CN105795505A - Tobacco additive prepared from fresh tobacco leaves as well as preparation method and application of tobacco additive - Google Patents
Tobacco additive prepared from fresh tobacco leaves as well as preparation method and application of tobacco additive Download PDFInfo
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- CN105795505A CN105795505A CN201610335661.9A CN201610335661A CN105795505A CN 105795505 A CN105795505 A CN 105795505A CN 201610335661 A CN201610335661 A CN 201610335661A CN 105795505 A CN105795505 A CN 105795505A
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- additive agent
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- tobacco leaves
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Manufacture Of Tobacco Products (AREA)
Abstract
The invention relates to a tobacco additive prepared from fresh tobacco leaves as well as a preparation method and application of the tobacco additive, and belongs to the technical field of cigarette essence and spices. The method comprises the following steps: picking ripe middle tobacco leaves; washing the surfaces of the tobacco leaves and then removing main veins of the tobacco leaves; mixing the tobacco leaves with a phosphate buffer solution at the mass-volume ratio of 1 to 12; crushing an obtained biological mixture at 4 to 10 DEG C through a crusher; filtering the crushed mixture through a sterile gauze and removing filtered residues; filtering and concentrating filtrate with a hollow fiber column with the size of 0.22 micron; naturally depositing a concentrate at 4 DEG C to form three layers; uniformly mixing a middle-layer substance and a lowest-layer substance at the mass ratio of (2:1) to (1:2) to obtain the product. The preparation method is simple and the prepared additive is safe and pollution-free; any chemical substance, which is harmful to human bodies, does not need to be added; the additive does not weaken the fragrance when the content of tar of cigarettes is reduced, and is easy to popularize and apply.
Description
Technical field
The invention belongs to technical field of cigarette flavors, be specifically related to a kind of tobacco additive agent coming from fresh tobacco leaves and
Its preparation method and application.
Background technology
China is a tobacco industry big country, and tobacco planting area and yield all occupy first place in the world, and the sales volume Ye Zhan world is first
Position, tobacco planting plays an important role in national economy with producing.Nicotiana tabacum L. is coal-tar middle oil as you know waits material to be harmful to
Health, but smoking has become a part for people's life, is difficult to change, the needs of simultaneous economic development, people
Research in terms of starting to accept smoking and emphasis having been turned to how reducing tar and reducing harm, early oneself is not difficult matter to reduce tar content, but
It is that a large amount of fragrance matters contained in Nicotiana tabacum L. also greatly reduce while reducing tar, and this certainly will cause smoke the lightest and cigarette
Perfume is not enough, cannot meet the hobby of consumer.How while reducing tar content, to accomplish not weaken fragrance, do not destroy fragrance
Style, and make Medicated cigarette reach one of preferable stability difficult problem becoming tobacco industry, add flavouring essence for tobacco and alcoholized tobacco
This defect can be compensate for.
Flavouring essence for tobacco as a kind of special food additive, current essence be divided into chemical synthesis prepare flavouring essence for tobacco,
Plant extract prepares flavouring essence for tobacco, biotechnology prepares flavouring essence for tobacco and the raw fragrant technology of U.S. rad prepares flavouring essence for tobacco.Mei La
Moral reaction be formed Nicotiana tabacum L. characteristic chicken flavor main by way of one of, Nicotiana tabacum L. is during modulating, be aged, process and burning and sucking, all
Along with non enzymatic browning reacton in various degree, produce many flavor components.Maillard reaction institute is assert by internal authority mechanism
The essence base of preparation is " natural ", and is widely adopted in novel flavouring essence for tobacco, and starch is at Nicotiana tabacum L. internal starch enzyme
It is hydrolyzed into the saccharide such as glucose, maltose under effect, fragrance thing can be generated with tobacco internal aminoacid generation Maillard reaction
Matter maillard reaction product, makes Medicated cigarette fragrance matter content increase.
Alcoholization is the important means improving cigarette quality generally used at present, and tobacco mellowing important mechanisms is
The mechanism of enzyme.Owing to the endogenous enzymes kind in flue-cured tobacco and content are the most fewer, it is impossible to make Nicotiana tabacum L. obtain at short notice
To sufficiently alcoholization, therefore purification cycle length, take storage area and human resources is more, use fresh tobacco leaves extracts and add
Containing a large amount of sources enzyme in agent, process the Nicotiana tabacum L. alcoholization process with promotion flue-cured tobacco, to improving quality of tobacco further.So such as
What overcomes the deficiencies in the prior art to be the problem that current technical field of cigarette flavors needs solution badly.
Summary of the invention
The invention aims to solve the deficiencies in the prior art, it is provided that a kind of tobacco additive agent coming from fresh tobacco leaves
And preparation method and application.Preparation method of the present invention is simple, the additive safety non-pollution prepared, it is not necessary to add any to people
The chemical substance that body is harmful, and this additive is so that accomplish not weaken fragrance, easily while Medicated cigarette reduces tar content
In popularization and application.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of preparation method of the tobacco additive agent coming from fresh tobacco leaves, including as follows:
Nicotiana tabacum L. in the middle part of harvesting ripe, will pump Nicotiana tabacum L. master pulse, then will pump Nicotiana tabacum L. and the temperature of master pulse after tobacco leaf surface wash clean
The phosphate buffer that degree is 4-10 DEG C mixes according to mass volume ratio 1:4-12, obtains mixture；Described phosphate buffer pH
Value is 7, and concentration is 0.01M-0.05M；
Mixture is at 4-10 DEG C afterwards, carries out being crushed to crushing residua diameter less than 2mm by disintegrating machine；
Mixture after Po Sui filters and discards residue by sterile gauze, filtrate by 0.22um hollow fiber column filtering and concentrating,
It is concentrated into the 18-22% that volume is filtrate volume after the 18-22%(of original volume i.e. concentrates)；
Then concentrate carries out natural subsidence to being divided into 3 layers at 3-8 DEG C, takes intermediate layer material and orlop material according to mass ratio
For 2:1 ~ 1:2 mix homogeneously, i.e. obtain coming from the tobacco additive agent of fresh tobacco leaves.
It is further preferred that the preparation method of the described tobacco additive agent coming from fresh tobacco leaves, including by following step
Rapid:
Nicotiana tabacum L. in the middle part of harvesting ripe, will pump Nicotiana tabacum L. master pulse, then will pump Nicotiana tabacum L. and the temperature of master pulse after tobacco leaf surface wash clean
Degree is that the phosphate buffer of 4 DEG C mixes according to mass volume ratio 1:12, obtains mixture；Described phosphate buffer pH value is 7,
Concentration is 0.01M；
Mixture is at 4 DEG C afterwards, carries out being crushed to crushing residua diameter less than 2mm by disintegrating machine；
Mixture after Po Sui filters and discards residue by sterile gauze, filtrate by 0.22um hollow fiber column filtering and concentrating,
It is concentrated into the 20% of original volume；
Then concentrate carries out natural subsidence at 4 DEG C, carries out natural subsidence to being divided into 3 layers, takes intermediate layer material and orlop thing
Matter is 1:1 mix homogeneously according to mass ratio, i.e. obtains coming from the tobacco additive agent of fresh tobacco leaves.
During it is further preferred that described employing sterile gauze filters, sterile gauze is of five storeys.
It is further preferred that described static balance time is more than 5h.
It is further preferred that taking intermediate layer material and orlop material is 1:1 mix homogeneously according to mass ratio, i.e. obtain
Come from the tobacco additive agent of fresh tobacco leaves.
It is further preferred that after the tobacco additive agent subpackage of fresh tobacco leaves being come from, seal in 4 DEG C and preserve.
The present invention also provide for a kind of above-mentioned tobacco additive agent coming from fresh tobacco leaves preparation method prepare come from fresh
The tobacco additive agent of Nicotiana tabacum L..
The tobacco additive agent coming from fresh tobacco leaves that the present invention also provides for a kind of above-mentioned preparation method prepared is preparing Medicated cigarette
In application, be that the tobacco additive agent coming from fresh tobacco leaves is added in the tobacco shred of low-coke tar cigarette, according to conventional preparation
Technique is prepared as Medicated cigarette；
Addition is the 0.004%-0.3% of low-coke tar cigarette tobacco shred weight.
It is further preferred that the addition coming from the tobacco additive agent of fresh tobacco leaves is low-coke tar cigarette tobacco shred weight
0.004%-0.1%.
It is further preferred that after the tobacco additive agent distilled water coming from fresh tobacco leaves is diluted 300-800 times uniformly
Be sprayed on low-coke tar cigarette tobacco shred.
It is further preferred that spray equably after the tobacco additive agent distilled water coming from fresh tobacco leaves is diluted 400 times
Spill on low-coke tar cigarette tobacco shred, after the tobacco shred after sprinkling seals room temperature placement 12h, tobacco shred is put into 90 DEG C of drying bakers and dries
20min；Then the tobacco shred after drying put into temperature be 22 DEG C, relative humidity be 55% climatic chamber in 8-12h balance cigarette
Silk moisture, is finally prepared as Medicated cigarette by the tobacco shred after balance according to conventional preparation technology.
The present invention comes from the concrete steps of the preparation method of the tobacco additive agent of fresh tobacco leaves and preparating mechanism is as follows:
The first step, Nicotiana tabacum L. in the middle part of harvesting ripe, clean silt and other impurity on surface.
Quality of tobacco Divisional: upper smoke, middle part cigarette, bottom cigarette.Upper smoke smoke is dense, and bottom cigarette flue gas is light, is substantially
Inserts, in general middle part cigarette is best in quality, and taste alcohol, smoke are moderate, are suitable for hurrying to the preparation of people's product.Wash away Nicotiana tabacum L.
The silt on surface, silt is mixed into and can produce blue foreign smell and affect cigarette quality, so surface to be rinsed well silt before broken leaf breaking；
Second step, will pump Nicotiana tabacum L. master pulse, is then 4-10 DEG C by the Nicotiana tabacum L. pumping master pulse with temperature after tobacco leaf surface wash clean
Phosphate buffer according to mass volume ratio 1:4-12 mix, obtain mixture；Described phosphate buffer pH value is 7, concentration
For 0.01M-0.05M；Mixture is at 4-10 DEG C afterwards, is crushed by disintegrating machine.
Pumping aorta, because endogenous enzymes contained by aorta and content of starch ratio are relatively low, crude fibre is more, and crushing effect is not
Good, easily entrain impurity, so removing aorta；PH value is 7, concentration is that 0.01M-0.05M phosphate buffer can maintain mesophyll thin
Born of the same parents' normal ph and normal physiological context, resist pH value change, has main component after protection broken leaf breaking；Nicotiana tabacum L. with temperature is
The phosphate buffer 1:4-12 of 4-10 DEG C is mixed with that to help Nicotiana tabacum L. the most broken；Time broken, temperature controls to contribute at 4-10 DEG C
Protection endogenous enzymes will not be because of the too high inactivation of temperature；
3rd step, the mixture after crushing filters and discards residue by 5 layers of sterile gauze, filtered solution 0.22um doughnut
Element post filtering and concentrating, is concentrated into the 18-22% of original volume；
After fresh tobacco leaves is broken, some crude fibre class materials are suspended in mixed liquor, and additive of the present invention is intended to remove these things
Matter, the mixture after crushing discards these residues by aseptic 5 layers of gauze, and (5 layers of gauze aperture are not specifically limited, conventional products
), can fully intercept the scarps such as crude fibre, the most not cause aperture to block；Remove scarp filtered solution contain much starch,
Mesophyll cell, cell debris and the aqueous solution of Water-soluble Organic Substances that some are little, filtered solution is again by 0.22um doughnut
Element post filtering and concentrating, mesophyll cell and starch can not pass through 0.22um hollow cellulose post, moisture, some small molecular weight impurities,
Grain impurity and some water soluble organic substances can pass through 0.22um hollow cellulose post, play the effect that remove impurity concentrates, concentrate
It is mainly composed of starch, mesophyll cell (internal containing a large amount of Nicotiana tabacum L. endogenous enzymes) and cell debris；
4th step, concentrate carries out more than natural subsidence 5h at 4-10 DEG C, carries out starch with mesophyll cell (containing major part Nicotiana tabacum L.
Endogenous enzymes) separate.
Owing to granular size density is different, sedimentation velocity is different, is divided into 3 layers after natural subsidence.Owing to starch density is more than
Mesophyll cell, so orlop is white stratum granulosum during natural subsidence, is that green mesophyll is thin above white stratum granulosum
Born of the same parents' layer, is topmost the sepia phosphoric acid buffer liquid layer containing soluble small molecular.
White stratum granulosum is mainly composed of starch, includes cell debris and other composition composition simultaneously, mixes for creamy white
Compound.
Green mesophyll cellular layer is mainly composed of mesophyll cell, includes cell debris and other composition, enzyme material simultaneously
It is distributed in this layer with vegetable protein major part, for paste green mixture.
After removing the superiors' sepia buffer, lower floor's white stratum granulosum and the green mesophyll cellular layer containing cell debris
Substantially, part cell debris also contains endogenous enzymes and mesophyll cell functional similarity, is hereinafter belonged to mesophyll cell in layering,
Stratum granulosum is separated with mesophyll cell layer；It is owing to low temperature is possible to prevent endogenous enzymes degeneration 4-10 DEG C of natural subsidence；Meanwhile, by
In Nicotiana tabacum L. mesophyll cell and starch nutrition material abundance, and moisture is sufficient, temperature can be prevented too high 4-10 DEG C of natural subsidence and
Generation is gone mouldy；Starch more than natural subsidence 5h just can be made fully to be layered with mesophyll cell.
5th step, respectively extracting waste stratum granulosum and the material of green mesophyll cellular layer, be 2:1 ~ 1:2 mixing according to mass ratio
Uniformly, i.e. obtain coming from the tobacco additive agent of fresh tobacco leaves.
6th step, is stored in 4 DEG C of refrigerators by the additive subpackage prepared, sealing.
The too low additive of temperature can freeze, multigelation broken meeting additive quality, prevents endogenous enzymes degeneration 4 DEG C of preservations,
The additive water content prepared is about 57%, and due to Nicotiana tabacum L. mesophyll cell and starch nutrition material abundance, moisture is sufficient, 4 DEG C
Temperature can prevent temperature too high and produce and go mouldy.
Because additive of the present invention uses leaf tobacco extract and is prepared from, containing a large amount of nutritional substance poles in Nicotiana tabacum L.
Easily microbiological contamination, additive effect of the present invention is obvious, and usage amount is little every time, and therefore subpackage preserves and prevents from repeating microbiological contamination of uncapping.
The additive effect of the material mixed preparing of 1:1 in mass ratio of product stratum granulosum material of the present invention and mesophyll cell layer
Most preferably, perfume quantity substantially rises, and taste is mellow, and texture is obviously improved, and presents the fragrance such as sweet burnt odor that discharge of the coke, and concentration is the most substantial,
Zest is decreased obviously, and the spurt of nose larynx is basic to disappear, and strength is relatively mild, and miscellaneous QI amount significantly alleviates, and has covered, and pleasant impression is bright
Aobvious improvement；During starch proportion height, presenting flue gas concentration and risen, zest strengthens, strength foot, brings certain simultaneously
Withered and burnt smell and the smell of soil, oral cavity has a certain amount of dry sensation and granular sensation；During mesophyll cell proportion height, present perfume (or spice)
Tolerance substantially rises, and fragrance is plentiful, exquisite in texture, and miscellaneous QI amount significantly alleviates, and pleasant impression significantly improves, but shows the end not simultaneously
Foot, flue gas concentration is general.
Compared with prior art, it has the beneficial effect that the present invention
The present invention provides a kind of tobacco additive agent coming from fresh tobacco leaves, and raw material sources are extensive, with low cost.Additive of the present invention
Containing a large amount of mesophyll cell containing substantial amounts of starch, mesophyll cell is internal containing a large amount of endogenous enzymes, and starch is inside Nicotiana tabacum L. simultaneously
It is hydrolyzed into the saccharide such as glucose, maltose under diastatic action, can generate with tobacco internal aminoacid generation Maillard reaction
Fragrance matter maillard reaction product, makes Medicated cigarette fragrance matter content increase.
After Nicotiana tabacum L. harvesting baking, alcoholization is the important means improving cigarette quality generally used at present, can remove cigarette
The miscellaneous taste of green grass or young crops of leaf and improve the qualities such as flavor and taste, an important mechanisms of tobacco mellowing is the mechanism of enzyme.In flue-cured tobacco
Endogenous enzymes kind and content the most fewer, it is impossible to making Nicotiana tabacum L. sufficiently be refined at short notice, the present invention solves this
One difficult problem, this is owing to fresh tobacco leaves contains a large amount of endogenous enzymes, uniformly can spray by extracting the endogenous enzymes in fresh tobacco leaves
To promote the alcoholization process of flue-cured tobacco on flue-cured tobacco, quality of tobacco can be improved, there is good industrial applications prospect.
Additive of the present invention uses fresh tobacco leaves extract to make, containing a certain amount of Nicotiana tabacum L. endogenous enzymes and substantial amounts of shallow lake
Powder, alcoholized tobacco is had great importance by endogenous enzymes, and Starch Hydrolysis becomes the amino after saccharide with tobacco internal proteolysis
There is Maillard reaction in acid, generates substantial amounts of maillard reaction product.This measure, can not only accelerate the alcoholization of Nicotiana tabacum L., also increase
Add maillard reaction product, increase aroma substance, but owing to starch is different from mesophyll cell mass ratio, the effect that flue-cured tobacco is produced
Fruit has the additive best results of certain difference, starch and the mesophyll cell mixed configuration of 1:1 in mass ratio, and perfume quantity is obvious
Rising, taste is mellow, and texture is obviously improved, and presents the fragrance such as sweet burnt odor that discharge of the coke, and concentration is the most substantial, under zest has substantially
Fall, the spurt of nose larynx is basic to disappear, and strength is relatively mild, and miscellaneous QI amount significantly alleviates, and has covered, and pleasant impression is obviously improved；Starch institute
During accounting example height, presenting flue gas concentration and risen, zest strengthens, strength foot, brings certain withered and burnt smell and soil simultaneously
Stink smell, oral cavity has a certain amount of dry sensation and granular sensation；During mesophyll cell proportion height, present perfume quantity substantially on
Rising, fragrance is plentiful, exquisite in texture, and miscellaneous QI amount significantly alleviates, and pleasant impression significantly improves, but to show the end not enough simultaneously, and flue gas is dense
Degree is general.
Product safety of the present invention, preparation method is simple, so that accomplish not cut while Medicated cigarette reduces tar content
Weak fragrance, it is easy to popularization and application.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and should not be regarded as limiting this
Bright scope.Unreceipted concrete technology or condition person in embodiment, according to the technology described by the document in this area or condition
Or carry out according to product description.Agents useful for same or instrument unreceipted production firm person, be and can be obtained by purchase
Conventional products.
Embodiment 1
A kind of preparation method of the tobacco additive agent coming from fresh tobacco leaves, including as follows:
Nicotiana tabacum L. in the middle part of harvesting ripe, will pump Nicotiana tabacum L. master pulse, then will pump Nicotiana tabacum L. and the temperature of master pulse after tobacco leaf surface wash clean
Degree is that the phosphate buffer of 6 DEG C mixes according to mass volume ratio 1:8, obtains mixture；Described phosphate buffer pH value is 7,
Concentration is 0.03M；
Mixture is at 6 DEG C afterwards, carries out being crushed to crushing residua diameter less than 2mm by disintegrating machine；
Mixture after Po Sui filters and discards residue by 5 layers of sterile gauze, and filtrate is filtered dense with 0.22um hollow fiber column
Contracting, is concentrated into the 18% of original volume；
Then concentrate carries out more than natural subsidence 10h at 3 DEG C, to being divided into 3 layers, take intermediate layer material and orlop material according to
Mass ratio is 2:1 mix homogeneously, i.e. obtains coming from the tobacco additive agent of fresh tobacco leaves, will come from the tobacco additive agent of fresh tobacco leaves
After subpackage, seal in 4 DEG C and preserve.
The tobacco additive agent coming from fresh tobacco leaves is added to the tobacco shred of low-coke tar cigarette, according to conventional preparation technology
It is prepared as Medicated cigarette；Addition is the 0.004% of low-coke tar cigarette tobacco shred weight.
Adding method is: be sprayed on equably after the tobacco additive agent distilled water coming from fresh tobacco leaves is diluted 300 times
On low-coke tar cigarette tobacco shred, after the tobacco shred after sprinkling seals room temperature placement 12h, tobacco shred is put into 90 DEG C of drying bakers and dries 20min；
Then the tobacco shred after drying put into temperature be 22 DEG C, relative humidity be 55% climatic chamber in 8h balance moisture in cut tobacco,
After the tobacco shred after balance is prepared as Medicated cigarette according to conventional preparation technology.
Embodiment 2
A kind of preparation method of the tobacco additive agent coming from fresh tobacco leaves, including as follows:
Nicotiana tabacum L. in the middle part of harvesting ripe, will pump Nicotiana tabacum L. master pulse, then will pump Nicotiana tabacum L. and the temperature of master pulse after tobacco leaf surface wash clean
Degree is that the phosphate buffer of 10 DEG C mixes according to mass volume ratio 1:8, obtains mixture；Described phosphate buffer pH value is 7,
Concentration is 0.05M；
Mixture is at 10 DEG C afterwards, carries out being crushed to crushing residua diameter less than 2mm by disintegrating machine；
Mixture after Po Sui filters and discards residue by 5 layers of sterile gauze, and filtrate is filtered dense with 0.22um hollow fiber column
Contracting, is concentrated into the 22% of original volume；
Then concentrate carries out more than natural subsidence 8h at 8 DEG C, to being divided into 3 layers, take intermediate layer material and orlop material according to
Mass ratio is 1:2 mix homogeneously, i.e. obtains coming from the tobacco additive agent of fresh tobacco leaves, will come from the tobacco additive agent of fresh tobacco leaves
After subpackage, seal in 4 DEG C and preserve.
The tobacco additive agent coming from fresh tobacco leaves is added to the tobacco shred of low-coke tar cigarette, according to conventional preparation technology
It is prepared as Medicated cigarette；Addition is the 0.3% of low-coke tar cigarette tobacco shred weight.
Adding method is: be sprayed on equably after the tobacco additive agent distilled water coming from fresh tobacco leaves is diluted 800 times
On low-coke tar cigarette tobacco shred, after the tobacco shred after sprinkling seals room temperature placement 12h, tobacco shred is put into 90 DEG C of drying bakers and dries 20min；
Then the tobacco shred after drying put into temperature be 22 DEG C, relative humidity be 55% climatic chamber in 12h balance moisture in cut tobacco,
Finally the tobacco shred after balance is prepared as Medicated cigarette according to conventional preparation technology.
Embodiment 3
A kind of preparation method of the tobacco additive agent coming from fresh tobacco leaves, including as follows:
Nicotiana tabacum L. in the middle part of harvesting ripe, will pump Nicotiana tabacum L. master pulse, then will pump Nicotiana tabacum L. and the temperature of master pulse after tobacco leaf surface wash clean
Degree is that the phosphate buffer of 4 DEG C mixes according to mass volume ratio 1:12, obtains mixture；Described phosphate buffer pH value is 7,
Concentration is 0.01M；
Mixture is at 4 DEG C afterwards, carries out being crushed to crushing residua diameter less than 2mm by disintegrating machine；
Mixture after Po Sui filters and discards residue by sterile gauze, filtrate by 0.22um hollow fiber column filtering and concentrating,
It is concentrated into the 20% of original volume；
Then concentrate carries out more than natural subsidence 5h at 4 DEG C, carries out natural subsidence to being divided into 3 layers, takes intermediate layer material and
Lower floor's material is 1:1 mix homogeneously according to mass ratio, i.e. obtains coming from the tobacco additive agent of fresh tobacco leaves.
The tobacco additive agent coming from fresh tobacco leaves is added to the tobacco shred of low-coke tar cigarette, according to conventional preparation technology
It is prepared as Medicated cigarette；Addition is the 0.05% of low-coke tar cigarette tobacco shred weight.
Adding method is: be sprayed on equably after the tobacco additive agent distilled water coming from fresh tobacco leaves is diluted 400 times
On low-coke tar cigarette tobacco shred, after the tobacco shred after sprinkling seals room temperature placement 12h, tobacco shred is put into 90 DEG C of drying bakers and dries 20min；
Then the tobacco shred after drying put into temperature be 22 DEG C, relative humidity be 55% climatic chamber in 10h balance moisture in cut tobacco,
Finally the tobacco shred after balance is prepared as Medicated cigarette according to conventional preparation technology.
