CN105784644B - A kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine application method - Google Patents
A kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine application method Download PDFInfo
- Publication number
- CN105784644B CN105784644B CN201610296613.3A CN201610296613A CN105784644B CN 105784644 B CN105784644 B CN 105784644B CN 201610296613 A CN201610296613 A CN 201610296613A CN 105784644 B CN105784644 B CN 105784644B
- Authority
- CN
- China
- Prior art keywords
- total internal
- internal reflection
- detection
- light source
- receiver
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N2021/555—Measuring total reflection power, i.e. scattering and specular
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/02—Mechanical
- G01N2201/023—Controlling conditions in casing
- G01N2201/0231—Thermostating
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/02—Mechanical
- G01N2201/023—Controlling conditions in casing
- G01N2201/0233—Gas purge
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/02—Mechanical
- G01N2201/023—Controlling conditions in casing
- G01N2201/0238—Moisture monitoring or controlling
Abstract
The invention discloses a kind of constant temperature total internal reflection micro-fluidic chips to detect all-in-one machine application method, and the application method includes opening host babinet and processing display power supply；Fan, oven heats device, air cleaning unit are opened, increase checking chip adjusts total internal reflection light source and receiver and inverted fluorescence microscope, detection, shutdown.It is an advantage of the present invention to provide can enough total internal reflection detection methods and inverted fluorescence microscope detection method, realize a tractor serves several purposes, meet a machine translocation difference factor requirement.
Description
Technical field
More specifically it is that belong to Medical Devices external the present invention relates to a kind of application method of in-vitro diagnosis all-in-one machine
A kind of constant temperature total internal reflection micro-fluidic chip of diagnostic field detects all-in-one machine application method.
Background technology
In-vitro diagnosis refers to the diagnosis for being detected blood, body fluid, tissue samples after being taken out in human body and carrying out, mesh
It is preceding clinically 80% or more medical diagnosis on disease depend on IVD, be known as " eyes " of doctor.IVD plays more in modern society
Care diagnostic is sent out in the overall process of disease prevention, diagnosis, monitoring and guiding treatment before coming more important role, especially bed
Extremely important effect is waved, is modern diseases and the indispensable tool of health control.Due to micro-fluidic chip obtained it is fast
Speed development, and it is still manufacture processing and the application aspect for limiting its development early stage to hinder the bottleneck of microflow control technique development today
The problem of.Total internal reflection optical detection technique is exactly one and meets the optical detective technology integrated with micro-fluidic chip, still, mesh
It is preceding using both technology detect living cells when, lack meet living cells detection constant temperature all-in-one machine and its application method.
For polyfactorial disposable checkout and diagnosis such as living cells to cancer and cardiovascular and cerebrovascular disease, a kind of constant temperature is needed
Total internal reflection micro-fluidic chip detects the application method of all-in-one machine and it.
Invention content
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of detections of constant temperature total internal reflection micro-fluidic chip
All-in-one machine application method.
The object of the present invention is achieved like this：A kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine user
Method, a kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine, by host babinet and processing display two parts group
At, it is characterised in that：Be processed into closed host babinet, it is vertical it is facing forward have opening on one side, pass through upper nowel inside babinet
Box house is divided into two layers of lower layer's chamber and upper layer chamber by room partition board, and all each comfortable box opening be equipped with it is independent
Lower layer's transparent doors and upper layer transparent doors.Design does not interfere with each other convenient for two operation intervals in this way, avoids pollution effect.Lower layer's case
Room is equipped with inverted fluorescence microscope, the opposite observation window in inverted fluorescence microscope camera lens top.Disposing inverted fluorescence microscope is
Meet a tractor serves several purposes purpose, enough total internal reflections detection and can be detected with inverted fluorescence microscope.Above observation window
The upper surface of the partition board of chamber up and down, i.e., sample stage is installed in upper layer case indoor chamber partition board side up and down；It is solid on sample stage
Surely detection chip is placed；There are total internal reflection light source and receiver right over sample stage, can realize the detection of total internal reflection.
