CN1896217A

CN1896217A - Liquid gene gun

Info

Publication number: CN1896217A
Application number: CNA2006100517831A
Authority: CN
Inventors: 周芳
Original assignee: Individual
Current assignee: Individual
Priority date: 2006-06-02
Filing date: 2006-06-02
Publication date: 2007-01-17

Abstract

A liquid gene sprayer consists of casing (1) and magazine (6) connected with casing (1). The magazine (6) comprises hollow cylinder (62) and sliding piston rod set in it, emission hole (62b) is arranged at front end of cylinder (62), front end of casing (1) is fixed connected to rear end opening, impeller (3) moving forwardly and backwardly and energy-storage driving mechanism are set in internal cavity of casing (1), and trigger in energy-storage state can make impeller (3) limit on casing (1) rear. It is safe, efficient and convenient, adopts small-volume energy-storage driver and is no need for gas source equipments.

Description

A kind of liquid gene gun

Technical field

The present invention relates to a kind ofly genetic material or other materials be injected the instrument of cell, tissue or organoid, particularly a kind of liquid gene gun that adopts liquid medium in bio-genetics.

Background technology

In the bio-genetics field, particle gun is a kind of requisite instrument, initial particle gun is to adopt the gunpowder bullet to bombard, as the patent No. is that 89109334 Chinese invention patent " a kind of method of metastatic gene and the particle gun of metastatic gene thereof " discloses the particle gun so a kind of, its utilizes " speed and the method for the proportion speed ground control bombardment of gunpowder; and can operate under the closed state He under the non-closed state; can be to bombarding the importing of realization foreign gene from biomass cells, thereby the proterties of change biology with task equipment." this genoid rifle can damage to some extent to sample, the impurity in the gunpowder also can be injected in the sample simultaneously, and its precision often is difficult to control.

In view of the foregoing, gas percussion ripple that pressurized gas converts to the particle gun as propulsion source has appearred utilizing, as disclosed among U.S. Pat 5865796, the US558480 be exactly a kind of pistol type particle gun.During use, inlet suction port is connected high-pressure air source, when pressure is enough, opens air valve, and pressurized gas enters gun barrel rapidly and forms a gas percussion ripple, and the DNA particulate that is arranged in the magazine is entered target from the muzzle ejaculation.Its shortcoming is that the magazine seat only depends on the steady brace location, and is stable inadequately, and the vibrations that previous fire is brought can influence as yet the not homogeneity of the DNA particulate of emission, and simultaneously, the operation of conversion magazine is also convenient inadequately.The pressurized gas that is used on the other hand launching the DNA particulate does not pass through the gas storage stable-pressure device directly by the compressed gas source importing, so not only because of the gas circuit pressure-losses influences stable gas pressure, but also can influence the initial velocity of launching the DNA particulate.Yet also there is following defective in such scheme in practice process: 1. adopt compressed gas source, need insert compressed pneumatic pump during use, volume is big, carries inconvenience.2. with pressurized gas as propulsion source, fltting speed is limited, can not reach the high-velocity jet of DNA bullet.3. because bullet and link stopper are rigid impacts, for preventing dispersing of DNA particulate, the DNA microparticle shell of emission is surrounded by gold grain, on the other hand, the DNA particulate is after emission, and gold grain can bring certain negative effect to organism itself along with the DNA particulate enters in biological cell, the organizer.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of liquid gene gun that need not external compressed gas source at above-mentioned prior art present situation.

The present invention solves the problems of the technologies described above the technical scheme that is adopted: this liquid gene gun comprises housing with inner chamber and the magazine that is connected with housing, it is characterized in that described magazine comprises the cylindrical shell of hollow and is slidably disposed on its interior piston rod, the front end of cylindrical shell has transmitting aperture, open rearward end also is fixedly connected on the front end of housing, in the inner chamber of housing, be provided with an impeller that can move forward and backward and make the fast accumulation of energy driving mechanism of reach of impeller, and include one and make that impeller is spacing to make it be in the trigger of energy accumulating state in the housing rear positions.

Described accumulation of energy driving mechanism can adopt various prior aries, and also can adopting one simply, to be positioned at the intravital spring of shell be main element, and its front end and impeller offset, and the afterbody of its rear end and housing offsets.In order to make this spring location more stable, described impeller is good to have the longitudinal section that is the T type, and its afterbody penetrates described spring, and the spring front end promptly withstands on the step of impeller head; Simultaneously, described housing afterbody also can be by a plug sealing that is connected with the housing thread in inner cavity, and this plug front end has a central boss, and described spring afterbody promptly is enclosed within outside this central boss.

Described trigger can adopt various prior aries, but preferably, described trigger runs through the medium position that the bearing pin of portion wherein is arranged on housing rotationally by one, the rear portion of trigger has a part bending down, this kink can insert housing in the peristome of corresponding position, and is stuck on the front end of the impeller that is in energy accumulating state.Use for convenience, improve security, the front portion of described trigger can also be provided with a return spring, and the lower end and the housing of spring offset, and the upper end and the trigger of spring offset.For convenient on making and be convenient to the flexible rotation of trigger, described trigger can comprise pressing plate and baffle plate, wherein baffle plate is provided with in the concave belly of pressing plate loosely, described trigger kink promptly is arranged on the baffle plate, and described housing has two parallel wing plates that upwarp in the corresponding position, promptly between these two wing plates, described bearing pin promptly laterally runs through the medium position of pressing plate and baffle plate for described pressing plate and baffle plate, and the bearing pin two ends are separately fixed on the described housing wing plate.

In order further to strengthen security, prevent to mistakenly launch, also can be arranged with rotating pivoted housing in described housing front portion, and, the afterbody of pivoted housing is cam-like, and is positioned under the front portion of trigger, and trigger then has in the corresponding position can hold this cam edge embedding groove wherein.

For the ease of magazine is installed on the housing, the periphery of described magazine cylindrical shell also can have the radially hand-tight lug of evagination.

In order to make the DNA gene have certain emtting area, the transmitting aperture of described barrel front end with tapered horn-like be good, the opening of described tapered hole is along the cylindrical shell centerline direction, the maximum position opening of tapered hole is near emitting facet.

Compared with prior art, the invention has the advantages that by the built-in accumulation of energy drive mechanism sliding shoe of housing to move forward fast that the propulsion source as promoting the cylindrical shell piston rod movement need not to dispose in addition air pump, reduced the volume, easy to carry of particle gun.On the other hand, can inject the liquid solvent that carries DNA in the cylindrical shell, when injecting sample or entity sample, need not to coat gold grain, reduce hemorrhage, thereby use more safe and effective.

Description of drawings

Fig. 1 is the structure cross-sectional schematic of the embodiment of the invention.

Fig. 2 is the structure cross-sectional schematic of the embodiment of the invention under state after the emission.

Fig. 3 is the right view of Fig. 1.

Fig. 4 is the A-A sectional view of Fig. 1.

Fig. 5 is the B-B sectional view of Fig. 1.

Embodiment

Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.

As Fig. 1～shown in Figure 2, the cylindrical shell 62 that liquid gene gun includes housing 1 and is threaded with housing, housing 1 afterbody has a bonnet that is threaded with housing 11, and the front end center projections 11a of bonnet 11; A T type slide bar 3 that can move forward and backward is arranged in the housing 1, its afterbody is positioned within the housing 1, have a stage clip 2 to be positioned at housing 1 inner chamber, of stage clip 2 is fixedly connected on the center projections 11a of bonnet 11 front ends, and the other end of stage clip 2 is connected on the T type cascaded surface of slide bar 3;

One pull bar 61 is slidably arranged in the cylindrical shell 62, and pull bar 61 heads have the rubber coating envelope 61a of a sealing usefulness, and the shift motion of pull bar 61 in cylindrical shell 6 is 5～50 millimeters; The periphery of cylindrical shell 62 is evenly distributed with four and is the radially hand-tight lug 62a of evagination, and it is near cylindrical shell 62 afterbodys, referring to Fig. 3; The front end of cylindrical shell 62 has transmitting aperture, and is tapered horn-like, and the opening of tapered hole 62b is along the cylindrical shell centerline direction, and the maximum position opening of tapered hole 62b is near emitting facet; Wherein, the diameter range in the hole, minimum position of conical horn hole 62b is 30～70 microns, and the diameter range in hole, maximum position is 120～200 microns, referring to Fig. 1 or Fig. 2;

Outside housing 1, also be provided with a spanner 4, this spanner 4 comprises pressing plate 43 and baffle plate 41, wherein baffle plate 41 is an i section and is provided with loosely in the concave belly of pressing plate 43, housing 1 has two parallel wing plates 45 that upwarp in the corresponding position, pressing plate 43 and baffle plate 41 are promptly between these two wing plates 45, one steady brace 42 laterally runs through the medium position of pressing plate 43 and baffle plate 41, and steady brace 42 two ends are separately fixed on the housing 1 wing plate 45, referring to Fig. 4; The rear portion of baffle plate 41 has the bending pin 41a of a downward bending structure, and this bending pin 41a can insert housing 1 in the opening of corresponding position, and is stuck on the T type stagewise front end of the slide bar 3 that is in energy accumulating state.Be provided with in the front portion of spanner 4 and also have a little spring 44 that resets usefulness, the lower end of little spring 44 and housing 1 offset, and the upper end of little spring 44 and spanner 4 offset;

Housing 1 front portion is arranged with a cam condom 5, itself and housing 1 usefulness one screw 51 are fixed in housing 1 periphery, and screw 51 heads and housing 1 leave and rotate the slit, cam condom 5 after promptly connecting can rotate along housing 1 periphery, cam condom 5 radially locate the projection that about 3/4ths circumference are colyliform, referring to Fig. 5, its position faces the groove under the anterior pressing plate of spanner, and this groove can hold cam edge embeds wherein it;

