CN105838594B - A kind of microculture tank arrangement - Google Patents
A kind of microculture tank arrangement Download PDFInfo
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- CN105838594B CN105838594B CN201610321215.2A CN201610321215A CN105838594B CN 105838594 B CN105838594 B CN 105838594B CN 201610321215 A CN201610321215 A CN 201610321215A CN 105838594 B CN105838594 B CN 105838594B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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Abstract
A kind of microculture tank arrangement, belongs to biological fermentation equipment, solves the problems, such as that the pH value control that existing culture tank arrangement is present is uneven, to optimize nertralizer addition manner, improve fermentation production efficiency.The present invention includes culture tank, nertralizer storage tank, circulating pump and programmable logic controller (PLC)；The culture tank includes tank body, multiple agitators and nertralizer distributor, tank body top has air inlet pipe, escaping pipe, feed pipe and respirator, tank base has drainage conduit, the multiple agitator is distributed in tank interior along tank body axis, multiple nertralizer distributors are distributed in tank interior along tank body axis, and arranged with the multiple agitator transpostion interval, each nertralizer distributor is the closure circle being made up of stainless steel tube, and circle bottom surface has equally distributed aperture.It is easy to manufacture of the present invention, easy to use, improve nertralizer and add precision, it is to avoid pipeline scale is blocked, it is ensured that pH value fluctuates in controlled range in tank, improves fermenting and producing level.
Description
Technical field
The invention belongs to biological fermentation equipment, and in particular to a kind of microculture tank arrangement.
Background technology
Including many staple products including all feeds, food additives and bulk drug, mainly by fermenting and producing
Obtain.The capital equipment of fermenting and producing is culture tank arrangement.In most fermentation production process, microbes have been secreted out of
Machine acid or alkaline metabolin, cause zymotic fluid pH value that fluctuation is presented, and need to supplement nertralizer in good time in fermentation production process, to maintain
Suitable fermentation acid-base value environment.
Existing culture tank arrangement, mainly including tank body, agitator, temperature control tubulation, air inlet pipe, escaping pipe, feed pipe, discharge
Pipe, temperature electrode, pH electrodes and dissolved oxygen electrode.Existing culture tank is typically added liquid or powder nertralizer by single pipe
Culture tank, because culture tank is often bulky, causes nertralizer rapidly to be spread out in culture tank, causes local pH
Value is too high or too low, is unfavorable for the carrying out of microbial fermentation.In addition some solid nertralizers addition is difficult, and is easily caused pipeline and blocks up
Plug, is also unfavorable for the carrying out of production.By taking lactic fermentation production as an example, majority producer uses calcium hydroxide as nertralizer at present,
And calcium hydroxide has two kinds of addition manners, one kind is added with suspension form stream, and another kind is added with solidapowder form.With
Suspension form is added to be had the easy fouling of calcium hydroxide, blocks pipeline；And being added with solidapowder form to cause
The delay and uneven of pH value control, pH value is difficult to control.
The content of the invention
The present invention provides a kind of microculture tank arrangement, and the pH value control solved existing for existing culture tank arrangement is uneven
Problem, with optimize nertralizer addition manner, improve fermentation production efficiency.
A kind of microculture tank arrangement provided by the present invention, including culture tank, nertralizer storage tank, circulating pump and can compile
Journey logic controller (PLC)；The culture tank includes tank body and multiple agitators, and the tank body has temperature regulation structure, uses
In the temperature of regulation tank body；The multiple agitator tank interior along tank body axis be distributed, tank body top have air inlet pipe,
Escaping pipe, feed pipe, tank base have drainage conduit, the side wall insertion pH electrodes of tank body；The nertralizer tank bottom passes through
Pipeline is connected with pump inlet, and circulating-pump outlet connects control valve one end and nertralizer feed pipe respectively by pipeline, in
The top of the nertralizer storage tank is connected with agent feed pipe；The pH electrodes connect programmable logic controller (PLC) by wire
(PLC) input, the output end of programmable logic controller (PLC) (PLC) connects the control end of control valve by wire；It is special
Levy and be：
It also includes respirator and multiple nertralizer distributors, and the respirator is millipore filter, loaded on tank body top
Portion, tank body is communicated with air during for charging and discharge；
The control valve other end connects multiple nertralizer distributors respectively by pipeline, and the nertralizer distributor is
The closure circle being made up of stainless steel tube, circle bottom surface has equally distributed aperture, and multiple nertralizer distributors are in tank body
Portion is distributed along tank body axis, and is arranged with the multiple agitator transpostion interval, flat where the circle of each nertralizer distributor
Face is vertical with tank body axis.
Described microculture tank arrangement, it is characterised in that：
The temperature regulation structure of the tank body is the side wall that temperature control tubulation or tank body are evenly distributed with along the inwall of tank body
With circular hollow interlayer, temperature control tubulation and hollow interlayer are used to be passed through thermal medium or cold medium, tank body is heated up or is lowered the temperature；
It is the 1/4~3/4 of tank diameter, the internal diameter of stainless steel tube to constitute the closed circle loop diameter of the nertralizer distributor
It is 0.5cm~30cm.
Described microculture tank arrangement, it is characterised in that：
The nertralizer storage tank is 2, and 2 nertralizer storage tanks are in parallel, are respectively used in deposit acid acceptor and alkalescence
And agent.
PH electrodes measure zymotic fluid pH value in real time positioned at the suitable position of tank body, and provide data to Programmable logical controller
Device (PLC)；Programmable logic controller (PLC) (PLC) is connected by data wire with remote monitoring center, is carried out data processing and is controlled
The switch of control valve processed；Nertralizer storage tank, tank body uses firm corrosion resistant material, such as stainless steel or plastic material,
In case nertralizer burn into is revealed；Circulating pump needs selection according to actual process, in nertralizer is pressed into culture tank and is made
Precipitation is prevented with agent flowing.
After fermenting and producing starts, start circulating pump, make nertralizer in pipeline internal circulation flow, as fermentation is carried out, hair
The pH value of zymotic fluid changes, the robot control system(RCS) being made up of pH electrodes, programmable logic controller (PLC) (PLC) and control valve, control
The pH value of seed or zymotic fluid is setup parameter, and according to the pH value parameter of setting, control valve is turned on and off, in culture tank
Nertralizer is pumped into, to neutralize the organic acid or alkaline matter of continuous generation, realizes that zymotic fluid pH value is automatically adjusted to setup parameter.
The fermenting and producing product of many large-tonnages relies primarily on yield and wins victory, and process units and production technology only accomplish essence
Refinement could will reduce production cost, produce preferable economic benefit.The present invention sets the distribution of multilayer nertralizer in culture tank
Device, can uniformly send nertralizer into each position in tank at any time by circulating system, improve nertralizer and add precision, keep away
Exempt from the nertralizers such as calcium hydroxide and be converted into calcium carbonate and in the duct fouling and clogging pipeline, it is ensured that pH value is in controlled range in tank
Fluctuation, improves fermenting and producing level, it is adaptable to use the unicellular microorganisms such as bacterium and yeast to produce the fermenting and producing of bacterium.
Brief description of the drawings
Fig. 1 is installation for fermenting composition structural representation of the present invention.
Specific embodiment
Below in conjunction with drawings and Examples, the present invention is further described.
As shown in figure 1, the present invention includes culture tank 100, nertralizer storage tank 200, circulating pump 300 and Programmable logical controller
Device (PLC) 400；The culture tank 100 includes tank body 110 and multiple agitators 130, and the tank body 110 has temperature adjustment knot
Structure, the temperature for adjusting tank body 110；The multiple agitator 130 is distributed inside tank body 110 along tank body axis, tank body
110 tops have air inlet pipe 111, escaping pipe 112, feed pipe 113, and the bottom of tank body 110 has drainage conduit 114, the side of tank body 110
Wall insertion pH electrodes 410；The bottom of nertralizer storage tank 200 is connected by pipeline with the import of circulating pump 300, and circulating pump 300 goes out
Mouthful the one end of control valve 310 and nertralizer feed pipe 320 are connected by pipeline respectively, nertralizer feed pipe 320 connect it is described in
With the top of agent storage tank 200；The pH electrodes 410 connect the input of programmable logic controller (PLC) (PLC) 400 by wire,
The output end of programmable logic controller (PLC) (PLC) 400 connects the control end of control valve 310 by wire；
It also includes respirator 140 and multiple nertralizer distributors 150, and the respirator is millipore filter, is loaded on
The top of tank body 110, tank body 110 is communicated with air during for charging and discharge；
The other end of the control valve 310 connects multiple nertralizer distributors 150, the nertralizer point respectively by pipeline
Cloth device 150 is the closure circle being made up of stainless steel tube, and circle bottom surface has equally distributed aperture, multiple nertralizer distributors
150 are distributed inside tank body 110 along tank body axis, and are arranged with the transpostion interval of the multiple agitator 130, each nertralizer point
Plane where the circle of cloth device is vertical with tank body axis.
Embodiment 1, tank volume 500m3, the inwall of the tank body 110 is evenly distributed with temperature control tubulation 120, for being passed through
Thermal medium or cold medium, heat up or lower the temperature to tank body, and respirator 140 is 0.2 μm of millipore filter；
Nertralizer storage tank 200 is 1, lays in antalkali；Constitute the closed circle loop diameter of the nertralizer distributor
It is the 1/4 of the diameter of tank body 110, the internal diameter of stainless steel tube is 30cm.
Lactic fermentation production is carried out using lactic acid bacteria using the device, using high temperature resistant anaerobism lactic acid bacteria (cheese breast
Acidfast bacilli Lactobacillus casei B9232) carry out fermented and cultured.Gone out being added after culture tank steam sterilizing disinfecting
The culture medium of bacterium, is controlled at 40 DEG C culture medium temperature using temperature control tubulation；Seed is added in culture tank 100 again, in
With the calcium hydroxide suspension that sterilization is added in agent storage tank 200, circulating pump 300 is opened, calcium hydroxide suspension is existed
The internal circulation flow of nertralizer feed pipe 320 so as not to sedimentation fouling.Using programmable logic controller (PLC) (PLC) 400 according to pH electrodes
410 detection datas, the addition of nertralizer calcium hydroxide is controlled by control valve 310, finally makes the pH during fermented and cultured
Value stabilization is 6.5.About 37 hours or so, fermentation ends put tank, put tank lactic acid concn 178g/L, and fermentation time reduction, lactic acid is produced
Amount is improved.
Embodiment 2, tank volume 8L, the side wall of the tank body 110 has circular hollow interlayer, for being passed through thermal medium
Or cold medium, tank body being heated up or is lowered the temperature, respirator 140 is 0.2 μm of millipore filter；
Nertralizer storage tank 200 is 2, and 2 nertralizer storage tanks 200 are in parallel, is respectively used to lay in acid acceptor and alkalescence
Nertralizer；It is the 3/4 of the diameter of tank body 110 to constitute the closed circle loop diameter of the nertralizer distributor, and the internal diameter of stainless steel tube is
0.5cm。
Fermenting and producing active dry yeasr mycelium is carried out using saccharomycete using the device, sugar-free yeast strain (bread is used
Yeast Saccharomyces cerevisiae CICC 1525) carry out fermented and cultured.After culture tank steam sterilizing disinfecting
Sterilized culture medium is added, culture medium temperature is controlled at 32 DEG C using hollow interlayer；Seed is added to culture tank 100 again
In, the feed-batch culture by the way of stream sugaring honey.In two sets of nertralizer storage tanks 200 in parallel, it is separately added into sterilized
30% sulfuric acid and 30% sodium carbonate liquor.In earlier fermentation, zymotic fluid pH value has downward trend, opens circulating pump 300, first
Make 30% sodium carbonate liquor in the internal circulation flow of nertralizer feed pipe 320, using programmable logic controller (PLC) (PLC) 400 according to
According to the detection data of pH electrodes 410, the addition of nertralizer sodium carbonate liquor is controlled by control valve 310, make fermented and cultured process
In pH stable 4.3；Fermentation later stage, zymotic fluid pH value is on the rise, by sodium carbonate liquor in nertralizer feed pipe 320
Discharge, 30% sulfuric acid is added in nertralizer feed pipe 320, makes the pH stable during fermented and cultured 4.3.About 20
Hour or so, zymotic fluid weight in wet base reaches 200g/L, and the fermentative activity hit the target 650 of yeast terminates fermentation and puts tank, ferments
Time shortens, yeast output increased.
Claims (3)
1. a kind of microculture tank arrangement, including culture tank (100), nertralizer storage tank (200), circulating pump (300) and can compile
Journey logic controller (400)；The culture tank (100) includes tank body (110) and multiple agitators (130), the tank body (110)
With temperature regulation structure, the temperature for adjusting tank body (110)；The multiple agitator (130) is on the internal edge of tank body (110)
Tank body axis is distributed, and tank body (110) top has air inlet pipe (111), escaping pipe (112), feed pipe (113), tank body (110)
The side wall that bottom has drainage conduit (114), tank body (110) inserts pH electrodes (410)；Nertralizer storage tank (200) bottom leads to
Piping is connected with circulating pump (300) import, and circulating pump (300) outlet connects control valve (310) one end respectively by pipeline
With nertralizer feed pipe (320), the top of nertralizer feed pipe (320) the connection nertralizer storage tank (200)；The pH electrodes
(410) input of programmable logic controller (PLC) (400), the output end of programmable logic controller (PLC) (400) are connected by wire
The control end of control valve (310) is connected by wire；It is characterized in that：
It also includes respirator (140) and multiple nertralizers distributor (150), and the respirator is millipore filter, is loaded on
Tank body (110) top, tank body (110) is communicated with air during for charging and discharge；
Control valve (310) other end connects multiple nertralizer distributors (150), the nertralizer point respectively by pipeline
Cloth device (150) is the closure circle being made up of stainless steel tube, and circle bottom surface has equally distributed aperture, multiple nertralizer distributions
Device (150) is internal along the distribution of tank body axis in tank body (110), and is arranged with the multiple agitator (130) transpostion interval, respectively
Plane where the circle of nertralizer distributor is vertical with tank body axis.
2. microculture tank arrangement as claimed in claim 1, it is characterised in that：
The temperature regulation structure of the tank body (110) is to be evenly distributed with temperature control tubulation (120) or tank body along the inwall of tank body
Side wall there is circular hollow interlayer, temperature control tubulation or hollow interlayer are used to be passed through thermal medium or cold medium, and tank body is heated up
Or cooling；
It is the 1/4~3/4 of tank body (110) diameter to constitute the closed circle loop diameter of the nertralizer distributor, stainless steel tube it is interior
Footpath is 0.5cm~30cm.
3. microculture tank arrangement as claimed in claim 1 or 2, it is characterised in that：
The nertralizer storage tank (200) is 2, and 2 nertralizer storage tanks (200) are in parallel, be respectively used to deposit acid acceptor and
Antalkali.
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| CN205616892U (en) * | 2016-05-16 | 2016-10-05 | 湖北广济药业股份有限公司 | Microbial cultivation canning is put |
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| CN101153296B (en) * | 2007-09-19 | 2014-06-18 | 北京化工大学 | Method for producing L-lactic acid by feedback control of substrate concentration with neutralizer |
| CN201390742Y (en) * | 2009-04-16 | 2010-01-27 | 河南科技大学 | Microorganism culture tank and microorganism culture apparatus using microorganism culture tank |
| CN103789194B (en) * | 2013-10-12 | 2016-03-16 | 云南龙谷生物科技有限公司 | A kind of microorganism culturing tank |
| CN204097451U (en) * | 2014-07-25 | 2015-01-14 | 上海瑞派机械有限公司 | Multifunctional fermentation tank |
| CN104403925A (en) * | 2014-12-21 | 2015-03-11 | 竹溪县宝田生物制品有限公司 | Microbe culture tank |
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