CN113017140A - Method for preparing endogenous total enzyme by using fresh tobacco and application of endogenous total enzyme - Google Patents
Method for preparing endogenous total enzyme by using fresh tobacco and application of endogenous total enzyme Download PDFInfo
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- CN113017140A CN113017140A CN202110270580.6A CN202110270580A CN113017140A CN 113017140 A CN113017140 A CN 113017140A CN 202110270580 A CN202110270580 A CN 202110270580A CN 113017140 A CN113017140 A CN 113017140A
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- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 109
- 241000208125 Nicotiana Species 0.000 title claims abstract description 108
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 83
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 34
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 21
- 239000002994 raw material Substances 0.000 claims abstract description 20
- 239000006228 supernatant Substances 0.000 claims abstract description 20
- 239000002699 waste material Substances 0.000 claims abstract description 20
- 230000008569 process Effects 0.000 claims abstract description 16
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 239000012535 impurity Substances 0.000 claims abstract description 14
- 238000007710 freezing Methods 0.000 claims abstract description 11
- 230000008014 freezing Effects 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims abstract description 11
- 239000000047 product Substances 0.000 claims abstract description 10
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 9
- 239000011259 mixed solution Substances 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims abstract description 7
- 239000002245 particle Substances 0.000 claims abstract description 7
- 239000002244 precipitate Substances 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 3
- 238000007670 refining Methods 0.000 claims description 3
- 230000006872 improvement Effects 0.000 abstract description 5
- 238000002360 preparation method Methods 0.000 abstract description 4
- 239000000779 smoke Substances 0.000 abstract description 4
- 235000019505 tobacco product Nutrition 0.000 abstract description 2
- 229940088598 enzyme Drugs 0.000 description 61
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 102000030523 Catechol oxidase Human genes 0.000 description 3
- 108010031396 Catechol oxidase Proteins 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000003205 fragrance Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 101710184309 Probable sucrose-6-phosphate hydrolase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 102400000472 Sucrase Human genes 0.000 description 1
- 101710112652 Sucrose-6-phosphate hydrolase Proteins 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 238000012870 ammonium sulfate precipitation Methods 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/305—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances of undetermined constitution characterised by their preparation
- A24B15/307—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances of undetermined constitution characterised by their preparation using microorganisms or enzymes as catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Manufacture Of Tobacco Products (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The method for preparing the endogenous total enzyme by using fresh tobacco and the application of the endogenous total enzyme are characterized in that waste fresh tobacco raw materials are cleaned, squeezed to obtain juice, filtered to remove suspended plant particles, centrifuged to remove impurities, collected supernatant is subjected to ultrafiltration concentration, the concentrated solution is cooled to 0-4 ℃, ethanol is added while stirring until the mass concentration of the ethanol in the mixed solution is 10-90%, the mixed solution is stood, the supernatant is removed, and precipitates are collected to obtain the wet enzyme; and (3) freezing the wet enzyme at a low temperature of below 0 ℃, drying, and crushing to obtain the tobacco endogenous total enzyme product. The endogenous total enzyme is used as a tobacco leaf upgrading agent to be applied to the feeding process of a cigarette tobacco making line, or used as a tobacco leaf alcoholizing agent to be applied to the two-moistening link or the export feeding process of a threshing and redrying line. The tobacco endogenous biological enzyme product prepared by the invention has good quality and the preparation method is simple. The endogenous total enzyme is applied to quality improvement and alcoholization of tobacco leaves, can improve the smoke quality of tobacco products, and can also shorten the alcoholization time of the tobacco leaves.
Description
Technical Field
The invention relates to the technical field of preparation of tobacco additives, in particular to a method for preparing endogenous total enzymes by using waste fresh tobacco raw materials and application of the endogenous total enzymes in quality improvement and alcoholization of tobacco leaves.