During use, earlier cylindrical shell 62 is taken off together with pull bar 61, housing 1 opening end is inserted reset little bar on the box of a special use, firmly close,, when bending pin 41a blocks the T type end side of slide bar 3, have chucking sound to compress stage clip 2, stage clip 2 reaches the preceding energy accumulating state of work by pretension at this moment; Cylindrical shell 62 heads are inserted in the solvent of fusion dna particulate, outwards slowly extract pull bar, suck the liquid drug with the NDA particulate, then cylindrical shell 62 is screwed on the housing 1, at this moment, particle gun is in the preceding state of work, referring to Fig. 1; When particle gun is worked, elder generation's rotating cam condom 5 is to depart from the range comprehension of spanner 4 pressing plate grooves, depress spanner 4, this moment, the bending pin 41a at baffle plate 41 rear portions no longer blocked the front end face of T type slide bar 3, and stage clip 2 drives slide bars 3 fast transferrings, and the preceding release of pull bar 61a of push up mutually of promotion and slide bar 3, the conical horn mouth 62b ejaculation of the liquid particulate of DNA from cylindrical shell 62 will be carried, inject in sample or the entity sample, finish a DNA and import, referring to Fig. 2; The emission rate of particle gun can reach 200～500m/s, and emission depth reaches 0.5～8mm, when a DNA inject finish after, only need turn on and be threaded separately cylindrical shell 62 and housing 1, repeat aforesaid operations, can carry out work next time.

Claims

1, a kind of liquid gene gun, include housing (1) with inner chamber and the magazine that is connected with housing (6), it is characterized in that described magazine (6) comprises the cylindrical shell (62) of hollow and is slidably disposed on its interior piston rod (61), the front end of cylindrical shell (62) has transmitting aperture (62b), open rearward end also is fixedly connected on the front end of housing (1), in the inner chamber of housing (1), be provided with an impeller that can move forward and backward (3) and make the fast accumulation of energy driving mechanism of reach of impeller (3), and include one and make that impeller (3) is spacing to make its trigger that is in energy accumulating state (4) in housing (1) rear positions.

2, liquid gene gun according to claim 1 is characterized in that it is main element that described accumulation of energy driving mechanism is positioned at the intravital spring of shell (2) with one, and its front end and impeller (3) offset, and the afterbody of its rear end and housing (1) offsets.

3, liquid gene gun according to claim 2 is characterized in that described impeller (3) has the longitudinal section that is the T type, and its afterbody penetrates described spring (2), and spring (2) front end promptly withstands on the step of impeller (3) head.

4, liquid gene gun according to claim 2, it is characterized in that described housing (1) afterbody is by plug (a 11) sealing that is connected with housing (1) thread in inner cavity, this plug (11) front end has a central boss (11a), and described spring (2) afterbody promptly is enclosed within outside this central boss (11a).

5, liquid gene gun according to claim 1, it is characterized in that described trigger (4) runs through the medium position that the bearing pin of portion (42) wherein is arranged on housing (1) rotationally by one, the rear portion of trigger (4) has a part bending down (41a), this kink (41a) can insert housing (1) in the peristome of corresponding position, and is stuck on the front end of the impeller (3) that is in energy accumulating state.

6, liquid gene gun according to claim 5 is characterized in that being provided with a return spring (44) in the front portion of described trigger (4), and the lower end of spring (44) and housing (1) offset, and the upper end of spring (44) and trigger (4) offset.

7, liquid gene gun according to claim 5, it is characterized in that described trigger (4) comprises pressing plate (43) and baffle plate (41), wherein baffle plate (41) is provided with in the concave belly of pressing plate (43) loosely, described trigger kink (41a) promptly is arranged on the baffle plate (41), and described housing (1) has two parallel wing plates (45) that upwarp in the corresponding position, described pressing plate (43) and baffle plate (41) promptly are positioned between these two wing plates (45), described bearing pin (42) promptly laterally runs through the medium position of pressing plate (43) and baffle plate (41), and the two ends of bearing pin (42) are separately fixed on the described housing wing plate (45).

8, liquid gene gun according to claim 5, it is characterized in that described housing (1) front portion also is arranged with rotating pivoted housing (5), and, the afterbody of pivoted housing (5) is cam-like, and be positioned under the front portion of trigger (4), trigger (4) then has in the corresponding position can hold this cam edge embedding groove wherein.

9,, it is characterized in that magazine cylindrical shell (62) periphery also has the radially hand-tight lug (62a) of evagination according to the arbitrary described liquid gene gun of claim 1 to 8.

10, according to the arbitrary described liquid gene gun of claim 1 to 8, the transmitting aperture (62b) that it is characterized in that described cylindrical shell (62) front end is tapered horn-like, described tapered hole opening is along cylindrical shell (62) centerline direction, and the maximum position opening of tapered hole is near emitting facet.