CN105842199A - Sealed blood identification method based on supercontinuum diffuse integrated laser spectrum - Google Patents
Sealed blood identification method based on supercontinuum diffuse integrated laser spectrum Download PDFInfo
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- CN105842199A CN105842199A CN201610167751.1A CN201610167751A CN105842199A CN 105842199 A CN105842199 A CN 105842199A CN 201610167751 A CN201610167751 A CN 201610167751A CN 105842199 A CN105842199 A CN 105842199A
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- 239000008280 blood Substances 0.000 title claims abstract description 105
- 210000004369 blood Anatomy 0.000 title claims abstract description 104
- 238000001228 spectrum Methods 0.000 title claims abstract description 55
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241001465754 Metazoa Species 0.000 claims abstract description 25
- 238000007405 data analysis Methods 0.000 claims abstract description 24
- 238000004611 spectroscopical analysis Methods 0.000 claims abstract description 15
- 210000002381 plasma Anatomy 0.000 claims abstract description 14
- 210000002966 serum Anatomy 0.000 claims abstract description 13
- 230000003595 spectral effect Effects 0.000 claims description 66
- 238000012360 testing method Methods 0.000 claims description 62
- 230000033001 locomotion Effects 0.000 claims description 27
- 239000000470 constituent Substances 0.000 claims description 25
- 239000013307 optical fiber Substances 0.000 claims description 25
- 230000003287 optical effect Effects 0.000 claims description 24
- 230000005540 biological transmission Effects 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 12
- 230000003993 interaction Effects 0.000 claims description 11
- 238000000576 coating method Methods 0.000 claims description 10
- 239000011248 coating agent Substances 0.000 claims description 9
- 238000004458 analytical method Methods 0.000 claims description 8
- 239000000835 fiber Substances 0.000 claims description 8
- 238000012850 discrimination method Methods 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 230000005693 optoelectronics Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
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- 241000894007 species Species 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000012847 principal component analysis method Methods 0.000 abstract description 2
- 238000001429 visible spectrum Methods 0.000 abstract description 2
- 238000002329 infrared spectrum Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 4
- 230000004313 glare Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 239000003146 anticoagulant agent Substances 0.000 description 2
- 229940127219 anticoagulant drug Drugs 0.000 description 2
- 238000004159 blood analysis Methods 0.000 description 2
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- 229940125691 blood product Drugs 0.000 description 2
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- 238000005538 encapsulation Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
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- 238000005070 sampling Methods 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/4738—Diffuse reflection, e.g. also for testing fluids, fibrous materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
Abstract
The invention discloses a sealed blood identification method based on supercontinuum diffuse integrated laser spectrum. Spectroscopic data outputted from a visible spectrum spectrometer and an Infrared spectrum spectrometer are sent through a USB interface into a master control and data analysis system to be stored, and principal component value of the spectroscopic data is calculated on the basis of a principal component analysis method; a feature vector of the principal component value of blood to be tested is compared with a cluster center feature vector of a principal component spatial database of animal's whole-blood, blood plasma and serum so as to determine blood type of the blood to be tested, and the principal component value of the blood to be tested is compared with a cluster center feature vector of a principal component spatial database of human with the blood type different from the animal species so as to determine species. Then, blood type and species of the blood to be tested are determined. The invention has the following beneficial effects: the blood identifier is suitable for automatic identification of whole blood, blood plasma and serum; and identification accuracy is high.
Description
Technical field
The present invention relates to a kind of people and animal blood classifying apparatus and method, particularly relate to a kind of based on super continuous
The sealing blood discrimination method of unrestrained comprehensive laser spectrum.
Background technology
China all takes credit system for customs's import and export of blood and other kinds biomaterial at present, and
The verity of different kind organism material cannot directly be detected the most for various reasons.Particularly relate to blood sample
Such special material, open contact many times condition of sampling is not allowed to.On the one hand blood
Liquid sample may be detected operational pollution;On the other hand the virulence factor that blood sample self may carry can be right
Testing staff causes occupational exposure.In view of the foregoing, the exploitation contactless Fast Detection Technique of blood sample
Method is the most urgent.
Human blood is similar to animal's whole blood main constituent, is mainly made up of hemocyte and blood plasma, all takes on a red color,
Naked eyes are difficult to distinguish, but people and animal blood cell and the pattern of blood plasma and composition have technicality, selection
Appropriate method just can distinguish human blood and animal blood.The detection method of classical contact can be examined
Measure part blood parameters, the blood between different genera can be carried out according to these blood parameters and differentiate.Mesh
Before, commercial use more blood to differentiate product is all based on greatly flow cytometry, contact need to be carried out and decline sampling
Obtain the parameter of the representative phylogenetic feature of blood.It is a problem being rich in challenge that contactless blood differentiates,
Because the major part either whole blood that is sealed in cuvette of species or the sample such as serum, blood plasma, its
Ultraviolet, spectral coverage visible, infrared, the optical characteristics externally reflected is the most similar, in the detection of non-sampled,
The means relying on optics are differentiated extremely difficult.
First, in test tube, the blood products of encapsulation may be containing the anticoagulant of different components, including heparin, second
Ethylenediaminotetraacetate (edetate) (edta salt), citrate, oxalates etc., tube material is probably quartz in addition
Glass or PET etc., major part test tube the most all labels.These interference factors, will have a strong impact on
The optical property of blood so that conventional optics and spectrographic technique are helpless on sealing test tube blood differentiates.
For these reasons, exploitation one seals test tube blood for people and animal, including whole blood, serum,
The contactless taxonomic history instrument of blood plasma and method are urgent need to solve the problems.
For this problem, the present invention propose a kind of based on visible to the super continuous unrestrained comprehensive laser spectrum of mid-infrared
For sealing instrument and the method for the contactless discriminating of test tube blood sample, use wide spectrum super continuous spectrums laser
The integrating sphere of source and particular design is core instrument hardware structure, obtains the unrestrained comprehensive laser spectrum of different sample
Data, set up different plant species, different test tube, the spectra database of different blood, and based on principal component analysis
Method (principal components analysis is called for short PCA) calculates these spectroscopic datas and obtains principal component analysis
Shot chart, obtains people and animal's whole blood, blood plasma, the cluster areas of serum, by these regions in shot chart
As differentiating that criterion carries out people and seals blood sample noncontact discriminating with animal test tube.
Summary of the invention
It is an object of the invention to provide a kind of based on visible to the super continuous unrestrained comprehensive laser spectrum of mid-infrared
For sealing test tube blood sample contactless mirror method for distinguishing, people and animal's whole blood, blood plasma and blood can be carried out
Clear automatic identification, meets the detection quarantine departments demand to blood products Rough Inspection.
The technical scheme is that and be achieved in, sealing blood based on super continuous unrestrained comprehensive laser spectrum
The hardware system of liquid identifier is mainly by power supply module, visible spectral coverage spectrogrph, infrared spectral coverage spectrogrph, Y
Shape optical fiber, master control and data analysis system, integrating sphere, test tube mechanical hand, connecting rod, guide rail, mechanical hand
Motion controller, sample room outer cover plate, base plate, support, light source chamber outer cover plate, fiber optic collimator joint, swash
Light device tail optical fiber, super continuous spectrums laser constitution.
Wherein, integrating sphere is made up of integrating sphere right-hand part and two, integrating sphere left side hemisphere, and they are by long-pending
Bulb separation joint couples, and is fixed on base plate.Integrating sphere inwall coating diffuse-reflectance coating, comparison
The light being mapped to inwall plays the effect of the even light of diffuse-reflectance.Integrating sphere right-hand part has integrating sphere sample well, sample
Outdoor cover plate is arranged on base plate and integrating sphere right-hand part, is formed and closes Proteins room, to eliminate veiling glare
Impact.Having circular hole above the outer cover plate of sample room, handling main shaft passes in integrating sphere sample well and circular hole
The heart, and vertical with base plate.Integrating sphere left side has integrating sphere light source hole and integrating sphere optical fiber interface.
Visible spectral coverage spectrogrph and infrared spectral coverage spectrogrph all use same light spectrometer optical fiber interface, Y shape optical fiber
One end couples with integrating sphere optical fiber interface, can collect from the diffuse-reflectance light receiving optical axis, and another two ends are respectively
Couple with visible spectral coverage spectrogrph and infrared spectral coverage spectrogrph.
Visible and the infrared spectral coverage super continuous spectrums pulse laser that super continuous spectrums laser instrument sends is through laser instrument tail optical fiber
Transmission, exports super continuous spectrums pulse collimated laser beam after then carrying out beam collimation by fiber optic collimator joint,
And enter integrating sphere along launching optical axis through integrating sphere light source hole.
Fiber optic collimator joint is fixed on base plate by support, and by light source chamber outer cover plate and integrating sphere left side
Shell couples, and is formed and closes spatial light source chamber, to eliminate the impact of veiling glare.
Guide rail and base plate right angle setting, test tube mechanical hand is coupled with guide rail by connecting rod and can be transported at mechanical hand
Along slide under movement controller control.Blood to be checked is encapsulated in test tube by test tube cap.Test tube mechanical hand exists
Under robot movement controller controls, test tube cap can be firmly grasped and drive test tube to transport up and down along handling major axes orientation
Dynamic.
Power supply module in order to visible spectral coverage spectrogrph, infrared spectral coverage spectrogrph, master control and data analysis system,
Super continuous spectrums laser instrument and robot movement controller are powered.Master control and data analysis system are in order to can
See that spectral coverage spectrogrph, infrared spectral coverage spectrogrph, super continuous spectrums laser instrument and robot movement controller are controlled
System, and receive visible spectral coverage spectrogrph and the spectroscopic data of infrared spectral coverage spectrogrph output by USB interface
Process and analyze.Master control and data analysis system included touch screen human-computer interaction interface, be used for and user
Man-machine interaction, accept the instruction of user and export result to user.
The blood analysis method sealing blood identifier based on super continuous unrestrained comprehensive laser spectrum the steps include:
Power-on module, to visible spectral coverage spectrogrph, infrared spectral coverage spectrogrph, master control and data analysis system
System, super continuous spectrums laser instrument and robot movement controller are powered.
User starts test mastery routine by touch screen human-computer interaction interface.Now, master control and data analysis system
System sends sample introduction and instructs to robot movement controller, and robot movement controller controls test tube mechanical hand and captures
Test tube sample introduction enters integrating sphere to it along handling major axes orientation.Test tube bottom position higher than launch optical axis and with connect
Receipts optical axis is non-intersect, it is to avoid laser beam direct irradiation test tube to be checked, and avoids the transflector light of test tube direct
Along launching optical axis transmission and being collected by Y shape optical fiber.
Master control and data analysis system send instruction and start super continuous spectrums laser instrument, visible spectral coverage spectrogrph and red
Outer spectral coverage spectrogrph.The super continuous spectrums collimated laser beam of super continuous spectrums laser instrument output enters long-pending along launching optical axis
Bulb separation, after laser beam irradiation to diffuse-reflectance coating, its reflection light is diffuse-reflectance, i.e. along each side in integrating sphere
To transmission, become uniform light.After test tube is irradiated by the super continuous spectrums light of different directions, test tube (note:
Including its material with label outward) with the diffuse-reflectance of blood to be checked, diffusing transmission, absorb, launch after light
To transmit along space any direction, and run into diffuse-reflectance coating diffuse-reflectance to any direction, it is in integrating sphere
Light transmission has the impact of unrestrained integrated spectral.
After being collected along the diffuse-reflectance light launching optical axis transmission by Y shape optical fiber, deliver to visible spectral coverage spectrum respectively
Instrument and infrared spectral coverage spectrogrph carry out opto-electronic conversion and become spectroscopic data.The sampled point of visible spectral coverage spectrogrph is
N1=1300.The sampled point of infrared spectral coverage spectrogrph is N2.Visible spectral coverage spectrogrph and infrared spectral coverage spectrum
Common N=N1+N2 the spectroscopic data of instrument output delivers to master control and data analysis system is deposited through USB interface
Storage.And it is N number of to calculate this based on PCA (principal components analysis is called for short PCA)
M main constituent numerical value of spectroscopic data, carries out dimension-reduction treatment, and the value of M takes 7, i.e. 7 main constituents.
By M main constituent numerical value of blood to be checked, obtain its characteristic vector in M dimension main constituent space,
This feature vector and M are tieed up the people obtained by this blood identifier and animal's whole blood, the blood in main constituent space
Slurry, serum M dimension main constituent spatial database cluster centre characteristic vector contrast, according to feature to
First amount similarity determines the type blood of blood to be checked, is i.e. whole blood, blood plasma or serum.Then, then
By M main constituent numerical value of blood to be checked, with the M dimension of the people under this type blood with animal different genera
The cluster centre characteristic vector of main constituent spatial database contrasts, and determines according to characteristic vector similarity again
Kind, is i.e. people or animal blood, if animal blood, is which kind of animal blood, so far, completes
The type blood of blood to be checked and the judgement of kind.
Then, the visible infrared excess of blood to be checked is overflow comprehensive laser spectrum by master control and data analysis system continuously
Curve and result of determination show on touch screen human-computer interaction interface, with for reference.Master control and data are divided
Analysis system sends out sample and instructs to robot movement controller, and robot movement controller controls test tube mechanical hand
Drive test tube to it along handling major axes orientation removal integrating sphere and sample room, complete whole test process.
The invention has the beneficial effects as follows, have employed the integrating sphere of particular design, either blood plasma, serum are transparent
The diffusing transmission of liquid, or whole blood and the diffuse-reflectance of test tube label, and the unrestrained suction of whole test sample is penetrated and
The unrestrained wide spectrum signal launched all can receive, be i.e. applicable to all kinds of blood, all kinds of material test tube, all kinds of not
Uniformly gather with the spectroscopic data under anticoagulant and different label condition.Have employed visible to mid-infrared Gao Gong
The bispectrum section spectrogrph of rate super continuous spectrums lasing light emitter and correspondence, obtains wide spectrum and overflows comprehensive laser spectrum data,
The trickle optical difference of encapsulation blood can be caught, discriminating accuracy can be improved.Due to hardware configuration and
The robustness of software analysis, the blood identifier of the present invention is applicable to the automatic knowledge of whole blood, blood plasma and serum
Not.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the present invention, in figure: 1 power supply module;Spectral coverage spectrogrph seen from 2;
3 infrared spectral coverage spectrogrphs;4 spectrogrph optical fiber interfaces;5 Y shape optical fiber;6 USB connect
Mouthful;7 master controls and data analysis system;8 integrating sphere optical fiber interfaces;9 integrating sphere joints;
10 test tube mechanical hands;11 connecting rods;12 guide rails;13 robot movement controllers;
14 test tube caps;15 test tubes;16 circular holes;17 integrating sphere sample wells;18 samples
Outdoor cover plate;19 blood to be checked;20 receive optical axis;21 sample rooms;22 base plates;
23 integrating sphere right-hand parts;24 diffuse-reflectance coatings;25 launch optical axis;26 integrating spheres are left
Half portion;27 light source chambers;28 integrating sphere light source holes;29 supports;30 light source chamber enclosing covers
Plate;31 fiber optic collimator joints;32 laser instrument tail optical fibers;33 touch screen human-computer interaction interfaces;
34 super continuous spectrums laser instrument;35 integrating spheres;36 handling main shafts.
Detailed description of the invention
The hardware system structure sealing blood identifier based on super continuous unrestrained comprehensive laser spectrum as shown in Figure 1,
Hardware system is mainly by power supply module 1, visible spectral coverage spectrogrph 2, infrared spectral coverage spectrogrph 3, Y shape optical fiber
5, master control and data analysis system 7, integrating sphere 35, test tube mechanical hand 10, connecting rod 11, guide rail 12,
Robot movement controller 13, sample room outer cover plate 18, base plate 22, support 29, light source chamber outer cover plate 30,
Fiber optic collimator joint 31, laser instrument tail optical fiber 32, super continuous spectrums laser instrument 34 form.
Wherein, integrating sphere 35 is made up of integrating sphere right-hand part 23 and 26 two, integrating sphere left side hemisphere,
They are coupled by integrating sphere joint 9, and are fixed on base plate 22.Integrating sphere 35 inwall is coated with
Cover diffuse-reflectance coating 24, the light being irradiated to inwall is played the effect of the even light of diffuse-reflectance.Integrating sphere right-hand part
23 have integrating sphere sample well 17, and sample room outer cover plate 18 is arranged on base plate 22 and integrating sphere right-hand part 23
On, formed and close Proteins room 21, to eliminate the impact of veiling glare.Open above sample room outer cover plate 18
Have a circular hole 16, handling main shaft 36 through integrating sphere sample well 17 and the center of circular hole 16, and with base plate 22
Vertically.Integrating sphere left side 26 has integrating sphere light source hole 28 and integrating sphere optical fiber interface 8 (note: this enforcement
Example is SMA905 interface).
Visible spectral coverage spectrogrph 2 and infrared spectral coverage spectrogrph 3 all use same light spectrometer optical fiber interface 4 (note:
The present embodiment is SMA905 interface), Y shape optical fiber 5 one end couples with integrating sphere optical fiber interface 8, can
Collect from the diffuse-reflectance light receiving optical axis 20, another two ends respectively with visible spectral coverage spectrogrph 2 and infrared
Spectral coverage spectrogrph 3 couples.
Super continuous spectrums laser instrument 34 (note: its spectral region 400nm-2400nm in the present embodiment, power 1W,
Repetition 1MHz, pulsewidth 150ps) the visible and infrared spectral coverage super continuous spectrums pulse laser that sends is through laser tail
Fine 32 transmission, export super continuous spectrums pulse after then carrying out beam collimation by fiber optic collimator joint 31 and collimate
Laser beam, and enter integrating sphere 35 along launching optical axis 25 through integrating sphere light source hole 28.
Fiber optic collimator joint 31 is fixed on base plate 22 by support 29, and by light source chamber outer cover plate 30 with
Integrating sphere left side 26 shell couples, and is formed and closes spatial light source chamber 27, to eliminate the impact of veiling glare.
Guide rail 12 and base plate 22 right angle setting, test tube mechanical hand 10 is by connecting rod 11 and guide rail 12
Connect and can slide along guide rail 12 under robot movement controller 13 controls.Blood to be checked 19 is by test tube cap
14 are encapsulated in test tube 15.Test tube mechanical hand 10, under robot movement controller 13 controls, can be firmly grasped
Test tube cap 14 also drives test tube 15 to move up and down along handling main shaft 36 direction.
Power supply module 1 is in order to divide visible spectral coverage spectrogrph 2, infrared spectral coverage spectrogrph 3, master control and data
Analysis system 7, super continuous spectrums laser instrument 34 and robot movement controller 13 are powered.Master control and data
Analysis system 7 is in order to visible spectral coverage spectrogrph 2, infrared spectral coverage spectrogrph 3, super continuous spectrums laser instrument 34
And robot movement controller 13 is controlled, and receive visible spectral coverage spectrogrph 2 by USB interface 6
And the spectroscopic data of infrared spectral coverage spectrogrph 3 output processes and analyzes.Master control and data analysis system 7
Included touch screen human-computer interaction interface 33, for the man-machine interaction with user, accepts the instruction of user and exports
Result is to user.
The blood analysis method sealing blood identifier based on super continuous unrestrained comprehensive laser spectrum the steps include:
Visible spectral coverage spectrogrph 2, infrared spectral coverage spectrogrph 3, master control and data are divided by power-on module 1
Analysis system 7, super continuous spectrums laser instrument 34 and robot movement controller 13 are powered.
User starts test mastery routine by touch screen human-computer interaction interface 33.Now, master control and data are divided
Analysis system 7 sends sample introduction and instructs to robot movement controller 13, and robot movement controller 13 controls examination
Pipe mechanical hand 10 captures test tube 15 sample introduction and enters integrating sphere 35 to it along handling main shaft 36 direction.Test tube 15
Bottom position higher than launch optical axis 25 and with receive optical axis 20 non-intersect, it is to avoid laser beam direct irradiation is to be checked
Test tube 15, and avoid the transflector light of test tube 15 directly to transmit and by Y shape optical fiber 5 along transmitting optical axis 25
Collect.
Master control and data analysis system 7 send instruction and start super continuous spectrums laser instrument 34, visible spectral coverage spectrogrph
2 and infrared spectral coverage spectrogrph 3.The super continuous spectrums collimated laser beam of super continuous spectrums laser instrument 34 output is along launching
Optical axis 25 enters integrating sphere 35, and after laser beam irradiation to diffuse-reflectance coating 24, its reflection light is diffuse-reflectance,
I.e. transmit along all directions in integrating sphere 35, become uniform light.Test tube 15 is by the super company of different directions
After continuous spectrum light irradiates, test tube 15 (note: include its material and label outward) is unrestrained anti-with blood 19 to be checked
Penetrate, diffusing transmission, absorb, launch after light will transmit along space any direction, run into diffuse-reflectance coating 24
Diffuse-reflectance is to any direction, and the light in integrating sphere 35 is transmitted by it has the impact of unrestrained integrated spectral.
After being collected along the diffuse-reflectance light launching optical axis 25 transmission by Y shape optical fiber 5, deliver to visible spectrum respectively
Section spectrogrph 2 and infrared spectral coverage spectrogrph 3 carry out opto-electronic conversion and become spectroscopic data.In this specific embodiment
The spectral region of visible spectral coverage spectrogrph 2 is 400-750nm, and sampled point is N1=1300.Infrared spectral coverage light
The spectral region of spectrometer 3 is 855-1744nm, and sampled point is N2=512.Visible spectral coverage spectrogrph 2 and red
Common N=N1+N2 the spectroscopic data of outer spectral coverage spectrogrph 3 output delivers to master control and data through USB interface 6
Analysis system 7 stores.And based on PCA (principal components analysis, letter
Claim PCA) calculate this N number of spectroscopic data M main constituent numerical value (note: M=7 in the present embodiment, i.e.
Calculate 7 main constituent numerical value), carry out dimension-reduction treatment.
By M main constituent numerical value of blood 19 to be checked, obtain its feature in M dimension main constituent space to
Amount, by the people and the animal's whole blood that are obtained by this blood identifier in this feature vector and M dimension main constituent space,
The cluster centre characteristic vector of blood plasma, serum M dimension main constituent spatial database contrasts, according to feature
First vector similarity determines the type blood of blood 19 to be checked, is i.e. whole blood, blood plasma or serum.So
After, then by M main constituent numerical value of blood 19 to be checked, the most of the same race with animal with the people under this type blood
The cluster centre characteristic vector of the M dimension main constituent spatial database belonged to contrasts, according to characteristic vector phase
Determine kind again like degree, be i.e. people or animal blood, if animal blood, be which kind of animal blood,
So far, the type blood of blood 19 to be checked and the judgement of kind are completed.
Then, master control and data analysis system 7 are unrestrained comprehensive sharp continuously by the visible infrared excess of blood 19 to be checked
The light curve of spectrum and result of determination show on touch screen human-computer interaction interface 33, with for reference.Main
Control and data analysis system 7 send out sample and instruct to robot movement controller 13, robot movement controller
13 control test tube mechanical hand 10 drives test tube 15 to it along handling main shaft 36 direction removal integrating sphere 35 and sample
Product room 21, completes whole test process.
Claims (1)
1. a sealing blood discrimination method based on super continuous unrestrained comprehensive laser spectrum, the method be based on
The blood that seals of super continuous unrestrained comprehensive laser spectrum differentiates realization, described sealing blood identifier on instrument
Device includes power supply module (1), visible spectral coverage spectrogrph (2), infrared spectral coverage spectrogrph (3), Y shape optical fiber
(5), master control and data analysis system (7), integrating sphere (35), test tube mechanical hand (10), connecting rod (11),
Guide rail (12), robot movement controller (13), sample room outer cover plate (18), base plate (22), support
(29), light source chamber outer cover plate (30), fiber optic collimator joint (31), laser instrument tail optical fiber (32), super continuous
Spectrum laser instrument (34);It is characterized in that sealing blood discrimination method as follows:
Power-on module, to visible spectral coverage spectrogrph, infrared spectral coverage spectrogrph, master control and data analysis system
System, super continuous spectrums laser instrument and robot movement controller are powered;
User starts test mastery routine, now, master control and data analysis system by touch screen human-computer interaction interface
System sends sample introduction and instructs to robot movement controller, and robot movement controller controls test tube mechanical hand and captures
Test tube sample introduction enters integrating sphere to it along handling major axes orientation;Test tube bottom position higher than launch optical axis and with connect
Receipts optical axis is non-intersect, it is to avoid laser beam direct irradiation test tube to be checked, and avoids the transflector light of test tube direct
Along launching optical axis transmission and being collected by Y shape optical fiber;
Master control and data analysis system send instruction and start super continuous spectrums laser instrument, visible spectral coverage spectrogrph and red
Outer spectral coverage spectrogrph, the super continuous spectrums collimated laser beam of super continuous spectrums laser instrument output enters long-pending along launching optical axis
Bulb separation, after laser beam irradiation to diffuse-reflectance coating, its reflection light is diffuse-reflectance, i.e. along each side in integrating sphere
To transmission, become uniform light.After test tube is irradiated by the super continuous spectrums light of different directions, test tube, examination
Pipe is outer label with the diffuse-reflectance of blood to be checked, diffusing transmission, absorb, launch after light will be any along space
Direction is transmitted, and runs into diffuse-reflectance coating diffuse-reflectance to any direction, and the light in integrating sphere is transmitted by it to be had
Unrestrained integrated spectral impact;
After being collected along the diffuse-reflectance light launching optical axis transmission by Y shape optical fiber, deliver to visible spectral coverage spectrum respectively
Instrument and infrared spectral coverage spectrogrph carry out opto-electronic conversion and become spectroscopic data, it is seen that the sampled point of spectral coverage spectrogrph is
N1=1300;The sampled point of infrared spectral coverage spectrogrph is N2=512;Visible spectral coverage spectrogrph and infrared spectral coverage
Common N=N1+N2 the spectroscopic data of spectrogrph output delivers to master control and data analysis system enters through USB interface
Row storage, and M main constituent numerical value of this N number of spectroscopic data is calculated based on PCA, carry out
Dimension-reduction treatment, the value of M takes 7, i.e. 7 main constituents;
By M main constituent numerical value of blood to be checked, obtain its characteristic vector in M dimension main constituent space,
This feature vector and M are tieed up the people obtained by this blood identifier and animal's whole blood, the blood in main constituent space
Slurry, serum M dimension main constituent spatial database cluster centre characteristic vector contrast, according to feature to
First amount similarity determines the type blood of blood to be checked, is i.e. whole blood, blood plasma or serum;Then, then
By M main constituent numerical value of blood to be checked, with the M dimension of the people under this type blood with animal different genera
The cluster centre characteristic vector of main constituent spatial database contrasts, and determines according to characteristic vector similarity again
Kind, is i.e. people or animal blood, if animal blood, is which kind of animal blood, so far, completes
The type blood of blood to be checked and the judgement of kind;
Then, the visible infrared excess of blood to be checked is overflow comprehensive laser spectrum by master control and data analysis system continuously
Curve and result of determination show on touch screen human-computer interaction interface, with for reference.Master control and data are divided
Analysis system sends out sample and instructs to robot movement controller, and robot movement controller controls test tube mechanical hand
Drive test tube to it along handling major axes orientation removal integrating sphere and sample room, complete whole test process.
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