even less.Thus, these designs cannot realize
High speed detection to biological particle, reduces the degree of accuracy and the stability of detection simultaneously, additionally, some micro-channel structure is permissible
Realize two-dimension focusing, and possess the feature of high flow rate, small bore.
Summary of the invention
It is contemplated that one of technical problem solved the most to a certain extent in correlation technique.To this end, the present invention proposes one
Planting the micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus, this canaliculus flow structure can utilize single channel sheath fluid real
The function of existing two-dimension focusing, simple in construction.
The present invention also proposes a kind of miniflow with the above-mentioned micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus
Body chip.
The single channel sheath fluid that utilizes of embodiment realizes the micro-channel structure of two dimensional fluid dynamic focus according to a first aspect of the present invention, bag
Including: sample stream runner, described sample stream runner linearly extends at fore-and-aft direction, and the two ends of described sample stream runner form sample
This inflow entrance and fluid outlet；Upper strata longitudinal focusing stream runner, described upper strata longitudinal focusing stream runner is folded is located at described sample stream
The top of runner and with described sample stream flow passage；Lower floor's longitudinal focusing stream runner, described lower floor longitudinal focusing stream runner is folded
Be located at described sample stream runner lower section and with described sample stream flow passage；Two transverse focusing stream runners, said two is horizontal
Be respectively provided at left side and the right side of described sample stream runner to focused flow runner, and respectively with described sample stream runner, described on
Layer longitudinal focusing stream runner and described lower floor longitudinal focusing stream flow passage, each described transverse focusing stream runner is in liquid flow direction
Above, being gradually reduced from rear to front width, the Breadth Maximum of each described transverse focusing stream runner is more than described sample stream runner
Width.
The single channel sheath fluid that utilizes according to embodiments of the present invention realizes the micro-channel structure of two dimensional fluid dynamic focus, by two laterally
Focused flow runner is located at the left and right sides of sample focused flow runner, makes sample stream can first realize transverse focusing, i.e. laterally
The size of compression samples stream on direction, is respectively provided at corresponding position by upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream runner
Above and below the transverse focusing stream runner put, make sample stream can realize longitudinal focusing, i.e. when upper strata longitudinal focusing stream
Sheath liquid flow in runner and lower floor's longitudinal focusing stream runner enters the vertical of the sample stream runner compressible sample stream in intermediate layer symmetrically
To size, thus realize the two-dimension focusing of sample stream on the whole, owing to the flow of sheath liquid flow is much larger than the flow of sample stream,
The width of transverse focusing stream runner is set greater than the width of sample stream runner, it can be ensured that when sample stream and sheath liquid flow cross,
Speed/the dynamic pressure of sample stream is slightly larger than sheath liquid flow, prevents sample stream from being broken up by sheath liquid flow, keeps liquid flow stable, it is ensured that sample stream
Two-dimension focusing effect.
It addition, the single channel sheath fluid that utilizes according to embodiments of the present invention realizes the micro-channel structure of two dimensional fluid dynamic focus, also may be used
To have following additional technical characteristic:
According to one embodiment of present invention, said two transverse focusing stream runner is about the longitudinal center of described sample stream runner
Cross section is symmetrical.
According to one embodiment of present invention, it is characterised in that described upper strata longitudinal focusing stream runner and described lower floor are the most poly-
Burnt stream runner is symmetrical above and below about the transverse center cross section of described sample stream runner.
According to one embodiment of present invention, described upper strata longitudinal focusing stream runner and/or described lower floor longitudinal focusing stream runner bag
Include: line flowing channel portion, described line flowing channel portion and described sample stream runner alignment in the vertical direction；Left side camber line stream
Road portion, described left side camber line flow path portion is located at the left side in described line flowing channel portion, and the front end of described left side camber line flow path portion with
The rear end in described line flowing channel portion is tangent and connects；Right side camber line flow path portion, described right side camber line flow path portion is located at described straight line
The right side of flow path portion, and the front end of described right side camber line flow path portion is tangent with the rear end in described line flowing channel portion and connects, described
The outboard wheel profile of upper strata longitudinal focusing stream runner and described lower floor longitudinal focusing stream runner and said two transverse focusing stream runner
Opposing outboard wheel profile alignment in the vertical direction.
According to one embodiment of present invention, the front end of described left side camber line flow path portion and the front end of described right side camber line flow path portion
Angle between connection, and the nearside wheel profile of described left side camber line flow path portion and described right side camber line flow path portion intersection is α,
50°≤α≤80°。
According to one embodiment of present invention, the front end face in described line flowing channel portion forms forwardly convex non-of plane or middle part
Plane.
According to one embodiment of present invention, said two transverse focusing stream runner forms the arc of opposing setting respectively, and often
The width of individual described transverse focusing stream runner is more than described left side camber line flow path portion and the width of described right side camber line flow path portion.
According to one embodiment of present invention, the opposing outboard wheel profile of two described transverse focusing stream runners is interior with relative
Side wheel profile respectively with the tangent setting of outer contour of described sample stream runner.
According to one embodiment of present invention, the longitudinal thickness of described sample stream runner and two described transverse focusing stream runners
Longitudinal thickness is equal, and the longitudinal thickness of described upper strata longitudinal focusing stream runner and described lower floor longitudinal focusing stream runner is equal.
According to one embodiment of present invention, the longitudinal thickness of described sample stream runner and described upper strata longitudinal focusing stream runner/
The longitudinal thickness of described lower floor longitudinal focusing stream runner is equal.
According to one embodiment of present invention, the longitudinal thickness of described sample stream runner and described upper strata longitudinal focusing stream runner/
The longitudinal thickness of described lower floor longitudinal focusing stream runner is unequal.
According to one embodiment of present invention, also include two focused flow entrances, said two focused flow entrance respectively with two
Described transverse focusing stream runner, described upper strata longitudinal focusing stream runner and described lower floor longitudinal focusing stream flow passage, described two
Individual focused flow entrance is connected by pipeline and a liquid flowing source.
The micro-fluid chip of embodiment according to a second aspect of the present invention, including according to utilizing single channel sheath fluid described in above-described embodiment
Realize the micro-channel structure of two dimensional fluid dynamic focus.
According to one embodiment of present invention, including: the first lamellar body, described first lamellar body is formed described sample stream runner
With two described transverse focusing stream runners；Second lamellar body, described second lamellar body folds the top being located at described first lamellar body, and institute
State and on the second lamellar body, be formed with described upper strata longitudinal focusing stream runner；3rd lamellar body, described 3rd lamellar body is folded is located at described first
Described lower floor longitudinal focusing stream runner it is formed with on the lower section of lamellar body, and described 3rd lamellar body；Upper cover, described upper cover is folded to be located at
The top of described second lamellar body；Lower cover, described lower cover folds the lower section being located at described 3rd lamellar body, described upper cover and described lower cover
In at least one be provided with sample streamer entrance that focused flow inlet connects with described sample inflow entrance and go out with described liquid flow
The delivery outlet of mouth connection.
The additional aspect of the present invention and advantage will part be given in the following description, and part will become bright from the following description
Aobvious, or recognized by the practice of the present invention.
Accompanying drawing explanation
Fig. 1 is the structural representation of micro-channel structure according to an embodiment of the invention；
Fig. 2 is the explosive view of the micro-channel structure shown in Fig. 1；
Fig. 3 is structural representation and the cross-sectional view of diverse location of micro-channel structure according to embodiments of the present invention；
Fig. 4 is the structural representation of the micro-channel structure according to another embodiment of the present invention；
Fig. 5 is the top view of the micro-channel structure shown in Fig. 1；
Fig. 6 is structural representation and the partial enlarged drawing of the micro-channel structure shown in Fig. 1；
Fig. 7 is structural representation and the cross-sectional view of the micro-channel structure shown in Fig. 1；
The stereogram of Fig. 8 micro-fluid chip according to embodiments of the present invention；
Fig. 9 is the explosive view of the structure shown in Fig. 8.
Reference:
A: sample stream；B: sheath liquid flow；C: liquid flowing source；
1: micro-fluid chip；
100: micro-channel structure；
111: sample stream runner；111a: sample inflow entrance；111b: fluid outlet；
112: transverse focusing stream runner；112a: focused flow entrance；
120: upper strata longitudinal focusing stream runner；
121 (131): line flowing channel portion；
122 (132): left side camber line flow path portion；
123 (133): right side camber line flow path portion；
130: lower floor's longitudinal focusing stream runner；
210: the first lamellar bodies；220: the second lamellar bodies；230: the three lamellar bodies；240: upper cover；241: sample streamer entrance；
242: delivery outlet；243: focused flow inlet；244: through hole；245: watch window；250: lower cover.
Detailed description of the invention
Embodiments of the invention are described below in detail, and the example of described embodiment is shown in the drawings.Below with reference to accompanying drawing
The embodiment described is exemplary, it is intended to is used for explaining the present invention, and is not considered as limiting the invention.
1 to Fig. 7 specifically describes the single channel sheath fluid that utilizes of embodiment according to a first aspect of the present invention and realizes two dimension below in conjunction with the accompanying drawings
The micro-channel structure 100 of Hydrodynamic focus.
As it is shown in figure 1, the single channel sheath fluid that utilizes according to embodiments of the present invention realizes the micro-channel structure of two dimensional fluid dynamic focus
100 include that 111, two transverse focusing stream runners 112 of sample stream runner, upper strata longitudinal focusing stream runner 120 and lower floor are longitudinally
Focused flow runner 130.Specifically, sample stream runner 111 linearly extends at fore-and-aft direction, sample stream runner 111
Two ends form sample inflow entrance 111a and fluid outlet 111b, and upper strata longitudinal focusing stream runner 120 is folded is located at sample stream runner
The top of 111 and connecting with sample stream runner 111, lower floor's longitudinal focusing stream runner 130 is folded is located at sample stream runner 111
Lower section and connect with sample stream runner 111, two transverse focusing stream runners 112 are respectively provided at the left side of sample stream runner 111
And right side, and respectively with sample stream runner 111, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
Connection, each transverse focusing stream runner 112 on liquid flow direction, be gradually reduced from rear to front width, each transverse focusing stream
The Breadth Maximum of runner 112 is more than the width of sample stream runner 111.
Alternatively, 111, two transverse focusing stream runners 112 of the sample stream runner of this micro-channel structure 100, upper strata are longitudinally gathered
Burnt stream runner 120 and lower floor's longitudinal focusing stream runner 130 can be limited by the housing of micro-channel structure 100, it is preferable that
Sample stream runner 111 forms the linearity extended along the longitudinal direction, and the cross section that sample stream runner 111 is on diverse location
Shape is identical, area of section is equal, and wherein, the rear end of sample stream runner 111 forms sample inflow entrance 111a, sample stream stream
The front end in road 111 forms fluid outlet 111b, and sample stream runner 111 may be used for circulating sample stream a and focusing on liquid (such as
Sheath liquid flow b).
Two transverse focusing stream runners 112 are respectively provided at the left and right sides of sample stream runner 111 and two transverse focusing stream runners
112 connect with sample stream runner 111 respectively, and sample stream a enters in sample stream runner 111 from sample inflow entrance 111a, sheath
Liquid flow b enters the transverse focusing stream runner 112 of the left and right sides, upper strata longitudinal focusing stream runner 120 from focused flow entrance 112a
With lower floor longitudinal focusing stream runner 130, first sample stream a and is positioned at the transverse focusing stream of sample stream runner 111 left and right sides
Sheath liquid flow b contact in runner 112, along with transverse focusing stream runner 112 gradually narrows, sheath liquid flow b is symmetrically squeezed
Pressure-like this stream a, holding sample stream a are in center, left and right and reduce its lateral dimension, it is achieved that to sample stream a's
Transverse focusing (focusing of left and right directions).
Upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 are respectively provided at the upper and lower of sample stream runner 111
Both sides, and upper strata longitudinal focusing stream runner 120 is positioned at the top of two transverse focusing stream runners 112 and laterally poly-with two
Burnt stream runner 112 connects, and lower floor's longitudinal focusing stream runner 130 is positioned at the lower section of two transverse focusing stream runners 112 and with two
Individual transverse focusing stream runner 112 connects, and is positioned at the upper strata longitudinal focusing stream runner 120 above and below sample stream runner 111
Extrude liquid flow (the sample stream a being positioned at middle part runner symmetrical above and belowly with sheath liquid flow b in lower floor longitudinal focusing stream runner 130
With sheath liquid flow b), it is ensured that it is in upper and lower center, it is achieved thereby that to the longitudinal focusing of sample stream a (above-below direction
Focus on).Subsequently, sample stream a enters the leading portion of sample stream runner 111 under the parcel of sheath liquid flow b, and is stably positioned in sample
The central cross-section position of this stream runner 111, it is simple to sample stream a is carried out all kinds of Photoelectric Detection.
It is to say, this micro-channel structure 100 is three layers of flow passage structure, three laminar flow roads are interconnected and not Presence of an interface, its
In, sample stream runner 111 and the transverse focusing stream runner 112 being located at sample stream runner 111 left and right sides are positioned at middle level, with
Sheath liquid flow b in two transverse focusing stream runners 112 in rear to front flowing, two transverse focusing stream runners 112
Sheath liquid flow b can realize the transverse focusing to sample stream a, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream stream
Road 130 lays respectively at the upper and lower, and the end (front end) at two longitudinal focusing stream runners is incorporated to be positioned at the sample in middle level
Stream runner 111, along with two longitudinal focusing stream runners (upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner
130) thickness reduces, and sheath liquid flow b in two longitudinal focusing stream runners is incorporated in sample stream runner 111, it is possible to achieve right
The longitudinal focusing of sample stream a.
Thus, the single channel sheath fluid that utilizes according to embodiments of the present invention realizes the micro-channel structure 100 of two dimensional fluid dynamic focus,
Two transverse focusing stream runners 112 are located at the left and right sides of sample focused flow runner, make sample stream a can first realize horizontal stroke
To focusing, i.e. in the size of the upper compression samples stream a of horizontal direction (left and right directions as shown in Figure 1), upper strata is the most poly-
The transverse focusing stream runner 112 that burnt stream runner 120 and lower floor's longitudinal focusing stream runner 130 are respectively provided on correspondence position upper
Side and lower section, make sample stream a can realize longitudinal focusing, i.e. when upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing
Sheath liquid flow b in stream runner 130 enters the longitudinal size of the sample stream runner 111 compressible sample stream a in intermediate layer symmetrically,
Thus realize the two-dimension focusing of sample stream a on the whole, and owing to the flow of sheath liquid flow b is much larger than the flow of sample stream a, will
The width of transverse focusing stream runner 112 is set greater than the width of sample stream runner 111, it can be ensured that sample stream a and sheath fluid
Stream b when crossing, sample stream a speed/dynamic pressure slightly larger than sheath liquid flow b, prevent sample stream a from being broken up by sheath liquid flow b, keep
Liquid flow is stable, it is ensured that the two-dimension focusing effect of sample stream a.
Preferably, according to one embodiment of present invention, two transverse focusing stream runners 112 are about sample stream runner 111
Longitudinal center cross section is symmetrical.Upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 are about sample stream
The transverse center cross section of runner 111 is symmetrical above and below.
It is to say, the micro-channel structure 100 of the embodiment of the present invention is mainly made up of three layers of fluid channel, present a left side on the whole
Right and symmetrical above and below, up/down perforation, not Presence of an interface between three layers of fluid channel, ground floor fluid channel above (is indulged by upper strata
To focused flow runner 120) structure and be positioned at the structure of third layer fluid channel (lower floor's longitudinal focusing stream runner 130) of lower section
Identical, the structure of two the transverse focusing stream runners 112 being positioned at centre is identical.During work, sample stream a is from sample inflow entrance
111a enters in the vertical direction, is positioned at the sample stream runner 111 of centre, and sheath liquid flow b is entered from focused flow entrance 112a
Enter the transverse focusing stream runner 112 of the left and right sides, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
In, sample stream a realizes transverse focusing and longitudinal focusing under the effect of sheath liquid flow b successively, i.e. realizes two-dimension focusing.Thus,
By by right to two transverse focusing stream runners 112, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
Claim be arranged in the left and right of sample stream runner 111, upper and lower orientation, it can be ensured that the sheath liquid flow b pressure of longitudinal focusing stream runner with
Flow stream pressure in transverse focusing stream runner 112 synchronizes change, maintains liquid flow stable, thus ensures that the two dimension of sample stream a is gathered
Burnt effect.
Wherein, as in figure 2 it is shown, according to one embodiment of present invention, upper strata longitudinal focusing stream runner 120 and/or lower floor indulge
Line flowing channel portion 121 (131), left side camber line flow path portion 122 (132) and right side camber line stream is included to focused flow runner 130
Road portion 123 (133).Specifically, align in the vertical direction with sample stream runner 111 in line flowing channel portion 121 (131)
Arranging, left side camber line flow path portion 122 (132) is located at the left side in line flowing channel portion 121 (131), and left side camber line flow path portion
The front end of 122 (132) is tangent with the rear end in line flowing channel portion 121 and connects, and right side camber line flow path portion 123 (133) is located at
The right side in line flowing channel portion 121 (131), and the front end of right side camber line flow path portion 123 (133) and line flowing channel portion 121 (131)
Rear end tangent and connection, the outboard wheel profile of upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 with
The outboard wheel profile alignment in the vertical direction that two transverse focusing stream runners 112 are opposing.
In other words, upper strata longitudinal focusing stream runner 120 is main by the line flowing channel portion 121 being positioned at above sample stream runner 111
Form with the left side camber line flow path portion 122 being positioned at above two transverse focusing stream runners 112 and right side camber line flow path portion 123,
Lower floor's longitudinal focusing stream runner 130 is mainly by the line flowing channel portion 131 being positioned at below sample stream runner 111 be positioned at two horizontal strokes
Left side camber line flow path portion 132 and right side camber line flow path portion 133 below focused flow runner 112 form.Wherein, it is positioned at
The left side camber line flow path portion 122 of side and right side camber line flow path portion 123 are tangent with line flowing channel portion 121 above respectively to be set
Put and connect and respectively with the position one_to_one corresponding of two transverse focusing stream runners 112, be positioned at the left side camber line flow path portion of lower section
132 with right side camber line flow path portion 133 respectively be positioned at lower section the tangent setting in line flowing channel portion 131 and connect and respectively with two
The position one_to_one corresponding of individual transverse focusing stream runner 112.
During work, sample stream a enters in sample stream runner 111 from sample inflow entrance 111a, and sheath liquid flow b is from focused flow entrance
112a enters the transverse focusing stream runner 112 of the left and right sides, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream stream
In road 130, first, sample stream a and be positioned at the sheath of transverse focusing stream runner 112 of sample stream runner 111 left and right sides
Liquid flow b contacts, and along with transverse focusing stream runner 112 gradually narrows, sheath liquid flow b symmetrically extrudes sample stream a, guarantor
Hold it be in center, left and right and reduce its lateral dimension, it is achieved that the transverse focusing to sample stream a.Subsequently, it is positioned at
Upper strata longitudinal focusing stream runner 120 above and below sample stream runner 111 and the sheath in lower floor's longitudinal focusing stream runner 130
Liquid flow b extrudes liquid flow (sample stream a and the sheath liquid flow b), it is ensured that it is in upper and lower center being positioned at middle part runner symmetrical above and belowly
Position, along with the reduction of line flowing channel portion 121 (131) longitudinal size, it is achieved that the longitudinal focusing to sample stream a.Sample
Stream a is focused in horizontal and vertical both direction, completes two-dimension focusing.
Subsequently, the periphery of sample stream a enters the leading portion of sample stream runner 111 under the parcel of sheath liquid flow b, and stablizes status
Central cross-section position in sample stream runner 111, it is simple to sample stream a is carried out all kinds of Photoelectric Detection.In operation, enter a left side
The upper strata longitudinal focusing stream runner 120 of the transverse focusing stream runner 112 of right both sides and up and down both sides and lower floor's longitudinal focusing stream
Sheath liquid flow b of runner 130 is symmetrical, thus sheath liquid flow b can be driven by a power source, i.e. utilizes single channel sheath fluid to realize
Two dimensional fluid dynamic focus, by choosing different structural parameters and regulation liquid flow, the miniflow of the embodiment of the present invention
Sample stream a can be focused to several microns to more than 20 microns (sample stream under the center flow velocity of several metre per second (m/s)s by road structure 100
The characteristic size in a cross section), it is achieved that the effective focusing under high flow rate, it is possible to meet high speed detection demand.
Thus, transverse focusing stream runner 112, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 phase
Mutually conducting, and (upper strata is vertical with two longitudinal focusing stream runners by the opposing lateral profile of two transverse focusing stream runners 112
To focused flow runner 120 and lower floor's longitudinal focusing stream runner 130) opposing lateral profile is arranged to identical, it can be ensured that vertical
To the change Tong Bu with the flow stream pressure in transverse focusing stream runner 112 of the sheath liquid flow b pressure of focused flow runner, maintain liquid flow steady
Fixed.
Alternatively, according to one embodiment of present invention, the front end of left side camber line flow path portion 122 (132) and right side camber line stream
The front end connection in road portion 123 (133), and left side camber line flow path portion 122 (132) and right side camber line flow path portion 123 (133)
Angle between the nearside wheel profile of intersection A is α, 50 °≤α≤80 °.
With reference to Fig. 5, the left side camber line flow path portion 122 of upper strata longitudinal focusing stream runner 120 and right side camber line flow path portion 123 from
Gradually cross to front and make its front end connect with the rear end in the line flowing channel portion 121 of upper strata longitudinal focusing stream runner 120 afterwards, under
Left side camber line flow path portion 132 and the right side camber line flow path portion 133 of layer longitudinal focusing stream runner 130 gradually cross also from rear to front
Its front end is made to connect with the line flowing channel portion 131 of lower floor longitudinal focusing stream runner 130.
Wherein, left side camber line flow path portion 122 (132) and the inner side profile of right side camber line flow path portion 123 (133) intersection A
Angle between line controls between 50 ° to 80 °, such as 50 °, 60 ° or 80 °, so can ensure that left side arc
Line flow path portion 122 (132) has certain length L with the intersection A of right side camber line flow path portion 123 (133), by experiment
Understand: with the runner of left side camber line flow path portion 122 (132) in layer with the intersection A of right side camber line flow path portion 123 (133)
Profile needs to meet particular/special requirement: this intersection A profile keeps suitable length L at fore-and-aft direction needs；If intersection A takes turns
Wide long sheath liquid flow b that can increase in upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 of length L
To the transverse shear stress of sample stream a, weaken longitudinal extruding, thus increase the height dimension in cross section after sample stream a focuses on；If handing over
At remittance, the too short meeting of length L of A profile makes left side camber line flow path portion 122 (132) and right side camber line flow path portion 123 (133)
In sheath liquid flow cannot cross in time due to effect of inertia, and intersection A produce local depression, make the second layer micro-simultaneously
Liquid flow in runner (sample stream runner 111 and two transverse focusing stream runners 112) is to upper strata longitudinal focusing stream runner 120
Extend with in lower floor longitudinal focusing stream runner 130, the height dimension in the cross section after sample stream a focuses on can be increased equally.
Thus, by by left side camber line flow path portion 122 (132) and right side camber line flow path portion 123 (133) intersection A profile
Wire clamp angle controls between 50 ° to 80 °, it is ensured that be positioned at the left side camber line flow path portion 122 above sample stream runner 111
With right side camber line flow path portion 123, be positioned at the left side camber line flow path portion 132 below sample stream runner 111 and right side camber line runner
Intersection of portion 133 A has suitable length L so that left side camber line flow path portion 122 (132) and right side camber line flow path portion 123
(133) sheath liquid flow b in along with the two cross, in the vertical direction, the left and right sides is coated with the sample of sheath liquid flow b
Stream a extrudes, and with stable two femoral sheath liquid flows b merged with sample stream a effectively, contributing to promoting downstream will be carried out
The longitudinal focusing to sample stream a, thus ensure the sheath liquid flow b longitudinal focusing effect to sample stream a.
Alternatively, as shown in B portion in Fig. 6, according to one embodiment of present invention, the front end face shape in line flowing channel portion 121
Become the forwardly convex on-plane surface of plane or middle part.During work, it is positioned at the upper strata above two transverse focusing stream runners 112 and indulges
Sheath liquid flow b in the left side camber line flow path portion 122 and right side camber line flow path portion 123 of focused flow runner 120 crosses at the two
End, entrance are positioned at the line flowing channel portion 121 above sample stream runner 111, are positioned at two transverse focusing stream runners 112 times
Sheath liquid flow b in the left side camber line flow path portion 132 of lower floor's longitudinal focusing stream runner 130 of side and right side camber line flow path portion 133
It is positioned at the line flowing channel portion 131 below sample stream runner 111 in the two end that crosses, entrance.
In some concrete example examples of the present invention, the front end face in the line flowing channel portion 121 (131) of upper and lower both sides is formed
Plane, can play the effect of longitudinal focusing to sample stream a；In other concrete examples of the present invention, by two straight lines
The front end face of flow path portion 121 (131) can form the on-plane surface that middle part is forwardly convex, if such as line flowing channel portion 121 (131)
Front end face formed in the middle part of forwardly convex and symmetrical folded angular surface, i.e. form two crossing planes, such sheath liquid flow b is one
Can be formed in the cross section of fluid channel of segment distance four symmetrical, there is the eddy current of longitudinal compression effect, these eddy current further enhance
Longitudinal focusing effect to sample stream a.
Preferably, as it is shown in fig. 7, by the front end face in the line flowing channel portion 121 of upper strata longitudinal focusing stream runner 120 and lower floor
The front end face in the line flowing channel portion 131 of longitudinal focusing stream runner 130 is respectively formed the arcwall face that middle part is forwardly convex, such sheath fluid
Stream b formed in the cross section of fluid channel of a segment distance four that be respectively symmetrically at above-below direction and left and right directions, there is longitudinal compression
The eddy current of effect, in the process, along with upper strata longitudinal focusing stream runner 120 and the thickness of lower floor's longitudinal focusing stream runner 130
The reduction of degree, the longitudinal size of sheath liquid flow b compression samples stream a, thus realize the longitudinal focusing to sample stream a, furthermore,
Four eddy current of sheath liquid flow b can further enhance the longitudinal focusing to sample stream a, it is ensured that the effect of the longitudinal focusing of sample stream a
Really.Specifically, the desired size after can focusing on according to different flows and sample stream a, choose different end profile
Realize.
Thus, forwardly convex by the front end face in line flowing channel portion 121 (131) being set to middle part on-plane surface, it is possible to make
Sheath liquid flow b forms four eddy current symmetrical, that have longitudinal compression effect, these whirlpools in the cross section of sample stream runner 111
Stream can further enhance the longitudinal focusing effect to sample stream a.
Alternatively, according to one embodiment of present invention, two transverse focusing stream runners 112 form the arc of opposing setting respectively
Shape, and the width of each transverse focusing stream runner 112 is more than left side camber line flow path portion 122 (132) and right side camber line flow path portion
The width of 123 (133).
In other words, sample stream runner 111 forms the straight channel extended along the longitudinal direction, and two transverse focusing stream runners 112 set
Two arc fluid channel crossed, position is formed in the left and right sides of sample stream runner 111 and two transverse focusing stream runners 112
The left side camber line flow path portion 122 of the upper strata longitudinal focusing stream runner 120 above sample stream runner 111 and right side camber line runner
Portion 123, the left side camber line flow path portion 132 being positioned at lower floor's longitudinal focusing stream runner 130 below sample stream runner 111 and the right side
Side camber line flow path portion 133 forms the arc fluid channel corresponding with the appearance profile of two transverse focusing stream runners 112 respectively.Will
The width of transverse focusing stream runner 112 is set greater than left side camber line flow path portion 132 and the width of right side camber line flow path portion 133,
First sheath liquid flow b in two transverse focusing stream runners 112 is gradually crossed on liquid flow direction, during work, is positioned at sample
First sheath liquid flow b in the transverse focusing stream runner 112 of the left and right sides of this stream runner 111 contacts with sample stream a, along with
Transverse focusing stream runner 112 gradually narrows, and sheath liquid flow b symmetrically extrudes sample stream a, maintains it in center, left and right
Position also reduces its lateral dimension, it is achieved that the transverse focusing to sample stream a.Then upper strata longitudinal focusing stream runner 120
Extrude sample stream a, it is achieved sample stream a is indulged symmetrical above and belowly with sheath liquid flow b in lower floor longitudinal focusing stream runner 130
To focusing.
Thus, two transverse focusing stream runners 112 are symmetrically arranged at the left and right sides of sample stream runner 111, with by sample stream
A focuses on center position, introduces sheath liquid flow b, it is possible to achieve to sample in symmetrical transverse focusing stream runner 112
The transverse focusing of stream a；Flow due to sheath liquid flow b is much larger than the flow of sample stream a, by transverse focusing stream runner 112
Width is set greater than the width of sample stream runner 111, it can be ensured that when sample stream a and sheath liquid flow b cross, sample stream a
Speed/dynamic pressure slightly larger than sheath liquid flow b, prevent sample stream a from being broken up by sheath liquid flow b.Width by transverse focusing stream runner 112
Degree is set greater than left side camber line flow path portion 122 (132) or the width of right side camber line flow path portion 123 (133), is conducive to
Ensure that first sample stream a carries out transverse focusing, until in the case of transverse focusing is stable, realizing longitudinally gathering again to sample stream a
Jiao, improves the two-dimension focusing effect of sample stream a.
Preferably, according to one embodiment of present invention, the opposing outboard wheel profile of two transverse focusing stream runners 112 and
Relative nearside wheel profile respectively with the tangent setting of outer contour of sample stream runner 111.Thus, two transverse focusing stream stream
Road 112 is tangent with sample stream runner 111, slowly integrate the sample stream runner 111, Ke Yiyue of centre in the longitudinal direction
Bundle-sheath liquid flow b is focusing on and retaining layer stream mode in steering procedure, is flowing to smooth change, and streamline, without sudden change, thus reduces stream
Dynamic resistance, the raising stability of a system.The distance that crosses longer between transverse focusing stream runner 112 and sample stream runner 111,
Can make sample stream a before crossing with sheath liquid flow b in longitudinal focusing stream runner, reach stable transverse focusing state, protect
The stability of card liquid flow.
Wherein, according to one embodiment of present invention, the longitudinal thickness of sample stream runner 111 and two transverse focusing stream runners
The longitudinal thickness of 112 is equal, upper strata longitudinal focusing stream runner 120 and the longitudinal thickness phase of lower floor's longitudinal focusing stream runner 130
Deng.Thus ensure the stability of transverse focusing, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
Longitudinal thickness is equal, thus ensures the stability of longitudinal focusing.
In certain specific embodiments of the invention, the longitudinal thickness of sample stream runner 111 and upper strata longitudinal focusing stream runner
The longitudinal thickness of 120/ lower floor's longitudinal focusing stream runner 130 is equal.In other words, two transverse focusing stream runners 112 it are positioned at
The upper strata longitudinal focusing stream runner 120 of top and lower floor's longitudinal focusing stream of the lower section being positioned at two transverse focusing stream runners 112
Runner 130 thickness in the vertical direction is equal, and the thickness of upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing
The thickness of stream runner 130 is equal, at miniflow with the thickness of 111, two transverse focusing stream runners 112 of sample stream runner respectively
Road structure 100 on the whole, the thickness of three layers of fluid channel equal, can meet and sample stream a is focused on wanting of vertical centre
Ask.
In other detailed description of the invention of the present invention, the longitudinal thickness of sample stream runner 111 and upper strata longitudinal focusing stream stream
The longitudinal thickness of road 120/ lower floor longitudinal focusing stream runner 130 is unequal.In other words, two transverse focusing stream runners 112 it are positioned at
Lower floor's longitudinal focusing of upper strata longitudinal focusing stream runner 120 and the lower section being positioned at two transverse focusing stream runners 112 of top
Stream runner 130 thickness in the vertical direction is equal, and the thickness of upper strata longitudinal focusing stream runner 120 and lower floor longitudinally gather
The thickness of burnt stream runner 130 is unequal with the thickness of 111, two transverse focusing stream runners 112 of sample stream runner respectively,
Micro-channel structure 100 on the whole, the thickness of the fluid channel of above and lower section be positioned at middle part fluid channel thickness not
Equal, by adjusting the thickness of longitudinal focusing stream runner, sheath liquid flow b can be increased or decreased in longitudinal focusing stream runner
Flow, thus strengthen or weaken the longitudinal focusing effect to sample stream a.
Wherein, according to one embodiment of present invention, two focused flow entrance 112a, two focused flow entrance 112a are also included
Respectively with two transverse focusing stream runners 112, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 even
Logical, two focused flow entrance 112a are connected by pipeline and a liquid flowing source c.
Specifically, as it is shown in figure 1, be positioned at the rear end of transverse focusing stream runner 112 in the left side of sample stream a, upper strata longitudinally
Behind the rear end of the left side camber line flow path portion 122 of 120 and the left side camber line flow path portion 132 of lower floor's longitudinal focusing stream runner 130
End formed a focused flow entrance 112a, i.e. this focused flow entrance 112a simultaneously be positioned at left side transverse focusing stream runner 112,
The rear end conducting of upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130, is positioned at the horizontal stroke on the right side of sample stream a
To the rear end of focused flow runner 112, upper strata longitudinally 120 the rear end of right side camber line flow path portion 123 and lower floor's longitudinal focusing stream
The rear end of the right side camber line flow path portion 133 of runner 130 forms another focused flow entrance 112a, i.e. this focused flow entrance 112a
Simultaneously with transverse focusing stream runner 112, upper strata longitudinal focusing stream runner 120 and the lower floor's longitudinal focusing stream runner being positioned at right side
The rear end conducting of 130.
Wherein, sheath liquid flow b can by a power source be driven through two focused flow entrance 112a enter symmetrical laterally
In focused flow runner 112 and laterally zygomorphic upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130, i.e.
Single channel sheath fluid is utilized to realize two dimensional fluid dynamic focus, it is ensured that sheath liquid flow b symmetrical.This micro-channel structure 100 can
Under the center flow velocity of many metres per second, sample stream a is focused to the characteristic size in its cross section several for micron to more than 20 microns, i.e.
The effective focusing under high flow rate can be realized.
Below in conjunction with multiple embodiments specifically describe according to embodiments of the present invention utilize single channel sheath fluid realize two dimensional fluid power gather
Burnt micro-channel structure 100.
Micro-channel structure 100 on the whole, present left and right and symmetrical above and below, it is made up of three layers of fluid channel, three layers of fluid channel
Between up/down perforation, not Presence of an interface, wherein, second layer fluid channel is located between ground floor fluid channel and third layer fluid channel,
Ground floor is identical with the structure of third layer fluid channel, as it is shown on figure 3, ground floor fluid channel is upper strata longitudinal focusing stream runner 120,
Its at the middle and upper levels longitudinal focusing stream runner 120 include line flowing channel portion 121 and two left side camber line flow path portion 122 Hes crossed
Right side camber line flow path portion 123, third layer fluid channel is lower floor's longitudinal focusing stream runner 130, wherein lower floor's longitudinal focusing stream runner
130 include line flowing channel portion 131 and two left side camber line flow path portion 132 crossed and right side camber line flow path portion 133, second
Layer fluid channel is mainly by a sample stream runner 111 in an intermediate position and two cambers importing sample stream runner 111
Transverse focusing stream runner 112 form, and the width of transverse focusing stream runner 112 is more than the width of sample stream runner 111；
In three layers of fluid channel, all of arc fluid channel has identical lateral profile.
During work, sample stream a enters from the sample inflow entrance 111a of sample stream runner 111, and sheath liquid flow b is from focused flow entrance
112a enters the arc fluid channel of the left and right sides and (includes the transverse focusing stream runner being positioned at sample stream runner 111 left and right sides
112, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130), sample stream a is in the effect of sheath liquid flow b
Under realize transverse focusing and longitudinal focusing, i.e. two-dimension focusing successively.First, sample stream a and two arc miniflows of the second layer
Sheath liquid flow b contact in road (transverse focusing stream runner 112), along with transverse focusing stream runner 112 gradually narrows, sheath fluid
Stream b symmetrically extrudes sample stream a, maintains it in center, left and right and reduce its lateral dimension, it is achieved that right
The transverse focusing of sample stream a, cross section of fluid channel as shown in Figure 3.
Subsequently, at the end (front end as shown in Figure 3) of transverse focusing stream runner 112, (upper strata is longitudinally for ground floor fluid channel
Focused flow runner 120) and third layer fluid channel (lower floor's longitudinal focusing stream runner 130) in sheath liquid flow b enter into the second layer
In fluid channel, extrude the liquid flow in second layer fluid channel symmetrical above and belowly, maintain it in upper and lower center and reduce it
Longitudinal size, it is achieved thereby that the longitudinal focusing to sample stream a.Sample stream a is focused in horizontal and vertical both direction,
Complete two-dimension focusing.Afterwards, sample stream a enters the leading portion of sample stream runner 111 under the parcel of sheath liquid flow b, and surely
Surely the center in sample stream runner 111 cross section it is positioned at, such as the cross section of fluid channel in Fig. 3, consequently facilitating sample stream a is entered
All kinds of Photoelectric Detection of row.Preferably, as shown in Figure 4, in operation, sheath liquid flow b of left and right sides arc fluid channel is entered
Symmetrical, thus sheath liquid flow b can i.e. be utilized single channel sheath fluid to achieve two dimensional fluid power gathered by a liquid flowing source c supply
Burnt.
Embodiment one
Ground floor fluid channel connects with the fluid channel with the second layer of third layer fluid channel, and has same lateral profile,
Change ensureing that sheath liquid flow b pressure therein is Tong Bu with the flow stream pressure in second layer fluid channel, and maintain liquid flow in system
Stable；Ground floor fluid channel (upper strata longitudinal focusing stream runner 120) or third layer fluid channel (lower floor's longitudinal focusing stream runner
130) in, two arc fluid channel (left side camber line flow path portion 122 (132) and right side camber line flow path portion 123 (133)) cross
The runner profile of position need to need to keep suitable length L at fore-and-aft direction, and length is long can increase ground floor fluid channel and the
Sheath liquid flow b in three layers of fluid channel to the transverse shear stress of sample stream a, weaken longitudinal extruding, increase after sample stream a focuses on and cut
The height dimension in face, length is too short, sheath liquid flow b in arc fluid channel cannot be crossed in time due to effect of inertia,
The liquid flow produced in local depression, second layer fluid channel at intersection A extends in ground floor fluid channel and third layer fluid channel,
Increase the height dimension in cross section after sample stream a focuses on, therefore, the left side camber line of the micro-channel structure 100 of the embodiment of the present invention
The runner profile of the position that crosses of flow path portion 122 (132) and right side camber line flow path portion 123 (133) uses-80 °, α=50 °
Structure, thus solve the problems referred to above.
Specifically, in the present embodiment, the height of second layer fluid channel and ground floor fluid channel and the height of third layer fluid channel
Equal, the height of three layers of fluid channel is all chosen for 150 μm, and the width of the sample stream runner 111 being positioned at the second layer is chosen for 300
In μm, ground floor fluid channel or third layer fluid channel, inside the runner of two arc fluid channel intersection A, profile angle is chosen for
70 °, the front end face being positioned at two the line flowing channel portions 121 (131) above and below sample stream runner 111 is arcwall face,
This micro-channel structure 100 is capable of focusing at a high speed, and after focusing, the flow velocity of sample stream a is up to several metre per second (m/s)s.
First, choose sample stream a flow be 0.5 μ L/s, sheath liquid flow b flow be that 120 μ L/s carry out two to sample stream a
Dimension focuses on experiment, and subsequently, the cross sectional shape after sample stream a focuses on is approximately the square of 12 μ m 12 μm (wide × high),
The flow velocity of sample stream a reaches 5.1m/s.
Choose sample stream a flow be 1 μ L/s, sheath liquid flow b flow be 100 μ L/s, subsequently sample stream a focus on after cross section
Shape is approximately the rectangle of 13 μ m 24 μm (wide × high), and now, sample stream a is positioned at the center of runner, sample stream a
Flow velocity reach 4.3m/s.
Choose sample stream a flow be 1 μ L/s, sheath liquid flow b flow be 120 μ L/s, subsequently sample stream a focus on after cross section
Shape is approximately the rectangle of 17 μ m 14 μm (wide × high), and the flow velocity of sample stream a reaches 5.1m/s.
Experiment can obtain, and this micro-channel structure 100 can be realized sample by regulation sample stream a flow and sheath liquid flow b flow
The regulation of sectional dimension after this stream a focusing.
Embodiment two
In the present embodiment, the height of ground floor fluid channel and third layer fluid channel is equal, is focused on by sample stream a to meet
The requirement of vertical centre.The height of second layer fluid channel can be different from the height of ground floor and third layer fluid channel, keep second
Layer fluid channel height is constant, by increasing or reduce ground floor fluid channel and the height of third layer fluid channel simultaneously, can increase
Or reduce sheath liquid flow b flow in this two-layer fluid channel, thus strengthen or to weaken the longitudinal focusing effect to sample stream a (right
Ground is answered to strengthen the transverse focusing effect to sample stream a after weakening).
Specifically, in this embodiment, the height of second layer fluid channel is that 150 μm, ground floor and third layer fluid channel are optional not
Same height, other sizes are all identical with embodiment one.Choose sample stream a flow 1 μ L/s, sheath liquid flow b flow 140 μ
L/s, after focusing, the flow velocity of sample stream a reaches 6.1m/s, sample stream a and is positioned at the center of runner.Such as:
The height choosing ground floor fluid channel and third layer fluid channel is 100 μm, now, and the cross section after sample stream a focusing
Shape is approximately the rectangle of 6 μ m 30 μm (wide × high).
The height choosing ground floor fluid channel and third layer fluid channel is 150 μm, now, and the cross section after sample stream a focusing
Shape is approximately the rectangle of 14 μ m 14 μm (wide × high).
The height choosing ground floor fluid channel and third layer fluid channel is 200 μm, now, and the cross section after sample stream a focusing
Shape is approximately the rectangle of 22 μ m 9 μm (wide × high).
Experiment can obtain, by adjusting ground floor fluid channel and the height of third layer fluid channel simultaneously, thus it is possible to vary sample stream a's
Sectional dimension after two-dimension focusing, can be by selecting suitable ground floor fluid channel and the thickness of third layer fluid channel, it is thus achieved that
The two-dimension focusing effect of required sample stream a.
Embodiment three
As it is shown in fig. 7, in the present embodiment, choose sample stream a flow 1 μ L/s, sheath liquid flow b flow 120 μ L/s,
After focusing, the flow velocity of sample stream a reaches 5.1m/s, sample stream a and is positioned at the center of runner, chooses line flowing channel portion 121 (131)
The micro-channel structure that front end face is plane, folded angular surface or arcwall face 100, other sizes all with the fluid channel of embodiment one
Structure 100 equivalently-sized.
When the front end face in line flowing channel portion 121 (131) uses plane, the sheath in ground floor fluid channel and third layer fluid channel
Liquid flow b is limited to the longitudinal focusing effect of sample stream a, and the cross sectional shape after sample stream a focuses on is approximately 6 μ m 50 μ
The rectangle of m (wide × high).
When the front end face in line flowing channel portion 121 (131) uses folded angular surface, sheath liquid flow b is in the cross section of fluid channel of a segment distance
Can be formed four symmetrical, there is the eddy current of longitudinal compression effect, these eddy current further enhancing the longitudinal direction to sample stream a
Focusing effect, as the front end face in line flowing channel portion 121 (131) is set to angle be 120 ° of folded angular surfaces time, sample stream a
Cross sectional shape after focusing is approximately the square of 15 μ m 15 μm (wide × high).
When the front end face in line flowing channel portion 121 (131) uses arcwall face, sheath liquid flow b can produce eddy current, further equally
Strengthen the longitudinal focusing to sample stream a, as the cross sectional shape after sample stream a focuses on when selecting semicircle is approximately 17 μ m
The rectangle of 14 μm (wide × high).
Experiment can obtain, and this micro-channel structure 100 can be by the wheel profile of the front end face in regulation line flowing channel portion 121 (131)
Shape realizes the regulation of sectional dimension after sample stream a focusing, thus obtains the different longitudinal focusing effect to sample stream a.
In sum, the single channel sheath fluid that utilizes according to embodiments of the present invention realizes the micro-channel structure of two dimensional fluid dynamic focus
100, there is two-dimension focusing function, by choosing different structural parameters and regulation liquid flow, so that focusing effect
Controlled, and have that flow resistance is low concurrently, be applicable to the advantages such as wide flow rates, high speed detection demand can be met, furthermore,
This micro-channel structure 100 can utilize single channel sheath fluid that the entrance of two sheath liquid flows b is merged into a road by conduit etc. and connected
To a liquid flowing source c, thus realize two dimensional fluid dynamic focus.
1 to Fig. 9 specifically describes micro-fluid chip 1 according to a second aspect of the present invention below in conjunction with the accompanying drawings.
The micro-fluid chip 1 of embodiment according to a second aspect of the present invention, including utilizing single channel sheath fluid real according to above-described embodiment
The micro-channel structure 100 of existing two dimensional fluid dynamic focus.Owing to the single channel sheath fluid that utilizes according to embodiments of the present invention realizes two dimension
The micro-channel structure 100 of Hydrodynamic focus has above-mentioned technique effect, therefore, microfluid core according to embodiments of the present invention
Sheet 1 also has above-mentioned technique effect, and i.e. the simple in construction of this micro-fluid chip 1, easy to manufacture, may be used for detect cell,
The biological particles such as embryo, RNA, DNA, protein particulate, microorganism, virus, in the detection, micro-fluid chip 1 is permissible
Stably the sample stream comprising biological particle is carried out two-dimension focusing so that it is focus on the center of fluid channel, to improve inspection
Accuracy, sensitivity and the stability surveyed.
Wherein, include according to one embodiment of present invention first lamellar body the 210, second lamellar body the 220, the 3rd lamellar body 230, on
Lid 240 and lower cover 250.Specifically, the first lamellar body 210 is formed with sample stream runner 111 and two transverse focusing streams
Runner 112, the second folded top being located at the first lamellar body 210 of lamellar body 220, and it is formed with upper strata on the second lamellar body 220 longitudinally
Focused flow runner 120, the 3rd folded lower section being located at the first lamellar body 210 of lamellar body 230, and be formed down on the 3rd lamellar body 230
Layer longitudinal focusing stream runner 130, the folded top being located at the second lamellar body 220 of upper cover 240, lower cover 250 is folded is located at the 3rd lamellar body
At least one in the lower section of 230, upper cover 240 and lower cover 250 is provided with focused flow inlet 243 and sample inflow entrance 111a
The sample streamer entrance 241 connected and the delivery outlet 242 connected with fluid outlet 111b.
With reference to Fig. 8 and Fig. 9, this micro-fluid chip 1 mainly by first lamellar body the 210, second lamellar body the 220, the 3rd lamellar body 230,
Upper cover 240 and lower cover 250 form.Wherein, upper cover the 240, second lamellar body the 220, first lamellar body the 210, the 3rd lamellar body 230,
Lower cover 250 superposition the most from top to bottom is arranged, upper cover the 240, second lamellar body the 220, first lamellar body the 210, the 3rd lamellar body 230,
The corner of lower cover 250 is equipped with through hole 244, facilitates the location of micro-fluid chip 1 and fixing, it is also possible to by thermocompression bonding,
The means of different such as gluing, bolt fastening, laser bonding, atomistic binding realize assembling.Alternatively, micro-fluid chip 1 is permissible
The unlike materials such as glass, quartz, high molecular polymer, pottery, metal are used to process.
Specifically, as shown in Fig. 1 to Fig. 7 and Fig. 9, the first lamellar body 210 is formed sample stream runner 111 and is located at
The transverse focusing stream runner 112 of sample stream runner 111 left and right sides, the second lamellar body 220 and the 3rd lamellar body 230 are respectively provided at
Above and below first lamellar body 210, and being formed with upper strata longitudinal focusing stream runner 120 on the second lamellar body 220, upper strata is indulged
Two transverse focusing streams are laid respectively to left side camber line flow path portion 122 and the right side camber line flow path portion 123 of focused flow runner 120
The top of runner 112 and turning on two transverse focusing stream runners 112, the line flowing channel of upper strata longitudinal focusing stream runner 120
Portion 121 is positioned at the top of sample stream runner 111 and turns on sample stream runner 111, upper strata longitudinal focusing stream runner 120
Align with two transverse focusing stream runners 112 and sample stream runner 111 setting in the vertical direction in outside, the 3rd lamellar body
Lower floor's longitudinal focusing stream runner 130 it is formed with, left side camber line flow path portion 132 He of lower floor's longitudinal focusing stream runner 130 on 230
Right side camber line flow path portion 133 lay respectively at two transverse focusing stream runners 112 lower section and with two transverse focusing stream runners 112
Conducting, the line flowing channel portion 131 of lower floor's longitudinal focusing stream runner 130 be positioned at sample stream runner 111 lower section and with sample stream
Runner 111 turns on, and the outside of lower floor's longitudinal focusing stream runner 130 is in the vertical direction and two transverse focusing stream runners 112
And the alignment of sample stream runner 111 is arranged, alternatively, two transverse focusing stream runners 112, upper strata longitudinal focusing stream runners
120 and the left side camber line flow path portion 122 (132) of lower floor's longitudinal focusing stream runner 130 and right side camber line flow path portion 123 (133)
The arc that formation is oppositely arranged and lateral profile is identical respectively, the width of transverse focusing stream runner 112 is respectively greater than sample stream stream
The width in road 111, left side camber line flow path portion 122 (132) and the width of right side camber line flow path portion 123 (133).
Wherein, at least one in the upper cover 240 of micro-fluid chip 1 and lower cover 250 is provided with watch window 245, it is simple to light
Learn detection.In upper cover 240 and lower cover 250 one is provided with sample streamer entrance 241, delivery outlet 242 and focused flow and injects
Mouthfuls 243, wherein, the sample inflow entrance 111a conducting of sample streamer entrance 241 and the first lamellar body 210, delivery outlet 242 and
Fluid outlet 111b conducting on first lamellar body 210, focused flow inlet 243 and the focused flow entrance of micro-channel structure 100
112a turns on, and by the conduit outside micro-fluid chip 1, the focused flow inlet 243 of two sheath liquid flows b can be merged into one
Road is also connected to a liquid flowing source c, thus realizes single sheath fluid and focus on.
In use, the micro-channel structure 100 of first aspect present invention embodiment both can be as in micro-fluid chip 1
Individual functional module, it is also possible to individually designed is a micro-fluid chip 1 being exclusively used in two dimensional fluid dynamic focus, and that makes is micro-
Fluid chip 1 both can coordinate other equipment to form a set of open system platform as a kind of function element, it is possible to
To develop portable instrument or equipment based on this, or substitute the Hydrodynamic focus device in traditional instrument or equipment.
This micro-fluid chip 1 may be used for detecting cell, embryo, RNA, DNA, protein particulate, microorganism, virus etc.
Biological particle.In the detection, the sample stream a comprising biological particle stably can be focused on miniflow by this micro-fluid chip 1
The center in road, to improve accuracy, sensitivity and the stability of detection.
Other of micro-fluid chip 1 according to embodiments of the present invention constitute and operate for those of ordinary skills
It is all known, is not detailed herein.
In describing the invention, it is to be understood that term " " center ", " longitudinally ", " laterally ", " length ", " width ",
" thickness ", " on ", D score, "front", "rear", "left", "right", " vertically ", " level ", " top ", " end " " interior ",
" outward ", " clockwise ", " counterclockwise ", " axially ", " radially ", " circumferential " etc. instruction orientation or position relationship be based on
Orientation shown in the drawings or position relationship, be for only for ease of the description present invention and simplify description rather than instruction or hint institute
The device that refers to or element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to this
Bright restriction.
Additionally, term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can be expressed
Or implicitly include at least one this feature.In describing the invention, " multiple " are meant that at least two, such as two
Individual, three etc., unless otherwise expressly limited specifically.
In the present invention, unless otherwise clearly defined and limited, term " install ", " being connected ", " connection ", " fixing "
Should be interpreted broadly Deng term, connect for example, it may be fixing, it is also possible to be to removably connect, or integral；Can be
Be mechanically connected, it is also possible to be electrical connection or each other can communication；Can be to be joined directly together, it is also possible to by the indirect phase of intermediary
Even, can be connection or the interaction relationship of two elements of two element internals, unless otherwise clear and definite restriction.For
For those of ordinary skill in the art, above-mentioned term concrete meaning in the present invention can be understood as the case may be.
In the present invention, unless otherwise clearly defined and limited, fisrt feature second feature " on " or D score can
Being that the first and second features directly contact, or the first and second features are by intermediary mediate contact.And, the
One feature second feature " on ", " top " and " above " but fisrt feature directly over second feature or
Oblique upper, or it is merely representative of fisrt feature level height higher than second feature.Fisrt feature second feature " under ",
" lower section " and " below " can be fisrt feature immediately below second feature or obliquely downward, or it is special to be merely representative of first
Levy level height less than second feature.
In the description of this specification, reference term " embodiment ", " some embodiments ", " example ", " specifically show
Example " or the description of " some examples " etc. means to combine this embodiment or example describes specific features, structure, material or
Feature is contained at least one embodiment or the example of the present invention.In this manual, the schematic representation to above-mentioned term
Necessarily it is directed to identical embodiment or example.And, the specific features of description, structure, material or feature are permissible
One or more embodiment in office or example combine in an appropriate manner.Additionally, in the case of the most conflicting, ability
The feature of the different embodiments described in this specification or example and different embodiment or example can be entered by the technical staff in territory
Row combines and combination.
Although above it has been shown and described that embodiments of the invention, it is to be understood that above-described embodiment is exemplary,
Being not considered as limiting the invention, those of ordinary skill in the art within the scope of the invention can be to above-described embodiment
It is changed, revises, replaces and modification.
Claims (14)
1. one kind utilizes the micro-channel structure that single channel sheath fluid realizes two dimensional fluid dynamic focus, it is characterised in that including:
Sample stream runner, described sample stream runner linearly extends at fore-and-aft direction, and the two ends of described sample stream runner form sample
This inflow entrance and fluid outlet；
Upper strata longitudinal focusing stream runner, described upper strata longitudinal focusing stream runner fold be located at described sample stream runner top and with institute
State sample stream flow passage；
Lower floor's longitudinal focusing stream runner, described lower floor longitudinal focusing stream runner fold be located at described sample stream runner lower section and with institute
State sample stream flow passage；
Two transverse focusing stream runners, said two transverse focusing stream runner is respectively provided at left side and the right side of described sample stream runner
Side, and respectively with described sample stream runner, described upper strata longitudinal focusing stream runner and described lower floor longitudinal focusing stream flow passage,
Each described transverse focusing stream runner on liquid flow direction, be gradually reduced from rear to front width, each described transverse focusing stream stream
The Breadth Maximum in road is more than the width of described sample stream runner.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 1, its feature
Being, said two transverse focusing stream runner is symmetrical about the longitudinal center cross section of described sample stream runner.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 1, its feature
Be, described upper strata longitudinal focusing stream runner and described lower floor longitudinal focusing stream runner about described sample stream runner horizontal in
Heart cross section is symmetrical above and below.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 1, its feature
Being, described upper strata longitudinal focusing stream runner and/or described lower floor longitudinal focusing stream runner include:
Line flowing channel portion, described line flowing channel portion and described sample stream runner alignment in the vertical direction；
Left side camber line flow path portion, described left side camber line flow path portion is located at the left side in described line flowing channel portion, and described left side camber line
The front end of flow path portion is tangent with the rear end in described line flowing channel portion and connects；
Right side camber line flow path portion, described right side camber line flow path portion is located at the right side in described line flowing channel portion, and described right side camber line
The front end of flow path portion is tangent with the rear end in described line flowing channel portion and connects,
The outboard wheel profile of described upper strata longitudinal focusing stream runner and described lower floor longitudinal focusing stream runner laterally gathers with said two
The outboard wheel profile alignment in the vertical direction that burnt stream runner is opposing.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 4, its feature
Being, the front end of described left side camber line flow path portion connects with the front end of described right side camber line flow path portion, and described left side camber line stream
Angle between the nearside wheel profile of road portion and described right side camber line flow path portion intersection is α, 50 °≤α≤80 °.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 4, its feature
Being, the front end face in described line flowing channel portion forms the forwardly convex on-plane surface of plane or middle part.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 4, its feature
Being, said two transverse focusing stream runner forms the arc of opposing setting respectively, and each described transverse focusing stream runner
Width is more than described left side camber line flow path portion and the width of described right side camber line flow path portion.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 7, its feature
Be, the opposing outboard wheel profile of two described transverse focusing stream runners and relative nearside wheel profile respectively with described sample
The tangent setting of outer contour of stream runner.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 1, its feature
Being, the longitudinal thickness of the longitudinal thickness of described sample stream runner and two described transverse focusing stream runners is equal, described upper strata
The longitudinal thickness of longitudinal focusing stream runner and described lower floor longitudinal focusing stream runner is equal.
The micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus the most according to claim 9, it is special
Levy and be, the longitudinal thickness of described sample stream runner and described upper strata longitudinal focusing stream runner/described lower floor longitudinal focusing stream runner
Longitudinal thickness equal.
11. micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus according to claim 9, it is special
Levy and be, the longitudinal thickness of described sample stream runner and described upper strata longitudinal focusing stream runner/described lower floor longitudinal focusing stream runner
Longitudinal thickness unequal.
12. micro-channel structure utilizing single channel sheath fluid to realize two dimensional fluid dynamic focus according to claim 1, it is special
Levy and be, also include two focused flow entrances, said two focused flow entrance respectively with two described transverse focusing stream runners,
Described upper strata longitudinal focusing stream runner and described lower floor longitudinal focusing stream flow passage, said two focused flow entrance passes through pipeline
Connect with a liquid flowing source.
13. 1 kinds of micro-fluid chips, it is characterised in that include according to utilizing single channel according to any one of claim 1-12
Sheath fluid realizes the micro-channel structure of two dimensional fluid dynamic focus.
14. micro-fluid chips according to claim 13, it is characterised in that including:
First lamellar body, described first lamellar body is formed described sample stream runner and two described transverse focusing stream runners；
Second lamellar body, described second lamellar body fold be formed on the top being located at described first lamellar body, and described second lamellar body described
Upper strata longitudinal focusing stream runner；
3rd lamellar body, described 3rd lamellar body fold be formed on the lower section being located at described first lamellar body, and described 3rd lamellar body described
Lower floor's longitudinal focusing stream runner；
Upper cover, described upper cover folds the top being located at described second lamellar body；
Lower cover, described lower cover fold in the lower section being located at described 3rd lamellar body, described upper cover and described lower cover at least one be provided with
Sample streamer entrance that focused flow inlet connects with described sample inflow entrance and the delivery outlet connected with described fluid outlet.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610210300.1A CN105854963B (en) | 2016-04-06 | 2016-04-06 | The micro-channel structure and micro-fluid chip of two dimensional fluid dynamic focus are realized using single channel sheath fluid |
| PCT/CN2016/105081 WO2017173820A1 (en) | 2016-04-06 | 2016-11-08 | Microchannel structure utilizing single-path sheath fluid for implementing two-dimensional hydrodynamic focusing and microfluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610210300.1A CN105854963B (en) | 2016-04-06 | 2016-04-06 | The micro-channel structure and micro-fluid chip of two dimensional fluid dynamic focus are realized using single channel sheath fluid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105854963A true CN105854963A (en) | 2016-08-17 |
| CN105854963B CN105854963B (en) | 2017-11-21 |
Family
ID=56628262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610210300.1A Active CN105854963B (en) | 2016-04-06 | 2016-04-06 | The micro-channel structure and micro-fluid chip of two dimensional fluid dynamic focus are realized using single channel sheath fluid |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN105854963B (en) |
| WO (1) | WO2017173820A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017173820A1 (en) * | 2016-04-06 | 2017-10-12 | 清华大学 | Microchannel structure utilizing single-path sheath fluid for implementing two-dimensional hydrodynamic focusing and microfluidic chip |
| CN113814008A (en) * | 2020-08-28 | 2021-12-21 | 上海交通大学 | Microfluidic channel structure, chip, particle ordered arrangement method and application |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111735854A (en) * | 2020-06-18 | 2020-10-02 | 东南大学 | Multi-mode accurately-focused electrical impedance flow cytometry detection device and preparation method thereof |
| CN115041243B (en) * | 2022-05-19 | 2023-11-10 | 珠海大略科技有限公司 | Micro-fluidic device for particle sorting and high concentration based on micropores |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1886315A (en) * | 2003-10-30 | 2006-12-27 | 赛托诺姆公司 | Multilayer hydrodynamic sheath flow structure |
| US8563325B1 (en) * | 2009-09-29 | 2013-10-22 | Sandia Corporation | Coaxial microreactor for particle synthesis |
| US20140261721A1 (en) * | 2008-01-16 | 2014-09-18 | Life Technologies Corporation | System and Method for Acoustic Focusing Hardware and Implementations |
| WO2014153107A1 (en) * | 2013-03-14 | 2014-09-25 | Cytonome/St, Llc | Hydrodynamic focusing apparatus and methods |
| CN105149019A (en) * | 2015-06-29 | 2015-12-16 | 清华大学 | Micro-channel structure used for two-dimensional hydrodynamic focusing and microfluid chip |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105854963B (en) * | 2016-04-06 | 2017-11-21 | 清华大学 | The micro-channel structure and micro-fluid chip of two dimensional fluid dynamic focus are realized using single channel sheath fluid |
-
2016
- 2016-04-06 CN CN201610210300.1A patent/CN105854963B/en active Active
- 2016-11-08 WO PCT/CN2016/105081 patent/WO2017173820A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1886315A (en) * | 2003-10-30 | 2006-12-27 | 赛托诺姆公司 | Multilayer hydrodynamic sheath flow structure |
| US20140261721A1 (en) * | 2008-01-16 | 2014-09-18 | Life Technologies Corporation | System and Method for Acoustic Focusing Hardware and Implementations |
| US8563325B1 (en) * | 2009-09-29 | 2013-10-22 | Sandia Corporation | Coaxial microreactor for particle synthesis |
| WO2014153107A1 (en) * | 2013-03-14 | 2014-09-25 | Cytonome/St, Llc | Hydrodynamic focusing apparatus and methods |
| CN105149019A (en) * | 2015-06-29 | 2015-12-16 | 清华大学 | Micro-channel structure used for two-dimensional hydrodynamic focusing and microfluid chip |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017173820A1 (en) * | 2016-04-06 | 2017-10-12 | 清华大学 | Microchannel structure utilizing single-path sheath fluid for implementing two-dimensional hydrodynamic focusing and microfluidic chip |
| CN113814008A (en) * | 2020-08-28 | 2021-12-21 | 上海交通大学 | Microfluidic channel structure, chip, particle ordered arrangement method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017173820A1 (en) | 2017-10-12 |
| CN105854963B (en) | 2017-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11873173B2 (en) | Multilayer hydrodynamic sheath flow structure | |
| US12044612B2 (en) | Method and apparatus for sorting particles using an array of asymmetrical obstacles | |
| Lenshof et al. | Continuous separation of cells and particles in microfluidic systems | |
| CN105854963A (en) | Micro-channel structure and micro-fluid chip for achieving two-dimensional fluid power accumulation by using single-way sheath liquid | |
| Sajeesh et al. | Particle separation and sorting in microfluidic devices: a review | |
| US20140227777A1 (en) | Cell sorting by 3d flow and adhesive rolling | |
| US9662602B2 (en) | Method of sorting particles or particle clusters in a fluid flowing in a channel | |
| Pamme | Continuous flow separations in microfluidic devices | |
| EP2040843B1 (en) | Apparatus for continuous particle separation | |
| Choi et al. | Hydrophoretic sorting of micrometer and submicrometer particles using anisotropic microfluidic obstacles | |
| Lubbersen et al. | Suspension separation with deterministic ratchets at moderate Reynolds numbers | |
| US20060290745A1 (en) | Method and apparatus for separating particles by dielectrophoresis | |
| CN209287355U (en) | Micro-fluidic chip and device containing the micro-fluidic chip | |
| Shen et al. | Ultra-low aspect ratio spiral microchannel with ordered micro-bars for flow-rate insensitive blood plasma extraction | |
| US20200269242A1 (en) | Device for separating or aligning fine particles, and method for separating or aligning fine particles using same | |
| CN108380254A (en) | Microfluidic chip liquid drop generating means | |
| CN105498875A (en) | Centrifugal micro-fluidic chip for preparing liquid drops | |
| CN102876563A (en) | Micro- fluid control chip capable of automatically catching single cells | |
| CN105772116A (en) | System for focusing or separating micro-nano particles and cells on basis of non-Newton effect, and method thereof | |
| CN105149019B (en) | Micro-channel structure used for two-dimensional hydrodynamic focusing and microfluid chip | |
| CN102836586B (en) | The method of hydrodynamic filter unit, fluid dynamic filter and Filtration Goal material | |
| CN106378217B (en) | The micro-fluidic chip of profit oil and two kinds of drops of water profit can be generated | |
| Loutherback | Microfluidic Devices for High Throughput Cell Sorting and Chemical Treatment | |
| US20040147043A1 (en) | Microfluidic system for the manipulation and concentration of particles suspended in liquid | |
| Bhagat et al. | Spiral microfluidic nanoparticle separators |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |