CN106367205A - Preparing method and application of black currant fruit anthocyanin compound - Google Patents
Preparing method and application of black currant fruit anthocyanin compound Download PDFInfo
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- CN106367205A CN106367205A CN201610929440.4A CN201610929440A CN106367205A CN 106367205 A CN106367205 A CN 106367205A CN 201610929440 A CN201610929440 A CN 201610929440A CN 106367205 A CN106367205 A CN 106367205A
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- 235000016954 Ribes hudsonianum Nutrition 0.000 title claims abstract description 66
- 240000001890 Ribes hudsonianum Species 0.000 title claims abstract description 66
- 235000001466 Ribes nigrum Nutrition 0.000 title claims abstract description 66
- 238000000034 method Methods 0.000 title claims abstract description 18
- -1 anthocyanin compound Chemical class 0.000 title abstract description 5
- 229930002877 anthocyanin Natural products 0.000 title abstract 4
- 235000010208 anthocyanin Nutrition 0.000 title abstract 4
- 239000004410 anthocyanin Substances 0.000 title abstract 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000003205 fragrance Substances 0.000 claims abstract description 20
- 238000000638 solvent extraction Methods 0.000 claims abstract description 16
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 13
- 102000006995 beta-Glucosidase Human genes 0.000 claims abstract description 13
- 108010047754 beta-Glucosidase Proteins 0.000 claims abstract description 13
- 235000009508 confectionery Nutrition 0.000 claims abstract description 12
- 239000000706 filtrate Substances 0.000 claims description 25
- 238000001914 filtration Methods 0.000 claims description 19
- 239000002304 perfume Substances 0.000 claims description 18
- 238000002360 preparation method Methods 0.000 claims description 17
- 238000000855 fermentation Methods 0.000 claims description 15
- 230000004151 fermentation Effects 0.000 claims description 15
- 239000003546 flue gas Substances 0.000 claims description 12
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 10
- 235000013305 food Nutrition 0.000 claims description 10
- 238000000605 extraction Methods 0.000 claims description 9
- 238000004108 freeze drying Methods 0.000 claims description 8
- 239000002244 precipitate Substances 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 244000061176 Nicotiana tabacum Species 0.000 claims description 4
- 235000019504 cigarettes Nutrition 0.000 abstract description 22
- 239000000463 material Substances 0.000 abstract description 14
- 241000208125 Nicotiana Species 0.000 abstract description 9
- 235000019505 tobacco product Nutrition 0.000 abstract description 6
- 238000000889 atomisation Methods 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 4
- 239000000779 smoke Substances 0.000 abstract description 4
- 229920002521 macromolecule Polymers 0.000 abstract description 3
- 239000002085 irritant Substances 0.000 abstract description 2
- 231100000021 irritant Toxicity 0.000 abstract description 2
- 230000007794 irritation Effects 0.000 abstract 2
- 238000011049 filling Methods 0.000 abstract 1
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- 230000000717 retained effect Effects 0.000 abstract 1
- 238000009777 vacuum freeze-drying Methods 0.000 abstract 1
- 238000012856 packing Methods 0.000 description 9
- 230000008859 change Effects 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 230000001737 promoting effect Effects 0.000 description 6
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 229960005070 ascorbic acid Drugs 0.000 description 4
- 239000003610 charcoal Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 3
- 239000003595 mist Substances 0.000 description 3
- 235000002357 Ribes grossularia Nutrition 0.000 description 2
- 244000171263 Ribes grossularia Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 229930014669 anthocyanidin Natural products 0.000 description 2
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 2
- 235000008758 anthocyanidins Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
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- 241000220483 Ribes Species 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000134890 Saxifragales Species 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- 238000012387 aerosolization Methods 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000020279 black tea Nutrition 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011285 coke tar Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 235000019990 fruit wine Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
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- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 210000000614 rib Anatomy 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
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- 230000000391 smoking effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 150000008495 β-glucosides Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/0003—Compounds of unspecified constitution defined by the chemical reaction for their preparation
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/302—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances by natural substances obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/305—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances of undetermined constitution characterised by their preparation
Abstract
The invention provides a preparing method and application of a black currant fruit anthocyanin compound. The preparing method includes the steps that black currant fruit is used as a raw material, smashed and put into a pot, beta-glucosidase is used for fermenting and generating fragrance, filter liquor is retained, after low-temperature procedure pressurizing, solvent extraction is carried out on filter residue multiple times, all filter liquor is converged, and cryoconcentration alcohol precipitation and vacuum freeze drying are carried out, so that fragrance is purer and richer, irritant macromolecular substances and impurities are reduced, and irritation is reduced. The black currant fruit anthocyanin compound is applied to cigarette cut tobacco to harmonize smoke, so that fragrance in the smoke is richer, irritation and offensive odor are reduced, and the mouthfeel is improved. The black currant fruit anthocyanin compound is applied to the novel tobacco product atomization filling material, the fragrance is enriched, the faint sweet aroma is increased, and the comfortable mouthfeel is improved.
Description
Technical field
The present invention relates to tobacco additive agent technical field, the system containing anthocyan complex for specifically a kind of black currant fruit
Preparation Method and its application.
Background technology
Smoking and health are always the main bugbear that Nicotiana tabacum L. circle and medical and health interface are faced, and are also today's society extensive concern
Focus.Select suitable plant extract to be added in low-coke tar cigarette, be by a kind of effectively way of Medicated cigarette compensating aroma
Footpath, plays the role of important to the quality improving Nicotiana tabacum L..In cigarette product, mostly the additive of application is Nicotiana tabacum L., natural plants at present
Class extract, these extracts are typically obtained by water, alcohol extraction or vapor distillation, the improvement journey to cigarette quality after interpolation
Degree is limited, still the irritant big, defect such as miscellaneous QI is many.
Black currant (munakka), also known as black tea, Saxifragales Fructus Camelliae sinensis section genus ribes, small shrub class, distribution
In Europe, the especially Finland in Northern Europe and Sweden, the provinces and regions such as China Heilungkiang, Jilin and Inner Mongol introducing and planting in a large number.Its
Mature fruit is red bright small berries, interior rich in vitamin c, anthocyanidin.The fruit tart flavour of its red cultivar is stronger, typically
It is used for processing.Black currant contains the materials such as very abundant vitamin c, anthocyanidin, phenols, has good health-care effect, energy
Effectively prevention gout, anemia, edema, laryngopharyngeal diseasess, cough etc..Fruit can be processed and make the product such as fruit juice, fruit jam, fruit jelly, fruit wine
Product.
After the crushing of black currant fruit, take beta-glucosidase enzyme fermentation raw fragrant, retain filtrate, by filtering residue through low temperature procedure
It is pressurized multiple solvent extraction, remerge all filtrates, cryoconcentration precipitate with ethanol, vacuum lyophilization, its fragrance is purer, abundant,
Zest macromolecular substances, impurity reduce, and zest reduces.It is applied in cigarette shreds, coordinates flue gas, make in flue gas fragrance more
Abundant, the slightly fresh and sweet perfume (or spice) of fruit, promoting or transferring cigarette perfume, reduces and stimulates and reduce miscellaneous QI, improve mouthfeel;It is applied to novel tobacco product mist
Change in packing material, abundant fragrance, increase the fresh and sweet perfume (or spice) of fruit, increase comfortable mouthfeel and have not been reported.
Content of the invention
It is an object of the invention to provide a kind of preparation method and applications of black currant fruit anthocyan complex, with red
Gooseberry fruit is raw material, puts in tank after crushing, takes beta-glucosidase enzyme fermentation raw fragrant, retains filtrate, by filtering residue through low temperature
Program is pressurized multiple solvent extraction, remerges all filtrates, cryoconcentration precipitate with ethanol, vacuum lyophilization, obtains containing of purification
Anthocyan complex in the black currant fruit of aroma substance, its fragrance is purer, abundant, zest macromolecular substances, impurity
Tail off, zest reduces.It is applied in Medicated cigarette, coordinates with flue gas, make the fragrance of flue gas more rich, reduce and stimulate, reduce miscellaneous QI,
Improve aesthetic quality;It is applied in novel tobacco product propellant, abundant fragrance, increase noble rose suggestion, promoting or transferring cigarette perfume.
Above-mentioned purpose is achieved through the following technical solutions:
The preparation method of anthocyan complex in a kind of black currant fruit, comprises the following steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:20~50, and press red
0.05~0.3% addition beta-glucosidase of gooseberry fruit quality, stirs, under 25~45 DEG C, ph natural conditions, sends out
Ferment 4~18h, sucking filtration, retain filtrate, obtain black currant fruit fermentation liquid;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag
In, put into program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:20~50, be pressurized multiple solvent through program
Extraction, collects filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration, sucking filtration, by filtrate through vacuum lyophilization, obtain black currant fruit
Anthocyan complex in reality.
Further, step 1) described in beta-glucosidase be food stage, enzyme activity 100u~10000u/g.
Further, step 2) described in ethanol be food stage, 95% ethanol;
Further, described step 2) Program supercharging solvent extraction condition be: 5~45 DEG C of temperature, air source pressure be 1
~8bar;Setting extraction temperature is 15~28 DEG C, and pressure is adjusted to every 1~4 minute boosting 0.1bar, rises to 8bar and keeps 2~4
Hour, circulate 1~3 time.
The present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, and its feature exists
In: described black currant fruit anthocyan complex in mass ratio 0.005~0.02% is added in tobacco shred, with flue gas association
Adjust, abundant fragrance, increase fruit is fragrant and sweet, reduce and stimulate and reduce miscellaneous QI, improve aesthetic quality.
The present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, and its feature exists
In: described black currant fruit anthocyan complex in mass ratio 0.005~0.02% is added to electrical heating or charcoal heating is new
Use in the atomization packing material of type tobacco product, the fragrance of abundant novel tobacco product, increase fruit is fragrant and sweet, promoting or transferring cigarette perfume.
Beneficial effects of the present invention:
1) anthocyan complex in black currant fruit provided by the present invention, with black currant fruit as raw material, take β-
Glucoside enzyme fermentation is raw fragrant, decomposes, transformed cell wall material is aroma substance or flavor precursor, and discharges more small molecules
Bioactive ingredients, macromole pungent reduces;
2) filtering residue after its fermentation, loads in micropore filter bag, is pressurized multiple solvent extraction through low temperature procedure, production process is clear
Clean, pre-filtering extract;Between 5~45 DEG C, relatively low extraction temperature makes to the more sensitive bioactive ingredients of temperature such as temperature
Abundant vitamin c, the property of anthocyan complex do not change, and vitamin c, anthocyan complex have to human body
Benefit, energy antioxidation, defying age, the oxidation of opposing flue gas free radical;Extraction cycle the dynamic system being obtained by pressure programming with
Between static systems alternately, in dynamic phase in the early stage, pressure is to produce in solvent cyclic process, this ensure that
The infiltration of effect, simultaneously, it is to avoid the formation of solvent leak and solute supersaturation;Extraction process pressure programming, product quality is reappeared
Property good good stability, solvent loss is minimum, reduces raw material memory space, saves manpower financial capacity.
3) all filtrates of merging, cryoconcentration precipitate with ethanol, vacuum lyophilization, cryoconcentration and lyophilization are preservation life
The living property of material, prepared black currant fruit anthocyan complex, its fragrance is purer, abundant, zest macromole
Material, impurity reduce, and zest reduces.
4) it is applied in cigarette shreds, make fragrance in flue gas more rich, promoting or transferring cigarette perfume, reduces and stimulates and reduce miscellaneous QI, change
Kind mouthfeel;It is applied in novel tobacco product atomization packing material, abundant fragrance, increase fruit is fragrant and sweet, improves throat's comfortableness.
5) present invention process mild condition, processing step is easy to operate, and product yield is high, and raw material used has natural environmental-protective
Harmless the advantages of, there is larger prospects for commercial application.
Specific embodiment
Below in conjunction with specific embodiment, the technical scheme in the present invention is clearly and completely described.
Embodiment 1
A kind of preparation method of black currant fruit anthocyan complex, comprises the following steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:20, and press black currant
0.05% addition beta-glucosidase of fruit quality, stirs, and under 40 DEG C, ph natural conditions, ferment 6h, and sucking filtration is protected
Reserved filtrate, obtains black currant fruit fermentation liquid;
Described beta-glucosidase, food stage, enzyme activity 10000u/g;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag
In, put into program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:20, be pressurized solvent extraction through program, receive
Collection filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration to relative density 1.31, sucking filtration, filtrate is done through vacuum freezing
Dry, obtain anthocyan complex in black currant fruit.
Described ethanol is food stage, 95% ethanol;
Described step 2) Program supercharging solvent extraction condition be: 25 DEG C of temperature, air source pressure be 6bar, extraction temperature
18 DEG C, pressure is adjusted to every 2 minutes boosting 0.1bar, rises to 8bar and keeps 2 hours, circulates 2 times.
The embodiment of the present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, its
It is characterised by: described black currant fruit anthocyan complex in mass ratio 0.005% is added in tobacco shred, with flue gas association
Adjust, abundant fragrance, slightly fruit are fragrant and sweet, reduce and stimulate and reduce miscellaneous QI, improve aesthetic quality.
Embodiment 2
A kind of preparation method of black currant fruit anthocyan complex, comprises the following steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:30, and press black currant
0.1% addition beta-glucosidase of fruit quality, stirs, and under 42 DEG C, ph natural conditions, ferment 8h, and sucking filtration retains
Filtrate, obtains black currant fruit fermentation liquid;
Described beta-glucosidase, food stage, enzyme activity 5000u/g;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag
In, put into program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:35, be pressurized solvent extraction through program, receive
Collection filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration, sucking filtration, by filtrate through vacuum lyophilization, obtain black currant fruit
Anthocyan complex in reality.
Described ethanol is food stage, 95% ethanol;
Described step 2) Program supercharging solvent extraction condition be: 10 DEG C of temperature, air source pressure be 8bar, extraction temperature
For 22 DEG C, pressure is adjusted to every 3 minutes boosting 0.1bar, rises to 8bar and keeps 3 hours, circulates 2 times.
The embodiment of the present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, its
It is characterised by: anthocyan complex in described black currant fruit in mass ratio 0.005% is added to the mist of electrical heating Medicated cigarette
Change in packing material and use, enrich its fragrance, with the fresh and sweet perfume (or spice) of fruit, promoting or transferring cigarette perfume.
Embodiment 3
A kind of preparation method of black currant fruit anthocyan complex, comprises the following steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:40, and press black currant
0.2% addition beta-glucosidase of fruit quality, stirs, and under 45 DEG C, ph natural conditions, ferment 12h, and sucking filtration is protected
Reserved filtrate, obtains black currant fruit fermentation liquid;
Described beta-glucosidase, food stage, enzyme activity 1000u/g;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag
In, put into program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:40, through program supercharging multiple solvent extraction
Take, collect filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration, sucking filtration, by filtrate through vacuum lyophilization, obtain black currant fruit
Anthocyan complex in reality.
Described ethanol is food stage, 95% ethanol;
Described step 2) Program supercharging solvent extraction condition be: 40 DEG C of temperature, air source pressure be 5bar, extraction temperature
For 28 DEG C, pressure is adjusted to every 4 minutes boosting 0.1bar, rises to 8bar and keeps 4 hours, circulates 3 times.
The embodiment of the present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, its
It is characterised by: anthocyan complex in described black currant fruit in mass ratio 0.005% is added to the mist that charcoal heats Medicated cigarette
Change in packing material and use, enrich its fragrance, increase the fresh and sweet perfume (or spice) of fruit, promoting or transferring cigarette perfume.
Test example 1: sensorial effects test
Black currant fruit anthocyan complex prepared by embodiment 1, in mass ratio 0.005%, 0.01%, 0.02%
It is added in tobacco shred (b class).Then tobacco shred is loaded hermetic bag, place 80 DEG C of constant temperature 30min in an oven, suitable to moisture,
Roll into cigarette, put in climatic chamber (22 DEG C+1, relative humidity be 60%+2%) and smooth 48h, ask expert to smoke panel test group
Smoke panel test.
Result shows (being shown in Table 1), and black currant fruit anthocyan complex in mass ratio 0.005% and 0.01% is added to
In tobacco shred, can coordinate with flue gas, make tobacco shred color and luster more natural uniform, be rich in gloss, fragrance is more rich, slightly the fresh and sweet perfume (or spice) of fruit,
Reduce and stimulate and reduce miscellaneous QI, improve aesthetic quality.
Table 1. black currant fruit anthocyan complex is added to the sensory evaluation in tobacco shred
Black currant fruit anthocyan complex in mass ratio 0.005% prepared by embodiment 2 is added to electrical heating volume
In the atomization packing material of cigarette, abundant cigarette perfume, the slightly fresh and sweet effect (being shown in Table 2) that is fragrant, increasing throat's comfortableness of fruit can be played.
The sense organ that table 2. black currant fruit anthocyan complex is added in the atomization packing material of electrical heating Medicated cigarette is commented
Valency
Black currant fruit anthocyan complex in mass ratio 0.005% prepared by embodiment 3 is added to charcoal heating volume
In aerosolization packing material, can play and coordinate flue gas, abundant cigarette perfume, the effect (being shown in Table 3) of raising flue gas quality.
Table 3. black currant fruit anthocyan complex is added to charcoal and heats the sensory evaluation that Medicated cigarette is atomized in packing material
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any
Belong to those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in, all answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be defined by scope of the claims.
Claims (5)
1. in a kind of black currant fruit the preparation method of anthocyan complex it is characterised in that comprising the steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:20~50, and press black currant
0.05~0.3% addition beta-glucosidase of fruit quality, stirs, under 25~45 DEG C, ph natural conditions, fermentation 4
~18h, sucking filtration, retain filtrate, obtain black currant fruit fermentation liquid;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag in, put
Enter program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:20~50, be pressurized multiple solvent extraction through program,
Collect filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration, sucking filtration, by filtrate through vacuum lyophilization, obtain in black currant fruit
Anthocyan complex.
2. in black currant fruit as claimed in claim 1 anthocyan complex preparation method it is characterised in that: step 1)
Described in beta-glucosidase be food stage, enzyme activity 100u~10000u/g.
3. in black currant fruit as claimed in claim 1 anthocyan complex preparation method it is characterised in that: step 2)
Described in ethanol be food stage, 95% ethanol.
4. in black currant fruit as claimed in claim 1 anthocyan complex preparation method it is characterised in that: described step
Rapid 2) Program supercharging solvent extraction condition is: 25 DEG C of temperature, air source pressure is 6bar, 18 DEG C of extraction temperature, and pressure is adjusted
For every 2 minutes boosting 0.1bar, rise to 8bar and keep 2 hours, circulate 2 times.
5. the application of the black currant fruit anthocyan complex of any one of a kind of claim 1-4 preparation, its feature exists
In: described black currant fruit anthocyan complex in mass ratio 0.005% is added in tobacco shred, coordinates with flue gas, enrich
Fragrance, slightly fruit are fragrant and sweet, reduce and stimulate and reduce miscellaneous QI, improve aesthetic quality.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN107723092A (en) * | 2017-11-29 | 2018-02-23 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications with fragrant and sweet fermentation angelica extract |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101999648A (en) * | 2010-10-11 | 2011-04-06 | 宝鸡市虹源生物科技有限公司 | Extraction method of anthocyanin and original anthocyanin components in cranberry fruits |
CN104489916A (en) * | 2014-12-02 | 2015-04-08 | 云南中烟工业有限责任公司 | Currant extract as well as preparation method and application thereof to cigarette |
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CN101999648A (en) * | 2010-10-11 | 2011-04-06 | 宝鸡市虹源生物科技有限公司 | Extraction method of anthocyanin and original anthocyanin components in cranberry fruits |
CN104489916A (en) * | 2014-12-02 | 2015-04-08 | 云南中烟工业有限责任公司 | Currant extract as well as preparation method and application thereof to cigarette |
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CN107723092A (en) * | 2017-11-29 | 2018-02-23 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications with fragrant and sweet fermentation angelica extract |
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