CN105854963B - The micro-channel structure and micro-fluid chip of two dimensional fluid dynamic focus are realized using single channel sheath fluid - Google Patents
The micro-channel structure and micro-fluid chip of two dimensional fluid dynamic focus are realized using single channel sheath fluid Download PDFInfo
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Abstract
Realize the micro-channel structure of two dimensional fluid dynamic focus the invention discloses a kind of using single channel sheath fluid and there is its micro-fluid chip, wherein micro-channel structure includes：Sample flow runner；Upper strata longitudinal focusing stream runner, the folded top for being located at sample flow runner of upper strata longitudinal focusing stream runner；Lower floor's longitudinal focusing stream runner, the folded lower section for being located at sample flow runner of lower floor's longitudinal focusing stream runner；Two transverse focusing stream runners, two transverse focusing stream runners are respectively provided at the left side and right side of sample flow runner, each transverse focusing stream runner is gradually reduced in liquid flow path direction, from rear to preceding width, and the Breadth Maximum of each transverse focusing stream runner is more than the width of sample flow runner.The micro-channel structure that two dimensional fluid dynamic focus is realized using single channel sheath fluid according to embodiments of the present invention, is realized the two-dimension focusing of sample flow, can prevent sample flow from being broken up by sheath liquid stream, is kept liquid stream stable, is ensured the two-dimension focusing effect of sample flow.
Description
Technical field
The present invention relates to microfluidic channel technical field, and two-dimensional flow is realized using single channel sheath fluid more particularly, to one kind
The micro-channel structure of body dynamic focus and be micro-fluid chip.
Background technology
With reference to flow cytometry, micro-fluid chip is commonly used for detecting cell, embryo, RNA, DNA, protein particulate, micro-
The biological particles such as biology, virus.In detection, micro-fluid chip needs stably to focus on the sample flow comprising biological particle micro-
The center of runner, to improve accuracy, sensitivity and the stability of detection.Process technology is limited to, fluid channel can be only capable of
Planar realize complexity curvilinear structures, but vertical direction be mostly vertical tube wall or the inclination determined by processing technology or
Bowl-type tube wall.Therefore, the micro-channel structure in correlation technique can complete the transverse focusing to sample flow in the horizontal plane, but difficult
With the two-dimension focusing completed the longitudinal focusing of vertical direction and include both direction simultaneously, both it is difficult to focus on sample flow
The center of fluid channel.Although some design structures can realize two-dimension focusing, section after flow velocity is relatively low, sample flow focuses on
Product is larger, and if center flow velocity less than 1m/s, the sectional area length of side is some tens of pm, and the center flow velocity of business flow cytometer is mostly
3-10m/s, the yardstick of biological particle are how below 10 μm or even smaller.Thus, these designs can not be realized to biological particle
High speed detection, while the degree of accuracy and the stability of detection are reduced, in addition, some micro-channel structures can realize two-dimension focusing,
And possesses the characteristics of high flow rate, small bore.
The content of the invention
It is contemplated that at least solves one of technical problem in correlation technique to a certain extent.Therefore, the present invention carries
Go out a kind of micro-channel structure that two dimensional fluid dynamic focus is realized using single channel sheath fluid, the canaliculus flow structure can utilize single channel sheath
Liquid realizes the function of two-dimension focusing, simple in construction.
The present invention also proposes a kind of there is the above-mentioned micro-channel structure that two dimensional fluid dynamic focus is realized using single channel sheath fluid
Micro-fluid chip.
The fluid channel knot that two dimensional fluid dynamic focus is realized using single channel sheath fluid of embodiment according to a first aspect of the present invention
Structure, including：Sample flow runner, the sample flow runner is in fore-and-aft direction along linear extension, the both ends shape of the sample flow runner
Into sample inflow entrance and fluid outlet；Upper strata longitudinal focusing stream runner, the upper strata longitudinal focusing stream runner is folded to be located at the sample
The top of this stream runner and with the sample flow flow passage；Lower floor's longitudinal focusing stream runner, lower floor's longitudinal focusing stream stream
The folded lower section for being located at the sample flow runner in road and with the sample flow flow passage；Two transverse focusing stream runners, described two
Individual transverse focusing stream runner is respectively provided at the left side and right side of the sample flow runner, and respectively with the sample flow runner, institute
Upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream flow passage are stated, each transverse focusing stream runner is in liquid stream
It is gradually reduced on direction, from rear to preceding width, the Breadth Maximum of each transverse focusing stream runner is more than the sample flow stream
The width in road.
The micro-channel structure that two dimensional fluid dynamic focus is realized using single channel sheath fluid according to embodiments of the present invention, by two
Transverse focusing stream runner is located at the left and right sides of sample focused flow runner, sample flow is realized transverse focusing first, that is, exists
The size of compression samples stream on horizontal direction, upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream runner are respectively provided at pair
Answer above and below the transverse focusing stream runner on position, sample flow is realized longitudinal focusing, i.e., when upper strata is longitudinally poly-
Sheath liquid stream in Jiao's stream runner and lower floor's longitudinal focusing stream runner symmetrically enters the compressible sample of sample flow runner in intermediate layer
The longitudinal size of stream, so as to realize the two-dimension focusing of sample flow on the whole, because the flow of sheath liquid stream is much larger than sample flow
Flow, the width of transverse focusing stream runner is set greater than to the width of sample flow runner, it can be ensured that sample flow and sheath liquid stream
When crossing, speed/dynamic pressure of sample flow is slightly larger than sheath liquid stream, prevents sample flow from being broken up by sheath liquid stream, and holding liquid stream is stable, ensures
The two-dimension focusing effect of sample flow.
In addition, the micro-channel structure that two dimensional fluid dynamic focus is realized using single channel sheath fluid according to embodiments of the present invention,
There can also be technical characteristic additional as follows：
According to one embodiment of present invention, longitudinal direction of described two transverse focusing stream runners on the sample flow runner
Central cross-section is symmetrical.
According to one embodiment of present invention, it is characterised in that the upper strata longitudinal focusing stream runner and the lower floor indulge
It is symmetrical above and below on the transverse center section of the sample flow runner to focused flow runner.
According to one embodiment of present invention, the upper strata longitudinal focusing stream runner and/or lower floor's longitudinal focusing stream
Runner includes：Line flowing channel portion, the line flowing channel portion and the sample flow runner alignment in the vertical direction；Left side arc
Line flow path portion, the left side camber line flow path portion are located at the left side in the line flowing channel portion, and before the left side camber line flow path portion
End is tangent with the rear end in the line flowing channel portion and connects；Right side camber line flow path portion, the right side camber line flow path portion are located at described
The right side in line flowing channel portion, and the front end of the right side camber line flow path portion is tangent with the rear end in the line flowing channel portion and connects,
The outboard wheel profile of the upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream runner and described two transverse focusings
Flow the opposite outboard wheel profile of runner alignment in the vertical direction.
According to one embodiment of present invention, the front end of the left side camber line flow path portion and the right side camber line flow path portion
Front end connects, and the angle between the inner side contour line of the left side camber line flow path portion and the right side camber line flow path portion intersection
For α, 50 °≤α≤80 °.
According to one embodiment of present invention, the front end face in the line flowing channel portion forms plane or middle part is forwardly convex
On-plane surface.
According to one embodiment of present invention, described two transverse focusing stream runners form the arc being disposed opposite to each other respectively,
And the width of each transverse focusing stream runner is more than the width of the left side camber line flow path portion and the right side camber line flow path portion
Degree.
According to one embodiment of present invention, the opposite outboard wheel profile of two transverse focusing stream runners and relative
The tangent setting of outer contour with the sample flow runner respectively of inner side contour line.
According to one embodiment of present invention, the longitudinal thickness of the sample flow runner and two transverse focusing stream streams
The longitudinal thickness in road is equal, the longitudinal thickness phase of the upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream runner
Deng.