Background
China is the largest tobacco producing country and consuming country in the world, wherein the yield of tobacco leaves in Yunnan province accounts for about 40 percent of the whole country. Due to the high standard of tobacco leaves for flue-cured tobacco, about 100 ten thousand tons of waste fresh tobacco raw materials are generated in tobacco fields after the tobacco leaves for flue-cured tobacco are harvested every year. The abandoned fresh tobacco raw materials mainly refer to top leaves and bottom leaves generated in the flue-cured tobacco planting process, fireworks, tobacco stems and tobacco stems, and fresh tobacco leaves which need to be treated in the field and have poor development, rigid structure and low industrial availability. Most of the waste fresh tobacco raw materials are burned or randomly buried, which not only pollutes the environment, but also causes the waste of precious resources and is easy to cause the diseases and insect pests of the tobacco fields. Therefore, the development and utilization of waste fresh tobacco raw materials are hot spots of research.
The waste fresh tobacco raw material contains a plurality of biological enzymes, and the endogenous total enzymes mainly comprise 5 biological enzymes: protease, amylase, cellulase, sucrase and polyphenol oxidase. Research shows that the alcoholization time can be shortened, the tobacco quality can be improved and the smoke characteristics can be improved by using microorganisms or enzymes for tobacco leaf fermentation, but generally, exogenous biological enzymes are applied, so that the dosage and the variety are difficult to select, and miscellaneous gas except the fragrance of the tobacco can be generated. The tobacco leaves subjected to the threshing and redrying process have low content of endogenous enzymes, and are not enough to fully convert starch, protein, cellulose, pectin and the like in the tobacco leaves in a short time, so that the internal chemical components of the tobacco leaves tend to be balanced, and the quality is improved. If the natural fermentation time is too long, the method occupies a factory and influences the capital turnover of a tobacco plant. Therefore, by utilizing proper technical means such as extraction and separation, endogenous total enzymes of waste fresh tobacco raw materials are developed, and the method has good market prospect for improving the quality of tobacco leaves and promoting alcoholization.
The published documents on the development and application of endogenous total enzymes of waste fresh tobacco raw materials are very rare, and part of related patents are basically limited to single biological enzymes and traditional complex processes. For example, in the separation technology of tobacco polyphenol oxidase disclosed in chinese patent CN102477413A, acetone precipitation and ammonium sulfate precipitation are used to prepare polyphenol oxidase, acetone has high toxicity and is not suitable for use in cigarettes, and ammonium sulfate requires a salting-out process which is too complicated and is not suitable for industrial production. A tobacco additive from fresh tobacco leaf and its preparation method and application are disclosed in Chinese patent CN105795505A, which is prepared by taking out main vein from mature middle tobacco leaf, adding phosphoric acid buffer solution and preparing with hollow fiber column for reducing cigarette tar. However, the mature middle tobacco leaves are tobacco leaves for flue-cured tobacco, are high in price, complicated in operation of removing the main veins, complex in product components, contain a plurality of pigments and the like, and external impurities are introduced into the phosphoric acid buffer solution, so that the method belongs to the application direction of harm reduction and tar reduction. The technical scheme adopted by the patents is not suitable for the development of endogenous total enzymes of waste fresh tobacco raw materials. At present, no report is found about a technology which integrates a low-temperature extraction and separation process by taking waste fresh tobacco as a raw material, develops tobacco endogenous total enzymes and is applied to the aspects of quality improvement and alcoholization of tobacco leaves.
Disclosure of Invention
The invention aims to fill the blank of the prior art and provides a method for preparing endogenous total enzyme by taking waste fresh tobacco as a raw material and integrating a low-temperature extraction and separation process. The invention also provides application of endogenous total enzymes in quality improvement and alcoholization of tobacco leaves.
