CN105838594A - Microorganism culture tank device - Google Patents
Microorganism culture tank device Download PDFInfo
- Publication number
- CN105838594A CN105838594A CN201610321215.2A CN201610321215A CN105838594A CN 105838594 A CN105838594 A CN 105838594A CN 201610321215 A CN201610321215 A CN 201610321215A CN 105838594 A CN105838594 A CN 105838594A
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- CN
- China
- Prior art keywords
- tank body
- tank
- nertralizer
- neutralizing agent
- distributor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
Abstract
The invention provides a microorganism culture tank device, and belongs to biological fermentation equipment. The problem that for an existing culture tank device, the pH value is not controlled evenly is solved, so that the neutralizing agent adding mode is optimized, and the fermentation production efficiency is improved. The microorganism culture tank device comprises a culture tank, a neutralizing agent storage tank, a circulating pump and a programmable logic controller; the culture tank comprises a tank body, multiple stirrers and multiple neutralizing agent distributors, the top of the tank body is provided with an air inlet tube, an air outlet tube, a feeding tube and a respirator, the bottom of the tank body is provided with a discharging tube, the stirrers are distributed inside the tank body along the central axis of the tank body, the neutralizing agent distributors are distributed inside the tank body along the central axis of the tank body, the stirrers and the neutralizing agent distributors are arranged in a crossed and alternate mode, each neutralizing agent distributor is a closed circle composed of a stainless steel tube, and small holes are uniformly distributed in the bottom face of each circle. The microorganism culture tank device is easy to manufacture and convenient to use, the precision of addition of a neutralizing agent is improved, scaling and plugging of a pipeline are avoided, it is guaranteed that the pH value in the tank fluctuates within a controllable range, and the fermentation production level is improved.
Description
Technical field
The invention belongs to biological fermentation equipment, be specifically related to a kind of microorganism culturing tank arrangement.
Background technology
Many staple products including all feeds, food additive and crude drug, main by sending out
Ferment produces and obtains.The capital equipment of fermenting and producing is culture tank device.In most fermentation production process,
Microbes secrets out of organic acid or alkalescence metabolite, causes fermentation liquid pH value to present fluctuation, fermenting and producing mistake
Cheng Zhongxu supplements nertralizer in good time, to maintain suitably fermentation acid-base value environment.
Existing culture tank device, mainly include tank body, agitator, temperature control tubulation, air inlet pipe, escape pipe,
Feed pipe, drainage conduit, temperature electrode, pH electrode and dissolved oxygen electrode.Existing culture tank is typically by single pipe
Liquid or powder nertralizer are added culture tank by road, owing to culture tank is the most bulky, cause nertralizer not
Can spread out rapidly in culture tank, cause local ph too high or too low, be unfavorable for fermentable
Carry out.In addition the interpolation of some solid nertralizer is difficult to, and is easily caused line clogging, is also unfavorable for the carrying out produced.
As a example by lactate fermentation produces, most producers employing calcium hydroxide is as nertralizer, and calcium hydroxide has
Two kinds of addition manners, one is to add with suspension form stream, and another kind is to add with solidapowder form.With
Suspension form adds and there is the easy fouling of calcium hydroxide, the problem of blocking pipeline；And with solidapowder form
Adding delay that membership causes pH value to control and uneven, pH value is wayward.
Summary of the invention
The present invention provides a kind of microorganism culturing tank arrangement, solves the pH value control existing for existing culture tank device
The problem that system is uneven, to optimize nertralizer addition manner, to improve fermentation production efficiency.
A kind of microorganism culturing tank arrangement provided by the present invention, including culture tank, nertralizer storage tank, circulation
Pump and programmable logic controller (PLC) (PLC)；Described culture tank includes tank body and multiple agitator, described tank body
There is temperature regulation structure, for regulating the temperature of tank body；The plurality of agitator in tank interior along tank body
Axis is distributed, and tank body top has air inlet pipe, escape pipe, feed pipe, and tank base has drainage conduit,
The sidewall of tank body inserts pH electrode；Described nertralizer tank bottom is connected with pump inlet by pipeline, follows
Ring pump discharge connects control valve one end and nertralizer feed pipe respectively by pipeline, and nertralizer feed pipe connects
The top of described nertralizer storage tank；Described pH electrode connects programmable logic controller (PLC) (PLC) by wire
Input, the outfan of programmable logic controller (PLC) (PLC) by wire connect control valve control end；
It is characterized in that:
It also includes respiratory organ and multiple nertralizer distributor, and described respiratory organ is microfilter, is loaded on
Tank body top, when charging and discharge, tank body communicates with air；
The described control valve other end connects multiple nertralizer distributor respectively by pipeline, and described nertralizer divides
Cloth device is the Guan Bi circle being made up of stainless steel tube, and circle bottom surface has equally distributed aperture, multiple neutralizations
Agent distributor is distributed along tank body axis in tank interior, and arranges with the plurality of agitator transpostion interval,
The circle place plane of each nertralizer distributor is vertical with tank body axis.
Described microorganism culturing tank arrangement, it is characterised in that:
The temperature regulation structure of described tank body is to be evenly distributed with temperature control tubulation or tank body along the inwall of tank body
Sidewall has the hollow interlayer of cincture, and temperature control tubulation and hollow interlayer are used for being passed through thermal medium or cold medium, right
Tank body heats up or cooling；
Constitute closed circle loop diameter is tank diameter 1/4～the 3/4 of described nertralizer distributor, stainless steel tube
Internal diameter be 0.5cm～30cm.
Described microorganism culturing tank arrangement, it is characterised in that:
Described nertralizer storage tank is 2, and 2 nertralizer storage tanks are in parallel, be respectively used to lay in acid acceptor and
Antalkali.
PH electrode is positioned at the suitable position of tank body and measures fermentation liquid pH value in real time, and provides data to able to programme patrolling
Collect controller (PLC)；Programmable logic controller (PLC) (PLC) is connected with remote monitoring center by data wire
Connect, carry out data process and control the switch of valve；Nertralizer storage tank, tank body uses firm corrosion resistant
Material, such as stainless steel or plastic material, in case nertralizer burn into is revealed；Circulating pump is according to actual work
Skill need select, in order in nertralizer is pressed into culture tank and make nertralizer flowing prevent precipitation.
After fermenting and producing starts, start circulating pump, make nertralizer at pipeline internal circulation flow, along with fermentation
Carrying out, the pH value of fermentation liquid changes, by pH electrode, programmable logic controller (PLC) (PLC) and control valve
The robot control system(RCS) of door composition, the pH value controlling seed or fermentation liquid is setup parameter, according to the pH value set
Parameter, controls valve and is turned on and off, pump into nertralizer in culture tank, to neutralize the organic of constantly generation
Acid or alkaline matter, it is achieved fermentation liquid pH value is automatically adjusted to setup parameter.
Many large-tonnage fermenting and producing products rely primarily on yield and win victory, and process units and production technology are only
Accomplish to become more meticulous and could will reduce production cost, produce preferable economic benefit.The present invention sets in culture tank
Put multilamellar nertralizer distributor, by circulating system, nertralizer can the most uniformly be sent into each portion in tank
Position, improves nertralizer and adds precision, it is to avoid the nertralizers such as calcium hydroxide be converted into calcium carbonate and in the duct
Fouling and clogging pipeline, it is ensured that in tank, pH value fluctuates in controlled range, improves fermenting and producing level, is suitable for
It is the fermenting and producing producing bacterium in using the unicellular microorganism such as antibacterial and yeast.
Accompanying drawing explanation
Fig. 1 is that installation for fermenting of the present invention forms structural representation.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is further described.
As it is shown in figure 1, the present invention includes culture tank 100, nertralizer storage tank 200, circulating pump 300 and can compile
Journey logic controller (PLC) 400；Described culture tank 100 includes tank body 110 and multiple agitator 130, institute
State tank body 110 and there is temperature regulation structure, for regulating the temperature of tank body 110；The plurality of agitator 130
Be distributed along tank body axis tank body 110 is internal, tank body 110 top have air inlet pipe 111, escape pipe 112,
Feed pipe 113, has drainage conduit 114 bottom tank body 110, the sidewall of tank body 110 inserts pH electrode 410；
Being connected with circulating pump 300 import by pipeline bottom described nertralizer storage tank 200, circulating pump 300 outlet is logical
Piping connects control valve 310 one end and nertralizer feed pipe 320 respectively, and nertralizer feed pipe 320 connects
The top of described nertralizer storage tank 200；Described pH electrode 410 connects programmable logic controller (PLC) by wire
(PLC) input of 400, the outfan of programmable logic controller (PLC) (PLC) 400 connects control by wire
The control end of valve 310 processed；
It also includes respiratory organ 140 and multiple nertralizer distributor 150, and described respiratory organ is microfilter,
Being loaded on tank body 110 top, when charging and discharge, tank body 110 communicates with air；
Described control valve 310 other end connects multiple nertralizer distributor 150 respectively by pipeline, described in
Mediating recipe distributor 150 is the Guan Bi circle being made up of stainless steel tube, and circle bottom surface has equally distributed aperture,
Multiple nertralizer distributors 150 are distributed along tank body axis tank body 110 is internal, and with the plurality of stirring
Device 130 transpostion interval arranges, and the circle place plane of each nertralizer distributor is vertical with tank body axis.
Embodiment 1, tank volume 500m3, the inwall of described tank body 110 is evenly distributed with temperature control tubulation 120,
For being passed through thermal medium or cold medium, heating up tank body or cooling, respiratory organ 140 is the micropore mistake of 0.2 μm
Filter；
Nertralizer storage tank 200 is 1, lays in antalkali；Constitute the Guan Bi of described nertralizer distributor
The 1/4 of circle a diameter of tank body 110 diameter, the internal diameter of stainless steel tube is 30cm.
Use this device to use lactic acid bacteria to carry out lactate fermentation production, use high temperature resistant anaerobism lactic acid bacteria (dry
Lactobacillus casei Lactobacillus casei B9232) carry out fermentation culture.By culture tank steam sterilization
Add sterilized culture medium after sterilization, use temperature control tubulation to control culture medium temperature at 40 DEG C；To plant again
Son joins in culture tank 100, and the calcium hydroxide adding sterilization in nertralizer storage tank 200 is suspended
Liquid, opens circulating pump 300, makes calcium hydroxide suspension at nertralizer feed pipe 320 internal circulation flow in order to avoid sinking
Fall fouling.Programmable logic controller (PLC) (PLC) 400 is used to detect data according to pH electrode 410, by control
Valve 310 processed controls the interpolation of nertralizer calcium hydroxide, finally makes the pH stable during fermentation culture exist
6.5.About about 37 hours, fermentation ends put tank, put tank lactic acid concn 178g/L, fermentation time reduction,
Lactic acid production improves.
Embodiment 2, tank volume 8L, the sidewall of described tank body 110 has the hollow interlayer of cincture, is used for
Being passed through thermal medium or cold medium, heat up tank body or cooling, respiratory organ 140 is the microfilter of 0.2 μm；
Nertralizer storage tank 200 is 2, and 2 nertralizer storage tanks 200 are in parallel, is respectively used to lay in acid neutralization
Agent and antalkali；Constitute closed circle loop diameter is tank body 110 diameter the 3/4 of described nertralizer distributor,
The internal diameter of stainless steel tube is 0.5cm.
Use this device to use yeast to carry out fermenting and producing active yeast mycelium, use sugar-free yeast strain
(bakery yeast Saccharomyces cerevisiae CICC 1525) carries out fermentation culture.By culture tank
With adding sterilized culture medium after steam sterilizing disinfecting, hollow interlayer is used culture medium temperature to be controlled
32℃；Again seed is joined in culture tank 100, use stream to add the mode feed-batch culture of molasses.At two sets
In nertralizer storage tank 200 in parallel, it is separately added into the sulphuric acid of sterilized 30% and the sodium carbonate liquor of 30%.
At earlier fermentation, fermentation liquid pH value has downward trend, opens circulating pump 300, first make 30% sodium carbonate molten
Liquid, at nertralizer feed pipe 320 internal circulation flow, uses programmable logic controller (PLC) (PLC) 400 according to pH
Electrode 410 detects data, controls the interpolation of nertralizer sodium carbonate liquor by controlling valve 310, makes fermentation
PH stable in incubation is 4.3；In the fermentation later stage, fermentation liquid pH value is on the rise, by nertralizer
In feed pipe 320, sodium carbonate liquor is discharged, and is joined in nertralizer feed pipe 320 by the sulphuric acid of 30%, makes to send out
PH stable in ferment incubation is 4.3.About about 20 hours, fermentation liquid weight in wet base reached 200g/L,
The fermentative activity hit the target 650 of yeast terminates fermentation and puts tank, fermentation time reduction, and yeast yield carries
High.
Claims (3)
1. a microorganism culturing tank arrangement, including culture tank (100), nertralizer storage tank (200), follows
Ring pump (300) and programmable logic controller (PLC) (400)；Described culture tank (100) includes tank body (110)
With multiple agitators (130), described tank body (110) has temperature regulation structure, is used for regulating tank body (110)
Temperature；The plurality of agitator (130) is internal along the distribution of tank body axis, tank at tank body (110)
Body (110) top has air inlet pipe (111), escape pipe (112), feed pipe (130), tank body (110)
Bottom has drainage conduit (114), and the sidewall of tank body (110) inserts pH electrode (410)；Described nertralizer stores up
Tank (200) bottom is connected with circulating pump (300) import by pipeline, and circulating pump (300) outlet is by pipe
Road connects control valve (310) one end and nertralizer feed pipe (320) respectively, and nertralizer feed pipe (320) is even
The top of logical described nertralizer storage tank (200)；Described pH electrode (410) connects FPGA by wire
The input of controller (400), the outfan of programmable logic controller (PLC) (400) is connected by wire
Control the control end of valve (310)；It is characterized in that:
It also includes respiratory organ (140) and multiple nertralizer distributor (150), and described respiratory organ is micropore
Filter, is loaded on tank body (110) top, and when charging and discharge, tank body (110) communicates with air；
Described control valve (310) other end connects multiple nertralizer distributor (150) respectively by pipeline,
Described nertralizer distributor (150) is the Guan Bi circle being made up of stainless steel tube, and circle bottom surface has uniformly to be divided
The aperture of cloth, multiple nertralizer distributors (150) are internal along the distribution of tank body axis at tank body (110), and
Arrange with the plurality of agitator (130) transpostion interval, the circle place plane of each nertralizer distributor and tank
Body axis is vertical.
2. microorganism culturing tank arrangement as claimed in claim 1, it is characterised in that:
The temperature regulation structure of described tank body (110) is to be evenly distributed with temperature control tubulation along the inwall of tank body
(120) or the sidewall of tank body has the hollow interlayer of cincture, temperature control tubulation and hollow interlayer are used for being passed through
Thermal medium or cold medium, heat up to tank body or cooling；
Constitute closed circle loop diameter is tank body (110) diameter the 1/4～3/4 of described nertralizer distributor, no
The internal diameter of rust steel pipe is 0.5cm～30cm.
3. microorganism culturing tank arrangement as claimed in claim 1 or 2, it is characterised in that:
Described nertralizer storage tank (200) is 2, and 2 nertralizer storage tanks (200) are in parallel, are respectively used to deposit
Acid acceptor and antalkali.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610321215.2A CN105838594B (en) | 2016-05-16 | 2016-05-16 | A kind of microculture tank arrangement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610321215.2A CN105838594B (en) | 2016-05-16 | 2016-05-16 | A kind of microculture tank arrangement |
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| CN105838594A true CN105838594A (en) | 2016-08-10 |
| CN105838594B CN105838594B (en) | 2017-06-13 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101153296A (en) * | 2007-09-19 | 2008-04-02 | 北京化工大学 | Method for producing L-lactic acid by feedback control of substrate concentration with neutralizer |
| CN201390742Y (en) * | 2009-04-16 | 2010-01-27 | 河南科技大学 | Microorganism culture tank and microorganism culture apparatus using microorganism culture tank |
| CN103789194A (en) * | 2013-10-12 | 2014-05-14 | 云南龙谷生物科技有限公司 | Microorganism culture tank |
| CN204097451U (en) * | 2014-07-25 | 2015-01-14 | 上海瑞派机械有限公司 | Multifunctional fermentation tank |
| CN104403925A (en) * | 2014-12-21 | 2015-03-11 | 竹溪县宝田生物制品有限公司 | Microbe culture tank |
| CN205616892U (en) * | 2016-05-16 | 2016-10-05 | 湖北广济药业股份有限公司 | Microbial cultivation canning is put |
-
2016
- 2016-05-16 CN CN201610321215.2A patent/CN105838594B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101153296A (en) * | 2007-09-19 | 2008-04-02 | 北京化工大学 | Method for producing L-lactic acid by feedback control of substrate concentration with neutralizer |
| CN201390742Y (en) * | 2009-04-16 | 2010-01-27 | 河南科技大学 | Microorganism culture tank and microorganism culture apparatus using microorganism culture tank |
| CN103789194A (en) * | 2013-10-12 | 2014-05-14 | 云南龙谷生物科技有限公司 | Microorganism culture tank |
| CN204097451U (en) * | 2014-07-25 | 2015-01-14 | 上海瑞派机械有限公司 | Multifunctional fermentation tank |
| CN104403925A (en) * | 2014-12-21 | 2015-03-11 | 竹溪县宝田生物制品有限公司 | Microbe culture tank |
| CN205616892U (en) * | 2016-05-16 | 2016-10-05 | 湖北广济药业股份有限公司 | Microbial cultivation canning is put |
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