CN103776835B - A kind of diatom inspection method in medical jurisprudence based on filter membrane - Google Patents
A kind of diatom inspection method in medical jurisprudence based on filter membrane Download PDFInfo
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- CN103776835B CN103776835B CN201410039415.XA CN201410039415A CN103776835B CN 103776835 B CN103776835 B CN 103776835B CN 201410039415 A CN201410039415 A CN 201410039415A CN 103776835 B CN103776835 B CN 103776835B
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- 239000012528 membrane Substances 0.000 title claims abstract description 103
- 238000000034 method Methods 0.000 title claims abstract description 50
- 238000007689 inspection Methods 0.000 title claims abstract description 20
- 238000003828 vacuum filtration Methods 0.000 claims abstract description 16
- 238000000879 optical micrograph Methods 0.000 claims abstract description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 45
- 238000000967 suction filtration Methods 0.000 claims description 36
- 239000000243 solution Substances 0.000 claims description 29
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 claims description 26
- 239000003153 chemical reaction reagent Substances 0.000 claims description 25
- 239000005770 Eugenol Substances 0.000 claims description 15
- 229960002217 eugenol Drugs 0.000 claims description 15
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 claims description 13
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 claims description 13
- AHJKWBGXAGSAIK-UHFFFAOYSA-N acetic acid;2-methoxy-4-prop-2-enylphenol Chemical compound CC(O)=O.COC1=CC(CC=C)=CC=C1O AHJKWBGXAGSAIK-UHFFFAOYSA-N 0.000 claims description 13
- 230000019771 cognition Effects 0.000 claims description 12
- 230000029087 digestion Effects 0.000 claims description 11
- 238000004451 qualitative analysis Methods 0.000 claims description 9
- 238000004445 quantitative analysis Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 210000000988 bone and bone Anatomy 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 230000007935 neutral effect Effects 0.000 claims description 7
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 7
- 239000012498 ultrapure water Substances 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 239000004695 Polyether sulfone Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 229920006393 polyether sulfone Polymers 0.000 claims description 2
- 238000004017 vitrification Methods 0.000 abstract description 11
- 241000195493 Cryptophyta Species 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 abstract description 3
- 210000001519 tissue Anatomy 0.000 description 13
- 210000004072 lung Anatomy 0.000 description 9
- 230000003287 optical effect Effects 0.000 description 7
- 238000011084 recovery Methods 0.000 description 6
- 229920012266 Poly(ether sulfone) PES Polymers 0.000 description 5
- 239000006059 cover glass Substances 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 238000012797 qualification Methods 0.000 description 5
- 230000000007 visual effect Effects 0.000 description 5
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
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- 238000011160 research Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 238000011888 autopsy Methods 0.000 description 2
- 230000001684 chronic effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- HQFCOGRKGVGYBB-UHFFFAOYSA-N ethanol;nitric acid Chemical compound CCO.O[N+]([O-])=O HQFCOGRKGVGYBB-UHFFFAOYSA-N 0.000 description 2
- 230000002440 hepatic effect Effects 0.000 description 2
- 210000005228 liver tissue Anatomy 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 210000005084 renal tissue Anatomy 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 206010010071 Coma Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000001117 sulphuric acid Substances 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
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Abstract
The present invention relates to algae inspection field, specifically disclose a kind of diatom inspection method in medical jurisprudence based on filter membrane.After filter membrane after vacuum filtration is carried out Vitrification management by the present invention, the filter membrane after transparence can be placed directly in optical microphotograph Microscopic observation.Detection method of the present invention is easy and simple to handle, checkability is high and do not need to rely on complicated instrument, just can detect, have more widespread use in grass-roots method medical laboratory.
Description
Technical field
The present invention relates to algae inspection field, be specifically related to a kind of diatom inspection method in medical jurisprudence based on filter membrane.
Background technology
Diatom is a kind of unicellular algae, and about there is kind more than 16000 in the whole world, and body is long general between 1 μm to 200 μm, its the most obvious feature is that cell membrane is except individual species, all height silicidizes, form upper and lower two transparent shells, form a siliceous cell wall with girdle band fit.Widely, the waters of occurring in nature, almost can see the trace of diatom to the living environment of diatom.
In water, corpse is type common in Medicolegal examination.Since the forties in 20th century, many legal medical expert scholars confirm on drowned person's corpse, and diatom can enter multiple internal organs by the circulation system.Frustule wall by force and is not easily destroyed because of resistibility, siliceous content height person, uses the concentrated sulphuric acid, red fuming nitric acid (RFNA) boils even high temperature and burn and be not also destroyed.Therefore, diatom examination is a kind of reliable means of the cause of the death problem solving corpse in water.
In medicolegal practice, 1 ~ 30g histoorgan clears up the rear digestion solution that usually can leave 10 ~ 50ml, and centrifugal is the common method of diatom in enrichment water sample or histoorgan digestion solution.As in GA/T813-2008 standard (" in human tissue organ diatom nitric acid break machine method inspection "), postdigestive digestion solution is poured into centrifuge tube film-making after repeatedly centrifugal; Finally adopt om observation method, check diatom with 10 times or 40 times of object lens.Show according to the research such as Hu Sun Lin report, centrifugally once cause the diatom of about 10% lose, increase centrifugal speed can not significantly improve the recovery of diatom, therefore causes the diatom recovery in organs and tissues low.
Because the method for vacuum filtration also can enrichment diatom, but filter membrane itself is opaque, cannot directly be placed in optical microphotograph Microscopic observation, and therefore Sun Lin etc. attempts using the method for vacuum filtration-automatic scanning Electronic Speculum to observe diatom recklessly.Although the resolution of electron microscope is high, it is clear to observe, but, in view of its complicated operation, cost is higher, maintenance cost is high feature, electron microscope can only be used in some big-and-middle-sized laboratories and scientific research institution, grass-roots method medical laboratory cannot be widely used in.
Summary of the invention
The technical problem to be solved in the present invention is the above-mentioned defect in order to overcome prior art, provide a kind of check highly sensitive, checkability is high, significantly can not increase again the diatom inspection method in medical jurisprudence based on filter membrane of equipment cost.
Object of the present invention is achieved by the following technical programs.
Based on a diatom inspection method in medical jurisprudence for filter membrane, comprise the steps:
S1. filter membrane enrichment: adopt Vacuum filtration device that check sample is carried out vacuum filtration; Add ultrapure water after sample suction filtration completes and continue suction filtration, make filter membrane surface close to neutral;
S2. film transparence: be placed on microslide by filter membrane drying, drips acetic acid-eugenol reagent on filter membrane, covered;
S3. qualitative and quantitative analysis: the filter membrane after transparent is placed in optical microphotograph Microscopic observation; Artificial cognition mode or computer automation recognition method is adopted to check the diatom on filter membrane or captured picture, classify and statistical treatment.
In prior art, after adopting filter membrane concentration method that testing sample is carried out suction filtration, because filter membrane itself is opaque, cannot directly be placed in optical microphotograph Microscopic observation, so by the facilities for observation of complexity as automatic scanning Electronic Speculum, must detect the diatom on filter membrane, the present invention is by suitable Vitrification management mode, filter membrane can be made transparent, optical microphotograph Microscopic observation diatom can be placed directly in.
The method of the invention is suitable for all types of detection sample, as water sample, tracheal strips are drowned the digestion solution of liquid or histoorgan.
First filter membrane will be carried out the drying process examined during filter membrane Vitrification management, preferably, the method for described filter membrane drying is: on the electric furnace that the filter membrane after being completed by suction filtration is placed in 50 ~ 80 DEG C or baking oven heating 1 ~ 10min make its bone dry.Filter membrane is carried out Vitrification management by acting as of the acetic acid used in filter membrane Vitrification management process-eugenol reagent.Inventor also tries to adopt acetic acid or eugenol reagent to carry out Vitrification management to filter membrane in previous research work, but effect is all not so good as to carry out the effective of Vitrification management with acetic acid-eugenol reagent to filter membrane.As preferred embodiment, described acetic acid-eugenol reagent is that the eugenol solution of the acetic acid solution of concentration 80 ~ 100% and concentration 60 ~ 100% is mixed by the ratio uniform of 1:9 ~ 9:1 the mixed solution obtained.
In filter membrane enrichment process, simply connected or multi-joint Vacuum filtration device can be adopted to carry out vacuum filtration, simply connected Vacuum filtration device, each is carried out suction filtration to a sample; Multi-joint Vacuum filtration device, can carry out suction filtration process to multiple sample at every turn simultaneously.Preferably, during vacuum filtration, suction filtration vacuum tightness is: 100 ~ 800mmHg, and described filter membrane is the filter membrane of polyethersulfone material, and filter membrane diameter is 10 ~ 50mm, aperture is 0.1 ~ 1.0 μm.
Compared with prior art, the present invention has following beneficial effect:
1, the recovery is high.Filter membrane enriching step of the present invention, to avoid in conventional centrifugal concentration method repeatedly centrifugal treating process cause the loss of diatom.The transparence reagent adopted can by complete for filter membrane transparence, light can in a large number through filter membrane to ensure om observation.Observation by light microscope result shows, the filter membrane after transparence does not affect that it is quantitative.Therefore, compared with traditional centrifugal enrichment method, use filter membrane concentration method from water sample and organize enrichment diatom digestion solution, operate more easy, the recovery is higher.
2, efficiency is high, cost performance is high, be easy to popularization.Filter membrane enriching step is easy and simple to handle, take a short time, and often kind of sample only needs enrichment once, film-making one, and avoid the repeatedly repeatedly centrifugal of conventional centrifugal concentration method, repeatedly smear, film-making, both wasted time and energy, and makes again the recovery reduce.Film transparence step is simple, and the time is short, and the sample after transparent can directly be placed in optical microphotograph Microscopic observation, can ensure the accuracy of assay, again without the need to extra procuring equipment; Operating personnel only need simple training to operate.It is highly suitable for that funds are few, small scale but the laboratories of extensively distribution.
3, qualitative and quantitative analysis is accurate, labour intensity is low.Use the film-making of filter membrane concentration method to improve the diatom recovery, after film transparence, use observation by light microscope, diatom texture and details clear, and do not affect that it is quantitative, ensure that the accuracy of qualitative and quantitative analysis.Film-making amount reduces, and without the need to too much training and operation, and can check captured picture in conjunction with computer automation recognition method, classify and statistical treatment, these significantly can reduce the labour intensity of analyst.
4, applicability is extensive.The present invention is not only applicable to diatom examination, more can extend to other inspection fields.As sample during filter membrane enrichment can select plant counteract appearance, soil digested solusion, gas etc.; Suitable coloring agent, fixing agent, mark etc. can be added to observe specific sample during transparence; Test rating choosing multiple algae, small insects, parasite etc.Therefore, applicability of the present invention extensively, can interdisciplinaryly use.
Embodiment
The present invention is further described below in conjunction with specific embodiment.Unless stated otherwise, the present invention adopts reagent, equipment are the art conventional reagent and equipment.
embodiment 1
A kind of diatom inspection method in medical jurisprudence based on filter membrane: be divided into three steps:
S1. filter membrane enrichment:
The sample (the histoorgan digestion solution after dilution) of 50 ~ 100ml is placed in the suction filtration cup interior beginning suction filtration of simply connected or multi-joint Vacuum filtration device; Vacuum tightness 100 ~ 800mmHg, polyethersulfone (PES) filter membrane diameter 10 ~ 50mm, aperture is 0.1 ~ 1.0 μm.Sample suction filtration adds 50 ~ 200ml ultrapure water when completing continues suction filtration, makes filter membrane surface close to neutral.
S2. film transparence:
On the electric furnace that filter membrane after suction filtration completes is placed in 50 ~ 80 DEG C or baking oven heating 1 ~ 10min make its bone dry; Get non-microslide, center drips 1 ~ 5 acetic acid-eugenol reagent; Acetic acid-eugenol reagent mixes according to the ratio uniform of 1:9 the mixed solution obtained for the eugenol solution of concentration 80 ~ 100% acetic acid solution and concentration 60 ~ 100%.Dried filter membrane is placed in slide center, filter membrane drips again 1 ~ 3 acetic acid-eugenol reagent, add a cover cover glass.
S3. qualitative and quantitative analysis:
Under slide after transparent is placed in optical microscope, observe with 20 times or 40 times of object lens; Artificial cognition mode or computer automation recognition method is adopted to check the diatom on filter membrane or captured picture, classify and statistical treatment.Under artificial cognition mode, as the Mini IMS in some visual field, because enlargement factor limit, the requirement of qualification can not be met, then use 60 times of object lens even 100 times of oily mirrors observe its fine structure.
embodiment 2
A kind of diatom inspection method in medical jurisprudence based on filter membrane: be divided into three steps:
S1. filter membrane enrichment:
The sample (the histoorgan digestion solution after dilution) of 50 ~ 100ml is placed in the suction filtration cup interior beginning suction filtration of simply connected or multi-joint Vacuum filtration device; Vacuum tightness 100 ~ 800mmHg, polyethersulfone (PES) filter membrane diameter 10 ~ 50mm, aperture is 0.1 ~ 1.0 μm.Sample suction filtration adds 50 ~ 200ml ultrapure water when completing continues suction filtration, makes filter membrane surface close to neutral.
S2. film transparence:
On the electric furnace that filter membrane after suction filtration completes is placed in 50 ~ 80 DEG C or baking oven heating 1 ~ 10min make its bone dry; Get non-microslide, center drips 1 ~ 5 acetic acid-eugenol reagent; Acetic acid-eugenol reagent mixes according to the ratio uniform of 1:1 the mixed solution obtained for the eugenol solution of concentration 80 ~ 100% acetic acid solution and concentration 60 ~ 100%.Dried filter membrane is placed in slide center, filter membrane drips again 1 ~ 3 acetic acid-eugenol reagent, add a cover cover glass.
S3. qualitative and quantitative analysis:
Under slide after transparent is placed in optical microscope, observe with 20 times or 40 times of object lens; Artificial cognition mode or computer automation recognition method is adopted to check the diatom on filter membrane or captured picture, classify and statistical treatment.Under artificial cognition mode, as the Mini IMS in some visual field, because enlargement factor limit, the requirement of qualification can not be met, then use 60 times of object lens even 100 times of oily mirrors observe its fine structure.
embodiment 3
A kind of diatom inspection method in medical jurisprudence based on filter membrane: be divided into three steps:
S1. filter membrane enrichment:
The sample (the histoorgan digestion solution after dilution) of 50 ~ 100ml is placed in the suction filtration cup interior beginning suction filtration of simply connected or multi-joint Vacuum filtration device; Vacuum tightness 100 ~ 800mmHg, polyethersulfone (PES) filter membrane diameter 10 ~ 50mm, aperture is 0.1 ~ 1.0 μm.Sample suction filtration adds 50 ~ 200ml ultrapure water when completing continues suction filtration, makes filter membrane surface close to neutral.
S2. film transparence:
On the electric furnace that filter membrane after suction filtration completes is placed in 50 ~ 80 DEG C or baking oven heating 1 ~ 10min make its bone dry; Get non-microslide, center drips 1 ~ 5 acetic acid-eugenol reagent; Acetic acid-eugenol reagent mixes according to the ratio uniform of 9:1 the mixed solution obtained for the eugenol solution of concentration 80 ~ 100% acetic acid solution and concentration 60 ~ 100%.Dried filter membrane is placed in slide center, filter membrane drips again 1 ~ 3 acetic acid-eugenol reagent, add a cover cover glass.
S3. qualitative and quantitative analysis:
Under slide after transparent is placed in optical microscope, observe with 20 times or 40 times of object lens; Artificial cognition mode or computer automation recognition method is adopted to check the diatom on filter membrane or captured picture, classify and statistical treatment.Under artificial cognition mode, as the Mini IMS in some visual field, because enlargement factor limit, the requirement of qualification can not be met, then use 60 times of object lens even 100 times of oily mirrors observe its fine structure.
comparative example 1
A kind of diatom inspection method in medical jurisprudence based on filter membrane: be divided into three steps:
S1. filter membrane enrichment:
The sample (the histoorgan digestion solution after dilution) of 50 ~ 100ml is placed in the suction filtration cup interior beginning suction filtration of simply connected or multi-joint Vacuum filtration device; Vacuum tightness 100 ~ 800mmHg, polyethersulfone (PES) filter membrane diameter 10 ~ 50mm, aperture is 0.1 ~ 1.0 μm.Sample suction filtration adds 50 ~ 200ml ultrapure water when completing continues suction filtration, makes filter membrane surface close to neutral.
S2. film transparence:
On the electric furnace that filter membrane after suction filtration completes is placed in 50 ~ 80 DEG C or baking oven heating 1 ~ 10min make its bone dry; Get non-microslide, center drips 1 ~ 5 acetic acid reagent; Acetic acid reagent is concentration 80 ~ 100% acetic acid solution.Dried filter membrane is placed in slide center, filter membrane drips again 1 ~ 3 acetic acid reagent, add a cover cover glass.
S3. qualitative and quantitative analysis:
Under slide after transparent is placed in optical microscope, observe with 20 times or 40 times of object lens; Artificial cognition mode or computer automation recognition method is adopted to check the diatom on filter membrane or captured picture, classify and statistical treatment.Under artificial cognition mode, as the Mini IMS in some visual field, because enlargement factor limit, the requirement of qualification can not be met, then use 60 times of object lens even 100 times of oily mirrors observe its fine structure.
Owing to adopting 80 ~ 100% acetic acid solutions, the poor effect of Vitrification management is carried out to filter membrane, one is carry out the chronic of Vitrification management with 80 ~ 100% acetic acid solutions to filter membrane, easily turn yellow with the filter membrane after 80 ~ 100% acetic acid solution process in addition, be unfavorable for long-term preservation, so, be not suitable for adopting common optical microscope to observe.
comparative example 2
A kind of diatom inspection method in medical jurisprudence based on filter membrane: be divided into three steps:
S1. filter membrane enrichment:
The sample (the histoorgan digestion solution after dilution) of 50 ~ 100ml is placed in the suction filtration cup interior beginning suction filtration of simply connected or multi-joint Vacuum filtration device; Vacuum tightness 100 ~ 800mmHg, polyethersulfone (PES) filter membrane diameter 10 ~ 50mm, aperture is 0.1 ~ 1.0 μm.Sample suction filtration adds 50 ~ 200ml ultrapure water when completing continues suction filtration, makes filter membrane surface close to neutral.
S2. film transparence:
On the electric furnace that filter membrane after suction filtration completes is placed in 50 ~ 80 DEG C or baking oven heating 1 ~ 10min make its bone dry; Get non-microslide, center drips 1 ~ 5 eugenol reagent; Eugenol reagent is the eugenol solution of concentration 60 ~ 100%.Dried filter membrane is placed in slide center, filter membrane drips again 1 ~ 3 eugenol reagent, add a cover cover glass.
S3. qualitative and quantitative analysis:
Under slide after transparent is placed in optical microscope, observe with 20 times or 40 times of object lens; Artificial cognition mode or computer automation recognition method is adopted to check the diatom on filter membrane or captured picture, classify and statistical treatment.Under artificial cognition mode, as the Mini IMS in some visual field, because enlargement factor limit, the requirement of qualification can not be met, then use 60 times of object lens even 100 times of oily mirrors observe its fine structure.
Due to the poor effect adopting the eugenol solution of 60 ~ 100% filter membrane to be carried out to Vitrification management, one be with 60 ~ 100% eugenol solution the chronic of Vitrification management is carried out to filter membrane, easily turn yellow with the filter membrane after the eugenol solution-treated of 60 ~ 100% in addition, be unfavorable for long-term preservation, so, be not suitable for adopting common optical microscope to observe.
application examples
Use method described in embodiment 1 to carry out detection to died to analyze:
Case 1:
On September 21st, 2013, the pond limit other at certain primary school's lateral gate finds a tool female corpse, about 20 years old age, with it without any certificate article.Be difficult to after corpse table look-up determine the cause of the death and case property.
Adopt nitric acid Ethanol Method to clear up during diatom examination, centrifugal enrichment method is in conjunction with observation by light microscope, and result detects the diatom of type identical with pond from lung, and content is 1086/10g lung tissue, does not detect diatom in liver and renal tissue.Then a kind of filter membrane enrichment of rear use, transparence, om observation technical checking diatom will be cleared up with a tissue samples, the diatom with identical type in the water sample of pond is all detected from the dead's lung, liver, kidney, content is respectively 1275/10g lung tissue, 61/10g hepatic tissue, 8/10g nephridial tissue, therefore identifies that this women's cause of the death is for being drowned.In conjunction with other investigation result, finally confirm that the dead is unexpected overboard death.
Case 2:
On March 6th, 2013, the river levee that certain middle school's basketball court is other finds the unknown female's corpse of a tool, corpse height is corrupt, for confirming that the dead is thrown in water be before death drowned or after death entered water by throwing corpse, getting lung, liver and nephridial tissue after autopsy, adopting nitric acid Ethanol Method to clear up, centrifugal enrichment method is in conjunction with observation by light microscope, result detects the diatom of type identical with river from lung, and content is 1762/10g lung tissue, does not detect diatom in liver and renal tissue.Then a kind of filter membrane enrichment of rear use, transparence, om observation technical checking diatom will be cleared up with a tissue samples, the diatom of type identical with river is all detected from the dead's lung, liver, content is respectively 1265/10g lung tissue, 11/10g hepatic tissue, nephridial tissue does not detect diatom, according to this assay, in conjunction with autopsy finding, identify that this women's cause of the death is for being drowned.Confirm after cracking of cases: the dead walks to certain doorway, middle school evening on March 1st, 2013, Lee hidden in the shadow finds one people walking and carries with oneself a knapsack on the arm.Lee is leapt up out suddenly and is robbed Qi Bao, and in the process battled with the dead, goner " chokes " (actual be only of short duration suffocating go into a coma) to death, and then goner pulls neighbouring korneforos into, and the bag with the dead is escaped.
Claims (5)
1. based on a diatom inspection method in medical jurisprudence for filter membrane, it is characterized in that, comprise the steps:
S1. filter membrane enrichment: adopt Vacuum filtration device that check sample is carried out vacuum filtration; Add ultrapure water after sample suction filtration completes and continue suction filtration, make filter membrane surface close to neutral;
S2. film transparence: be placed on microslide by filter membrane drying, drips acetic acid-eugenol reagent on filter membrane, covered;
S3. qualitative and quantitative analysis: the filter membrane after transparent is placed in optical microphotograph Microscopic observation; Artificial cognition mode or computer automation recognition method is adopted to check the diatom on filter membrane or captured picture, classify and statistical treatment.
2. according to claim 1 based on the diatom inspection method in medical jurisprudence of filter membrane, it is characterized in that, the digestion solution that check sample described in S1 is water sample, tracheal strips drowns liquid or histoorgan.
3. according to claim 1 based on the diatom inspection method in medical jurisprudence of filter membrane, it is characterized in that, the method for filter membrane drying described in S2 is: on the electric furnace that the filter membrane after being completed by suction filtration is placed in 50 ~ 80 DEG C or baking oven heating 1 ~ 10min make its bone dry.
4. according to claim 1 based on the diatom inspection method in medical jurisprudence of filter membrane, it is characterized in that, the acetic acid solution that acetic acid described in S2-eugenol reagent is volumetric concentration 80 ~ 100% and the mixed solution that the eugenol solution of volumetric concentration 60 ~ 100% obtains by the volume ratio Homogeneous phase mixing of 1:9 ~ 9:1.
5. according to claim 1 based on the diatom inspection method in medical jurisprudence of filter membrane, it is characterized in that, described in S1, suction filtration vacuum tightness is: 100 ~ 800mmHg, and described filter membrane is the filter membrane of polyethersulfone material, and filter membrane diameter is 10 ~ 50mm, aperture is 0.1 ~ 1.0 μm.
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| CN105784705B (en) * | 2016-02-21 | 2018-05-18 | 山东政法学院 | A kind of diatom inspection method in medical jurisprudence |
| CN110033445A (en) * | 2019-04-10 | 2019-07-19 | 司法鉴定科学研究院 | Medicolegal examination automatic identification system and recognition methods based on deep learning |
| CN113252413B (en) * | 2021-04-16 | 2023-04-11 | 成都新基因格生物科技有限公司 | Diatom inspection method |
| CN116990297B (en) * | 2023-09-27 | 2024-01-02 | 佳木斯大学 | Forensic science diatom check-out set based on filter membrane |
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| JP3806374B2 (en) * | 2002-07-16 | 2006-08-09 | アイシン精機株式会社 | Aldehyde removal member with life indication |
| CN101776623B (en) * | 2009-12-31 | 2012-02-08 | 广州市刑事科学技术研究所 | Diatom inspection method in medical jurisprudence |
| CN102507585B (en) * | 2011-11-11 | 2013-10-30 | 广州市刑事科学技术研究所 | Forensic medical diatom inspection method based on mixed acid digestion |
| US20130078665A1 (en) * | 2012-07-18 | 2013-03-28 | Deepika Bodapati | Test strip, a test kit and a method for detection of endotoxin in food |
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