CN106367205A - Preparing method and application of black currant fruit anthocyanin compound - Google Patents

Preparing method and application of black currant fruit anthocyanin compound Download PDF

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Publication number
CN106367205A
CN106367205A CN201610929440.4A CN201610929440A CN106367205A CN 106367205 A CN106367205 A CN 106367205A CN 201610929440 A CN201610929440 A CN 201610929440A CN 106367205 A CN106367205 A CN 106367205A
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CN
China
Prior art keywords
black currant
fruit
currant fruit
anthocyan
complex
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610929440.4A
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Chinese (zh)
Inventor
魏敏
陈义坤
程炳发
罗诚浩
宋旭艳
岳海波
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China Tobacco Hunan Industrial Co Ltd
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China Tobacco Hunan Industrial Co Ltd
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Priority to CN201610929440.4A priority Critical patent/CN106367205A/en
Publication of CN106367205A publication Critical patent/CN106367205A/en
Pending legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00Essential oils; Perfumes
    • C11B9/0003Compounds of unspecified constitution defined by the chemical reaction for their preparation
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/28Treatment of tobacco products or tobacco substitutes by chemical substances
    • A24B15/30Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
    • A24B15/302Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances by natural substances obtained from animals or plants
    • AHUMAN NECESSITIES
    • A24TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24BMANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18Treatment of tobacco products or tobacco substitutes
    • A24B15/28Treatment of tobacco products or tobacco substitutes by chemical substances
    • A24B15/30Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
    • A24B15/305Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances of undetermined constitution characterised by their preparation

Abstract

The invention provides a preparing method and application of a black currant fruit anthocyanin compound. The preparing method includes the steps that black currant fruit is used as a raw material, smashed and put into a pot, beta-glucosidase is used for fermenting and generating fragrance, filter liquor is retained, after low-temperature procedure pressurizing, solvent extraction is carried out on filter residue multiple times, all filter liquor is converged, and cryoconcentration alcohol precipitation and vacuum freeze drying are carried out, so that fragrance is purer and richer, irritant macromolecular substances and impurities are reduced, and irritation is reduced. The black currant fruit anthocyanin compound is applied to cigarette cut tobacco to harmonize smoke, so that fragrance in the smoke is richer, irritation and offensive odor are reduced, and the mouthfeel is improved. The black currant fruit anthocyanin compound is applied to the novel tobacco product atomization filling material, the fragrance is enriched, the faint sweet aroma is increased, and the comfortable mouthfeel is improved.

Description

A kind of preparation method and applications of black currant fruit anthocyan complex
Technical field
The present invention relates to tobacco additive agent technical field, the system containing anthocyan complex for specifically a kind of black currant fruit Preparation Method and its application.
Background technology
Smoking and health are always the main bugbear that Nicotiana tabacum L. circle and medical and health interface are faced, and are also today's society extensive concern Focus.Select suitable plant extract to be added in low-coke tar cigarette, be by a kind of effectively way of Medicated cigarette compensating aroma Footpath, plays the role of important to the quality improving Nicotiana tabacum L..In cigarette product, mostly the additive of application is Nicotiana tabacum L., natural plants at present Class extract, these extracts are typically obtained by water, alcohol extraction or vapor distillation, the improvement journey to cigarette quality after interpolation Degree is limited, still the irritant big, defect such as miscellaneous QI is many.
Black currant (munakka), also known as black tea, Saxifragales Fructus Camelliae sinensis section genus ribes, small shrub class, distribution In Europe, the especially Finland in Northern Europe and Sweden, the provinces and regions such as China Heilungkiang, Jilin and Inner Mongol introducing and planting in a large number.Its Mature fruit is red bright small berries, interior rich in vitamin c, anthocyanidin.The fruit tart flavour of its red cultivar is stronger, typically It is used for processing.Black currant contains the materials such as very abundant vitamin c, anthocyanidin, phenols, has good health-care effect, energy Effectively prevention gout, anemia, edema, laryngopharyngeal diseasess, cough etc..Fruit can be processed and make the product such as fruit juice, fruit jam, fruit jelly, fruit wine Product.
After the crushing of black currant fruit, take beta-glucosidase enzyme fermentation raw fragrant, retain filtrate, by filtering residue through low temperature procedure It is pressurized multiple solvent extraction, remerge all filtrates, cryoconcentration precipitate with ethanol, vacuum lyophilization, its fragrance is purer, abundant, Zest macromolecular substances, impurity reduce, and zest reduces.It is applied in cigarette shreds, coordinates flue gas, make in flue gas fragrance more Abundant, the slightly fresh and sweet perfume (or spice) of fruit, promoting or transferring cigarette perfume, reduces and stimulates and reduce miscellaneous QI, improve mouthfeel;It is applied to novel tobacco product mist Change in packing material, abundant fragrance, increase the fresh and sweet perfume (or spice) of fruit, increase comfortable mouthfeel and have not been reported.
Content of the invention
It is an object of the invention to provide a kind of preparation method and applications of black currant fruit anthocyan complex, with red Gooseberry fruit is raw material, puts in tank after crushing, takes beta-glucosidase enzyme fermentation raw fragrant, retains filtrate, by filtering residue through low temperature Program is pressurized multiple solvent extraction, remerges all filtrates, cryoconcentration precipitate with ethanol, vacuum lyophilization, obtains containing of purification Anthocyan complex in the black currant fruit of aroma substance, its fragrance is purer, abundant, zest macromolecular substances, impurity Tail off, zest reduces.It is applied in Medicated cigarette, coordinates with flue gas, make the fragrance of flue gas more rich, reduce and stimulate, reduce miscellaneous QI, Improve aesthetic quality;It is applied in novel tobacco product propellant, abundant fragrance, increase noble rose suggestion, promoting or transferring cigarette perfume.
Above-mentioned purpose is achieved through the following technical solutions:
The preparation method of anthocyan complex in a kind of black currant fruit, comprises the following steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:20~50, and press red 0.05~0.3% addition beta-glucosidase of gooseberry fruit quality, stirs, under 25~45 DEG C, ph natural conditions, sends out Ferment 4~18h, sucking filtration, retain filtrate, obtain black currant fruit fermentation liquid;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag In, put into program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:20~50, be pressurized multiple solvent through program Extraction, collects filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration, sucking filtration, by filtrate through vacuum lyophilization, obtain black currant fruit Anthocyan complex in reality.
Further, step 1) described in beta-glucosidase be food stage, enzyme activity 100u~10000u/g.
Further, step 2) described in ethanol be food stage, 95% ethanol;
Further, described step 2) Program supercharging solvent extraction condition be: 5~45 DEG C of temperature, air source pressure be 1 ~8bar;Setting extraction temperature is 15~28 DEG C, and pressure is adjusted to every 1~4 minute boosting 0.1bar, rises to 8bar and keeps 2~4 Hour, circulate 1~3 time.
The present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, and its feature exists In: described black currant fruit anthocyan complex in mass ratio 0.005~0.02% is added in tobacco shred, with flue gas association Adjust, abundant fragrance, increase fruit is fragrant and sweet, reduce and stimulate and reduce miscellaneous QI, improve aesthetic quality.
The present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, and its feature exists In: described black currant fruit anthocyan complex in mass ratio 0.005~0.02% is added to electrical heating or charcoal heating is new Use in the atomization packing material of type tobacco product, the fragrance of abundant novel tobacco product, increase fruit is fragrant and sweet, promoting or transferring cigarette perfume.
Beneficial effects of the present invention:
1) anthocyan complex in black currant fruit provided by the present invention, with black currant fruit as raw material, take β- Glucoside enzyme fermentation is raw fragrant, decomposes, transformed cell wall material is aroma substance or flavor precursor, and discharges more small molecules Bioactive ingredients, macromole pungent reduces;
2) filtering residue after its fermentation, loads in micropore filter bag, is pressurized multiple solvent extraction through low temperature procedure, production process is clear Clean, pre-filtering extract;Between 5~45 DEG C, relatively low extraction temperature makes to the more sensitive bioactive ingredients of temperature such as temperature Abundant vitamin c, the property of anthocyan complex do not change, and vitamin c, anthocyan complex have to human body Benefit, energy antioxidation, defying age, the oxidation of opposing flue gas free radical;Extraction cycle the dynamic system being obtained by pressure programming with Between static systems alternately, in dynamic phase in the early stage, pressure is to produce in solvent cyclic process, this ensure that The infiltration of effect, simultaneously, it is to avoid the formation of solvent leak and solute supersaturation;Extraction process pressure programming, product quality is reappeared Property good good stability, solvent loss is minimum, reduces raw material memory space, saves manpower financial capacity.
3) all filtrates of merging, cryoconcentration precipitate with ethanol, vacuum lyophilization, cryoconcentration and lyophilization are preservation life The living property of material, prepared black currant fruit anthocyan complex, its fragrance is purer, abundant, zest macromole Material, impurity reduce, and zest reduces.
4) it is applied in cigarette shreds, make fragrance in flue gas more rich, promoting or transferring cigarette perfume, reduces and stimulates and reduce miscellaneous QI, change Kind mouthfeel;It is applied in novel tobacco product atomization packing material, abundant fragrance, increase fruit is fragrant and sweet, improves throat's comfortableness.
5) present invention process mild condition, processing step is easy to operate, and product yield is high, and raw material used has natural environmental-protective Harmless the advantages of, there is larger prospects for commercial application.
Specific embodiment
Below in conjunction with specific embodiment, the technical scheme in the present invention is clearly and completely described.
Embodiment 1
A kind of preparation method of black currant fruit anthocyan complex, comprises the following steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:20, and press black currant 0.05% addition beta-glucosidase of fruit quality, stirs, and under 40 DEG C, ph natural conditions, ferment 6h, and sucking filtration is protected Reserved filtrate, obtains black currant fruit fermentation liquid;
Described beta-glucosidase, food stage, enzyme activity 10000u/g;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag In, put into program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:20, be pressurized solvent extraction through program, receive Collection filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration to relative density 1.31, sucking filtration, filtrate is done through vacuum freezing Dry, obtain anthocyan complex in black currant fruit.
Described ethanol is food stage, 95% ethanol;
Described step 2) Program supercharging solvent extraction condition be: 25 DEG C of temperature, air source pressure be 6bar, extraction temperature 18 DEG C, pressure is adjusted to every 2 minutes boosting 0.1bar, rises to 8bar and keeps 2 hours, circulates 2 times.
The embodiment of the present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, its It is characterised by: described black currant fruit anthocyan complex in mass ratio 0.005% is added in tobacco shred, with flue gas association Adjust, abundant fragrance, slightly fruit are fragrant and sweet, reduce and stimulate and reduce miscellaneous QI, improve aesthetic quality.
Embodiment 2
A kind of preparation method of black currant fruit anthocyan complex, comprises the following steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:30, and press black currant 0.1% addition beta-glucosidase of fruit quality, stirs, and under 42 DEG C, ph natural conditions, ferment 8h, and sucking filtration retains Filtrate, obtains black currant fruit fermentation liquid;
Described beta-glucosidase, food stage, enzyme activity 5000u/g;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag In, put into program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:35, be pressurized solvent extraction through program, receive Collection filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration, sucking filtration, by filtrate through vacuum lyophilization, obtain black currant fruit Anthocyan complex in reality.
Described ethanol is food stage, 95% ethanol;
Described step 2) Program supercharging solvent extraction condition be: 10 DEG C of temperature, air source pressure be 8bar, extraction temperature For 22 DEG C, pressure is adjusted to every 3 minutes boosting 0.1bar, rises to 8bar and keeps 3 hours, circulates 2 times.
The embodiment of the present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, its It is characterised by: anthocyan complex in described black currant fruit in mass ratio 0.005% is added to the mist of electrical heating Medicated cigarette Change in packing material and use, enrich its fragrance, with the fresh and sweet perfume (or spice) of fruit, promoting or transferring cigarette perfume.
Embodiment 3
A kind of preparation method of black currant fruit anthocyan complex, comprises the following steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:40, and press black currant 0.2% addition beta-glucosidase of fruit quality, stirs, and under 45 DEG C, ph natural conditions, ferment 12h, and sucking filtration is protected Reserved filtrate, obtains black currant fruit fermentation liquid;
Described beta-glucosidase, food stage, enzyme activity 1000u/g;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag In, put into program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:40, through program supercharging multiple solvent extraction Take, collect filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration, sucking filtration, by filtrate through vacuum lyophilization, obtain black currant fruit Anthocyan complex in reality.
Described ethanol is food stage, 95% ethanol;
Described step 2) Program supercharging solvent extraction condition be: 40 DEG C of temperature, air source pressure be 5bar, extraction temperature For 28 DEG C, pressure is adjusted to every 4 minutes boosting 0.1bar, rises to 8bar and keeps 4 hours, circulates 3 times.
The embodiment of the present invention also provides a kind of application of the black currant fruit anthocyan complex of said method preparation, its It is characterised by: anthocyan complex in described black currant fruit in mass ratio 0.005% is added to the mist that charcoal heats Medicated cigarette Change in packing material and use, enrich its fragrance, increase the fresh and sweet perfume (or spice) of fruit, promoting or transferring cigarette perfume.
Test example 1: sensorial effects test
Black currant fruit anthocyan complex prepared by embodiment 1, in mass ratio 0.005%, 0.01%, 0.02% It is added in tobacco shred (b class).Then tobacco shred is loaded hermetic bag, place 80 DEG C of constant temperature 30min in an oven, suitable to moisture, Roll into cigarette, put in climatic chamber (22 DEG C+1, relative humidity be 60%+2%) and smooth 48h, ask expert to smoke panel test group Smoke panel test.
Result shows (being shown in Table 1), and black currant fruit anthocyan complex in mass ratio 0.005% and 0.01% is added to In tobacco shred, can coordinate with flue gas, make tobacco shred color and luster more natural uniform, be rich in gloss, fragrance is more rich, slightly the fresh and sweet perfume (or spice) of fruit, Reduce and stimulate and reduce miscellaneous QI, improve aesthetic quality.
Table 1. black currant fruit anthocyan complex is added to the sensory evaluation in tobacco shred
Black currant fruit anthocyan complex in mass ratio 0.005% prepared by embodiment 2 is added to electrical heating volume In the atomization packing material of cigarette, abundant cigarette perfume, the slightly fresh and sweet effect (being shown in Table 2) that is fragrant, increasing throat's comfortableness of fruit can be played.
The sense organ that table 2. black currant fruit anthocyan complex is added in the atomization packing material of electrical heating Medicated cigarette is commented Valency
Black currant fruit anthocyan complex in mass ratio 0.005% prepared by embodiment 3 is added to charcoal heating volume In aerosolization packing material, can play and coordinate flue gas, abundant cigarette perfume, the effect (being shown in Table 3) of raising flue gas quality.
Table 3. black currant fruit anthocyan complex is added to charcoal and heats the sensory evaluation that Medicated cigarette is atomized in packing material
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any Belong to those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in, all answer It is included within the scope of the present invention.Therefore, protection scope of the present invention should be defined by scope of the claims.

Claims (5)

1. in a kind of black currant fruit the preparation method of anthocyan complex it is characterised in that comprising the steps:
1) ferment raw perfume: black currant fruit is crushed, loads in tank, add the water that mass ratio is 1:20~50, and press black currant 0.05~0.3% addition beta-glucosidase of fruit quality, stirs, under 25~45 DEG C, ph natural conditions, fermentation 4 ~18h, sucking filtration, retain filtrate, obtain black currant fruit fermentation liquid;
2) low temperature procedure be pressurized multiple solvent extraction: by step 1) in black currant fruit fermentation filtering residue load micropore filter bag in, put Enter program pressurized liquid extraction instrument, and add the ethanol solution accounting for mass ratio 1:20~50, be pressurized multiple solvent extraction through program, Collect filtrate;
3) merge all filtrates, precipitate with ethanol after cryoconcentration, sucking filtration, by filtrate through vacuum lyophilization, obtain in black currant fruit Anthocyan complex.
2. in black currant fruit as claimed in claim 1 anthocyan complex preparation method it is characterised in that: step 1) Described in beta-glucosidase be food stage, enzyme activity 100u~10000u/g.
3. in black currant fruit as claimed in claim 1 anthocyan complex preparation method it is characterised in that: step 2) Described in ethanol be food stage, 95% ethanol.
4. in black currant fruit as claimed in claim 1 anthocyan complex preparation method it is characterised in that: described step Rapid 2) Program supercharging solvent extraction condition is: 25 DEG C of temperature, air source pressure is 6bar, 18 DEG C of extraction temperature, and pressure is adjusted For every 2 minutes boosting 0.1bar, rise to 8bar and keep 2 hours, circulate 2 times.
5. the application of the black currant fruit anthocyan complex of any one of a kind of claim 1-4 preparation, its feature exists In: described black currant fruit anthocyan complex in mass ratio 0.005% is added in tobacco shred, coordinates with flue gas, enrich Fragrance, slightly fruit are fragrant and sweet, reduce and stimulate and reduce miscellaneous QI, improve aesthetic quality.
CN201610929440.4A 2016-10-31 2016-10-31 Preparing method and application of black currant fruit anthocyanin compound Pending CN106367205A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107723092A (en) * 2017-11-29 2018-02-23 湖北中烟工业有限责任公司 A kind of preparation method and applications with fragrant and sweet fermentation angelica extract

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101999648A (en) * 2010-10-11 2011-04-06 宝鸡市虹源生物科技有限公司 Extraction method of anthocyanin and original anthocyanin components in cranberry fruits
CN104489916A (en) * 2014-12-02 2015-04-08 云南中烟工业有限责任公司 Currant extract as well as preparation method and application thereof to cigarette

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101999648A (en) * 2010-10-11 2011-04-06 宝鸡市虹源生物科技有限公司 Extraction method of anthocyanin and original anthocyanin components in cranberry fruits
CN104489916A (en) * 2014-12-02 2015-04-08 云南中烟工业有限责任公司 Currant extract as well as preparation method and application thereof to cigarette

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107723092A (en) * 2017-11-29 2018-02-23 湖北中烟工业有限责任公司 A kind of preparation method and applications with fragrant and sweet fermentation angelica extract

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Application publication date: 20170201