Application example and contrast
As a example by Yunnan tobacco K326, plucking the Nicotiana tabacum L. 1000g of fresh mature, clean the impurity of tobacco leaf surface, adding pH value is
7, concentration is 0.1mol phosphate buffer 12L, carries out broken leaf breaking by disintegrating machine, and breaking temperature is maintained at 4-6 DEG C, after crushing
Mixture is extruded by aseptic 5 layers of gauze, carries out solid-liquid separation, obtains filtering residue 691g, Green Water extract 12.4L, pass through
0.22um hollow cellulose post filtering and concentrating, to the 20% of original volume, concentrate more than 4 DEG C of natural subsidence 5h, middle level main
Composition is mesophyll cell and cell debris 42.44g for presenting green, and lower floor is mainly composed of white starch 42.47g, in inciting somebody to action
Interbed mesophyll cell layer material and orlop stratum granulosum material are 2:1,1.5:1,1:1,1:1.5,1:2 mixed preparing in mass ratio
Become tobacco additive agent.
The additive of mentioned component different proportion adds with the addition accounting for tobacco shred weight 0.1%, and additive distilled water is dilute
Being sprayed on tobacco shred after releasing 400 times equably, the tobacco shred after processing is placed individually into plastics valve bag, and after sealing, room temperature places 12h
After, tobacco shred is put into 90 DEG C of drying baker 20min drying cigarette samples and makes endogenous enzymes inactivate.Then it is put into 22 DEG C, relative humidity 55%
Climatic chamber in balance moisture in cut tobacco, reduce the sense organ error caused when smokeing panel test because of moisture difference.With total score for 100
Point, there are ten to have the Medicated cigarette judging panel of qualification smoking result of giving a mark of smokeing panel test and are shown in Table 1:
The tobacco sensory quality smoking result that table 1 enzyme liquid processes
Note: in table 1, starch refers to stratum granulosum material；Mesophyll cell refers to mesophyll cell layer material.
The additive of mentioned component different proportion adds with the addition accounting for tobacco shred weight 0.1%, and additive distilled water is dilute
It is sprayed on equably after releasing 400 times on low-coke tar cigarette tobacco shred, starch and the interpolation of the mesophyll cell mixed configuration of 1:1 in mass ratio
Agent best results, perfume quantity substantially rises, and taste is mellow, and texture is obviously improved, and presents the fragrance such as sweet burnt odor that discharge of the coke, and concentration is very
Enriching, zest is decreased obviously, and the spurt of nose larynx is basic to disappear, and strength is relatively mild, and miscellaneous QI amount significantly alleviates, and has covered,
Pleasant impression is obviously improved；During starch proportion height, presenting flue gas concentration and risen, zest strengthens, strength foot, carries simultaneously
Coming certain withered and burnt smell and the smell of soil, oral cavity has a certain amount of dry sensation and granular sensation；During mesophyll cell proportion height, in
Revealing perfume quantity substantially to rise, fragrance is plentiful, exquisite in texture, and miscellaneous QI amount significantly alleviates, and pleasant impression significantly improves, but shows simultaneously
Strength is not enough, and flue gas concentration is general, has ten to have the Medicated cigarette judging panel of qualification smoking result of giving a mark of smokeing panel test and is shown in Table 2:
The tobacco sensory quality smoking result that table 2 enzyme liquid processes describes
Note: in table 1, starch refers to stratum granulosum material；Mesophyll cell refers to mesophyll cell layer material.
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described.The technology of the industry
Personnel, it should be appreciated that the present invention is not restricted to the described embodiments, simply illustrating this described in above-described embodiment and description
The principle of invention, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, and these become
Change and improvement both falls within scope of the claimed invention.Claimed scope by appending claims and
Equivalent defines.
Claims (10)
1. the preparation method of the tobacco additive agent coming from fresh tobacco leaves, it is characterised in that include as follows:
Nicotiana tabacum L. in the middle part of harvesting ripe, will pump Nicotiana tabacum L. master pulse, then will pump Nicotiana tabacum L. and the temperature of master pulse after tobacco leaf surface wash clean
The phosphate buffer that degree is 4-10 DEG C mixes according to mass volume ratio 1:4-12, obtains mixture；Described phosphate buffer pH
Value is 7, and concentration is 0.01M-0.05M；
Mixture is at 4-10 DEG C afterwards, carries out being crushed to crushing residua diameter less than 2mm by disintegrating machine；
Mixture after Po Sui filters and discards residue by sterile gauze, filtrate by 0.22um hollow fiber column filtering and concentrating,
It is concentrated into the 18-22% of original volume；
Then concentrate carries out natural subsidence to being divided into 3 layers at 3-8 DEG C, takes intermediate layer material and orlop material according to mass ratio
For 2:1 ~ 1:2 mix homogeneously, i.e. obtain coming from the tobacco additive agent of fresh tobacco leaves.
The preparation method of the tobacco additive agent coming from fresh tobacco leaves the most according to claim 1, it is characterised in that include by
Following steps:
Nicotiana tabacum L. in the middle part of harvesting ripe, will pump Nicotiana tabacum L. master pulse, then will pump Nicotiana tabacum L. and the temperature of master pulse after tobacco leaf surface wash clean
Degree is that the phosphate buffer of 4 DEG C mixes according to mass volume ratio 1:12, obtains mixture；Described phosphate buffer pH value is 7,
Concentration is 0.01M；
Mixture is at 4 DEG C afterwards, carries out being crushed to crushing residua diameter less than 2mm by disintegrating machine；
Mixture after Po Sui filters and discards residue by sterile gauze, filtrate by 0.22um hollow fiber column filtering and concentrating,
It is concentrated into the 20% of original volume；
Then concentrate carries out natural subsidence at 4 DEG C, carries out natural subsidence to being divided into 3 layers, takes intermediate layer material and orlop thing
Matter is 1:1 mix homogeneously according to mass ratio, i.e. obtains coming from the tobacco additive agent of fresh tobacco leaves.
The preparation method of the tobacco additive agent coming from fresh tobacco leaves the most according to claim 1, it is characterised in that described
When using sterile gauze to filter, sterile gauze is of five storeys.
The preparation method of the tobacco additive agent coming from fresh tobacco leaves the most according to claim 1, it is characterised in that described
Static balance time is more than 5h；Taking intermediate layer material and orlop material according to mass ratio is 1:1 mix homogeneously, i.e. obtains source
Tobacco additive agent in fresh tobacco leaves.
The preparation method of the tobacco additive agent coming from fresh tobacco leaves the most according to claim 1, it is characterised in that will come from
After the tobacco additive agent subpackage of fresh tobacco leaves, seal in 4 DEG C and preserve.
6. what the preparation method of the tobacco additive agent coming from fresh tobacco leaves described in claim 1-5 prepared comes from fresh tobacco leaves
Tobacco additive agent.
7. what the preparation method of the tobacco additive agent coming from fresh tobacco leaves described in claim 1-5 prepared comes from fresh tobacco leaves
Tobacco additive agent application in preparing Medicated cigarette, it is characterised in that the tobacco additive agent coming from fresh tobacco leaves is added to low Jiao
In the tobacco shred of oil Medicated cigarette, it is prepared as Medicated cigarette according to conventional preparation technology；
Addition is the 0.004%-0.3% of low-coke tar cigarette tobacco shred weight.
The tobacco additive agent coming from fresh tobacco leaves the most according to claim 7 application in preparing Medicated cigarette, its feature exists
In, come from the 0.004%-0.1% that addition is low-coke tar cigarette tobacco shred weight of the tobacco additive agent of fresh tobacco leaves.
The tobacco additive agent coming from fresh tobacco leaves the most according to claim 7 application in preparing Medicated cigarette, its feature exists
In, it is sprayed on low-coke tar cigarette cigarette equably after the tobacco additive agent distilled water coming from fresh tobacco leaves is diluted 300-800 times
On silk.
The tobacco additive agent coming from fresh tobacco leaves the most according to claim 7 application in preparing Medicated cigarette, its feature exists
In, it is sprayed on equably on low-coke tar cigarette tobacco shred after the tobacco additive agent distilled water coming from fresh tobacco leaves is diluted 400 times,
After tobacco shred after sprinkling seals room temperature placement 12h, tobacco shred is put into 90 DEG C of drying bakers and dries 20min；Then the cigarette after drying
Silk put into temperature be 22 DEG C, relative humidity be 55% climatic chamber in 8-12h balance moisture in cut tobacco, finally will be after balance
Tobacco shred is prepared as Medicated cigarette according to conventional preparation technology.
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Cited By (1)
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| CN113017140A (en) * | 2021-03-12 | 2021-06-25 | 云南瑞升烟草技术（集团）有限公司 | Method for preparing endogenous total enzyme by using fresh tobacco and application of endogenous total enzyme |
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