CN105842176A - Blood identification instrument based on ultraviolet fluorescence super-continuum diffuse comprehensive spectrum - Google Patents
Blood identification instrument based on ultraviolet fluorescence super-continuum diffuse comprehensive spectrum Download PDFInfo
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- CN105842176A CN105842176A CN201610167557.3A CN201610167557A CN105842176A CN 105842176 A CN105842176 A CN 105842176A CN 201610167557 A CN201610167557 A CN 201610167557A CN 105842176 A CN105842176 A CN 105842176A
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- optical fiber
- spectrogrph
- spectral coverage
- laser
- integrating sphere
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
- G01N2021/3148—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths using three or more wavelengths
Abstract
The invention discloses a blood identification instrument based on an ultraviolet fluorescence super-continuum diffuse comprehensive spectrum. A hardware system of the identification instrument comprises a power supply module, a visible spectrum band spectrometer, an infrared spectrum band spectrometer, an ultraviolet narrow linewidth laser, Y-shaped coaxial optical fiber, an optical fiber collimating lens, a object lens coupling, a micro-objective lens, an objective lens bracket, an optical fiber coupling, two-in and two-out optical fiber, a main control and data analysis system, an integrating sphere, a test tube manipulator, a connecting rod, a guide rail, a manipulator motion controller, a sample chamber outer cover plate, a base plate, a bracket, a light source chamber outer cover plate, an optical fiber collimating joint, a laser tail fiber, and a super-continuum spectrum laser. The blood identification instrument provided by the invention can be suitable for automatic identification of whole blood, plasma and serum.
Description
Technical field
The present invention relates to a kind of people and animal blood classifying apparatus and method, particularly relate to a kind of glimmering based on ultraviolet
Light combine super continuous overflow comprehensive laser spectrum be used for seal the contactless discriminating of test tube blood sample instrument and
Method.
Background technology
China all takes credit system for customs's import and export of blood and other kinds biomaterial at present, and
The verity of different kind organism material cannot directly be detected the most for various reasons.Particularly relate to blood sample
Such special material, open contact many times condition of sampling is not allowed to.On the one hand blood
Liquid sample may be detected operational pollution;On the other hand the virulence factor that blood sample self may carry can be right
Testing staff causes occupational exposure.In view of the foregoing, the exploitation contactless Fast Detection Technique of blood sample
Method is the most urgent.
Human blood is similar to animal's whole blood main constituent, is mainly made up of hemocyte and blood plasma, all takes on a red color,
Naked eyes are difficult to distinguish, but people and animal blood cell and the pattern of blood plasma and composition have technicality, selection
Appropriate method just can distinguish human blood and animal blood.The detection method of classical contact can be examined
Measure part blood parameters, the blood between different genera can be carried out according to these blood parameters and differentiate.Mesh
Before, commercial use more blood to differentiate product is all based on greatly flow cytometry, contact need to be carried out and decline sampling
Obtain the parameter of the representative phylogenetic feature of blood.It is a class being rich in challenge that non-contacting seal blood differentiates
Topic, because the major part either whole blood that is sealed in cuvette of species or the sample such as serum, blood plasma,
It is at ultraviolet, spectral coverage visible, infrared, and the optical characteristics externally reflected is the most similar, in the inspection of non-sampled
In survey, the means of optics are relied on to differentiate extremely difficult.
First, in test tube, the blood products of encapsulation may be containing the anticoagulant of different components, including heparin, second
Ethylenediaminotetraacetate (edetate) (edta salt), citrate, oxalates etc., tube material is probably quartz in addition
Glass or PET etc., major part test tube the most all labels.These interference factors, will have a strong impact on
The optical property of blood so that conventional optics and spectrographic technique are helpless on sealing test tube blood differentiates.
For these reasons, exploitation one seals test tube blood for people and animal, including whole blood, serum,
The contactless taxonomic history instrument of blood plasma and correlation method are urgent need to solve the problems.
For this problem, the present invention proposes a kind of visible super continuous unrestrained comprehensive to mid-infrared based on Ultraluminescence combination
Close instrument and the method for sealing the contactless discriminating of test tube blood sample of laser spectrum, in Ultraluminescence
Spectral detection uses ultraviolet narrow linewidth laser, focuses to blood in test tube in conjunction with fiber optic collimator and microcobjective
Liquid sample, and use transmitting to receive the design of coaxial optical fiber end face to gather after blood sample to fluorescent scattering signal;
In super continuous unrestrained comprehensive laser spectrum detection, use the long-pending of wide spectrum super continuous spectrums lasing light emitter and particular design
Bulb separation is core instrument hardware structure, obtains the unrestrained comprehensive laser spectrum data of different sample.The side of the present invention
Method combines both the above laser spectrum detection technique, and uses Y shape fiberoptic connection two to enter scene 2 optical fiber multiplexing light
Spectrometer, it is achieved spectroscopic data information fusion.Set up different plant species, different test tube, the fusion light of different blood
Modal data storehouse, and calculate based on PCA (principal components analysis is called for short PCA)
These merge spectroscopic data and obtain principal component analysis shot chart, obtain people and animal's whole blood, blood in shot chart
Slurry, the cluster areas of serum, using these regions as differentiating that criterion carries out people and seals blood with animal test tube
Sample noncontact differentiates.
Summary of the invention
It is an object of the invention to provide a kind of visible super continuous to mid-infrared based on laser Ultraluminescence combination
The instrument for sealing the contactless discriminating of test tube blood sample of unrestrained comprehensive laser spectrum, can carry out people with dynamic
The automatic identification of thing whole blood, blood plasma and serum, meets the detection quarantine departments demand to blood products Rough Inspection.
The technical scheme is that and be achieved in, combine based on Ultraluminescence and super continuous overflow comprehensive laser
The hardware system sealing blood identifier of spectrum is mainly by power supply module, visible spectral coverage spectrogrph, ultrared spectrum
Section spectrogrph, two enter scene 2 optical fiber, master control and data analysis system, integrating sphere, test tube mechanical hand, connection
Bar, guide rail, robot movement controller, sample room outer cover plate, base plate, support, light source chamber outer cover plate,
Fiber optic collimator joint, laser instrument tail optical fiber, super continuous spectrums laser instrument, objective lens support, microcobjective, object lens join
Connect device, fiber optic collimator mirror, Y shape coaxial optical fiber, fiber optic connector, ultraviolet narrow linewidth laser composition.
Wherein, integrating sphere is made up of integrating sphere right-hand part and two, integrating sphere left side hemisphere, and they are by long-pending
Bulb separation joint couples, and is fixed on base plate.Integrating sphere inwall coating diffuse-reflectance coating, comparison
The light being mapped to inwall plays the effect of the even light of diffuse-reflectance.Integrating sphere right-hand part has integrating sphere sample well, sample
Outdoor cover plate is arranged on base plate and integrating sphere right-hand part, is formed and closes Proteins room, to eliminate veiling glare
Impact.Having circular hole above the outer cover plate of sample room, handling main shaft passes in integrating sphere sample well and circular hole
The heart, and vertical with base plate.Integrating sphere left side has integrating sphere light source hole and integrating sphere optical fiber interface.
Y shape coaxial optical fiber is made up of with fluorescence reception optical fiber ultraviolet emission optical fiber, and both pool a light
Fibre, its fiber end face is coaxial distribution, and central circular is the arrangement of ultraviolet emission optical fiber, outer concentric circular
Ring region territory is the arrangement of fluorescence reception optical fiber, and this geometric configuration can the backward fluorescence that excites of efficient reception Ultra-Violet Laser
Scattered signal.Y shape coaxial optical fiber couples with fiber optic collimator mirror and can realize collimation transmitting and receive, fiber optic collimator
Mirror is connected with microcobjective by object lens coupling, and UV laser beam can focus to fluorescence spectrum test point
And the backscatter signal of fluorescence spectrum test point is collected.Microcobjective is fixedly mounted by objective lens support
On base plate.
Visible spectral coverage spectrogrph and infrared spectral coverage spectrogrph all use same light spectrometer optical fiber interface, and two enter scene 2
Optical fiber is divided into two receiving terminals (i.e. two enter) and two outfans (i.e. scene 2).One receiving terminal and integrating sphere light
Fine interface couples, and can collect from the diffuse-reflectance light receiving optical axis;Another receiving terminal and fluorescence reception light
Fibre is coupled by fiber optic connector, can collect the fluorescence signal from fluorescence reception optical fiber;Two outfans divide
Do not couple with visible spectral coverage spectrogrph and infrared spectral coverage spectrogrph.
Visible and the infrared spectral coverage super continuous spectrums pulse laser that super continuous spectrums laser instrument sends is through laser instrument tail optical fiber
Transmission, exports super continuous spectrums pulse collimated laser beam after then carrying out beam collimation by fiber optic collimator joint,
And enter integrating sphere along launching optical axis through integrating sphere light source hole.
Fiber optic collimator joint is fixed on base plate by support, and by light source chamber outer cover plate and integrating sphere left side
Shell couples, and is formed and closes spatial light source chamber, to eliminate the impact of veiling glare.
The ultraviolet continuous laser beam that ultraviolet narrow linewidth laser sends ultraviolet emission in Y shape coaxial optical fiber
Fiber-optic transfer, from ultraviolet emission optical fiber arrange launch tailing edge Ultra-Violet Laser optical axis transmission, through fiber optic collimator mirror
Collimation, microcobjective can realize after focusing on the ultraviolet induced fluorescence of fluorescence spectrum test point excites (note: purple
Outer laser beam axis intersects with handling main shaft, and its intersection point is fluorescence spectrum test point), fluorescence spectrum test point
Back scattering fluorescence signal successively after microcobjective and fiber optic collimator mirror by Y shape coaxial optical fiber end face outside
Circle concentric annular regions, i.e. fluorescence reception optical fiber arrangement collects, then through fluorescence reception optical fiber, two enter scene 2 light
Fine extremely visible spectral coverage spectrogrph is received and analyzes.
Guide rail and base plate right angle setting, test tube mechanical hand is coupled with guide rail by connecting rod and can be transported at mechanical hand
Along slide under movement controller control.Blood to be checked is encapsulated in test tube by test tube cap.Test tube mechanical hand exists
Under robot movement controller controls, test tube cap can be firmly grasped and drive test tube to transport up and down along handling major axes orientation
Dynamic.
Power supply module in order to visible spectral coverage spectrogrph, infrared spectral coverage spectrogrph, master control and data analysis system,
Super continuous spectrums laser instrument, ultraviolet narrow linewidth laser and robot movement controller are powered.Master control and number
According to the system of analysis in order to visible spectral coverage spectrogrph, infrared spectral coverage spectrogrph, super continuous spectrums laser instrument, ultraviolet
Narrow linewidth laser and robot movement controller are controlled, and receive visible spectral coverage by USB interface
The spectroscopic data of spectrogrph and the output of infrared spectral coverage spectrogrph processes and analyzes.Master control and data analysis system
System included touch screen human-computer interaction interface, for the man-machine interaction with user, accepts the instruction of user and exports
Result is to user.
The hemanalysis sealing blood identifier of super continuous unrestrained comprehensive laser spectrum is combined based on Ultraluminescence
Method the steps include:
(1) instrument starts and super continuous spectrums test sample introduction
Power-on module, to visible spectral coverage spectrogrph, infrared spectral coverage spectrogrph, master control and data analysis system
System, super continuous spectrums laser instrument, ultraviolet narrow linewidth laser and robot movement controller are powered.
User starts test mastery routine by touch screen human-computer interaction interface.Now, master control and data analysis system
System sends sample introduction and instructs to robot movement controller, and robot movement controller controls test tube mechanical hand and captures
Test tube sample introduction enters integrating sphere to it along handling major axes orientation.Test tube bottom position higher than launch optical axis and with connect
Receipts optical axis is non-intersect, it is to avoid laser beam direct irradiation test tube to be checked, and avoids the transflector light of test tube direct
Transmit along transmitting optical axis and entered scene 2 optical fiber by two and collect.
(2) super continuous spectrums laser overflow integrated spectral test
Master control and data analysis system send instruction and start super continuous spectrums laser instrument, visible spectral coverage spectrogrph and red
Outer spectral coverage spectrogrph.The super continuous spectrums collimated laser beam of super continuous spectrums laser instrument output enters long-pending along launching optical axis
Bulb separation, after laser beam irradiation to diffuse-reflectance coating, its reflection light is diffuse-reflectance, i.e. along each side in integrating sphere
To transmission, become uniform light.After test tube is irradiated by the super continuous spectrums light of different directions, test tube (note:
Including its material with label outward) with the diffuse-reflectance of blood to be checked, diffusing transmission, absorb, launch after light
To transmit along space any direction, and run into diffuse-reflectance coating diffuse-reflectance to any direction, it is in integrating sphere
Light transmission has the impact of unrestrained integrated spectral.
After being entered the collection of scene 2 optical fiber along the diffuse-reflectance light launching optical axis transmission by two, deliver to visible spectral coverage respectively
Spectrogrph and infrared spectral coverage spectrogrph carry out opto-electronic conversion and become spectroscopic data.The sampling of visible spectral coverage spectrogrph
Point is N1=1300.The sampled point of infrared spectral coverage spectrogrph is N2=512.Visible spectral coverage spectrogrph and infrared
Common N=N1+N2 the spectroscopic data of spectral coverage spectrogrph output delivers to master control and data analysis system through USB interface
System stores.
(3) fluorescence spectrum test sample introduction
Master control and data analysis system send out sample and instruct to robot movement controller, and robot movement controls
Device controls test tube machinery hand-motion test tube and removes integrating sphere and sample room, to fluorescence to it along handling major axes orientation
Spectrum test point is positioned at the center of test tube blood to be checked, now completes fluorescence spectrum test sample introduction.
(4) Ultra-Violet Laser fluorescence spectrum test
Master control and data analysis system send instruction start ultraviolet narrow linewidth laser, visible spectral coverage spectrogrph and
Infrared spectral coverage spectrogrph.The ultraviolet continuous laser beam collimated focusing post-concentration that ultraviolet narrow linewidth laser sends
Blood to be checked at fluorescence spectrum test point, the fluorescence spectrum signal that ultraviolet narrow linewidth continuous laser is induced
Successively by the outer concentric circular ring region territory of Y shape coaxial optical fiber end face after microcobjective and fiber optic collimator mirror, i.e.
The arrangement of fluorescence reception optical fiber is collected, then through fluorescence reception optical fiber, two enter scene 2 optical fiber to visible spectral coverage spectrogrph
Carry out opto-electronic conversion and become spectroscopic data, as fluorescence spectrum is used spectral coverage visible with super continuous spectrums test
Sampling location and sampling number, i.e. N3=N1.N3 spectroscopic data warp of visible spectral coverage spectrogrph output
USB interface delivers to master control and data analysis system stores.
(5) data analysis and fusion
The super continuous spectrums laser of visible spectral coverage is overflow N1 spectroscopic data and the fluorescence spectrum of integrated spectral
N3 spectroscopic data is weighted superposition, obtains the fusion spectroscopic data of N1 visible spectral coverage.Wherein fluorescence
The weighted value of spectrum is F=0.7, and it is 1-F that super continuous spectrums laser overflows the weighted value of integrated spectral.
The super continuous spectrums laser merging spectroscopic data and infrared spectral coverage of visible for this N1 spectral coverage is overflow comprehensive light
N2 spectroscopic data of spectrum, N number of spectroscopic data is for subsequent analysis altogether.Based on PCA
(principal components analysis is called for short PCA) calculates M main constituent of this N number of spectroscopic data
Numerical value, carries out dimension-reduction treatment, and number of principal components M is taken as 7.