Perpendicular rail is installed on the inboard box body wall of upper layer chamber, traverse rod, traverse rod and perpendicular rail right-angled intersection are also horizontally installed on perpendicular rail
It is mutually perpendicular to, total internal reflection light source and receiver are fixed by perpendicular rail or traverse rod.Design disclosure satisfy that total internal reflection light source in this way
And receiver is vertically moved and is fixed on perpendicular rail, additionally it is possible to meet total internal reflection light source and the receiver left and right on traverse rod
It is mobile and fixed, ensure being smoothed out for total internal reflection detection.The company of being equipped with also is processed on the inboard box body wall of upper layer chamber
The purpose of humidifier and fan of body, the humidifier and fan design of disjunctor, which is fan, to control rotating speed by inductor, to ensure
Living cells detects the constant of required humidity.The inboard box body wall of upper layer chamber has two layers of thermal-insulation baffle plate, is kept the temperature at two layers
It is heating room between partition board, heating indoor location has the electric heater unit that can adjust and control temperature, ensured living cells inspection
Survey required temperature.In host top of the box, processing is equipped with air cleaning unit and is communicated with upper layer chamber, has ensured monitoring
The cleannes requirement in region.Inverted fluorescence microscope and total internal reflection light source in processing display and mainframe box body and receiver
Circuit is connected, and can control two kinds of detection devices up and down.
The application method is as follows.
The first step opens host babinet and processing display power supply；Keep lower layer's transparent doors and upper layer transparent doors all closed
It closes, opens fan, oven heats device, air cleaning unit, operate 15min-30min.
Second step after the temperature and humidity to stability requirement for observing processing display, opens upper layer transparent doors, will detect core
Piece is placed on sample stage, the interface of connecting detection chip and each conduit；According to the detection zone position in detection chip, adjustment
Detection zone position is adjusted to observation window by mobile example platform, fixed.
Third walks, and opens total internal reflection light source and receiver power supply, total internal reflection light source and receiver are raised in perpendicular rail
Whole height, makes it be totally reflected in detection chip, fixed total internal reflection light source and receiver, then by traverse rod be adjusted to
Total internal reflection light source and receiver sustained height are fixed, on traverse rod, then left and right adjustment total internal reflection light source and receiver, make it
It is totally reflected the detection zone position in detection chip, finally fixes total internal reflection light source and receiver, carries out total internal reflection examination
Sample detects, and processing display controls test parameter and gathered data.
4th step when having multiple detection zone positions in detection chip, repeats third step operation.
5th step；Total internal reflection light source and receiver power supply are closed, inverted fluorescence microscope power supply is opened, it is aobvious by handling
Show that device adjusts focal length, shift position makes the detection zone in alignment lens detection chip, carries out conventional fluorescent detection, and processing is aobvious
Show device control fluorescence parameter and gathered data；Conventional fluorescent detection is not needed such as, directly from third step or the 4th step to the 6th step.
6th step；Total internal reflection light source and receiver power supply and inverted fluorescence microscope power supply are first closed, takes detection core away
Piece closes the power supply of fan, oven heats device, air cleaning unit, takes the water in humidifier away successively, finally closes master
Box body and processing display power supply.
Further, the fan is communicated with heating room；The top for heating room is filled by air filtration layer and air purification
Set and communicate, ensured the temperature of upper layer chamber uniformly with it is constant, humidity it is uniform with it is constant.
Further, the host babinet is the closed case processed by thermal insulation material；Lower layer's transparent doors and upper layer
Transparent doors are sealed thermal insulating doors.This ensure that in case the temperature of detection zone upper layer chamber uniformly with it is constant, humidity it is equal
It is even.
Further, the host babinet is cubic shaped or cylindrical shape.It is not limited to conventional chest cuboid
Limitation.
The present invention has the following advantages compared with the prior art.
1. enough total internal reflections detection and can be detected with inverted fluorescence microscope, realizes a tractor serves several purposes, meet
The requirement of the one machine translocation difference factor.
2. combining the integration with all-in-one machine by two kinds of total internal reflection detection technique, microfluidic chip technology technologies, realize
The temperature of detection zone uniformly with it is constant, humidity it is uniform with it is constant.
Description of the drawings
Fig. 1 is the schematic front view of the present invention.
Fig. 2 is the left view schematic diagram of the present invention.
Wherein：1. host babinet；2. lower layer's chamber；3. inverted fluorescence microscope；4. observation window；Chamber partition board about 5.；
6. sample stage；7. humidifier；8. fan；9. traverse rod；10. total internal reflection light source and receiver；11. air cleaning unit；12. perpendicular
Rail；13. upper layer chamber；14. upper layer transparent doors；15. detection chip；16. incident optical alignment scale；17. lower layer's transparent doors；18.
Processing display；19. thermal-insulation baffle plate；20. heating room；21. air filtration layer.
Specific implementation mode
Referring to Fig. 1, Fig. 2, a kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine is aobvious by host babinet 1 and processing
Show that device 18 forms, it is characterised in that：Closed host babinet 1 it is vertical it is facing forward have opening on one side, pass through upper nowel inside babinet
Box house is divided into two layers of lower layer's chamber 2 and upper layer chamber 13 by room partition board 5, and all each comfortable box opening is equipped with solely
Vertical lower layer's transparent doors 17 and upper layer transparent doors 14；Lower layer's chamber 2 is equipped with inverted fluorescence microscope 3, inverted fluorescence microscope 3
Against observation window 4 above camera lens；The upper surface of the partition board of chamber up and down 5 of 4 top of observation window, i.e., the upper nowel in upper layer chamber 13
Partition board 5 side in room is equipped with sample stage 6；Fixed placement detection chip 15 on sample stage 6；Have right over sample stage 6 anti-in complete
Penetrate light source and receiver 10；Perpendicular rail 12 is installed on the inboard box body wall of upper layer chamber 13, is also horizontally mounted on perpendicular rail 12
There are traverse rod 9, traverse rod 9 to be mutually perpendicular to 12 right-angled intersection of perpendicular rail；Total internal reflection light source and receiver 10 pass through perpendicular rail 12 or traverse rod 9
It is fixed, the fixed total internal reflection light source of mobile adjustment and reception on perpendicular rail 12 or traverse rod 9 according to the needs of detection zone when use
Device 10 realizes that the best transmitting of total internal reflection receives position.On the inboard box body wall of upper layer chamber 13 also processing be equipped with it is wet
Spend the disjunctor fan 8 and humidifier 7 of inductor control.The inboard box body wall of upper layer chamber 13 has two layers of thermal-insulation baffle plate 19, two
It is heating room 20 between floor thermal-insulation baffle plate 19, heats the electric heater unit for being equipped with control temperature in room 20, living cells institute is provided
The temperature needed.Air cleaning unit 11 is installed in the 1 top processing of host babinet to communicate with upper layer chamber 13；Processing display
18 in host babinet 1 inverted fluorescence microscope 3 and total internal reflection light source and 10 circuit of receiver be connected.
The application method is as follows.
The first step opens host babinet 1 and 18 power supply of processing display；Keep lower layer's transparent doors 17 and upper layer transparent doors 14
All closed closing opens fan 8, heating 20 heating device of room, air cleaning unit 11, operates 15min-30min.
Second step, observe processing display 18 temperature and humidity to stability requirement after, open upper layer transparent doors 14, will examine
It surveys chip 15 to be placed on sample stage 6, the interface of connecting detection chip 15 and each conduit；According to the detection zone in detection chip 15
Domain position, detection zone position is adjusted to observation window 4 by adjustment mobile example platform 6, fixed.
Third walks, and total internal reflection light source and 10 power supply of receiver is opened, by total internal reflection light source and receiver 10 in perpendicular rail
Height is adjusted on 12, it is made to be totally reflected in detection chip 15, and fixed total internal reflection light source and receiver 10 then will be horizontal
Rail 9 is adjusted to fix with total internal reflection light source and 10 sustained height of receiver, on traverse rod 9, then left and right adjustment total internal reflection light
Source and receiver 10 make it be totally reflected the detection zone position in detection chip 15, finally fix total internal reflection light source and connect
Device 10 is received, total internal reflection sample detection is carried out, processing display 18 controls test parameter and gathered data.
4th step when having multiple detection zone positions in detection chip 15, repeats third step operation.
5th step；Total internal reflection light source and 10 power supply of receiver are closed, 3 power supply of inverted fluorescence microscope is opened, passes through place
It manages display 18 and adjusts focal length, shift position makes the detection zone in alignment lens detection chip 15, carries out conventional fluorescent inspection
It surveys, processing display 18 controls fluorescence parameter and gathered data；Conventional fluorescent detection is not needed such as, directly from third step or the 4th
It walks to the 6th step.
6th step；Total internal reflection light source and 3 power supply of 10 power supply of receiver and inverted fluorescence microscope are first closed, takes detection away
Chip 15 closes the power supply of fan 8, heating 20 heating device of room, air cleaning unit 11, takes the water in humidifier 7 away successively,
Finally close host babinet 1 and 18 power supply of processing display.
The fan 8 of above-mentioned application method, optimization is communicated with heating room 20；The top of heating room 20 passes through air filtration
Layer 21 is communicated with air cleaning unit 11.Make the air cleaner of upper layer chamber 13 and keep certain wind pressure, prevents pollution detection area
Domain.
The host babinet 1 of above-mentioned application method, optimization is the closed case processed by thermal insulation material；The lower layer is saturating
Bright door 17 and upper layer transparent doors 14 are sealed thermal insulating doors.
The host babinet 1 of above-mentioned application method, optimization is cubic shaped or cylindrical shape.
Claims (4)
1. a kind of constant temperature total internal reflection micro-fluidic chip detects all-in-one machine application method, a kind of constant temperature total internal reflection is micro-fluidic
Chip detects all-in-one machine, by host babinet（1）And processing display（18）Composition, host babinet（1）It is facing forward vertically to have on one side
Opening, babinet the inside pass through upper and lower chamber partition board（5）Box house is divided into lower layer's chamber（2）With upper layer chamber（13）Two layers,
And all each comfortable box opening is equipped with independent lower layer's transparent doors（17）With upper layer transparent doors（14）；Lower layer's chamber（2）
Inverted fluorescence microscope is installed（3）, inverted fluorescence microscope（3）Against observation window above camera lens（4）；Observation window（4）Top
Chamber partition board up and down（5）The upper surface of, i.e., in upper layer chamber（13）Interior chamber partition board up and down（5）Side is equipped with sample stage
（6）；Sample stage（6）Upper fixed placement detection chip（15）；In sample stage（6）There are total internal reflection light source and receiver in surface
（10）；In upper layer chamber（13）Inboard box body wall on perpendicular rail is installed（12）, in perpendicular rail（12）On be also horizontally installed with traverse rod
（9）, traverse rod（9）With perpendicular rail（12）Right-angled intersection is mutually perpendicular to；Total internal reflection light source and receiver（10）By erecting rail（12）Or
Traverse rod（9）It is fixed；Processing display（18）With host babinet（1）Interior inverted fluorescence microscope（3）With total internal reflection light source and
Receiver（10）Circuit is connected；It is characterized in that：In upper layer chamber（13）Inboard box body wall on also processing disjunctor is installed
Humidifier（7）And fan（8）；Upper layer chamber（13）Inboard box body wall have two layers of thermal-insulation baffle plate（19）, in two layers of thermal-insulation baffle plate
（19）Between be heating room（20）, heat room（20）The electric heater unit of control temperature is inside installed；In host babinet（1）Top
Processing is equipped with air cleaning unit（11）With upper layer chamber（13）It communicates；
The application method is as follows：
The first step opens host babinet（1）And processing display（18）Power supply；Keep lower layer's transparent doors（17）With upper layer transparent doors
（14）Fan is opened in all closed closing（8）, heating room（20）Heating device, air cleaning unit（11）, operate 15min-
30min；
Second step observes processing display（18）Temperature and humidity to stability requirement after, open upper layer transparent doors（14）, will examine
Survey chip（15）It is placed on sample stage（6）On, connecting detection chip（15）With the interface of each conduit；According to detection chip（15）On
Detection zone position, adjust mobile example platform（6）Detection zone position is adjusted to observation window（4）, fixed；
Third walks, and opens total internal reflection light source and receiver（10）Power supply, by total internal reflection light source and receiver（10）In perpendicular rail
（12）Upper adjustment height, makes it in detection chip（15）It is upper to be totally reflected, fixed total internal reflection light source and receiver（10）, so
Afterwards by traverse rod（9）It is adjusted to and total internal reflection light source and receiver（10）Sustained height is fixed, in traverse rod（9）On, then left and right tune
Whole total internal reflection light source and receiver（10）, make its total reflection in detection chip（15）On detection zone position, finally fix
Total internal reflection light source and receiver（10）, carry out total internal reflection sample detection, processing display（18）It controls test parameter and adopts
Collect data；
4th step, detection chip（15）On when having multiple detection zone positions, repeat third step operation；
5th step；Close total internal reflection light source and receiver（10）Power supply opens inverted fluorescence microscope（3）Power supply passes through place
Manage display（18）Focal length is adjusted, shift position makes alignment lens detection chip（15）On detection zone, carry out conventional fluorescent
Detection, processing display（18）Control fluorescence parameter and gathered data；Do not need such as conventional fluorescent detection, directly from third step or
4th step to the 6th step；
6th step；First close total internal reflection light source and receiver（10）Power supply and inverted fluorescence microscope（3）Power supply takes detection away
Chip（15）, fan is closed successively（8）, heating room（20）Heating device, air cleaning unit（11）Power supply, take humidifier away
Water in 7 finally closes host babinet（1）And processing display（18）Power supply.
2. a kind of constant temperature total internal reflection micro-fluidic chip according to claim 1 detects all-in-one machine application method, feature
It is：The fan（8）With heating room（20）It communicates；Heat room（20）Top pass through air filtration layer（21）With air purification
Device（11）It communicates.
3. a kind of constant temperature total internal reflection micro-fluidic chip according to claim 1 detects all-in-one machine application method, feature
It is：The host babinet（1）It is the closed case processed by thermal insulation material；Lower layer's transparent doors（17）With upper layer transparent doors
（14）It is sealed thermal insulating door.
4. a kind of constant temperature total internal reflection micro-fluidic chip according to claim 1 detects all-in-one machine application method, feature
It is：The host babinet（1）It is cubic shaped or cylindrical shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610296613.3A CN105784644B (en) | 2016-05-08 | 2016-05-08 | A kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610296613.3A CN105784644B (en) | 2016-05-08 | 2016-05-08 | A kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine application method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105784644A CN105784644A (en) | 2016-07-20 |
| CN105784644B true CN105784644B (en) | 2018-07-31 |
Family
ID=56400846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610296613.3A Active CN105784644B (en) | 2016-05-08 | 2016-05-08 | A kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine application method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105784644B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1629619A (en) * | 2003-12-18 | 2005-06-22 | 中国科学院大连化学物理研究所 | Measuring method for migration rate in unimolecular level |
| WO2009013220A1 (en) * | 2007-07-20 | 2009-01-29 | Eppendorf Ag | Detection and/or quantification of target molecules on a solid support |
| CN102954938A (en) * | 2011-08-29 | 2013-03-06 | 中国科学院电子学研究所 | Absorption luminosity detecting sensor based on micro-fluid control channel full-reflection integration light waveguide |
| CN103267543A (en) * | 2013-04-12 | 2013-08-28 | 中国计量学院 | Microscopic particle image micro-flow measuring instrument and method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8329117B2 (en) * | 2009-05-14 | 2012-12-11 | Canon U.S. Life Sciences, Inc. | Microfluidic chip features for optical and thermal isolation |
-
2016
- 2016-05-08 CN CN201610296613.3A patent/CN105784644B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1629619A (en) * | 2003-12-18 | 2005-06-22 | 中国科学院大连化学物理研究所 | Measuring method for migration rate in unimolecular level |
| WO2009013220A1 (en) * | 2007-07-20 | 2009-01-29 | Eppendorf Ag | Detection and/or quantification of target molecules on a solid support |
| CN102954938A (en) * | 2011-08-29 | 2013-03-06 | 中国科学院电子学研究所 | Absorption luminosity detecting sensor based on micro-fluid control channel full-reflection integration light waveguide |
| CN103267543A (en) * | 2013-04-12 | 2013-08-28 | 中国计量学院 | Microscopic particle image micro-flow measuring instrument and method |
Non-Patent Citations (2)
| Title |
|---|
| Experimental and numerical investigation into the joule heating effect for electrokinetically driven microfluidic chips utilizing total internal reflection fluorescence microscopy;Lung-Ming Fu等;《Microfluid Nanofluid》;20080722;第6卷;第499-507页 * |
| Versatile microfluidic total internal reflection (TIR)-based devices: Application to microbeads velocity measurement and single molecule detection with upright and inverted microscope;Nam Cao Hoai Le等;《Lab on a Chip》;20081023;第9卷;第244-250页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105784644A (en) | 2016-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6619886B2 (en) | Smart toilet that can monitor stool | |
| CN105717090B (en) | A kind of integrated total internal reflection micro-fluidic chip detection all-in-one machine | |
| CN206641842U (en) | A kind of toy fluoroscopic imaging device applied to upper conversion | |
| CN108814555B (en) | Finite element rapid image reconstruction system and method for photoacoustic breast imager | |
| CN108392656A (en) | A kind of disinfecting box of medical appliance | |
| CN105784644B (en) | A kind of constant temperature total internal reflection micro-fluidic chip detection all-in-one machine application method | |
| CN104873994A (en) | Disinfecting and heating device for low-to-moderate-dangerousness medical equipment and using method of disinfecting and heating device | |
| CN108523847A (en) | Optoacoustic breast imaging system and its imaging method | |
| CN105784663B (en) | A kind of constant temperature total internal reflection micro-fluidic chip detects all-in-one machine | |
| CN108359639B (en) | Circulating tumor cell capturing system | |
| CN205593923U (en) | Constant temperature total internal reflection micro -fluidic chip detects all -in -one | |
| KR20170069040A (en) | Cell incubator having plate for cell incubation | |
| CN105891178B (en) | A kind of integrated total internal reflection micro-fluidic chip detection all-in-one machine application method | |
| CN108562469A (en) | A kind of clinical medical inspection smear device | |
| CN207618305U (en) | A kind of medical test sample panel storage box | |
| CN109187163A (en) | A kind of endoscope center fixes device with Pathologic specimen steam | |
| CN209559896U (en) | A kind of Enzyme-linked washing-board sample-adding all-in-one machine | |
| CN205593924U (en) | It detects all -in -one to integrate total internal reflection micro -fluidic chip | |
| CN107345899A (en) | A kind of total internal reflection micro-fluidic chip detects all-in-one | |
| CN204745049U (en) | Disinfection heating apparatus of dangerous medical equipment of well minuent | |
| CN208876495U (en) | Optoacoustic breast imaging system | |
| CN208526648U (en) | A kind of automatic viewing device of thermostatic constant wet chamber | |
| CN108344615B (en) | Circulating tumor cell capturing system and control method | |
| CN207085971U (en) | A kind of micro-biological samples handle check-out console | |
| CN101995475A (en) | Method for diagnosing breast cancer by expression of SOX2 protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |