CN105820942A - Piston sample rack for gene gun - Google Patents
Piston sample rack for gene gun Download PDFInfo
- Publication number
- CN105820942A CN105820942A CN201610349894.4A CN201610349894A CN105820942A CN 105820942 A CN105820942 A CN 105820942A CN 201610349894 A CN201610349894 A CN 201610349894A CN 105820942 A CN105820942 A CN 105820942A
- Authority
- CN
- China
- Prior art keywords
- piston
- cylinder
- cup
- particle gun
- specimen holder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Strength Of Materials By Application Of Mechanical Stress (AREA)
Abstract
The invention relates to a piston sample rack for a gene gun. The piston sample rack is characterized by comprising a vertical cylinder with the two ends open; a piston is arranged on the inner wall of the cylinder in a sliding mode, and the piston is connected with a cup used for containing an acceptor material through a plurality of supporting rods arranged at intervals. A slide with holes is arranged at the bottom of the cup, and a frosted surface is arranged on the inner side of the slide. A plurality of through holes are formed in the part, above the cup, of the side wall of the cylinder. Sliding ways are arranged on the inner wall of the cylinder, and the piston and the sliding ways are connected in a sliding mode. A gap is formed between the cylinder and the cup. A supporting frame is arranged on the outer wall of the lower portion of the cylinder. A stretching-out end is arranged at the bottom of each sliding way. Scale mark values are arranged on the outer wall of the cylinder. By means of the piston sample rack, gas flow impact to the acceptor material can be relieved, the acceptor material is prevented from being blown off, and thus the hit efficiency of the gene gun is improved; the piston sample rack is simple in structure and convenient to use.
Description
Technical field
The present invention relates to a kind of specimen holder, especially with regard to a kind of particle gun piston specimen holder.
Background technology
Particle bombardment is a kind of brand-new gene Transfection Technology, micro-for the high speed being attached with hereditary material or other material bullet is emitted directly toward cell, tissue and organelle, it is the most state-of-the-art current gene Transfection Technology, there is quick, easy, safe and efficient feature.The gas shock wave that gas gene gun changes into compressed gas (helium or nitrogen) is as power, and the gas shock wave of the one " cold " produced by gas gene gun sends into bombardment room, thus avoids the cell injury that " hot " gas shock wave causes.Gas gene gun can obtain instantaneous, stable and high efficiency transformation in cellular type widely.Gas gene gun has a special construction producing shock wave, has the most different rupturable films from 3.5～10MPa in appropriate air pressure range, is passed through by the particle of cladding DNA, finally injects in the target cell bottom bombardment room.Particle gun is applicable to the transgenic of animals and plants, cell culture, embryo, antibacterial and meiofauna.
In prior art, when utilizing the powder acceptor materials such as Bombardment-Mediated Transformation pollen, major part experimenter is that pollen is laid on glass dish or is positioned over airtight sample room, is placed under particle gun bombardment.During the genetic transformation with millet mature pollen as carrier such as Dong Yunzhou, taking the mature pollen of Ji Gu 11, after sieving, going the removal of impurity, be laid on glass dish by clean pollen, be finally placed under particle gun bombardment, bombard complete, horse back brush pen picks pollen.During it addition, Liu Danmei etc. utilize biolistic bombardment mature flower powder maize transformation, first sample is put into sample room, then add stop net.But, when utilizing glass dish or airtight sample room to convert the dusty materials such as pollen, after particle gun is shot, the biggest air-flow can be produced.Powder acceptor material is little due to light weight, volume, after powerful gas shock, is easily dispelled, therefore have impact on the hit efficiency of particle gun.
Summary of the invention
For the problems referred to above, it is an object of the invention to provide the particle gun piston specimen holder that a kind of hit efficiency is high and easy to use.
For achieving the above object, the present invention takes techniques below scheme: a kind of particle gun piston specimen holder, it is characterized in that: it includes a both ends open and the cylinder of vertical layout, sliding on the inwall of described cylinder and arrange a piston, described piston connects one for holding the cup of acceptor material by multiple spaced apart support bars.
The bottom of described cup is arranged to slide glass with holes, is arranged to frosting in the inner side of described slide glass.
Described cylinder is positioned on the sidewall above described cup and offers multiple through hole.
Being provided with slideway on the inwall of described cylinder, described piston is slidably connected with described slideway.
It is provided with gap between described cylinder and described cup.
The outer wall of described cylinder bottom is provided with bracing frame.
The bottom of described slideway is provided with external part.
The outer wall of described cylinder is provided with scale value.
Due to the fact that and take above technical scheme, it has the advantage that 1, the present invention is provided with a cylinder, and sliding on the inwall of cylinder arranges a piston, in order to piston moves down along cylinder inboard wall.2, inventive piston connects a cup by multiple support bars, when piston is moved down by gas shock, drives cup to move down, reduces the gas shock of acceptor material such that it is able to increase particle gun hits rate.3, present invention gas in the bottom of cup is arranged to slide glass with holes, beneficially cylinder is discharged, it is possible to prevent the gas rapid expanding that particle gun is shot in rear cylinder body.4, the present invention is positioned on the sidewall above cup the gas discharge offering in multiple through hole, beneficially cylinder on cylinder, it is possible to prevent the gas rapid expanding that particle gun is shot in rear cylinder body.5, the inner side of slide glass of the present invention is arranged to frosting, by increasing capacitance it is possible to increase the frictional force between slide glass and cup, makes powder acceptor material keep geo-stationary in cup.6, the gas in the present invention is provided with gap, beneficially cylinder between cylinder and cup is discharged, it is also possible to prevent the gas rapid expanding that particle gun is shot in rear cylinder body.7, the present invention is provided with scale value on the outer wall of cylinder, for observing the cup position in inner barrel, in order to adjust the distance between particle gun and cup.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention
Fig. 2 is the schematic top plan view after the present invention removes bracing frame
Detailed description of the invention
With embodiment, the present invention is described in detail below in conjunction with the accompanying drawings.
As shown in Figure 1 and Figure 2, the present invention proposes a kind of particle gun piston specimen holder, it includes the cylinder 1 of a both ends open and vertical layout, and sliding on the inwall of cylinder 1 arranges a piston 2, and piston 2 connects one for holding the cup 4 of acceptor material by multiple spaced apart support bars 3.
In above-described embodiment, as it is shown in figure 1, be arranged to the slide glass 6 of with holes 5 in the bottom of cup 4, the aperture in hole 5 is discharged less than the gas in acceptor material, beneficially cup 4, it is to avoid particle gun 7 is shot the air rapid expanding in rear cylinder body 1.Being arranged to frosting in the inner side of slide glass 6, by increasing capacitance it is possible to increase the frictional force between acceptor material and slide glass 6, so, when cup 4 moves, acceptor material can keep geo-stationary in cup 4.
In above-described embodiment, discharge as it is shown in figure 1, be positioned on the sidewall above cup 4 gas offering in multiple through hole 8, beneficially cylinder 1 on cylinder 1.
In above-described embodiment, as it is shown in figure 1, be provided with slideway 9 on the inwall of cylinder 1, piston 2 is slidably connected with slideway 9.
In above-described embodiment, as it is shown in figure 1, be provided with gap between cylinder 1 and cup 4, the beneficially gas in cylinder 1 is discharged.
In above-described embodiment, as it is shown in figure 1, be provided with bracing frame 10 on the outer wall of cylinder 1 bottom, in order to realize cylinder 1 unsettled.
In above-described embodiment, as it is shown in figure 1, external part 11 can be provided with in the bottom of slideway 9, after particle gun 7 is shot, after cup 4 moves down under the effect of air-flow, it is used for supporting support bar 3.
In above-described embodiment, the outer wall of cylinder 1 is provided with scale value, for observing cup 4 in the position within cylinder 1, in order to adjust the distance between particle gun 7 and cup 4.
In above-described embodiment, as it is shown in figure 1, a diameter of 7～9 centimetres of cylinder 1, a height of 16～20 centimetres.
In above-described embodiment, as it is shown in figure 1, a diameter of 3.5～4.5 centimetres of cup 4, a height of 3.5～4.5 centimetres.Wherein, the particle gun 7 bombardment scope radius within 14 centimetres of ranges is not more than 1.7 centimetres, owing to particle gun bombardment scope radius within 14 centimetres of ranges is not more than 1.7 centimetres, so the diameter design of cup 4 is 3.5～4.5 centimetres.
In above-described embodiment, as shown in Figure 1 and Figure 2, piston 2 uses elastomeric material to make.
During as it is shown in figure 1, the present invention uses, first acceptor material is placed in cup 4.When carrying out genetic transformation, after utilizing particle gun 7 to shoot, in cup 4, produce the biggest air-flow.Under the effect of air-flow, it is possible to promote piston 2 to move down along slideway 9, to reduce the gas shock to acceptor material, prevent acceptor material from being dispelled, thus increase the hit efficiency of particle gun 7.
The various embodiments described above are merely to illustrate the present invention, and the structure of the most each parts, connected mode etc. all can be varied from, and every equivalents carried out on the basis of technical solution of the present invention and improvement the most should not be got rid of outside protection scope of the present invention.
Claims (8)
1. a particle gun piston specimen holder, it is characterized in that: it includes a both ends open and the cylinder of vertical layout, sliding on the inwall of described cylinder and arrange a piston, described piston connects one for holding the cup of acceptor material by multiple spaced apart support bars.
2. a kind of particle gun piston specimen holder as claimed in claim 1, it is characterised in that: the bottom of described cup is arranged to slide glass with holes, is arranged to frosting in the inner side of described slide glass.
3. a kind of particle gun piston specimen holder as claimed in claim 1, it is characterised in that: it is positioned on described cylinder on the sidewall above described cup and offers multiple through hole.
4. a kind of particle gun piston specimen holder as claimed in claim 1, it is characterised in that: being provided with slideway on the inwall of described cylinder, described piston is slidably connected with described slideway.
5. a kind of particle gun piston specimen holder as claimed in claim 1, it is characterised in that: between described cylinder and described cup, it is provided with gap.
6. a kind of particle gun piston specimen holder as claimed in claim 1, it is characterised in that: on the outer wall of described cylinder bottom, it is provided with bracing frame.
7. a kind of particle gun piston specimen holder as claimed in claim 1, it is characterised in that: the bottom of described slideway is provided with external part.
8. a kind of particle gun piston specimen holder as claimed in claim 1, it is characterised in that: on the outer wall of described cylinder, it is provided with scale value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610349894.4A CN105820942B (en) | 2016-05-24 | 2016-05-24 | A kind of particle gun piston specimen holder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610349894.4A CN105820942B (en) | 2016-05-24 | 2016-05-24 | A kind of particle gun piston specimen holder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105820942A true CN105820942A (en) | 2016-08-03 |
| CN105820942B CN105820942B (en) | 2017-12-19 |
Family
ID=56530542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610349894.4A Active CN105820942B (en) | 2016-05-24 | 2016-05-24 | A kind of particle gun piston specimen holder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105820942B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112943A (en) * | 1994-03-11 | 1995-12-06 | 拜尔公司 | Polycarbonates with aliphatic ketocarboxyl end groups, mixed with known aromatic polycarbonates their preparation and their use |
| EP0690732A1 (en) * | 1994-01-21 | 1996-01-10 | Auragen, Inc. | Gas driven gene delivery instrument |
| CN1262323A (en) * | 1999-12-11 | 2000-08-09 | 周芳 | High-pressure gas gene gun |
| CN2469017Y (en) * | 2001-02-27 | 2002-01-02 | 周芳 | Desk type gas gene gun |
| CN1896217A (en) * | 2006-06-02 | 2007-01-17 | 周芳 | Liquid gene gun |
| CN205710709U (en) * | 2016-05-24 | 2016-11-23 | 中国农业科学院棉花研究所 | Particle gun piston specimen holder |
-
2016
- 2016-05-24 CN CN201610349894.4A patent/CN105820942B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0690732A1 (en) * | 1994-01-21 | 1996-01-10 | Auragen, Inc. | Gas driven gene delivery instrument |
| CN1112943A (en) * | 1994-03-11 | 1995-12-06 | 拜尔公司 | Polycarbonates with aliphatic ketocarboxyl end groups, mixed with known aromatic polycarbonates their preparation and their use |
| CN1262323A (en) * | 1999-12-11 | 2000-08-09 | 周芳 | High-pressure gas gene gun |
| CN2469017Y (en) * | 2001-02-27 | 2002-01-02 | 周芳 | Desk type gas gene gun |
| CN1896217A (en) * | 2006-06-02 | 2007-01-17 | 周芳 | Liquid gene gun |
| CN205710709U (en) * | 2016-05-24 | 2016-11-23 | 中国农业科学院棉花研究所 | Particle gun piston specimen holder |
Non-Patent Citations (1)
| Title |
|---|
| S M NABULSI等: ""A gas-driven gene gun for microprojectile methods of genetic engineering"", 《MEAS.SCI.TECHNOL》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105820942B (en) | 2017-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102826060B (en) | Bionic energy absorption pipe of bamboo-like structure | |
| CN205710709U (en) | Particle gun piston specimen holder | |
| CN205574291U (en) | Double -deck aircraft protection device | |
| CN105820942A (en) | Piston sample rack for gene gun | |
| CN205831666U (en) | A kind of oven thermal insulation support | |
| CN208882105U (en) | A kind of multi-purpose unmanned aerial vehicle undercarriage | |
| CN207480561U (en) | A kind of rack convenient for placement mobile phone injection mold | |
| CN209023522U (en) | A kind of cooling storage tank device | |
| CN208526678U (en) | A kind of control water bracket for bio-pharmaceuticals | |
| CN208261259U (en) | A kind of tealeaves multistage pneumatic separation screening plant | |
| CN110498112A (en) | A kind of cell transporting equipment | |
| CN202764894U (en) | Bionic energy absorption tube imitating bamboo structure | |
| CN202947453U (en) | Ventilating type spice drying box spice-placing frame | |
| CN212113851U (en) | New energy automobile battery is with placing case | |
| CN207783498U (en) | A kind of capsicum seedling transplanting apparatus | |
| CN208903665U (en) | A kind of portable computer hard disk installation structure | |
| CN208972266U (en) | A kind of livestock rearing draft fan | |
| CN209696230U (en) | A kind of transmitter installation for football training | |
| CN208326369U (en) | A kind of ganoderma lucidum storing unit | |
| CN204258139U (en) | Backpack type portable electric pay off rack | |
| CN215004308U (en) | Quick framework device of test equipment of aviation simulation mechanism | |
| CN212870191U (en) | High-stability air purifier base for cabin | |
| CN221058852U (en) | Device for improving picking efficiency of dragon fruits | |
| CN208560167U (en) | A kind of glass curtain wall guard rail structure | |
| CN210285319U (en) | Liquid nitrogen tank and sample bearing device thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |