CN105820946A - Combined floating bacterium sampling head with culture dish - Google Patents
Combined floating bacterium sampling head with culture dish Download PDFInfo
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- CN105820946A CN105820946A CN201610343258.0A CN201610343258A CN105820946A CN 105820946 A CN105820946 A CN 105820946A CN 201610343258 A CN201610343258 A CN 201610343258A CN 105820946 A CN105820946 A CN 105820946A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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Abstract
The invention discloses a combined floating bacterium sampling head with a culture dish .The combined floating bacterium sampling head comprises a sampling head main body (1) which is shaped like a cylinder with the hollow interior, the bottom or side face of the sampling head main body (1) is provided with an air extracting channel (8), the top of the sampling head main body (1) is provided with a sampling cover (4) with air flow channels (5), the culture dish (2) is arranged on the portion, located under the air flow channels (5), of an inner cavity of the sampling head main body (1), the culture dish (2) and the sampling head main body (1) are fixedly connected or are of an integrated structure, and the sampling head main body (1), the sampling cover (4) and the culture dish (2) are made of transparent resin .The novel sampling head used by being matched with a floating bacterium sampler has the advantages of being low in cost, easy to use, capable of decreasing the possibility that a sample is contaminated and the like.
Description
Technical field
The present invention relates to enviromental monitoring equipment field, particularly relate to a kind of combination type flcating germ sampling head containing culture dish.
Background technology
Flcating germ detection is a kind of method that medical industry is manufactured and is sampled microorganism floating in environment, cultivates, counts during microorganism detection etc., the most again with the production of aseptic medicine, inspection etc. for very, it is the monitoring means of a kind of routine, is distinctly claimed in relevant laws and regulations and relevant criterion simultaneously.As " Good Manufacturing Practice and Quality Control of Drug 2010 editions " annex I sterile preparation Article 11 is done specified below: " microorganism should be carried out dynamic monitoring, the microbial status of assessment sterile production ".Owing to this monitoring project is regulation compulsive requirement, therefore each aseptic medicine manufacturing enterprise (including aseptic medicine inspection body) is both needed to carry out this detection.
Detection method typically have settling bacteria method, certain amount of air flcating germ sampling method and surface area method (as cotton swab method with contact dish method) etc..Due to the principle of flcating germ sampling be by vacuum pump, the air in space is extracted after sampling head above oversampler, impinge upon in the culture medium being positioned at below sampling head to the uniform constant speed of air, thus the flcating germ comprised in air is i.e. attached to media surface, after certain time, culture medium is cultivated, thus pass judgment on the level of flcating germ in air.Air owing to sucking all have to be through being positioned at the sampling head above culture medium, so the aseptic of sampling head itself is particularly important in sampling process, pollutes if itself exists, can cause the false positive of assay.
Sampling head is sold usually used as consumptive material, therefore: (1) enterprise is that a sampler is equipped with multiple sampling heads, uses after unified sterilizing, and each sampled point is used alone the sampling head after a sterilizing；Shortcoming: sampling head is usually stainless steel products, expensive, each about in 3000～10000 RMB, so can strengthen entreprise cost；And each sampling head is required to the process of sterilizing-pack-unpack-use, sampling cost is big every time and waste man-hour.Or (2) enterprise is that a sampler is only equipped with a sampling head, reuse after sterilizing with the disinfectant such as ethanol or other disinfection way after a single use；, there is the risk of cross-contamination between each sampled point, if there is positive, it is impossible to judge the source of contaminated samples accurately in shortcoming: the disinfectant such as ethanol cannot guarantee to reach the level of Sterility Assurance；And the sampling head after sterilization every time exists the possibility of disinfectant residual, thus produce bacteriostasis, cause false negative risk；According to the means such as high temperature or irradiation sterilization, then between sampling, duration is long every time, greatly reduces work efficiency.
And operating process during flcating germ monitoring under traditional structure is: open sampling head-put into culture dish-open culture dish lid-cover sampling head-carry out and sample-open sampling head-cover culture dish lid-taking-ups culture dish-preparation cultivation.Above-mentioned flow operations is complicated, and in sampling operation, multiple steps easily exist the contaminated risk of culture medium, ultimately result in testing result incorrect.
Summary of the invention
Because the drawbacks described above of prior art, the technical problem to be solved be to provide a kind of with borne bacteria sampler with the use of novel sampling head, have with low cost, it is easy to use, reduce the advantages such as the contaminated probability of sample.
For achieving the above object, the invention provides a kind of combination type flcating germ sampling head containing culture dish, including sampling head main body, described sampling head main body is the cylindric of inner hollow, sampling head bottom part body or side are provided with bleed-off passage, sampling head body top is provided with the sampling lid of gas channel, at described sampling head body lumen and be positioned at below gas channel and be provided with culture dish, described culture dish and described sampling head main body be fixing to be connected or is integral type structure, and described sampling head main body, described sampling lid and described culture dish are made up of transparent resin.
Preferably, described sampling lid is groove structure, and described gas channel is positioned at the bottom surface of groove.The sampling lid of groove structure is as the inlet channel of air, compared to the sampling lid of burnishing surface, more can form stable inlet air flow, the culture medium in culture dish below air energy stable and uniform shock.
Preferably, described sampling lid for detachable or with described sampling head main body integral structure.Sampling lid for detachable design, when can conveniently produce when culture dish is integral type with sampling head main body to culture dish in culture medium fill, it is also possible to meet actually used middle needs and culture medium carried out the demand of other operation.
Preferably, described gas channel is the multiple slits radially extended along groove floor or the multiple through holes being distributed in groove floor.Gas channel is preferably the multiple slits radially extended along groove floor, and when gas channel is slit, sampling resistance is little, and can carry out isokinetic sampling, and sampling flow is big, and the flcating germ sampled is evenly distributed.
Preferably, described culture dish is fixed connected mode with described sampling head main body and is: described sampling head body lumen wall is provided with several connectors, and described culture dish is provided with several spacing holes matched with connector.Sampling head main body and culture dish can multiple connected mode, connector and spacing hole be connected as wherein one of simple connected mode, there is connected mode simple, the advantages such as manufacture difficulty is low, easy to operate.The gas channel size between culture dish and lower section sampler can also be regulated by regulation hole depth (i.e. connection gap size between regulation culture dish and sampling head main body).
Preferably, described culture dish is fixed connected mode with described sampling head main body and is: be provided with, with described culture dish edge, the multiple thread cooperated on the wall of described sampling head body lumen limit.When so both screw threads screw, culture dish completely cuts off with lower air, it is achieved the sealing of lower section, when somewhat unscrewing, connects with lower air, can sample, then when unscrewing, culture dish just can take off.So connect and can need not seal the seal members such as lower cover, reduce production cost, and reduce further the contaminated risk of culture medium.
Preferably, also including sealing upper cover and sealing lower cover, described sealing upper cover is used for hiding described gas channel, and described sealing lower cover is for hiding the bleed-off passage of described sampling head main body.Sampling head can be fully sealed by sealing upper cover and sealing lower cover, can not be contaminated with the culture medium in the sampling head after protecting new sampling head and sampling.
Preferably, described sealing upper cover is connected with described sampling lid rotating shaft.Sampling process need not take down sealing upper cover, seal upper cover and be connected as a single entity with sampling head, it is to avoid seal upper cover and can put nowhere, cause the probability polluted when losing or place.
The present invention has following technical effect that the present invention is relative to prior art, knockdown flcating germ sampling head is used to be used in combination with borne bacteria sampler, sampling head comprises culture dish, without culture dish is operated, eliminate culture dish and the step such as uncap, place and add a cover, decrease operating procedure, improve work efficiency, and possibility of pollution can be reduced.
The easy mold of transparent resin, and rustless steel relatively is more cheap, reduce the purchase cost of enterprise, the front sampling head Aseptic sterilisation of use of dispatching from the factory, sample detecting is complete the most discardable, without the program of carrying out disinfection before and after sampling, therefore sampling head can reduce enterprise's use cost with single use and increase work efficiency.And transparent combination type sampling head, after being greatly simplified operating procedure, and a sampling process, culture dish is without taking out, and whole sampling head is delivered to cultivate and detection, decreases the culture medium risk by external contamination, improves the accuracy of testing result.
Accompanying drawing explanation
Fig. 1 is the generalized section of a preferred embodiment sampling head of the present invention；
Fig. 2 is the generalized section of a preferred embodiment sampling head of the present invention；
Fig. 3 is the generalized section of a preferred embodiment sampling head of the present invention；
Fig. 4 is the top view of a preferred embodiment sampling lid of the present invention；
Fig. 5 is the top view of another preferred embodiment sampling lid of the present invention；
Fig. 6 is the combination schematic diagram of a preferred embodiment sampling head of the present invention；
In figure, 1 is sampling head main body, and 2 is culture dish, and 3 is culture medium, and 4 is sampling lid, and 5 is gas channel, and 6 is connector, and 7 is spacing hole, and 8 is bleed-off passage, and 9 for sealing upper cover, and 10 for sealing lower cover.
Detailed description of the invention
Below with reference to accompanying drawing, the technique effect of design, concrete structure and the generation of the present invention is described further, to be fully understood from the purpose of the present invention, feature and effect.
As shown in Figure 1 with borne bacteria sampler with the use of sampling head, including sampling head main body 1, sampling head main body 1 is the cylindrical-shaped structure of inner hollow, sampling head main body 1 top is provided with the sampling lid 4 of gas channel 5, and outside air covers the gas channel 5 of 4 then into sampling head inside by sampling under the suction of the actuators such as sampler blower fan.Sampling head main body 1 intracavity bottom can be closed, now sampling head main body 1 side arranges bleed-off passage 8 (as shown in Figure 2), the exhaust tube of sampler by realizing the connection with sampling head and bleeding with bleed-off passage 8, or the bottom of sampling head main body 1 is completely open as bleed-off passage 8, then it is provided with screw thread, buckle or other type of attachment in mating connection with existing sampler at intracavity bottom limit wall, (according to the sampler of different size model, arrange corresponding sampling head type of attachment) is bled again after being connected of various samplers with on market.Be provided with the culture dish 2 being loaded with culture medium 3 at sampling head main body 1 inner chamber being positioned at below gas channel 5, culture dish 2 area is generally higher than the gross area shared by gas channel 5.
Can fix between culture dish 2 with sampling head main body 1 and be connected, connected mode can be arbitrary connected mode of the prior art, as snapped connection, inlay or being gluing etc..As a kind of embodiment, sampling head main body 1 internal chamber wall is provided with several connectors 6 (such as symmetrical two, or three of one-tenth angular distribution, typically it is not provided with too many, it is to avoid affect air circulation), connector 6 can be inner chamber roof arrange straight down also can inner chamber limit wall horizontally arranged, accordingly, culture dish 2 is provided with several spacing holes 7 matched with connector 6, when being in connection status, connector 6 inserts spacing hole 7 and achieves a fixed connection, as shown in Figure 1.Or, the connector 6 of sampling head main body 1 is designed as band barbed configuration and has certain elasticity, as it is shown on figure 3, and culture dish 2 edge is provided with circumferential projection platform, during connection status, the barb of connector 6 is buckled in recess below projection, it is achieved fixing connect.As another embodiment, sampling head main body 1 is threadeded each other with culture dish 2 phase, such as, arrange multiple thread on the wall of sampling head main body 1 inner chamber limit, accordingly, culture dish 2 edge is provided with the multiple thread matched, so, when screwing, sampling head main body 1 limit wall fits tightly with culture dish 2 edge, between the two tight and realize seal, when somewhat unscrewing, reserve space between the two, the most just can be sampled operation, when unscrewing further, just can take off culture dish 2.Fixing connected mode between sampling head main body 1 and culture dish 2, culture dish 2 can be dismounted, with meet some actually used time specific demand, simultaneously, can also provide convenient in sampling head production and processing, as first to culture dish 2 fill culture medium, the most again culture dish 2 assembled with sampling head main body 1.Certainly being alternatively one-body molded formula structure between culture dish 2 and sampling head main body 1, compared to removable fixing connected mode, integral type can simplify operation to a certain extent, it is also possible to reduces production cost, reduces production difficulty.
Being filled with culture medium 3, the culture medium 3 in the aforementioned air impact entered within sampling head to culture dish 2 in culture dish 2, the flcating germ in air is attached in culture medium 3, flows out from the space between sampling head main body 1 and culture dish 2 after right air.Sampling head main body 1, sampling lid 4 and culture dish 2 are made up of transparent resin.Resin is easier to mold molding, and cheap, can realize single use, and cost is relatively low.And whole sampling head (including sampling head main body 1 and culture dish 2) is transparent, therefore after a sampling process, it is not necessary to take out culture dish 2, whole sampling head may be regarded as common culture dish 2, then cultivates and detects.
Do not consider culture dish 2, for convenience of illustrating, sampling head is divided into sampling lid 4 and about 1 two part of sampling head main body by us, sampling lid 4 be between outside air and culture dish 2 through passage, sampling lid 4 can be the structure of plane, and gas channel 5 is distributed on the surface of sampling lid 4, it is also possible to for the groove structure of concave downward, groove has certain degree of depth, and gas channel 5 is distributed in groove floor.Sampling lid 4 can have a style of one's own with sampling head main body 1, it is also possible to removably links together with sampling head main body 1.Detachable design can facilitate the fill of culture medium when producing sampling head main body 1 with culture dish 2 integral type, second can meet needs in some real processes and the most afterwards culture medium be carried out other operational requirements first.Gas channel 5 on sampling lid 4 can be multiple slits that sampling lid 4 centers, edge (as being then the bottom center of groove) radially extend for the sampling lid 4 of groove, as shown in Figure 4, or it is distributed in sampling lid 4 surfaces (if the sampling lid 4 for groove is then for the bottom surface of groove) multiple through holes, as shown in Figure 5.
As shown in Figure 6, sampling head also includes sealing upper cover 9 and sealing lower cover 10, seal upper cover 9 for hiding the gas channel 5 of sampling lid 4, realize from top isolation outside air and culture dish 2, seal lower cover 10 to be used for hiding sampling head main body 1 bottom opening, realize from below isolation outside air and culture dish 2 (if illustrating as aforementioned, it it is thread connecting mode between sampling head main body 1 and culture dish 2, seal lower cover 10 can need not, can also be plus sealing lower cover 10, avoid after this connected mode down-sampling, forgetting to screw culture dish 2 and causing unsealing below culture dish 2).Seal form is varied, as a example by sealing upper cover 9, sealing upper cover 9 can be directly more than sampling lid 4, upper cover directly covers sampling lid 4 realization and seals, can also be as the most consistent with the groove footpath of groove during groove type sampling lid 4, upper cover is directly embedded into groove and realizes sealing, and now upper lid surface is generally with being available for the structure (such as draw ring or stress platform) of stress operation so that the upper cover embedded can be extracted realization and open.Certainly, can also be connected for rotating shaft between upper cover 9 with sampling lid 4 as sealed, can directly open during sampling and seal upper cover (or the mode of directly horizontal rotation open sealing upper cover), after sampling, closing seam upper cover realizes sealing, and and for example arranges slidably and flexible slide plate between sampling lid 4 gas channels 5 and culture dish, and sampling has covered exercisable operating parts, during Guan Bi, slide plate trails covering gas channel, and when opening, slide plate contracting is folded exposes covered gas channel.The form sealing upper cover 9 and sealing lower cover 10 can be varied, does not repeats them here.Seal upper cover and sealing lower cover is the most all made up of transparent resin material, single use can be realized.
As a example by the embodiment shown in Fig. 6, the occupation mode of the sampling head that the present invention provides is specific as follows:
Open sealing lower cover 10, sampling head is arranged on matched sampler, then open sealing upper cover 9, sample, then cover sealing upper cover 9, take off sampling head, cover sealing lower cover 10, whole sampling head is sent to cultivation and the detection of flcating germ.
The preferred embodiment of the present invention described in detail above.Should be appreciated that those of ordinary skill in the art just can make many modifications and variations according to the design of the present invention without creative work.Therefore, all technical staff in the art, all should be in the protection domain being defined in the patent claims the most on the basis of existing technology by the available technical scheme of logical analysis, reasoning, or a limited experiment.
Claims (8)
1. the combination type flcating germ sampling head containing culture dish, it is characterized in that, including sampling head main body (1), described sampling head main body (1) is the cylindric of inner hollow, sampling head main body (1) bottom or side are provided with bleed-off passage (8), sampling head main body (1) top is provided with the sampling lid (4) of gas channel (5), described sampling head main body (1) inner chamber and be positioned at gas channel (5) lower section be provided with culture dish (2), described culture dish (2) and described sampling head main body (1) be fixing to be connected or is integral type structure, described sampling head main body (1), described sampling lid (4) and described culture dish (2) are made up of transparent resin.
2. the combination type flcating germ sampling head containing culture dish as claimed in claim 1, it is characterised in that described sampling lid (4) is groove structure, and described gas channel (5) is positioned at the bottom surface of groove.
3. the combination type flcating germ sampling head containing culture dish as claimed in claim 2, it is characterised in that described sampling lid (4) be detachably or with described sampling head main body (1) integral structure.
4. the combination type flcating germ sampling head containing culture dish as claimed in claim 2, it is characterised in that described gas channel (5) is the multiple slits radially extended along groove floor or the multiple through holes being distributed in groove floor.
5. the combination type flcating germ sampling head containing culture dish as claimed in claim 1, it is characterized in that, described culture dish (2) connected mode fixing with described sampling head main body (1) is: described sampling head main body (1) internal chamber wall is provided with several connectors (6), and described culture dish (2) is provided with several spacing holes (7) matched with connector (6).
6. the combination type flcating germ sampling head containing culture dish as claimed in claim 1, it is characterized in that, described culture dish (2) connected mode fixing with described sampling head main body (1) is: be provided with, with described culture dish (2) edge, the multiple thread cooperated on the wall of described sampling head main body (1) inner chamber limit.
7. the combination type flcating germ sampling head containing culture dish as claimed in claim 1, it is characterized in that, also include sealing upper cover (9) and sealing lower cover (10), described sealing upper cover (9) is used for hiding described gas channel (5), and described sealing lower cover (10) is used for hiding the bleed-off passage (8) of described sampling head main body (1).
8. the combination type flcating germ sampling head containing culture dish as claimed in claim 7, it is characterised in that described sealing upper cover is connected with described sampling lid rotating shaft.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610343258.0A CN105820946A (en) | 2016-05-23 | 2016-05-23 | Combined floating bacterium sampling head with culture dish |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610343258.0A CN105820946A (en) | 2016-05-23 | 2016-05-23 | Combined floating bacterium sampling head with culture dish |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3968012A (en) * | 1975-06-06 | 1976-07-06 | Jones Jay A | Aerosol bacterial contamination test kit |
| US20070269849A1 (en) * | 2004-10-06 | 2007-11-22 | Pierre Bridenne | Air Sampling Method, Device and System for Microbiological Analysis |
| US20150075301A1 (en) * | 2013-07-23 | 2015-03-19 | Particle Measuring Systems, Inc. | Microbial air sampler integrating media plate and sample collection device |
| CN205774522U (en) * | 2016-05-23 | 2016-12-07 | 郑振华 | A kind of combination type flcating germ sampling head containing culture dish |
-
2016
- 2016-05-23 CN CN201610343258.0A patent/CN105820946A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3968012A (en) * | 1975-06-06 | 1976-07-06 | Jones Jay A | Aerosol bacterial contamination test kit |
| US20070269849A1 (en) * | 2004-10-06 | 2007-11-22 | Pierre Bridenne | Air Sampling Method, Device and System for Microbiological Analysis |
| US20150075301A1 (en) * | 2013-07-23 | 2015-03-19 | Particle Measuring Systems, Inc. | Microbial air sampler integrating media plate and sample collection device |
| CN205774522U (en) * | 2016-05-23 | 2016-12-07 | 郑振华 | A kind of combination type flcating germ sampling head containing culture dish |
Non-Patent Citations (1)
| Title |
|---|
| 齐宝森等: "《新型材料及其应用》", 30 September 2007 * |
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Application publication date: 20160803 |