According to one embodiment of present invention, the longitudinal thickness of the sample flow runner and the upper strata longitudinal focusing stream stream
The longitudinal thickness of road/lower floor longitudinal focusing stream runner is equal.
According to one embodiment of present invention, the longitudinal thickness of the sample flow runner and the upper strata longitudinal focusing stream stream
The longitudinal thickness of road/lower floor longitudinal focusing stream runner is unequal.
According to one embodiment of present invention, in addition to two focusing inflow entrances, it is described two focusing inflow entrances respectively with
Two transverse focusing stream runners, the upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream flow passage, institute
Two focusing inflow entrances are stated to connect with a liquid flowing source by pipeline.
The micro-fluid chip of embodiment according to a second aspect of the present invention, including the utilization single channel according to above-described embodiment
Sheath fluid realizes the micro-channel structure of two dimensional fluid dynamic focus.
According to one embodiment of present invention, including：First lamellar body, formed with the sample flow stream on first lamellar body
Road and two transverse focusing stream runners；Second lamellar body, the folded top for being located at first lamellar body of second lamellar body, and institute
State on the second lamellar body formed with the upper strata longitudinal focusing stream runner；3rd lamellar body, the 3rd lamellar body is folded to be located at described first
The lower section of lamellar body, and formed with lower floor's longitudinal focusing stream runner on the 3rd lamellar body；Upper lid, the upper lid is folded to be located at institute
State the top of the second lamellar body；Lower cover, the folded lower section for being located at the 3rd lamellar body of the lower cover, in the upper lid and the lower cover
It is at least one to connect provided with focused flow inlet, the sample flow inlet connected with the sample inflow entrance and with the fluid outlet
Logical delivery outlet.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Brief description of the drawings
Fig. 1 is the structural representation of micro-channel structure according to an embodiment of the invention；
Fig. 2 is the explosive view of the micro-channel structure shown in Fig. 1；
Fig. 3 is the structural representation of micro-channel structure according to embodiments of the present invention and the cross section of diverse location
Figure；
Fig. 4 is the structural representation according to the micro-channel structure of another embodiment of the present invention；
Fig. 5 is the top view of the micro-channel structure shown in Fig. 1；
Fig. 6 is the structural representation and partial enlarged drawing of the micro-channel structure shown in Fig. 1；
Fig. 7 is the structural representation and cross-sectional view of the micro-channel structure shown in Fig. 1；
The stereogram of micro-fluid chip according to embodiments of the present invention Fig. 8；
Fig. 9 is the explosive view of the structure shown in Fig. 8.
Reference：
a:Sample flow；b：Sheath liquid stream；c：Liquid flowing source；
1：Micro-fluid chip；
100：Micro-channel structure；
111：Sample flow runner；111a：Sample inflow entrance；111b：Fluid outlet；
112：Transverse focusing stream runner；112a：Focus on inflow entrance；
120：Upper strata longitudinal focusing stream runner；
121(131)：Line flowing channel portion；
122(132)：Left side camber line flow path portion；
123(133)：Right side camber line flow path portion；
130：Lower floor's longitudinal focusing stream runner；
210：First lamellar body；220：Second lamellar body；230：3rd lamellar body；240：Upper lid；241：Sample flow inlet；
242：Delivery outlet；243：Focused flow inlet；244：Through hole；245：Watch window；250：Lower cover.
Embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings.Below with reference to
The embodiment of accompanying drawing description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.
1 to Fig. 7 utilization single channel sheath fluid for specifically describing embodiment according to a first aspect of the present invention is realized below in conjunction with the accompanying drawings
The micro-channel structure 100 of two dimensional fluid dynamic focus.
As shown in figure 1, the fluid channel that two dimensional fluid dynamic focus is realized using single channel sheath fluid according to embodiments of the present invention
Structure 100 is indulged including 111, two transverse focusing stream runners 112 of sample flow runner, upper strata longitudinal focusing stream runner 120 and lower floor
To focused flow runner 130.Specifically, sample flow runner 111 in fore-and-aft direction along linear extension, the two of sample flow runner 111
End forms sample inflow entrance 111a and fluid outlet 111b, and upper strata longitudinal focusing stream runner 120 is folded to be located at sample flow runner 111
Top and connected with sample flow runner 111, the folded lower section for being located at sample flow runner 111 of lower floor's longitudinal focusing stream runner 130 and with
Sample flow runner 111 connects, and two transverse focusing stream runners 112 are respectively provided at the left side and right side of sample flow runner 111, and divide
Do not connected with sample flow runner 111, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130, it is each laterally poly-
Jiao's stream runner 112 is gradually reduced in liquid flow path direction, from rear to preceding width, the Breadth Maximum of each transverse focusing stream runner 112
More than the width of sample flow runner 111.
Alternatively, 111, two transverse focusing stream runners 112 of the sample flow runner of the micro-channel structure 100, upper strata longitudinal direction
Focused flow runner 120 and lower floor's longitudinal focusing stream runner 130 can be limited by the housing of micro-channel structure 100, it is preferable that sample
This stream runner 111 is formed extends linear, and cross sectional shape of the sample flow runner 111 on diverse location along the longitudinal direction
It is identical, area of section is equal, wherein, the rear end of sample flow runner 111 forms sample inflow entrance 111a, sample flow runner 111
Front end forms fluid outlet 111b, and sample flow runner 111, which can be used for circulating, sample flow a and focuses on liquid (such as sheath liquid stream b).
Two transverse focusing stream runners 112 are respectively provided at the left and right sides of sample flow runner 111 and two transverse focusing streams
Runner 112 connects with sample flow runner 111 respectively, and sample flow a enters in sample flow runner 111 from sample inflow entrance 111a, sheath
Liquid stream b enters the transverse focusing stream runner 112 of the left and right sides, the and of upper strata longitudinal focusing stream runner 120 from inflow entrance 112a is focused on
Lower floor's longitudinal focusing stream runner 130, sample flow a is first and the transverse focusing stream runner positioned at the left and right sides of sample flow runner 111
Sheath liquid stream b contacts in 112, as transverse focusing stream runner 112 gradually narrows, sheath liquid stream b symmetrically extrudes sample flow
A, keep sample flow a to be in left and right center and reduce its lateral dimension, realize the transverse focusing (left and right to sample flow a
The focusing in direction).
Upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 are respectively provided at the upper of sample flow runner 111
Lower both sides, and upper strata longitudinal focusing stream runner 120 gathers positioned at the top of two transverse focusing stream runners 112 and with two transverse directions
Jiao's stream runner 112 connects, lower floor's longitudinal focusing stream runner 130 positioned at the lower section of two transverse focusing stream runners 112 and with two
Transverse focusing stream runner 112 connects, and the upper strata longitudinal focusing stream runner 120 above and below sample flow runner 111 is with
Sheath liquid stream b in layer longitudinal focusing stream runner 130 extrudes liquid stream (sample flow a and the sheath fluid positioned at middle part runner symmetrical above and belowly
Stream b), ensures that it is in upper and lower center, it is achieved thereby that the longitudinal focusing (focusing of above-below direction) to sample flow a.With
Afterwards, sample flow a enters the leading portion of sample flow runner 111 under sheath liquid stream b parcel, and is stably positioned in sample flow runner 111
Central cross-section position, be easy to carry out all kinds of Photoelectric Detections to sample flow a.
That is, the micro-channel structure 100 is three layers of flow passage structure, three laminar flow roads are interconnected and interface is not present,
Wherein, sample flow runner 111 and the transverse focusing stream runner 112 being located at left and right sides of sample flow runner 111 are located at middle level, with
Sheath liquid stream b in two transverse focusing stream runners 112 from rear to preceding flowing, the sheath fluid in two transverse focusing stream runners 112
Stream b can realize the transverse focusing to sample flow a, and upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 divide
Not Wei Yu the upper and lower, be incorporated to the sample flow runner 111 positioned at middle level in the end (front end) of two longitudinal focusing stream runners,
Thickness with two longitudinal focusing stream runners (upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130) subtracts
Small, the sheath liquid stream b in two longitudinal focusing stream runners is incorporated in sample flow runner 111, it is possible to achieve sample flow a longitudinal direction is gathered
It is burnt.
Thus, the micro-channel structure that two dimensional fluid dynamic focus is realized using single channel sheath fluid according to embodiments of the present invention
100, two transverse focusing stream runners 112 are located to the left and right sides of sample focused flow runner, sample flow a is realized first
Transverse focusing, i.e., the compression samples stream a size on horizontal direction (left and right directions as shown in Figure 1), by upper strata longitudinal focusing
Stream runner 120 and lower floor's longitudinal focusing stream runner 130 be respectively provided at transverse focusing stream runner 112 on correspondence position top and
Lower section, sample flow a is set to realize longitudinal focusing, i.e., when upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner
Sheath liquid stream b in 130 symmetrically enters the compressible sample flow a of sample flow runner 111 in intermediate layer longitudinal size, so as to whole
Sample flow a two-dimension focusing is realized on body, because sheath liquid stream b flow is much larger than sample flow a flow, by transverse focusing stream stream
The width in road 112 is set greater than the width of sample flow runner 111, it can be ensured that when sample flow a and sheath liquid stream b cross, sample
Stream a speed/dynamic pressure is slightly larger than sheath liquid stream b, prevents sample flow a from being broken up by sheath liquid stream b, keeps liquid stream stable, ensures sample flow a
Two-dimension focusing effect.
Preferably, according to one embodiment of present invention, two transverse focusing stream runners 112 are on sample flow runner 111
Longitudinal center section it is symmetrical.Upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 are on sample flow
The transverse center section of runner 111 is symmetrical above and below.
That is, the micro-channel structure 100 of the embodiment of the present invention is mainly made up of three layers of fluid channel, present on the whole
Left and right and symmetrical above and below, up/down perforation between three layers of fluid channel, (indulge on upper strata in the absence of interface, first layer fluid channel above
To focused flow runner 120) structure and underlying third layer fluid channel (lower floor's longitudinal focusing stream runner 130) structure phase
Together, the structure positioned at two transverse focusing stream runners 112 of centre is identical.During work, sample flow a enters from sample inflow entrance 111a
Enter in the vertical direction, positioned at centre sample flow runner 111 in, sheath liquid stream b from focus on inflow entrance 112a enter the left and right sides
Transverse focusing stream runner 112, in upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130, sample flow a exists
Transverse focusing and longitudinal focusing are realized successively in the presence of sheath liquid stream b, that is, realize two-dimension focusing.Thus, by by two transverse directions
Focused flow runner 112, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 are arranged symmetrically in sample flow stream
The left and right in road 111, upper and lower orientation, it can be ensured that in the sheath liquid stream b pressure and transverse focusing stream runner 112 of longitudinal focusing stream runner
Flow stream pressure synchronously change, maintain liquid stream stable, so as to ensure sample flow a two-dimension focusing effect.
Wherein, as shown in Fig. 2 according to one embodiment of present invention, upper strata longitudinal focusing stream runner 120 and/or lower floor
Longitudinal focusing stream runner 130 includes line flowing channel portion 121 (131), left side camber line flow path portion 122 (132) and right side camber line runner
Portion 123 (133).Specifically, line flowing channel portion 121 (131) and sample flow runner 111 alignment in the vertical direction, it is left
Side camber line flow path portion 122 (132) is located at the left side in line flowing channel portion 121 (131), and before left side camber line flow path portion 122 (132)
End is tangent with the rear end in line flowing channel portion 121 and connects, and right side camber line flow path portion 123 (133) is located at line flowing channel portion 121
(131) right side, and the rear end in the front end of right side camber line flow path portion 123 (133) and line flowing channel portion 121 (131) is tangent and connects
It is logical, the outboard wheel profile and two transverse focusing stream streams of upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
The opposite outboard wheel profile in road 112 alignment in the vertical direction.
In other words, upper strata longitudinal focusing stream runner 120 is mainly by the line flowing channel portion above sample flow runner 111
121 and the left side camber line flow path portion 122 above two transverse focusing stream runners 112 and right side camber line flow path portion 123 form,
Lower floor's longitudinal focusing stream runner 130 is mainly by positioned at the line flowing channel portion 131 of the lower section of sample flow runner 111 and positioned at two transverse directions
The left side camber line flow path portion 132 and right side camber line flow path portion 133 of the lower section of focused flow runner 112 form.Wherein, a left side above
Side camber line flow path portion 122 and right side camber line flow path portion 123 are respectively with 121 tangent setting of line flowing channel portion above and connecting
And the position with two transverse focusing stream runners 112 corresponds respectively, underlying left side camber line flow path portion 132 and right side
Camber line flow path portion 133 respectively with underlying line flowing channel portion 131 it is tangent setting and connect and respectively with two transverse focusings
The position for flowing runner 112 corresponds.
During work, sample flow a from sample inflow entrance 111a enter sample flow runner 111 in, sheath liquid stream b from focus on inflow entrance
112a enters transverse focusing stream runner 112, upper strata longitudinal focusing stream runner 120 and the lower floor's longitudinal focusing stream runner of the left and right sides
In 130, first, sample flow a and the sheath liquid stream b in the transverse focusing stream runner 112 of the left and right sides of sample flow runner 111 connect
Touch, as transverse focusing stream runner 112 gradually narrows, sheath liquid stream b symmetrically extrudes sample flow a, maintains it in left and right
Center simultaneously reduces its lateral dimension, realizes the transverse focusing to sample flow a.Then, on sample flow runner 111
Sheath liquid stream b in the upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 of side and lower section is squeezed symmetrical above and belowly
Positioned at the liquid stream of middle part runner, (sample flow a and sheath liquid stream b), ensures that it is in upper and lower center, with line flowing channel portion to pressure
The reduction of 121 (131) longitudinal sizes, realizes the longitudinal focusing to sample flow a.Sample flow a is in horizontal and vertical both direction
On be focused, complete two-dimension focusing.
Then, sample flow a periphery enters the leading portion of sample flow runner 111, and stable status under sheath liquid stream b parcel
In the central cross-section position of sample flow runner 111, it is easy to carry out all kinds of Photoelectric Detections to sample flow a.In operation, into left and right two
The transverse focusing stream runner 112 of side and up and down the upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner of both sides
130 sheath liquid stream b is symmetrical, thus sheath liquid stream b can realize two-dimensional flow by a power source drive using single channel sheath fluid
Body dynamic focus, by choosing different structural parameters and regulation liquid flow, the micro-channel structure 100 of the embodiment of the present invention
Sample flow a can be focused to several microns to more than the 20 microns (feature in sample flow a sections under the center flow velocity of several metre per second (m/s)s
Size), effective focusing under high flow rate is realized, disclosure satisfy that high speed detection demand.
Thus, transverse focusing stream runner 112, upper strata longitudinal focusing stream runner 120 and the phase of lower floor's longitudinal focusing stream runner 130
Mutually conducting, and (upper strata is longitudinal with two longitudinal focusing stream runners by the opposite lateral profile of two transverse focusing stream runners 112
Focused flow runner 120 and lower floor's longitudinal focusing stream runner 130) opposite lateral profile be arranged to it is identical, it can be ensured that longitudinal direction is poly-
The sheath liquid stream b pressure change synchronous with the flow stream pressure in transverse focusing stream runner 112 of Jiao's stream runner, maintain liquid stream stable.
Alternatively, according to one embodiment of present invention, the front end of left side camber line flow path portion 122 (132) and right side camber line
The front end connection of flow path portion 123 (133), and left side camber line flow path portion 122 (132) crosses with right side camber line flow path portion 123 (133)
The angle located between A inner side contour line is α, 50 °≤α≤80 °.
Reference picture 5, the left side camber line flow path portion 122 and right side camber line flow path portion 123 of upper strata longitudinal focusing stream runner 120 from
Gradually cross to preceding afterwards and connect its front end and the rear end in the line flowing channel portion 121 of upper strata longitudinal focusing stream runner 120, lower floor
The left side camber line flow path portion 132 and right side camber line flow path portion 133 of longitudinal focusing stream runner 130 gradually cross from rear to preceding and make it
Front end connects with the line flowing channel portion 131 of lower floor longitudinal focusing stream runner 130.
Wherein, left side camber line flow path portion 122 (132) and (133) intersection A of right side camber line flow path portion 123 inner side profile
Angle between line is controlled between 50 ° to 80 °, such as 50 °, 60 ° or 80 °, it can so ensure left side camber line flow path portion
122 (132) and the intersection A of right side camber line flow path portion 123 (133) have certain length L, pass through experiment：With left in layer
Side camber line flow path portion 122 (132) and the intersection A of right side camber line flow path portion 123 (133) runner profile need to meet special want
Ask：Intersection A profiles need to keep suitable length L in fore-and-aft direction；If the length L of intersection's A profiles is long can increase on
Sheath liquid stream b in layer longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 to sample flow a transverse shear stress, weaken
Longitudinal direction extruding, so as to increase the height dimension in section after sample flow a is focused on；If the length L of intersection's A profiles is too short to cause a left side
Side camber line flow path portion 122 (132) can not be timely due to effect of inertia with the sheath liquid stream in right side camber line flow path portion 123 (133)
Cross, and local depression is produced in intersection A, while make second layer fluid channel (sample flow runner 111 and two transverse focusing streams
Runner 112) in liquid stream extended into upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130, can equally increase
The height dimension in the section after large sample stream a focusing.
Thus, by by left side camber line flow path portion 122 (132) and (133) intersection A profiles of right side camber line flow path portion 123
Wire clamp angle is controlled between 50 ° to 80 °, it is ensured that left side camber line flow path portion 122 and the right side above sample flow runner 111
Side camber line flow path portion 123, the left side camber line flow path portion 132 positioned at the lower section of sample flow runner 111 and right side camber line flow path portion 133 are handed over
A has suitable length L at remittance so that left side camber line flow path portion 122 (132) and the sheath fluid in right side camber line flow path portion 123 (133)
Stream b with the two cross, in the vertical direction, the sample flow a that is coated with sheath liquid stream b to the left and right sides extrude, to have
The stable two femoral sheath liquid stream b merged with sample flow a in effect ground, the longitudinal direction to sample flow a that contributing to lifting downstream will be carried out are gathered
Jiao, so as to ensure longitudinal focusing effects of the sheath liquid stream b to sample flow a.
Alternatively, as shown in B portions in Fig. 6, according to one embodiment of present invention, the front end face shape in line flowing channel portion 121
The on-plane surface forwardly convex into plane or middle part.During work, the upper strata longitudinal direction above two transverse focusing stream runners 112
Sheath liquid stream b in the left side camber line flow path portion 122 and right side camber line flow path portion 123 of focused flow runner 120 the two end that crosses,
Into the line flowing channel portion 121 above sample flow runner 111, the lower floor positioned at two lower sections of transverse focusing stream runner 112
Sheath liquid stream b in the left side camber line flow path portion 132 and right side camber line flow path portion 133 of longitudinal focusing stream runner 130 crosses at the two
End, into positioned at the line flowing channel portion 131 of the lower section of sample flow runner 111.
In some specific example examples of the present invention, by the front end face shape in the line flowing channel portion 121 (131) of upper and lower both sides
Into plane, the effect of longitudinal focusing can be played to sample flow a；In other specific examples of the present invention, by two straight lines
The front end face of flow path portion 121 (131) can form the forwardly convex on-plane surface in middle part, if such as line flowing channel portion 121 (131)
Front end face forms that middle part is forwardly convex and symmetrical folded angular surface, that is, forms two intersecting planes, such sheath liquid stream b one section away from
From cross section of fluid channel in can form four vortex symmetrical, that there is longitudinal compression effect, these vortex further enhancing pair
Sample flow a longitudinal focusing effect.
Preferably, as shown in fig. 7, by the front end face in the line flowing channel portion 121 of upper strata longitudinal focusing stream runner 120 and lower floor
The front end face in the line flowing channel portion 131 of longitudinal focusing stream runner 130 is respectively formed the forwardly convex arcwall face in middle part, such sheath liquid stream
B formed in the cross section of fluid channel of a segment distance four in above-below direction and left and right directions respectively symmetrically, there is longitudinal compression effect
The vortex of fruit, in the process, with the thickness of upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
Reduce, sheath liquid stream b compression samples streams a longitudinal size, so as to realize the longitudinal focusing to sample flow a, furthermore, sheath liquid stream b's
Four vortex can further enhance the longitudinal focusing to sample flow a, ensure the effect of sample flow a longitudinal focusing.Specifically, may be used
According to the desired size after different flows and sample flow a focusing, to choose different end profiles to realize.
Thus, can by the way that the front end face in line flowing channel portion 121 (131) is arranged into the forwardly convex on-plane surface in middle part
Sheath liquid stream b is set to form four vortex symmetrical, that there is longitudinal compression effect, these whirlpools in the section of sample flow runner 111
Stream can further enhance the longitudinal focusing effect to sample flow a.
Alternatively, according to one embodiment of present invention, two transverse focusing stream runners 112 form what is be disposed opposite to each other respectively
Arc, and the width of each transverse focusing stream runner 112 is more than left side camber line flow path portion 122 (132) and right side camber line flow path portion
The width of 123 (133).
In other words, sample flow runner 111 forms the straight channel extended along the longitudinal direction, two transverse focusing stream runners 112
It is located at the left and right sides of sample flow runner 111 and two transverse focusing stream runners 112 forms two arc fluid channels to cross, position
The left side camber line flow path portion 122 and right side camber line flow path portion of upper strata longitudinal focusing stream runner 120 above sample flow runner 111
123rd, the left side camber line flow path portion 132 and right side camber line of lower floor's longitudinal focusing stream runner 130 below sample flow runner 111
Flow path portion 133 forms arc fluid channel corresponding with the appearance profile of two transverse focusing stream runners 112 respectively.By transverse focusing
The width of stream runner 112 is set greater than the width of left side camber line flow path portion 132 and right side camber line flow path portion 133 so that two
Sheath liquid stream b in transverse focusing stream runner 112 gradually crosses in liquid flow path direction first, during work, positioned at sample flow runner 111
The left and right sides transverse focusing stream runner 112 in sheath liquid stream b contacted first with sample flow a, with transverse focusing stream runner
112 gradually narrow, and sheath liquid stream b symmetrically extrudes sample flow a, maintains it in left and right center and reduces its transverse direction
Size, realize the transverse focusing to sample flow a.Then upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner
Sheath liquid stream b in 130 extrudes sample flow a symmetrical above and belowly, realizes the longitudinal focusing to sample flow a.
Thus, two transverse focusing stream runners 112 are symmetrically arranged to the left and right sides of sample flow runner 111, by sample
Stream a focuses on center position, sheath liquid stream b is introduced in symmetrical transverse focusing stream runner 112, it is possible to achieve to sample
Flow a transverse focusing；Because sheath liquid stream b flow is much larger than sample flow a flow, by the width of transverse focusing stream runner 112
It is set greater than the width of sample flow runner 111, it can be ensured that when sample flow a and sheath liquid stream b cross, sample flow a speed/dynamic
Pressure is slightly larger than sheath liquid stream b, prevents sample flow a from being broken up by sheath liquid stream b.The width of transverse focusing stream runner 112 is set greater than
The width of left side camber line flow path portion 122 (132) or right side camber line flow path portion 123 (133), be advantageous to ensureing sample flow a first
Transverse focusing is carried out, until in the case that transverse focusing is stable, realizing longitudinal focusing to sample flow a again, improves the two of sample flow a
Tie up focusing effect.
Preferably, according to one embodiment of present invention, the opposite outboard wheel profile of two transverse focusing stream runners 112
With the tangent setting of outer contour with sample flow runner 111 respectively of relative inner side contour line.Thus, two transverse focusing stream stream
Road 112 with sample flow runner 111 is tangent, slowly integrates the sample flow runner 111 of centre in the longitudinal direction, can about bundle-sheath
Liquid stream b focus on and steering procedure in keep laminar condition, flow to smooth change, streamline without mutation, so as to reduce flow resistance,
Improve the stability of a system.The longer distance that crosses, can make sample between transverse focusing stream runner 112 and sample flow runner 111
Flow a before being crossed with the sheath liquid stream b in longitudinal focusing stream runner, reach stable transverse focusing state, ensure the stabilization of liquid stream
Property.
Wherein, according to one embodiment of present invention, the longitudinal thickness of sample flow runner 111 and two transverse focusing stream streams
The longitudinal thickness in road 112 is equal, the longitudinal thickness phase of upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
Deng.So as to ensure the stability of transverse focusing, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130 it is vertical
It is equal to thickness, so as to ensure the stability of longitudinal focusing.
In certain specific embodiments of the invention, longitudinal thickness and the upper strata longitudinal focusing stream of sample flow runner 111
The longitudinal thickness of the lower floor's longitudinal focusing stream runner 130 of runner 120/ is equal.In other words, positioned at two transverse focusing stream runners 112
Top upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing positioned at the lower section of two transverse focusing stream runners 112
It is equal to flow the thickness of runner 130 in the vertical direction, and the thickness of upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing
It is equal with the thickness of 111, two transverse focusing stream runners 112 of sample flow runner respectively to flow the thickness of runner 130, in fluid channel knot
Structure 100 on the whole, the thickness of three layers of fluid channel it is equal, the requirement that sample flow a is focused on to vertical centre can be met.
In other embodiments of the present invention, longitudinal thickness and the upper strata longitudinal focusing of sample flow runner 111
The longitudinal thickness for flowing the lower floor's longitudinal focusing stream runner 130 of runner 120/ is unequal.In other words, positioned at two transverse focusing stream runners
The upper strata longitudinal focusing stream runner 120 of 112 top is longitudinally poly- with the lower floor positioned at the lower section of two transverse focusing stream runners 112
The thickness of Jiao's stream runner 130 in the vertical direction is equal, and the thickness of upper strata longitudinal focusing stream runner 120 and lower floor are longitudinally poly-
The thickness of Jiao's stream runner 130 is unequal with the thickness of 111, two transverse focusing stream runners 112 of sample flow runner respectively, in miniflow
Road structure 100 on the whole, the thickness of fluid channel above and lower section and positioned at middle part fluid channel thickness it is unequal,
By adjusting the thickness of longitudinal focusing stream runner, flows of the sheath liquid stream b in longitudinal focusing stream runner can be increased or decreased, from
And strengthen or weaken the longitudinal focusing effect to sample flow a.
Wherein, according to one embodiment of present invention, in addition to two focus on inflow entrance 112a, two focusing inflow entrances
112a respectively with two transverse focusing stream runners 112, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
Connection, two focus on inflow entrance 112a and are connected by pipeline with a liquid flowing source c.
Specifically, as shown in figure 1, positioned at the rear end of the transverse focusing stream runner 112 in sample flow a left side, upper strata longitudinal direction
The rear end of 120 left side camber line flow path portion 122 and the rear end of the left side camber line flow path portion 132 of lower floor's longitudinal focusing stream runner 130
Formed one focusing inflow entrance 112a, i.e. the focusing inflow entrance 112a at the same with the transverse focusing stream runner 112 positioned at left side, on
The rear end conducting of layer longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130, the transverse direction on the right side of sample flow a are gathered
The rear end of Jiao's stream runner 112, the rear end of the right side camber line flow path portion 123 of upper strata longitudinal direction 120 and lower floor's longitudinal focusing stream runner 130
Right side camber line flow path portion 133 rear end formed another focus on inflow entrance 112a, i.e. the focusing inflow entrance 112a at the same with position
In the rear end of the transverse focusing stream runner 112 on right side, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
Conducting.
Wherein, sheath liquid stream b can by a power source drive by two focus on inflow entrance 112a enter it is symmetrical
In transverse focusing stream runner 112 and upper strata longitudinal focusing stream runner 120 symmetrical above and below and lower floor's longitudinal focusing stream runner 130,
Two dimensional fluid dynamic focus is realized using single channel sheath fluid, ensures that sheath liquid stream b's is symmetrical.The micro-channel structure 100 can
Under the center flow velocity of many metres per second by sample flow a focus to its section characteristic size it is several be micron to more than 20 microns, i.e.,
Effective focusing under high flow rate can be realized.
Utilization single channel sheath fluid according to embodiments of the present invention, which is specifically described, with reference to multiple embodiments realizes two dimensional fluid
The micro-channel structure 100 of dynamic focus.
Micro-channel structure 100 on the whole, present left and right and it is symmetrical above and below, it is made up of three layers of fluid channel, three layers of miniflow
Up/down perforation between road, in the absence of interface, wherein, second layer fluid channel be located at first layer fluid channel and third layer fluid channel it
Between, first layer is identical with the structure of third layer fluid channel, as shown in figure 3, first layer fluid channel is upper strata longitudinal focusing stream runner
120, longitudinal focusing stream runner 120 includes line flowing channel portion 121 and two left side camber line flow path portions 122 to cross at the middle and upper levels for it
With right side camber line flow path portion 123, third layer fluid channel is lower floor's longitudinal focusing stream runner 130, wherein lower floor's longitudinal focusing stream stream
Road 130 includes line flowing channel portion 131 and two left side camber line flow path portions 132 and right side camber line flow path portion 133 to cross, and second
Layer fluid channel is mainly by a sample flow runner 111 in an intermediate position and two cambers for importing sample flow runner 111
Transverse focusing stream runner 112 form, and the width of transverse focusing stream runner 112 be more than sample flow runner 111 width；Three
All arc fluid channels have identical lateral profile in layer fluid channel.
During work, sample flow a enters from the sample inflow entrance 111a of sample flow runner 111, and sheath liquid stream b is from focusing on inflow entrance
112a enters arc fluid channel (including the transverse focusing stream runner positioned at the left and right sides of sample flow runner 111 of the left and right sides
112nd, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130), sample flow a in the presence of sheath liquid stream b according to
It is secondary to realize transverse focusing and longitudinal focusing, i.e. two-dimension focusing.First, two arc fluid channels of sample flow a and the second layer are (horizontal
Focused flow runner 112) in sheath liquid stream b contact, as transverse focusing stream runner 112 gradually narrows, sheath liquid stream b is symmetrically
Extruding sample flow a, maintain it in left and right center and reduce its lateral dimension, realize and sample flow a transverse direction is gathered
Jiao, cross section of fluid channel as shown in Figure 3.
Then, in the end of transverse focusing stream runner 112 (front end as shown in Figure 3), first layer fluid channel (upper strata longitudinal direction
Focused flow runner 120) and third layer fluid channel (lower floor's longitudinal focusing stream runner 130) in sheath liquid stream b to enter the second layer micro-
In runner, the liquid stream in second layer fluid channel is extruded symmetrical above and belowly, is maintained it in center up and down and is reduced its longitudinal direction
Size, it is achieved thereby that the longitudinal focusing to sample flow a.Sample flow a is focused in horizontal and vertical both direction, is completed
Two-dimension focusing.Afterwards, sample flow a enters the leading portion of sample flow runner 111 under sheath liquid stream b parcel, and is stably positioned in sample
The center in the section of this stream runner 111, such as the cross section of fluid channel in Fig. 3, consequently facilitating carrying out all kinds of photoelectricity inspections to sample flow a
Survey.Preferably, as shown in figure 4, in operation, the sheath liquid stream b into left and right sides arc fluid channel is symmetrical, thus sheath fluid
Flowing b can be supplied by a liquid flowing source c, i.e., realize two dimensional fluid dynamic focus using single channel sheath fluid.
Embodiment one
First layer fluid channel and third layer fluid channel connect with the fluid channel of the second layer, and have same outside
Profile, to ensure sheath liquid stream b pressure therein change synchronous with the flow stream pressure in second layer fluid channel, and maintain liquid in system
The stabilization of stream；First layer fluid channel (upper strata longitudinal focusing stream runner 120) or third layer fluid channel (lower floor's longitudinal focusing stream runner
130) cross two arc fluid channels (left side camber line flow path portion 122 (132) and right side camber line flow path portion 123 (133)) position in
The runner profile at place need to need to keep suitable length L in fore-and-aft direction, and length is long to increase first layer fluid channel and third layer
The height in section after sheath liquid stream b in fluid channel focuses on to sample flow a transverse shear stress, the extruding of decrease longitudinal direction, increase sample flow a
Size, length is too short, and the sheath liquid stream b in arc fluid channel can not be crossed, in intersection A in time due to effect of inertia
The liquid stream produced in local depression, second layer fluid channel extends into first layer fluid channel and third layer fluid channel, increases sample
Flow the height dimension in section after a is focused on, therefore, the left side camber line flow path portion 122 of the micro-channel structure 100 of the embodiment of the present invention
(132) and the runner profile of the opening position that crosses of right side camber line flow path portion 123 (133) uses -80 ° of α=50 ° structure, so as to solve
Certainly above mentioned problem.
Specifically, in the present embodiment, the height of second layer fluid channel and first layer fluid channel and third layer fluid channel
Highly equal, the height of three layers of fluid channel is chosen for 150 μm, and the width positioned at the sample flow runner 111 of the second layer is chosen for
300 μm, profile angle is chosen on the inside of two arc fluid channel intersection A runner in first layer fluid channel or third layer fluid channel
For 70 °, the front end face of two line flowing channel portions 121 (131) above and below sample flow runner 111 is arcwall face, should
Micro-channel structure 100 can be realized to be focused in high speed, and sample flow a flow velocity is up to number metre per second (m/s) after focusing.
First, selection sample flow a flows, which are 0.5 μ L/s, sheath liquid stream b flows are that 120 μ L/s are two-dimentional to sample flow a progress gathers
Jiao's experiment, then, the cross sectional shape after sample flow a focusing are approximately square, the sample flow a stream of 12 μm of 12 μ m (wide × high)
Speed reaches 5.1m/s.
Choose sample flow a flows be 1 μ L/s, sheath liquid stream b flows be 100 μ L/s, subsequent sample flow a focus on after section shape
Shape is approximately the rectangle of 24 μm of 13 μ m (wide × high), and now, sample flow a is located at the center of runner, and sample flow a flow velocity reaches
4.3m/s。
Choose sample flow a flows be 1 μ L/s, sheath liquid stream b flows be 120 μ L/s, subsequent sample flow a focus on after section shape
Shape is approximately the rectangle of 14 μm of 17 μ m (wide × high), and sample flow a flow velocity reaches 5.1m/s.
Experiment can obtain, and the micro-channel structure 100 can be realized pair by adjusting sample flow a flows and sheath liquid stream b flows
The regulation of sectional dimension after sample flow a is focused on.
Embodiment two
In the present embodiment, the height of first layer fluid channel and third layer fluid channel is equal, to meet to focus on sample flow a
In the requirement of vertical centre.The height of second layer fluid channel can be different from the height of first layer and third layer fluid channel, keep the
Two layers of fluid channel are highly constant, by increasing or reducing the height of first layer fluid channel and third layer fluid channel, Ke Yizeng simultaneously
Add deduct flows of few sheath liquid stream b in this two layers of fluid channel, so as to strengthen or weaken the longitudinal focusing effect to sample flow a
(transverse focusing effect of the enhancing to sample flow a after accordingly weakening).
Specifically, the height of second layer fluid channel is that 150 μm, first layer and third layer fluid channel are optional not in the embodiment
Same height, other sizes are identical with embodiment one.μ L/s of sample flow a flows 1, the μ L/s of sheath liquid stream b flows 140 are chosen, is gathered
Defocused sample flow a flow velocity reaches 6.1m/s, and sample flow a is located at the center of runner.Such as：
The height for choosing first layer fluid channel and third layer fluid channel is 100 μm, now, the section after sample flow a focusing
Shape is approximately the rectangle of 30 μm of 6 μ m (wide × high).
The height for choosing first layer fluid channel and third layer fluid channel is 150 μm, now, the section after sample flow a focusing
Shape is approximately the rectangle of 14 μm of 14 μ m (wide × high).
The height for choosing first layer fluid channel and third layer fluid channel is 200 μm, now, the section after sample flow a focusing
Shape is approximately the rectangle of 9 μm of 22 μ m (wide × high).
Experiment can obtain, by the height for adjusting first layer fluid channel and third layer fluid channel simultaneously, thus it is possible to vary sample flow a
Two-dimension focusing after sectional dimension, can be obtained by selecting the thickness of suitable first layer fluid channel and third layer fluid channel
Obtain required sample flow a two-dimension focusing effect.
Embodiment three
As shown in fig. 7, in the present embodiment, μ L/s of sample flow a flows 1, the μ L/s of sheath liquid stream b flows 120 are chosen, after focusing
Sample flow a flow velocity reaches 5.1m/s, and sample flow a is located at the center of runner, chooses the front end face of line flowing channel portion 121 (131)
For the micro-channel structure 100 of plane, folded angular surface or arcwall face, other sizes with the micro-channel structure 100 of embodiment one
Size is identical.
When the front end face in line flowing channel portion 121 (131) uses plane, in first layer fluid channel and third layer fluid channel
Longitudinal focusing effects of the sheath liquid stream b to sample flow a is limited, and the cross sectional shape after sample flow a is focused on is approximately that 50 μm of 6 μ m is (wide
× it is high) rectangle.
When the front end face in line flowing channel portion 121 (131) uses folded angular surface, cross section of fluid channel of the sheath liquid stream b in a segment distance
Interior to form four vortex that are symmetrical, having longitudinal compression effect, these vortex further enhancing the longitudinal direction to sample flow a
Focusing effect, when such as to be arranged to angle be 120 ° of folded angular surfaces by the front end face in line flowing channel portion 121 (131), after sample flow a is focused on
Cross sectional shape be approximately the square of 15 μm of 15 μ m (wide × high).
When the front end face in line flowing channel portion 121 (131) uses arcwall face, sheath liquid stream b can equally produce vortex, further
Strengthen the longitudinal focusing to sample flow a, the cross sectional shape after sample flow a is focused on when such as selecting semicircle is approximately 14 μm of 17 μ m
The rectangle of (wide × high).
Experiment can obtain, and the micro-channel structure 100 can pass through the profile for the front end face for adjusting line flowing channel portion 121 (131)
Shape come realize sample flow a is focused on after sectional dimension regulation, so as to which the different longitudinal focusings obtained to sample flow a are imitated
Fruit.
In summary, the fluid channel knot that two dimensional fluid dynamic focus is realized using single channel sheath fluid according to embodiments of the present invention
Structure 100, there is two-dimension focusing function, by choosing different structural parameters and regulation liquid flow, can to focus on and imitate
Fruit is controllable, and has the advantages that flow resistance is low, suitable for wide flow rates concurrently, can meet high speed detection demand, furthermore, should
Two sheath liquid stream b entrance can be merged into all the way and be connected to by conduit etc. using single channel sheath fluid by micro-channel structure 100
One liquid flowing source c, so as to realize two dimensional fluid dynamic focus.
1 to Fig. 9 specifically describe micro-fluid chip 1 according to a second aspect of the present invention below in conjunction with the accompanying drawings.
The micro-fluid chip 1 of embodiment according to a second aspect of the present invention, including the utilization single channel sheath according to above-described embodiment
Liquid realizes the micro-channel structure 100 of two dimensional fluid dynamic focus.Because utilization single channel sheath fluid according to embodiments of the present invention is realized
The micro-channel structure 100 of two dimensional fluid dynamic focus has above-mentioned technique effect, therefore, microfluid according to embodiments of the present invention
Chip 1 also has above-mentioned technique effect, i.e. the structure of the micro-fluid chip 1 is simple, easy to manufacture, can be used for detect cell,
The biological particles such as embryo, RNA, DNA, protein particulate, microorganism, virus, in the detection, micro-fluid chip 1 can be stably
Sample flow comprising biological particle is subjected to two-dimension focusing, it is focused on the center of fluid channel, to improve the standard of detection
True property, sensitivity and stability.
Wherein, according to one embodiment of present invention including the first lamellar body 210, the second lamellar body 220, the 3rd lamellar body 230, on
Lid 240 and lower cover 250.Specifically, formed with sample flow runner 111 and two transverse focusing stream runners on the first lamellar body 210
112, the folded top for being located at the first lamellar body 210 of the second lamellar body 220, and formed with upper strata longitudinal focusing stream stream on the second lamellar body 220
Road 120, the folded lower section for being located at the first lamellar body 210 of the 3rd lamellar body 230, and formed with lower floor's longitudinal focusing stream on the 3rd lamellar body 230
Runner 130, the folded top for being located at the second lamellar body 220 of upper lid 240, the folded lower section for being located at the 3rd lamellar body 230 of lower cover 250, upper lid 240
With at least one sample flow inlet for being provided with focused flow inlet 243, being connected with sample inflow entrance 111a in lower cover 250
241 and the delivery outlet 242 that is connected with fluid outlet 111b.
Reference picture 8 and Fig. 9, the micro-fluid chip 1 mainly by the first lamellar body 210, the second lamellar body 220, the 3rd lamellar body 230,
Upper lid 240 and lower cover 250 form.Wherein, upper lid 240, the second lamellar body 220, the first lamellar body 210, the 3rd lamellar body 230, lower cover 250
Superposition is set from top to bottom successively, upper lid 240, the second lamellar body 220, the first lamellar body 210, the 3rd lamellar body 230, the four of lower cover 250
Angle is equipped with through hole 244, facilitates the positioning and fixation of micro-fluid chip 1, can also by thermocompression bonding, gluing, bolt fasten,
The means of different such as laser bonding, atomistic binding realize assembling.Alternatively, micro-fluid chip 1 can use glass, quartz, high score
The unlike materials such as sub- polymer, ceramics, metal are process.
Specifically, as shown in Fig. 1 to Fig. 7 and Fig. 9, formed with sample flow runner 111 and being located at sample on the first lamellar body 210
The transverse focusing stream runner 112 of the left and right sides of this stream runner 111, the second lamellar body 220 and the 3rd lamellar body 230 are respectively provided at first
Above and below body 210, and formed with upper strata longitudinal focusing stream runner 120, upper strata longitudinal focusing stream stream on the second lamellar body 220
The left side camber line flow path portion 122 and right side camber line flow path portion 123 in road 120 are respectively positioned at the upper of two transverse focusing stream runners 112
Turn on just and with two transverse focusing stream runners 112, the line flowing channel portion 121 of upper strata longitudinal focusing stream runner 120 is located at sample
Flow the top of runner 111 and turned on sample flow runner 111, the outside of upper strata longitudinal focusing stream runner 120 in the vertical direction,
Alignd with two transverse focusing stream runners 112 and sample flow runner 111 setting, formed with lower floor longitudinal direction on the 3rd lamellar body 230
Focused flow runner 130, the left side camber line flow path portion 132 and right side camber line flow path portion 133 of lower floor's longitudinal focusing stream runner 130 are distinguished
Turned on positioned at the lower section of two transverse focusing stream runners 112 and with two transverse focusing stream runners 112, lower floor's longitudinal focusing stream stream
The line flowing channel portion 131 in road 130 is located at the lower section of sample flow runner 111 and turned on sample flow runner 111, lower floor's longitudinal focusing
The outside of stream runner 130 is alignd setting in the vertical direction, with two transverse focusing stream runners 112 and sample flow runner 111,
Alternatively, a left side for two transverse focusing stream runners 112, upper strata longitudinal focusing stream runner 120 and lower floor's longitudinal focusing stream runner 130
Side camber line flow path portion 122 (132) and right side camber line flow path portion 123 (133) are formed respectively to be oppositely arranged and lateral profile identical
Arc, the width of transverse focusing stream runner 112 are respectively greater than the width of sample flow runner 111, left side camber line flow path portion 122
(132) and right side camber line flow path portion 123 (133) width.
Wherein, at least one in the upper lid 240 and lower cover 250 of micro-fluid chip 1 is provided with watch window 245, is easy to light
Learn detection.One in upper lid 240 and lower cover 250 is provided with sample flow inlet 241, delivery outlet 242 and focused flow inlet
243, wherein, sample flow inlet 241 turns on the sample inflow entrance 111a of the first lamellar body 210, the lamellar body of delivery outlet 242 and first
Fluid outlet 111b conductings on 210, focused flow inlet 243 turn on the focusing inflow entrance 112a of micro-channel structure 100, can
Two sheath liquid stream b focused flow inlet 243 is merged into all the way by the conduit outside micro-fluid chip 1 and is connected to one
Liquid flowing source c, so as to realize that single sheath fluid focuses on.
In use, the micro-channel structure 100 of first aspect present invention embodiment both can be as in micro-fluid chip 1
One function module, can also individually designed be a micro-fluid chip for being exclusively used in two dimensional fluid dynamic focus 1, it is manufactured micro-
Fluid chip 1 can be used as a kind of function element, can both coordinate other equipment to form a set of open system platform, can also
Portable instrument or equipment are developed based on this, or substitute the Hydrodynamic focus device in traditional instrument or equipment.
The micro-fluid chip 1 can be used for detecting the life such as cell, embryo, RNA, DNA, protein particulate, microorganism, virus
Thing particulate.In the detection, the sample flow a comprising biological particle stably can be focused on fluid channel by the micro-fluid chip 1
Center, to improve accuracy, sensitivity and the stability of detection.
Other of micro-fluid chip 1 according to embodiments of the present invention are formed and operated for those of ordinary skill in the art
For be all known, be not detailed herein.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " outer ", " up time
The orientation or position relationship of the instruction such as pin ", " counterclockwise ", " axial direction ", " radial direction ", " circumference " be based on orientation shown in the drawings or
Position relationship, it is for only for ease of and describes the present invention and simplify description, rather than indicates or imply that signified device or element must
There must be specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity for indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can be expressed or
Implicitly include at least one this feature.In the description of the invention, " multiple " are meant that at least two, such as two, three
It is individual etc., unless otherwise specifically defined.
In the present invention, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " fixation " etc.
Term should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integrally；Can be that machinery connects
Connect or electrically connect or can communicate each other；Can be joined directly together, can also be indirectly connected by intermediary, can be with
It is connection or the interaction relationship of two elements of two element internals, is limited unless otherwise clear and definite.For this area
For those of ordinary skill, the concrete meaning of above-mentioned term in the present invention can be understood as the case may be.
In the present invention, unless otherwise clearly defined and limited, fisrt feature can be with "above" or "below" second feature
It is that the first and second features directly contact, or the first and second features pass through intermediary mediate contact.Moreover, fisrt feature exists
Second feature " on ", " top " and " above " but fisrt feature are directly over second feature or oblique upper, or be merely representative of
Fisrt feature level height is higher than second feature.Fisrt feature second feature " under ", " lower section " and " below " can be
One feature is immediately below second feature or obliquely downward, or is merely representative of fisrt feature level height and is less than second feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment or example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the different embodiments or example and the feature of different embodiments or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (13)
- A kind of 1. micro-channel structure that two dimensional fluid dynamic focus is realized using single channel sheath fluid, it is characterised in that including：Sample flow runner, the sample flow runner form sample in fore-and-aft direction along linear extension, the both ends of the sample flow runner This inflow entrance and fluid outlet；Upper strata longitudinal focusing stream runner, the folded top for being located at the sample flow runner of the upper strata longitudinal focusing stream runner and with institute State sample flow flow passage；Lower floor's longitudinal focusing stream runner, the folded lower section for being located at the sample flow runner of lower floor's longitudinal focusing stream runner and with institute State sample flow flow passage；Two transverse focusing stream runners, described two transverse focusing stream runners are respectively provided at the left side and the right side of the sample flow runner Side, and respectively with the sample flow runner, the upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream flow passage, Each transverse focusing stream runner is gradually reduced in liquid flow path direction, from rear to preceding width, each transverse focusing stream stream The Breadth Maximum in road is more than the width of the sample flow runner；Two focusing inflow entrances, described two focusing inflow entrances are indulged with two the transverse focusing stream runners, the upper stratas respectively To focused flow runner and lower floor's longitudinal focusing stream flow passage, described two focusing inflow entrances pass through pipeline and a liquid stream Source connects.
- 2. the micro-channel structure according to claim 1 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, described two transverse focusing stream runners are symmetrical on the longitudinal center section of the sample flow runner.
- 3. the micro-channel structure according to claim 1 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, in the transverse direction of the upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream runner on the sample flow runner Heart section is symmetrical above and below.
- 4. the micro-channel structure according to claim 1 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, the upper strata longitudinal focusing stream runner and/or lower floor's longitudinal focusing stream runner include：Line flowing channel portion, the line flowing channel portion and the sample flow runner alignment in the vertical direction；Left side camber line flow path portion, the left side camber line flow path portion are located at the left side in the line flowing channel portion, and the left side camber line The front end of flow path portion is tangent with the rear end in the line flowing channel portion and connects；Right side camber line flow path portion, the right side camber line flow path portion are located at the right side in the line flowing channel portion, and the right side camber line The front end of flow path portion is tangent with the rear end in the line flowing channel portion and connects,The outboard wheel profile of the upper strata longitudinal focusing stream runner and lower floor's longitudinal focusing stream runner and described two transverse directions The opposite outboard wheel profile of focused flow runner alignment in the vertical direction.
- 5. the micro-channel structure according to claim 4 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, the front end of the left side camber line flow path portion connects with the front end of the right side camber line flow path portion, and the left side camber line stream Angle between the inner side contour line of road portion and the right side camber line flow path portion intersection is α, 50 °≤α≤80 °.
- 6. the micro-channel structure according to claim 4 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, the front end face in the line flowing channel portion forms the forwardly convex on-plane surface of plane or middle part.
- 7. the micro-channel structure according to claim 4 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, described two transverse focusing stream runners form the arc being disposed opposite to each other respectively, and each transverse focusing stream runner Width is more than the width of the left side camber line flow path portion and the right side camber line flow path portion.
- 8. the micro-channel structure according to claim 7 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature Be, the opposite outboard wheel profile and relative inner side contour line of two transverse focusing stream runners respectively with the sample Flow the tangent setting of outer contour of runner.
- 9. the micro-channel structure according to claim 1 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, the longitudinal thickness of the sample flow runner is equal with the longitudinal thickness of two transverse focusing stream runners, the upper strata The longitudinal thickness of longitudinal focusing stream runner and lower floor's longitudinal focusing stream runner is equal.
- 10. the micro-channel structure according to claim 9 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, the longitudinal thickness of the sample flow runner and the upper strata longitudinal focusing stream runner/lower floor longitudinal focusing stream runner Longitudinal thickness it is equal.
- 11. the micro-channel structure according to claim 9 that two dimensional fluid dynamic focus is realized using single channel sheath fluid, its feature It is, the longitudinal thickness of the sample flow runner and the upper strata longitudinal focusing stream runner/lower floor longitudinal focusing stream runner Longitudinal thickness it is unequal.
- 12. a kind of micro-fluid chip, it is characterised in that including the utilization single channel sheath according to any one of claim 1-11 Liquid realizes the micro-channel structure of two dimensional fluid dynamic focus.
- 13. micro-fluid chip according to claim 12, it is characterised in that including：First lamellar body, formed with the sample flow runner and two transverse focusing stream runners on first lamellar body；Second lamellar body, the folded top for being located at first lamellar body of second lamellar body, and formed with described on second lamellar body Upper strata longitudinal focusing stream runner；3rd lamellar body, the folded lower section for being located at first lamellar body of the 3rd lamellar body, and formed with described on the 3rd lamellar body Lower floor's longitudinal focusing stream runner；Upper lid, the upper folded top for being located at second lamellar body of lid；Lower cover, the folded lower section for being located at the 3rd lamellar body of the lower cover, at least one in the upper lid and the lower cover are provided with Focused flow inlet, the sample flow inlet connected with the sample inflow entrance and the delivery outlet connected with the fluid outlet.
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| CN201610210300.1A CN105854963B (en) | 2016-04-06 | 2016-04-06 | The micro-channel structure and micro-fluid chip of two dimensional fluid dynamic focus are realized using single channel sheath fluid |
| PCT/CN2016/105081 WO2017173820A1 (en) | 2016-04-06 | 2016-11-08 | Microchannel structure utilizing single-path sheath fluid for implementing two-dimensional hydrodynamic focusing and microfluidic chip |
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| CN111735854A (en) * | 2020-06-18 | 2020-10-02 | 东南大学 | Multi-mode accurately-focused electrical impedance flow cytometry detection device and preparation method thereof |
| CN113814008A (en) * | 2020-08-28 | 2021-12-21 | 上海交通大学 | Microfluidic channel structure, chip, particle ordered arrangement method and application |
| CN115041243B (en) * | 2022-05-19 | 2023-11-10 | 珠海大略科技有限公司 | Micro-fluidic device for particle sorting and high concentration based on micropores |
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| CN1886315A (en) * | 2003-10-30 | 2006-12-27 | 赛托诺姆公司 | Multilayer hydrodynamic sheath flow structure |
| US8563325B1 (en) * | 2009-09-29 | 2013-10-22 | Sandia Corporation | Coaxial microreactor for particle synthesis |
| CN105149019A (en) * | 2015-06-29 | 2015-12-16 | 清华大学 | Micro-channel structure used for two-dimensional hydrodynamic focusing and microfluid chip |
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| EP2972212B1 (en) * | 2013-03-14 | 2022-12-21 | Cytonome/ST, LLC | Hydrodynamic focusing apparatus and methods |
| CN105854963B (en) * | 2016-04-06 | 2017-11-21 | 清华大学 | The micro-channel structure and micro-fluid chip of two dimensional fluid dynamic focus are realized using single channel sheath fluid |
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| CN1886315A (en) * | 2003-10-30 | 2006-12-27 | 赛托诺姆公司 | Multilayer hydrodynamic sheath flow structure |
| US8563325B1 (en) * | 2009-09-29 | 2013-10-22 | Sandia Corporation | Coaxial microreactor for particle synthesis |
| CN105149019A (en) * | 2015-06-29 | 2015-12-16 | 清华大学 | Micro-channel structure used for two-dimensional hydrodynamic focusing and microfluid chip |
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