The technical scheme adopted by the invention is as follows:
a method for preparing endogenous total enzymes by using fresh tobacco comprises the following steps:
(1) raw material treatment: cleaning the waste fresh tobacco raw material, squeezing to obtain juice, filtering with gauze to remove suspended plant particles, centrifuging to remove impurities, and collecting supernatant for later use;
(2) and (3) ultrafiltration concentration: concentrating the supernatant in ultrafiltration equipment to obtain a concentrated solution with a volume of 19-21% of the original volume;
(3) and (3) precipitation and refining: cooling the concentrated solution to 0-4 deg.C, adding ethanol under stirring until the mass concentration of ethanol in the mixed solution is 10-90%, standing, removing the supernatant, and collecting precipitate to obtain wet enzyme;
(4) and (3) freeze drying: and (3) freezing the wet enzyme at a low temperature of below 0 ℃, drying, and crushing to obtain the tobacco endogenous total enzyme product.
Further, the step (1) of centrifuging to remove impurities is to centrifuge at 3000-.
Further, the ultrafiltration concentration in the step (2) is carried out, wherein the cut-off molecular weight of an ultrafiltration membrane of the ultrafiltration equipment is 10-50kD, the ultrafiltration temperature is 4-35 ℃, and the ultrafiltration pressure is 0.10-0.40 MPa.
Further, the freezing time of freezing the wet enzyme at a low temperature of 0 ℃ or lower in the step (4) is 2 to 24 hours.
The application of the endogenous total enzyme prepared by the invention is to apply the endogenous total enzyme as a tobacco leaf upgrading agent to the feeding process of the cigarette tobacco-making thread, wherein the addition amount is 0.1-2.0% of the mass of tobacco leaves.
Another application of the endogenous total enzyme prepared by the invention is to apply the endogenous total enzyme as a tobacco mellowing agent to a two-moistening link or an export feeding process of a threshing and redrying line, wherein the addition amount is 0.1-2.0% of the mass of tobacco leaves.
The invention has the following advantages:
(1) the method for preparing the endogenous total enzyme by using the waste fresh tobacco raw materials is to prepare the tobacco endogenous biological enzyme by adopting a low-temperature technology, has the characteristics of no phase change, less energy consumption, less activity loss, simple operation and the like, and the prepared endogenous total enzyme product has good quality;
(2) the preparation method is simple, the process is reasonable, the used equipment is mature, and the industrial production is easy to realize;
(3) the endogenous total enzyme is completely from the tobacco itself, and does not introduce exogenous impurities and bring exogenous miscellaneous gas. The endogenous total enzyme is applied to quality improvement and alcoholization of tobacco leaves, so that the tobacco fragrance can be increased, the impurity removal function of biological enzyme can be exerted, and exogenous impurities and exogenous miscellaneous gas cannot be introduced, thereby improving the smoke quality of tobacco products.
(3) The alcoholization time of the tobacco leaves can be shortened, and the tobacco leaf alcoholization is promoted.
(4) The waste fresh tobacco raw material is developed and utilized, and the economic benefit and the social benefit are better.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail by the following embodiments. The specific embodiments described are merely illustrative of the invention and do not limit the invention.
Example 1
A method for preparing endogenous total enzymes by using fresh tobacco comprises the following steps:
(1) raw material treatment: cleaning waste fresh tobacco raw materials, squeezing juice with a squeezer, filtering with gauze to remove suspended plant particles, centrifuging at 3000r/min for 60min with a centrifuge to remove impurities, and collecting supernatant for later use;
(2) and (3) ultrafiltration concentration: concentrating the supernatant in ultrafiltration equipment, wherein the ultrafiltration membrane has a molecular weight cut-off (MWCO) of 10kD, a temperature of 4 ℃ and a pressure of 0.10MPa, so that the volume of the liquid is concentrated to about 20% of the original volume;
(3) and (3) precipitation and refining: cooling the concentrated solution to 0-2 deg.C, adding 95% edible ethanol under stirring until the ethanol concentration in the mixed solution is 10%, standing to precipitate enzyme, removing supernatant, and collecting precipitate to obtain wet enzyme;
(4) and (3) freeze drying: and (3) freezing the wet enzyme at a low temperature of below 0 ℃ for 2h, drying, and crushing to obtain the tobacco endogenous total enzyme product.
The prepared endogenous total enzyme is used as a tobacco leaf upgrading agent and applied to the feeding process of the cigarette tobacco-making line, the addition amount is 0.1 percent of the mass of the tobacco leaves, the tobacco fragrance can be increased, the biological enzyme impurity removal function can be exerted, and the smoke quality of the finally obtained tobacco shreds is improved.
The prepared endogenous total enzyme can also be used as a tobacco mellowing agent to be applied to the two-moistening link of the threshing and redrying line, the addition amount is 0.1 percent of the mass of the tobacco, the existing threshing and redrying line equipment is not required to be changed, the mellowing time is shortened, and the mellowing of the tobacco is promoted.
Example 2
A method for preparing endogenous total enzyme from fresh tobacco comprises cleaning waste fresh tobacco, squeezing to obtain juice, filtering with gauze to remove suspended plant particles, centrifuging at 15000r/min for 10min to remove impurities, and collecting supernatant. Concentrating the supernatant in ultrafiltration equipment, wherein the cut-off Molecular Weight (MWCO) of the ultrafiltration membrane is 50kD, the temperature is 35 ℃, and the pressure is 0.40MPa, so that the volume of the liquid is concentrated to 19% of the original volume. Cooling the concentrated solution to 2-4 deg.C, adding edible ethanol under stirring until the mass concentration of ethanol in the mixed solution is 90%, standing to precipitate enzyme, removing supernatant, and collecting precipitate to obtain wet enzyme. And (3) freezing the wet enzyme at a low temperature of below 0 ℃ for 24h, drying, and crushing to obtain the tobacco endogenous total enzyme product.
The prepared endogenous total enzyme is used as a tobacco leaf upgrading agent and applied to the feeding process of the cigarette tobacco-making line, and the adding amount is 2% of the mass of tobacco leaves.
The prepared endogenous total enzyme can also be used as a tobacco mellowing agent and applied to the outlet feeding process of the threshing and redrying line, and the adding amount is 1.5 percent of the mass of the tobacco.
Example 3
A method for preparing endogenous total enzyme from fresh tobacco comprises cleaning waste fresh tobacco, squeezing to obtain juice, filtering with gauze to remove suspended plant particles, centrifuging at 4000r/min for 30min to remove impurities, and collecting supernatant. Concentrating the supernatant in ultrafiltration equipment with MWCO of 20kD, temperature of 25 deg.C, and pressure of 0.20MPa to obtain a concentrated solution with volume of 21% of the original volume. Cooling the concentrated solution to 3 ℃, adding ethanol while stirring until the mass concentration of the ethanol in the mixed solution is 20%, standing to precipitate the enzyme, removing the supernatant, and collecting the precipitate to obtain the wet enzyme. And (3) freezing the wet enzyme at a low temperature of below 0 ℃ for 4h, drying, and crushing to obtain the tobacco endogenous total enzyme product.
The prepared endogenous total enzyme is used as a tobacco leaf upgrading agent and applied to the feeding process of the cigarette tobacco-making line, and the adding amount is 1.5 percent of the mass of tobacco leaves.
The prepared endogenous total enzyme can also be used as a tobacco mellowing agent to be applied to the two-moistening link of the threshing and redrying line, and the addition amount is 2 percent of the mass of the tobacco.
