CN106666810B - One grows tobacco grape wine novel tobacco product and preparation method thereof - Google Patents
One grows tobacco grape wine novel tobacco product and preparation method thereof Download PDFInfo
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Abstract
本发明提供一种烟草葡萄酒新型烟草制品,以烟草、葡萄、糖等原料,经活性干酵母、β‑葡萄糖苷酶、纤维素酶混合发酵而成烟草葡萄发酵酒,另一部分采用食用酒精将烟草、葡萄中的可溶性成分进行索氏提取后,两部分复配,并放入橡木片浸泡、陈酿,制成烟草葡萄酒新型烟草制品。本发明可保留烟草、葡萄的功能性成分,具有烟草特征香气,葡萄酒醇厚柔和,酸甜口感,香气饱满丰富,中和烟碱强烈气息,不刺喉辣喉,克服烟粉直接使用的刺激性过大、苦涩味浓重、辛辣味强烈的缺陷,能享受无烟雾形式烟草所带来的满足和快感;色泽为清亮紫红色,具有丰富的营养成分,方便直接饮用,绿色环保,还能给带来消费者新奇、美好的感受。The present invention provides a novel tobacco product of tobacco wine, which is fermented with tobacco, grapes, sugar and other raw materials through active dry yeast, β-glucosidase and cellulase to form tobacco grape fermented wine, and the other part uses edible alcohol to ferment tobacco 1. After the soluble components in the grapes are extracted by Soxhlet, the two parts are compounded, soaked in oak chips, and aged to make new tobacco products of tobacco wine. The invention can retain the functional components of tobacco and grapes, has the characteristic aroma of tobacco, the wine is mellow and soft, has a sweet and sour taste, full and rich aroma, neutralizes the strong smell of nicotine, does not irritate the throat, and overcomes the irritation caused by direct use of tobacco powder Excessively large, strong bitterness, strong spicy taste, you can enjoy the satisfaction and pleasure brought by smokeless tobacco; the color is clear purple red, rich in nutrients, easy to drink directly, green and environmentally friendly, and can also bring you Come to consumers to feel novelty and beauty.
Description
技术领域technical field
本发明涉及无烟烟草技术领域,具体涉及一种烟草葡萄酒新型烟草制品及其制备方法。The invention relates to the technical field of smokeless tobacco, in particular to a novel tobacco wine product and a preparation method thereof.
背景技术Background technique
在新型无烟卷烟在使用过程中没有燃烧形成的烟雾,不会产生二手烟危害,正在被越来越多的国内外消费者接受。新型无烟烟草产品有多种形式,包括鼻烟、嚼烟、含烟等,但正常生产嚼烟的周期约为3~4个月,加大了生产成本,且产品的数量规模和质量风格不能根据市场需求和消费者的口味迅速作出调整;由于在消费者口中形成大量的残渣,也可能导致公共卫生方面的问题。目前,国内烟草领域的研究人员应对国际烟草领域发展的新变化,开发适合国内烟草消费者的无烟气烟草制品,如口含烟、嚼烟等,采用烟叶或烟粉放在嘴中品尝,通过吸收汁液中的烟碱和其他成分满足刺激性的需要。现有的口含烟、嚼烟制品由于含水率较高(一般在30份~60份),易于产生霉变,不利于长期保存,同时在咀嚼时由于烟草特有的刺激性和辛辣、苦涩等气息,因而难以被人接受,限制了产品的推广。In the process of using the new smokeless cigarettes, there is no smoke formed by combustion, and there is no second-hand smoke hazard, and it is being accepted by more and more domestic and foreign consumers. There are many forms of new smokeless tobacco products, including snuff, chewing tobacco, and tobacco, etc., but the normal production cycle of chewing tobacco is about 3 to 4 months, which increases the production cost, and the quantity, scale and quality of the products cannot be matched. Quickly adjusted to market needs and consumer tastes; may also cause public health problems due to formation of large amounts of residue in the consumer's mouth. At present, researchers in the domestic tobacco field respond to new changes in the development of the international tobacco field, and develop smokeless tobacco products suitable for domestic tobacco consumers, such as snus, chewing tobacco, etc., using tobacco leaves or powdered tobacco to taste in the mouth, Satisfy the need for stimulation by absorbing nicotine and other components in the juice. Existing snus and chewing tobacco products are prone to mildew due to their high moisture content (generally 30-60 parts), which is not conducive to long-term storage. Breath, thus be difficult to be accepted by the people, have limited the promotion of product.
新型无烟气烟草制品的感官冲击强度主要受烟草原料的感官特性影响,目前国内开发的无烟气烟草制品的其主要特征为通过各类调节剂、保润剂、香味剂等进行配方调制,工艺大多为直接使用烟草原料研磨、调味、装袋,存在刺激性过大、苦涩味浓重、辛辣味强烈的缺陷。The sensory impact strength of new smokeless tobacco products is mainly affected by the sensory properties of tobacco raw materials. The main feature of domestically developed smokeless tobacco products is that they are formulated with various regulators, humectants, flavoring agents, etc. Most of the processes are directly using tobacco raw materials for grinding, seasoning, and bagging, which have the defects of excessive irritation, strong bitter and astringent taste, and strong spicy taste.
葡萄是一种最重要的水果,在全世界所有水果中,其产量和栽培面积一直居首位,被称为“水晶明珠”。葡萄果色艳丽、汁多味美、营养丰富,含糖量达10%~30%,含有丰富的酸和多种维生素外,还含有白藜芦醇、原花青素、单宁、天然色素等功能性营养成分,除了能够帮助人们改善睡眠外,还有很多功效,多量果酸有助于消化,补虚健胃。葡萄汁可能含有睡眠辅助激素褪黑素,是大脑中松果腺分泌的物质,可以帮助调节睡眠周期,并能治疗失眠。葡萄是温补阳气的食品,不仅具有养肝的效果,而且修复气血,达到养颜美容的效果。葡萄所含较多的糖分中,大部分是容易被人体直接吸收的葡萄糖,葡萄中含较多的酒石酸,更有帮助消化的作用。葡萄味甘酸、性平,有滋阴补血、强健筋骨、通利小便的功效,是一种滋补性很强的水果;适用于妊娠贫血、肺虚咳嗽、心悸盗汗、风湿痹痛、水肿等症。还对急性泌尿系统感染有疗效。葡萄皮中的白藜芦醇不仅能抑制发炎物质的运作,并能抑制已恶变细胞扩散,有较强的防癌抗癌功能。葡萄果实中,葡萄糖、有机酸、氨基酸、维生素的含量都很丰富,可补益和兴奋大脑神经,对治疗神经衰弱有一定效果。葡萄皮、肉、籽中有丰富的白藜芦醇,具有降血脂、预防动脉硬化、增强免疫力等作用,特别是紫葡萄皮中的黄酮类物质,还有降低血压的功效,葡萄皮中的花青素,具有强抗氧化、抗突变、减轻肝机能障碍、保护心血管等功能。Grape is one of the most important fruits. Among all fruits in the world, its output and cultivated area have always ranked first, and it is called "Crystal Pearl". Grape fruit is bright in color, juicy and delicious, rich in nutrition, with a sugar content of 10% to 30%, rich in acids and multivitamins, and also contains functional nutrients such as resveratrol, proanthocyanidins, tannins, and natural pigments. The ingredients, in addition to helping people improve their sleep, also have many effects, a large amount of fruit acid helps digestion, nourishes deficiency and strengthens stomach. Grape juice may contain the sleep aid hormone melatonin, a substance secreted by the pineal gland in the brain, which can help regulate sleep cycles and treat insomnia. Grape is a food that warms and invigorates yang, not only has the effect of nourishing the liver, but also restores qi and blood, so as to achieve the effect of beautifying and beautifying. Most of the sugar contained in grapes is glucose, which is easily absorbed by the human body. Grapes contain more tartaric acid, which is more helpful for digestion. Grape tastes sweet and sour, and has a mild nature. It has the effects of nourishing yin and blood, strengthening muscles and bones, and facilitating urination. It is a fruit with strong nourishing properties; it is suitable for anemia in pregnancy, cough due to lung deficiency, palpitations, night sweats, rheumatic arthralgia, edema, etc. . It is also effective for acute urinary system infections. Resveratrol in grape skin can not only inhibit the operation of inflammatory substances, but also inhibit the proliferation of malignant cells, and has strong anti-cancer and anti-cancer functions. Grape fruit is rich in glucose, organic acids, amino acids, and vitamins, which can nourish and excite the brain nerves, and have a certain effect on the treatment of neurasthenia. Grape skin, meat, and seeds are rich in resveratrol, which has the effects of lowering blood fat, preventing arteriosclerosis, and enhancing immunity. Especially the flavonoids in purple grape skin have the effect of lowering blood pressure. Anthocyanins have strong anti-oxidation, anti-mutation, alleviate liver dysfunction, protect cardiovascular and other functions.
红葡萄酒中含有类黄酮化合物,特别是其中的槲皮酮能够降低血小板的黏性,适量饮用红葡萄酒能起到保护心脏、减少心血管疾病危害的作用。Red wine contains flavonoids, especially quercetin, which can reduce the viscosity of platelets. Drinking red wine in moderation can protect the heart and reduce the risk of cardiovascular diseases.
烟草中含有蛋白质,烟碱,挥发酸,谷氨酰胺、天冬酰胺等氨基酸,还原糖、果胶、淀粉、糖酯糖苷等营养物质。在烟草制品中,烟草的香气和吸味是至关重要的。现有技术中,采用自然醇化、生物酶发酵处理的方法提高烟叶的质量,促使烟叶理化特性发生深刻变化,烟叶香气明显改善。Tobacco contains protein, nicotine, volatile acid, glutamine, asparagine and other amino acids, reducing sugar, pectin, starch, sugar ester glycoside and other nutrients. In tobacco products, the aroma and taste of tobacco are crucial. In the prior art, the method of natural alcoholization and biological enzyme fermentation treatment is used to improve the quality of tobacco leaves, promote profound changes in the physical and chemical properties of tobacco leaves, and significantly improve the aroma of tobacco leaves.
本发明将烟草、葡萄、糖等,复合发酵制备烟草葡萄发酵酒,与烟草、葡萄混合提取液复配成为烟草葡萄酒新型烟草制品,既能保留烟草特征风味,又有葡萄酒酸甜适口、清香醇厚口感,增加人体的营养,该产品揉和了烟草、葡萄酒的特点,口感协调、新颖,是一种具有功能活性的液态新型烟草制品,尚未见报道。In the present invention, tobacco, grapes, sugar, etc. are combined and fermented to prepare tobacco and grape fermented wine, and the mixed extract of tobacco and grapes is compounded to form a new type of tobacco wine product, which can not only retain the characteristic flavor of tobacco, but also have sweet and sour taste, mellow fragrance of wine Taste, increase the nutrition of the human body, this product blends the characteristics of tobacco and wine, the taste is harmonious and novel, it is a new type of liquid tobacco product with functional activity, which has not been reported yet.
发明内容Contents of the invention
本发明的目的是提供一种烟草葡萄酒新型烟草制品,以烟草、葡萄、糖等原料,经活性干酵母、β-葡萄糖苷酶、纤维素酶混合发酵而成烟草葡萄发酵酒,另一部分采用食用酒精将烟草、葡萄中的可溶性成分进行索氏提取后,两部分复配,并放入橡木片浸泡、陈酿,制成烟草葡萄酒新型烟草制品,保留烟草、葡萄的功能性成分,具有烟草特征香气,葡萄酒醇厚柔和,酸甜口感,香气饱满丰富,中和烟碱强烈气息,不刺喉辣喉,克服烟粉直接使用的刺激性过大、苦涩味浓重、辛辣味强烈的缺陷,能享受无烟雾形式烟草所带来的满足和快感;色泽为清亮紫红色,具有丰富的营养成分,方便直接饮用,绿色环保,还能给带来消费者新奇、美好的感受。The purpose of the present invention is to provide a novel tobacco product of tobacco wine, which is fermented by active dry yeast, β-glucosidase, and cellulase from raw materials such as tobacco, grapes, and sugar, and the other part is edible wine. Alcohol extracts the soluble components in tobacco and grapes by Soxhlet, then recombines the two parts, soaks them in oak chips, and ages them to make a new type of tobacco wine, which retains the functional components of tobacco and grapes and has the characteristic aroma of tobacco. , the wine is mellow and soft, with a sweet and sour taste, full and rich aroma, neutralizes the strong smell of nicotine, does not irritate the throat, overcomes the defects of excessive irritation, strong bitterness, and strong spicy taste caused by direct use of tobacco powder, and can enjoy unlimited The satisfaction and pleasure brought by tobacco in the form of smoke; the color is clear purple red, rich in nutrients, convenient to drink directly, green and environmentally friendly, and can also bring consumers novel and beautiful feelings.
本发明通过以下技术方案实现:The present invention is realized through the following technical solutions:
一种烟草葡萄酒新型烟草制品,采用优质烟粉、优质紫红葡萄为原料,具体包括如下步骤:A new type of tobacco wine tobacco product, using high-quality tobacco powder and high-quality purple grapes as raw materials, specifically comprising the following steps:
(1)将破碎的葡萄果浆和优质烟粉按照重量比1:5~20放入不锈钢发酵罐中,加入葡萄果浆和烟粉混合物质量0.1~0.3%的复合酶,所述复合酶包括β-葡萄糖苷酶和纤维素酶;在水中,加入占水17.5~35%质量比的活性干酵母、酵母营养素(NH3H2PO4)和糖,活性干酵母、酵母营养素(NH3H2PO4)和糖的质量比为1:1.5:1,不停地搅拌,直到酵母再生,冒起大量泡沫,将酵母液按葡萄果浆和烟粉混合物质量比15~30%加入,搅拌混合均匀,总混合物占发酵罐容积的60~80%;在15~30℃温度条件下,浸渍发酵24h~36h后,加入占水10~12.5%质量比的单宁;检测混合物的糖度,在混合烟草葡萄浆的糖度消耗一半时,加入白砂糖,使烟草葡萄果浆混合物的含糖量达到200-250g/L;控制温度15~28℃进行发酵5~7天,当发酵汁含糖量达到5g/L以下时,进行皮渣分离,分离自流汁,保留;分离的皮渣,进行压榨,压榨汁保留;将自流汁和压榨汁合并得到烟草葡萄发酵酒,备用;(1) Put the crushed grape pulp and high-quality smoke powder into a stainless steel fermenter according to the weight ratio of 1:5-20, add a compound enzyme with a mass of 0.1-0.3% of the mixture of grape pulp and smoke powder, and the compound enzyme includes β-glucosidase and cellulase; in water, add active dry yeast, yeast nutrient (NH 3 H 2 PO 4 ) and sugar, active dry yeast, yeast nutrient (NH 3 H 2 PO 4 ) and sugar at a mass ratio of 1:1.5:1, stirring continuously until the yeast regenerates and produces a lot of foam, then add the yeast liquid at a mass ratio of 15% to 30% of the mixture of grape pulp and tobacco powder, and stir Mix evenly, the total mixture accounts for 60-80% of the volume of the fermenter; at a temperature of 15-30°C, after soaking and fermenting for 24h-36h, add tannins accounting for 10-12.5% by mass of water; detect the sugar content of the mixture, When half of the sugar content of the mixed tobacco grape pulp is consumed, white granulated sugar is added to make the sugar content of the tobacco grape pulp mixture reach 200-250g/L; fermentation is carried out at a temperature of 15-28°C for 5-7 days, when the sugar content of the fermented juice When it reaches below 5g/L, separate the skin dregs, separate the artesian juice, and keep it; separate the skin dregs, squeeze it, and keep the squeezed juice; combine the artesian juice and the squeezed juice to obtain tobacco grape fermented wine, and set aside;
(2)烟草葡萄酒萃取液的制备:将步骤(1)中分离压榨后的皮渣,采用质量比5~10倍食用酒精放在下部,对顶部的分离压榨烟草葡萄皮渣进行45~55℃索氏提取1~3次,每次3~5h,合并提取液,得到烟草葡萄酒萃取液;(2) Preparation of tobacco wine extract: put the skin pomace after separation and pressing in step (1) into the lower part with 5 to 10 times the edible alcohol in mass ratio, and heat the separated and pressed tobacco grape skin pomace at 45 to 55 ° C on the top. Soxhlet extraction 1 to 3 times, 3 to 5 hours each time, combined extracts to obtain tobacco wine extracts;
(3)调配:将上述步骤(1)的烟草葡萄发酵酒和步骤(2)的烟草葡萄酒萃取液以一定比例混合,使所得的烟草葡萄酒酒精度达到8~12%(v/v);(3) Blending: mix the tobacco grape fermented wine of the above step (1) with the tobacco wine extract of the step (2) in a certain ratio, so that the alcohol content of the tobacco wine of the gained reaches 8-12% (v/v);
(4)陈酿:向步骤(3)的烟草葡萄调配酒中加入SO2,保持游离SO225~200mg/L,并将橡木片按烟草葡萄酒质量的0.2~0.4%浸泡在烟草葡萄酒中,陈酿3~12个月,定期转罐去除沉淀并更换橡木片;(4) Aging: add SO 2 to the tobacco grape blended wine in step (3), keep the free SO 2 25-200mg/L, soak the oak chips in the tobacco wine by 0.2-0.4% of the mass of the tobacco wine, and age 3 to 12 months, regularly transfer tanks to remove sediment and replace oak chips;
(5)除菌过滤澄清化:采用模式过滤机,过滤孔径小于0.4微米,过滤除掉细菌、酵母细胞和其他杂质,使烟草葡萄酒澄清透亮,香气口感更纯粹;(5) Sterilization and filtration clarification: use a model filter with a filter aperture of less than 0.4 microns to filter out bacteria, yeast cells and other impurities, so that the tobacco wine is clear and translucent, and the aroma and taste are purer;
(6)热处理提高稳定性和促氧化:将发酵罐中的烟草葡萄酒加热到60~70℃,保温12~18min,趁热装瓶,自然冷却,达到提高烟草葡萄酒稳定性作用,因为加热有杀菌作用,防止微生物引起的混浊沉淀,破坏烟草葡萄酒中多酚氧化酶,防止烟草葡萄酒的氧化混浊;(6) Heat treatment to improve stability and promote oxidation: heat the tobacco wine in the fermenter to 60-70°C, keep it warm for 12-18 minutes, bottle it while it is hot, and cool it naturally to improve the stability of tobacco wine, because heating can sterilize function, prevent turbid precipitation caused by microorganisms, destroy polyphenol oxidase in tobacco wine, and prevent oxidative turbidity of tobacco wine;
(7)成品:灌装。(7) Finished product: filling.
进一步的,葡萄果浆的制备方法为:选用果皮紫红色的葡萄,剔除病烂、病虫、生青果,用清水洗去表面污物,用滚筒式或离心式破碎将果实压破,再经除梗机去掉果梗,即可得到所述葡萄果浆。Further, the preparation method of grape pulp is as follows: select grapes with purple-red peels, remove diseased, pests, and green fruits, wash away surface dirt with water, crush the fruits with drum or centrifugal crushing, and then The destemmer removes the fruit stems to obtain the grape pulp.
进一步的,上述步骤(1)、(2)中的水为蒸馏水或纯净水。Further, the water in the above steps (1), (2) is distilled water or pure water.
进一步的,上述步骤(1)中的纤维素酶为食品级,酶活为10万~20万u/g;β-葡萄糖苷酶为食品级,酶活为100~10000u/g;纤维素酶和β-葡萄糖苷酶质量比为0.5~1:1。Further, the cellulase in the above step (1) is food grade with an enzyme activity of 100,000 to 200,000 u/g; the β-glucosidase is food grade with an enzyme activity of 100 to 10,000 u/g; the cellulase The mass ratio to β-glucosidase is 0.5-1:1.
进一步的,所述的烟粉为云南、津巴布韦等地优质烟叶的超微粉末。Further, the tobacco powder is ultrafine powder of high-quality tobacco leaves from Yunnan, Zimbabwe and other places.
本发明的有益效果是:The beneficial effects of the present invention are:
本发明以烟草、葡萄为原料,经β-葡萄糖苷酶、纤维素酶复合发酵生香,再经活性干酵母发酵而成烟草葡萄酒,其中纤维素酶、β-葡萄糖苷酶协同作用,降解转化烟草、葡萄中的细胞壁大分子物质,并转化成香气小分子物质或香味前体物,还促进酒精发酵出汁,提高香气品质和口感、促进发酵,活性干酵母酒精发酵保证发酵的纯正性,改善烟草葡萄酒的风味,发酵一段时间后,还补充适量蔗糖,促进酒精发酵,并加入适量单宁,有效保护葡萄中的花青素等天然色素成分,使色素更稳定,并能改善烟草葡萄酒的口感,增加结构感;所压榨的烟草葡萄渣,再经食用酒精将其中的有效成分多次索氏提取,两者按一定比例复配后,加入SO2具有杀灭杂菌和野生酵母的作用,然后放入橡木片浸渍陈化发酵,橡木的芳香成分和单宁物质浸溶到烟草葡萄酒中,比橡木桶成本低,多片橡木片比橡木桶与烟草葡萄酒接触面积更大,且橡木片使用方便,生产成本低,提高产品质量效果好;采用模式过滤机,过滤孔径小于0.4微米,过滤除掉细菌、酵母细胞和其他杂质,使烟草葡萄酒澄清透亮,香气口感更纯粹;热处理提高稳定性和促氧化:将发酵罐中的烟草葡萄酒加热到60~70℃,保温12~18min,趁热装瓶,自然冷却,达到提高烟草葡萄酒稳定性作用,因为加热有杀菌作用,防止微生物引起的混浊沉淀,破坏烟草葡萄酒中多酚氧化酶,防止烟草葡萄酒的氧化混浊。最后制成的烟草葡萄酒新型烟草制品,保留烟草、葡萄的功能性成分,含有多种类黄酮类物质、花青素,具有强抗氧化、抗突变、减轻肝机能障碍、保护心血管、降低血压等功能,营养丰富。烟草葡萄酒新型烟草制品具有烟草特征香气和葡萄酒酸甜醇和轻柔口感,香气饱满丰富,不刺喉辣喉,克服烟粉直接使用的刺激性过大、苦涩味浓重、辛辣味强烈的缺陷,能享受无烟雾形式烟草和一定的酒精含量所带来的满足和兴奋愉悦感;色泽为清亮紫红色,装瓶方便携带,能直接饮用,绿色环保,带给消费者新奇、美好的感受。The present invention uses tobacco and grapes as raw materials, produces aroma through compound fermentation of β-glucosidase and cellulase, and then ferments with active dry yeast to form tobacco wine, in which cellulase and β-glucosidase act synergistically to degrade and transform The cell wall macromolecules in tobacco and grapes are transformed into aroma small molecules or aroma precursors, and also promote alcohol fermentation to produce juice, improve aroma quality and taste, and promote fermentation. Alcoholic fermentation with active dry yeast ensures the purity of fermentation and improves For the flavor of tobacco wine, after a period of fermentation, add an appropriate amount of sucrose to promote alcohol fermentation, and add appropriate amount of tannins to effectively protect the natural pigment components such as anthocyanins in grapes, make the pigment more stable, and improve the taste of tobacco wine , to increase the sense of structure; the squeezed tobacco grape marc, and then the active ingredients of which are extracted by Soxhlet for many times with edible alcohol, after the two are compounded according to a certain ratio, adding SO 2 has the effect of killing miscellaneous bacteria and wild yeast, Then put in oak chips for immersion aging and fermentation, the aroma components and tannins of oak are soaked into tobacco wine, which is cheaper than oak barrels, and the contact area between multiple oak chips and tobacco wine is larger than that of oak barrels, and oak chips are used Convenience, low production cost, and good effect in improving product quality; using a pattern filter, the filter pore size is less than 0.4 microns, filtering out bacteria, yeast cells and other impurities, making tobacco wine clear and clear, and the aroma and taste are more pure; heat treatment improves stability and Pro-oxidation: heat the tobacco wine in the fermenter to 60-70°C, keep it warm for 12-18 minutes, bottle it while it is hot, and cool it naturally to improve the stability of the tobacco wine, because heating has a bactericidal effect and prevents turbidity and precipitation caused by microorganisms , Destroy polyphenol oxidase in tobacco wine, prevent oxidation turbidity of tobacco wine. The final tobacco and wine new-type tobacco products retain the functional components of tobacco and grapes, and contain a variety of flavonoids and anthocyanins, which have strong anti-oxidation, anti-mutation, alleviate liver dysfunction, protect cardiovascular, and lower blood pressure. Functional and nutritious. Tobacco and wine new-type tobacco products have the characteristic aroma of tobacco and the sweet and sour taste of wine and soft taste. Satisfaction and excitement brought by smokeless form of tobacco and a certain alcohol content; the color is clear purple red, easy to carry in bottles, can be drunk directly, green and environmentally friendly, and brings novelty and good feelings to consumers.
本发明工艺条件简单,可直接使用现有生产设备,无需改造,所用的原料天然环保无害等优点,具有较大的工业应用前景。The invention has the advantages of simple process conditions, the existing production equipment can be directly used without modification, the raw materials used are natural, environmentally friendly and harmless, and has great industrial application prospects.
具体实施方式detailed description
以下结合具体实施例对本发明进行详细地说明。下面实施中各组分均按照重量份进行配比。The present invention will be described in detail below in conjunction with specific embodiments. In the following implementation, each component is proportioned according to parts by weight.
实施例1Example 1
一种烟草葡萄酒新型烟草制品,采用优质云南烟粉、优质紫红葡萄为原料,具体包括如下步骤:A novel tobacco wine product made of tobacco and wine, which uses high-quality Yunnan tobacco powder and high-quality purple grapes as raw materials, and specifically includes the following steps:
(1)烟草葡萄发酵酒的制备:选用果皮紫红色的葡萄,剔除病烂、病虫、生青果,用清水洗去表面污物。用滚筒式或离心式破碎将果实压破,再经除梗机去掉果梗,将破碎的葡萄果浆和优质烟粉(重量比1:5)放入不锈钢发酵罐中,加入葡萄果浆和烟粉混合物质量0.1%的复合酶(β-葡萄糖苷酶,酶活10000u/g,纤维素酶,酶活20万u/g,均为食品级);在纯净水中,加入占水18%质量比的活性干酵母、酵母营养素(NH3H2PO4)和麦芽糖(三者质量比1:1.5:1),不停地搅拌,直到酵母再生,冒起大量泡沫,再将酵母液按葡萄果浆和烟粉混合物质量的15%加入,搅拌混合均匀,总混合物占发酵罐容积的70%;在16℃条件下,浸渍发酵24h后,加入占水10%质量比的单宁;检测混合物的糖度,在混合烟草葡萄浆的糖度消耗一半时,加入白砂糖,使烟草葡萄果浆混合物的含糖量达到200g/L;控制温度16℃进行发酵5天,当发酵汁含糖量达到5g/L以下时,进行皮渣分离,分离自流汁,保留;分离的皮渣,进行压榨,压榨汁保留;将自流汁和压榨汁合并,备用,称为烟草葡萄发酵酒;(1) Preparation of tobacco grape fermented wine: select grapes with purplish red skin, remove diseased and rotten fruits, diseases and insect pests, and raw green fruits, and wash away surface dirt with clear water. The fruit is crushed by drum type or centrifugal crushing, and then the fruit stem is removed by a destemmer, the crushed grape pulp and high-quality smoke powder (weight ratio 1:5) are put into a stainless steel fermentation tank, and the grape pulp and 0.1% compound enzyme (beta-glucosidase, enzyme activity 10000u/g, cellulase, enzyme activity 200000u/g, both food grade) of tobacco powder mixture mass; in purified water, add 18% water Ratio of active dry yeast, yeast nutrient (NH 3 H 2 PO 4 ) and maltose (the mass ratio of the three is 1:1.5:1), stirring continuously until the yeast regenerates and a lot of foam occurs, and then press the yeast liquid into grapes Add 15% of the mass of the mixture of fruit pulp and tobacco powder, stir and mix evenly, the total mixture accounts for 70% of the volume of the fermentation tank; at 16°C, after soaking and fermenting for 24 hours, add tannins accounting for 10% by mass of water; test the mixture When the sugar content of the mixed tobacco grape pulp is half consumed, white granulated sugar is added to make the sugar content of the tobacco grape pulp mixture reach 200g/L; the fermentation is carried out at a temperature of 16°C for 5 days, when the sugar content of the fermented juice reaches 5g When the /L is below, separate the skin dregs, separate the artesian juice, and keep it; separate the skin dregs, squeeze it, and keep the squeezed juice; combine the artesian juice and the squeezed juice, and reserve it, which is called tobacco grape fermented wine;
(2)烟草葡萄酒萃取液的制备:将步骤1)中分离压榨后的皮渣,采用质量比5倍的食用酒精放在下部,对顶部的分离压榨烟草葡萄皮渣进行55℃索氏提取1次,5h,得到烟草葡萄酒萃取液;(2) Preparation of tobacco wine extract: put the pomace separated and squeezed in step 1) into the lower part with edible alcohol with a mass ratio of 5 times, and perform Soxhlet extraction at 55°C on the top separated and pressed tobacco grape pomace Second, 5h, obtain tobacco wine extract;
(3)调配:将上述步骤1)的烟草葡萄发酵酒和步骤2)的烟草葡萄酒萃取液以一定比例混合,使所得的烟草葡萄酒酒精度达到8%(v/v);(3) Blending: mix the tobacco grape fermented wine in the above step 1) with the tobacco wine extract in step 2) in a certain proportion, so that the alcohol content of the tobacco wine obtained can reach 8% (v/v);
(4)陈酿:向步骤3)的烟草葡萄调配酒中加入SO2,保持游离SO225mg/L,并将橡木片按烟草葡萄酒质量的0.2%浸泡在烟草葡萄酒中,陈酿3个月,定期转罐去除沉淀并更换橡木片;(4) aging: add SO 2 to the tobacco grape blended wine in step 3), keep free SO 2 25mg/L, and soak the oak chips in the tobacco wine by 0.2% of the quality of the tobacco wine, age for 3 months, and regularly Transfer tanks to remove sediment and replace oak chips;
(5)除菌过滤澄清化:采用模式过滤机,过滤孔径小于0.4微米,过滤除掉细菌、酵母细胞和其他杂质,使烟草葡萄酒澄清透亮,香气口感更纯粹;(5) Sterilization and filtration clarification: use a model filter with a filter aperture of less than 0.4 microns to filter out bacteria, yeast cells and other impurities, so that the tobacco wine is clear and translucent, and the aroma and taste are purer;
(6)热处理提高稳定性和促氧化:将发酵罐中的烟草葡萄酒加热到60℃,保温18min,趁热装瓶,自然冷却,达到提高烟草葡萄酒稳定性作用,因为加热有杀菌作用,防止微生物引起的混浊沉淀,破坏烟草葡萄酒中多酚氧化酶,防止烟草葡萄酒的氧化混浊。(6) Heat treatment to improve stability and promote oxidation: heat the tobacco wine in the fermenter to 60 ° C, keep it warm for 18 minutes, bottle it while it is hot, and cool it naturally to improve the stability of tobacco wine, because heating has a bactericidal effect and prevents microorganisms The turbid precipitation caused destroys the polyphenol oxidase in tobacco wine and prevents the oxidative turbidity of tobacco wine.
(7)成品:灌装。(7) Finished product: filling.
烟草葡萄酒新型烟草制品具有烟草强烈浓郁香气和葡萄酒酸甜醇和口感,香气丰富,克服烟粉直接使用的刺激性过大、苦涩味浓重、辛辣味强烈的缺陷,能享受无烟雾形式烟草和一定的酒精含量所带来的满足和兴奋愉悦感;色泽为清亮紫棕色,装瓶方便携带,能直接饮用,绿色环保,带给消费者新奇、美好的感受。Tobacco and wine new tobacco products have a strong and rich aroma of tobacco and sweet and sour taste of wine. The aroma is rich, and it overcomes the defects of excessive irritation, strong bitterness and strong spicy taste when used directly with tobacco powder, and can enjoy smoke-free form of tobacco and a certain Satisfaction and excitement brought by the alcohol content; the color is clear purple-brown, the bottle is easy to carry, can be drunk directly, green and environmentally friendly, and brings novelty and good feelings to consumers.
实施例2Example 2
一种烟草葡萄酒新型烟草制品,采用优质烟粉、优质紫红葡萄为原料,具体包括如下步骤:A new type of tobacco wine tobacco product, using high-quality tobacco powder and high-quality purple grapes as raw materials, specifically comprising the following steps:
(1)烟草葡萄发酵酒的制备:选用果皮紫红色的葡萄,剔除病烂、病虫、生青果,用清水洗去表面污物。用滚筒式或离心式破碎将果实压破,再经除梗机去掉果梗,将破碎的葡萄果浆和优质烟粉(重量比1:10)放入不锈钢发酵罐中,加入葡萄果浆和烟粉混合物质量0.2%的复合酶(β-葡萄糖苷酶,酶活5000u/g,纤维素酶,酶活15万u/g,均为食品级);在蒸馏水中,加入水24%质量的活性干酵母、酵母营养素(NH3H2PO4)和糖(质量比1:1.5:1),不停地搅拌,直到酵母再生,冒起大量泡沫,将酵母液按葡萄果浆和烟粉混合物质量的21%加入,搅拌混合均匀,总混合物占发酵罐容积的75%;在20℃温度条件下,浸渍发酵30h后,加入水11%质量的单宁;检测混合物的糖度,在混合烟草葡萄浆的糖度消耗一半时,加入白砂糖,使烟草葡萄果浆混合物的含糖量达到225g/L;控制温度23℃进行发酵6天,当发酵汁含糖量达到5g/L以下时,进行皮渣分离,分离自流汁,保留;分离的皮渣,进行压榨,压榨汁保留;将自流汁和压榨汁合并,备用,称为烟草葡萄发酵酒;(1) Preparation of tobacco grape fermented wine: select grapes with purplish red skin, remove diseased and rotten fruits, diseases and insect pests, and raw green fruits, and wash away surface dirt with clear water. Use drum or centrifugal crushing to crush the fruit, then remove the stems through a destemmer, put the crushed grape pulp and high-quality smoke powder (weight ratio 1:10) into a stainless steel fermentation tank, add grape pulp and 0.2% compound enzyme (beta-glucosidase, enzyme activity 5000u/g, cellulase, enzyme activity 150,000u/g, both food grade) of tobacco powder mixture mass; in distilled water, add water 24% mass Active dry yeast, yeast nutrient (NH 3 H 2 PO 4 ) and sugar (mass ratio 1:1.5:1), keep stirring until the yeast regenerates and produces a lot of foam, press the yeast liquid into grape pulp and smoke powder Add 21% of the mass of the mixture, stir and mix evenly, and the total mixture accounts for 75% of the volume of the fermenter; at 20°C, after soaking and fermenting for 30 hours, add tannins with 11% of the mass of water; detect the sugar content of the mixture, and in the blended tobacco When half of the sugar content of the grape pulp is consumed, add white sugar to make the sugar content of the tobacco grape pulp mixture reach 225g/L; control the temperature at 23°C to ferment for 6 days, and when the sugar content of the fermented juice reaches below 5g/L, carry out Separation of skin dregs, separation of artesian juice, and retention; separated skin dregs, pressing, and retention of squeezed juice; combining artesian juice and squeezed juice for later use, called tobacco grape fermented wine;
(2)烟草葡萄酒萃取液的制备:将步骤1)中分离压榨后的皮渣,采用质量比8倍食用酒精放在下部,对顶部的分离压榨烟草葡萄皮渣进行50℃索氏提取2次,每次4h,合并提取液,得到烟草葡萄酒萃取液;(2) Preparation of tobacco wine extract: put the pomace separated and squeezed in step 1) into the lower part with edible alcohol with a mass ratio of 8 times, and perform Soxhlet extraction twice at 50°C on the top separated and pressed tobacco grape pomace , 4 hours each time, combining the extracts to obtain tobacco wine extracts;
(3)调配:将上述步骤1)的烟草葡萄发酵酒和步骤2)的烟草葡萄酒萃取液以一定比例混合,使所得的烟草葡萄酒酒精度达到10%(v/v);(3) Blending: mix the tobacco grape fermented wine in the above step 1) with the tobacco wine extract in step 2) in a certain proportion, so that the alcohol content of the tobacco wine obtained can reach 10% (v/v);
(4)陈酿:向步骤3)的烟草葡萄调配酒中加入SO2,保持游离SO250mg/L,并将橡木片按烟草葡萄酒质量的0.3%浸泡在烟草葡萄酒中,陈酿5个月,定期转罐去除沉淀并更换橡木片。(4) aging: add SO 2 to the tobacco grape blended wine in step 3), keep free SO 2 50mg/L, and soak the oak chips in the tobacco wine by 0.3% of the quality of the tobacco wine, age for 5 months, and regularly Transfer tanks to remove sediment and replace oak chips.
(5)除菌过滤澄清化:采用模式过滤机,过滤孔径小于0.4微米,过滤除掉细菌、酵母细胞和其他杂质,使烟草葡萄酒澄清透亮,香气口感更纯粹;(5) Sterilization and filtration clarification: use a model filter with a filter aperture of less than 0.4 microns to filter out bacteria, yeast cells and other impurities, so that the tobacco wine is clear and translucent, and the aroma and taste are purer;
(6)热处理提高稳定性和促氧化:将发酵罐中的烟草葡萄酒加热到65℃,保温15min,趁热装瓶,自然冷却,达到提高烟草葡萄酒稳定性作用,因为加热有杀菌作用,防止微生物引起的混浊沉淀,破坏烟草葡萄酒中多酚氧化酶,防止烟草葡萄酒的氧化混浊。(6) Heat treatment to improve stability and promote oxidation: heat the tobacco wine in the fermenter to 65 ° C, keep it warm for 15 minutes, bottle it while it is hot, and cool it naturally to improve the stability of tobacco wine, because heating has a bactericidal effect and prevents microorganisms The turbid precipitation caused destroys the polyphenol oxidase in tobacco wine and prevents the oxidative turbidity of tobacco wine.
(7)成品:灌装。(7) Finished product: filling.
烟草葡萄酒新型烟草制品具有烟草明显香气和葡萄酒酸甜轻柔口感,香气较丰富,口感协调,不刺喉辣喉,克服烟粉直接使用的刺激性过大、苦涩味浓重、辛辣味强烈的缺陷,能享受无烟雾形式烟草和一定的酒精含量所带来的满足和兴奋愉悦感;色泽为清亮紫红色,装瓶方便携带,能直接饮用,绿色环保,带给消费者新奇、美好的感受。Tobacco and wine new tobacco products have obvious aroma of tobacco and sweet and sour taste of wine, rich aroma, harmonious taste, not sharp throat, overcome the defects of excessive irritation, strong bitter taste and strong spicy taste of tobacco powder used directly, Can enjoy the satisfaction and excitement brought by smokeless tobacco and a certain alcohol content; the color is clear and purple, the bottle is easy to carry, can be drunk directly, green and environmentally friendly, and brings novelty and good feelings to consumers.
实施例3Example 3
一种烟草葡萄酒新型烟草制品,采用优质烟粉、优质紫红葡萄为原料,具体包括如下步骤:A new type of tobacco wine tobacco product, using high-quality tobacco powder and high-quality purple grapes as raw materials, specifically comprising the following steps:
(1)烟草葡萄发酵酒的制备:选用果皮紫红色的葡萄,剔除病烂、病虫、生青果,用清水洗去表面污物。用滚筒式或离心式破碎将果实压破,再经除梗机去掉果梗,将破碎的葡萄果浆和优质烟粉(重量比1:20)放入不锈钢发酵罐中,加入葡萄果浆和烟粉混合物质量0.3%的复合酶(β-葡萄糖苷酶,酶活1000u/g,纤维素酶,酶活10万u/g,均为食品级,比例1:1);在纯净水中,加入水35%质量的活性干酵母、酵母营养素(NH3H2PO4)和糖(质量比1:1.5:1),不停地搅拌,直到酵母再生,冒起大量泡沫,将酵母液按葡萄果浆和烟粉混合物质量的30%加入,搅拌混合均匀,总混合物占发酵罐容积的80%;在28℃温度条件下,浸渍发酵36h后,加入水12.5%质量的单宁;检测混合物的糖度,在混合烟草葡萄浆的糖度消耗一半时,加入白砂糖,使烟草葡萄果浆混合物的含糖量达到250g/L;控制温度28℃进行发酵7天,当发酵汁含糖量达到5g/L以下时,进行皮渣分离,分离自流汁,保留;分离的皮渣,进行压榨,压榨汁保留;将自流汁和压榨汁合并,备用,称为烟草葡萄发酵酒;(1) Preparation of tobacco grape fermented wine: select grapes with purplish red skin, remove diseased and rotten fruits, diseases and insect pests, and raw green fruits, and wash away surface dirt with clear water. Use drum or centrifugal crushing to crush the fruit, then remove the stems through a destemmer, put the crushed grape pulp and high-quality tobacco powder (weight ratio 1:20) into a stainless steel fermentation tank, add grape pulp and Compound enzyme (β-glucosidase, enzyme activity 1000u/g, cellulase, enzyme activity 100,000u/g, both food grade, ratio 1:1) of 0.3% of tobacco powder mixture; in purified water, add Water 35% by mass active dry yeast, yeast nutrients (NH 3 H 2 PO 4 ) and sugar (mass ratio 1:1.5:1), stirring constantly until the yeast regenerates and produces a lot of foam, press the yeast liquid into grapes Add 30% of the mass of the mixture of fruit pulp and tobacco powder, stir and mix evenly, the total mixture accounts for 80% of the volume of the fermenter; under the temperature condition of 28 ℃, after soaking and fermenting for 36 hours, add 12.5% of the mass of tannin in water; Sugar content, when half of the sugar content of the mixed tobacco grape pulp is consumed, white granulated sugar is added to make the sugar content of the tobacco grape pulp mixture reach 250g/L; fermentation is carried out at a temperature of 28°C for 7 days, when the sugar content of the fermented juice reaches 5g/L When it is below L, separate the skin dregs, separate the artesian juice, and keep it; the separated skin dregs, squeeze it, and keep the squeezed juice; combine the artesian juice and the squeezed juice, and reserve it, which is called tobacco grape fermented wine;
(2)烟草葡萄酒萃取液的制备:将步骤1)中分离压榨后的皮渣,采用质量比10倍食用酒精放在下部,对顶部的分离压榨烟草葡萄皮渣进行45℃索氏提取3次,每次3h,合并萃取液,得到烟草葡萄酒萃取液;(2) Preparation of tobacco wine extract: put the skin pomace separated and squeezed in step 1) into the lower part with 10 times the mass ratio of edible alcohol, and perform Soxhlet extraction at 45°C for 3 times on the top separated and pressed tobacco grape skin pomace , 3 hours each time, combining the extracts to obtain tobacco wine extracts;
(3)调配:将上述步骤1)的烟草葡萄发酵酒和步骤2)的烟草葡萄酒萃取液以一定比例混合,使所得的烟草葡萄酒酒精度达到12%(v/v);(3) Blending: mix the tobacco grape fermented wine in the above step 1) with the tobacco wine extract in step 2) in a certain proportion, so that the alcohol content of the tobacco wine obtained can reach 12% (v/v);
(4)陈酿:向步骤3)的烟草葡萄调配酒中加入SO2,保持游离SO2125mg/L,并将橡木片按烟草葡萄酒质量的0.4%浸泡在烟草葡萄酒中,陈酿9个月,定期转罐去除沉淀并更换橡木片。(4) aging: add SO 2 to the tobacco grape blended wine in step 3), keep free SO 2 125mg/L, and soak the oak chips in the tobacco wine by 0.4% of the quality of the tobacco wine, age for 9 months, regularly Transfer tanks to remove sediment and replace oak chips.
(5)除菌过滤澄清化:采用模式过滤机,过滤孔径小于0.4微米,过滤除掉细菌、酵母细胞和其他杂质,使烟草葡萄酒澄清透亮,香气口感更纯粹;(5) Sterilization and filtration clarification: use a model filter with a filter aperture of less than 0.4 microns to filter out bacteria, yeast cells and other impurities, so that the tobacco wine is clear and translucent, and the aroma and taste are purer;
(6)热处理提高稳定性和促氧化:将发酵罐中的烟草葡萄酒加热到70℃,保温12min,趁热装瓶,自然冷却,达到提高烟草葡萄酒稳定性作用,因为加热有杀菌作用,防止微生物引起的混浊沉淀,破坏烟草葡萄酒中多酚氧化酶,防止烟草葡萄酒的氧化混浊。(6) Heat treatment to improve stability and promote oxidation: heat the tobacco wine in the fermenter to 70°C, keep it warm for 12 minutes, bottle it while it is hot, and cool it naturally to improve the stability of tobacco wine, because heating has a bactericidal effect and prevents microorganisms The turbid precipitation caused destroys the polyphenol oxidase in tobacco wine and prevents the oxidative turbidity of tobacco wine.
(7)成品:灌装。(7) Finished product: filling.
烟草葡萄酒新型烟草制品具有烟草香气和葡萄酒酸甜醇和轻柔协调口感,香气饱满丰富,不刺喉辣喉,克服烟粉直接使用的刺激性过大、苦涩味浓重、辛辣味强烈的缺陷,能享受无烟雾形式烟草和一定的酒精含量所带来的满足和兴奋愉悦感;色泽为清亮紫红色,装瓶方便携带,能直接饮用,绿色环保,带给消费者新奇、美好的感受。Tobacco and wine new tobacco products have tobacco aroma and wine sweet and sour taste and soft and harmonious taste. Satisfaction and excitement brought by smokeless form of tobacco and a certain alcohol content; the color is clear purple red, easy to carry in bottles, can be drunk directly, green and environmentally friendly, and brings novelty and good feelings to consumers.
试验例1:感官效果评价Test Example 1: Sensory Effect Evaluation
将实施例1-3制备的烟草葡萄酒新型烟草制品进行感官评价,对照组a为烟草粉末制品,对照组b只为烟草、糖和一定浓度食用酒精发酵制品,不添加β-葡萄糖苷酶、纤维素酶、活性干酵母等,也不含烟草渣酒精萃取液,单纯的烟草发酵酒。The new tobacco wine tobacco products prepared in Examples 1-3 were sensory evaluated. The control group a was tobacco powder products, and the control group b was only fermented products of tobacco, sugar and a certain concentration of edible alcohol, without adding β-glucosidase and fiber Suzyme, active dry yeast, etc., and does not contain tobacco residue alcohol extract, pure tobacco fermented wine.
结果表明:本发明以烟草、葡萄为原料,经β-葡萄糖苷酶、纤维素酶复合发酵生香,再经活性干酵母发酵而成烟草葡萄酒,克服烟粉直接使用的刺激性过大、苦涩味浓重、辛辣味强烈的缺陷。与对照组b单纯的烟草发酵酒相比,烟草葡萄酒新型烟草制品,保留烟草、葡萄的功能性成分,含有多种类黄酮类物质、花青素,具有强抗氧化、抗突变、减轻肝机能障碍、保护心血管、降低血压等功能,营养丰富。烟草葡萄酒新型烟草制品,烟草葡萄果浆等经复合酒精发酵、混合酒精萃取以及通过橡木片浸渍陈酿后,具有烟草特征香气和葡萄酒酸甜醇和轻柔口感,香气饱满丰富,不刺喉辣喉,能享受无烟雾形式烟草和一定的酒精含量所带来的满足和兴奋愉悦感;色泽为清亮紫红色,装瓶方便携带,能直接饮用,绿色环保,带给消费者新奇、美好的感受。The results show that the present invention uses tobacco and grapes as raw materials, produces aroma through compound fermentation of β-glucosidase and cellulase, and then ferments with active dry yeast to form tobacco wine, which overcomes the excessive irritation and bitterness of direct use of tobacco powder. Defects of strong flavor and strong spicy taste. Compared with the simple tobacco fermented wine in the control group b, the tobacco wine new tobacco products retain the functional components of tobacco and grapes, contain a variety of flavonoids and anthocyanins, have strong antioxidant, anti-mutation, and alleviate liver dysfunction , protect cardiovascular, lower blood pressure and other functions, rich in nutrition. Tobacco wine new tobacco products, tobacco grape pulp, etc., after compound alcohol fermentation, mixed alcohol extraction and oak chip impregnation and aging, have the characteristic aroma of tobacco, sweet and sour wine and soft taste, full and rich aroma, not pungent throat, can Enjoy the satisfaction and excitement brought by smokeless tobacco and a certain alcohol content; the color is clear and purple, the bottle is easy to carry, can be drunk directly, green and environmentally friendly, and brings novelty and good feelings to consumers.
表1.烟草葡萄酒新型烟草制品感官效果评价Table 1. Evaluation of sensory effects of new tobacco products from tobacco and wine
以上所述,仅为本发明的具体实施方式,但本发明的保护范围并不局限于此,任何属于本技术领域的技术人员在本发明揭露的技术范围内,可轻易想到的变化或替换,都应涵盖在本发明的保护范围之内。因此,本发明的保护范围应该以权利要求的保护范围为准。The above is only a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, any changes or substitutions that can be easily imagined by those skilled in the art within the technical scope disclosed in the present invention, All should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be determined by the protection scope of the claims.
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