(6) blood differentiates and judges
By M main constituent numerical value of blood to be checked, obtain its characteristic vector in M dimension main constituent space,
This feature vector and M are tieed up the people obtained by this blood identifier and animal's whole blood, the blood in main constituent space
Slurry, serum M dimension main constituent spatial database cluster centre characteristic vector contrast, according to feature to
First amount similarity determines the type blood of blood to be checked, is i.e. whole blood, blood plasma or serum.Then, then
By M main constituent numerical value of blood to be checked, with the M dimension of the people under this type blood with animal different genera
The cluster centre characteristic vector of main constituent spatial database contrasts, and determines according to characteristic vector similarity again
Kind, is i.e. people or animal blood, if animal blood, is which kind of animal blood, so far, completes
The type blood of blood to be checked and the judgement of kind.
Then, master control and data analysis system are by the Ultra-Violet Laser induced fluorescence spectrum of blood to be checked and visible red
The curve of spectrum and the result of determination of outer super continuous unrestrained comprehensive laser spectrum show in touch screen man-machine interaction circle
On face, with for reference.So far whole test process is completed.
The invention has the beneficial effects as follows, Ultraluminescence spectral detection uses ultraviolet narrow linewidth laser, knot
Close fiber optic collimator and microcobjective focuses to blood sample in test tube, and use transmitting to receive coaxial optical fiber end face
To fluorescent scattering signal after design collection blood sample, it is effectively improved blood laser fluorescence spectrum signal in test tube
Signal to noise ratio;Use the integrating sphere of particular design, either blood plasma, the diffusing transmission of serum transparency liquid, go back
It is whole blood and the diffuse-reflectance of test tube label, and the unrestrained suction of whole test sample is penetrated and the unrestrained wide spectrum letter launched
Number all can receive, i.e. be applicable to all kinds of blood, all kinds of material test tube, all kinds of different anticoagulant and difference
Spectroscopic data under label condition uniformly gathers.Have employed visible to mid-infrared high power super continuous spectrums lasing light emitter
And the bispectrum section spectrogrph of correspondence, obtain wide spectrum and overflow comprehensive laser spectrum data, can thin to encapsulation blood
Micro-optic difference catches, and can improve discriminating accuracy;Hardware uses Y shape fiberoptic connection two to enter scene 2 light
Fine multiplexed optical spectrometer, software uses weighted superposition, it is achieved spectroscopic data information fusion.Due to hardware configuration
With the robustness of software analysis, the blood identifier of the present invention is applicable to the automatic of whole blood, blood plasma and serum
Identify.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the present invention, wherein (a) super continuous spectrums laser overflow integrated spectral test;(b) ultraviolet
Laser fluorescence spectrum is tested;(c) fibre profile 1 figure;(d) fibre profile 2 figure;(e) fibre profile 3 figure.
In figure: 1 power supply module;Spectral coverage spectrogrph seen from 2;3 infrared spectral coverage spectrogrphs;4—
Spectrogrph optical fiber interface;5 liang are entered scene 2 optical fiber;6 USB interface;7 master controls and data
Analysis system;8 integrating sphere optical fiber interfaces;9 integrating sphere joints;10 test tube mechanical hands;
11 connecting rods;12 guide rails;13 robot movement controllers;14 test tube caps;15—
Test tube;16 circular holes;17 integrating sphere sample wells;18 sample room outer cover plates;19 treat
Inspection blood;20 receive optical axis;21 sample rooms;22 base plates;23 integrating sphere right-hand parts;
24 diffuse-reflectance coatings;25 launch optical axis;26 integrating sphere left sides;27 light source chambers;
28 integrating sphere light source holes;29 supports;30 light source chamber outer cover plates;31 fiber optic collimators connect
Head;32 laser instrument tail optical fibers;33 touch screen human-computer interaction interfaces;34 super continuous spectrums laser instrument;
35 integrating spheres;36 handling main shafts;37 Ultra-Violet Laser optical axises;38 fluorescence spectrum tests
Point;39 objective lens supports;40 microcobjectives;41 object lens couplings;42 fiber optic collimators
Mirror;43 Y shape coaxial optical fibers;44 fiber optic connectors;45 ultraviolet emission optical fiber;46——
Fluorescence reception optical fiber;47 fluorescence reception optical fiber arrangements;48 ultraviolet emission optical fiber arrangements;49——
Ultraviolet narrow linewidth laser.
Detailed description of the invention
The hardware system sealing blood identifier of super continuous unrestrained comprehensive laser spectrum is combined based on Ultraluminescence
As shown in Figure 1, hardware system is mainly by power supply module 1, visible spectral coverage spectrogrph 2, infrared spectral coverage light for structure
Spectrometer 3, two enter scene 2 optical fiber 5, master control and data analysis system 7, integrating sphere 35, test tube mechanical hand 10,
Connecting rod 11, guide rail 12, robot movement controller 13, sample room outer cover plate 18, base plate 22, support
29, light source chamber outer cover plate 30, fiber optic collimator joint 31, laser instrument tail optical fiber 32, super continuous spectrums laser instrument 34,
Objective lens support 39, microcobjective 40, object lens coupling 41, fiber optic collimator mirror 42, Y shape coaxial optical fiber 43,
Fiber optic connector 44, ultraviolet narrow linewidth laser 49 form.
Wherein, integrating sphere 35 is made up of integrating sphere right-hand part 23 and 26 two, integrating sphere left side hemisphere,
They are coupled by integrating sphere joint 9, and are fixed on base plate 22.Integrating sphere 35 inwall is coated with
Cover diffuse-reflectance coating 24, the light being irradiated to inwall is played the effect of the even light of diffuse-reflectance.Integrating sphere right-hand part
23 have integrating sphere sample well 17, and sample room outer cover plate 18 is arranged on base plate 22 and integrating sphere right-hand part 23
On, formed and close Proteins room 21, to eliminate the impact of veiling glare.Open above sample room outer cover plate 18
Have a circular hole 16, handling main shaft 36 through integrating sphere sample well 17 and the center of circular hole 16, and with base plate 22
Vertically.Integrating sphere left side 26 has integrating sphere light source hole 28 and integrating sphere optical fiber interface 8 (note: this enforcement
Example is SMA905 interface).
Y shape coaxial optical fiber 43 is made up of with fluorescence reception optical fiber 46 ultraviolet emission optical fiber 45, and both converge
Cheng Yigen optical fiber, its fiber end face is coaxial distribution, and central circular is ultraviolet emission optical fiber arrangement 48,
Outer concentric circular ring region territory is fluorescence reception optical fiber arrangement 47, and this geometric configuration can swash by efficient reception Ultra-Violet Laser
The backward fluorescent scattering signal sent out.Y shape coaxial optical fiber 43 couples with fiber optic collimator mirror 42 and can realize collimation
Penetrating and receive, fiber optic collimator mirror 42 is connected with microcobjective 40 by object lens coupling 41, can be by purple
Outer laser beam focus enters to fluorescence spectrum test point 38 and to the backscatter signal of fluorescence spectrum test point 38
Row is collected.Microcobjective 40 is fixedly installed on base plate 22 by objective lens support 39.
Visible spectral coverage spectrogrph 2 and infrared spectral coverage spectrogrph 3 all use same light spectrometer optical fiber interface 4 (note:
The present embodiment is SMA905 interface), two enter scene 2 optical fiber 5 is divided into two receiving terminals (i.e. two enter) and two
Outfan (i.e. scene 2).One receiving terminal couples with integrating sphere optical fiber interface 8, can collect from receiving optical axis
The diffuse-reflectance light of 20;Another receiving terminal is coupled by fiber optic connector 44 with fluorescence reception optical fiber 46
(note: be SMA905 coupling in the present embodiment), can collect the fluorescence from fluorescence reception optical fiber 46 and believe
Number;Two outfans couple with visible spectral coverage spectrogrph 2 and infrared spectral coverage spectrogrph 3 respectively.
Super continuous spectrums laser instrument 34 (note: its spectral region 400nm-2400nm in the present embodiment, power 1W,
Repetition 1MHz, pulsewidth 150ps) the visible and infrared spectral coverage super continuous spectrums pulse laser that sends is through laser tail
Fine 32 transmission, export super continuous spectrums pulse after then carrying out beam collimation by fiber optic collimator joint 31 and collimate
Laser beam, and enter integrating sphere 35 along launching optical axis 25 through integrating sphere light source hole 28.
Fiber optic collimator joint 31 is fixed on base plate 22 by support 29, and by light source chamber outer cover plate 30 with
Integrating sphere left side 26 shell couples, and is formed and closes spatial light source chamber 27, to eliminate the impact of veiling glare.
The ultraviolet continuous laser beam that ultraviolet narrow linewidth laser 49 sends ultraviolet in Y shape coaxial optical fiber 43
Launching fiber 45 transmits, launch tailing edge Ultra-Violet Laser optical axis 37 from ultraviolet emission optical fiber arrangement 48 transmits,
Collimate through fiber optic collimator mirror 42, microcobjective 40 can realize the purple to fluorescence spectrum test point 38 after focusing on
Outer induced fluorescence excites (note: Ultra-Violet Laser optical axis 37 intersects, and its intersection point is fluorescence light with handling main shaft 36
Spectrum test point 38), the back scattering fluorescence signal of fluorescence spectrum test point 38 successively through microcobjective 40 and
By the outer concentric circular ring region territory of Y shape coaxial optical fiber 43 end face, i.e. fluorescence reception light after fiber optic collimator mirror 42
Fine arrangement 47 collection, then enter scene 2 optical fiber 5 to visible spectral coverage spectrogrph 2 through fluorescence reception optical fiber 46, two
It is received and analyzes.
Guide rail 12 and base plate 22 right angle setting, test tube mechanical hand 10 is by connecting rod 11 and guide rail 12
Connect and can slide along guide rail 12 under robot movement controller 13 controls.Blood to be checked 19 is by test tube cap
14 are encapsulated in test tube 15.Test tube mechanical hand 10, under robot movement controller 13 controls, can be firmly grasped
Test tube cap 14 also drives test tube 15 to move up and down along handling main shaft 36 direction.
Power supply module 1 is in order to divide visible spectral coverage spectrogrph 2, infrared spectral coverage spectrogrph 3, master control and data
Analysis system 7, super continuous spectrums laser instrument 34, ultraviolet narrow linewidth laser 49 and robot movement controller 13
It is powered.Master control and data analysis system 7 are in order to visible spectral coverage spectrogrph 2, infrared spectral coverage spectrogrph
3, super continuous spectrums laser instrument 34, ultraviolet narrow linewidth laser 49 and robot movement controller 13 are controlled
System, and receive visible spectral coverage spectrogrph 2 and the light of infrared spectral coverage spectrogrph 3 output by USB interface 6
Modal data processes and analyzes.Master control and data analysis system 7 included touch screen human-computer interaction interface 33,
For the man-machine interaction with user, accept the instruction of user and export result to user.
The hemanalysis sealing blood identifier of super continuous unrestrained comprehensive laser spectrum is combined based on Ultraluminescence
Method the steps include:
(1) instrument starts and super continuous spectrums test sample introduction
Visible spectral coverage spectrogrph 2, infrared spectral coverage spectrogrph 3, master control and data are divided by power-on module 1
Analysis system 7, super continuous spectrums laser instrument 34, ultraviolet narrow linewidth laser 49 and robot movement controller 13
It is powered.
User starts test mastery routine by touch screen human-computer interaction interface 33.Now, master control and data are divided
Analysis system 7 sends sample introduction and instructs to robot movement controller 13, and robot movement controller 13 controls examination
Pipe mechanical hand 10 captures test tube 15 sample introduction and enters integrating sphere 35 to it along handling main shaft 36 direction.Test tube 15
Bottom position higher than launch optical axis 25 and with receive optical axis 20 non-intersect, it is to avoid laser beam direct irradiation is to be checked
Test tube 15, and avoid the transflector light of test tube 15 directly transmit along transmitting optical axis 25 and entered scene 2 light by two
Fine 5 collect.
(2) super continuous spectrums laser overflow integrated spectral test
As shown in Figure 1a, master control and data analysis system 7 send instruction start super continuous spectrums laser instrument 34,
Visible spectral coverage spectrogrph 2 and infrared spectral coverage spectrogrph 3.The super continuous spectrums of super continuous spectrums laser instrument 34 output is accurate
Collimated excitation beam enters integrating sphere 35 along launching optical axis 25, and after laser beam irradiation to diffuse-reflectance coating 24, it is anti-
Penetrating light is diffuse-reflectance, i.e. transmits along all directions in integrating sphere 35, becomes uniform light.Test tube 15 is subject to
After the super continuous spectrums light of different directions irradiates, test tube 15 (note: include its material and label outward) is with to be checked
The diffuse-reflectance of blood 19, diffusing transmission, absorb, launch after light will transmit along space any direction, meet
To diffuse-reflectance coating 24 diffuse-reflectance to any direction, the light in integrating sphere 35 is transmitted to have and overflows comprehensively by it
Spectrum affects.
Being entered after scene 2 optical fiber 5 collects along launching the diffuse-reflectance light of optical axis 25 transmission by two, delivering to respectively can
See that spectral coverage spectrogrph 2 and infrared spectral coverage spectrogrph 3 carry out opto-electronic conversion and become spectroscopic data.Originally it is embodied as
In example, the spectral region of visible spectral coverage spectrogrph 2 is 370-850nm, and sampled point is N1=1300.Ultrared spectrum
The spectral region of section spectrogrph 3 is 855-1744nm, and sampled point is N2=512.Visible spectral coverage spectrogrph 2
And common N=N1+N2 the spectroscopic data of infrared spectral coverage spectrogrph 3 output through USB interface 6 deliver to master control and
Data analysis system 7 stores.
(3) fluorescence spectrum test sample introduction
Master control and data analysis system 7 send out sample and instruct to robot movement controller 13, robot movement
Controller 13 controls test tube mechanical hand 10 and drives test tube 15 to it along handling main shaft 36 direction removal integrating sphere
35 and sample room 21, the center of test tube 15 blood to be checked 19 it is positioned to fluorescence spectrum test point 38,
Now complete fluorescence spectrum test sample introduction.
(4) Ultra-Violet Laser fluorescence spectrum test
As shown in Figure 1 b, master control and data analysis system 7 send instruction start ultraviolet narrow linewidth laser 49,
Visible spectral coverage spectrogrph 2 and infrared spectral coverage spectrogrph 3.Ultraviolet narrow linewidth laser 49 (note: the present embodiment
Middle employing wavelength is 360nm ± 1nm, the semiconductor pumped solid continuous wave laser of power 0.05W) send
Ultraviolet continuous laser beam collimated focusing post-concentration is to the blood to be checked 19 at fluorescence spectrum test point 38, purple
The fluorescence spectrum signal that outer narrow linewidth continuous laser is induced is successively through microcobjective 40 and fiber optic collimator mirror 42
After by the outer concentric circular ring region territory of Y shape coaxial optical fiber 43 end face, i.e. fluorescence reception optical fiber arrangement 47 collection,
Enter scene 2 optical fiber 5 through fluorescence reception optical fiber 46, two again to carry out opto-electronic conversion to visible spectral coverage spectrogrph 2 and become
Spectroscopic data, in this specific embodiment, the spectral region of visible spectral coverage spectrogrph 2 is 370-850nm, to glimmering
Light spectrum uses the sampling location as testing visible spectral coverage with super continuous spectrums and sampling number, i.e.
N3=N1=1300.Owing to the wavelength of ultraviolet narrow linewidth laser 49 is positioned at the spectrum of visible spectral coverage spectrogrph 2
Outside scope, therefore Ultra-Violet Laser echo does not interferes with the collection to fluorescence spectrum signal, it is not necessary to use Rayleigh
Optical filter suppression echo interference.N3 spectroscopic data of visible spectral coverage spectrogrph 2 output is through USB interface 6
Deliver to master control and data analysis system 7 stores.
(5) data analysis and fusion
The super continuous spectrums laser of visible spectral coverage is overflow N1 spectroscopic data and the fluorescence spectrum of integrated spectral
N3 spectroscopic data is weighted superposition, obtains the fusion spectroscopic data of N1 visible spectral coverage.Wherein fluorescence
The weighted value of spectrum is F, and it is 1-F that super continuous spectrums laser overflows the weighted value of integrated spectral.(note: the present embodiment
Middle F=0.7)
The super continuous spectrums laser merging spectroscopic data and infrared spectral coverage of visible for this N1 spectral coverage is overflow comprehensive light
N2 spectroscopic data of spectrum, N number of spectroscopic data is for subsequent analysis altogether.Based on PCA
(principal components analysis is called for short PCA) calculates M main constituent of this N number of spectroscopic data
Numerical value (note: M=7 in the present embodiment i.e. calculates 7 main constituent numerical value), carries out dimension-reduction treatment.
(6) blood differentiates and judges
By M main constituent numerical value of blood 19 to be checked, obtain its feature in M dimension main constituent space to
Amount, by the people and the animal's whole blood that are obtained by this blood identifier in this feature vector and M dimension main constituent space,
The cluster centre characteristic vector of blood plasma, serum M dimension main constituent spatial database contrasts, according to feature
First vector similarity determines the type blood of blood 19 to be checked, is i.e. whole blood, blood plasma or serum.So
After, then by M main constituent numerical value of blood 19 to be checked, the most of the same race with animal with the people under this type blood
The cluster centre characteristic vector of the M dimension main constituent spatial database belonged to contrasts, according to characteristic vector phase
Determine kind again like degree, be i.e. people or animal blood, if animal blood, be which kind of animal blood,
So far, the type blood of blood 19 to be checked and the judgement of kind are completed.
Then, master control and data analysis system 7 by the Ultra-Violet Laser induced fluorescence spectrum of blood 19 to be checked and
Visible infrared excess overflows the curve of spectrum of comprehensive laser spectrum continuously and result of determination shows at touch screen man-machine
On interactive interface 33, with for reference.So far whole test process is completed.
Claims (1)
1. a sealing blood discriminating instrument for super continuous unrestrained comprehensive laser spectrum is combined based on Ultraluminescence,
It includes power supply module (1), visible spectral coverage spectrogrph (2), infrared spectral coverage spectrogrph (3), two enters scene 2
Optical fiber (5), master control and data analysis system (7), integrating sphere (35), test tube mechanical hand (10), connection
Bar (11), guide rail (12), robot movement controller (13), sample room outer cover plate (18), base plate (22),
Support (29), light source chamber outer cover plate (30), fiber optic collimator joint (31), laser instrument tail optical fiber (32), super
Continuous spectrum laser instrument (34), objective lens support (39), microcobjective (40), object lens coupling (41), light
Fine collimating mirror (42), Y shape coaxial optical fiber (43), fiber optic connector (44), ultraviolet narrow linewidth laser
(49), it is characterised in that:
Described integrating sphere (35) is by integrating sphere right-hand part (23) and integrating sphere left side (26) two and half
Set of balls becomes, and they are coupled by integrating sphere joint (9), and are fixed on base plate (22);
Integrating sphere (35) inwall coating diffuse-reflectance coating (24), plays the even light of diffuse-reflectance to the light being irradiated to inwall
Effect, integrating sphere right-hand part (23) has integrating sphere sample well (17), sample room outer cover plate (18)
It is arranged on base plate (22) and integrating sphere right-hand part (23), is formed and close Proteins room (21), with
Eliminate the impact of veiling glare;Sample room outer cover plate (18) top has circular hole (16), handling main shaft (36)
Through integrating sphere sample well (17) and the center of circular hole (16) and vertical with base plate (22).Integrating sphere
Left side (26) has integrating sphere light source hole (28) and integrating sphere optical fiber interface (8);
Described Y shape coaxial optical fiber (43) is by ultraviolet emission optical fiber (45) and fluorescence reception optical fiber (46)
Forming, both pool an optical fiber, and its fiber end face is coaxial distribution, and central circular is that ultraviolet is sent out
Penetrating optical fiber arrangement (48), outer concentric circular ring region territory is that fluorescence reception optical fiber arranges (47), this geometric configuration
Can the backward fluorescent scattering signal that excites of efficient reception Ultra-Violet Laser;Y shape coaxial optical fiber (43) is accurate with optical fiber
Straight mirror (42) coupling can realize collimation and launches and receive, and fiber optic collimator mirror (42) passes through object lens coupling (41)
It is connected with microcobjective (40), UV laser beam can be focused to fluorescence spectrum test point (38) right
The backscatter signal of fluorescence spectrum test point (38) is collected;Objective lens support (39) is by microcobjective
(40) it is fixedly installed on base plate (22);
Described visible spectral coverage spectrogrph (2) and infrared spectral coverage spectrogrph (3) all use same light spectrometer light
Fine interface (4), two enter scene 2 optical fiber (5) is divided into two receiving terminals and two outfans, a receiving terminal
Couple with integrating sphere optical fiber interface (8), can collect from the diffuse-reflectance light receiving optical axis (20);Separately
One receiving terminal is coupled by fiber optic connector (44) with fluorescence reception optical fiber (46), can collect from glimmering
The fluorescence signal of light-receiving optical fiber (46);Two outfans respectively with visible spectral coverage spectrogrph (2) and red
Outer spectral coverage spectrogrph (3) couples;
Visible and the infrared spectral coverage super continuous spectrums pulse laser warp that described super continuous spectrums laser instrument (34) sends
Laser instrument tail optical fiber (32) transmits, and after then carrying out beam collimation by fiber optic collimator joint (31), output is super
Continuous spectrum pulse collimated laser beam, and enter long-pending through integrating sphere light source hole (28) along launching optical axis (25)
Bulb separation (35);
Described fiber optic collimator joint (31) is fixed on base plate (22) by support (29), and passes through light
Source chamber outer cover plate (30) couples with integrating sphere left side (26) shell, is formed and closes spatial light source chamber (27),
To eliminate the impact of veiling glare;
The wavelength of described ultraviolet narrow linewidth laser (49) is positioned at the spectrum of visible spectral coverage spectrogrph (2)
Outside scope, the ultraviolet continuous laser beam that it sends ultraviolet emission optical fiber in Y shape coaxial optical fiber (43)
(45) transmission, from ultraviolet emission optical fiber arrangement (48) launch tailing edge Ultra-Violet Laser optical axis (37) transmit,
Can realize fluorescence spectrum test point (38) after fiber optic collimator mirror (42) collimation, microcobjective (40) focus on
Ultraviolet induced fluorescence excite.Ultra-Violet Laser optical axis (37) intersects with handling main shaft (36), and its intersection point is
Fluorescence spectrum test point (38), the back scattering fluorescence signal of fluorescence spectrum test point (38) is successively through aobvious
By the outer concentric circular of Y shape coaxial optical fiber (43) end face after speck mirror (40) and fiber optic collimator mirror (42)
Ring region territory, i.e. fluorescence reception optical fiber arrangement (47) are collected, then through fluorescence reception optical fiber (46), two enter two
Go out optical fiber (5) to visible spectral coverage spectrogrph (2) be received and analyze;
Described guide rail (12) and base plate (22) right angle setting, test tube mechanical hand (10) passes through connecting rod
(11) couple with guide rail (12) and can be along guide rail (12) under robot movement controller (13) controls
Slide.Blood to be checked (19) is encapsulated in test tube (15) by test tube cap (14).Test tube mechanical hand (10)
Under robot movement controller (13) controls, test tube cap (14) can be firmly grasped and drive test tube (15) to exist
Handling main shaft (36) direction moves up and down;
Described power supply module (1) in order to visible spectral coverage spectrogrph (2), infrared spectral coverage spectrogrph (3),
Master control and data analysis system (7), super continuous spectrums laser instrument (34), ultraviolet narrow linewidth laser (49)
And robot movement controller (13) is powered.Master control and data analysis system (7) are in order to visible
Spectral coverage spectrogrph (2), infrared spectral coverage spectrogrph (3), super continuous spectrums laser instrument (34), ultraviolet narrow linewidth
Laser instrument (49) and robot movement controller (13) are controlled, and are connect by USB interface (6)
The spectroscopic data that spectral coverage spectrogrph (2) and infrared spectral coverage spectrogrph (3) seen from receiving export carries out processing and dividing
Analysis.Master control and data analysis system (7) included touch screen human-computer interaction interface (33), for user's
Man-machine interaction, accepts the instruction of user and exports result to user;
Master control and data analysis system send instruction and start super continuous spectrums laser instrument, visible spectral coverage spectrogrph and red
Outer spectral coverage spectrogrph, the super continuous spectrums collimated laser beam of super continuous spectrums laser instrument output enters long-pending along launching optical axis
Bulb separation, after laser beam irradiation to diffuse-reflectance coating, its reflection light is diffuse-reflectance, and test tube is surpassed by different directions
Continuous spectrum light irradiate after, the diffuse-reflectance with blood to be checked of labelling outside test tube, test tube, diffusing transmission, absorption,
Light after transmitting will transmit along space any direction, run into diffuse-reflectance coating diffuse-reflectance to any direction, its
Light in integrating sphere is transmitted there is the impact of unrestrained integrated spectral;Along the diffuse-reflectance light quilt launching optical axis transmission
After two enter the collection of scene 2 optical fiber, deliver to visible spectral coverage spectrogrph respectively and infrared spectral coverage spectrogrph carries out photoelectricity and turns
Transformation becomes spectroscopic data;Master control and data analysis system send instruction and start ultraviolet narrow linewidth laser, visible
Spectral coverage spectrogrph and infrared spectral coverage spectrogrph;The ultraviolet continuous laser beam that ultraviolet narrow linewidth laser sends is through standard
The straight post-concentration that focuses on is to the blood to be checked at fluorescence spectrum test point, and ultraviolet narrow linewidth continuous laser is induced
Fluorescence spectrum signal is same by the outer ring of Y shape coaxial optical fiber end face after microcobjective and fiber optic collimator mirror successively
Heart circle ring area, i.e. fluorescence reception optical fiber arrangement collects, then through fluorescence reception optical fiber, two enter scene 2 optical fiber extremely
Visible spectral coverage spectrogrph carries out opto-electronic conversion and becomes spectroscopic data;Visible spectral coverage spectrogrph and infrared spectral coverage spectrum
The spectroscopic data of instrument output delivers to master control and data analysis system carries out storing, analyzing and processing through USB interface.
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