Example 4
A method for preparing endogenous total enzyme from fresh tobacco comprises cleaning waste fresh tobacco, squeezing to obtain juice, filtering with gauze to remove suspended plant particles, centrifuging at 8000r/min for 20min to remove impurities, and collecting supernatant. Concentrating the supernatant in ultrafiltration equipment with MWCO of 30kD, temperature of 30 deg.C, and pressure of 0.30MPa to concentrate the liquid volume to about 20% of the original volume. Cooling the concentrated solution to 4 ℃, adding edible ethanol with the mass concentration of 95% while stirring until the mass concentration of the ethanol in the mixed solution is 60%, standing to precipitate the enzyme, removing the supernatant, and collecting the precipitate to obtain the wet enzyme. And (3) freezing the wet enzyme at a low temperature of below 0 ℃ for 12h, drying, and crushing to obtain the tobacco endogenous total enzyme product.
The prepared endogenous total enzyme is used as a tobacco leaf upgrading agent and applied to the feeding process of the cigarette tobacco-making line, and the adding amount is 2% of the mass of tobacco leaves.
The prepared endogenous total enzyme can also be used as a tobacco mellowing agent to be applied to the two-moistening link of the threshing and redrying line, and the addition amount is 2 percent of the mass of the tobacco.
Claims (6)
1. The method for preparing endogenous total enzymes by using fresh tobacco is characterized by comprising the following steps:
(1) raw material treatment: cleaning the waste fresh tobacco raw material, squeezing to obtain juice, filtering with gauze to remove suspended plant particles, centrifuging to remove impurities, and collecting supernatant for later use;
(2) and (3) ultrafiltration concentration: concentrating the supernatant in ultrafiltration equipment to obtain a concentrated solution with a volume of 19-21% of the original volume;
(3) and (3) precipitation and refining: cooling the concentrated solution to 0-4 deg.C, adding ethanol under stirring until the mass concentration of ethanol in the mixed solution is 10-90%, standing, removing the supernatant, and collecting precipitate to obtain wet enzyme;
(4) and (3) freeze drying: and (3) freezing the wet enzyme at a low temperature of below 0 ℃, drying, and crushing to obtain the tobacco endogenous total enzyme product.
2. The method for preparing endogenous total enzymes according to claim 1, wherein the step (1) of centrifuging to remove impurities is performed at 3000-15000r/min for 10-60min by using a centrifuge.
3. The method for preparing endogenous total enzymes from fresh tobacco according to claim 1 or 2, wherein the ultrafiltration concentration in step (2) is performed by ultrafiltration membrane with cut-off molecular weight of 10-50kD, ultrafiltration temperature of 4-35 deg.C, and ultrafiltration pressure of 0.10-0.40 MPa.
4. The method for preparing endogenous total enzymes from fresh tobacco according to claim 1 or 2, wherein the freezing time for freezing the wet enzymes at a temperature of 0 ℃ or lower in the step (4) is 2 to 24 hours.
5. The application of the endogenous total enzyme of any one of claims 1 to 4, which is to apply the endogenous total enzyme as a tobacco leaf quality-improving agent to the feeding process of the cigarette tobacco-making line, wherein the addition amount is 0.1 to 2.0 percent of the mass of tobacco leaves.
6. The application of the endogenous total enzyme of any one of claims 1 to 4, which is to apply the endogenous total enzyme as a tobacco mellowing agent to a second moistening link or an export feeding process of a threshing and redrying line, wherein the addition amount is 0.1 to 2.0 percent of the mass of tobacco leaves.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110270580.6A CN113017140A (en) | 2021-03-12 | 2021-03-12 | Method for preparing endogenous total enzyme by using fresh tobacco and application of endogenous total enzyme |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110270580.6A CN113017140A (en) | 2021-03-12 | 2021-03-12 | Method for preparing endogenous total enzyme by using fresh tobacco and application of endogenous total enzyme |
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| CN202110270580.6A Pending CN113017140A (en) | 2021-03-12 | 2021-03-12 | Method for preparing endogenous total enzyme by using fresh tobacco and application of endogenous total enzyme |
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2021
- 2021-03-12 CN CN202110270580.6A patent/CN113017140A/en active Pending
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Application publication date: 20210625 |