CN105820942B - A kind of particle gun piston specimen holder - Google Patents
A kind of particle gun piston specimen holder Download PDFInfo
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- CN105820942B CN105820942B CN201610349894.4A CN201610349894A CN105820942B CN 105820942 B CN105820942 B CN 105820942B CN 201610349894 A CN201610349894 A CN 201610349894A CN 105820942 B CN105820942 B CN 105820942B
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- cylinder
- piston
- cup
- particle gun
- specimen holder
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
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- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Investigating Strength Of Materials By Application Of Mechanical Stress (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of particle gun piston specimen holder, it is characterised in that：It includes a both ends open and the cylinder of vertical arrangement, is slided on the inwall of the cylinder and sets a piston, the cup that the piston is used to hold acceptor material by multiple spaced apart support bar connections one.The bottom of the cup is arranged to slide glass with holes, is arranged to frosting on the inside of the slide glass.On the cylinder multiple through holes are offered in the side wall above the cup；Slideway is provided with the inwall of the cylinder, the piston is slidably connected with the slideway；Gap is provided between the cylinder and the cup；Support frame is provided with the outer wall of the cylinder bottom；The bottom of the slideway is provided with external part；Scale value is provided with the outer wall of the cylinder.The present invention can reduce the gas shock to acceptor material, prevent that acceptor material from being dispelled, simple in construction so as to improve the hit efficiency of particle gun, easy to use.
Description
Technical field
The present invention relates to a kind of specimen holder, especially with regard to a kind of particle gun piston specimen holder.
Background technology
Particle bombardment is a kind of brand-new gene Transfection Technology, the micro- bullet of high speed that will be attached with inhereditary material or other materials
Cell, tissue and organelle are emitted directly toward, is current gene Transfection Technology state-of-the-art in the world, there is quick, easy, safety
And the characteristics of efficient.The gas shock wave that gas gene gun is changed into using compressed gas (helium or nitrogen) is power, by gas gene
The gas shock wave of a kind of " cold " caused by rifle is sent into bombardment room, so as to avoid cellular damage caused by " heat " gas shock wave.
Gas gene gun can obtain instantaneous, stable and efficient transformation in extensive cellular type.Gas gene gun
There is the special construction of a generation shock wave, have in appropriate air pressure range from 3.5~10MPa corresponding different rupturable
Film, the particle for coating DNA is passed through, finally injected in the target cell of bombardment room bottom.Particle gun is applied to animals and plants, cell
Culture, embryo, the transgenosis of bacterium and meiofauna.
When in the prior art, using powdered acceptor materials such as Bombardment-Mediated Transformation pollen, most of experimenter be by
Pollen is laid on glass dish or is positioned over closed sample room, is placed in bombarding under particle gun.Such as Dong Yunzhou with millet into
During ripe pollen is the genetic transformation of carrier, Ji Gu 11 mature pollen is taken, after sieving, going the removal of impurity, by clean flower
Powder is laid on glass dish, is finally placed under particle gun and is bombarded, and bombardment finishes, and picks pollen with writing brush at once.In addition, Liu Danmei etc.
During using biolistic bombardment mature flower powder maize transformation, sample is put into sample room first, then adds and stops net.It is but sharp
During with the dusty material such as glass dish or closed sample room conversion pollen, after particle gun is shot, very big air-flow can be produced.Powder
Last shape acceptor material after powerful gas shock, is easily dispelled, therefore have impact on gene due to light weight, small volume
The hit efficiency of rifle.
The content of the invention
A kind of in view of the above-mentioned problems, particle gun piston high and easy to use it is an object of the invention to provide hit efficiency
Specimen holder.
To achieve the above object, the present invention takes following technical scheme：A kind of particle gun piston specimen holder, its feature exist
In：It includes a both ends open and the cylinder of vertical arrangement, is slided on the inwall of the cylinder and sets a piston, the piston
The cup for being used to hold acceptor material by multiple spaced apart support bar connections one.
The bottom of the cup is arranged to slide glass with holes, is arranged to frosting on the inside of the slide glass.
On the cylinder multiple through holes are offered in the side wall above the cup.
Slideway is provided with the inwall of the cylinder, the piston is slidably connected with the slideway.
Gap is provided between the cylinder and the cup.
Support frame is provided with the outer wall of the cylinder bottom.
The bottom of the slideway is provided with external part.
Scale value is provided with the outer wall of the cylinder.
For the present invention due to taking above technical scheme, it has advantages below：1st, the present invention is provided with a cylinder, in cylinder
Inwall on slide set a piston, in order to which piston moves down along cylinder inboard wall.2nd, inventive piston passes through multiple supports
Bar connects a cup, when piston is moved down by gas shock, drives cup to move down, reduces the air-flow of acceptor material
Impact, rate is hit so as to increase particle gun.3rd, the present invention is arranged to slide glass with holes in the bottom of cup, beneficial to cylinder
Interior gas discharge, it can prevent that particle gun from shooting the gas rapid expanding in rear cylinder body.4th, the present invention is located at cup on cylinder
Multiple through holes are offered in side wall above body, is discharged beneficial to the gas in cylinder, can prevent particle gun from shooting in rear cylinder body
Gas rapid expanding.5th, it is arranged to frosting on the inside of slide glass of the present invention, by increasing capacitance it is possible to increase the frictional force between slide glass and cup,
Powdered acceptor material is set to keep geo-stationary in cup.6th, the present invention is provided with gap between cylinder and cup, is beneficial to
Gas discharge in cylinder, can also prevent that particle gun from shooting the gas rapid expanding in rear cylinder body.7th, the present invention is in cylinder
Scale value is provided with outer wall, for observing cup in the position of inner barrel, in order to adjust between particle gun and cup
Distance.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention
Fig. 2 is that the present invention removes the schematic top plan view after support frame
Embodiment
The present invention is described in detail with reference to the accompanying drawings and examples.
As shown in Figure 1 and Figure 2, the present invention proposes a kind of particle gun piston specimen holder, and it includes a both ends open and hung down
To the cylinder 1 of arrangement, slided on the inwall of cylinder 1 and a piston 2 is set, piston 2 is connected by multiple spaced apart support bars 3
Connect a cup 4 for being used to hold acceptor material.
In above-described embodiment, as shown in figure 1, being arranged to holes 5 slide glass 6 in the bottom of cup 4, the aperture in hole 5 is less than
Acceptor material, discharged beneficial to the gas in cup 4, avoid particle gun 7 from shooting the air rapid expanding in rear cylinder body 1.In slide glass 6
Inner side be arranged to frosting, by increasing capacitance it is possible to increase the frictional force between acceptor material and slide glass 6, so, when cup 4 moves, by
Body material can keep geo-stationary in cup 4.
In above-described embodiment, as shown in figure 1, multiple through holes 8 are offered in the side wall above cup 4 on cylinder 1,
Discharged beneficial to the gas in cylinder 1.
In above-described embodiment, as shown in figure 1, being provided with slideway 9 on the inwall of cylinder 1, piston 2 slides with slideway 9 to be connected
Connect.
In above-described embodiment, as shown in figure 1, gap is provided between cylinder 1 and cup 4, beneficial to the gas in cylinder 1
Discharge.
In above-described embodiment, as shown in figure 1, support frame 10 is provided with the outer wall of the bottom of cylinder 1, in order to realize cylinder
Body 1 is hanging.
In above-described embodiment, as shown in figure 1, external part 11 can be provided with the bottom of slideway 9, when particle gun 7 is shot
Afterwards, after cup 4 moves down in the presence of air-flow, for supporting support bar 3.
In above-described embodiment, scale value is provided with the outer wall of cylinder 1, for observing position of the cup 4 inside cylinder 1
Put, in order to adjust the distance between particle gun 7 and cup 4.
In above-described embodiment, as shown in figure 1, a diameter of 7~9 centimetres of cylinder 1, a height of 16~20 centimetres.
In above-described embodiment, as shown in figure 1, a diameter of 3.5~4.5 centimetres of cup 4, a height of 3.5~4.5 centimetres.Its
In, bombardment scope radius of the particle gun 7 within 14 centimetres of ranges is not more than 1.7 centimetres, because particle gun is in 14 centimetres of ranges
Within bombardment scope radius be not more than 1.7 centimetres, so the diameter design of cup 4 be 3.5~4.5 centimetres.
In above-described embodiment, as shown in Figure 1 and Figure 2, piston 2 is made of elastomeric material.
As shown in figure 1, acceptor material in use, be placed in cup 4 by the present invention first.When carrying out genetic transformation,
After being shot using particle gun 7, very big air-flow is produced in cup 4.In the presence of air-flow, piston 2 can be promoted along slideway 9
Move down, to reduce the gas shock to acceptor material, prevent that acceptor material from being dispelled, so as to increase hitting for particle gun 7
Efficiency.
The various embodiments described above are merely to illustrate the present invention, wherein the structure of each part, connected mode etc. are all can be
Change, every equivalents carried out on the basis of technical solution of the present invention and improvement, it should not exclude the present invention's
Outside protection domain.
Claims (8)
- A kind of 1. particle gun piston specimen holder, it is characterised in that：It includes a both ends open and the cylinder of vertical arrangement, in institute State to slide on the inwall of cylinder and a piston be set, the piston by multiple spaced apart support bars connections one be used to holding by The cup of body material.
- A kind of 2. particle gun piston specimen holder as claimed in claim 1, it is characterised in that：The bottom of the cup is arranged to Slide glass with holes, it is arranged to frosting on the inside of the slide glass.
- A kind of 3. particle gun piston specimen holder as claimed in claim 1, it is characterised in that：Positioned at described on the cylinder Multiple through holes are offered in side wall above cup.
- A kind of 4. particle gun piston specimen holder as claimed in claim 1, it is characterised in that：Set on the inwall of the cylinder Slideway is equipped with, the piston is slidably connected with the slideway.
- A kind of 5. particle gun piston specimen holder as claimed in claim 1, it is characterised in that：In the cylinder and the cup Between be provided with gap.
- A kind of 6. particle gun piston specimen holder as claimed in claim 1, it is characterised in that：Outer wall in the cylinder bottom On be provided with support frame.
- A kind of 7. particle gun piston specimen holder as claimed in claim 4, it is characterised in that：Set in the bottom of the slideway There is external part.
- A kind of 8. particle gun piston specimen holder as claimed in claim 1, it is characterised in that：Set on the outer wall of the cylinder It is equipped with scale value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610349894.4A CN105820942B (en) | 2016-05-24 | 2016-05-24 | A kind of particle gun piston specimen holder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610349894.4A CN105820942B (en) | 2016-05-24 | 2016-05-24 | A kind of particle gun piston specimen holder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105820942A CN105820942A (en) | 2016-08-03 |
| CN105820942B true CN105820942B (en) | 2017-12-19 |
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ID=56530542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610349894.4A Active CN105820942B (en) | 2016-05-24 | 2016-05-24 | A kind of particle gun piston specimen holder |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262323A (en) * | 1999-12-11 | 2000-08-09 | 周芳 | High-pressure gas gene gun |
| CN2469017Y (en) * | 2001-02-27 | 2002-01-02 | 周芳 | Desk type gas gene gun |
| EP0690732B1 (en) * | 1994-01-21 | 2003-01-29 | Powderject Vaccines, Inc. | Gas driven gene delivery instrument |
| CN1896217A (en) * | 2006-06-02 | 2007-01-17 | 周芳 | Liquid gene gun |
| CN205710709U (en) * | 2016-05-24 | 2016-11-23 | 中国农业科学院棉花研究所 | Particle gun piston specimen holder |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2130459T3 (en) * | 1994-03-11 | 1999-07-01 | Bayer Ag | AROMATIC POLYCARBONATES WITH POLYOLEFINIC CETOCARBOXYL TERMINAL GROUPS, MIXED WITH KNOWN POLYCARBONATES. |
-
2016
- 2016-05-24 CN CN201610349894.4A patent/CN105820942B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0690732B1 (en) * | 1994-01-21 | 2003-01-29 | Powderject Vaccines, Inc. | Gas driven gene delivery instrument |
| CN1112943C (en) * | 1994-01-21 | 2003-07-02 | 粉剂注射疫苗股份有限公司 | Gas driven gene delivery instrument |
| CN1262323A (en) * | 1999-12-11 | 2000-08-09 | 周芳 | High-pressure gas gene gun |
| CN2469017Y (en) * | 2001-02-27 | 2002-01-02 | 周芳 | Desk type gas gene gun |
| CN1896217A (en) * | 2006-06-02 | 2007-01-17 | 周芳 | Liquid gene gun |
| CN205710709U (en) * | 2016-05-24 | 2016-11-23 | 中国农业科学院棉花研究所 | Particle gun piston specimen holder |
Non-Patent Citations (1)
| Title |
|---|
| "A gas-driven gene gun for microprojectile methods of genetic engineering";S M Nabulsi等;《Meas.Sci.Technol》;19941231;第5卷(第3期);第267-274页 * |
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| Publication number | Publication date |
|---|---|
| CN105820942A (en) | 2016-08-03 |
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