CN105784705A - Forensic medicine diatom test method - Google Patents
Forensic medicine diatom test method Download PDFInfo
- Publication number
- CN105784705A CN105784705A CN201610099515.0A CN201610099515A CN105784705A CN 105784705 A CN105784705 A CN 105784705A CN 201610099515 A CN201610099515 A CN 201610099515A CN 105784705 A CN105784705 A CN 105784705A
- Authority
- CN
- China
- Prior art keywords
- diatom
- hydrogel
- test method
- sulfadimidine
- forensic medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a forensic medicine diatom test method.The forensic medicine diatom test method comprises the following steps that sulfamethazine, acrylic acid, acrylamide, N'N-methylene bisacrylamide, ammonium persulfate and deionized water are taken according to a proportion and fully mixed in a beaker; after oxygen is removed with nitrogen, the mixture is poured into a sandwich space formed by two glass slides and then put into an oven at the temperature of 60 DEG C for a polymerization reaction for 3 h, and film-state imprinting hydrogel is prepared; the obtained imprinting hydrogel is soaked in a 10% acetic acid solution, template molecules sulfadimidine in the imprinting hydrogel are removed through ultrasonic oscillation, then the imprinting hydrogel is soaked in a sample to be tested for 5-10 min and then taken out, and then the imprinting hydrogel is put under an optical microscope for observation; diatom on the filter film or a shot picture is checked, classified and counted in a manual recognition mode or an automatic computer recognition mode.The forensic medicine diatom test method is high in yield, efficiency and performance cost ratio, easy to popularize, accurate in qualitative and quantitative analysis and low in labor intensity.
Description
Technical field
The present invention relates to and algae inspection field, be specifically related to a kind of diatom inspection method in medical jurisprudence.
Background technology
Diatom is a kind of unicellular alga, and the whole world there are about kind more than 16000, and body length is typically in 1 μm to 200
Between μm, its distinct feature is that most cell wall in addition to individual species, the most siliceousization, in formation,
Lower two transparent shells, form a siliceous cell wall with girdle band fit.The living environment of diatom is the widest
General, in nature waters, almost can see the trace of diatom.
Type common during corpse is Medicolegal examination in water.Since the forties in 20th century, many methods
Doctor scholar confirms on drowned person's corpse, and diatom can be entered multiple internal organs by blood circulation.Frustule wall because of
Resistance is strong and is difficult to be destroyed, siliceous content height person, uses concentrated sulphuric acid, concentrated nitric acid to boil even high temperature and burns
Burn and be not destroyed.Therefore, diatom examination is to solve a kind of reliable means of the cause of the death problem of corpse in water.
In medicolegal practice, 1-30g histoorgan would generally leave the digestion solution of 10-50ml after clearing up,
Centrifugal is the common method of diatom in enrichment water sample or histoorgan digestion solution.As GA/T 813-2008 marks
In accurate (" in human tissue organ, diatom nitric acid breaks the inspection of machine method "), postdigestive digestion solution is poured into from
Heart Guan Jing is centrifuged repeatedly rear film-making；Finally use om observation method, check diatom with 10 times or 40 times of object lens.
Show according to research reports such as Hu Sun Lin, be centrifuged and once cause the diatom of about 10% to lose, increase centrifugal speed
Degree can not significantly improve the response rate of diatom, therefore causes the diatom response rate in organs and tissues low.
Owing to the method for vacuum filtration also can be enriched with diatom, but filter membrane itself is opaque, it is impossible to directly
Being placed in optical microphotograph Microscopic observation, therefore Hu Sun Lin etc. attempt using vacuum filtration-automatic scanning Electronic Speculum
Method observes diatom.Although the resolution of ultramicroscope is high, it is clear to observe, but, in view of its operation is multiple
Feature miscellaneous, relatively costly, that maintenance cost is high so that ultramicroscope can only be at some big-and-middle-sized laboratorys
Use with scientific research institution, it is impossible to be widely used in grass-roots method medical laboratory.
Summary of the invention
For solving the problems referred to above, the invention provides a kind of diatom inspection method in medical jurisprudence, check highly sensitive,
Checkability is high, will not dramatically increase equipment cost again, also has eco-friendly advantage, and can meet existing
Field, the demand of high throughput testing.
For achieving the above object, the technical scheme that the present invention takes is:
A kind of diatom inspection method in medical jurisprudence, comprises the steps:
S1, take 0.15-0.19 part sulfadimidine, 12-16 part acrylic acid, 25-35 part propylene respectively
Amide, 0.8-1 part N ' N-methylene-bisacrylamide, 0.8-1 part Ammonium persulfate. and 35-45 part go from
Sub-water is sufficiently mixed in beaker, after nitrogen deoxygenation, fills it into the clamping plate formed by two panels microscope slide
In space, it is placed in polyreaction 3h in the baking oven of 60 DEG C, obtains the trace hydrogel of film like；
S2, the trace hydrogel of step S1 gained is immersed in the acetum of 10%, and sonic oscillation 5
-10min, to remove template molecule sulfadimidine therein；
S3, the trace hydrogel of step S3 gained is immersed in detected sample and takes out after 5-10min, put
In optical microphotograph Microscopic observation；Use artificial cognition mode or computer automation recognition method on filter membrane
Diatom or captured picture carry out checking, classifying and statistical disposition.
Preferably, described sample to be tested is that water sample, tracheal strips are drowned the digestion solution of liquid or histoorgan.
The method have the advantages that
1) response rate is high, use special hydrogel enrichment diatom, it is to avoid many in conventional centrifugal concentration method
The loss of secondary the caused diatom of centrifugal treating process, the hydrogel used is transparent, and light can be the most saturating
Filter membrane is to ensure om observation.Observation by light microscope result shows, uses hydrogel to have no effect on it fixed
Amount.Therefore, compared with traditional centrifugal enrichment method, use filter membrane concentration method from water sample and tissue digestion solution
Enrichment diatom, operates the easiest, and the response rate is higher.
2), efficiency is high, cost performance is high, easy to spread.The preparation of hydrogel, the enriching step behaviour of diatom
Make easy, take a short time, every kind of sample only need to be enriched with once, film-making one, it is to avoid conventional centrifugal is rich
Repeatedly being centrifuged repeatedly of collection method, repeatedly smear, film-making, both wasted time and energy, and makes again the response rate reduce.Film is saturating
Brightization step is simple, and the time is short, it is highly suitable for, and funds are few, small scale but to pitch widely distributed basic unit real
Test room.
3) qualitative and quantitative analysis is accurate, labor intensity is low.Special hydrogel concentration method film-making is used to improve
The diatom response rate, film-making amount reduces, it is not necessary to too much training and operation, and can in conjunction with computer automation
Captured picture is checked, classifies and statistical disposition by recognition method, and these can significantly reduce analysis
The labor intensity of personnel.
Detailed description of the invention
In order to make objects and advantages of the present invention clearer, below in conjunction with embodiment, the present invention is carried out
Further describe.Should be appreciated that specific embodiment described herein only in order to explain the present invention,
It is not intended to limit the present invention.
Embodiment 1
S1, respectively take 0.15 part of sulfadimidine, 12 parts of acrylic acid, 25 parts of acrylamides, 0.8 part
N ' N-methylene-bisacrylamide, 0.8 part of Ammonium persulfate. and 35 parts of deionized waters are sufficiently mixed in beaker,
After nitrogen deoxygenation, fill it in the clamping plate space formed by two panels microscope slide, be placed in the baking of 60 DEG C
Polyreaction 3h in case, obtains the trace hydrogel of film like；
S2, the trace hydrogel of step S1 gained is immersed in the acetum of 10%, and sonic oscillation 5
Min, to remove template molecule sulfadimidine therein；
S3, the trace hydrogel of step S3 gained is immersed in detected sample and takes out after 5min, be placed in light
Learn basis of microscopic observation；Use artificial cognition mode or computer automation recognition method to the diatom on filter membrane
Or captured picture carries out checking, classifying and statistical disposition；Wherein, described sample to be tested be water sample,
Tracheal strips is drowned the digestion solution of liquid or histoorgan.
Embodiment 2
S1, take 16 parts of acrylic acid of 0.19 part of sulfadimidine, 35 parts of acrylamides, 1 part of N ' respectively
N-methylene-bisacrylamide, 1 part of Ammonium persulfate. and 45 parts of deionized waters are sufficiently mixed in beaker, warp
After nitrogen deoxygenation, fill it in the clamping plate space formed by two panels microscope slide, be placed in the baking oven of 60 DEG C
Middle polyreaction 3h, obtains the trace hydrogel of film like；
S2, the trace hydrogel of step S1 gained is immersed in the acetum of 10%, and sonic oscillation
510min, to remove template molecule sulfadimidine therein；
S3, the trace hydrogel of step S3 gained is immersed in detected sample and takes out after 10min, be placed in light
Learn basis of microscopic observation；Use artificial cognition mode or computer automation recognition method to the diatom on filter membrane
Or captured picture carries out checking, classifying and statistical disposition；Wherein, described sample to be tested be water sample,
Tracheal strips is drowned the digestion solution of liquid or histoorgan.
Embodiment 3
S1, respectively take 0.17 part of sulfadimidine, 14 parts of acrylic acid, 30 parts of acrylamides, 0.9 part
N ' N-methylene-bisacrylamide, 0.9 part of Ammonium persulfate. and 40 parts of deionized waters are sufficiently mixed in beaker,
After nitrogen deoxygenation, fill it in the clamping plate space formed by two panels microscope slide, be placed in the baking of 60 DEG C
Polyreaction 3h in case, obtains the trace hydrogel of film like；
S2, the trace hydrogel of step S1 gained is immersed in the acetum of 10%, and sonic oscillation
7.5min, to remove template molecule sulfadimidine therein；
S3, the trace hydrogel of step S3 gained is immersed in detected sample and takes out after 7.5min, be placed in
Optical microphotograph Microscopic observation；Use artificial cognition mode or computer automation recognition method to the silicon on filter membrane
Algae or captured picture carry out checking, classifying and statistical disposition；Wherein, described sample to be tested be water sample,
Tracheal strips is drowned the digestion solution of liquid or histoorgan.
The above is only the preferred embodiment of the present invention, it is noted that common for the art
For technical staff, under the premise without departing from the principles of the invention, it is also possible to make some improvements and modifications,
These improvements and modifications also should be regarded as protection scope of the present invention.
Claims (2)
1. a diatom inspection method in medical jurisprudence, it is characterised in that comprise the steps:
S1, take 0.15-0.19 part sulfadimidine, 12-16 part acrylic acid, 25-35 part propylene respectively
Amide, 0.8-1 part N ' N-methylene-bisacrylamide, 0.8-1 part Ammonium persulfate. and 35-45 part go from
Sub-water is sufficiently mixed in beaker, after nitrogen deoxygenation, fills it into the clamping plate formed by two panels microscope slide
In space, it is placed in polyreaction 3h in the baking oven of 60 DEG C, obtains the trace hydrogel of film like；
S2, the trace hydrogel of step S1 gained is immersed in the acetum of 10%, and sonic oscillation 5
-10min, to remove template molecule sulfadimidine therein；
S3, the trace hydrogel of step S3 gained is immersed in detected sample and takes out after 5-10min, put
In optical microphotograph Microscopic observation；Use artificial cognition mode or computer automation recognition method on filter membrane
Diatom or captured picture carry out checking, classifying and statistical disposition.
A kind of diatom inspection method in medical jurisprudence the most according to claim 1, it is characterised in that described in treat
Sample survey is that water sample, tracheal strips are drowned the digestion solution of liquid or histoorgan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610099515.0A CN105784705B (en) | 2016-02-21 | 2016-02-21 | A kind of diatom inspection method in medical jurisprudence |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610099515.0A CN105784705B (en) | 2016-02-21 | 2016-02-21 | A kind of diatom inspection method in medical jurisprudence |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105784705A true CN105784705A (en) | 2016-07-20 |
| CN105784705B CN105784705B (en) | 2018-05-18 |
Family
ID=56403594
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610099515.0A Expired - Fee Related CN105784705B (en) | 2016-02-21 | 2016-02-21 | A kind of diatom inspection method in medical jurisprudence |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105784705B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345246A (en) * | 2017-06-16 | 2017-11-14 | 广州市刑事科学技术研究所 | A kind of diatom rbcL genetic analysis method and its application in legal medical expert detects |
| CN108844802A (en) * | 2018-06-25 | 2018-11-20 | 孙鑫 | A kind of diatom fluorescent staining liquid and the methods for detecting diatom for detecting organs and tissues |
| CN110033445A (en) * | 2019-04-10 | 2019-07-19 | 司法鉴定科学研究院 | Medicolegal examination automatic identification system and recognition methods based on deep learning |
| CN110042141A (en) * | 2019-05-17 | 2019-07-23 | 广州竞赢科学仪器有限公司 | A kind of diatom inspection method in medical jurisprudence based on Flow Optical micro-imaging |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2131699C1 (en) * | 1995-01-11 | 1999-06-20 | Научно-исследовательский институт биологии при Иркутском государственном университете | Method of detection of diatoms in sunk human |
| CN102507585A (en) * | 2011-11-11 | 2012-06-20 | 广州市刑事科学技术研究所 | Forensic medical diatom inspection method based on mixed acid digestion |
| WO2014014894A2 (en) * | 2012-07-18 | 2014-01-23 | Auburn University | Self-assembled nanostructured sensors and methods thereof |
| CN103674980A (en) * | 2013-12-03 | 2014-03-26 | 上海师范大学 | Microscopic observation method of diatom |
| CN103776835A (en) * | 2014-01-27 | 2014-05-07 | 广州市刑事科学技术研究所 | Forensic medicine diatom detection method based on filter membrane |
-
2016
- 2016-02-21 CN CN201610099515.0A patent/CN105784705B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2131699C1 (en) * | 1995-01-11 | 1999-06-20 | Научно-исследовательский институт биологии при Иркутском государственном университете | Method of detection of diatoms in sunk human |
| CN102507585A (en) * | 2011-11-11 | 2012-06-20 | 广州市刑事科学技术研究所 | Forensic medical diatom inspection method based on mixed acid digestion |
| WO2014014894A2 (en) * | 2012-07-18 | 2014-01-23 | Auburn University | Self-assembled nanostructured sensors and methods thereof |
| CN103674980A (en) * | 2013-12-03 | 2014-03-26 | 上海师范大学 | Microscopic observation method of diatom |
| CN103776835A (en) * | 2014-01-27 | 2014-05-07 | 广州市刑事科学技术研究所 | Forensic medicine diatom detection method based on filter membrane |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345246A (en) * | 2017-06-16 | 2017-11-14 | 广州市刑事科学技术研究所 | A kind of diatom rbcL genetic analysis method and its application in legal medical expert detects |
| CN107345246B (en) * | 2017-06-16 | 2020-09-29 | 广州市刑事科学技术研究所 | Diatom rbcL gene analysis method and application thereof in forensic detection |
| CN108844802A (en) * | 2018-06-25 | 2018-11-20 | 孙鑫 | A kind of diatom fluorescent staining liquid and the methods for detecting diatom for detecting organs and tissues |
| CN108844802B (en) * | 2018-06-25 | 2020-09-22 | 孙鑫 | Diatom fluorescence staining solution and diatom detection method for detecting visceral organs |
| CN110033445A (en) * | 2019-04-10 | 2019-07-19 | 司法鉴定科学研究院 | Medicolegal examination automatic identification system and recognition methods based on deep learning |
| CN110042141A (en) * | 2019-05-17 | 2019-07-23 | 广州竞赢科学仪器有限公司 | A kind of diatom inspection method in medical jurisprudence based on Flow Optical micro-imaging |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105784705B (en) | 2018-05-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105784705A (en) | Forensic medicine diatom test method | |
| Koch | Investigations into the etiology of traumatic infective diseases | |
| EP1152053A3 (en) | Method and apparatus for the preparation of a vascularized bioartificial tissue | |
| CN102620966B (en) | Biological sample preparation method applicable to ultra-thin section and fluorescence imaging | |
| CN104483178B (en) | The preparation method of epinephelus akaara adult fish chromosome | |
| CN107183013A (en) | A kind of method of donkey testicular fluid Cord blood | |
| Matsumoto et al. | A simple method for diatom detection in drowning | |
| CN103776835B (en) | A kind of diatom inspection method in medical jurisprudence based on filter membrane | |
| Umbreit et al. | Analysis of plant tissue | |
| Qasimov | SPREAD OF DISEASES OF METABOLISM DISORDERS IN FISH | |
| CN105067602A (en) | Copper ion test paper and method for testing copper ions in water | |
| CN104597016B (en) | It is inverted laser confocal microscope animals and plants vivo observation device and method | |
| AT4098U1 (en) | QUICK TEST FOR DETERMINING THE FORMALDEHYDE | |
| Wyckoff | Ultraviolet microscopy as a means of studying cell structure | |
| CN204359693U (en) | Be inverted laser confocal microscope animals and plants vivo observation device | |
| CN117147250B (en) | Tooth transparentizing method for small mammals for experiments | |
| CN110093308A (en) | A kind of attached cell method for resuscitation | |
| CN113018229B (en) | Application and preparation method of carrot seed extracting solution and firming water | |
| RU2605843C2 (en) | Method for microscopic evaluation of aggregate state of erythrocytes in autoplasma | |
| UA143815U (en) | METHOD OF DETERMINING THE LIMITATION OF DEATH OF DEATH BY THE METHOD OF SPECTRAL-SELECTIVE LASER FLUORESCENT MICROSCOPY OF LAYERS OF THE GLASSY BODY | |
| CN106353160A (en) | Protecting method for fluorescence signal of fluorescent protein in tissue sample and dehydration composition | |
| Staine et al. | Automated identification of rare sperm becomes possible: Is sperm selection the next frontier in male infertility? | |
| CN106568931A (en) | Method for detecting cardiotoxicity of nano-material | |
| Shckorbatov et al. | Changes in chromatin state in donors subjected to physical stress | |
| Baldwin | Effects Produced by X-Ray Energy Acting Upon Frogs' Ova in Early Developmental Stages |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180518 Termination date: 20190221 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |